Spatial array electrode and method for snuff bulb monk cap cell layer nerve electrophysiological signals

Abstract

The invention discloses a space array electrode and a method for collecting an olfactory bulb monk cap cell layer nerve electrophysiological signal, in the space array electrode, an electrode beam is used for being implanted into a rat olfactory bulb, the electrode beam comprises a plurality of electrode single beams, each electrode single beam is composed of a plurality of electrode wires, and the electrode wires are arranged in the space array electrode. The spatial height between the electrode wires is arranged according to the position of a monk cap cell layer in the olfactory bulb area; the polyimide tube wraps the electrode single beam to restrain the electrode wires of the electrode single beam, so that the electrode wires are kept parallel to each other; one end of the PCB interface is connected with the electrode beam, and the other end is used for being connected with a connector of external acquisition equipment.

Description

Technical Field

[0001] The invention relates to the technical field of spatial array electrodes, in particular to a spatial array electrode and a method for collecting neuroelectrophysiological signals of the mitral cell layer of the olfactory bulb. Background Art

[0002] The olfactory perception system, as one of the most important perception methods for rats to explore the world, is an important brain area for neuroscientists to study how external odor molecules are converted into neural signals and to identify and classify odors. Among them, the olfactory bulb is the core part of the olfactory perception system, and the mitral cell layer of the olfactory bulb is the key area for olfactory signal processing and encoding. The mitral cells in the olfactory bulb participate in the tuning and integration of odor signals through complex synaptic connections. Obtaining more high-quality neuroelectrophysiological signals from the mitral cell layer will help scientists to deeply understand the processing mechanism of olfactory information. However, the structure of the mitral cell layer in the olfactory bulb is a spherical ribbon structure with a thickness of only 100 μm, but its function is very important in the processing and transmission of olfactory signals. The small thickness makes it very difficult and challenging to collect electrical signals in this area.

[0003] At present, the electrodes used for neuroelectrophysiological acquisition on the market mainly include microwire array electrodes, silicon-based electrodes, and various flexible electrodes. However, using these electrodes to collect neural electrical signals from the mitral cell layer of the olfactory bulb is not a good choice. For example, usually, most microwire array electrodes are two-dimensional planar array electrodes, and there is no spatial array arrangement. They can only collect neural electrical signals from the dorsal or ventral unilateral mitral cell layer, and cannot obtain comprehensive mitral cell neural electrical signal activity information. In addition to microwire array electrodes, high-channel, high-density silicon-based electrodes and flexible electrodes are also widely used to collect neural electrophysiological signals. However, high-density channels are only linearly and equidistantly arranged in space. After implantation in the olfactory bulb, most of the channels will fall into other non-mitral cell layers such as the granular cell layer. The number of channels that can actually collect mitral cell layer signals is relatively small, resulting in most of the channels being wasted.

[0004] The information disclosed in this Background section is only for enhancement of understanding of the background of the invention and therefore it may contain information that does not form the prior art that is already known to a person of ordinary skill in the art. Summary of the invention

[0005] In view of the shortcomings or defects of the prior art, a spatial array electrode and method for collecting neuroelectrophysiological signals of the mitral cell layer of the olfactory bulb are provided, which can maximize the utilization of all collection channels and simultaneously collect neuroelectrophysiological signals of the dorsal and ventral mitral cell layers of the olfactory bulb.

[0006] The purpose of the present invention is achieved through the following technical solutions.

[0007] A spatial array electrode for collecting electrophysiological signals of nerves in the mitral cell layer of the olfactory bulb comprises:

[0008] An electrode bundle, wherein the electrode bundle comprises a plurality of electrode bundles, wherein the electrode bundles are composed of a plurality of electrode wires, and the space height between the electrode wires is arranged according to the position of the mitral cell layer in the olfactory bulb region;

[0009] A polyimide tube wrapping the electrode bundle to constrain the electrode wires so that the electrode wires remain parallel to each other;

[0010] The interface has one end connected to the electrode bundle and the other end used to connect to the connector of the external collection device.

[0011] In the spatial array electrode for collecting electrophysiological signals of the olfactory bulb mitral cell layer nerves, the electrode bundle is provided with 16 polyimide tubes, and the 16 polyimide tubes are arranged in a 4×4 array. Each polyimide tube contains 4 electrode wires, and there are a total of 4×4×4 electrode microwires to collect 64-channel electrical signals.

