An auxiliary lipid-lowering composition, its preparation method and application

Abstract

The present invention relates to the technical field of microbial fermentation, and particularly relates to an auxiliary lipid-lowering composition, a preparation method thereof, and an application thereof. A preparation method of an auxiliary lipid-lowering composition includes Step 1: First, mix apples, white hyacinth beans, red grapes, euryale ferox seeds, and lilies, then add water to form a slurry, and sterilize to obtain a fermentation substrate; Step 2: Inoculate Trichoderma reesei and ferment to obtain an initial fermented product; Step 3: Inoculate Candida krusei and ferment to obtain a second fermented product; Step 4: Take the supernatant of the second fermented product and inoculate Bifidobacterium breve, and ferment to obtain a third fermented product; Step 5: Take the third fermented product, filter and sterilize it to obtain an auxiliary lipid-lowering fermented product; Step 6: Mix the auxiliary lipid-lowering fermented product, freeze-dried natto powder, and ginkgo leaf extract evenly to obtain an auxiliary lipid-lowering composition. The auxiliary lipid-lowering composition of the present application has both safety and high bioavailability.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial fermentation, and in particular to an auxiliary lipid-lowering composition, a preparation method and an application thereof. Background Art

[0002] Dyslipidemia (such as elevated total cholesterol and low-density lipoprotein cholesterol) is the core inducement of cardiovascular and cerebrovascular diseases such as atherosclerosis, coronary heart disease, and stroke, and shows a trend of getting younger. Long-term hyperlipidemia can lead to vascular endothelial injury, lipid deposition and plaque formation, further triggering fatal events such as myocardial infarction and cerebral infarction. In addition, hyperlipidemia is also closely related to metabolic syndromes such as obesity, type 2 diabetes, and non-alcoholic fatty liver. Therefore, regulating blood lipid levels has become a key intervention means for preventing chronic diseases.

[0003] Currently, the mainstream lipid-lowering means include chemical drugs (such as statins and fibrates) and natural functional products (such as red yeast rice extract and phytosterols). However, chemical drugs have relatively large side effects. Long-term use of chemical drugs to lower blood lipids may lead to abnormal liver function, muscle pain, and even rhabdomyolysis; while natural functional products have higher safety, but have problems such as low bioavailability and are difficult to be efficiently absorbed by the human body; at the same time, traditional probiotic products have single functions, mostly rely on single strains for preparation, have limited ability to regulate blood lipids, and have poor viability stability.

[0004] In view of the above related technologies, it is necessary to provide an auxiliary lipid-lowering composition that simultaneously has safety and high bioavailability. Summary of the Invention

[0005] In order to provide an auxiliary lipid-lowering composition that simultaneously has safety and high bioavailability, the present application provides an auxiliary lipid-lowering composition, a preparation method and an application thereof.

[0006] In a first aspect, a preparation method of an auxiliary lipid-lowering composition provided by the present application adopts the following technical solution:

[0007] A preparation method of an auxiliary lipid-lowering composition includes the following steps:

[0008] Step 1: First, wash and remove the cores of apples and red grapes, make them into pulp, and respectively crush white lentils, euryale ferox seeds, and lilies; then mix apples, white lentils, red grapes, euryale ferox seeds, and lilies in a mass ratio of (25-30):(20-25):(15-20):(10-15):(5-10), and then add 2 times the mass of water to make a uniform slurry, sterilize it to obtain a fermentation substrate;

[0009] Step 2: Take the fermentation substrate, inoculate Trichoderma reesei, and ferment under aerobic conditions at 28 - 30 °C for 30 - 36 h to obtain the initial fermentation product; Step 3: Take the initial fermentation product, after pasteurization, inoculate Candida krusei, and ferment under aerobic conditions at 28 - 30 °C for 20 - 24 h to obtain the second fermentation product;

