A kind of notoginseng and salvia miltiorrhiza composite material and preparation method thereof

Abstract

The invention discloses a Sanqi Danshen composition and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine. The invention discloses a Sanqi Danshen composition, and the components include Sanqi, Danshen, honeysuckle, Codonopsis, Lycium barbarum and Schisandra chinensis. According to the mass fraction, the components include: 15-25 parts of Sanqi, 15-20 parts of Danshen, 8-15 parts of honeysuckle, 7-12 parts of Codonopsis pilosula, 5-10 parts of Lycium barbarum and 4-6 parts of Schisandra chinensis. The Sanqi Danshen composition provided by the present invention can improve the immune function of the human body and can protect the human body from the invasion of diseases at multiple levels. The present invention provides a reliable method for enhancing immunity, which is expected to achieve better results in clinical applications.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a notoginseng and salvia miltiorrhiza composition and a preparation method thereof. Background Art

[0003] The effect of existing Chinese medicine compositions in enhancing immunity still needs to be improved. Although many Chinese medicine compositions contain some medicinal materials with immunomodulatory effects, their formulas are often relatively simple and lack systematic research and verification. This results in the instability of the effects of these compositions in practical applications, making it difficult to meet people's needs for enhancing immunity. In addition, some Chinese medicine compositions may have side effects or adverse reactions during use, which also limits their scope of application. Therefore, it is an urgent problem to develop a new Chinese medicine composition that fully utilizes the synergistic effects of various medicinal materials through reasonable Chinese medicine compatibility to achieve a more effective effect of enhancing immunity. Summary of the invention

[0004] The purpose of the present invention is to provide a notoginseng and salvia miltiorrhiza composition and a preparation method thereof to solve the problems existing in the above-mentioned prior art. This new composition can not only improve the immune function of the human body, but also has multiple effects such as anti-oxidation and anti-inflammatory, and can protect the human body from diseases at multiple levels.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] Technical solution 1: A Panax notoginseng and Salvia miltiorrhiza composite, comprising Panax notoginseng, Salvia miltiorrhiza, honeysuckle, Codonopsis pilosula, Lycium barbarum and Schisandra chinensis.

[0007] Effects of Panax notoginseng: promoting blood circulation and removing blood stasis, stopping bleeding and promoting tissue regeneration, reducing swelling and relieving pain. Main ingredients: Panax notoginseng saponins, flavonoid compounds, etc.

[0008] Danshen effects: promoting blood circulation and removing blood stasis, cooling blood and reducing swelling, clearing the heart and relieving restlessness. Main ingredients: tanshinone, salvianolic acid, etc.

[0009] Honeysuckle has the following effects: clearing away heat and detoxifying, dispersing wind-heat, and anti-inflammatory and antibacterial. Main ingredients: chlorogenic acid, flavonoid compounds, etc.

[0010] Codonopsis pilosula effects: tonifying qi, strengthening spleen and lungs, nourishing blood and promoting body fluid. Main ingredients: polysaccharides, saponins, alkaloids, etc.

[0011] Effects of wolfberry: nourishes liver and kidney, improves eyesight, moistens lungs. Main ingredients: polysaccharides, carotene, vitamin C, etc.

[0012] Schisandrae Chinensis Effects: astringent, invigorating Qi and promoting the production of body fluids, nourishing the kidney and calming the mind. Main ingredients: Schisandrae Chinensis Alcohol A, Schisandrae Chinensis Ferment, etc.

[0013] Furthermore, the components include, by weight: 15-25 parts of Panax notoginseng, 15-20 parts of Salvia miltiorrhiza, 8-15 parts of Honeysuckle, 7-12 parts of Codonopsis pilosula, 5-10 parts of Lycium barbarum and 4-6 parts of Schisandra chinensis.

[0014] Furthermore, the components include, by weight: 25 parts of Panax notoginseng, 20 parts of Salvia miltiorrhiza, 10 parts of Honeysuckle, 7-12 parts of Codonopsis pilosula, 5-10 parts of Lycium barbarum and 4-6 parts of Schisandra chinensis.

