Preparation method of American ginseng beverage for enhancing immunity
Through the synergistic effect of American ginseng extract with modified sea cucumber powder, modified plant powder and other ingredients, an American ginseng drink that significantly improves immunity was prepared, which solved the problems of single ingredients, incomplete nutrition and difficult absorption of effective ingredients in the existing technology, and achieved more efficient immune enhancement and better taste.
Patent Information
- Application Number
- CN202510041032.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-10
- Publication Date
- 2025-04-04
AI Technical Summary
The existing beverage preparation technology for improving immunity has problems such as single ingredients, incomplete nutrition, difficult absorption of effective ingredients, and poor taste, which makes it difficult to effectively improve immunity.
American ginseng extract is used as the main body, combined with modified sea cucumber powder, modified plant powder and other auxiliary materials, and synergistically acts to prepare American ginseng drinks through enzymatic extraction, sonication and leaching.
It significantly improves the effect of improving immunity, has higher content of active ingredients, better taste, and significantly improves immunity after drinking.
Smart Images
Figure SMS_1
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of immune drinks, and specifically relates to a preparation method of an immunity-enhancing American ginseng drink. Background Art
[0002] Immunity is the body's own defense mechanism. It is the body's ability to resist external pathogenic factors and maintain the stability of the internal environment. In other words, the human immune system protects itself to maintain normal growth, development and health. Traditional Chinese medicine believes that the occurrence and development of immune diseases are mainly related to congenital and acquired factors. Congenital factors include insufficient innate endowment, and acquired factors include exogenous six evils, endogenous five evils, imbalance of Yingwei, disharmony of Qi and blood, and dysfunction of internal organs. Traditional Chinese medicine takes strengthening the body as the basic principle of treating diseases, and improves the patient's immune function and immunity level by adjusting the yin and yang balance of Qi and blood in the five internal organs.
[0003] American ginseng (Panax quinquefolius L) is a plant of the genus Panax in the Araliaceae family. It is native to Canada and the United States in North America and is an important medicinal plant. It tastes sweet and slightly bitter, and is mainly cool in nature. It has the functions of clearing away heat and relieving dryness, nourishing yin and replenishing qi. American ginseng is rich in ginsenosides, polysaccharides, amino acids, phenols, polyacetylenes, inorganic elements and volatile components. At present, the existing technology for preparing beverages to improve immunity mainly has the problems of single ingredients and incomplete nutrition. After long-term use, the function of the immune system is not enhanced; the taste is bad and it is difficult to stick to it, and the effective ingredients that improve immunity are not easily absorbed by the body. Summary of the invention
[0004] The purpose of the present invention is to provide a method for preparing an immunity-enhancing American ginseng beverage. By using American ginseng extract as the main body, supplemented with modified sea cucumber powder, modified plant powder and other auxiliary materials, the components work synergistically, and the obtained American ginseng beverage has a significant effect on improving immunity.
[0005] In order to achieve the above object, the present invention provides the following technical solutions: A method for preparing an immunity-enhancing American ginseng beverage comprises the following steps: S1: crushing, enzymolysis, standing and filtering American ginseng to obtain liquid A; S2: preparing modified sea cucumber powder and modified plant powder; S3: extracting 10-30 parts by weight of modified sea cucumber powder, 15-25 parts by weight of modified plant powder, 8-12 parts by weight of Dendrobium officinale, 3-7 parts by weight of Polygonatum sibiricum, 2-6 parts by weight of Dioscorea opposita, 4-8 parts by weight of Ginseng, 5-10 parts by weight of Poria cocos and 2-7 parts by weight of Lycium barbarum in an aqueous solution to obtain Liquid B; S4: ultrasonically treating the liquid A obtained in step S1, the liquid B obtained in step S2, and 3-8 parts of a 5-10% by mass white sugar aqueous solution to obtain a liquid C; S5: The liquid C obtained in step S3 is sterilized and packaged to obtain the American ginseng beverage.
