A kind of beef gelatin food promoting pet joint health and preparation method thereof

Abstract

The present application relates to the field of food processing technology, and specifically discloses a kind of bovine gelatin food that promotes the joint health of pets and its preparation method. A kind of bovine gelatin food that promotes the joint health of pets, according to weight parts, raw materials include 70-90 parts of bovine active extract, 15-25 parts of bovine collagen, 3-9 parts of starch, 2-6 parts of pea protein, 0.5-1.5 parts of glycerol, 0.05-0.2 parts of prebiotics and 0.05-0.5 parts of sweetener, and the bovine active extract is derived from bovine bones and cowhide. The bovine gelatin food that promotes the joint health of pets of this application can be used to prepare pet food, can be better digested and absorbed by pets, helps to promote the joint health of pets, and meets the specific nutritional and health needs of pets.

Description

Technical Field

[0001] The present application relates to the technical field of food processing, and more specifically, to a bovine gelatin food for promoting pet joint health and a preparation method thereof. Background Art

[0002] With the continuous development of the pet market and the increasing attention of pet owners to the health of their pets, the demand for functional pet food is growing. As a kind of animal glue, bovine glue has rich nutritional value, including protein, fat, vitamins, calcium, phosphorus, iron and other nutrients. In particular, it contains a large amount of collagen, which plays an important role in enhancing skin elasticity and improving the health of joint cartilage.

[0003] Pet joint health is an important part of pet health maintenance, especially for medium and large dog breeds and elderly pets. As they age, joint wear and inflammation are particularly prominent. With the increasing awareness of pet owners about pet health and the increasing trend of pet aging, the development of bovine gelatin series pet food has broad market prospects and practical significance for effectively promoting pet joint health and meeting pets' specific nutritional and health needs.

[0004] With respect to the above-mentioned related technologies, the inventors found that traditional beef gelatin often adds more auxiliary materials and additives during the preparation process to improve its taste and preservation. However, for some animals, this may cause problems such as indigestion and gastrointestinal discomfort in pets. Especially for pets with fragile digestive systems, such as small cats and dogs, excessive intake of beef gelatin food is difficult to digest and may have adverse effects on health. Summary of the invention

[0005] In order to retain more nutrients in beef gelatin, promote pets' digestion and absorption of beef gelatin food, and enhance the role of beef gelatin food in promoting pets' joint health, the present application provides a beef gelatin food that promotes pets' joint health and a preparation method thereof.

[0006] In the first aspect, the present application provides a bovine gelatin food that promotes pet joint health, using the following technical solution: A bovine gelatin food for promoting joint health of pets. The raw materials include 70-90 parts of bovine active extract, 15-25 parts of bovine collagen, 3-9 parts of starch, 2-6 parts of pea protein, 0.5-1.5 parts of glycerol, 0.05-0.2 parts of prebiotics and 0.05-0.5 parts of sweetener, based on weight. The bovine active extract is derived from bovine bones and cowhide.

[0007] By adopting the above technical scheme, cowhide and cow bones are rich in collagen, chondroitin, glucosamine and other ingredients that are beneficial to joint health. There are no excessive auxiliary materials in the formula of this application. The main ingredients are bovine active extract and bovine collagen in appropriate proportions, starch, pea protein and glycerin are auxiliary materials, and sweeteners and prebiotics are additives. This helps to shape the food, give the food a taste that is favored by pets, and enhance the palatability and digestibility of the food.

[0008] Optionally, the sweetener comprises chitosan and oligofructose in a mass ratio of 1:(0.5-1).

[0009] By adopting the above technical solution, chitosan and oligofructose as dietary fiber sweeteners can not only give food a palatable sweetness and improve the taste and flavor of food, but also contribute to intestinal health and promote digestion and absorption of pets. Chitosan and oligofructose are both high-quality dietary fiber sources. Chitosan has the function of regulating the balance of intestinal microecology, can enhance the immunity of pets and promote digestion and absorption, while oligofructose is also a high-quality prebiotic that can promote the growth of beneficial bacteria in the intestines of pets, maintain the balance of intestinal microecology, further enhance the digestive function of pets, and improve immunity.

