Feed yeast culture as well as preparation method and application thereof

Abstract

The invention provides a feed yeast culture as well as a preparation method and application thereof, and belongs to the technical field of feed raw materials. The feed yeast culture provided by the invention is prepared from the following components in parts by weight: 30-60 parts of bamboo leaves, 10-30 parts of soybean meal, 20-30 parts of bran and 15-20 parts of amino acid residues. According to the invention, strains, beer yeast, cellulase, glycosidase and the components are subjected to combined fermentation. Cheap bamboo leaves are used as a main culture medium, the fermentation cost is reduced, meanwhile, functional substances in the bamboo leaves are released through glycosidase, and the innovative bamboo leaf yeast culture is developed. When the prepared yeast culture is added into animal feed, the laying rate of poultry and the daily gain of livestock can be increased.

Description

Technical Field

[0001] The invention belongs to the technical field of feed raw materials, and in particular relates to a feed yeast culture and a preparation method and application thereof. Background Art

[0002] my country has nearly 6.4 million hectares of bamboo plantations, but bamboo leaves, a byproduct of the bamboo industry, have not been fully developed. Studies have found that bamboo leaves contain active polysaccharides, dietary fiber, flavonoids and other compounds related to livestock and poultry growth, and have significant antioxidant and antibacterial properties. The development of bamboo leaves is also in line with the proposed strategic goal of reducing corn and soybean meal in feed. The cost of extracting active substances from bamboo leaves is very high and is not suitable for use in feed. Bamboo leaves cannot be digested and absorbed when directly added to feed. At present, there are few studies and products in China that use bamboo leaves to develop yeast cultures. Summary of the invention

[0003] In view of this, the object of the present invention is to provide a feed yeast culture and a preparation method and application thereof. The present invention uses cheap bamboo leaves as the main culture medium to reduce the fermentation cost, and uses glycosidase to release functional substances in bamboo leaves to develop an innovative bamboo leaf yeast culture. The yeast culture prepared by the present invention is added to animal diet feed, which is beneficial to improve the egg production rate of poultry and the daily weight gain of livestock.

[0004] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0005] The invention provides a feed yeast culture, which is prepared from the following components in parts by weight: 30-60 parts of bamboo leaves, 10-30 parts of soybean meal, 20-30 parts of bran and 15-20 parts of amino acid residues.

[0006] The present invention also provides a method for preparing the above-mentioned fodder yeast culture, comprising: fermenting the bacterial species, brewer's yeast, cellulase, glycosidase and the above-mentioned components together to obtain the fodder yeast culture.

[0007] Preferably, the bamboo leaves are bamboo leaf powder, and the particle size of the bamboo leaf powder is 60 to 80 meshes.

[0008] Preferably, the bacterial species include at least one of Aspergillus niger, Aspergillus oryzae, lactic acid bacteria and white rot mold.

[0009] Preferably, the inoculation amount of the strain is 1×10 7 ~1×10 8 cfu / g.

[0010] Preferably, the inoculation amount of the brewer's yeast is 1×10 7 ~1×10 8 cfu / g.

[0011] Preferably, the added amounts of the cellulase and glycosidase are 10-40 U / g respectively.

[0012] Preferably, the fermentation temperature is 25-30° C. and the fermentation time is 4-5 days.

[0013] The present invention also provides the use of the above-mentioned feeding yeast culture in preparing feed for improving the egg production rate of poultry.

[0014] The present invention also provides the use of the above-mentioned fodder yeast culture in preparing feed for improving daily weight gain of livestock.

[0015] Aiming at the problems that flavonoids in bamboo leaves are difficult to digest and absorb in animals, the feeding effect is poor and the flavonoids are difficult to absorb, exogenous glycosidase is supplemented during fermentation to convert inactive glycosides into active aglycones.

[0016] The present invention can significantly improve the degradation of bamboo leaf fibers by adding cellulase exogenously.

[0017] The invention can significantly promote the degradation of fiber and lignin, provide a carbon source for yeast, and simultaneously convert inactive glycoside substances into aglycone substances, thereby developing a functional yeast culture for feeding.

[0018] The method of the present invention can achieve a cellulose degradation rate of more than 38%, a lignin degradation rate of more than 20%, a flavonoid glycoside conversion rate of more than 80%, a mannan and β-glucan content of more than 2%, and a yeast viable cell count of more than 1*10 6 . DETAILED DESCRIPTION

[0019] The invention provides a feed yeast culture, which is prepared from the following components in parts by weight: 30-60 parts of bamboo leaves, 10-30 parts of soybean meal, 20-30 parts of bran and 15-20 parts of amino acid residues.

[0020] The present invention has no special limitation on the sources of bamboo leaves, soybean meal, bran and amino acid residues, and conventional commercial products in the art can be used.

[0021] In the present invention, the bamboo leaves are preferably crushed bamboo leaves, and the particle size of the crushed bamboo leaves is preferably 60 to 80 meshes.

[0022] The present invention also provides a method for preparing the above-mentioned fodder yeast culture, comprising: fermenting the bacterial species, brewer's yeast, cellulase, glycosidase and the above-mentioned components together to obtain the fodder yeast culture.

[0023] In the present invention, the bacterial species is preferably at least one of Aspergillus niger, Aspergillus oryzae, lactic acid bacteria and white rot mold. The present invention has no special limitation on the source of the bacterial species, and conventional commercial bacterial species in the art can be used.

[0024] In the present invention, the inoculation amount of the strain is preferably 1×10 7 ~1×10 8 cfu / g.

[0025] The present invention has no special limitation on the source of the beer yeast, and any commercially available product in the art may be used.

[0026] In the present invention, the inoculation amount of the brewer's yeast is preferably 1×10 7 ~1×10 8 cfu / g.

