A mild skin rejuvenation and effective acne-removing composition and its use

Abstract

The application discloses a kind of mild skin and efficient acne-removing composition and its application, it is related to cosmetic technical field.The composition of the application includes component A, component B and component C;The component A includes hydrolyzed Opuntia Ficus-indica flower extract and guaiac extract;The component B includes nucifera oil and Rosa Damascena flower water, and the component C is papain.The application will hydrolyzed Opuntia Ficus-indica flower extract, nucifera oil, Rosa Damascena flower water, papain and guaiac extract are combined to find synergistic effect, and these components are formed by the composition of specific compatibility It has the effects of keratolytic, regulating water-oil balance, protecting skin barrier and improving acne, especially applied to sensitive skin, can gently exfoliate, and the satisfaction of subject is high, and the acceptance is strong, and can be repeatedly and long-term used.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a composition for mild skin rejuvenation and efficient acne removal and application thereof. Background Art

[0002] Acne patients experience excessive keratinocyte proliferation, which causes the epithelial cell layer of the sebaceous gland duct to thicken, narrow in diameter, and decrease in patency. This ultimately leads to acute occlusion of the sebaceous gland duct, resulting in follicular swelling and the formation of comedones. This suppressed sebum secretion creates an anaerobic environment for Propionibacterium acnes, leading to secondary inflammatory acne. Electron microscopy reveals an increase in the number and size of keratinized granules and an accumulation of lipid droplets in keratinized areas.

[0003] In addition, Propionibacterium acnes and its cell wall components prompt keratinocytes to secrete IL-1, which leads to TLR-2 activation, which is the initial step of hyperkeratinization of the sebaceous glands of the hair follicles. TLR-2 can not only prompt keratinocytes to secrete IL-1, IL-6, IL-8 or TNF-α, but also promote macrophages to express IL-8 and IL-12, thereby causing hyperkeratosis, dry and flaky skin, and damaged skin barrier, which damages skin health.

[0004] Chemical exfoliation is a popular method nowadays. It involves applying acidic chemicals to the surface of the skin, causing controllable damage to the epidermis and / or dermis, removing old cuticles, and then promoting skin repair and regeneration. The type, concentration, and duration of action of acidic chemicals will affect the depth of chemical peeling. According to the depth of peeling, it can be divided into shallow, medium, and deep peeling. The deeper the peeling depth, the more obvious the effect, but the greater the possibility of adverse reactions. In addition, not all skin types are suitable for acid peeling. People with sensitive skin, thin stratum corneum, etc. need to be extra cautious. Improper use can trigger acne, make the skin barrier more fragile, and damage skin health. Therefore, seeking a product that can gently exfoliate and remove acne has become a technical problem that needs to be solved urgently in this field. Summary of the Invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a composition and its application for gentle skin rejuvenating and highly effective acne removal. The composition has the effects of gently exfoliating, regulating water-oil balance, protecting the skin barrier, and improving acne. It is especially suitable for use on sensitive skin, can gently exfoliate, has high subject satisfaction and strong acceptance, and can be used repeatedly and for a long time.

[0006] To achieve the above object, the technical solution adopted by the present invention is:

[0007] In a first aspect, the present invention provides a composition for gentle skin rejuvenating and highly effective acne removal, the composition comprising component A, component B and component C; component A comprises hydrolyzed prickly pear cactus flower extract and guaiac wood extract; component B comprises grapefruit seed oil and Rosa centifolia flower water; and component C is papain.

[0008] Hydrolyzed Opuntia Indica Flower Extract is a natural plant extract obtained from the flowers of the Opuntia Indica plant through a hydrolysis process. Its main active ingredients may include polysaccharides, proteins, amino acids, natural phenolic compounds, flavonoids, vitamin C, and minerals. Hydrolyzed Opuntia Indica Flower Extract is rich in polysaccharides and has strong moisturizing properties. Polysaccharides can bind to water molecules to form a hydration layer, thereby helping the skin retain moisture and enhancing its ability to retain moisture. Proteins and amino acids are the foundation of skin repair and renewal, providing the skin with essential nutrients and helping to strengthen the skin's barrier function.

[0009] Guaiacum officinale Extract is derived from Guaiacum officinale and its related species. Guaiacum is widely used in traditional medicine, especially in the treatment of skin diseases. Its extract is rich in bioactive ingredients, mainly including guaiacol and its derivatives, flavonoids and phenolic compounds. Guaiacum officinale extract is rich in natural moisturizing factors, which can provide long-lasting moisture to the skin, help maintain the water and oil balance of the skin, and have certain moisturizing and soothing effects; the active ingredients in guaiacum officinale extract can promote the renewal of skin cells, help repair damaged skin tissue, promote skin repair, improve skin roughness, make the skin smoother and more delicate, and improve skin texture.

[0010] Grapefruit is very rich in pharmacological effects and nutritional value. Grapefruit (CITRUS GRANDIS) seed oil is an essential oil extracted from grapefruit seeds. Grapefruit seed oil contains a large amount of terpene compounds, which have antioxidant, anti-inflammatory, antibacterial and sedative effects. Grapefruit seed oil also contains a small amount of flavonoids and vitamin C. These ingredients have antioxidant, anti-aging and skin repair effects.

