Additive for preparing pig feed and pig feed

Abstract

The invention relates to the technical field of animal feed, in particular to an additive for preparing pig feed and the pig feed. The additive for preparing the pig feed is obtained by performing enzymolysis on potato protein powder, hermetia illucens powder and cinnamon essential oil through a compound enzyme, and the preparation method comprises the following steps: S1, firstly, adding the potato protein powder, the hermetia illucens powder and the cinnamon essential oil into deionized water, and uniformly mixing to obtain an enzymolysis preparation solution; s2, adding compound protease into the enzymolysis preparation solution, uniformly stirring and mixing, sealing with a preservative film, performing enzymolysis in a water bath environment, performing enzyme deactivation for 10-20 minutes after the enzymolysis is finished, and terminating the reaction to obtain an enzymolysis product; and S3, cooling the enzymolysis product to room temperature, centrifuging, and taking supernate to obtain the additive for preparing the pig feed. The additive for the pig feed can perfectly replace soybean meal and fish meal in traditional pig feed, and meanwhile, the feed intake of live pigs is further increased.

Description

Technical Field

[0001] The present application relates to the technical field of animal feed, and more specifically, to an additive for preparing pig feed and pig feed. Background Art

[0002] Decreased feed intake is a common problem in pig farming. It not only directly affects the growth rate and health status of pigs, but is also significantly related to the economic benefits of the farm. The feed intake of pigs is affected by a variety of factors, including feed quality, feeding environment, disease status and management measures.

[0003] Amino acids are important nutrients for the growth and development of pigs. They cannot be synthesized by the pig's body or the amount synthesized is insufficient, so they must be ingested through feed. Insufficient or unbalanced amino acids in feed will have a great impact on the pig's feed intake.

[0004] Soybean meal and fish meal are the most commonly used animal and plant protein feeds in the feed industry. Both are rich in essential amino acids such as lysine and methionine. However, the production of fish meal and soybean meal is currently on a downward trend worldwide, resulting in a shortage of supply. Therefore, a new protein source is urgently needed to replace soybean meal and fish meal. Summary of the invention

[0005] In order to cope with the current situation of insufficient production of soybean meal and fish meal, the present application provides an additive for preparing pig feed.

[0006] In the first aspect, the present application provides an additive for preparing pig feed, which adopts the following technical solution:

[0007] An additive for preparing pig feed is obtained by enzymolysis of an additive comprising potato protein powder and black soldier fly powder through a composite enzyme.

[0008] Potato protein powder contains much higher protein content than soybean meal, and is rich in amino acids and has a uniform structure. It is rich in lysine, methionine, threonine, tryptophan, etc. At the same time, the antimicrobial peptides contained in potato protein powder can maintain intestinal homeostasis in pigs and regulate inflammatory responses. Therefore, potato protein powder can be used as a plant-based protein to perfectly replace soybean meal.

[0009] Black soldier fly meal has an extremely high protein content, with a crude protein content of 47%-50%, and is rich in active ingredients such as vitamins, minerals and amino acids. At the same time, black soldier fly meal is also rich in antimicrobial peptides, which can activate the host's defense system and kill pathogens. Black soldier fly meal also contains a variety of biologically active substances, such as chitin, which can effectively increase the abundance of flora in the pig intestine and regulate inflammatory responses. In addition, black soldier flies have the characteristics of large egg-laying volume, short development time, and high larval survival rate. Therefore, black soldier fly meal can be used as an animal source protein to perfectly replace fish meal.

[0010] However, potato protein powder and black soldier fly powder have the disadvantage of poor digestibility and utilization by pigs. The reason for the poor digestibility and utilization of potato protein powder may be that potato protein powder contains anti-nutritional factors, such as phytic acid or trypsin inhibitors, which can combine with amino acids, thereby reducing the digestibility and utilization of pigs. In addition to anti-nutritional factors, black soldier fly powder may also contain allergens that cause poor food allergies in pigs, which in turn leads to adverse reactions such as diarrhea in pigs.

