A detoxified traditional Chinese medicine composition, a preparation method and application thereof

Abstract

The application belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for detoxification, a preparation method and application thereof, which comprises the following components in parts by mass: 15+ / -1 parts of pericarpium citri reticulatae; 20+ / -1 parts of honeysuckle; 30+ / -1 parts of herba taraxaci; 30+ / -1 parts of herba portulacae; 10+ / -1 parts of radix et rhizoma rhei; 15+ / -1 parts of fructus crataegi; 15+ / -1 parts of fructus avenae; 15+ / -1 parts of fructus avenae; 10+ / -1 parts of radix glycyrrhizae; 20+ / -1 parts of herba plantaginis; 10+ / -1 parts of radix platycodi; 10+ / -1 parts of zingiber officinale; 10+ / -1 parts of herba eriodes; and 30+ / -1 parts of rhizoma imperatae. The application can effectively treat diseases caused by bee venom of bees, hornets, wasps and bamboo bees, has good detoxification effect, and can prevent some thrombosis caused by bee venom, thereby reducing the risk of thrombosis.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for detoxification, a preparation method and application thereof. BACKGROUND

[0002] The types and components of bee venom are very complex, and the venom components of different types of bees are also different.

[0003] The following are several main types of bee venom:

[0004] 1. Honeybee venom: Honeybee venom is mainly acidic liquid, containing formic acid and protein components. Honeybee venom contains a variety of small molecule metabolically active substances, such as histamine, 5-hydroxytryptamine, dopamine, adrenaline, norepinephrine and acetylcholine, etc. In addition, it contains 10 kinds of bee venom peptides.

[0005] 2. Wasp venom: Wasp venom is usually weakly alkaline, containing histamine, serotonin, bradykinin and other complex toxins, which have hemolytic, hemorrhagic and neurotoxic effects. Wasp venom contains wasp kinin.

[0006] 3. Yellow jacket venom: Yellow jacket venom also contains a variety of toxins, such as yellow jacket kinin. The toxicity of yellow jacket is usually stronger than that of honeybee, and its sting is not easy to fall off, which can repeatedly sting.

[0007] 4. Parasitic bee venom: The venom of parasitic bee contains SPHs, neprilysin-like and cysteine-rich toxins, and some unique toxins of parasitic bee, such as endothelin. These toxins may have inhibitory effect on the host immune system during parasitization.

[0008] 5. Yellow hornet and wasp venom: The venom of yellow hornet (including yellow waist hornet, fire ant, etc.) contains a variety of peptide toxins, inflammatory peptides, anti-inflammatory peptides, analgesic peptides, anticoagulant peptides, neurotoxins, etc. These components have different biological activities in muscle damage, inflammation, cell lysis, etc.

[0009] 6. Bamboo bee venom: Bamboo bee has the strongest toxicity, and its venom components are complex, which can cause severe systemic reactions.

[0010] The components of different types of bee venom are significantly different, and these components can cause different physiological responses in the human body, including local redness, pain, allergic reactions and even death.

[0011] At present, the treatment of conventional bee venom (such as honeybee, wasp, yellow jacket, etc.) usually uses oral chlorpheniramine maleate tablets, cetirizine hydrochloride tablets, acetaminophen tablets, and is supplemented with ibuprofen, etc., or is supplemented with liver-protecting and kidney-protecting drugs, and there is no specific drug.

[0012] However, the damage caused by bee venom, if serious, can cause deep ulceration of the wound, and if detoxification is not complete or residual during treatment, it is easy to cause difficulty in skin healing, especially the formation of serious scars such as hypertrophic scars and keloids. In addition, for the most toxic bamboo bees, it is usually difficult to cure only by oral administration of loratadine tablets, cetirizine hydrochloride tablets and acetaminophen tablets, without the aid of other medical means.

[0013] Based on this, the present application is proposed. SUMMARY

[0014] The purpose of the present application is to provide a detoxifying traditional Chinese medicine composition, a preparation method and its application to solve the above problems.

[0015] In a first aspect, a detoxifying traditional Chinese medicine composition is prepared from the following raw materials by weight:

[0016] 15±1 parts by mass of dried tangerine or orange peel; 20±1 parts by mass of honeysuckle; 30±1 parts by mass of dandelion; 30±1 parts by mass of purslane; 10±1 parts by mass of rhubarb; 15±1 parts by mass of hawthorn; 15±1 parts by mass of wheat germ; 15±1 parts by mass of barley; 10±1 parts by mass of licorice; 20±1 parts by mass of plantain; 10±1 parts by mass of platycodon grandiflorum; 10±1 parts by mass of dried ginger; 10±1 parts by mass of small chrysanthemum; and 30±1 parts by mass of white flag.

[0017] Further improvement, the detoxifying traditional Chinese medicine composition is prepared from the following raw materials by weight:

[0018] 15 parts by mass of dried tangerine or orange peel; 20 parts by mass of honeysuckle; 30 parts by mass of dandelion; 30 parts by mass of purslane; 10 parts by mass of rhubarb; 15 parts by mass of hawthorn; 15 parts by mass of wheat germ; 15 parts by mass of barley; 10 parts by mass of licorice; 20 parts by mass of plantain; 10 parts by mass of platycodon grandiflorum; 10 parts by mass of dried ginger; 10 parts by mass of small chrysanthemum; and 30 parts by mass of white flag.

[0019] Further improvement, the detoxifying traditional Chinese medicine composition further comprises a pharmaceutically acceptable pharmaceutical excipient.

[0020] Further improvement, the pharmaceutical excipient is a pharmaceutically acceptable carrier, diluent, excipient.

