A method for producing high-quality coconut flakes using coconut flake fermentation residual liquid

Abstract

The present invention discloses a method for producing high-quality coconut fruit by using residual liquor from coconut fruit fermentation. The method of the present invention comprises the following steps: (1) filtering the residual liquor from coconut fruit fermentation, and then adsorbing and decolorizing it through an anion exchange resin to obtain a recovered liquid; (2) adding coconut milk, inorganic acid, and sterile water to the recovered liquid to obtain a regeneration culture medium; (3) inoculating a docile Bacillus foetida seed liquid into the regeneration culture medium, and culturing it statically to obtain high-quality coconut fruit. The present invention reduces the production cost of coconut fruit by recovering the residual liquor from coconut fruit fermentation, optimizes the residual liquor treatment method, and improves the production quality of coconut fruit.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial fermentation, and particularly relates to a method for producing high-quality coconut flakes by utilizing coconut flake fermentation residual liquid. Background Art

[0002] The fermentation liquid obtained after the fermentation of coconut flakes contains a large amount of nutrients (sugars, inorganic acids, inorganic salts). If it is not recycled, the manufacturing cost of coconut flakes will increase. At the same time, discharging the above substances into wastewater will cause a great burden on the environmental protection.

[0003] At present, there are few methods for recycling the residual liquid from coconut fruit fermentation. Chinese invention patent CN201110436880.3 discloses a method for directly recycling the residual liquid from coconut fruit fermentation without treatment; the residual liquid recovery rate is only 10%-20%, and antioxidants need to be added to improve the fermentation effect, the overall cost reduction is not obvious, and a large amount of fermentation liquid is still discarded. Chinese invention patent CN201010113232.X discloses a method for recycling the residual liquid from coconut water fermentation, which is a simple treatment of precipitation and filtration of the fermentation residual liquid, used to prepare a regeneration culture medium, and the culture medium is sterilized at high temperature and fermented after cooling. The above two methods are simple and easy to operate, but the composition of the fermentation residual liquid is complex, and the coconut fruit produced may have many quality problems, such as soft texture of coconut fruit and yellow fruit. In addition, Chinese invention patent CN200910071848.2 discloses a method for purifying organic acids in fermentation residual liquid using bipolar membrane electrodialysis technology. This method purifies nutrients such as organic acids in fermentation residual liquid and recycles them for reuse. It is necessary to construct an anode chamber, a cathode chamber, and a plurality of separation chambers and concentration chambers constructed by partitions, bipolar membranes, and anion exchange membranes, and the equipment investment is relatively large; and the current efficiency of the electrodialysis separation process is only about 60%, the energy consumption is relatively high, and the processing cost is high, which is not conducive to industrial application.

[0004] Therefore, it is of great significance to develop a new method for producing high-quality coconut products using coconut fermentation residue. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a method for producing high-quality coconut nut using residual liquor from coconut nut fermentation. The present invention reduces the production cost of coconut nut by recycling the residual liquor from coconut nut fermentation, optimizes the residual liquor treatment method, and improves the production quality of coconut nut.

[0006] The technical solution of the present invention is as follows:

[0007] A method for producing high-quality coconut flakes using coconut flake fermentation residual liquid, the method comprising the following steps:

[0008] (1) filtering the coconut fruit fermentation residual liquid, and then adsorbing and decolorizing through an anion exchange resin to obtain a recovered liquid;

[0009] (2) adding coconut milk, mineral acid and sterile water to the recovered liquid to obtain a regeneration medium;

[0010] (3) Inoculate the seed liquid of Bacillus docileus into the regeneration medium, culture it statically, and obtain high-quality coconut fruit.

[0011] Furthermore, in step (1), the method for obtaining the coconut fruit fermentation residual liquid is: performing solid-liquid separation on the coconut fruit produced by natural fermentation, and collecting the separated liquid; compressing the separated coconut fruit to obtain a compressed liquid; combining the compressed liquid and the separated liquid, filtering through a sieve, collecting the filtrate for centrifugal treatment, and collecting the supernatant to obtain the coconut fruit fermentation residual liquid.