[0006] Furthermore, the American ginseng in step S1 is crushed into particles of 80-100 mesh; American ginseng, water and enzyme are mixed in a mass ratio of 20:60:80:0.1-0.5, and enzymolysis is carried out at a temperature of 35-55°C and a pH of 4-4.5 for 6-10 hours to obtain an enzymolysis solution.
[0007] Furthermore, the enzymes are pectinase, cellulase and protease, with 10000U / g of cellulase, 10000U / g of protease and 8000U / g of pectinase, and the mass ratio of the three being 1:3-5:2-3.
[0008] Furthermore, the preparation process of the modified sea cucumber powder described in step S2 is: 10-20 parts by weight of fresh sea cucumbers are dissected and washed to remove mud and debris, then placed in 95-105°C water for 5-20s, taken out, minced, and added with 60-80 parts of room temperature water to obtain sea cucumber slurry; then 3-5 parts of Ganoderma lucidum powder are added thereto, ultrasonically treated at a power of 400-500w for 3-6min, and the modified sea cucumber powder is obtained after drying.
[0009] Furthermore, the preparation process of the modified plant powder described in step S2 is: by weight, 10-20 parts of figs, 5-10 parts of leek seeds and 3-8 parts of onion seeds are mixed and extracted in an ethanol solution; then 3-7 parts of oyster meat and 1-3 parts of oyster shells are added, and ultrasonic treatment is performed at a power of 800-900W for 5-8 minutes.
[0010] Furthermore, the extraction process is: adding 70-90 parts of ethanol solution and leaching at a temperature of 80-90° C. for 40-60 minutes.
[0011] Furthermore, the leaching process in step S3 is: adding 100-150 parts of water and leaching at a temperature of 60-80° C. for 30-60 minutes.
[0012] Furthermore, the parameters of the ultrasonic treatment in step S4 are: temperature 30-50° C., power 500-800 W, and time 5-10 min.
[0013] Furthermore, step S4 also contains 2-5 parts of vitamin B1 and 3-7 parts of vitamin B2 by weight.
[0014] Furthermore, in the sterilization process in step S5, the sterilization temperature is set at 115-125° C., the sterilization time is 30-40 min, and the sterilization pressure is maintained at 0.10-0.14 MPa.
[0015] Compared with the prior art, the advantages and beneficial effects of the present invention are: The present invention uses pectinase, cellulase and protease to perform combined enzymatic hydrolysis and extraction on American ginseng, which can greatly improve the acquisition rate of American ginseng active ingredients and improve the content of effective ingredients; sea cucumber pulp is prepared by a physical method to retain its functional ingredients to the greatest extent, and ganoderma lucidum powder is added to further enhance the immune ability; figs, leek seeds, onion seeds, oyster meat and oyster shells are mixed and ultrasonically treated to obtain modified plant powder, which has both animal and plant effective ingredients; in addition, under the joint action of ingredients such as vitamin B1, vitamin B2 and crystal sugar, the taste of the beverage is better, and after drinking, the immune ability is effectively improved. DETAILED DESCRIPTION
[0016] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0017] The sources of raw materials used in the present invention are as follows: American ginseng was from Xi'an Tianyi Biotechnology Co., Ltd., item number 100; pectinase, cellulase, and protease were from Shanghai Yuanye Biotechnology Co., Ltd., item numbers S10041, S10041, and S51641.