[0012] In the spatial array electrode for collecting neurophysiological signals of the olfactory bulb mitral cell layer, the diameter of the polyimide tube is 0.15 mm.

[0013] In the spatial array electrode for collecting neurophysiological signals of the olfactory bulb mitral cell layer, four electrode wires are arranged as follows: the four electrode wires are divided into two layers, each layer contains two electrode wires, the spacing between the two layers is 3.5 mm, and there is a floating distance of 100 μm between the two electrode wires in the upper or lower layer.

[0014] In the spatial array electrode for collecting neurophysiological signals of the olfactory bulb mitral cell layer, the electrode wire has a diameter of 35 μm, is made of nickel-chromium alloy, is covered with an acetimide insulating layer, and has exposed metal tip contacts.

[0015] In the spatial array electrode for collecting neurophysiological signals of the olfactory bulb mitral cell layer, the interface is divided into a J1 surface and a J2 surface, and each surface is distributed with 32 channels of signal collection circuits.

[0016] In the spatial array electrode for collecting neuroelectrophysiological signals of the olfactory bulb mitral cell layer, an insulating layer is provided on the surface of the PCB interface.

[0017] In the spatial array electrode for collecting neurophysiological signals of the olfactory bulb mitral cell layer, the insulating layer is formed by covering with AB glue.

[0018] The method for preparing a spatial array electrode for collecting neurophysiological signals of the mitral cell layer of the olfactory bulb comprises the following steps:

[0019] Cut a polyimide tube and cut 4 electrode wires, two of which are 30 mm long and the other two are 25 mm long. Fix the polyimide tube on a smooth horizontal surface, insert the 4 electrode wires into the polyimide tube, then pull the contact segments of the 4 electrode wires to 5 mm, and keep the tips of the 4 contact segments flush. Use tweezers to hold the two 25 mm electrode wires at 5 mm, adjust the contact spacing of the two electrode wires to 100 μm, and then use tweezers to pull the two 30 mm electrode wires back to 1.5 mm, adjust the contact spacing of the two electrode wires to 100 μm. After the spatial arrangement of the 4 electrode wires is adjusted, dip the glue into the entrance of the polyimide tube to fix the spatial arrangement distance of the four electrode wires to form a single electrode bundle.

[0020] After the electrode bundle with 16 electrode bundles arranged in space is completed, the 16 electrode bundles are divided into 8 groups, each group has two electrode bundles, and glue is applied to the polyimide tube to make the two electrode bundles bonded together in parallel. The 8 groups of electrode bundles are further bonded together in pairs to form four groups, each group contains 4 electrode bundles, and the 4 groups of electrode bundles are stacked up in 4 layers in sequence, and glue is applied to the polyimide tube to keep the 4×4 spatial array fixed to form a motor bundle;

[0021] After scraping off the insulation layer of the top connection section of the 64-conductor electrode wire, use glue to fix the upper polyimide tube to the blank space in the upper half of the notch on the PCB substrate, and fill the remaining lower end to the notch. The 16 polyimide tubes are divided into 8 on the J1 surface and 8 on the J2 surface. Among them, all 25 mm long electrode wire connection sections of the 8 polyimide tubes on the J1 surface are welded to the pad contacts on the left side of the J1 surface, and the 30 mm long electrode wire connection sections are welded to the pad contacts on the right side of the J1 surface.

[0022] In the method described, AB glue covers the pads on the J1 and J2 surfaces, and forms a hard protective layer after the AB glue solidifies.

[0023] Compared with the prior art, the present invention has the following beneficial effects: the spatial array electrode of the present invention can simultaneously collect the neurophysiological signals of the dorsal and ventral mitral cell layers of the rat olfactory bulb, and can realize the flexibility of collecting signals of the mitral cell layer of the rat olfactory bulb. It makes up for the shortcomings of the existing electrodes that cannot obtain comprehensive mitral cell neural electrical signal activity information, and most of the channels are located in other non-mitral cell layers such as the granular cell layer.

[0024] The above description is only an overview of the technical solution of the present invention. In order to make the technical means of the present invention clearer and to achieve the extent that those skilled in the art can implement it according to the contents of the specification, and in order to make the above and other purposes, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are exemplified below. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] By reading the detailed description of the preferred specific embodiments below, various other advantages and benefits of the present invention will become clear to those of ordinary skill in the art. The drawings in the specification are only for the purpose of illustrating the preferred embodiments and are not considered to be limitations of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained based on these drawings without creative work. Moreover, the same reference numerals are used to represent the same components throughout the drawings.