[0012] Furthermore, the compression multiple is 5-10 times; the mesh size of the sieve is 4-20 meshes; the centrifugal speed is 5000rpm-8000rpm, and the time is 5min-10min.

[0013] Furthermore, in step (1), the filtration includes one or both of microfiltration and ultrafiltration.

[0014] Furthermore, the microfiltration membrane is selected from any one of an organic filter membrane and an inorganic filter membrane; the material of the organic filter membrane is selected from one of polyethylene, polyethersulfone, and polyvinylidene fluoride; the material of the inorganic filter membrane is selected from any one of porous ceramics and porous glass; the pore size of the microfiltration membrane is 0.1μm-0.45μm; the inlet pressure of the microfiltration is ≤0.2MPa, the outlet pressure is ≤0.2MPa, and the circulating liquid temperature is controlled to 25℃-40℃.

[0015] Furthermore, the ultrafiltration membrane is an organic membrane, the material of which is selected from any one of cellulose acetate, polyethersulfone, and polypropylene, and the ultrafiltration treatment step comprises using an ultrafiltration membrane with a molecular weight cutoff of 10KD-100KD to ultrafilter the coconut fermentation residual liquid; the inlet pressure of the ultrafiltration is ≤0.6MPa, the outlet pressure is ≤0.6MPa, and the circulating material temperature is controlled to be 25°C-40°C.

[0016] Furthermore, in step (1), the anion exchange resin is composed of styrene, acrylate, and chlorobenzene as monomers, cross-linked by divinylbenzene, and the functional group is an amine group; the adsorption decolorization loading rate is 2BV / h-5BV / h; the particle size of the anion exchange resin is 0.4mm-1.7mm, the water content is 40%-60%, the pore size is 1nm-200nm, the porosity is 20%-60%, and the wet true density is 1.02g / cm 3 -1.10g / cm 3 .

[0017] Furthermore, in step (2), the formula of the regeneration culture medium is composed of: 500mL-900mL of recovery liquid, 0g-20g of sugar, 15g-20g of coconut milk, 6mL-10mL of inorganic acid, and the rest is sterile water per liter of culture medium.

[0018] Further, in step (3), after the regeneration medium is inoculated with the seed solution of Bacillus docile, the bacterial concentration in the regeneration medium is 10 6 cfu / mL-10 7 cfu / mL.

[0019] Furthermore, in step (3), the culture temperature is 28°C-32°C and the culture time is 7 days-10 days.

[0020] The beneficial technical effects of the present invention are:

[0021] The present invention provides a method for producing high-quality coconut fruit by using coconut fruit fermentation residual liquid, and obtains a recovery liquid mainly composed of sugar substances by solid-liquid separation, membrane separation, anion exchange resin adsorption decolorization and removal of anions. In the present invention, microfiltration is used to remove the residual bacteria, fine fibers and other insoluble particles in the fermentation residual liquid, or microfiltration-ultrafiltration treatment is used to further remove macromolecular compounds (proteins, nucleic acid polymers, enzymes, etc.) in the fermentation residual liquid, thereby purifying the fermentation liquid; anion exchange resin can adsorb colored substances in the fermentation residual liquid, and at the same time remove anions such as chloride ions and sulfate ions that are not conducive to fermentation, so that the substances in the recovery liquid are mainly sugar and water. Compared with directly using the untreated fermentation recovery liquid, the coconut fruit fermentation residual liquid is treated by the method of the present invention, which solves the problem that the whiteness and hardness of the coconut fruit produced are not good due to the high content of insoluble solids and metabolites in the coconut fruit fermentation residual liquid and the darker color, so that the coconut fruit yield is significantly improved, and the whiteness and hardness are significantly improved.

[0022] The present invention provides a method for finely treating fermentation residual liquid, which not only reduces waste liquid discharge, but also greatly reduces production costs. Membrane separation technology is used to remove insoluble substances, macromolecular proteins and metabolites in fermentation recovery liquid; anion exchange resin is used to remove colored substances and anions in fermentation recovery liquid, and small molecular nutrients such as sugar are recovered and reused; the recovered residual liquid obtained by this method is used to produce coconut fruit, which not only reduces the existing raw material cost by 40-50%, but also the coconut fruit yield can reach 85%, and the coconut fruit quality is also greatly improved. This residual liquid recovery method is conducive to industrial large-scale production and has significant economic and social benefits. DETAILED DESCRIPTION

[0023] The present invention will be described in detail below in conjunction with the embodiments.