[0011] Therefore, in order to improve the digestion and absorption rate of potato protein powder and black soldier fly powder by pigs, the potato protein powder and black soldier fly powder are subjected to enzymatic hydrolysis in the present application, so that the composite enzyme decomposes the macromolecular substances in the potato protein powder and the black soldier fly powder, thereby releasing more and more easily digestible free amino acids and active small molecule peptides, while reducing the concentration of anti-nutritional factors and allergens.

[0012] Preferably, the additive for preparing pig feed is obtained by enzymolysis of an additive comprising potato protein powder, black soldier fly powder and cinnamon essential oil by complex enzyme.

[0013] Disease is one of the important reasons that affect the appetite of pigs. Therefore, antibiotics play an important role in the pig production process. They can promote animal growth and treat related diseases, thereby promoting pig production and bringing economic value. However, the large-scale use of antibiotics will lead to drug resistance and drug residues in pigs, posing hidden dangers to pig production and human health.

[0014] Cinnamon essential oil contains cinnamaldehyde, a natural antibacterial, anti-inflammatory, antioxidant and immunomodulatory ingredient. Cinnamaldehyde has a broad-spectrum antibacterial activity and can act on multiple targets to exert antibacterial activity, including changing the permeability of bacterial cell walls / cell membranes, inhibiting biofilm formation, inhibiting quorum sensing systems, and inhibiting cell metabolism and cell survival.

[0015] Therefore, when cinnamon essential oil is added to pig feed, the cinnamaldehyde in cinnamon essential oil can inhibit the production of pro-inflammatory cytokines and enhance the expression of anti-inflammatory factors. At the same time, it can regulate gastrointestinal function by activating specific TRP channels, thereby having a positive effect on the immune system and protecting cells from oxidative damage, achieving a perfect alternative to antibiotics.

[0016] Preferably, the complex enzyme is a mixture of alkaline protease, trypsin, lipase, xylanase and β-glucanase.

[0017] In the present application, potato protein powder and black soldier fly powder are enzymatically hydrolyzed by using a dual protease in combination with amylase to obtain more enzymatic hydrolysis sites, thereby promoting more complete enzymatic hydrolysis of potato protein powder and black soldier fly powder and effectively increasing the amino acid content. The presence of lipase can reduce the barrier of oil to enzymatic hydrolysis.

[0018] When xylanase and β-glucanase are used in combination with cinnamon essential oil, the number of lymph follicles and mucosal macrophages in pigs will be significantly reduced. At the same time, the expression level of IL-1α in pigs will decrease, which will improve the ileal histology of pigs and help regulate the expression of inflammatory cytokines in the ileum of pigs.

[0019] Preferably, the mass ratio of the potato protein powder, the black soldier fly powder and the cinnamon essential oil is (8-14):(2-4):1.

[0020] Preferably, the mass ratio of the alkaline protease, trypsin, lipase, xylanase and β-glucanase is (8-12): (8-12): (2-4): 1:1.

[0021] Preferably, the method for preparing the additive for pig feed comprises the following steps:

[0022] S1. First, add 10-20 g of potato protein powder, black soldier fly powder and cinnamon essential oil to 40-60 ml of deionized water and mix well to obtain an enzymatic hydrolysis preparatory solution;

[0023] S2, add 0.4-0.6g of composite protease to the preparatory solution for enzymolysis, stir and mix evenly, seal with plastic wrap, and perform enzymolysis in a water bath at 50-70°C for 3-5h. After the enzymolysis is completed, place the enzymolysis solution at 90-100°C for 10-20min to inactivate the enzyme, terminate the reaction, and obtain an enzymolysis product;

[0024] S3. Cool the enzymatic hydrolysis product to room temperature, then centrifuge it at 8-12°C and 4000-6000 rpm for 8-12 minutes, and take the supernatant to obtain an additive for preparing pig feed.

[0025] In a second aspect, the present application provides a pig feed, which adopts the following technical solution:

[0026] A pig feed comprises the following raw materials in parts by weight: 70-80 parts of a basic feed, 6-14 parts of an additive for preparing a pig feed according to any one of claims 1-6, 20-30 parts of a fermented feed and 0.020-0.025 parts of a vitamin premix, wherein the fermented feed is prepared by a solid-state fermentation method using the basic feed as a substrate and a composite probiotic as a fermentation strain.