[0026] In the attached picture:

[0027] Figure 1 A schematic diagram of the structure of a spatial array electrode for collecting neurophysiological signals of the mitral cell layer of the olfactory bulb provided by one embodiment of the present invention;

[0028] Figure 2 A physical diagram of the overall structure of a spatial array electrode for collecting neurophysiological signals of the mitral cell layer of the olfactory bulb provided in one embodiment of the present invention;

[0029] Figure 3 A schematic diagram of the overall spatial structure and size of a spatial array electrode for collecting neurophysiological signals of the mitral cell layer of the olfactory bulb provided in one embodiment of the present invention;

[0030] Figure 4 A schematic diagram of a spatial 3D arrangement of a 4-conductor single-bundle spatial array electrode for collecting neurophysiological signals of the mitral cell layer of the olfactory bulb provided by an embodiment of the present invention;

[0031] Figure 5 A 3D schematic diagram of a 4×4×4 spatial arrangement of 16 electrode bundles of a spatial array electrode for collecting neurophysiological signals of the mitral cell layer of the olfactory bulb provided by an embodiment of the present invention;

[0032] Figure 6 A schematic diagram of the structure of the PCB interfaces J1 and J2 of the spatial array electrode for collecting neurophysiological signals of the mitral cell layer of the olfactory bulb provided by one embodiment of the present invention;

[0033] Figure 7 A schematic diagram of neural electrical signals collected by a spatial array electrode for collecting neural electrophysiological signals of the mitral cell layer of the olfactory bulb provided in one embodiment of the present invention, including 64-channel local field potentials and representative neural pulse signals.

[0034] The present invention is further explained below in conjunction with the accompanying drawings and embodiments. DETAILED DESCRIPTION

[0035] The specific embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although the specific embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided in order to enable a more thorough understanding of the present invention and to enable the scope of the present invention to be fully communicated to those skilled in the art.

[0036] It should be noted that certain words are used in the specification and claims to refer to specific components. Those skilled in the art should understand that technicians may use different nouns to refer to the same component. This specification and claims do not use the difference in nouns as a way to distinguish components, but use the difference in the functions of the components as the criterion for distinction. As mentioned throughout the specification and claims, "including" or "comprising" is an open term, so it should be interpreted as "including but not limited to". The subsequent description of the specification is a preferred embodiment of the present invention, but the description is based on the general principles of the specification and is not intended to limit the scope of the present invention. The scope of protection of the present invention shall be determined by the attached claims.

[0037] To facilitate understanding of the embodiments of the present invention, several specific embodiments will be further explained below with reference to the accompanying drawings, and each of the accompanying drawings does not constitute a limitation on the embodiments of the present invention.

[0038] For a better understanding, Figures 1 to 7 As shown, a spatial array electrode for collecting electrophysiological signals of nerves in the mitral cell layer of the olfactory bulb includes:

[0039] An electrode bundle can be implanted in a suitable object such as a rat olfactory bulb, wherein the electrode bundle includes a plurality of electrode bundles, the electrode bundles are composed of a plurality of electrode wires, and the space height between the electrode wires is arranged according to the location of the mitral cell layer in the olfactory bulb region; specifically, the dorsal and ventral mitral cell layers of the rat olfactory bulb are about 3.5 mm apart, and the 4 leads are divided into 2 layers, upper and lower, at intervals of 3.5 mm, and the lower layer corresponds to the ventral mitral cell layer, and the maximum positioning distance of the electrode contacts is 4.1-4.2 mm. The overall length of the electrode is 5 mm, and the positions of the upper two electrode contacts are 1.5 mm away from the electrode starting point. When the electrode is implanted in the rat olfactory bulb, the lower electrode contacts are positioned at 4.1-4.2 mm, and the upper two electrode contacts will fall at 600-800 μm in the olfactory bulb, corresponding to the location of the dorsal mitral cell layer. Furthermore, due to individual and tissue differences, the positions of the dorsal and ventral mitral cell layers will fluctuate to a certain extent. In order to maximize the contact with the mitral cell layer, the two electrode contacts in the two layers will be separated by a floating distance of 100 μm.