[0011] Rosa damascena flower water is a natural plant water with a wide range of skin care benefits. It contains natural sugars, amino acids, and minerals that help maintain skin hydration and enhance its ability to retain moisture. It deeply nourishes the skin and keeps it soft and smooth. Amino acids also help repair damaged skin barrier and enhance its water retention function. It has good soothing and calming effects, making it suitable for sensitive skin. Its aromatic components, such as rose alcohol, have anti-inflammatory and calming effects, which can help reduce skin redness, irritation, and irritation. Rose alcohol and other aromatic compounds have natural antibacterial, antifungal, and antiviral effects, which can remove bacteria, fungi, and other microorganisms on the skin surface.

[0012] Papain is the main active ingredient in papaya, which is a cysteine protease enzyme. It can hydrolyze the peptide bonds of proteins, especially large molecular proteins. Papain can break down proteins in the stratum corneum on the skin surface, especially keratin in the stratum corneum. By breaking down these hardened keratin proteins into smaller fragments, papain helps remove dead skin cells and promotes skin surface renewal.

[0013] The present application found that when the above several plant extracts are used together, they can synergistically achieve the effects of exfoliating, regulating water and oil balance, protecting skin barrier, and improving acne, especially on sensitive skin. It can gently exfoliate, and the satisfaction of the subjects is high, and the acceptance is strong, and it can be used repeatedly and long-term.

[0014] As a preferred embodiment of the first aspect, the mass ratio of component A, component B and component C in the composition is component A: component B: component C = (1.1-6):(1.01-11):(0.01-0.1). Exemplarily, the mass ratio of component A, component B and component C in the composition can be any value or a range value between any values in 1.1:1.01:0.01, 1.1:1.5:0.05, 4:2:0.05, 5:4:0.05, 6:11:0.1.

[0015] As a preferred embodiment of the first aspect, the mass ratio of component A, component B and component C in the composition is component A: component B: component C = (1.5-4):(2.05-8.8):(0.04-0.08).

[0016] As a preferred embodiment of the first aspect, the mass ratio of component A, component B and component C in the composition is component A: component B: component C = 2:6.7:0.06.

[0017] The application researches and finds that the mass ratio of component A, component B and component C in the keratolytic and acne-removing composition will affect the scale index, the keratinocyte renewal ability and the inhibition ability of papules and comedones to some extent, thereby affecting the keratolytic and acne-removing effect of the composition, and will also affect the skin barrier repair ability and moisturizing effect of the composition. When the mass ratio of component A, component B and component C is (1.1-6):(1.01-11):(0.01-0.1), especially (0.5-4):(2.05-8.8):(0.04-0.08), the keratolytic and acne-removing composition obtained has more excellent effect on the keratinocyte renewal ability and the inhibition ability of papules and comedones, more greatly enhances the inhibition ability of the composition on the keratinocyte renewal ability and comedones, and improves the comprehensive ability of the composition in keratolytic, acne-removing, moisturizing and repairing. The research finds that when the mass ratio of component A, component B and component C is 2:6.7:0.06, the overall comprehensive effect of the composition of the application is best.

[0018] As a preferred embodiment of the first aspect, in the component A, the mass ratio of the hydrolyzed Opuntia ficus-indica flower extract and the guaiacwood extract is hydrolyzed Opuntia ficus-indica flower extract: guaiacwood extract = (0.1-3):(1-3). Exemplarily, the mass ratio of the hydrolyzed Opuntia ficus-indica flower extract and the guaiacwood extract can be any value or a range value between any values in 0.1:1, 0.1:1.5, 0.1:2, 1:1, 2:1, 3:1, 3:2 and 0.1:3.

[0019] As a preferred embodiment of the first aspect, in the component A, the mass ratio of the hydrolyzed Opuntia ficus-indica flower extract and the guaiacwood extract is hydrolyzed Opuntia ficus-indica flower extract: guaiacwood extract = (0.3-2):(1.2-2).

[0020] As a preferred embodiment of the first aspect, in the component A, the mass ratio of the hydrolyzed Opuntia ficus-indica flower extract and the guaiacwood extract is hydrolyzed Opuntia ficus-indica flower extract: guaiacwood extract = 0.5:1.5.

[0021] The application researches and finds that the mass ratio of the hydrolyzed Opuntia ficus-indica flower extract and the guaiacwood extract in the component A of the keratolytic and acne-removing composition will affect the TEWL value and the skin moisture content to some extent, thereby affecting the barrier repair and moisturizing ability of the composition, and when the mass ratio of the hydrolyzed Opuntia ficus-indica flower extract and the guaiacwood extract is (0.1-3):(1-3), especially (0.3-2):(1.2-2), the barrier repair and moisturizing ability of the composition can be improved, and the research finds that when the mass ratio of the hydrolyzed Opuntia ficus-indica flower extract and the guaiacwood extract is 0.5:1.5, the barrier repair and moisturizing ability of the component A of the application is best.