[0027] Preferably, the composite probiotic is a mixture of Lactobacillus plantarum, yeast and Bacillus subtilis.

[0028] As a new type of green and environmentally friendly feed, fermented feed can improve feed utilization and nutritional level to a certain extent and reduce breeding costs. At the same time, feed fermented with compound probiotics plays an extremely important role in regulating the intestinal microecological balance of pigs, preventing the occurrence of intestinal diseases, promoting pig health and improving the breeding environment.

[0029] Among them, when the compound probiotics are a mixture of Lactobacillus plantarum, yeast and Bacillus subtilis, the acidic environment created by the lactic acid bacteria can promote the proliferation of yeast. At the same time, the vitamins and soluble nitrogen compounds produced by yeast can promote the growth of lactic acid bacteria and Bacillus subtilis. The three complement each other and effectively increase the feed intake of pigs.

[0030] Preferably, the mass ratio of Lactobacillus plantarum, yeast and Bacillus subtilis is (1-3):1:(0.4-0.8).

[0031] Preferably, in the fermented feed, the bacterial inoculation amount is 0.04%-0.10%, the fermentation water content is 40%-50%, the fermentation temperature is 30-50°C, and the fermentation time is 1-2 days.

[0032] In summary, this application has the following beneficial effects:

[0033] 1. Potato protein powder contains much higher protein content than soybean meal, and its amino acid content is rich and uniform in structure. It is rich in lysine, methionine, threonine, tryptophan, etc. At the same time, the antimicrobial peptides contained in potato protein powder can maintain the intestinal homeostasis of pigs and regulate inflammatory response. Therefore, potato protein powder can be used as a plant-based protein to perfectly replace soybean meal.

[0034] 2. The protein content in black soldier fly meal is extremely high, with a crude protein content of 47%-50%, and it is rich in active ingredients such as vitamins, minerals and amino acids. At the same time, black soldier fly meal is also rich in antimicrobial peptides, which can activate the host's defense system and kill pathogens. Black soldier fly meal also contains a variety of biologically active substances, such as chitin, which can effectively increase the abundance of flora in the intestines of pigs and regulate inflammatory responses. In addition, black soldier flies have the characteristics of large egg-laying volume, short development time, and high larval survival rate. Therefore, black soldier fly meal can be used as an animal source protein to perfectly replace fish meal.

[0035] 3. Potato protein powder and black soldier fly powder have the defect of poor digestion and utilization rate of pigs. In order to improve the digestion and absorption rate of potato protein powder and black soldier fly powder by pigs, the potato protein powder and black soldier fly powder are enzymatically hydrolyzed in the present application, so that the composite enzyme decomposes the macromolecular substances in the potato protein powder and black soldier fly powder, thereby releasing more and more easily digestible free amino acids and active small molecule peptides, while reducing the concentration of anti-nutritional factors and allergens.

[0036] 4. When cinnamon essential oil is added to pig feed, the cinnamaldehyde in cinnamon essential oil can inhibit the production of pro-inflammatory cytokines and enhance the expression of anti-inflammatory factors. At the same time, it regulates gastrointestinal function by activating specific TRP channels, thereby having a positive effect on the immune system and protecting cells from oxidative damage, achieving a perfect alternative to antibiotics. DETAILED DESCRIPTION

[0037] The present application is further described in detail below in combination with Application Example 1 to Application Example 14 and Application Comparative Example 1.

[0038] raw material

[0039] Basic feed: 65kg corn, 10kg soybean meal, 2kg fish meal, 5kg whey powder, 2kg soybean oil, 0.88kg calcium hydrogen phosphate, 0.85kg stone powder, and 0.40kg salt.

[0040] The above raw materials, potato protein powder, black soldier fly powder, and cinnamon essential oil are all commercially available;

[0041] Vitamin premix: Shandong Zhonggu Feed Co., Ltd.;

[0042] Alkaline protease CAS: 9014-01-1; Trypsin CAS: 9002-07-7; Lipase CAS: 9001-62-1; Xylanase CAS: 9025-57-4; β-glucanase CAS: 9025-70-1;

[0043] Lactobacillus plantarum ATCC8014; Saccharomyces ATCC 4021258; Bacillus subtilis ATCC9372.