[0040] A polyimide tube wrapping the electrode bundle to constrain the electrode wires thereof so that the electrode wires remain parallel to each other;

[0041] PCB interface, one end of which is connected to the electrode bundle, and the other end is used to connect to the connector of the external collection device.

[0042] In a preferred embodiment of the spatial array electrode for collecting electrophysiological signals of the olfactory bulb mitral cell layer nerves, the electrode bundle is provided with 16 polyimide tubes, and the 16 polyimide tubes are arranged in a 4×4 array. Each polyimide tube contains 4 electrode wires, and there are a total of 4×4×4 electrode wires to collect 64-channel electrical signals.

[0043] In a preferred embodiment of the spatial array electrode for collecting electrophysiological signals of nerves in the mitral cell layer of the olfactory bulb, the diameter of the polyimide tube is 0.15 mm.

[0044] In a preferred embodiment of the spatial array electrode for collecting neural electrophysiological signals of the olfactory bulb mitral cell layer, the four electrode wires are arranged as follows: the four electrode wires are divided into two layers, each layer contains two electrode wires, the spacing between the two layers is 3.5 mm, and there is a floating distance of 100 μm between the two electrode wires in the upper or lower layer.

[0045] In a preferred embodiment of the spatial array electrode for collecting neurophysiological signals from the mitral cell layer of the olfactory bulb, the electrode wire has a diameter of 35 μm, is made of nickel-chromium alloy, is covered with an acetylimide insulating layer, and has exposed metal tip contacts.

[0046] In the preferred embodiment of the spatial array electrode for collecting neurophysiological signals of the olfactory bulb mitral cell layer, the PCB interface is divided into a J1 surface and a J2 surface, and each surface is distributed with 32 channels of signal collection circuits.

[0047] In a preferred embodiment of the spatial array electrode for collecting neurophysiological signals of the olfactory bulb mitral cell layer, an insulating layer is provided on the surface of the PCB interface.

[0048] In a preferred embodiment of the spatial array electrode for collecting neurophysiological signals of the olfactory bulb mitral cell layer, the insulating layer is formed by covering with AB glue.

[0049] The method for preparing a spatial array electrode for collecting neurophysiological signals of the mitral cell layer of the olfactory bulb comprises the following steps:

[0050] Cut a polyimide tube and cut 4 electrode wires, two of which are 30 mm long and the other two are 25 mm long. Fix the polyimide tube on a smooth horizontal surface, insert the 4 electrode wires into the polyimide tube, then pull the contact segments of the 4 electrode wires to 5 mm, and keep the tips of the 4 contact segments flush. Use tweezers to hold the two 25 mm electrode wires at 5 mm, adjust the contact spacing of the two electrode wires to 100 μm, and then use tweezers to pull the two 30 mm electrode wires back to 1.5 mm, adjust the contact spacing of the two electrode wires to 100 μm. After the spatial arrangement of the 4 electrode wires is adjusted, dip the glue into the entrance of the polyimide tube to fix the spatial arrangement distance of the four electrode wires to form a single electrode bundle.

[0051] After the electrode bundle with 16 electrode bundles arranged in space is completed, the 16 electrode bundles are divided into 8 groups, each group has two electrode bundles, and glue is applied to the polyimide tube to make the two electrode bundles bonded together in parallel. The 8 groups of electrode bundles are further bonded together in pairs to form four groups, each group contains 4 electrode bundles, and the 4 groups of electrode bundles are stacked up in 4 layers in sequence, and glue is applied to the polyimide tube to keep the 4×4 spatial array fixed to form a motor bundle;

[0052] After scraping off the insulation layer of the top connection section of the 64-conductor electrode wire, use glue to fix the upper polyimide tube to the blank space in the upper half of the notch on the PCB substrate, and fill the remaining lower end to the notch. The 16 polyimide tubes are divided into 8 on the J1 surface and 8 on the J2 surface. Among them, all 25 mm long electrode wire connection sections of the 8 polyimide tubes on the J1 surface are welded to the pad contacts on the left side of the J1 surface, and the 30 mm long electrode wire connection sections are welded to the pad contacts on the right side of the J1 surface.

[0053] In a preferred embodiment of the method, AB glue covers the pads on the J1 and J2 surfaces, and forms a hard protective layer after the AB glue solidifies.