[0015] Technical solution 2: Use of the Panax notoginseng and Salvia miltiorrhiza composition in the preparation of drugs for enhancing immunity.

[0016] Technical solution three: A medicine with the function of enhancing immunity, wherein the effective ingredients of the medicine include the Panax notoginseng and Salvia miltiorrhiza composition.

[0017] Furthermore, the drug also includes pharmaceutically acceptable excipients.

[0018] Technical Solution 4: An extract obtained by extracting the Panax notoginseng and Salvia miltiorrhiza composition, the preparation method of the extract comprising the following steps: the components in the Panax notoginseng and Salvia miltiorrhiza composition are crushed separately and then mixed, and the extract is obtained by refluxing with an alcohol solution.

[0019] Furthermore, the volume concentration of alcohol in the alcohol solution is 85%.

[0020] Furthermore, the conditions of the reflux extraction include: temperature of 75° C. and time of 4 hours.

[0021] Specifically, the preparation method includes: firstly, refluxing the crushed and mixed components in the Panax notoginseng and Salvia miltiorrhiza composition with an alcohol solution, and then reducing the pressure and concentrating until there is no alcohol taste to obtain a suspended aqueous solution; extracting with petroleum ether and n-butanol in sequence, reducing the pressure, concentrating and drying to obtain the extract of the Panax notoginseng and Salvia miltiorrhiza composition.

[0022] In the present invention, in order to further improve the effect of the extract in enhancing immunity, the preparation method of the extract comprises:

[0023] The components in the Panax notoginseng and Salvia miltiorrhiza composite are crushed and mixed to obtain a mixture; the mixture is refluxed and extracted with an alcohol solution, the operation is repeated twice, the filtrate is combined, the pressure is reduced, and the concentration is performed until there is no alcohol taste to obtain a suspension; an equal volume of petroleum ether is added for extraction, the operation is repeated twice, and the upper liquid is combined to obtain a petroleum ether layer; the lower liquid layer is taken, and an equal volume of n-butanol is added, the operation is repeated twice, the upper liquid is combined to obtain an n-butanol layer, and the lower layer is the extract water layer of the mixture of the components in the Panax notoginseng and Salvia miltiorrhiza composite; the organic solvent and water in each component are removed to obtain an extract.

[0024] The crushing method has no special requirements, and conventional methods in the art can be used. The crushing also includes screening, and the sieve mesh is 30 mesh.

[0025] There is no special requirement for the extraction, and conventional methods in the art may be used.

[0026] In the above preferred embodiment of the present invention, in order to better remove the solvent, the drying method is reduced pressure drying.

[0027] Technical Solution 5: Use of the extract in the preparation of drugs having the function of enhancing immunity.

[0028] Furthermore, the dosage form of the drug includes decoction, pill, granule, injection, paste, tablet, capsule, syrup, drops and other forms. The above various dosage forms of drugs can be prepared according to conventional methods in the pharmaceutical field. The administration method of the drug includes common administration methods such as oral, sublingual, intravenous, intramuscular, and nasogastric feeding.

[0029] The present invention discloses the following technical effects:

[0030] The invention discloses a composition comprising a plurality of Chinese medicinal materials including Panax notoginseng, Salvia miltiorrhiza, honeysuckle, Codonopsis pilosula, Lycium barbarum and Schisandra chinensis, aiming to provide a safe, effective and comprehensive Chinese medicinal composition for enhancing immunity through the synergistic effect of these medicinal materials. Panax notoginseng, Salvia miltiorrhiza, honeysuckle, Codonopsis pilosula, Lycium barbarum and Schisandra chinensis are all commonly used Chinese medicinal materials, each having a unique pharmacological effect. Panax notoginseng and Salvia miltiorrhiza both have the effect of promoting blood circulation and removing blood stasis, can synergistically enhance blood circulation, improve microcirculation, and are suitable for the prevention and treatment of cardiovascular diseases. Honeysuckle has the effects of clearing away heat and detoxifying, anti-inflammatory and antibacterial, and can cooperate with other medicinal materials to enhance the overall anti-inflammatory and immunomodulatory effects. Codonopsis pilosula can replenish the middle qi, strengthen the spleen and benefit the lungs, and help improve the overall immunity and resistance of the body. Lycium barbarum nourishes the liver and kidneys, improves eyesight and moisturizes the lungs, can supplement the body's nutrition and enhance physical fitness. Schisandra chinensis has the effects of astringent and astringent, invigorating qi and promoting body fluid, and can enhance the body's tolerance and resistance. The composition provided by the present invention can improve the immune function of the human body and protect the human body from diseases at multiple levels. The present invention provides a reliable method for enhancing immunity, which is expected to achieve better results in clinical applications. DETAILED DESCRIPTION