[0044] Example 1

[0045] An additive for preparing pig feed is obtained by enzymolysis of potato protein powder, black soldier fly powder and cinnamon essential oil through composite enzyme.

[0046] The mass ratio of potato protein powder, black soldier fly powder and cinnamon essential oil is 11:3:1, the complex enzyme is a mixture of alkaline protease, trypsin, lipase, xylanase and β-glucanase, and the mass ratio of alkaline protease, trypsin, lipase, xylanase and β-glucanase is 10:10:3:1:1.

[0047] The method for preparing the additive for pig feed comprises the following steps:

[0048] S1. First, add 15 g of potato protein powder, black soldier fly powder and cinnamon essential oil to 50 ml of deionized water and mix well to obtain an enzymatic hydrolysis preparatory solution;

[0049] S2, add 0.5g of composite protease to the preparatory solution for enzymolysis, stir and mix evenly, seal with plastic wrap, and enzymolyze in a water bath at 60°C for 4h. After the enzymolysis is completed, place the enzymolysis solution at 90°C for 20min to inactivate the enzyme, terminate the reaction, and obtain an enzymolysis product;

[0050] S3. Cool the enzymatic hydrolysis product to room temperature, then centrifuge at 10°C and 45,000 rpm for 10 min, and take the supernatant to obtain an additive for preparing pig feed.

[0051] Example 2

[0052] The difference from Example 1 is that cinnamon essential oil is no longer added to the additive for preparing pig feed.

[0053] Example 3-Example 4

[0054] The difference from Example 1 is that the mass ratios of potato protein powder, black soldier fly powder and cinnamon essential oil are different, as shown in Table 1.

[0055] Table 1 Mass ratio of potato protein powder, black soldier fly powder and cinnamon essential oil in Example 1-Example 3

[0056] Example 1 Example 2 Example 3 Potato Protein Powder 11 8 14 Black soldier fly powder 3 4 2 Cinnamon essential oil 1 1 1

[0057] Example 5

[0058] The difference from Example 1 is that lipase, xylanase and β-glucanase are no longer added to the complex enzyme.

[0059] Example 6

[0060] The difference from Example 1 is that alkaline protease is no longer added to the complex enzyme.

[0061] Example 7

[0062] The difference from Example 1 is that trypsin is no longer added to the complex enzyme.

[0063] Example 8-Example 9

[0064] The difference from Example 1 is that the mass ratios of the components of the complex enzyme are different, as shown in Table 2.

[0065] Table 2 Mass ratio of each component of the complex enzyme of Example 1, Example 8 and Example 9

[0066] Example 1 Example 8 Example 9 Alkaline protease 10 8 12 Trypsin 10 12 8 Lipase 3 4 2 Xylanase 1 1 1 β-Glucanase 1 1 1

[0067] Application Example 1-Application Example 9

[0068] A pig feed, comprising the following raw materials: 75 kg of basic feed, 10 kg of the additive for preparing pig feed of Example 1 to Example 9, 25 kg of fermented feed and 0.025 kg of vitamin premix;

[0069] Among them, the fermented feed is prepared by solid-state fermentation method with basic feed as substrate and compound probiotics as fermentation strains. Specifically, the inoculation amount of compound probiotics is 0.07% of the total mass of the substrate, the fermentation water is 40%, the fermentation temperature is 40°C, and the fermentation time is 2d.

[0070] The composite probiotics are a mixture of Lactobacillus plantarum, yeast and Bacillus subtilis, and the mass ratio of Lactobacillus plantarum, yeast and Bacillus subtilis is 2:1:0.6.

[0071] Application Example 10

[0072] The difference from Application Example 1 is that the composite probiotics are replaced with Lactobacillus plantarum in the same added amount.

[0073] Application Example 11

[0074] The difference from Application Example 1 is that the composite probiotics are replaced with yeast in the same added amount.

[0075] Application Example 12

[0076] The difference from Application Example 1 is that the composite probiotics are replaced with the same added amount of Bacillus subtilis.