[0010] Step 4: Take the second fermentation product, centrifuge and retain the supernatant, inoculate Bifidobacterium breve, and ferment under a nitrogen atmosphere at 35 - 37 °C for 30 - 32 h to obtain the third fermentation product;

[0011] Step 5: Take the third fermentation product, filter, sterilize, and then freeze-dry to obtain the fermentation product for assisting in reducing blood lipid;

[0012] Step 6: Mix the fermentation product for assisting in reducing blood lipid, freeze-dried natto powder, and ginkgo leaf extract evenly in a mass ratio of (2 - 4):(3 - 5):(0.5 - 1.5) to obtain the composition for assisting in reducing blood lipid.

[0013] In the above technical solution, apples and red grapes provide polyphenolic substances, white lentils and Gorgon euryale seeds are rich in resistant starch and dietary fiber, and lily contains saponin and flavonoid active ingredients. These raw materials act synergistically to form a fermentation substrate rich in nutrients.

[0014] Furthermore, in this application, through the step-by-step synergistic fermentation of Trichoderma reesei, Candida krusei, and Bifidobacterium breve, the active ingredients in the fermentation substrate are gradually released and transformed. First, nattokinase and others contained in Trichoderma reesei and freeze-dried natto powder interact with each other to decompose cellulose, hemicellulose, etc. in the raw materials, providing a fermentation product that is easier to decompose and transform for subsequent fermentation. Then, Candida krusei generates ethanol, ester flavor substances, and functional secondary metabolites and inhibits miscellaneous bacteria. Finally, Bifidobacterium breve promotes the production of more metabolites. In this application, through a specific fermentation substrate and three-step fermentation, metabolites that can inhibit cholesterol synthesis are generated, thus obtaining a fermentation product for assisting in reducing blood lipid with good cholesterol synthesis inhibitory ability.

[0015] Furthermore, in this application, by adding freeze-dried natto powder and ginkgo leaf extract, the freeze-dried natto powder contains rich vitamin K2 and nattokinase, and the ginkgo leaf extract is rich in active ingredients such as ginkgolide and bilobalide, which have multiple pharmacological effects such as antioxidant, anti-inflammatory, and antiplatelet aggregation. By compounding the freeze-dried natto powder, ginkgo leaf extract, and the fermentation product for assisting in reducing blood lipid, the blood lipid-lowering effect of the composition can be further enhanced. Therefore, the composition for assisting in reducing blood lipid of this application not only has a variety of natural active ingredients, but also improves the bioavailability through microbial fermentation, thus achieving an efficient and safe blood lipid-lowering effect.

[0016] Preferably, the inoculation amount of Trichoderma reesei in Step 2 is 5% - 8% of the total mass of the fermentation substrate.

[0017] In the above technical scheme, the present application limits the inoculation amount of Trichoderma reesei to 5% to 8% of the total mass of the fermentation substrate, thereby ensuring that Trichoderma reesei and freeze-dried natto powder have a better synergistic effect, and ensuring that the mycelium colonizes quickly and decomposes the fermentation substrate efficiently.

[0018] Preferably, the inoculation amount of Candida krusei in step 3 is 3% to 5% of the initial fermentation material mass.

[0019] In the above technical scheme, the present application limits the inoculation amount of Candida krusei to 3% to 5% of the mass of the initial fermentation material, thereby ensuring that Candida krusei occupies a dominant position in the fermentation process, effectively inhibiting the growth of miscellaneous bacteria, and generating more functional secondary metabolites.

[0020] Preferably, the inoculation amount of Bifidobacterium breve in step 4 is 5% to 8% of the mass of the supernatant.

[0021] In the above technical scheme, the present application limits the inoculation amount of Bifidobacterium breve to 5% to 8% of the mass of the supernatant, thereby ensuring that Bifidobacterium breve fully utilizes the nutrients in the supernatant for fermentation, promotes the production of more metabolites, and further enhances the auxiliary lipid-lowering effect.