[0010] Optionally, the starch is selected from any one of potato starch or corn starch.

[0011] By adopting the above technical scheme, both starches are easy to digest and absorb, which helps pets to better utilize the nutrients in the cow gelatin food. At the same time, they also serve as a molding agent for food, helping to maintain the shape and taste of the food.

[0012] Optionally, the method for preparing the bovine active extract comprises the following steps: S1: Wash and crush cowhide and cow bones, add water, edible alkali and salt, stir and mix, soak and defatted, remove impurities, wash 2-5 times, drain, and obtain defatted cow by-products; S2: mixing the defatted beef by-product with water at a solid-liquid ratio of 1:(1.5-3), boiling at 80-90° C. for 2-3 h, and then naturally cooling to 45-50° C. to obtain a blend; S3: adjusting the pH of the blend to 8-9, then adding complex enzyme A at 38-42°C, keeping the temperature for 5-7h, inactivating the enzyme at high temperature, and cooling to room temperature to obtain enzymolysis solution A; S4: adjusting the pH of the enzymatic solution A to 6.5-7, then adding the complex enzyme B at 48-55°C, keeping the temperature for 2-3h, inactivating the enzyme at high temperature, and cooling to room temperature to obtain the enzymatic solution B; S5: centrifuging and freeze-drying the enzymatic hydrolyzate B to obtain the prefabricated bovine active substance; S6: Dissolve the prefabricated bovine active substance in water, separate and purify it using a 5 kDa ultrafiltration membrane, and freeze-dry the filtrate below 5 kDa obtained by ultrafiltration to obtain.

[0013] Optionally, the complex enzyme A comprises alkaline protease and trypsin in a mass ratio of 1:(0.5-1.5).

[0014] Optionally, the complex enzyme B comprises papain and serine protease in a mass ratio of 1:(0.8-1.2).

[0015] By adopting the above technical solution, different complex enzymes are used in steps to enzymatically hydrolyze the cowhide and cow bone raw materials, which helps to more gently and effectively decompose different types of proteins during the enzymatic hydrolysis process, releasing more nutrients, such as collagen and chondroitin, while avoiding the destruction of nutrients by severe conditions such as high temperature and high pressure, thereby improving the retention rate of nutrients.

[0016] In a second aspect, the present application provides a method for preparing a bovine gelatin food for promoting pet joint health, using the following technical solution: A method for preparing a beef gelatin food for promoting pet joint health comprises the following steps: The bovine active extract, bovine collagen, starch, pea protein and glycerin are mixed, and then prebiotics and sweeteners are added, and the mixture is continued to be evenly mixed to obtain a solid mixture; The solid mixture is mixed with water at a solid-liquid ratio of 1:(2.5-4), stirred evenly to obtain a prefabricated material, and the prefabricated material is shaped to obtain a beef gelatin food that promotes pet joint health.

[0017] Optionally, the gelatin food for promoting pet joint health may be in the form of any one of a gelatin stick, a gelatin braid or a gelatin ring.

[0018] By adopting the above technical scheme, the preparation process of the present application is simple, and cow glue food in different forms such as cow glue sticks, cow glue braids or cow glue rings is prepared, which not only increases the eating pleasure of pets, but also helps pets chew and digest more naturally, thereby better absorbing the nutrients in the cow glue.

[0019] In summary, this application has the following beneficial effects: 1. The bovine gelatin food of the present application is rich in bovine active extracts, which gives the food a high content of collagen, chondroitin, glucosamine and other ingredients that are beneficial to joint health. It can be used as a pet snack or supplement to promote the joint health of pets. In addition, the present application adds ingredients such as prebiotics to help pets digest and absorb nutrients.

[0020] 2. In this application, oligofructose and chitosan are preferably used as sweeteners. Both are high-quality dietary fibers that can regulate the microecological balance of the intestine, enhance the immunity of pets, and promote digestion and absorption.