[0031] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0032] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0033] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0034] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0035] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0036] The preparation method of the Panax notoginseng and Salvia miltiorrhiza composition of the present invention is not limited to the reflux extraction of the Panax notoginseng and Salvia miltiorrhiza composition, but also includes the following exemplary methods: after mixing the components, adding water to decoct, filtering the decoction to obtain a solution, and concentrating the obtained solution to obtain an extract; mixing the components and sieving them, crushing and fully mixing them with the auxiliary agents commonly used in preparing tablets, passing them through a 50-mesh sieve, and directly pressing the dry powder into tablets by a tablet press; sieving the mixture of the components, crushing and fully mixing them with the auxiliary agents commonly used in preparing tablets, and then filling the capsules, polishing, and packaging them after granulation, drying, granulation, etc. to obtain the finished product.

[0037] Example 1

[0038] Preparation of extract of Panax notoginseng and salvia miltiorrhiza composite

[0039] Take each component of the dried Panax notoginseng and Salvia miltiorrhiza composite (25 parts of Panax notoginseng, 20 parts of Salvia miltiorrhiza, 10 parts of honeysuckle, 8 parts of Codonopsis pilosula, 6 parts of Lycium barbarum and 4 parts of Schisandra chinensis), grind them, pass through a 30-mesh sieve and mix them, add 20 times the volume of 85% ethanol solution, reflux and extract at 75°C, reflux for 4 hours each time, repeat this operation twice to obtain an extract, combine the extracts, and concentrate under reduced pressure until the ethanol is completely volatilized (no alcohol taste) to obtain a suspension; then extract with an equal volume of petroleum ether, repeat this operation twice, combine the upper petroleum ether ether extract; the extract is concentrated under reduced pressure and dried to obtain a powder, which is the petroleum ether component of the extract, and is stored in a refrigerator at 4°C for later use; the lower aqueous suspension solution is then extracted with an equal volume of water-saturated n-butanol, extracted twice in total, the upper water-saturated n-butanol extract is combined, the extract is concentrated under reduced pressure and dried to obtain a powder, which is the n-butanol component of the extract, and is stored in a refrigerator at 4°C for later use; finally, the lower aqueous suspension solution is collected, concentrated, and dried under reduced pressure to obtain a powder, which is the extract of the Panax notoginseng and Salvia miltiorrhiza composite.

[0040] Example 2

[0041] Preparation of extract of Panax notoginseng and salvia miltiorrhiza composite

[0042] Take each component of the dried Panax notoginseng and Salvia miltiorrhiza composite (15 parts of Panax notoginseng, 15 parts of Salvia miltiorrhiza, 8 parts of honeysuckle, 10 parts of Codonopsis pilosula, 5 parts of Lycium barbarum and 5 parts of Schisandra chinensis), grind them, pass through a 30-mesh sieve and mix them, add 20 times the volume of 85% ethanol solution, reflux and extract at 75°C, reflux for 4 hours each time, repeat this operation twice to obtain an extract, combine the extracts, and concentrate under reduced pressure until the ethanol is completely volatilized (no alcohol taste) to obtain a suspension; then extract with an equal volume of petroleum ether, repeat this operation twice, combine the upper petroleum ether ether extract; the extract is concentrated under reduced pressure and dried to obtain a powder, which is the petroleum ether component of the extract, and is stored in a refrigerator at 4°C for later use; the lower aqueous suspension solution is then extracted with an equal volume of water-saturated n-butanol, extracted twice in total, the upper water-saturated n-butanol extract is combined, the extract is concentrated under reduced pressure and dried to obtain a powder, which is the n-butanol component of the extract, and is stored in a refrigerator at 4°C for later use; finally, the lower aqueous suspension solution is collected, concentrated, and dried under reduced pressure to obtain a powder, which is the extract of the Panax notoginseng and Salvia miltiorrhiza composite.