[0022] As a preferred embodiment of the first aspect, in component B, the mass ratio of grapefruit seed oil to Rosa centifolia floral water is grapefruit seed oil:Rosa centifolia floral water=(0.01-1):(1-10). For example, the mass ratio of grapefruit seed oil to Rosa centifolia floral water can be any value selected from 0.01:1, 0.01:2, 0.01:4, 0.01:6, 0.01:8, 0.01:10, 1:1, 1:4, 1:8, 1:10, or a range thereof.

[0023] As a preferred embodiment of the first aspect, in the component B, the mass ratio of grapefruit seed oil to Rosa centifolia flower water is grapefruit seed oil: Rosa centifolia flower water = (0.05-0.8): (2-8).

[0024] As a preferred embodiment of the first aspect, in the component B, the mass ratio of grapefruit seed oil to Rosa centifolia flower water is grapefruit seed oil:Rosa centifolia flower water=0.4:6.3.

[0025] The present invention has found that the mass ratio of grapefruit seed oil and Rosa centifolia flower water in component B of the exfoliating and acne-removing composition will affect the composition's effect on inhibiting Propionibacterium acnes and its sedative effect to a certain extent, thereby affecting the composition's acne-removing, anti-inflammatory, and antibacterial abilities. When the mass ratio of grapefruit seed oil to Rosa centifolia flower water is further selected to be (0.01-1):(1-10), especially (0.05-0.8):(2-8), the composition's acne-removing, anti-inflammatory, and antibacterial abilities can be enhanced. Studies have found that when the mass ratio of grapefruit seed oil to Rosa centifolia flower water is 0.4:6.3, the acne-removing, anti-inflammatory, and antibacterial effects of component B of the present invention are optimal.

[0026] As a preferred embodiment of the first aspect, the method for preparing grapefruit seed oil comprises the following steps:

[0027] (1) Take grapefruit seeds, dry them, crush them, add water and mix them in a water bath;

[0028] (2) adding protease to perform enzymolysis to obtain an enzymolysis product;

[0029] (3) centrifuging the enzymatic hydrolysate to break the emulsion, separating the emulsion layer, and centrifuging the emulsion layer to obtain free oil;

[0030] (4) drying the free oil to obtain the grapefruit seed oil.

[0031] As a preferred embodiment of the first aspect, the method for preparing grapefruit seed oil satisfies at least one of the following conditions:

[0032] (a) The material-liquid ratio of water added and mixed in step (1) is 1g: (4-8)ml; the water bath temperature is 80-90°C, and the time is 1-2h;

[0033] (b) The protease in step (2) is alkaline protease, the enzymatic hydrolysis temperature is 40-45°C, and the enzymatic hydrolysis time is 2-3h;

[0034] (c) The centrifugation conditions in step (3) are both 3000-5000 r / min and 10-20 min.

[0035] The present invention finds that different extraction methods have an impact on grapefruit seed oil, and affect the synergistic effect of grapefruit seed oil and other components, affecting the anti-inflammatory, antibacterial and sedative effects of the composition, thereby affecting the overall comprehensive effect of the composition.

[0036] In a second aspect, the present invention provides use of the mild skin rejuvenating and highly effective acne-removing composition as described in the first aspect in the preparation of cosmetics.

[0037] As a preferred embodiment of the second aspect, the cosmetics include one or more of lotion, emulsion, cream, mask, essence, and spray.

[0038] In a third aspect, the present invention provides a cosmetic comprising the exfoliating and anti-acne composition.

[0039] As a preferred embodiment of the third aspect, the cosmetic comprises the following components in the following mass percentages: 1-3% of the exfoliating and anti-acne composition, 4-40% of the cosmetic base, and the balance water.

[0040] As a preferred embodiment of the third aspect, the cosmetic matrix includes at least one of a moisturizer, a thickener, a preservative, an emulsifier, a pH adjuster, and a fragrance.

[0041] As a preferred embodiment of the third aspect, the moisturizer includes at least one of glycerin, butylene glycol, and low-molecular sodium hyaluronate; the thickener includes at least one of xanthan gum, carbomer 940, AVC, and high-molecular cellulose; the preservative includes at least one of 1,3-propylene glycol, 1,2-hexanediol, and parahydroxyacetophenone; the emulsifier includes at least one of coconut oil-caprylate / caprate, polydimethylsiloxane, hydrogenated palm kernel oil, cetearyl alcohol, polyglyceryl-6 distearate, and polymethylsilsesquioxane; the pH adjuster includes at least one of arginine and EDTA-2Na.