[0077] Application Example 13-Application Example 14

[0078] The difference from Application Example 1 is that the mass ratios of the components of the composite probiotics are different, as shown in Table 3.

[0079] Table 3 Mass ratio of each component of composite probiotics in Application Example 1 and Application Examples 13-14

[0080] Application Example 1 Application Example 13 Application Example 14 Lactobacillus plantarum 2 1 3 Yeast 1 1 1 Bacillus subtilis 0.6 0.8 0.4

[0081] Application Comparative Example 1

[0082] The difference from Application Example 1 is that the additive for preparing pig feed in Example 1 is replaced by a mixture of soybean meal and fish meal of the same mass, and the mass ratio of soybean meal to fish meal is 4:1.

[0083] Performance testing

[0084] 38-day-old piglets were selected as test subjects, and 45 relatively healthy weaned nursery pigs with similar body weight and similar serum neutrophil phagocytosis were randomly divided into 15 groups, and then fed with pig feeds of Application Example 1 to Application Example 14 and Application Comparative Example 1, respectively, for a test period of 47 days. During the test, the pigs had free access to food and water, and the feeding management and immune health care procedures were normal.

[0085] 1. Pig digestion capacity test

[0086] The fasting body weight of the experimental pigs on the first day and the forty-seventh day was weighed, and then the average weight gain of each experimental group was calculated; then the feed input and residual feed amount were counted daily, the total feed consumption for forty-seven days was calculated, and the average total feed consumption of each experimental group was calculated; finally, the average feed-to-meat ratio was calculated.

[0087] Average feed-to-meat ratio = average total feed consumption / average total weight gain.

[0088] The test data are shown in Table 4.

[0089] Table 4 Test data table of application examples 1 to 14 and comparative example 1

[0090] Average feed to meat ratio Average feed to meat ratio Application Example 1 1.66 Application Example 9 1.67 Application Example 2 1.76 Application Example 10 1.69 Application Example 3 1.68 Application Example 11 1.75 Application Example 4 1.70 Application Example 12 1.77 Application Example 5 1.72 Application Example 13 1.68 Application Example 6 1.76 Application Example 14 1.70 Application Example 7 1.74 Application Comparative Example 1 1.83 Application Example 8 1.69

[0091] Referring to Application Example 1 and Application Comparative Example 1 and in combination with Table 4, it can be seen that the average feed-to-meat ratio of Application Example 1 is significantly improved compared with Application Comparative Example 1, which shows that the additive for preparing pig feed of the present application can effectively replace soybean meal and fish meal, and at the same time, pigs have a better digestion effect on pig feed added with the additive for preparing pig feed.

[0092] The reason is that potato protein powder contains much higher protein content than soybean meal, and is rich in amino acids and has a uniform structure. It is rich in lysine, methionine, threonine, tryptophan, etc. At the same time, the antimicrobial peptides contained in potato protein powder can maintain the homeostasis of the pig intestine and regulate inflammatory response. Therefore, potato protein powder can be used as a plant-based protein to perfectly replace soybean meal.

[0093] Black soldier fly meal has an extremely high protein content, with a crude protein content of 47%-50%, and is rich in active ingredients such as vitamins, minerals and amino acids. At the same time, black soldier fly meal is also rich in antimicrobial peptides, which can activate the host's defense system and kill pathogens. Black soldier fly meal also contains a variety of biologically active substances, such as chitin, which can effectively increase the abundance of flora in the pig intestine and regulate inflammatory responses. In addition, black soldier flies have the characteristics of large egg-laying volume, short development time, and high larval survival rate. Therefore, black soldier fly meal can be used as an animal source protein to perfectly replace fish meal.

[0094] However, potato protein powder and black soldier fly powder have the disadvantage of poor digestibility and utilization by pigs. The reason for the poor digestibility and utilization of potato protein powder may be that potato protein powder contains anti-nutritional factors, such as phytic acid or trypsin inhibitors, which can combine with amino acids, thereby reducing the digestibility and utilization of pigs. In addition to anti-nutritional factors, black soldier fly powder may also contain allergens that cause poor food allergies in pigs, thereby causing diarrhea in pigs.