[0021] 3. This application adopts a step-by-step enzymatic hydrolysis method and uses different complex enzymes to more gently and effectively decompose different types of proteins, release more nutrients, avoid the long-term impact of severe conditions such as high temperature on the destruction of nutrients, and significantly improve the retention rate of nutrients. DETAILED DESCRIPTION

[0022] The following examples further illustrate the present application in detail. raw material

[0023] Unless otherwise specified, the raw materials used in the examples and comparative examples of this application are all commercially available products, specifically: Bovine collagen, selected from Weiyi peptide, bovine cardiac elastin peptide, T5286; Pea protein, selected from Zize Biotechnology, HPLC ≥ 90%; Prebiotics, inulin, selected from Anhui Chenshan Food Ingredients Co., Ltd., content ≥ 98%; Chitosan, selected from Qiangnuo Food Ingredients Co., Ltd., 032; Fructooligosaccharide, selected from Bailong Chuangyuan, 24020. Preparation Example of Bovine Active Extract

[0024] Preparation Example 1 The bovine active extract and its preparation method comprise the following steps: S1: Wash and crush cowhide and cow bones, add water, sodium cocoyl amino acid and sodium hydroxide in a mass ratio of 15:3.5:4, stir and mix, soak and defatted, remove impurities, wash with water twice, drain and obtain defatted cow by-products; S2: mixing the defatted beef by-product with water at a solid-liquid ratio of 1:1.5, boiling at 80° C. for 2 h, and then naturally cooling to 45° C. to obtain a blend; S3: adjusting the pH of the blend to 8, then adding compound enzyme A at 38°C, keeping the temperature for 5 hours, inactivating the enzyme at high temperature, and cooling to room temperature to obtain enzymatic solution A; compound enzyme A includes alkaline protease and trypsin in a mass ratio of 1:0.5; S4: adjusting the pH of the enzymatic solution A to 6.5, then adding compound enzyme B at 48° C., keeping the temperature for 2 hours, inactivating the enzyme at high temperature, and cooling to room temperature to obtain enzymatic solution B; the compound enzyme B comprises papain and serine protease in a mass ratio of 1:0.8; S5: centrifuging the enzymatic hydrolysate B at 10,000 rpm for 25 min, collecting the supernatant and freeze-drying it to obtain the preformed bovine active substance; S6: Dissolve the prefabricated bovine active substance in pure water, separate and purify it using a 5 kDa ultrafiltration membrane, and freeze-dry the filtrate below 5 kDa obtained by ultrafiltration to obtain the product.

[0025] Preparation Example 2 The bovine active extract and its preparation method comprise the following steps: S1: Wash and crush cowhide and cow bones, add water, sodium cocoyl amino acid and sodium hydroxide in a mass ratio of 15:3.5:4, stir and mix, soak and defatted, remove impurities, wash with water 3 times, drain, and obtain defatted cow by-products; S2: mixing the defatted beef by-product with water at a material-liquid ratio of 1:2, boiling at 80° C. for 2-3 h, and then naturally cooling to 48° C. to obtain a blend; S3: adjusting the pH of the blend to 8.5, then adding compound enzyme A at 40°C, keeping the temperature for 6 hours, inactivating the enzyme at high temperature, and cooling to room temperature to obtain enzymatic solution A; the compound enzyme A includes alkaline protease and trypsin in a mass ratio of 1:1; S4: adjusting the pH of the enzymatic solution A to 7, then adding compound enzyme B at 50° C., keeping the temperature for 2-3 hours, inactivating the enzyme at high temperature, and cooling to room temperature to obtain enzymatic solution B; the compound enzyme B includes papain and serine protease in a mass ratio of 1:1; S5: centrifuging the enzymatic hydrolysate B at 10,000 rpm for 25 min, collecting the supernatant and freeze-drying it to obtain the preformed bovine active substance; S6: Dissolve the prefabricated bovine active substance in pure water, separate and purify it using a 5 kDa ultrafiltration membrane, and freeze-dry the filtrate below 5 kDa obtained by ultrafiltration to obtain the product.

