Detoxifying traditional Chinese medicine composition as well as preparation method and application thereof

Abstract

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for detoxifying, and a preparation method and application thereof. 20 + / -1 parts by mass of honeysuckle; 30 + / -1 parts by mass of dandelion; 30 + / -1 parts by mass of purslane; 10 + / -1 parts by mass of rheum officinale; 15 + / -1 parts by mass of hawthorn; 15 + / -1 parts by mass of rice sprouts; 15 + / -1 parts by mass of malt; 10 + / -1 parts by mass of licorice; 20 + / -1 parts by mass of plantain herb; 10 + / -1 parts by mass of platycodon grandiflorum; 10 + / -1 parts by mass of dried ginger; 10 + / -1 parts of herba cepbalanoplosis segeti; and 30 + / -1 parts by mass of lalang grass rhizome. The traditional Chinese medicine can effectively treat diseases caused by bee venom of bees, wasps, hornets, bamboo bees and the like, the detoxification effect is good, and severe scars such as hypertrophic scars and keloids are not prone to being generated after stings are healed; the traditional Chinese medicine composition has a certain anti-thrombus effect, and has a very good prevention effect on thrombus possibly caused by bee venom, so that the risk of thrombus formation is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a detoxifying traditional Chinese medicine composition, a preparation method and application thereof. Background Art

[0002] The types and composition of bee venom are very complex, and the venom composition of different species of bees is also different.

[0003] The main types of bee venom are:

[0004] 1. Bee venom: Bee venom is mainly an acidic liquid, containing formic acid and protein. Bee venom contains a variety of small molecular metabolic active substances, such as histamine, 5-hydroxytryptamine, dopamine, adrenaline, norepinephrine and acetylcholine. In addition, it also contains 10 types of bee venom peptides.

[0005] 2. Wasp venom: Wasp venom is usually weakly alkaline and contains complex toxins such as histamine, serotonin, bradykinin, etc. It has hemolytic, hemorrhagic and neurotoxic effects. Wasp venom contains wasp kinin.

[0006] 3. Wasp venom: Wasp venom also contains a variety of toxins, such as wasp kinin. Wasp venom is usually more toxic than honey bees, and its sting is not easy to fall off and can sting repeatedly.

[0007] 4. Parasitic wasp venom: The venom of parasitic wasps contains SPHs, neprilysin-like and cysteine-rich toxins, as well as some toxins unique to parasitic wasps, such as endocrine toxins. These toxins may suppress the host immune system during the parasitic process.

[0008] 5. Wasp and wasp-like venom: The venom of wasps (including yellow-waisted wasps, fire ants, etc.) contains a variety of peptide toxins, inflammatory peptides, anti-inflammatory peptides, analgesic peptides, anticoagulant peptides, neurotoxins, etc. These components have different biological activities in muscle damage, inflammation, cell lysis, etc.

[0009] 6. Bamboo bee venom: Bamboo bee venom is the most toxic, and its venom has a complex composition and may cause serious systemic reactions.

[0010] The components of different types of bee venom vary significantly, and these components can cause different physiological reactions in the human body, including local redness and swelling, pain, allergic reactions and even death.

[0011] Currently, the treatment for conventional bee venom (such as honey bees, hornets, wasps, etc.) is usually oral loratadine tablets, cetirizine hydrochloride tablets, acetaminophen tablets, supplemented with ibuprofen, etc., or supplemented with liver and kidney nourishing drugs, etc., and there is no specific medicine.

[0012] However, the damage caused by bee venom, if severe, can lead to deep wound ulceration. If the detoxification is not complete or the excess is not complete during treatment, it is easy to cause difficulty in skin healing, especially severe scars such as hypertrophic scars and keloids. In addition, for the most toxic bamboo bees, it is usually difficult to cure by only taking oral loratadine tablets, cetirizine hydrochloride tablets and acetaminophen tablets without other medical assistance.