[0095] In Example 1, the potato protein powder and the black soldier fly powder are subjected to enzymatic hydrolysis, so that the composite enzyme decomposes the macromolecular substances in the potato protein powder and the black soldier fly powder, thereby releasing more and more easily digestible free amino acids and active small molecule peptides, while reducing the concentration of anti-nutritional factors and allergens.

[0096] Referring to Application Example 1 and Application Example 2 and in combination with Table 4, it can be seen that, compared with Application Example 1, the average feed-to-meat ratio of Application Example 2 is significantly improved, which indicates that the addition of cinnamon essential oil can further improve the digestibility of pigs for pig feed.

[0097] The reason is that disease is one of the important factors that affect the appetite and digestive ability of pigs. Therefore, antibiotics play an important role in the pig production process. They can promote animal growth and treat related diseases, thereby promoting pig production and bringing economic value. However, the large-scale use of antibiotics will lead to drug resistance and drug residues in pigs, posing hidden dangers to pig production and human health.

[0098] Cinnamon essential oil contains cinnamaldehyde, a natural antibacterial, anti-inflammatory, antioxidant and immunomodulatory ingredient. Cinnamaldehyde has a broad-spectrum antibacterial activity and can act on multiple targets to exert antibacterial activity, including changing the permeability of bacterial cell walls / cell membranes, inhibiting biofilm formation, inhibiting quorum sensing systems, and inhibiting cell metabolism and cell survival.

[0099] Therefore, when cinnamon essential oil is added to pig feed, the cinnamaldehyde in cinnamon essential oil can inhibit the production of pro-inflammatory cytokines and enhance the expression of anti-inflammatory factors. At the same time, it can regulate gastrointestinal function by activating specific TRP channels, thereby having a positive effect on the immune system and protecting cells from oxidative damage, achieving a perfect alternative to antibiotics.

[0100] With reference to Application Example 1, Application Example 3 and Application Example 4 and in combination with Table 4, it can be seen that, relative to Application Example 1, the average feed-to-meat ratios of Application Example 3 and Application Example 4 are significantly improved, which indicates that when the mass ratios of potato protein powder, black soldier fly powder and cinnamon essential oil are the same as those in Application Example 1, pigs have a better digestion effect on pig feed.

[0101] With reference to Application Example 1, Application Example 5, Application Example 6 and Application Example 7 and in combination with Table 4, it can be seen that, relative to Application Example 1, the average feed-to-meat ratios of Application Example 5, Application Example 6 and Application Example 7 are significantly improved, which indicates that among the complex enzymes, alkaline protease, trypsin, lipase, xylanase and β-glucanase have a certain synergistic effect, and the lack of any part will lead to a significant decrease in the digestion ability of pigs for pig feed.

[0102] The reason is that, in Example 1, the potato protein powder and the black soldier fly powder are enzymatically hydrolyzed by using a dual protease and an amylase, so that more enzymatic hydrolysis sites are obtained, which makes the enzymatic hydrolysis of the potato protein powder and the black soldier fly powder more complete, and effectively increases the amino acid content, while the presence of lipase can reduce the obstacle of oil to enzymatic hydrolysis.

[0103] When xylanase and β-glucanase are used in combination with cinnamon essential oil, the number of lymph follicles and mucosal macrophages in pigs will be significantly reduced. At the same time, the expression level of IL-1α in pigs will decrease, which will improve the histology of the ileum of pigs and help regulate the expression of inflammatory cytokines in the ileum of pigs.

[0104] With reference to Application Example 1, Application Example 8 and Application Example 9 and in combination with Table 4, it can be seen that, relative to Application Example 1, the average feed-to-meat ratios of Application Example 8 and Application Example 9 are slightly improved, which indicates that when the components of the complex enzyme adopt the mass ratio of Application Example 1, pigs will have a better digestion effect on pig feed.

[0105] With reference to Application Example 1, Application Example 10, Application Example 11 and Application Example 12 and in combination with Table 4, it can be seen that, relative to Application Example 1, the average feed-to-meat ratios of Application Example 10, Application Example 11 and Application Example 12 are significantly improved, which indicates that Lactobacillus plantarum, yeast agent Bacillus subtilis have a certain synergistic effect, and the lack of any part will lead to a significant deterioration in the digestion effect of pigs on pig feed.