[0054] In one embodiment, the spatial structure and size of the spatial array electrodes for collecting the neurophysiological signals of the mitral cell layer of the olfactory bulb are determined based on the rat brain localization map. Figure 3According to the sixth edition of the rat brain localization atlas, the olfactory bulb brain localization position is 16.08 mm between the ears and 7.08 mm from the bregma (AP: 7.08), which is the second page of the brain localization atlas. A 1 mm × 1 mm rectangular window is opened 0.6 mm from the sagittal suture. According to the brain atlas, the dorsal mitral cell layer of the olfactory bulb is approximately 600-700 μm below the dura mater, and the maximum distance between the electrode contacts of the two dorsal leads is located at 700 μm below the dura mater. Due to individual differences and tissue differences, the position of the dorsal mitral cell layer will fluctuate to a certain extent. In order to maximize the contact with the dorsal mitral cell layer, the distance between the two dorsal electrode contacts will be 100 μm.

[0055] The ventral mitral cell layer is about 4.2 mm, and the maximum positioning distance of the two ventral electrode contacts is 4.1-4.2 mm. Due to individual differences and tissue differences, the position of the ventral mitral cell layer will fluctuate to a certain extent. In order to maximize the contact with the ventral mitral cell layer, the distance between the two ventral electrode contacts will be 100 μm.

[0056] Based on the fact that the dorsal and ventral mitral cell layers of the rat olfactory bulb are approximately 3.5 mm apart, the two dorsal electrode contacts and the two ventral electrode contacts are 3.5 mm apart.

[0057] The overall length of the electrode is 5 mm, and the position of the two dorsal electrode contacts is 1.5 mm from the electrode starting point. When implanted, the ventral electrode contacts are positioned at 4.1-4.2 mm, and the two dorsal electrode contacts will fall on the olfactory bulb at 600-800 μm, corresponding to the location of the dorsal mitral cell layer.

[0058] In the embodiments of this application, Figure 4 The arrangement of a single bundle of 4-channel electrode wires includes the following execution steps.

[0059] Specifically, a polyimide tube was cut, which was about 10 mm long and 0.15 mm in diameter, and four electrode wires were cut using special scissors, two of which were 30 mm long and the other two were 25 mm long.

[0060] Fix a separate polyimide tube on a smooth horizontal surface with medical tape. With the help of a high-power microscope and a standard scale, first insert the four electrode wires into the polyimide tube. Then pull the contact segments of the four electrode wires to 5 mm and keep the tips of the four contact segments flush.

[0061] After completing the above operations, use fine tweezers to adjust the contact distance between the two electrode wires of 25 mm at 5 mm to 100 μm.

[0062] After completing the above operations, use fine tweezers to pull the two 30 mm electrode wires back to 1.5 mm, and adjust the contact spacing of the two electrode wires to 100 μm according to design requirements.

[0063] After adjusting the spatial arrangement of the four electrode wires, use a toothpick to dip a small amount of glue and tap it lightly on the entrance of the polyimide tube to fix the spatial arrangement distance of the four electrode wires.

[0064] In the embodiments of this application, Figure 5 , the arrangement of 16 arrays includes the following execution steps.

[0065] Specifically, according to the above operation, after completing the 16 single-beam spatially arranged electrode clusters, in order to form a 4×4 array electrode, the 16 electrode clusters are first divided into 8 groups, each with two single beams, and a small amount of glue is applied to the polyimide tube to bond the two beams together in parallel. The 8 groups of electrode clusters continue to be bonded together in parallel, that is, divided into four groups, each containing 4 single-beam electrode clusters. The 4 groups of electrode clusters are stacked up in 4 layers in sequence, and a small amount of glue is applied to the polyimide tube to keep the 4×4 spatial array fixed.

[0066] In the embodiments of this application, Figure 6 , the welding method of the electrode and the PCB board includes the following steps.

[0067] Specifically, when the above steps are completed, the connection segment, polyimide and contact segment parts of the 4×4×4 spatial array electrode suitable for collecting neurophysiological signals of the mitral cell layer of the olfactory bulb of rats are completed.

[0068] The above-mentioned connecting section 64-conductor electrode wires are all coated with insulating functions. In order to achieve signal conduction, the insulating layer at the top of the connecting section needs to be scraped off with tweezers. After scraping off the insulating layer of the top connecting section of the 64-conductor electrode wire, the above-mentioned three parts that have been fixed are taken as a whole, and the upper polyimide tube is fixed to the blank space in the upper half of the gap at the PCB substrate in the figure below with glue, and the remaining lower end is filled to the gap, so that the three parts of the whole are fixed on the PCB substrate.