[0013] Based on this, the present invention is proposed. Summary of the invention

[0014] The object of the present invention is to provide a detoxifying Chinese medicine composition, a preparation method and application thereof, so as to solve the above-mentioned problems.

[0015] In the first aspect, a detoxifying Chinese medicine composition is prepared from the following raw materials in parts by weight:

[0016] 15±1 parts by mass of dried tangerine peel; 20±1 parts by mass of honeysuckle; 30±1 parts by mass of dandelion; 30±1 parts by mass of purslane; 10±1 parts by mass of rhubarb; 15±1 parts by mass of hawthorn; 15±1 parts by mass of malt; 15±1 parts by mass of malt; 10±1 parts by mass of liquorice; 20±1 parts by mass of plantain; 10±1 parts by mass of platycodon; 10±1 parts by mass of dried ginger; 10±1 parts by mass of thistle; 30±1 parts by mass of Imperata root.

[0017] As a further improvement, the detoxifying Chinese medicine composition is prepared from the following raw materials in parts by weight:

[0018] 15 parts by weight of dried tangerine peel; 20 parts by weight of honeysuckle; 30 parts by weight of dandelion; 30 parts by weight of purslane; 10 parts by weight of rhubarb; 15 parts by weight of hawthorn; 15 parts by weight of malt; 15 parts by weight of malt; 10 parts by weight of licorice; 20 parts by weight of plantain; 10 parts by weight of platycodon; 10 parts by weight of dried ginger; 10 parts by weight of thistle; 30 parts by weight of Imperata root.

[0019] As a further improvement, the detoxifying Chinese medicine composition also includes pharmaceutically acceptable excipients.

[0020] As a further improvement, the pharmaceutical excipient is a pharmaceutically acceptable carrier, diluent or excipient.

[0021] In a second aspect, a method for preparing a detoxifying Chinese medicine composition comprises the following steps:

[0022] Step 1, according to the formula, weigh tangerine peel, honeysuckle, dandelion, purslane, rhubarb, hawthorn, malt sprout, malt, licorice, plantain, platycodon, dried ginger, thistle and white imperata root in sequence, and calculate the total weight of the above raw materials;

[0023] Step 2: Put all the above raw materials into a decoction bag, add purified water at a ratio of 5 to 6 times the total weight of the raw materials, and soak for 30 minutes;

[0024] Step 3: then heat to 100° C. in a medicine decoction machine, boil for 2 to 3 hours, filter after the decoction is completed, and obtain a Chinese medicine decoction.

[0025] A further improvement is to concentrate, spray dry and granulate the Chinese medicine decoction to prepare Chinese medicine granules;

[0026] and / or,

[0027] Concentrate, spray dry, granulate and tablet the Chinese medicine decoction to make Chinese medicine tablets;

[0028] and / or,

[0029] The Chinese medicine decoction is concentrated, spray-dried, granulated, and filled into capsule shells to prepare Chinese medicine capsules.

[0030] A third aspect is the use of a detoxifying Chinese medicine composition in the preparation of a drug for treating bee venom.

[0031] In a further improvement, the bee venom is one or more of honey bee toxin, wasp toxin, and wasp toxin.

[0032] A fourth aspect is the use of a detoxifying Chinese medicine composition in the preparation of a preventive / therapeutic antithrombotic drug.

[0033] A fifth aspect is the use of a detoxifying Chinese medicine composition in the preparation of medicines for treating bamboo bees.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] 1. The detoxifying Chinese medicinal composition of the present invention can effectively treat the diseases caused by bee venom, such as bees, hornets, and wasps, has good efficacy and detoxification effect, and the stung skin is not prone to produce severe scars such as hypertrophic scars and keloids after healing.

[0036] 2. The detoxifying Chinese medicinal composition of the present invention also has a certain anti-thrombotic effect and has a good preventive effect on thrombosis that may be caused by some bee venoms, thereby reducing the risk of thrombosis.