[0024] The anion exchange resin used in the following examples of the present invention was purchased from Zhejiang Zhengguang Industrial Co., Ltd., with the product number D354FD.

[0025] The Komagataeibacter oboediens JGG1 used in the present invention was collected by ourselves, the strain identification has been completed, and it has been deposited in the China Center for Type Culture Collection with the deposit number M 20221774 and the deposit date of 2022-11-11.

[0026] Example 1

[0027] A method for producing high-quality coconut flakes by utilizing coconut flake fermentation residual liquid comprises the following steps:

[0028] (1) The coconut fruit produced by natural fermentation is subjected to solid-liquid separation, and large insoluble particles are removed to obtain a separated liquid; the separated coconut fruit is compressed 5 times to obtain a compressed liquid; the compressed liquid and the separated liquid are combined, and then filtered through a 20-mesh stainless steel filter, the filtrate is collected, and centrifuged at 5000 rpm for 5 minutes, and the supernatant is collected to obtain a coconut fruit fermentation residual liquid. Subsequently, microfiltration is performed through a microfiltration membrane separation system, the filter membrane is made of polyethersulfone, the pore size is 0.1 μm, the inlet pressure is 0.2 MPa, the outlet pressure is 0.2 MPa, and the circulating liquid temperature is controlled at 25-40°C. The filtrate after microfiltration treatment is loaded onto the column and flows from top to bottom into the column filled with anion exchange resin at a flow rate of 3BV / h, with a processing volume of 10BV and a temperature of 30-40°C. The effluent is collected. When the transmittance of the effluent is 85-90% of the sample solution and the conductivity reaches 1000-1200μs / cm, the injection is stopped and the effluent is collected, i.e., the recovered solution.

[0029] (2) Take 900 mL of the recovered liquid, 17 g of coconut milk, and 6 mL of inorganic acid to obtain a regeneration medium.

[0030] (3) Inoculate the regeneration medium with Bacillus docileus seed solution, and measure the bacterial density in the medium to be 10 6 cfu / mL, and cultured at 30°C for 8 days to obtain high-quality coconut fruit with a coconut fruit yield of 76%, a hardness of 144N, and a whiteness of 88.5%.

[0031] Example 2

[0032] A method for producing high-quality coconut flakes by utilizing coconut flake fermentation residual liquid comprises the following steps:

[0033] (1) The coconut fruit produced by natural fermentation is subjected to solid-liquid separation, and large insoluble particles are removed to obtain a separated liquid; the separated coconut fruit is compressed 8 times to obtain a compressed liquid; the compressed liquid is combined with the separated liquid, and then filtered through a 20-mesh stainless steel filter, the filtrate is collected, and centrifuged at 5000rpm for 5min, and the supernatant is collected to obtain a coconut fruit fermentation residual liquid. Subsequently, microfiltration is performed through a membrane separation system, the filter membrane material is polyethersulfone, the pore size is 0.2μm, the inlet pressure is 0.2MPa, the outlet pressure is 0.2MPa, and the circulating liquid temperature is controlled at 25°C-40°C. The filtrate after microfiltration is ultrafiltered through an organic membrane ultrafiltration system, the filter membrane material is polyethersulfone, the filter membrane has a molecular weight cutoff of 100KD, the inlet pressure is 0.6Mpa, the outlet pressure is 0.6Mpa, and the circulating material temperature is controlled at 25°C-40°C. The filtrate after ultrafiltration treatment is loaded onto the column and flows from top to bottom into a column filled with anion exchange resin at a flow rate of 3BV / h, with a processing volume of 10BV and a temperature of 30℃-40℃. The effluent is collected. When the transmittance of the effluent is 85%-90% of the injection liquid and the conductivity reaches 1000μs / cm-1200μs / cm, the injection is stopped and the effluent is collected, i.e., the recovered liquid.