[0069] By using a welding gun and solder, the electrode wire with the insulation layer removed is welded to the pad of the PCB circuit board. The specific implementation method is to use a high-precision microscope to divide 16 polyimide tubes into 8 on the J1 surface and 8 on the J2 surface, wherein all 25 mm long electrode wire connection sections of the 8 polyimide tubes on the J1 surface are welded to the pad contacts on the left side of the J1 surface, and 30 mm long electrode wire connection sections are welded to the pad contacts on the right side of the J1 surface. After welding, the electrode wires on the pads are arranged, and two silver wires are welded to the pad contacts at the ground wire and reference on both sides of the J1 surface.

[0070] Similarly, all 25 mm long electrode wire connection sections of the 8 polyimide tubes on the J2 surface are welded to the pad contacts on the left side of the J2 surface, and 30 mm long electrode wire connection sections are welded to the pad contacts on the right side of the J2 surface.

[0071] After all the welding is completed, the 64-conductor electrode wires are arranged according to the arrangement, and the AB glue is covered on the pads of J1 and J2. After the AB glue solidifies, a hard protective layer is formed.

[0072] In the embodiments of the present application, the spatial array electrode use steps for collecting neuroelectrophysiological signals of the mitral cell layer of the olfactory bulb and the neuroelectrophysiological collection conditions include the following execution steps.

[0073] Specifically, rats weighing 250-290 g were selected, anesthetized with 4% isoflurane, the hair on the head of the rats was shaved, and the rats were immediately fixed on a stereotaxic apparatus, the anesthesia concentration was maintained at 1.5-2%, the gas flow rate was 500-700 ml / min, and finally erythromycin eye ointment was applied to the eyes of the rats to keep the eyes moist.

[0074] Clean the surgical opening with 75% alcohol, iodine, and 75% alcohol in that order. Use scissors to remove the skin and muscle tissue between the two ears and the two eyes of the rat's skull to fully expose the hard bone layer. After the skull surface is dried, obvious sagittal suture, anterior bregma, and posterior bregma will appear. At this time, use a clamp to vertically fix the skull drill, move the tip of the skull drill to the front end of the sagittal suture, and move it slowly backwards to ensure that the tip is always on the sagittal suture during the movement, and the front and back heights are close to the skull surface, proving that the rat's head fixation meets the positioning requirements.

[0075] According to the rat brain localization map, the location of the rat olfactory bulb was determined, which was 16.08 mm between the ears, 7.08 mm from the anterior bregma (AP: 7.08), and 0.6 mm from the sagittal suture (ML: 0.6). After setting the above parameters using an automatic brain localizer, a 1 mm × 1 mm rectangular window was opened in the left or right olfactory bulb with a skull drill, and the dura mater in the rectangular window was peeled off with precision forceps. A small hole was opened under the contralateral olfactory bulb as the implantation point of the reference electrode. Another small hole was opened in the brain area close to the olfactory bulb as the implantation point of the ground wire.

[0076] When using the spatial array electrode to collect the neurophysiological signals of the mitral cell layer of the rat olfactory bulb, the tip of the electrode is soaked in anhydrous ethanol and 75% alcohol in advance to clean and disinfect the tip of the electrode.

[0077] Fix the electrode base on the precision brain locator with an electrode holder, adjust the angle so that the direction of the electrode wire is perpendicular to the skull plane, connect it to the input end of the signal acquisition system, slowly move the electrode close to the cranial window, place the reference wire and ground wire of the electrode into the pre-drilled holes, and add special tissue glue to fix the ground wire and reference wire.

[0078] Clean the rectangular window positioned in the olfactory bulb, use a precise brain stereotaxic instrument to lower the tip of the entire electrode to the brain surface, set the spatial parameters of the brain locator to 0, and implant the tip of the electrode at a position 4.1-4.2 mm below the dura mater of the olfactory bulb (DV: 4.1-4.2 mm).