[0022] Preferably, the sterilization in step 1 refers to high temperature sterilization at 121°C for 20 min, the pasteurization in step 3 refers to water bath sterilization at 65°C for 10 min, and the sterilization in step 5 refers to water bath sterilization at 65°C for 30 min.

[0023] In the above technical scheme, the present application can ensure the activity and safety of microorganisms during the fermentation process while avoiding the loss of nutrients through different sterilization steps and time control.

[0024] In the second aspect, the present application provides an auxiliary lipid-lowering composition using the following technical solution:

[0025] A composition for assisting in lowering blood lipids is prepared by the preparation method of the composition for assisting in lowering blood lipids as described in the first aspect.

[0026] In the above technical scheme, the present application discloses an auxiliary lipid-lowering composition prepared by the above preparation method. The composition is rich in polyphenols, resistant starch, dietary fiber, flavonoid active ingredients and metabolites, etc. These ingredients work synergistically to effectively inhibit cholesterol synthesis and lower blood lipid levels.

[0027] In summary, this application includes the following beneficial technical effects:

[0028] The auxiliary lipid-lowering composition provided by the present application and its preparation method not only solve the problems of large side effects and low bioavailability existing in lipid-lowering means in the prior art, but also generate metabolites that can inhibit cholesterol synthesis through specific fermentation substrates and stepwise co-fermentation technology, thereby achieving a safe and efficient lipid-lowering effect. Detailed implementation mode

[0029] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0030] Example 1: A preparation method of an auxiliary lipid-lowering composition, comprising the following steps:

[0031] Step 1: First, wash and remove the cores of apples and red grapes respectively, make them into pulp, and crush white lentils, euryale ferox seeds and lilies respectively; then mix apples, white lentils, red grapes, euryale ferox seeds and lilies in a mass ratio of 25:20:15:10:5, and then add 2 times the mass of water to make a uniform slurry, and sterilize it at 121°C for 20 min to obtain a fermentation substrate.

[0032] Step 2: Take the fermentation substrate and inoculate Trichoderma reesei. The inoculation amount of Trichoderma reesei is 5% of the total mass of the fermentation substrate, and ferment it under aerobic conditions at 28°C for 30 h to obtain an initial fermentation product.

[0033] Step 3: Take the initial fermentation product, sterilize it in a water bath at 65°C for 10 min, then inoculate Candida krusei. The inoculation amount of Candida krusei is 3% of the mass of the initial fermentation product, and ferment it under aerobic conditions at 28°C for 20 h to obtain a second fermentation product.

[0034] Step 4: Take the second fermentation product, centrifuge it and retain the supernatant, then inoculate Bifidobacterium breve. The inoculation amount of Bifidobacterium breve is 5% of the mass of the supernatant, and ferment it in a nitrogen atmosphere at 35°C for 30 h to obtain a third fermentation product.

[0035] Step 5: Take the third fermentation product, filter it, sterilize it in a water bath at 65°C for 30 min, and then freeze-dry it to obtain an auxiliary lipid-lowering fermented product.

[0036] Step 6: Mix the auxiliary lipid-lowering fermented product, freeze-dried natto powder and ginkgo leaf extract evenly in a mass ratio of 2:3:0.5 to obtain an auxiliary lipid-lowering composition.

[0037] Among them, the freeze-dried natto powder is a food-grade freeze-dried natto powder purchased from Zhengzhou Yuhe Food Additive Co., Ltd.

[0038] Among them, the ginkgo leaf extract is a food-grade ginkgo leaf extract purchased from Guangzhou Huayu Biotechnology Co., Ltd.

[0039] Among them, Trichoderma reesei is Trichoderma reesei CICC 13052, purchased from China Center of Industrial Culture Collection of Microorganisms.

[0040] Among them, Candida krusei is Candida krusei CICC 31807, purchased from China Center of Industrial Culture Collection of Microorganisms.