[0034] (2) Take 900 mL of the recovered liquid, 17 g of coconut milk, and 6 mL of inorganic acid to obtain a regeneration medium.

[0035] (3) Inoculate the regeneration medium with Bacillus docileus seed solution, and measure the bacterial density in the medium to be 10 6 cfu / mL, and cultured at 30°C for 8 days to obtain high-quality coconut fruit with a coconut fruit yield of 85%, a hardness of 146N, and a whiteness of 90.2%.

[0036] Example 3

[0037] A method for producing high-quality coconut flakes by utilizing coconut flake fermentation residual liquid comprises the following steps:

[0038] (1) The coconut fruit produced by natural fermentation is subjected to solid-liquid separation, and large insoluble particles are removed to obtain a separated liquid; the separated coconut fruit is compressed 8 times to obtain a compressed liquid; the compressed liquid is combined with the separated liquid, and then filtered through a 20-mesh stainless steel filter, the filtrate is collected, and centrifuged at 5000rpm for 5min, and the supernatant is collected to obtain a coconut fruit fermentation residual liquid. Subsequently, microfiltration is performed through a membrane separation system, the filter membrane material is polyethersulfone, the pore size is 0.45μm, the inlet pressure is 0.2MPa, the outlet pressure is 0.2MPa, and the circulating liquid temperature is controlled at 25℃-40℃. The filtrate after microfiltration is ultrafiltered through an organic membrane ultrafiltration system, the filter membrane material is polyethersulfone, the filter membrane has a molecular weight cutoff of 100KD, the inlet pressure is 0.6Mpa, the outlet pressure is 0.6Mpa, and the circulating material temperature is controlled at 25℃-40℃. The filtrate after ultrafiltration treatment is loaded onto the column and flows from top to bottom into a column filled with anion exchange resin at a flow rate of 3BV / h, with a processing volume of 10BV and a temperature of 30℃-40℃. The effluent is collected. When the transmittance of the effluent is 85%-90% of the injection liquid and the conductivity reaches 1000μs / cm-1200μs / cm, the injection is stopped and the effluent is collected, i.e., the recovered liquid.

[0039] (2) Take 900 mL of the recovered liquid, 17 g of coconut milk, and 6 mL of inorganic acid to obtain a regeneration medium.

[0040] (3) Inoculate the regeneration medium with Bacillus docileus seed solution, and measure the bacterial density in the medium to be 10 6 cfu / mL, and cultured at 30°C for 8 days to obtain high-quality coconut fruit with a coconut fruit yield of 83%, a hardness of 145N, and a whiteness of 90.0%.

[0041] Example 4

[0042] A method for producing high-quality coconut flakes by utilizing coconut flake fermentation residual liquid comprises the following steps:

[0043] (1) The coconut fruit produced by natural fermentation is subjected to solid-liquid separation, and large insoluble particles are removed to obtain a separated liquid; the separated coconut fruit is compressed 8 times to obtain a compressed liquid; the compressed liquid is combined with the separated liquid, and then filtered through a 20-mesh stainless steel filter, the filtrate is collected, and centrifuged at 5000rpm for 5min, and the supernatant is collected to obtain a coconut fruit fermentation residual liquid. Subsequently, microfiltration is performed through a membrane separation system, the filter membrane material is polyethersulfone, the pore size is 0.1μm, the inlet pressure is 0.2MPa, the outlet pressure is 0.2MPa, and the circulating liquid temperature is controlled at 25℃-40℃. The filtrate after microfiltration is ultrafiltered through an organic membrane ultrafiltration system, the filter membrane material is polyethersulfone, the filter membrane has a molecular weight cutoff of 20KD, the inlet pressure is 0.6Mpa, the outlet pressure is 0.6Mpa, and the circulating material temperature is controlled at 25℃-40℃. The filtrate after ultrafiltration treatment is loaded onto the column and flows from top to bottom into a column filled with anion exchange resin at a flow rate of 3BV / h, with a processing volume of 10BV and a temperature of 30℃-40℃. The effluent is collected. When the transmittance of the effluent is 85%-90% of the injection liquid and the conductivity reaches 1000μs / cm-1200μs / cm, the injection is stopped and the effluent is collected, i.e., the recovered liquid.