[0026] Preparation Example 3 The bovine active extract and its preparation method comprise the following steps: S1: Wash and crush cowhide and cow bones, add water, sodium cocoyl amino acid and sodium hydroxide in a mass ratio of 15:3.5:4, stir and mix, soak and defatted, remove impurities, wash with water 5 times, drain, and obtain defatted cow by-products; S2: mixing the defatted beef by-product with water at a material-liquid ratio of 1:3, boiling at 90° C. for 2-3 h, and then naturally cooling to 50° C. to obtain a blend; S3: adjusting the pH of the blend to 9, then adding compound enzyme A at 42°C, keeping the temperature for 7 hours, inactivating the enzyme at high temperature, and cooling to room temperature to obtain enzymatic solution A; compound enzyme A includes alkaline protease and trypsin in a mass ratio of 1:1.5; S4: adjusting the pH of the enzymatic solution A to 7, then adding compound enzyme B at 55° C., keeping the temperature for 3 h, inactivating the enzyme at high temperature, and cooling to room temperature to obtain enzymatic solution B; the compound enzyme B comprises papain and serine protease in a mass ratio of 1:1.2; S5: centrifuging the enzymatic hydrolysate B at 10,000 rpm for 25 min, collecting the supernatant and freeze-drying it to obtain the preformed bovine active substance; S6: Dissolve the prefabricated bovine active substance in pure water, separate and purify it using a 5 kDa ultrafiltration membrane, and freeze-dry the filtrate below 5 kDa obtained by ultrafiltration to obtain the product.

[0027] Preparation Example 4 The bovine active extract and its preparation method comprise the following steps: S1: Wash and crush cowhide and cow bones, add water, sodium cocoyl amino acid and sodium hydroxide in a mass ratio of 15:3.5:4, stir and mix, soak and defatted, remove impurities, wash 4 times, drain, and obtain defatted cow by-products; S2: mixing the defatted beef by-product with water at a solid-liquid ratio of 1:2.5, boiling at 85° C. for 2.5 h, and then naturally cooling to 50° C. to obtain a blend; S3: adjusting the pH of the blend to 8, then adding compound enzyme A at 40°C, keeping the temperature for 5 hours, inactivating the enzyme at high temperature, and cooling to room temperature to obtain enzymatic solution A; compound enzyme A includes alkaline protease and trypsin in a mass ratio of 1:1.2; S4: adjusting the pH of the enzymatic solution A to 7, then adding compound enzyme B at 48° C., keeping the temperature for 3 h, inactivating the enzyme at high temperature, and cooling to room temperature to obtain enzymatic solution B; the compound enzyme B comprises papain and serine protease in a mass ratio of 1:1.1; S5: centrifuging the enzymatic hydrolysate B at 10,000 rpm for 25 min, collecting the supernatant and freeze-drying it to obtain the preformed bovine active substance; S6: Dissolve the prefabricated bovine active substance in pure water, separate and purify it using a 5 kDa ultrafiltration membrane, and freeze-dry the filtrate below 5 kDa obtained by ultrafiltration to obtain the product.

[0028] Preparation Example 5 The bovine active extract is different from Preparation Example 1 in that the enzymatic hydrolysis of the composite enzyme A in step S3 is not performed, and only the blend is enzymatically hydrolyzed by the composite enzyme B to obtain an enzymatic hydrolyzate. The other steps are the same as Preparation Example 1.

[0029] Preparation Example 6 The bovine active extract is different from Preparation Example 1 in that the enzymatic hydrolysis of the composite enzyme B in step S4 is not performed, and only the composite enzyme A is used to hydrolyze the blend to obtain an enzymatic solution. The other steps are the same as Preparation Example 1. Example Example 1

[0030] A bovine gelatin food for promoting joint health of pets, wherein the raw materials and amounts are shown in Table 1, wherein the bovine active extract is obtained from Preparation Example 1; the starch is corn starch; and the sweetener is chitosan and oligofructose in a mass ratio of 1:1.