[0079] Use medical cotton balls to absorb a small amount of bleeding, and drip special tissue glue into the cranial window to initially fix the electrode array, and then use thinner dental cement to flow into the gap of the excess electrode array at the upper end to further fix the electrode array. Then drip the tissue glue on the entire surgical wound, wait for it to solidify, and seal the entire exposed skull and the upper end of the electrode with dental cement. After it is completely solidified, turn off the anesthesia, remove the rat from the positioning device, and place it on a heating pad until it recovers. After it can move independently, put it in a mouse cage and raise it alone.

[0080] After waiting for 2-3 days, it can be used to collect neural electrical signals. Figure 7 The local field potential recording of the olfactory bulb detected by the present application has high stability and high yield rate, and the detected neural pulse signal has a good waveform shape and a large signal-to-noise ratio.

[0081] The spatial array electrode for collecting neuroelectrophysiological signals of the mitral cell layer of the rat olfactory bulb can simultaneously collect neuroelectrophysiological signals of the dorsal and ventral mitral cell layers of the rat olfactory bulb, can realize the flexibility of collecting signals of the mitral cell layer of the rat olfactory bulb, can maximize the use of the number of channels, obtain a large number of neuronal information, and avoid the situation where most of the channels are wasted. This application makes up for the shortcomings of the electrodes currently on the market that cannot obtain comprehensive mitral cell neural electrical signal activity information, and most of the channels are located in other non-mitral cell layers such as the granular cell layer.

[0082] The basic principles of the present application are described above in conjunction with specific embodiments. However, it should be noted that the advantages, strengths, effects, etc. mentioned in the present application are only examples and not limitations, and it cannot be considered that these advantages, strengths, effects, etc. are required by each embodiment of the present application. In addition, the specific details disclosed above are only for the purpose of illustration and ease of understanding, not for limitation, and the above details do not limit the present application to being implemented by adopting the above specific details.

[0083] The above description has been given for the purpose of illustration and description. In addition, this description is not intended to limit the embodiments of the present application to the forms disclosed herein. Although multiple example aspects and embodiments have been discussed above, those skilled in the art will recognize certain variations, modifications, changes, additions and sub-combinations thereof.

Claims

1. A spatial array electrode for collecting neurophysiological signals of the mitral cell layer of the olfactory bulb, characterized in that: These include, An electrode bundle, wherein the electrode bundle comprises a plurality of electrode bundles, wherein the electrode bundles are composed of a plurality of electrode wires, and the space height between the electrode wires is arranged according to the position of the mitral cell layer in the olfactory bulb region; A polyimide tube wrapping the electrode bundle to constrain the electrode wires so that the electrode wires remain parallel to each other; The interface has one end connected to the electrode bundle and the other end used to connect to the connector of the external collection device.

2. The spatial array electrode for collecting electrophysiological signals of the olfactory bulb mitral cell layer as claimed in claim 1, characterized in that: Preferably, the electrode bundle is provided with 16 polyimide tubes.

3. The spatial array electrode for collecting electrophysiological signals of the olfactory bulb mitral cell layer as claimed in claim 1, characterized in that: The polyimide tube has a diameter of 0.15 mm.

4. The spatial array electrode for collecting electrophysiological signals of the olfactory bulb mitral cell layer as claimed in claim 1, characterized in that: The four electrode wires are arranged as follows: the four electrode wires are divided into two layers, each layer contains two electrode wires, the spacing between the two layers is 3.5 mm, and there is a floating distance of 100 μm between the two electrode wires in the upper or lower layer.

5. The spatial array electrode for collecting electrophysiological signals of the olfactory bulb mitral cell layer as claimed in claim 1, characterized in that: The electrode wire has a diameter of 35 μm and is made of nickel-chromium alloy, covered with an acetylimide insulation layer, with its metal tip contact exposed.

6. The spatial array electrode for collecting electrophysiological signals of the olfactory bulb mitral cell layer as claimed in claim 1, characterized in that: The interface is divided into a J1 surface and a J2 surface, and each surface is provided with 32 channels of signal acquisition circuits.

7. The spatial array electrode for collecting electrophysiological signals of the olfactory bulb mitral cell layer as claimed in claim 1, characterized in that: The interface surface is provided with an insulating layer.

8. The spatial array electrode for collecting electrophysiological signals of the olfactory bulb mitral cell layer as claimed in claim 7, characterized in that: The insulating layer is formed by covering with AB glue.

9. The method for preparing a spatial array electrode for collecting electrophysiological signals of nerves in the mitral cell layer of the olfactory bulb according to any one of claims 1 to 8.

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