[0042] Compared with the prior art, the present invention has the following beneficial effects:

[0043] The present invention has found from numerous plant extracts that hydrolyzed prickly pear cactus flower extract, guaiac wood extract, grapefruit seed oil, and rosa centifolia flower water have a synergistic effect with papain. By forming a composition through specific compatibility, it has the ability to gently exfoliate, regulate water-oil balance, protect the skin barrier, and improve acne. The composition provided by the present invention can promote exfoliation and promote the natural replacement of keratin; effectively moisturize and replenish water, reduce excessive oil secretion; reduce inflammatory factors, protect the skin barrier; improve skin acne, and improve skin health as a whole. The present invention provides a mild exfoliation, efficient acne removal composition and its application. Sensitive skin subjects can use cosmetics containing the composition with mild and non-irritating effects, and have the effects of mild exfoliation, regulating water-oil balance, protecting the skin barrier, and improving acne. It is suitable for sensitive skin, has a wide range of applications, and is highly accepted by consumers. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 This is a picture showing the improvement of facial scales before and after using different lotions for 28 days;

[0045] Figure 2 This chart shows the improvement of facial acne on the human body before and after using different lotions for 28 days. DETAILED DESCRIPTION

[0046] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below with reference to specific embodiments. Its purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Unless otherwise specified, the experimental reagents and instruments designed for the implementation and comparative examples of the present invention are all commonly used ordinary reagents and instruments, which can be obtained from commercial channels. In the implementation and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are the same batch of raw materials. Further explanation of the raw materials used in the present invention:

[0047] The preparation method of grapefruit seed oil 1 is as follows:

[0048] (1) Taking grapefruit seeds, crushing them, and drying them in an oven at 45° C. to obtain grapefruit seed powder;

[0049] (2) Weigh a certain amount of grapefruit seed powder, add distilled water at a material-liquid ratio of 1 g:5 ml, stir thoroughly, and place in a 90°C water bath for 1 h;

[0050] (3) After cooling to room temperature, 1% (w / w) alkaline protease was added for enzymatic hydrolysis at 45°C for 2 h.

[0051] (4) After the enzymatic hydrolysis is completed, the emulsion layer is separated by centrifugation at 5000 r / min for 10 min to break the emulsion. The emulsion layer is centrifuged again at 5000 r / min for 10 min for the second centrifugation, and the upper oil phase is taken to obtain the free oil;

[0052] (5) The free oil was placed in a drying oven and dried at 100°C to a constant weight to obtain grapefruit seed oil 1.

[0053] The preparation method of grapefruit oil 2 differs from that of grapefruit oil 1 in the following aspects:

[0054] (a) The material-liquid ratio of water added and mixed in step (1) is 1 g:4 ml; the water bath temperature is 80° C., and the time is 1 hour;

[0055] (b) In step (2), the enzymatic hydrolysis temperature is 50° C. and the enzymatic hydrolysis time is 2 h;

[0056] (c) In step (3), the centrifugation conditions for both times were 3000 r / min and centrifugation for 10 min.

[0057] The preparation method of grapefruit oil 3 differs from that of grapefruit oil 1 in the following ways:

[0058] (a) The material-liquid ratio of water added in step (1) is 1 g:8 ml; the water bath temperature is 90° C., and the time is 2 h;

[0059] (b) In step (2), the enzymatic hydrolysis temperature is 40° C. and the enzymatic hydrolysis time is 3 h;

[0060] (c) In step (3), the centrifugation conditions for both times were 4000 r / min and centrifuged for 15 min.

[0061] The only difference between the preparation method of grapefruit seed oil 4 and the preparation method of grapefruit seed oil 1 is the use of cellulase.

[0062] The preparation method of grapefruit seed oil 5 is as follows:

[0063] Grapefruit seeds were crushed and dried in a 45°C oven to obtain grapefruit seed powder. A certain amount of grapefruit seed powder was weighed and added to 70% ethanol (volume fraction) at a solid-liquid ratio of 1g:5ml. Extraction was carried out under reflux at 80°C for 1 hour. The emulsion layer was separated and demulsified by centrifugation at 5000 rpm for 10 minutes. The free oil was then centrifuged a second time under the same conditions to obtain the free oil. The oil was then placed in a drying oven and dried at 100°C to constant weight to obtain grapefruit seed oil-5.

[0064] Hydrolyzed Opuntia cactus flower extract: The product name is EEO cactus enzyme was purchased from Guangzhou Huayang Biotechnology Co., Ltd.;

[0065] Rose flower extract: The product name is rose flower extract, purchased from Xi'an Xirun Biotechnology Co., Ltd.

[0066] Grape seed oil: The product name is grape seed oil, purchased from Ji'an Huatianbao Chinese Herbal Medicine Biological Products Factory;

[0067] Rosa centifolia flower water: the product name is Rosaliss TM , purchased from Ashland;

[0068] Centella asiatica floral water: The product name is Yanwen Centella asiatica floral water, purchased from Guangzhou Fanshu International Biotechnology Co., Ltd.

[0069] Papain: The product name is papain, purchased from Beijing Solaibao Technology Co., Ltd.;

[0070] Citric acid: The product name is citric acid (≥99.5%), purchased from Biyuntian Biotechnology Co., Ltd.

[0071] Guaiac wood extract: Product number: L2-DZ-SCB3675-X, purchased from Zhenghe (Guangzhou) Biotechnology Co., Ltd.

[0072] Calendula officinalis extract: The product name is calendula officinalis extract, purchased from Guangzhou Meiyi Biotechnology Co., Ltd.