[0041] Among them, Bifidobacterium breve is Bifidobacterium breve CICC 6079, purchased from China Center of Industrial Culture Collection of Microorganisms.

[0042] Example 2: A method for preparing an auxiliary lipid-lowering composition, comprising the following steps:

[0043] Step 1: First, wash and pit apples and red grapes respectively, make them into pulp, and powder white lentils, Gorgon fruits, and lilies respectively; then mix apples, white lentils, red grapes, Gorgon fruits, and lilies according to a mass ratio of 28:22:18:12:7, and then add 2 times the mass of water to make a uniform slurry, and sterilize it at 121°C for 20 min to obtain a fermentation substrate.

[0044] Step 2: Take the fermentation substrate and inoculate Trichoderma reesei. The inoculation amount of Trichoderma reesei is 6% of the total mass of the fermentation substrate, and ferment it under aerobic conditions at 29°C for 32 h to obtain an initial fermentation product.

[0045] Step 3: Take the initial fermentation product, sterilize it in a water bath at 65°C for 10 min, then inoculate Candida krusei. The inoculation amount of Candida krusei is 4% of the mass of the initial fermentation product, and ferment it under aerobic conditions at 29°C for 22 h to obtain a second fermentation product.

[0046] Step 4: Take the second fermentation product, centrifuge it and retain the supernatant, inoculate Bifidobacterium breve. The inoculation amount of Bifidobacterium breve is 6% of the mass of the supernatant, and ferment it under a nitrogen atmosphere at 36°C for 31 h to obtain a third fermentation product.

[0047] Step 5: Take the third fermentation product, filter it, sterilize it in a water bath at 65°C for 30 min, and then freeze-dry it to obtain an auxiliary lipid-lowering fermented product.

[0048] Step 6: Mix the auxiliary lipid-lowering fermented product, freeze-dried natto powder, and ginkgo leaf extract evenly according to a mass ratio of 3:4:1 to obtain an auxiliary lipid-lowering composition.

[0049] Example 3: A method for preparing an auxiliary lipid-lowering composition, comprising the following steps:

[0050] Step 1: Wash apples and red grapes separately, remove the cores, make pulp, and separately crush white lentils, euryale seeds, and lilies. Then mix apples, white lentils, red grapes, euryale seeds, and lilies in a mass ratio of 30:25:20:15:10, and add 2 times the mass of water to make a uniform slurry. Sterilize at 121°C for 20 min to obtain a fermentation substrate.

[0051] Step 2: Take the fermentation substrate and inoculate Trichoderma reesei. The inoculation amount of Trichoderma reesei is 8% of the total mass of the fermentation substrate, and ferment under aerobic conditions at 30°C for 36 h to obtain an initial fermentation product.

[0052] Step 3: Take the initial fermentation product, sterilize it in a water bath at 65°C for 10 min, then inoculate Candida krusei. The inoculation amount of Candida krusei is 5% of the mass of the initial fermentation product, and ferment under aerobic conditions at 30°C for 24 h to obtain a second fermentation product.

[0053] Step 4: Take the second fermentation product, centrifuge and retain the supernatant, then inoculate Bifidobacterium breve. The inoculation amount of Bifidobacterium breve is 8% of the mass of the supernatant, and ferment in a nitrogen atmosphere at 37°C for 32 h to obtain a third fermentation product.

[0054] Step 5: Take the third fermentation product, filter it, sterilize it in a water bath at 65°C for 30 min, and then freeze-dry to obtain a fermentation product for assisting in reducing blood lipid.

[0055] Step 6: Mix the fermentation product for assisting in reducing blood lipid, freeze-dried natto powder, and ginkgo leaf extract evenly in a mass ratio of 4:5:1.5 to obtain a composition for assisting in reducing blood lipid.