[0044] (2) Take 900 mL of the recovered liquid, 17 g of coconut milk, and 6 mL of inorganic acid to obtain a regeneration medium.

[0045] (3) Inoculate the regeneration medium with Bacillus docileus seed solution, and measure the bacterial density in the medium to be 10 6 cfu / mL, and cultured at 30°C for 8 days to obtain high-quality coconut fruit with a coconut fruit yield of 85.5%, a hardness of 150N, and a whiteness of 90.4%.

[0046] Comparative Example 1

[0047] A method for producing coconut fruit comprises the following steps:

[0048] (1) Prepare a fermentation medium by taking 30 g of sucrose, 17 g of coconut milk, 6 mL of inorganic acid, and 870 mL of sterile water.

[0049] (2) Inoculate the fermentation medium with Bacillus docile seed solution, and measure the bacterial density in the medium to be 10 6 cfu / mL, and cultured at 30°C for 8 days to obtain coconut fruit with a coconut fruit yield of 75%, a hardness of 152N, and a whiteness of 90.5%.

[0050] Comparative Example 2

[0051] A method for producing coconut flakes by utilizing coconut flake fermentation residual liquid comprises the following steps:

[0052] (1) performing solid-liquid separation on coconut fruit produced by natural fermentation, removing large insoluble particles, and obtaining a separated liquid; the separated coconut fruit is compressed by 5 times to obtain a compressed liquid; the compressed liquid and the separated liquid are combined, filtered through a 20-mesh stainless steel filter, the filtrate is collected, centrifuged at 5000 rpm for 5 minutes, and the supernatant is collected to obtain a coconut fruit fermentation residual liquid.

[0053] (2) Take 900 mL of coconut fruit fermentation residual liquid, 17 g of coconut milk, and 6 mL of inorganic acid to obtain a regeneration culture medium.

[0054] (3) Inoculate the regeneration medium with Bacillus docileus seed solution, and measure the bacterial density in the medium to be 10 6 cfu / mL, and cultured at 30°C for 8 days to obtain coconut fruit with a coconut fruit yield of 50%, a hardness of 113N, and a whiteness of 65.8%.

[0055] Comparative Example 3

[0056] A method for producing coconut flakes by utilizing coconut flake fermentation residual liquid comprises the following steps:

[0057] (1) performing solid-liquid separation on coconut fruit produced by natural fermentation, removing large insoluble particles, and obtaining a separated liquid; compressing the separated coconut fruit by 8 times to obtain a compressed liquid; combining the compressed liquid and the separated liquid, filtering through a 20-mesh stainless steel filter, collecting the filtrate, centrifuging at 5000 rpm for 5 minutes, collecting the supernatant, and obtaining a coconut fruit fermentation residual liquid; sterilizing the coconut fruit fermentation residual liquid at 100° C. for 20 minutes, and cooling the room temperature to room temperature to obtain a sterilized coconut fruit fermentation residual liquid.

[0058] (2) 900 mL of sterilized coconut fermentation residue, 17 g of coconut milk, and 6 mL of inorganic acid were taken to obtain a regeneration medium.

[0059] (3) Inoculate the regeneration medium with Bacillus docileus seed solution, and measure the bacterial density in the medium to be 10 6 cfu / mL, and cultured at 30°C for 8 days to obtain coconut fruit with a coconut fruit yield of 60%, a hardness of 98N, and a whiteness of 55.7%.

[0060] Comparative Example 4

[0061] A method for producing coconut flakes by utilizing coconut flake fermentation residual liquid comprises the following steps:

[0062] (1) The coconut fruit produced by natural fermentation is subjected to solid-liquid separation, and large insoluble particles are removed to obtain a separated liquid; the separated coconut fruit is compressed by 6 times to obtain a compressed liquid; the compressed liquid is combined with the separated liquid, and then filtered through a 10-mesh stainless steel filter, the filtrate is collected, and centrifuged at 5000rpm for 5min, and the supernatant is collected to obtain the coconut fruit fermentation residual liquid. Subsequently, microfiltration is performed through a membrane separation system, the filter membrane material is polyethersulfone, the pore size is 0.1μm, the inlet pressure is 0.2MPa, the outlet pressure is 0.2MPa, and the circulating liquid temperature is controlled to 25℃-40℃. The filtrate after microfiltration is ultrafiltered through an organic membrane ultrafiltration system, the filter membrane has a molecular weight cutoff of 100kD, the filter membrane material is polyethersulfone, the inlet pressure is 0.6Mpa, the outlet pressure is 0.6Mpa, the circulating material temperature is controlled to 25℃-40℃, and the ultrafiltrate is collected, i.e., the recovered liquid.

[0063] (2) Take 900 mL of the recovered liquid, 17 g of coconut milk, and 6 mL of inorganic acid to obtain a regeneration medium.

[0064] (3) Inoculate the regeneration medium with Bacillus docileus seed solution, and measure the bacterial density in the medium to be 10 6 cfu / mL, and cultured at 30°C for 8 days to obtain coconut fruit with a coconut fruit yield of 68%, a hardness of 126N, and a whiteness of 66.3%.

[0065] Test Case

[0066] (1) Coconut quality evaluation

[0067] The coconut flakes quality evaluation indexes of the present invention include coconut flakes yield, whiteness and hardness.

[0068] Coconut yield: refers to the ratio of the wet weight of coconut flakes to the volume of fermentation liquid.

[0069] Hardness determination method: SMS TA-XT PLUS physical property analyzer TPA mode was used to determine the hardness of coconut fruit. The P / 35 probe was selected and the coconut fruit to be tested was cut into 2cm×2cm size. Two extrusion tests were carried out on the texture analyzer with a test speed of 1mm / s, a compression ratio of 75%, an initial force of 5g, and the tested coconut fruit was fermented original fruit slices.

[0070] Whiteness determination method: Use a whiteness meter to determine the whiteness of coconut fruit. Cut the coconut fruit to be tested into round pieces that fit the mold. After calibrating with white and brown modules, measure the whiteness value. The higher the whiteness value, the closer it is to the chromaticity of the white module.

[0071] The coconut fruits prepared in Examples 1-4 of the present invention and Comparative Examples 1-4 were evaluated, and the results are shown in Table 1.

[0072] Table 1 Coconut quality evaluation

[0073]

[0074] As can be seen from the results in Table 1, the high-quality coconut fruit prepared by Examples 1-4 of the present invention is significantly improved in whiteness, yield, and hardness compared to Comparative Example 2, indicating that the present invention further removes residual bacteria, suspended solids, macromolecular proteases and other metabolites in the fermentation residual liquid by filtering (microfiltration or microfiltration-ultrafiltration treatment) and adsorption decolorization of the coconut fruit fermentation residual liquid after solid-liquid separation, which is beneficial to the fermentation production of coconut fruit. Compared with Comparative Example 3, in Comparative Example 3, the yield of the coconut fruit obtained by high-temperature sterilization of the coconut fruit fermentation residual liquid after solid-liquid separation is improved compared to Comparative Example 2, but the coconut fruit is darker in color and has a lower hardness, which may be due to the destruction of nutrients by the high-temperature sterilization process, which deteriorates the quality of coconut fruit. In addition, it can be seen from the results of Comparative Example 4 that it is necessary to add the step of anion resin adsorption decolorization to treat the coconut fruit fermentation residue in the present invention. The pigment in the fermentation residue is removed by adsorption decolorization, and at the same time, the anions (such as chloride ions, sulfate ions, etc.) in the fermentation residue are exchanged to remove the anions that are not conducive to fermentation, and a recovered liquid with sugar and water as the main components is obtained, thereby preparing a regeneration culture medium for the fermentation culture of Bacillus docile, which can effectively increase the coconut fruit yield and improve the whiteness.

[0075] (2) Conductivity evaluation before and after adsorption decolorization

[0076] The filtrate after microfiltration treatment and the effluent after resin adsorption decolorization in step (1) of Example 1 of the present invention, and the filtrate after ultrafiltration treatment and the effluent after resin adsorption decolorization in step (1) of Examples 2-4 were taken, and the conductivity was tested by a conductivity meter. The results are shown in Table 2.