[0018] Example 1 This embodiment provides a method for preparing an immunity-enhancing American ginseng beverage, comprising the following steps: S1: crushing American ginseng into particles of 80-100 mesh; mixing American ginseng, water and enzyme in a mass ratio of 20:60:0.1, wherein the enzyme is composed of pectinase, cellulase and protease in a mass ratio of 1:3:2, and then performing enzymolysis reaction at a temperature of 35°C and a pH of 4 for 6 hours to obtain an enzymolysis solution; S2: Take 10g of fresh sea cucumber, dissect and wash to remove mud and debris, then put it into 95℃ water for 5s, take it out, mince it and add 60g of room temperature water to obtain sea cucumber slurry; then add 3g of Ganoderma lucidum powder, ultrasonically treat it at a power of 400W for 3min, and dry it at 80℃ to obtain modified sea cucumber powder; 10g figs, 5g leek seeds and 3g onion seeds were mixed, and then 70g ethanol solution was added, and the mixture was extracted at 80°C for 40min; then 3g oyster meat and 1g oyster shell were added, and the mixture was ultrasonically treated at a power of 800W for 8min to obtain modified plant powder; S3: Take 10g of modified sea cucumber powder, 15g of modified plant powder, 8g of Dendrobium officinale, 3g of Polygonatum sibiricum, 2g of Dioscorea opposita, 4g of Ginseng, 5g of Poria cocos, and 2g of Lycium barbarum, add 100g of water, and extract at 60°C for 30min to obtain liquid B; S4: treating the liquid A obtained in step S1, the liquid B obtained in step S2, 3 g of 5% by mass white sugar aqueous solution, 2 g of vitamin B1, and 3 g of vitamin B2 at a temperature of 30° C. and a power of 500 W for 5 min to obtain liquid C; S5: sterilize the liquid C obtained in step S3, control the sterilization temperature to 115° C., the sterilization time to 30 min, and maintain the sterilization pressure to 0.10 MPa; then package and process to obtain the American ginseng beverage.
[0019] Example 2 This embodiment provides a method for preparing an immunity-enhancing American ginseng beverage, comprising the following steps: S1: crushing American ginseng into particles of 80-100 mesh; mixing American ginseng, water and enzyme in a mass ratio of 20:80:0.5, wherein the enzyme is composed of pectinase, cellulase and protease in a mass ratio of 1:5:3, and then performing enzymolysis reaction at a temperature of 55°C and a pH of 4.5 for 10 hours to obtain an enzymolysis solution; S2: Take 20g of fresh sea cucumber, wash and remove mud and debris, then put it into 105℃ water for 20s, take it out, mince it and add 80g of room temperature water to obtain sea cucumber slurry; then add 5g of Ganoderma lucidum powder, treat it with ultrasound at a power of 500W for 6min, and dry it at 80℃ to obtain modified sea cucumber powder; 20g of figs, 10g of leek seeds and 8g of onion seeds were mixed, and then 90g of ethanol solution was added, and the mixture was extracted at 90°C for 60min; then 7g of oyster meat and 3g of oyster shell were added, and ultrasonic treatment was performed at a power of 900W for 8min to obtain modified plant powder; S3: Take 30g of modified sea cucumber powder, 25g of modified plant powder, 12g of Dendrobium officinale, 7g of Polygonatum sibiricum, 6g of Dioscorea opposita, 8g of Ginseng, 10g of Poria cocos, and 7g of Lycium barbarum, add 150g of water, and extract at 80°C for 60min to obtain liquid B; S4: treating the liquid A obtained in step S1, the liquid B obtained in step S2, 8 g of 10% aqueous solution of white granulated sugar, 5 g of vitamin B1, and 7 g of vitamin B2 by ultrasound at a temperature of 50° C. and a power of 800 W for 10 min to obtain liquid C; S5: sterilize the liquid C obtained in step S3, control the sterilization temperature to 125° C., the sterilization time to 40 min, and maintain the sterilization pressure to 0.14 MPa; then package and process to obtain the American ginseng beverage.