[0056] Comparative Example 1: A preparation method of a composition for assisting in reducing blood lipid, which is different from Example 1 in that the fermentation sequence of the strains is inconsistent. The specific steps are as follows:

[0057] Step 1: Wash apples and red grapes separately, remove the cores, make pulp, and separately crush white lentils, euryale seeds, and lilies. Then mix apples, white lentils, red grapes, euryale seeds, and lilies in a mass ratio of 25:20:15:10:5, and add 2 times the mass of water to make a uniform slurry. Sterilize at 121°C for 20 min to obtain a fermentation substrate.

[0058] Step 2: Take the fermentation substrate and inoculate Candida krusei. The inoculation amount of Candida krusei is 3% of the mass of the fermentation substrate, and ferment under aerobic conditions at 28°C for 20 h to obtain an initial fermentation product.

[0059] Step 3: Take the initial fermentation product, sterilize it in a water bath at 65°C for 10 min, then inoculate Trichoderma reesei. The inoculation amount of Trichoderma reesei is 5% of the total mass of the initial fermentation product, and ferment under aerobic conditions at 28°C for 30 h to obtain a second fermentation product.

[0060] Step 4: Take the second fermentation product, centrifuge it and retain the supernatant. Inoculate Bifidobacterium breve, and the inoculation amount of Bifidobacterium breve is 5% of the mass of the supernatant. Ferment at 35°C in a nitrogen atmosphere for 30 h to obtain the third fermentation product.

[0061] Step 5: Take the third fermentation product, filter it, sterilize it in a water bath at 65°C for 30 min, and then freeze-dry it to obtain the fermentation product for assisting in reducing blood lipid.

[0062] Step 6: Mix the fermentation product for assisting in reducing blood lipid, freeze-dried natto powder, and ginkgo leaf extract evenly according to a mass ratio of 2:3:0.5 to obtain the composition for assisting in reducing blood lipid.

[0063] Comparative Example 2: A preparation method of a composition for assisting in reducing blood lipid, which is different from Example 1 in that Trichoderma reesei is not inoculated in Step 2, and specifically includes the following steps:

[0064] Step 1: First, wash and remove the cores of apples and red grapes respectively, make them into pulp, and crush white hyacinth beans, gorgon fruits, and lilies respectively; then mix apples, white hyacinth beans, red grapes, gorgon fruits, and lilies according to a mass ratio of 25:20:15:10:5, and then add 2 times the mass of water to make a uniform slurry, and sterilize it at 121°C for 20 min to obtain the fermentation substrate.

[0065] Step 2: Take the fermentation substrate and let it stand at 28°C under aerobic conditions for 30 h to obtain the initial fermentation product.

[0066] Step 3: Take the initial fermentation product, sterilize it in a water bath at 65°C for 10 min, and then inoculate Candida krusei. The inoculation amount of Candida krusei is 3% of the mass of the initial fermentation product, and ferment at 28°C under aerobic conditions for 20 h to obtain the second fermentation product.

[0067] Step 4: Take the second fermentation product, centrifuge it and retain the supernatant. Inoculate Bifidobacterium breve, and the inoculation amount of Bifidobacterium breve is 5% of the mass of the supernatant. Ferment at 35°C in a nitrogen atmosphere for 30 h to obtain the third fermentation product.

[0068] Step 5: Take the third fermentation product, filter it, sterilize it in a water bath at 65°C for 30 min, and then freeze-dry it to obtain the fermentation product for assisting in reducing blood lipid.

[0069] Step 6: Mix the fermentation product for assisting in reducing blood lipid, freeze-dried natto powder, and ginkgo leaf extract evenly according to a mass ratio of 2:3:0.5 to obtain the composition for assisting in reducing blood lipid.

[0070] Comparative Example 3: A preparation method of a composition for assisting in reducing blood lipid, which is different from Example 1 in that Candida krusei is not inoculated in Step 3, and specifically includes the following steps:

[0071] Step 1: Wash apples and red grapes separately, remove the cores, make pulp, and separately crush white lentils, euryale seeds, and lilies. Then mix apples, white lentils, red grapes, euryale seeds, and lilies in a mass ratio of 25:20:15:10:5, add 2 times the mass of water to make a uniform slurry, and sterilize at 121°C for 20 min to obtain a fermentation substrate.