[0106] The reason is that fermented feed, as a new type of green and environmentally friendly feed, can improve the utilization rate and nutritional level of feed to a certain extent and reduce breeding costs. At the same time, feed fermented with compound probiotics plays an extremely important role in regulating the intestinal microecological balance of pigs, preventing the occurrence of intestinal diseases, promoting pig health and improving the breeding environment.

[0107] Among them, when the compound probiotics are a mixture of Lactobacillus plantarum, yeast and Bacillus subtilis, the acidic environment created by the lactic acid bacteria can promote the proliferation of yeast. At the same time, the vitamins and soluble nitrogen compounds produced by yeast can promote the growth of lactic acid bacteria and Bacillus subtilis. The three complement each other and effectively increase the feed intake of pigs.

[0108] With reference to Application Example 1, Application Example 13 and Application Example 14 and in combination with Table 4, it can be seen that, relative to Application Example 1, the average feed-to-meat ratios of Application Example 13 and Application Example 14 are slightly improved, which indicates that when the components of the composite probiotics adopt the mass ratio of Application Example 1, the digestion effect of pigs on pig feed is better.

[0109] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. An additive for preparing pig feed, characterized in that: The invention is obtained by enzymolysis of additives including potato protein powder and black soldier fly powder through composite enzyme.

2. The additive for preparing pig feed according to claim 1, characterized in that: The additive for preparing pig feed is obtained by enzymolysis of additives including potato protein powder, black soldier fly powder and cinnamon essential oil through composite enzyme.

3. The additive for preparing pig feed according to claim 2, characterized in that: The complex enzyme is a mixture of alkaline protease, trypsin, lipase, xylanase and beta-glucanase.

4. The additive for preparing pig feed according to claim 3, characterized in that: The mass ratio of the potato protein powder, the black soldier fly powder and the cinnamon essential oil is (8-14): (2-4):

1.

5. The additive for preparing pig feed according to claim 4, characterized in that: The mass ratio of the alkaline protease, trypsin, lipase, xylanase and β-glucanase is (8-12): (8-12): (2-4): 1:

1.

6. The additive for preparing pig feed according to claim 3, characterized in that: The method for preparing the additive for pig feed comprises the following steps: S1. First, add 10-20 g of potato protein powder, black soldier fly powder and cinnamon essential oil to 40-60 ml of deionized water and mix well to obtain an enzymatic hydrolysis preparatory solution; S2, add 0.4-0.6g of composite protease to the preparatory solution for enzymolysis, stir and mix evenly, seal with plastic wrap, and perform enzymolysis in a water bath at 50-70°C for 3-5h. After the enzymolysis is completed, place the enzymolysis solution at 90-100°C for 10-20min to inactivate the enzyme, terminate the reaction, and obtain an enzymolysis product; S3. Cool the enzymatic hydrolysis product to room temperature, then centrifuge it at 8-12°C and 4000-6000 rpm for 8-12 minutes, and take the supernatant to obtain an additive for preparing pig feed.

7. A pig feed, characterized in that: The invention comprises the following raw materials in parts by weight: 70-80 parts of basic feed, 6-14 parts of the additive for preparing pig feed according to any one of claims 1-6, 20-30 parts of fermented feed and 0.020-0.025 parts of vitamin premix, wherein the fermented feed is prepared by solid-state fermentation method using the basic feed as a substrate and composite probiotics as a fermentation strain.

8. The pig feed according to claim 7, characterized in that: The composite probiotics are a mixture of plant lactobacillus, yeast and bacillus subtilis.

9. The pig feed according to claim 8, characterized in that: The mass ratio of the plant lactobacillus, yeast and bacillus subtilis is (1-3):1:(0.4-0.8).

10. The pig feed according to claim 7, characterized in that: In fermented feed, the inoculation amount of the strain is 0.04%-0.10%, the fermentation moisture is 40%-50%, the fermentation temperature is 30-50℃, and the fermentation time is 1-2d.