[0037] 3. The detoxifying Chinese medicinal composition of the present invention also has a certain detoxifying effect on bamboo bees that are particularly toxic, and the detoxifying effect is good. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 A chart of a patient's daily medical history;

[0039] Figure 2This is a diagram showing the test results on thrombosis in mice. DETAILED DESCRIPTION

[0040] The present invention is further described in detail below through specific embodiments in conjunction with the accompanying drawings.

[0041] Example 1

[0042] Recipe 1:

[0043] Tangerine peel 15g; honeysuckle 20g; dandelion 30g; purslane 30g; rhubarb 10g; hawthorn 15g; malt 15g; malt 15g; licorice 10g; plantain 20g; platycodon 10g; dried ginger 10g; thistle 10g; Imperata root 30g.

[0044] Decoction method:

[0045] Put all the above raw materials into a decoction bag, add 1300g of water, and soak for 30 minutes; then heat to 100°C in a decoction machine, boil for 2 hours and concentrate, filter after the decoction is completed, and obtain 60ml of Chinese medicine decoction.

[0046] The properties of the Chinese medicine composition (Chinese medicine decoction) obtained in Example 1 are explained from the following aspects.

[0047] 1. Pharmacological studies

[0048] In recipe 1, honeysuckle, dandelion and purslane are the main medicines, which can clear away heat, detoxify, reduce swelling and relieve stagnation; rhubarb, plantain, thistle and white imperata root can expel evil toxins through defecation and urine; hawthorn, tangerine peel, barley sprout, malt, platycodon and dried ginger can neutralize the cold medicine and protect the stomach qi; licorice can harmonize the various medicines.

[0049] 2. Clinical Observation

[0050] Clinical observation was conducted in the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine (Anhui Provincial Hospital of Traditional Chinese Medicine). A total of 60 patients from outpatient clinics and ICUs (29 with bee stings, 21 with wasp stings, and 10 with hornet stings) were selected and divided into two groups, a test group of 30 cases and a control group of 30 cases. The principle of randomization, parallel control, and double-blind was adopted.

[0051] The experimental group was treated orally with the Chinese medicine composition of Example 1, and the control group was treated with oral loratadine tablets (10 mg / day), cetirizine hydrochloride tablets (10 mg / day), and acetaminophen tablets (1.5 g / day). All 30 patients in the experimental group and 30 patients in the control group were cured and discharged after 7 days of treatment. There was no significant difference in the general data of the two groups of patients (P>0.05), and they were comparable.

[0052] The range of local reaction of sting is usually less than 10 cm. There were 21 cases of local wound ulceration with a diameter of more than 3 cm in the experimental group and 18 cases of local wound ulceration with a diameter of more than 3 cm in the control group. The ulcerated area was rinsed and disinfected with normal saline every day.

[0053] It has been found in medical treatment that if the bee sting is toxic, it will cause ulceration and necrosis in the area, and scars will form after healing in the later stage. If local malignant lesions occur, hypertrophic scars, keloids, etc. will occur.

[0054] Scars that are relatively flat locally and have a smooth surface are considered normal scars.

[0055] Hypertrophic scars are irregular in shape, protruding from the surface, and hard in texture. Hypertrophic scars are bright red, brown, etc. Hypertrophic scars may cause symptoms such as itching and stinging.

[0056] Keloids are round or oval protrusions with a smooth surface. The color of keloids is generally pink or dark red. Keloids can cause obvious pain, itching, and fever in the scar.

[0057] For the convenience of description, hypertrophic scars and keloids are collectively referred to as severe scars. It is well known in the art that hypertrophic scars and keloids should be taken seriously if they appear on the face, because if they are not properly cared for in the later stage, they will affect the patient's psychology. For example, hypertrophic scars can be treated with Centella asiatica cream ointment, scar repair cream or injection of glucocorticoids under the guidance of a doctor to fade. For keloids, they should be treated with halometasone ointment, compound heparin sodium allantoin gel or injection of Baosong liquid under the guidance of a doctor to fade.