[0043] Example 3

[0044] Preparation of extract of Panax notoginseng and salvia miltiorrhiza composite

[0045] Take each component of the dried Sanqi Danshen composite (25 parts of Sanqi, 20 parts of Danshen, 15 parts of honeysuckle, 12 parts of Codonopsis, 10 parts of Lycium barbarum and 6 parts of Schisandra chinensis), grind them, pass through a 30-mesh sieve and mix them, add 20 times the volume of 85% ethanol solution, reflux extraction at 75°C, reflux for 4 hours each time, repeat this operation twice to obtain an extract, combine the extracts, and concentrate under reduced pressure until the ethanol is completely volatilized (no alcohol taste) to obtain a suspension; then extract with an equal volume of petroleum ether, repeat this operation twice, combine the upper layer of petroleum ether, and extract. The extract of oil ether; the extract is concentrated under reduced pressure and dried to obtain a powder, which is the petroleum ether component of the extract, and is placed in a refrigerator at 4°C for standby use; the lower aqueous suspension solution is then extracted with an equal volume of water-saturated n-butanol, extracted twice in total, the upper water-saturated n-butanol extract is combined, the extract is concentrated under reduced pressure and dried to obtain a powder, which is the n-butanol component of the extract, and is placed in a refrigerator at 4°C for standby use; finally, the lower aqueous suspension solution is collected, concentrated, and dried under reduced pressure to obtain a powder, which is the extract of the Panax notoginseng and Salvia miltiorrhiza composite.

[0046] Comparative Example 1

[0047] Compared with Example 1, the components of the Panax notoginseng and Salvia miltiorrhiza composition in this comparative example are: 30 parts of Panax notoginseng, 25 parts of Salvia miltiorrhiza, 20 parts of Honeysuckle, 15 parts of Codonopsis pilosula, 3 parts of Lycium barbarum and 2 parts of Schisandra chinensis. The remaining steps are the same as in Example 1.

[0048] Comparative Example 2

[0049] Preparation of extract of Panax notoginseng and salvia miltiorrhiza composite

[0050] Take each component of the dried Panax notoginseng and Salvia miltiorrhiza composite (25 parts of Panax notoginseng, 20 parts of Salvia miltiorrhiza, 10 parts of honeysuckle, 8 parts of Codonopsis pilosula, 6 parts of Lycium barbarum and 4 parts of Schisandra chinensis), grind them and pass them through a 30-mesh sieve, mix them, add 20 times the volume of an ethanol solution with a volume concentration of 85%, reflux extraction at 75°C, reflux for 4 hours each time, obtain an extract, and concentrate under reduced pressure until all ethanol is volatilized (without alcohol taste) to obtain a suspension; then extract with an equal volume of petroleum ether, concentrate the upper layer of petroleum ether extract under reduced pressure and dry, and the obtained powder is the petroleum ether component of the extract, and store it in a refrigerator at 4°C for standby use; then extract the lower layer of aqueous suspension solution with an equal volume of water-saturated n-butanol, concentrate the upper layer of water-saturated n-butanol extract under reduced pressure and dry, and the obtained powder is the n-butanol component of the extract, and store it in a refrigerator at 4°C for standby use; finally collect the lower layer of aqueous suspension solution, concentrate, and dry it under reduced pressure, and the obtained powder is the extract of the Panax notoginseng and Salvia miltiorrhiza composite.