[0027] The present invention has no particular limitation on the sources of the cellulase and glycosidase, and conventional commercial products in the art are preferred.

[0028] In the present invention, the addition amounts of the cellulase and glycosidase are preferably 10 to 40 U / g, respectively.

[0029] In the present invention, the bamboo leaves, soybean meal, bran and amino acid residue are preferably sterilized before fermentation. The present invention has no special limitation on the sterilization method, and conventional sterilization methods in the art can be used.

[0030] The fermentation method of the present invention is preferably pile fermentation, and the pile height is preferably 20 to 30 cm.

[0031] In the present invention, the fermentation temperature is preferably 25-30° C., and the fermentation time is preferably 4-5 days.

[0032] In the present invention, the feed yeast culture prepared as above is preferably dried at low temperature.

[0033] The present invention also provides the use of the above-mentioned feed yeast culture in preparing feed for improving the egg production rate of poultry.

[0034] The present invention also provides the use of the above-mentioned fodder yeast culture in preparing feed for improving daily weight gain of livestock.

[0035] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0036] Example 1

[0037] 30 kg of bamboo leaves crushed to 60 mesh, 10 kg of soybean meal, 20 kg of bran, and 15 kg of amino acid residue were mixed evenly and sterilized; then 1×10 Aspergillus niger was inoculated. 7 cfu / g, brewer's yeast 1×10 7cfu / g; and add 10U / g of cellulase and 10U / g of glycosidase; after piling the above materials to 20cm, ferment them at 25℃ for 4d, and dry them at low temperature to obtain the feed yeast culture.

[0038] Example 2

[0039] The feed yeast culture prepared in Example 1 of the present invention was added to the daily diet at a ratio of 1%, 2%, and 1% per ton of feed and mixed evenly (and replaced the corresponding corn and soybean meal in proportion). The laying hens were randomly grouped and sampled and tested after feeding for 56 days.

[0040] Feed raw material composition (%): corn 61%, soybean meal 25.5%, soybean oil 1.5%, limestone 8%, premix 4% (NaCl 3.2g; VA 8000IU; VD32200 IU; VE 5IU; VK32.5 mg; VB12.5 mg; VB25.0 mg; VB62.0 mg; VB 12 0.2 mg; niacin 50 mg; pantothenic acid 6 mg; folic acid 1.0 mg; copper 5.0 mg; iron 60 mg; manganese 40 mg; zinc 50 mg)

[0041] 1. Breeding experiment: 1% of the culture replaced 0.5% of corn and 0.5% of soybean meal. The average egg weight of laying hens increased by 3.8%, the egg production rate of laying hens increased by 1.1%, the Haugh unit increased by 21.4%, and the egg breakage rate decreased by 5%.

[0042] 2. Breeding experiment: 2% of the culture replaced 1% of corn and 1% of soybean meal. The average egg weight of laying hens increased by 4.1%, the egg production rate of laying hens increased by 2.4%, the Haugh unit increased by 23%, and the egg breakage rate decreased by 13%.

[0043] Example 3

[0044] The feed yeast culture prepared in Example 1 of the present invention was added to the daily feed at a ratio of 2% per ton of feed and mixed evenly (and replaced the corresponding corn and soybean meal in proportion). The pigs were randomly divided into groups and samples were taken for testing after feeding for 60 days.

[0045] Feed raw material composition (%): corn 62%, soybean meal 23%, second powder 8%, oil 3%, premix 4% (VA 1400IU; VD 160IU; VE 13IU; VK 0.6IU; VB11 mg; VB61mg; VB 12 12μg; biotin 55μg; choline 0.3g; pantothenic acid 8mg; folic acid 0.3mg; riboflavin 2.6mg; copper 6mg; iron 100mg; zinc 110mg; manganese 3mg; selenium 0.3mg; iodine 0.14mg)

[0046] Breeding experiment: 2% of culture replaced 1% of corn and 1% of soybean meal, the average daily weight gain of pigs increased by 6.7% and the feed-to-weight ratio decreased by 2.99%.

[0047] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A feed yeast culture, characterized in that: The invention is prepared from the following components in parts by weight: 30 to 60 parts of bamboo leaves, 10 to 30 parts of soybean meal, 20 to 30 parts of bran and 15 to 20 parts of amino acid residues.

2. A method for preparing a feed yeast culture, characterized in that: include: The bacterial species, brewer's yeast, cellulase, glycosidase and the components described in claim 1 are fermented together to obtain a feed yeast culture.

3. A feed yeast culture according to claim 1, characterized in that: The bamboo leaves are bamboo leaf powder, and the particle size of the bamboo leaf powder is 60 to 80 meshes.

4. The method for preparing a feed yeast culture according to claim 2, characterized in that: The bacterial species include at least one of Aspergillus niger, Aspergillus oryzae, lactic acid bacteria and white rot mold.

5. The method for preparing a feed yeast culture according to claim 5, characterized in that: The inoculation amount of the strain was 1×10 7 ~1×10 8 cfu / g.

6. The method for preparing a feed yeast culture according to claim 2, characterized in that: The inoculation amount of the brewer's yeast was 1×10 7 ~1×10 8 cfu / g.

7. The method for preparing a feed yeast culture according to claim 2, characterized in that: The added amounts of the cellulase and glycosidase are 10-40 U / g respectively.

8. The method for preparing a feed yeast culture according to claim 2, characterized in that: The fermentation temperature is 25-30° C. and the fermentation time is 4-5 days.

9. Use of the feed yeast culture according to claim 1 in preparing feed for improving egg production rate of poultry.

10. Use of the feed yeast culture according to claim 1 in preparing feed for improving daily weight gain of livestock.