[0072] Step 2: Take the fermentation substrate, inoculate Trichoderma reesei, and the inoculation amount of Trichoderma reesei is 5% of the total mass of the fermentation substrate. Ferment under aerobic conditions at 28°C for 30 h to obtain an initial fermentation product.

[0073] Step 3: Take the initial fermentation product, centrifuge and retain the supernatant, inoculate Bifidobacterium breve, and the inoculation amount of Bifidobacterium breve is 5% of the mass of the supernatant. Ferment in a nitrogen atmosphere at 35°C for 30 h to obtain a second fermentation product.

[0074] Step 4: Take the second fermentation product, filter, sterilize in a water bath at 65°C for 30 min, and then freeze-dry to obtain a fermentation product for assisting in reducing blood lipid.

[0075] Step 5: Mix the fermentation product for assisting in reducing blood lipid, freeze-dried natto powder, and ginkgo leaf extract evenly in a mass ratio of 2:3:0.5 to obtain a composition for assisting in reducing blood lipid.

[0076] Functional test for assisting in reducing blood lipid

[0077] Test samples: The compositions for assisting in reducing blood lipid in the above-mentioned examples and comparative examples.

[0078] Test animals, reagents, and feeding feeds: 70 SPF-grade male SD rats were provided by Dongchuang Experimental Animal Technology Service Department in Kaifu District, Changsha City. The experimental conditions were barrier environment. During the experiment, the experimental environmental temperature was 23°C - 24°C, and the humidity was 50% - 56%. Serum total cholesterol (TC) and triglyceride (TG) kits were purchased from Shanghai Fosun Changzheng Medical Science Co., Ltd., and high-density lipoprotein cholesterol (HDL-C) kits were purchased from Ningbo Meikang Biotech Co., Ltd. The feeding feed was a high-fat feed, and the formula was as follows: 8.8% basal feed, 1% cholesterol, 10% egg yolk powder, 10% lard, and 0.2% bile salt.

[0079] Test method: After feeding rats with basal diet for 7 days, fast them for 16 h, collect tail blood, and measure TC, TG and HDL-C. According to the levels of TC and TG, randomly divide the rats into 7 groups evenly, with 10 rats in each group. Among them, 6 groups are experimental groups and 1 group is the control group. Then start the formal experiment and continue to feed them with high-fat diet. After the formal experiment starts, the experimental groups are gavaged once a day with 200 mL of the test sample solution (16 g of the test sample + sodium carboxymethylcellulose solution made up to 200 mL), and the control group is gavaged once a day with 200 mL of 1% sodium carboxymethylcellulose solution. After 30 days of the formal experiment, fast them for 16 h, collect blood by puncturing the eyeballs to measure TC, TG and HDL-C. When the TC result of the experimental group is significant compared with that of the control group (P<0.05), and at the same time the HDL-C of the experimental group is significantly higher than that of the control group, it can be determined that the test sample has the function of assisting in reducing serum total cholesterol, and the result is positive. When the TG result of the experimental group is significant compared with that of the control group (P<0.05), and at the same time the HDL-C of the experimental group is significantly higher than that of the control group, it can be determined that the test sample has the function of assisting in reducing triglyceride, and the result is positive. When both TC and TG indicators are positive, it can be determined that the test sample has the function of assisting in reducing blood lipid.

[0080] The above test results are shown in Table 1.