[0020] Example 3 This embodiment provides a method for preparing an immunity-enhancing American ginseng beverage, comprising the following steps: S1: crushing American ginseng into particles of 80-100 mesh; mixing American ginseng, water and enzyme in a mass ratio of 20:70:0.3, wherein the enzyme is composed of pectinase, cellulase and protease in a mass ratio of 1:4:3, and then performing enzymolysis reaction at a temperature of 45°C and a pH of 4.3 for 8 hours to obtain an enzymolysis solution; S2: Take 15g of fresh sea cucumber, dissect and wash to remove mud and debris, then put it into 100℃ water for 12s, take it out, mince it and add 70g of room temperature water to obtain sea cucumber slurry; then add 4g of Ganoderma lucidum powder, ultrasonically treat it at a power of 450W for 5min, and dry it to obtain modified sea cucumber powder; 15g figs, 7g leek seeds and 6g onion seeds were mixed, and then 85g ethanol solution was added, and the mixture was extracted at 85°C for 50min; then 6g oyster meat and 2g oyster shell were added, and the mixture was ultrasonically treated at a power of 850W for 7min to obtain modified plant powder; S3: Take 23g of modified sea cucumber powder, 18g of modified plant powder, 10g of Dendrobium officinale, 5g of Polygonatum sibiricum, 4g of Dioscorea opposita, 6g of Ginseng, 8g of Poria cocos, and 5g of Lycium barbarum, add 120g of water, and extract at 70°C for 45min to obtain liquid B; S4: Liquid A obtained in step S1, liquid B obtained in step S2, 6 g of 8% by mass white sugar aqueous solution, 4 g of vitamin B1, and 5 g of vitamin B2 are ultrasonically treated at a temperature of 40° C. and a power of 650 W for 7 min to obtain liquid C; S5: sterilize the liquid C obtained in step S3, control the sterilization temperature to 120° C., the sterilization time to 35 min, and maintain the sterilization pressure to 0.12 MPa; then package and process to obtain the American ginseng beverage.
[0021] Comparative Example 1 The difference between this comparative example and Example 1 is that pectinase is not added in step S1, and the other steps are the same.
[0022] Comparative Example 2 The difference between this comparative example and Example 1 is that no Ganoderma lucidum powder is added in step S2, and the other steps are the same.
[0023] Comparative Example 3 The difference between this comparative example and Example 1 is that step S2 is missing, modified sea cucumber powder and modified plant powder are not added, and the other steps are the same.
[0024] Comparative Example 4 The difference between this comparative example and Example 1 is that in step S2, no oyster meat and oyster shell are added to the modified plant powder, and the other steps are the same.
[0025] Comparative Example 5 The difference between this comparative example and Example 1 is that Dendrobium officinale and Polygonatum sibiricum are not added in step S3, and the other steps are the same.
[0026] Comparative Example 6 The difference between this comparative example and Example 1 is that the sterilization temperature in step S5 is 140° C. and the sterilization time is 50 min. The other steps are the same.
[0027] Immunity test: Experimental animals: nude mice, half male and half female, weighing 18-22g. Experimental grouping: The mice were divided into 8 groups, each group had 10 mice (half male and half female), and were fed with normal saline (control group), American ginseng beverage obtained in each embodiment and comparative example, respectively. Feeding method: The mice were gavaged with normal saline or American ginseng beverage 0.5ml / 20g body weight every day, once a day, for 2 consecutive weeks. Experimental items and detection indicators: The phagocytic index of mouse immune organs was detected by the experiment of mouse peritoneal macrophages phagocytizing chicken red blood cells; the thickening of the ear shell was detected by the mouse dinitrofluorobenzene-induced mouse DTH ear swelling test. The results are shown in Table 1.
[0028] Table 1 Performance test results
[0029] Note: Compared with the control group, p<0.05. From the above performance test results, it can be seen that Examples 1-3 have good phagocytosis percentage and phagocytosis index; in particular, the comprehensive performance of Example 3 is the most outstanding. However, since Comparative Examples 1-6 do not adopt the necessary technical solutions, their corresponding performance tests are significantly worse than those of the Examples. The above experimental results further prove the importance of the technical solutions defined in the present invention for its technical effects.