[0073] The preparation method of the composition for mild skin rejuvenating and highly effective acne removal of the present invention comprises the following steps: weighing components in parts by weight, stirring and mixing them uniformly, and ultrasonicating for 10 minutes to obtain the composition for mild skin rejuvenating and highly effective acne removal.

[0074] Examples and Comparative Examples

[0075] The present application provides a composition for gentle skin rejuvenation and efficient acne removal, the components and weight portions of the composition are shown in Table 1.

[0076] Table 1:

[0077]

[0078]

[0079] Effect example: Keratinocyte renewal test

[0080] This effect example tests the effects of the compositions of the examples and comparative examples on promoting cell renewal:

[0081] 1. Experimental materials:

[0082] Phosphate-buffered saline (PBS), Shanghai Solebol; MTT, Yuanye Biotechnology; dimethyl sulfoxide (DMSO), Tianjin Damao; 1× DMEM (high-glucose medium), Gibco; fetal bovine serum (FBS), Gibco; keratinocytes were purchased from the Guangzhou Customs Technology Center; and keratinocytes were purchased from the Kunming Cell Bank of the Chinese Academy of Sciences.

[0083] 2. Experimental steps:

[0084] The cell suspension was inoculated into a 96-well cell culture plate and returned to the incubator for 18-24 hours. The complete culture medium in the well was aspirated, PBS / solvent was added, and the plate was exposed to UVB light (irradiation intensity: 80-90 μW / cm 2 , irradiation dose: 80mJ / cm 2 ), different groups of UVB light treatment are as follows:

[0085] Blank control group: No irradiation was performed. Complete culture medium was added to the blank control group and cultured for 24 h.

[0086] Experimental group: After irradiation for 15 minutes, the PBS solution was aspirated, and complete culture medium was added to the experimental group. 100 μL of 0.5% test sample (compositions of Examples 1-16 and Comparative Examples 1-12) was added to each well and cultured for 24 hours.

[0087] Control group: After 15 min of irradiation, PBS or solvent was added to the control group and cultured for 24 h.

[0088] Model group: After 15 min of irradiation, the PBS solution was aspirated, and complete culture medium was added to the experimental group and cultured for 24 h.

[0089] Remove the culture plate and add 20 μL of thiazolyl blue (MTT) solution to each well (MTT reduces exogenous MTT to water-insoluble, blue-purple crystalline formazan, which deposits in cells; dead cells do not). Incubate in an incubator for 3-4 hours. Remove the liquid from the wells and add 100 μL of DMSO to each well (DMSO dissolves formazan in cells. Its absorbance at 570 nm is measured using an enzyme-linked immunosorbent assay (ELISA) and indirectly reflects the number of viable cells). After incubation on an oscillator for 10-15 minutes, measure the absorbance at 570 nm on a microplate reader and calculate cell viability. All experiments were repeated at least three times according to the principle of parallel design.

[0090] The experimental data were expressed as mean ± SD, and the data were compared and analyzed using T test. Compared with the blank control, # is indicated, #P<0.05, significant; ##P<0.01, extremely significant;

[0091] ###P<0.001, very significant. Compared with the model group, * is indicated by *P<0.05, significant; **P<0.01, significantly significant; ***P<0.001, very significant. The cell viability calculation formula is as follows:

[0092] Keratinocyte viability (%) = (OD value of sample well - OD value adjusted to zero) / (OD value of blank control well - OD value adjusted to zero) × 100%. A greater keratinocyte viability (%) indicates a stronger effect of the combination in promoting keratin renewal.

[0093] Table 2:

[0094] Group Keratinocyte viability (%) Group Keratinocyte viability (%) Blank control group 100.00 Model Group <![CDATA[58.70 ## ]]> Example 1 71.45* Comparative Example 1 61.83 Example 2 75.23* Comparative Example 2 62.05 Example 3 78.78** Comparative Example 3 55.20 Example 4 79.23** Comparative Example 4 56.31 Example 5 81.01** Comparative Example 5 55.53 Example 6 82.23** Comparative Example 6 55.90 Example 7 86.45** Comparative Example 7 56.20 Example 8 83.67** Comparative Example 8 59.53 Example 9 83.10** Comparative Example 9 58.90 Example 10 86.52** Comparative Example 10 58.75 Example 11 82.34** Comparative Example 11 59.23 Example 12 88.78** Comparative Example 12 58.01 Example 13 82.56** Example 14 81.45** Example 15 81.12** Example 16 80.21**

[0095] As can be seen from Table 1, the mild skin rejuvenating and highly effective acne-removing composition provided by the present invention can inhibit the death of keratinocytes after UV irradiation, promote the formation of new keratinocytes, and effectively enhance the ability of keratinocyte renewal, which indicates that the composition of the present invention has the effect of promoting keratin renewal.