[0058] Clinical observations revealed that:

[0059] After the patients were cured, only 1 of the 21 patients with local deep ulceration in the experimental group developed hypertrophic scars (wasp stings), and the incidence of severe scars = 4.8%. In the control group, 7 of the 18 patients with local deep ulceration eventually developed hypertrophic scars (4 were wasp stings, 3 were bee stings), and 2 had keloids (both were wasp stings), and the incidence of severe scars = 50%.

[0060] It can be seen from this that the Chinese medicine composition of the present invention can not only be used to treat patients with bee venom injuries, but also has good therapeutic effects, especially good detoxification effects, and can effectively reduce the probability of severe scarring in patients stung by bees, hornets and wasps after recovery.

[0061] 3. Clinical Cases

[0062] Patient Xu's daily medical records are available at Figure 1; He was injured by bee venom and was in the intensive care unit. During diagnosis and treatment, combined with the tongue and pulse, he was given an oral heat-clearing, detoxifying and diuretic agent. The prescription used honeysuckle, dandelion, and purslane to clear away heat and detoxify, reduce swelling and disperse nodules; rhubarb, plantain, thistle, and white grass root allowed the evil toxins to be excreted through the stool and urine; hawthorn, tangerine peel, barley sprout, malt, platycodon, and dried ginger neutralized the cooling medicine and protected the stomach qi; licorice harmonized the various medicines.

[0063] The Chinese medicine decoction prepared according to Example 1 (decocted in water) was taken once a day in 2 divided doses; taken for 5 consecutive days, the patient recovered and was discharged from the hospital.

[0064] IV. Effects of Thrombosis on Mice

[0065] 40 ICR mice weighing 25-30 g were selected and randomly divided into 4 groups: administration group 1: the Chinese medicine decoction prepared in Example 1 (3 ml of the Chinese medicine decoction was given by gavage every day); reference group: compound Danshen tablets (national medicine standard Z44020262, 500 mg / kg / day); administration group 2: loratadine tablets (1.2 mg / kg / day), cetirizine hydrochloride tablets (1.2 mg / kg / day), acetaminophen tablets (120 mg / kg / day); model group: an equal amount of normal saline.

[0066] Each group of mice was given the drug once a day by oral gavage according to the dose for 5 consecutive days. 30 minutes after the last administration, 250 mg / kg adenosine diphosphate was injected into the tail vein of the mice to establish the model. 15 minutes later, the number of surviving mice was recorded. The results are shown in Figure 2 As shown (Note: Compared with the model group, * P<0.05).

[0067] Depend on Figure 2 It can be seen that the drug administration group 1 and the reference group can significantly improve the survival rate of mice with ADP-induced pulmonary thrombosis, and it is well known in the art that the compound Danshen tablets used in the reference group are an antithrombotic agent; the antithrombotic efficacy of the Chinese medicine decoction prepared in Example 1 is about half that of the compound Danshen tablets. This has a good preventive effect on the thrombosis that may be caused by some bee venom, thereby reducing the risk of thrombosis.

[0068] 5. The therapeutic effect of Chinese herbal medicine combination on bamboo bee stings

[0069] Thirty ICR mice weighing 40-45 g were selected and randomly divided into three groups: administration group 3: the Chinese medicine decoction prepared in Example 1 (3 ml of the Chinese medicine decoction was given by gavage every day); administration group 4: loratadine tablets (1.2 mg / kg / day), cetirizine hydrochloride tablets (1.2 mg / kg / day), acetaminophen tablets (120 mg / kg / day); blank group: an equal amount of normal saline.