[0077]

[0078]

[0079] From the results in Table 2, it can be seen that after resin adsorption, the conductivity dropped from 2200-2230 μs / cm to 1000-1200 μs / cm, which can effectively remove anions.

[0080] The above is only a preferred embodiment of the present invention, and the present invention is not limited to the above embodiments. It is understood that other improvements and changes directly derived or associated by those skilled in the art without departing from the spirit and concept of the present invention should be considered to be included in the protection scope of the present invention.

Claims

1. A method for producing high-quality coconut flakes using coconut flake fermentation residual liquid, characterized in that: The method comprises the following steps: (1) filtering the coconut fruit fermentation residual liquid, and then adsorbing and decolorizing through an anion exchange resin to obtain a recovered liquid; (2) adding coconut milk, mineral acid and sterile water to the recovered liquid to obtain a regeneration medium; (3) Inoculate the seed liquid of Bacillus docileus into the regeneration medium, culture it statically, and obtain high-quality coconut fruit.

2. The method according to claim 1, characterized in that In step (1), the method for obtaining the coconut fruit fermentation residual liquid is: performing solid-liquid separation on the coconut fruit produced by natural fermentation, and collecting the separated liquid; The separated coconut fruit is compressed to obtain a compressed liquid; The compressed liquid and the separated liquid are combined, filtered through a sieve, the filtrate is collected for centrifugal treatment, and the supernatant is collected to obtain the coconut fruit fermentation residual liquid.

3. The method according to claim 2, characterized in that The compression multiple is 5 to 10 times; the mesh size of the sieve is 4 to 20 meshes; the centrifugal speed is 5000 rpm to 8000 rpm, and the time is 5 min to 10 min.

4. The method according to claim 1, characterized in that: In step (1), the filtration includes one or both of microfiltration and ultrafiltration.

5. The method according to claim 4, characterized in that The microfiltration membrane is selected from any one of an organic membrane and an inorganic membrane; the material of the organic membrane is selected from one of polyethylene, polyethersulfone, and polyvinylidene fluoride; the material of the inorganic membrane is selected from any one of porous ceramics and porous glass; the pore size of the microfiltration membrane is 0.1μm-0.45μm; the inlet pressure of the microfiltration is ≤0.2MPa, the outlet pressure is ≤0.2MPa, and the circulating liquid temperature is controlled to be 25℃-40℃.

6. The method according to claim 4, characterized in that The ultrafiltration membrane is an organic membrane, the material of which is selected from any one of cellulose acetate, polyethersulfone, and polypropylene. The ultrafiltration treatment step comprises using an ultrafiltration membrane with a molecular weight cutoff of 10KD-100KD to ultrafilter the coconut fermentation residual liquid; the inlet pressure of the ultrafiltration is ≤0.6MPa, the outlet pressure is ≤0.6MPa, and the circulating material temperature is controlled to be 25°C-40°C.

7. The method according to claim 1, characterized in that In step (1), the anion exchange resin is composed of styrene, acrylate and chlorobenzene as monomers, cross-linked by divinylbenzene, and the functional group is an amine group; the adsorption decolorization loading rate is 2BV / h-5BV / h; the particle size of the anion exchange resin is 0.4mm-1.7mm, the water content is 40%-60%, the pore size is 1nm-200nm, the porosity is 20%-60%, and the wet true density is 1.02g / cm 3 -1.10g / cm 3 .

8. The method according to claim 1, characterized in that In step (2), the formula of the regeneration culture medium is composed of: 500mL-900mL of recovery liquid, 0g-20g of sugar, 15g-20g of coconut milk, 6mL-10mL of inorganic acid, and the rest is sterile water per liter of culture medium.

9. The method according to claim 1, characterized in that: In step (3), after the regeneration medium is inoculated with the seed solution of Bacillus docile, the bacterial concentration in the regeneration medium is 10 6 cfu / mL-10 7 cfu / mL.

10. The method according to claim 1, characterized in that In step (3), the culture temperature is 28°C-32°C and the culture time is 7 days-10 days.

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