[0096] As can be seen from Examples 1-5, the mass ratio of the components in the composition of the present invention can affect the ability of the composition to promote corneocyte renewal. When the mass parts of the components are limited to the mass ratio of component A, component B and component C (1.1-6): (1.01-11): (0.01-0.1) range, the resulting composition has a good effect of renewing keratin. When the mass ratio of component A, component B and component C is further selected to be within the range of (1.5-4): (2.05-8.8): (0.04-0.08), the resulting composition has a better effect of promoting keratin metabolism. When the mass ratio of component A, component B and component C is 2: 6.7: 0.06 (Example 5), the effect of promoting keratin metabolism is best.

[0097] The present invention studies the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract in component A through Examples 6-8. It can be seen from Examples 6-8 that the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract affects the keratinocyte viability of the composition, thereby affecting the composition's ability to promote keratin renewal. Therefore, when the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract is limited to the range of (0.1-3): (1-3), the ability of the composition to promote keratin renewal can be improved. When the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract is further selected to be within the range of (0.3-2): (1.2-2), the effect of the resulting composition is even better. When the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract is 0.5:1.5 (Example 7), its effect of promoting keratin renewal reaches the best.

[0098] The present invention studies the mass ratio of grapefruit seed oil to Rosa centifolia flower water through Examples 9-11. It can be seen from Examples 9-11 that the mass ratio of grapefruit seed oil to Rosa centifolia flower water affects the keratinocyte viability of the composition, thereby affecting the composition's ability to promote keratin renewal. Therefore, when the mass ratio of grapefruit seed oil to Rosa centifolia flower water is limited to the range of (0.01-1): (1-10), the keratinizing ability of the composition can be improved. When the mass ratio of grapefruit seed oil to Rosa centifolia flower water is further selected to be within the range of (0.05-0.8): (2-8), the effect of the resulting composition is even better. When the mass ratio of grapefruit seed oil to Rosa centifolia flower water is 0.4:6.3 (Example 10), its keratinizing effect reaches the best.

[0099] In summary, the composition with the best distribution ratio of each group was obtained: hydrolyzed Opuntia pyrifolia flower extract: Guaiac wood extract: grapefruit seed oil: Rosa centifolia flower water: papain = 0.5:1.5:0.4:6.3:0.06 (Example 12).

[0100] The present invention investigated the effects of grapefruit seed oil obtained by different extraction methods on the exfoliation ability of compositions using Examples 12-16. These Examples demonstrate that the extraction method of grapefruit seed oil has a certain impact on the exfoliation effect. The composition obtained using grapefruit seed oil 1 exhibited the best exfoliation effect (Example 12). The exfoliation effects of compositions obtained using other extraction methods in Examples 13-16 were inferior to those of Example 12.

[0101] It can be seen from Comparative Examples 1-2 that the exfoliating effect of Comparative Examples 1-2 is poor. This is because the mass ratios of the components in the compositions of Comparative Examples 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0102] It can be seen from Comparative Examples 3-7 that the components in the composition provided by the present invention are indispensable. When any one of them is missing, the keratinocyte activity of the obtained composition is significantly reduced.

[0103] It can be seen from Comparative Examples 8-12 that when other similar ingredients are used to replace the components in the composition of the present invention, the corresponding effects of the present invention cannot be achieved.

[0104] Application Examples

[0105] The application examples and comparative application examples of the present invention provide an emulsion, the components (mass percentage) of the emulsion are shown in Table 3; wherein, the mild skin-rejuvenating and highly effective acne-removing compositions used in Application Examples 1-16 are respectively the compositions prepared in Examples 1-16, and the mild skin-rejuvenating and highly effective acne-removing compositions used in Application Examples 17 and 18 are respectively the compositions prepared in Example 1; the mild skin-rejuvenating and highly effective acne-removing compositions used in Comparative Application Examples 1-12 are respectively the compositions prepared in Comparative Examples 1-12; a blank application example is also provided, the only difference between the blank application example and Application Example 1 is that the mild skin-rejuvenating and highly effective acne-removing compositions are not added; see Table 3 for details:

[0106] Table 3:

[0107]

[0108]

[0109] The preparation method of the emulsion in Application Example 1 is:

[0110] (1) Mix phase A with water, heat to 80°C, and homogenize at 1200 rpm for 5 minutes. After homogenization, keep warm and set aside to obtain prefabricated phase A;

[0111] (2) Mix phase B, heat to 80°C, and homogenize at 1200 rpm for 5 min. After homogenization, keep warm and set aside to obtain prefabricated phase B;

[0112] (3) Mix phase C and heat to 60°C to melt to obtain prefabricated phase C;

[0113] (4) Heat the preformed phase A to 80°C, add the preformed phase B at a speed of 300 rpm, stir and mix, then cool to 60°C, add the preformed phase C at a speed of 300 rpm, stir and mix, then cool to below 45°C, add the anti-inflammatory and anti-acne composition and essence, and continue stirring for 5 minutes. Finally, add arginine to adjust the pH to 5.0-6.5, then stop stirring, discharge, and obtain an emulsion.

[0114] Test example: Detect skin scaling index, TEWL, moisturizing degree, and acne count of each lotion in the application example

[0115] This test examined the improvement effects of Application Examples 1-18, Comparative Application Examples 1-12, and Blank Application Examples on human skin TEWL, roughness, and acne.