[0051] Test Example 1

[0052] (1) Safety evaluation of extracts of Panax notoginseng and Salvia miltiorrhiza composite

[0053] The extract powder prepared in Example 1 was subjected to an acute oral experiment on mice. Method: 10 mice were gavaged with 1 mL of the maximum dose (extract powder mixed with purified water at 1 g / mL) at one time, and 1.2 mL of the extract was given 24 hours later, and no mouse death was observed;

[0054] Skin irritation experiment: The mixture of the extract of the present invention and pure water was applied to the back skin of rabbits, and no biting, scratching or redness and swelling were observed in the rabbits, indicating that the mixture of the extract and pure water prepared by the present invention does not cause irritation to the skin.

[0055] (2) Experiment on spleen deficiency model mice

[0056] Male Kunming mice weighing about 20g were selected and randomly divided into groups of 10 mice each. There was no significant difference in the initial weight of each group of mice. These included a blank group, a model group, and research groups 1-5 (respectively corresponding to the extracts of the Sanqi Danshen composition prepared in Examples 1-3 and Comparative Examples 1-2 for research experiments). The blank group was gavaged with normal saline, and the remaining groups of mice were gavaged with senna leaf decoction (10g / kg) and the hunger and satiety method for continuous modeling for 15d. The senna leaf decoction: the senna leaf medicinal material was added to 12 times the amount of water, boiled for 15min, filtered, and the filtrate was recovered and concentrated to a concentration of 1g / mL. Drug administration: Starting from the 8th day, research groups 1-5 were gavaged with extracts of the Sanqi Danshen composition, with a daily dosage of 10mg / kg, and administered for 7 consecutive days. Record the body weight before and after the test, calculate the body weight growth rate, and measure the thymus index and spleen index after the test.

[0057] Table 1 Effects on body weight and immune organ indexes of mice with spleen deficiency and low immune function

[0058]

[0059]

[0060] Compared with the blank group, the body weight and immune organ index of the mice in the model group were significantly reduced, indicating that spleen deficiency had a significant effect on the body weight and immune organ index of the mice, confirming the successful establishment of the model. Compared with the model group, the body weight, thymus index and spleen index of the mice in the study groups 1-5 increased, among which the increase in Example 1-3 was more obvious, indicating that the extracts prepared in Example 1-3 significantly improved spleen deficiency and immune organ index.

[0061] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A Panax notoginseng and Salvia miltiorrhiza composition, characterized in that: The ingredients include Panax notoginseng, Salvia miltiorrhiza, Honeysuckle, Codonopsis pilosula, Lycium barbarum and Schisandra chinensis.

2. The notoginseng and salvia miltiorrhiza composition according to claim 1, characterized in that Calculated by weight, the components include: 15-25 parts of Panax notoginseng, 15-20 parts of Salvia miltiorrhiza, 8-15 parts of honeysuckle, 7-12 parts of Codonopsis pilosula, 5-10 parts of Lycium barbarum and 4-6 parts of Schisandra chinensis.

3. The notoginseng and salvia miltiorrhiza composition according to claim 2, characterized in that Calculated by weight, the components include: 25 parts of Panax notoginseng, 20 parts of Salvia miltiorrhiza, 10 parts of Honeysuckle, 8 parts of Codonopsis pilosula, 6 parts of Lycium barbarum and 4 parts of Schisandra chinensis.

4. Use of the Panax notoginseng and Salvia miltiorrhiza composition according to any one of claims 1 to 3 in the preparation of drugs for enhancing immunity.

5. A drug for enhancing immunity, characterized in that: The active ingredients of the medicine include the Panax notoginseng and Salvia miltiorrhiza composition according to any one of claims 1-3.

6. The drug according to claim 5, characterized in that The drug also includes pharmaceutically acceptable excipients.

7. An extract obtained by extracting the Panax notoginseng and Salvia miltiorrhiza composition according to any one of claims 1 to 3, characterized in that: The preparation method of the extract comprises the following steps: the components in the Panax notoginseng and Salvia miltiorrhiza composite are crushed separately and then mixed, and the extract is obtained by refluxing with an alcohol solution.

8. The extract according to claim 7, characterized in that The volume concentration of alcohol in the alcohol solution is 85%.

9. The extract according to claim 7, characterized in that The reflux extraction conditions include: temperature of 75° C. and time of 4 h.

10. Use of the extract according to claim 7 in preparing a drug for enhancing immunity.

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