[0070] The mice in each group were subcutaneously injected with bamboo bee venom on their backs. The bamboo bee venom was extracted from the glands where the stingers of 20 bamboo bees were located on their abdomens and mixed with deionized water. The model was successfully established 4 hours after the injection of bamboo bee venom. The necrotic tissue was removed from the ulcers of all mice, the ulcers were cleaned, and the drugs were administered orally as required; the drugs were administered continuously for 10 days, and the unhealed area of ​​the ulcerated skin of the mice was measured on the 11th day; the results are shown in Table 1:

[0071] Table 1

[0072]

[0073] Since bamboo bee venom is the strongest venom among bee venoms, it is very difficult to cure. In the present invention, the detoxifying Chinese medicine composition obtained in Example 1 can effectively treat bamboo bee stings. However, the Western medicine group (loratadine tablets, cetirizine hydrochloride tablets, acetaminophen tablets) that is also effective for bee, hornet and wasp stings has limited therapeutic effect on bamboo bee stings.

[0074] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A detoxifying Chinese medicine composition, characterized in that: It is prepared from the following raw materials in parts by weight: 15±1 parts by mass of dried tangerine peel; 20±1 parts by mass of honeysuckle; 30±1 parts by mass of dandelion; 30±1 parts by mass of purslane; 10±1 parts by mass of rhubarb; 15±1 parts by mass of hawthorn; 15±1 parts by mass of malt; 15±1 parts by mass of malt; 10±1 parts by mass of liquorice; 20±1 parts by mass of plantain; 10±1 parts by mass of platycodon; 10±1 parts by mass of dried ginger; 10±1 parts by mass of thistle; 30±1 parts by mass of Imperata root.

2. A detoxifying Chinese medicine composition according to claim 1, characterized in that: It is prepared from the following raw materials in parts by weight: 15 parts by weight of dried orange peel; 20 parts by weight of honeysuckle; 30 parts by weight of dandelion; 30 parts by weight of purslane; 10 parts by weight of rhubarb; 15 parts by weight of hawthorn; 15 parts by weight of malt sprouts; 15 parts by weight of malt; 10 parts by weight of liquorice; 20 parts by weight of plantain; 10 parts by weight of Platycodon grandiflorum; 10 parts by weight of dried ginger; 10 parts by mass of thistle; 30 parts by mass of Imperata cylindrica.

3. A detoxifying Chinese medicine composition according to claim 1, characterized in that: Pharmaceutically acceptable excipients are also included.

4. A detoxifying Chinese medicine composition according to claim 3, characterized in that: The pharmaceutical excipients are pharmaceutically acceptable carriers, diluents and excipients.

5. A method for preparing a detoxifying Chinese medicine composition according to any one of claims 1 to 4, characterized in that: The following steps are involved: Step 1, according to the formula, weigh tangerine peel, honeysuckle, dandelion, purslane, rhubarb, hawthorn, malt sprout, malt, licorice, plantain, platycodon, dried ginger, thistle and white imperata root in sequence, and calculate the total weight of the above raw materials; Step 2: Put all the above raw materials into a decoction bag, add purified water at a ratio of 5 to 6 times the total weight of the raw materials, and soak for 30 minutes; Step 3: then heat to 100° C. in a medicine decoction machine, boil for 2 to 3 hours, filter after the decoction is completed, and obtain a Chinese medicine decoction.

6. The method for preparing a detoxifying Chinese medicine composition according to claim 5, characterized in that: Concentrate, spray dry and granulate the Chinese medicine decoction to make Chinese medicine granules; and / or, Concentrate, spray dry, granulate and tablet the Chinese medicine decoction to make Chinese medicine tablets; and / or, The Chinese medicine decoction is concentrated, spray-dried, granulated, and filled into capsule shells to prepare Chinese medicine capsules.

7. Use of the detoxifying Chinese medicinal composition as claimed in claim 1 or 2 in the preparation of a drug for treating bee venom.

8. Use of a detoxifying Chinese medicinal composition according to claim 7 in the preparation of a drug for treating bee venom, characterized in that: The bee venom is one or more of honey bee toxin, wasp toxin and wasp toxin.

9. Use of a detoxifying Chinese medicine composition as claimed in claim 1 or 2 in the preparation of a preventive / therapeutic antithrombotic drug.

10. Use of a detoxifying Chinese medicinal composition as claimed in claim 1 or 2 in preparing medicine for treating bamboo bees.

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