[0116] The specific test methods are as follows:

[0117] According to the "Technical Specifications for Safety of Cosmetics" (2015), 155 volunteers aged 18-50 with sensitive skin (lactic acid stinging score > 3) were selected and randomly divided into 31 groups, with 5 people in each group. The volunteers had obvious acne. Under normal circumstances, the volunteers continued to use the product on their entire face for 28 days; every morning and evening after cleansing and moisturizing, the volunteers took an appropriate amount of lotion and applied it to the face, massaging it for absorption. The acne area could be overlapped and applied to the entire face once in the morning and evening, and follow-up visits were conducted every day. On the day of the volunteer's visit, the test area washed its face with clean water without applying any product. It was then placed in an air-conditioned room with a temperature of 21±1°C and a humidity of 50±10% for 20 minutes.

[0118] The improvement of facial acne indicators was quantified and photographed using instruments, including acne counting (categorized counting: comedones and papules, using VISIA and doctor assessment respectively), Tewameter TM Hex test for transepidermal water loss (TEWL), Corneometer CM 825 test for moisturizing, and VC 20 Plus test for scaling index.

[0119] Experimental data are expressed as mean ± standard deviation (mean ± SD), and data comparison and analysis were performed using the T test. Compared with the blank control, * is indicated by *P < 0.05, significant; **P < 0.01, significantly significant; ***P < 0.001, very significantly significant. Improvement rate before and after use (%) = (X mean before use - X mean after use) / X mean before use × 100%;

[0120] Among them: the greater the improvement rate of the scale index (%), the stronger the exfoliation ability of the lotion; the greater the improvement rate of papules and acne (%), the stronger the acne removal ability of the lotion; the greater the improvement rate of the TEWL value, the stronger the skin barrier damage repair ability of the lotion; the greater the improvement rate of the moisturizing (%), the stronger the moisturizing and hydrating ability of the lotion. The calculated results are shown in Table 4:

[0121] Table 4:

[0122]

[0123]

[0124] As can be seen from Table 4, the emulsion provided by the present invention exhibits excellent mild exfoliation, skin barrier repair, acne treatment, and moisturizing effects. Specifically, on day 28, the achieved scaling index improvement rate was greater than 12%, the TEWL value improvement rate was greater than 8%, the papule improvement rate was greater than 17%, the acne improvement rate was greater than 12%, and the moisturizing rate was greater than 25%. As can be seen from Application Examples 1-12, the weight percentage of the components of the exfoliating and anti-acne composition added to the emulsion affects the overall performance of the product.

[0125] As can be seen from Application Examples 1-5, the mass ratio of each component in the composition of the emulsion affects the scale index, TEWL value, papule and acne improvement, and moisturizing ability of the composition. When the mass ratio of the components is limited to the mass ratio of component A, component B, and component C within the range of (1.1-6): (1.01-11): (0.01-0.1), the resulting composition can reduce the scale index of the skin, improve the TEWL value, the papule and acne improvement rate, and the moisturizing ability of the emulsion, thereby enhancing the overall comprehensive effect of the emulsion. When the mass ratio of component A, component B, and component C is further selected within the range of (1.5-4): (2.05-8.8): (0.04-0.08), the effect of the resulting composition is even better. When the mass ratio of component A, component B, and component C is 2:6.7:0.06 (Application Example 5), the overall effect is optimal.

[0126] The present invention studies the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract in component A through application examples 6-8. It can be seen from application examples 6-8 that the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract affects the TEWL value and moisturizing improvement rate of the emulsion, thereby affecting the barrier repair and moisturizing ability of the emulsion. Therefore, when the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract is limited to the range of (0.1-3):(1-3), the barrier repair and moisturizing ability of the emulsion can be improved. When the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract is further selected to be within the range of (0.3-2):(1.2-2), the effect of the obtained emulsion is even better. When the mass ratio of the hydrolyzed prickly pear cactus flower extract to the guaiac wood extract is 0.5:1.5 (application example 7), its barrier repair and moisturizing ability effects are optimal.

[0127] The present invention studies the mass ratio of grapefruit seed oil to Rosa centifolia floral water through application examples 9-11. It can be seen from application examples 9-11 that the mass ratio of grapefruit seed oil to Rosa centifolia floral water affects the improvement rate of papules and acne of the emulsion, thereby affecting the acne-removing ability of the emulsion. Therefore, when the mass ratio of grapefruit seed oil to Rosa centifolia floral water is limited to the range of (0.01-1):(1-10), the acne-removing ability of the emulsion can be improved. When the mass ratio of grapefruit seed oil to Rosa centifolia floral water is further selected to be within the range of (0.05-0.8):(2-8), the effect of the obtained emulsion is even better. When the mass ratio of grapefruit seed oil to Rosa centifolia floral water is 0.4:6.3 (application example 10), the acne-removing effect is optimal.

[0128] In summary, the composition with the best distribution ratio of each group was obtained: hydrolyzed prickly pear cactus flower extract: guaiac wood extract: grapefruit seed oil: Rosa centifolia flower water: papain = 0.5:1.5:0.4:6.3:0.06 (Application Example 12).