[0030] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. A method for preparing an immunity-enhancing American ginseng beverage, characterized in that: The steps include: S1: crushing, enzymolysis, standing and filtering American ginseng to obtain liquid A; S2: preparing modified sea cucumber powder and modified plant powder; S3: extracting 10-30 parts by weight of modified sea cucumber powder, 15-25 parts by weight of modified plant powder, 8-12 parts by weight of Dendrobium officinale, 3-7 parts by weight of Polygonatum sibiricum, 2-6 parts by weight of Dioscorea opposita, 4-8 parts by weight of Ginseng, 5-10 parts by weight of Poria cocos and 2-7 parts by weight of Lycium barbarum in an aqueous solution to obtain Liquid B; S4: ultrasonically treating the liquid A obtained in step S1, the liquid B obtained in step S2, and 3-8 parts of a 5-10% by mass white sugar aqueous solution to obtain a liquid C; S5: The liquid C obtained in step S3 is sterilized and packaged to obtain the American ginseng beverage.
2. The method for preparing an immunity-enhancing American ginseng beverage according to claim 1, characterized in that: The American ginseng in step S1 is crushed into particles of 80-100 mesh; American ginseng, water and enzyme are mixed according to a mass ratio of 20:60:80:0.1-0.5, and enzymolysis reaction is carried out at a temperature of 35-55° C. and a pH of 4-4.5 for 6-10 hours to obtain an enzymolysis solution.
3. The method for preparing an immunity-enhancing American ginseng beverage according to claim 2, characterized in that: The enzymes are pectinase, cellulase and protease, with 10000U / g of cellulase, 10000U / g of protease and 8000U / g of pectinase, and the mass ratio of the three being 1:3-5:2-3.
4. The method for preparing an immunity-enhancing American ginseng beverage according to claim 1, characterized in that: The preparation process of the modified sea cucumber powder described in step S2 is as follows: 10-20 parts by weight of fresh sea cucumbers are dissected and washed to remove mud and debris, then placed in 95-105°C water for 5-20s, taken out, minced, and added with 60-80 parts of room temperature water to obtain sea cucumber slurry; then 3-5 parts of Ganoderma lucidum powder are added thereto, ultrasonically treated at a power of 400-500w for 3-6min, and the modified sea cucumber powder is obtained after drying.
5. The method for preparing an immunity-enhancing American ginseng beverage according to claim 1, characterized in that: The preparation process of the modified plant powder in step S2 is as follows: by weight, 10-20 parts of figs, 5-10 parts of leek seeds and 3-8 parts of onion seeds are mixed and then extracted in an ethanol solution; then 3-7 parts of oyster meat and 1-3 parts of oyster shells are added, and ultrasonic treatment is performed at a power of 800-900W for 5-8 minutes.
6. The method for preparing an immunity-enhancing American ginseng beverage according to claim 5, characterized in that: The extraction process is: add 70-90 parts of ethanol solution and extract at a temperature of 80-90°C for 40-60 minutes.
7. The method for preparing an immunity-enhancing American ginseng beverage according to claim 1, characterized in that: The extraction process in step S3 is: adding 100-150 parts of water and leaching at a temperature of 60-80° C. for 30-60 minutes.
8. The method for preparing an immunity-enhancing American ginseng beverage according to claim 1, characterized in that: The parameters of the ultrasonic treatment in step S4 are: temperature 30-50° C., power 500-800 W, and time 5-10 min.
9. The method for preparing an immunity-enhancing American ginseng beverage according to claim 1, characterized in that: Step S4 also contains 2-5 parts of vitamin B1 and 3-7 parts of vitamin B2 by weight.
10. The method for preparing an immunity-enhancing American ginseng beverage according to claim 1, characterized in that During the sterilization process in step S5, the sterilization temperature is set at 115-125° C., the sterilization time is 30-40 min, and the sterilization pressure is maintained at 0.10-0.14 MPa.