[0081] Table 1:

[0082]

[0083] According to the data in Table 1, it can be seen that for the lipid-lowering assisting compositions prepared in Example 1, Example 2 and Example 3, in the rats after the test, both the TC level and the TG level are significantly decreased (P<0.05), and at the same time the HDL-C level is significantly increased (P<0.05). This indicates that the lipid-lowering assisting compositions in Example 1, Example 2 and Example 3 have the functions of assisting in reducing serum total cholesterol and triglyceride, and can significantly increase the high-density lipoprotein cholesterol level, thus having the effect of assisting in reducing blood lipid.

[0084] Specifically, in combination with Example 1 and Comparative Example 1, Comparative Example 2, and Comparative Example 3 for analysis, in Comparative Example 1, the fermentation order of the strains was changed, in Comparative Example 2, the inoculation step of Trichoderma reesei was omitted, and in Comparative Example 3, the inoculation step of Candida krusei was omitted. Only Example 1 achieved the expected lipid-lowering assisting effect. Thus, it can be seen that through the selection of fermentation strains and the setting of the fermentation order in this application, the obtained lipid-lowering assisting composition can significantly reduce the serum total cholesterol and triglyceride levels, and at the same time increase the high-density lipoprotein cholesterol level, thereby effectively assisting in reducing blood lipid.

[0085] This specific embodiment is only an interpretation of the present application and does not limit the present application. After reading this specification, those skilled in the art can make modifications to this embodiment without creative contributions as needed, but as long as it is within the scope of the claims of the present application, it is protected by the patent law.

Claims

1. A preparation method of an auxiliary lipid-lowering composition, characterized in that, It includes the following steps: Step 1: Wash and remove the cores of apples and red grapes respectively, make them into pulp, and crush white lentils, euryale ferox seeds, and lilies respectively; then mix apples, white lentils, red grapes, euryale ferox seeds, and lilies in a mass ratio of (25~30):(20~25):(15~20):(10~15):(5~10), add 2 times the mass of water to make a uniform slurry, sterilize it to obtain a fermentation substrate; Step 2: Take the fermentation substrate, inoculate Trichoderma reesei, and ferment it under aerobic conditions at 28~30°C for 30~36h to obtain an initial fermentation product; Step 3: Take the initial fermentation product, after pasteurization, inoculate Candida krusei, and ferment it under aerobic conditions at 28~30°C for 20~24h to obtain a second fermentation product; Step 4: Take the second fermentation product, centrifuge it and retain the supernatant, inoculate Bifidobacterium breve, and ferment it under a nitrogen atmosphere at 35~37°C for 30~32h to obtain a third fermentation product; Step 5: Take the third fermentation product, filter and sterilize it, and then freeze-dry it to obtain a fermentation product for assisting in reducing blood lipid; Step 6: Mix the fermentation product for assisting in reducing blood lipid, freeze-dried natto powder, and ginkgo biloba extract evenly in a mass ratio of (2~4):(3~5):(0.5~1.5) to obtain a composition for assisting in reducing blood lipid.

2. The preparation method of an auxiliary lipid-lowering composition according to claim 1, wherein, The inoculation amount of Trichoderma reesei in Step 2 is 5%~8% of the total mass of the fermentation substrate.

3. The preparation method of an auxiliary lipid-lowering composition according to claim 1, characterized in that, The inoculation amount of Candida krusei in Step 3 is 3%~5% of the mass of the initial fermentation product.

4. The preparation method of an auxiliary lipid-lowering composition according to claim 1, characterized in that, The inoculation amount of Bifidobacterium breve in Step 4 is 5%~8% of the mass of the supernatant.

5. The preparation method of an auxiliary lipid-lowering composition according to claim 1, characterized in that, The sterilization in Step 1 refers to high-temperature sterilization at 121°C for 20 min, the pasteurization in Step 3 refers to water bath sterilization at 65°C for 10 min, and the sterilization in Step 5 refers to water bath sterilization at 65°C for 30 min.

6. An auxiliary lipid-lowering composition, characterized in that, It is prepared by using the preparation method of the composition for assisting in reducing blood lipid according to any one of claims 1 to 5.

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