[0031] Table 1

[0032] The method for preparing the above-mentioned beef gelatin food for promoting pet joint health comprises the following steps: S1: mixing bovine active extract, bovine collagen, starch, pea protein and glycerin, adding prebiotics and sweetener, and continuing to mix evenly to obtain a solid mixture; S2: mixing the solid mixture with water at a solid-liquid ratio of 1:3, stirring evenly to obtain a prefabricated material, pressing and shaping the prefabricated material to obtain a gelatin stick-shaped gelatin food for promoting pet joint health. Example 2

[0033] A bovine gelatin food for promoting pet joint health, which is different from Example 1 in that the raw materials and amounts are as shown in Table 1, wherein the bovine active extract is obtained from Preparation Example 2; the starch is potato starch; and the sweetener is chitosan and oligofructose in a mass ratio of 1:0.5.

[0034] The method for preparing the above-mentioned beef gelatin food for promoting pet joint health comprises the following steps: S1: mixing bovine active extract, bovine collagen, starch, pea protein and glycerin, adding prebiotics and sweetener, and continuing to mix evenly to obtain a solid mixture; S2: The solid mixture is mixed with water at a solid-liquid ratio of 1:4, and stirred evenly to obtain a prefabricated material. The prefabricated material is pressed and shaped to obtain a gelatin stick-shaped gelatin food for promoting pet joint health. Example 3

[0035] A bovine gelatin food for promoting pet joint health, which is different from Example 1 in that the raw materials and amounts are as shown in Table 1, wherein the bovine active extract is obtained from Preparation Example 3; the starch is corn starch; and the sweetener is chitosan and oligofructose in a mass ratio of 1:0.8.

[0036] The method for preparing the above-mentioned beef gelatin food for promoting pet joint health comprises the following steps: S1: mixing bovine active extract, bovine collagen, starch, pea protein and glycerin, adding prebiotics and sweetener, and continuing to mix evenly to obtain a solid mixture; S2: mixing the solid mixture with water at a solid-liquid ratio of 1:2.5, stirring evenly to obtain a prefabricated material, pressing and shaping the prefabricated material to obtain a gelatin stick-shaped gelatin food for promoting pet joint health. Example

[0037] A bovine gelatin food for promoting pet joint health, which is different from Example 1 in that the raw materials and amounts are as shown in Table 1, wherein the bovine active extract is obtained from Preparation Example 4; the starch is potato starch; and the sweetener is chitosan and oligofructose in a mass ratio of 1:1.

[0038] The method for preparing the above-mentioned beef gelatin food for promoting pet joint health comprises the following steps: S1: mixing bovine active extract, bovine collagen, starch, pea protein and glycerin, adding prebiotics and sweetener, and continuing to mix evenly to obtain a solid mixture; S2: mixing the solid mixture with water at a solid-liquid ratio of 1:3, stirring evenly to obtain a prefabricated material, pressing and shaping the prefabricated material to obtain a gelatin stick-shaped gelatin food for promoting pet joint health. Example 4

[0039] A bovine gelatin food for promoting pet joint health is different from Example 1 in that the bovine active extract in the raw material is obtained from Preparation Example 5, and the other steps are the same as those in Preparation Example 1. Example 5

[0040] A bovine gelatin food for promoting pet joint health, which is different from Example 1 in that the bovine active extract in the raw material is obtained from Preparation Example 6, and the other steps are the same as Preparation Example 1. Comparative Example

[0041] Comparative Example 1 A bovine gelatin food for promoting pet joint health is different from Example 1 in that no prebiotics are added to the raw materials, and the other steps are the same as those of Preparation Example 1.

[0042] Comparative Example 2 A bovine gelatin food for promoting pet joint health is different from Example 1 in that no sweetener is added to the raw materials, and the other steps are the same as those of Preparation Example 1. Performance testing

[0043] The following relevant performance test was conducted on the bovine gelatin food for promoting pet joint health obtained in Example 1-6 and Comparative Example 1-2.

[0044] 40 dogs with joint inflammation and similar symptoms were selected, and three professional veterinarians scored the joint mobility. The dogs were randomly divided into 8 groups, with 5 dogs in each group. Each group of dogs was fed with the bovine gelatin food for promoting pet joint health obtained in Examples 1-6 and Comparative Examples 1-2. The daily feeding standard was: 120 g of staple food and 30 g of the bovine gelatin food of the present application, fed twice a day. After eight weeks of feeding, the joint mobility was scored again by three professional veterinarians, and the results were recorded in Table 3.