[0129] The present invention investigated the effects of grapefruit seed oil obtained by different extraction methods on the acne-removing ability of a composition through Application Examples 12-16. Application Examples 12-16 demonstrate that the extraction method of grapefruit seed oil has a certain impact on the acne-removing effect, thereby affecting the acne-removing efficacy of the composition. The composition obtained using grapefruit seed oil 1 exhibited the best anti-inflammatory effect (Application Example 12). The acne-removing effects of Application Examples 13-16 using other extraction methods were inferior to those of Application Example 12.

[0130] It can be seen from Application Example 1 and Comparative Application Examples 1-2 that the moisturizing, acne-removing, exfoliating, and skin barrier repair effects of Comparative Application Examples 1-2 are poor. This is because the mass ratios of the components in the compositions of Comparative Examples 1-2 are not within the scope of the present invention, which affects the synergistic effect between the components and reduces the efficacy of the composition.

[0131] It can be seen from Application Example 1 and Comparative Application Examples 3-7 that the components in the composition of the emulsion provided by the present invention are indispensable. When any one of them is missing, the overall performance of the obtained emulsion is significantly reduced.

[0132] It can be seen from Application Example 1 and Comparative Application Examples 8-12 that when other similar ingredients are used to replace the components in the composition of the present invention, the corresponding effects of the present invention cannot be achieved.

[0133] Among them, the photographs of volunteers before and after use of the emulsions in the blank application example, application example 1 and comparative application example 8 are as follows: Figure 1 、 2 As shown, from Figure 1 It can be seen intuitively that the emulsion provided by the present invention can effectively improve human facial squama (the less red part in the figure, the better the squama improvement effect), achieving the purpose of exfoliation; Figure 2 It can be seen intuitively that the emulsion provided by the present invention can effectively improve human facial acne and achieve the purpose of removing acne.

[0134] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for gentle skin rejuvenation and efficient acne removal, characterized in that: The composition comprises component A, component B and component C; The component A comprises hydrolyzed opuntia cactus flower extract and guaiac extract; The component B includes grapefruit seed oil and Rosa centifolia flower water; The component C is papain; The mass ratio of component A, component B and component C in the composition is component A: component B: component C = (1.1-6): (1.01-11): (0.01-0.1); In the component A, the mass ratio of the hydrolyzed Opuntia cactus flower extract and the Guaiac wood extract is hydrolyzed Opuntia cactus flower extract:Guaiac wood extract=(0.1-3):(1-3); In the component B, the mass ratio of grapefruit seed oil to Rosa centifolia flower water is grapefruit seed oil: Rosa centifolia flower water = (0.01-1): (1-10).

2. The composition according to claim 1, wherein The mass ratio of component A, component B and component C in the composition is component A: component B: component C = (1.5-4): (2.05-8.8): (0.04-0.08).

3. The composition according to claim 1, wherein In the component A, the mass ratio of the hydrolyzed opuntia flower extract and the guaiac extract is hydrolyzed opuntia flower extract:guaiac extract=(0.3-2):(1.2-2).

4. The composition according to claim 1, wherein In the component B, the mass ratio of grapefruit seed oil to Rosa centifolia flower water is grapefruit seed oil: Rosa centifolia flower water = (0.05-0.8): (2-8).

5. The composition according to any one of claims 1 to 4, wherein The preparation method of the grapefruit seed oil comprises the following steps: (1) Take grapefruit seeds, dry them, crush them, add water and mix them in a water bath; (2) adding protease to perform enzymolysis to obtain an enzymolysis product; (3) centrifuging the enzymatic hydrolysate to break the emulsion, separating the emulsion layer, centrifuging the emulsion layer, and taking the upper oil phase to obtain free oil; (4) drying the free oil to a constant weight to obtain the grapefruit seed oil.

6. The composition according to claim 5, wherein Meet at least one of the following: (a) The material-liquid ratio of water added and mixed in step (1) is 1g: (4-8)ml; the water bath temperature is 80-90°C, and the time is 1-3h; (b) The protease in step (2) is alkaline protease, the enzymatic hydrolysis temperature is 40-45°C, and the enzymatic hydrolysis time is 2-3h; (c) The centrifugation conditions in step (3) are both 3000-5000 r / min and 10-20 min.

7. Use of the composition according to any one of claims 1 to 6 in the preparation of cosmetics.

8. The use according to claim 7, characterized in that The cosmetics include one or more of lotion, emulsion, cream, mask, essence, and spray.

9. A cosmetic, characterized in that: The cosmetic comprises the composition according to any one of claims 1 to 6.

10. The cosmetic according to claim 9, wherein The cosmetic comprises the following components in percentage by mass: 1-5% of the composition according to any one of claims 1 to 6, 4-40% of a cosmetic base, and the balance being water.

11. The cosmetic according to claim 10, wherein The cosmetic matrix includes at least one of a moisturizer, a thickener, a preservative, an emulsifier, a pH regulator, and a fragrance.

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