[0045] Table 2

[0046] Table 3

[0047] According to the performance test results in Table 3, it can be seen that the beef gelatin food prepared by the preparation method of the present application has the effect of promoting the health of pet joints, can significantly improve the joint mobility of pets, and plays a role in promoting the treatment of pet arthritis.

[0048] According to the performance test results of Examples 1-4, Examples 5-6 and Comparative Examples 1-2, it can be seen that the sweetener and prebiotics in the formula of the present application are helpful to promote the digestion and absorption of bovine gelatin food by pets. In addition, the use of oligofructose and chitosan as a sweetener can better regulate the microecological balance of the intestine, enhance the immunity of pets, and promote digestion and absorption.

[0049] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A beef gelatin food for promoting pet joint health, characterized in that: The raw materials include 70-90 parts of bovine active extract, 15-25 parts of bovine collagen, 3-9 parts of starch, 2-6 parts of pea protein, 0.5-1.5 parts of glycerol, 0.05-0.2 parts of prebiotics and 0.05-0.5 parts of sweetener in parts by weight, and the bovine active extract is derived from bovine bones and cowhide.

2. The beef gelatin food for promoting pet joint health according to claim 1, characterized in that: The sweetener comprises chitosan and oligofructose in a mass ratio of 1:(0.5-1).

3. The beef gelatin food for promoting pet joint health according to claim 1, characterized in that: The starch is selected from any one of potato starch or corn starch.

4. The beef gelatin food for promoting pet joint health according to claim 1, characterized in that: The method for preparing the bovine active extract comprises the following steps: S1: Wash and crush cowhide and cow bones, add water, edible alkali and salt, stir and mix, soak and defatted, remove impurities, wash 2-5 times, drain, and obtain defatted cow by-products; S2: mixing the defatted beef by-product with water at a solid-liquid ratio of 1:(1.5-3), boiling at 80-90° C. for 2-3 h, and then naturally cooling to 45-50° C. to obtain a blend; S3: adjusting the pH of the blend to 8-9, then adding complex enzyme A at 38-42°C, keeping the temperature for 5-7h, inactivating the enzyme at high temperature, and cooling to room temperature to obtain enzymolysis solution A; S4: adjusting the pH of the enzymatic solution A to 6.5-7, then adding the complex enzyme B at 48-55°C, keeping the temperature for 2-3h, inactivating the enzyme at high temperature, and cooling to room temperature to obtain the enzymatic solution B; S5: centrifuging and freeze-drying the enzymatic hydrolyzate B to obtain the prefabricated bovine active substance; S6: Dissolve the prefabricated bovine active substance in water, separate and purify it using a 5 kDa ultrafiltration membrane, and freeze-dry the filtrate below 5 kDa obtained by ultrafiltration to obtain.

5. The beef gelatin food for promoting pet joint health according to claim 4, characterized in that: The complex enzyme A comprises alkaline protease and trypsin in a mass ratio of 1:(0.5-1.5).

6. The beef gelatin food for promoting pet joint health according to claim 4, characterized in that: The complex enzyme B comprises papain and serine protease in a mass ratio of 1:(0.8-1.2).

7. A method for preparing a beef gelatin food for promoting pet joint health according to any one of claims 1 to 6, characterized in that: The following steps are involved: The bovine active extract, bovine collagen, starch, pea protein and glycerin are mixed, and then prebiotics and sweeteners are added, and the mixture is continued to be evenly mixed to obtain a solid mixture; The solid mixture is mixed with water at a solid-liquid ratio of 1:(2.5-4), stirred evenly to obtain a prefabricated material, and the prefabricated material is shaped to obtain a beef gelatin food that promotes pet joint health.

8. The method for preparing the beef gelatin food for promoting pet joint health according to claim 7, characterized in that: The cow glue food for promoting pet joint health can be in the form of any one of a cow glue stick, a cow glue braid or a cow glue ring.

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