[0021] In a second aspect, a preparation method of a detoxifying traditional Chinese medicine composition is provided, comprising the following steps:

[0022] Step 1, according to the formula, dried tangerine or orange peel, honeysuckle, dandelion, purslane, rhubarb, hawthorn, wheat germ, barley, licorice, plantain, platycodon grandiflorum, dried ginger, small chrysanthemum and white flag are weighed in turn, and the total weight of the above raw materials is calculated;

[0023] Step 2, all the above raw materials are put into a decoction bag, purified water is added in a proportion of 5-6 times of the total weight of the raw materials, and soaked for 30 minutes;

[0024] Step 3, then heated to 100 DEG C in the decoction machine, decoct for 2-3 hours, after the decoction is finished, filter, get traditional Chinese medicine decoction.

[0025] Further improvement, the traditional Chinese medicine decoction is concentrated, spray dried, granulated, and made into traditional Chinese medicine granules;

[0026] And / or,

[0027] The traditional Chinese medicine decoction is concentrated, spray dried, granulated, and made into traditional Chinese medicine tablets;

[0028] And / or,

[0029] The traditional Chinese medicine decoction is concentrated, spray dried, granulated, and filled into a capsule shell to make a traditional Chinese medicine capsule.

[0030] The third aspect is the application of the detoxified traditional Chinese medicine composition in the preparation of a medicine for treating bee venom.

[0031] Further improvement, the bee venom is one or more of honeybee venom, hornet venom and wasp venom.

[0032] The fourth aspect is the application of the detoxified traditional Chinese medicine composition in the preparation of a medicine for preventing / treating antithrombotic drugs.

[0033] The fifth aspect is the application of the detoxified traditional Chinese medicine composition in the preparation of a medicine for treating bamboo bees.

[0034] Compared with the prior art, the present application has the following advantages:

[0035] 1. The detoxified traditional Chinese medicine composition can effectively treat the diseases caused by bee venom, hornet venom and wasp venom, has good efficacy and detoxification effect, and the skin after healing is not easy to produce serious scars such as hypertrophic scars and keloids.

[0036] 2. The detoxified traditional Chinese medicine composition also has certain antithrombotic effect, has good preventive effect on thrombosis caused by some bee venom, thereby reducing the risk of thrombosis.

[0037] 3. The detoxified traditional Chinese medicine composition also has certain detoxification effect on bamboo bees with extremely strong toxicity, and has good detoxification effect. BRIEF DESCRIPTION OF DRAWINGS

[0038] Figure 1 It is a daily course record chart for a patient;

[0039] Figure 2The results of the test on the influence of thrombus formation in mice are shown in the following graph. DETAILED DESCRIPTION

[0040] The application will be further described in detail below with specific examples and in conjunction with the accompanying drawings.

[0041] Example 1

[0042] Formula 1:

[0043] Dried tangerine peel 15g; honeysuckle 20g; dandelion 30g; purslane 30g; rhubarb 10g; hawthorn 15g; wheat germ 15g; licorice 10g; plantain 20g; platycodon 10g; dried ginger 10g; small chrysanthemum 10g; white bamboo root 30g.

[0044] Water decoction method:

[0045] Put all the above raw materials into the decoction bag, add 1300g of water, soak for 30 minutes; then heat to 100℃ in the decoction machine, concentrate after decocting for 2 hours, filter after the decoction is finished, and get 60ml of traditional Chinese medicine decoction.

[0046] The properties of the traditional Chinese medicine composition (traditional Chinese medicine decoction) obtained in Example 1 are described from the following aspects.

[0047] I. Pharmacological study

[0048] In the formula of Formula 1, honeysuckle, dandelion and purslane are the monarch drugs, which clear heat and detoxify, and dissipate stasis and resolve mass; rhubarb, plantain, small chrysanthemum and white bamboo root expel evil toxins from the two excretory systems; hawthorn, dried tangerine peel, wheat germ, licorice, platycodon and dried ginger are the neutral and cooling drugs that protect the stomach qi; and licorice harmonizes all the drugs.

[0049] II. Clinical observation

[0050] Clinical observation was carried out in the First Affiliated Hospital of Anhui University of Chinese Medicine (Anhui Provincial Hospital of Traditional Chinese Medicine), and a total of 60 outpatients and ICU patients (29 cases of bee stings, 21 cases of wasp stings and 10 cases of wasp stings) were selected and divided into two groups, 30 cases in the test group and 30 cases in the control group. The principles of randomization, parallel control and double blinding were adopted.

[0051] The test group was treated orally with the traditional Chinese medicine composition of Example 1, and the control group was treated orally with loratadine tablets (10mg / day), cetirizine hydrochloride tablets (10mg / day) and acetaminophen tablets (1.5g / day). After 7 days of treatment, all 30 patients in the test group and all 30 patients in the control group were cured and discharged. There was no statistically significant difference (P>0.05) in the comparison of the general data of the patients in the two groups, and they were comparable.

[0052] The range of local reaction of the sting is usually less than 10 cm, 21 cases of local wound ulceration and ulceration area diameter greater than 3 cm appear in the test group, and 18 cases of local wound ulceration and ulceration area diameter greater than 3 cm appear in the control group. The ulceration area is washed and disinfected with normal saline every day.

[0053] It is found in medical treatment that if the toxicity is relatively serious after the bee sting, the part will cause ulceration, necrosis and the like, and after healing in the later period, a scar will be formed. If local malignant change occurs, a hypertrophic scar, a keloid and the like will be produced.

[0054] The local part is relatively flat, the surface is smooth and the like, which belongs to a normal scar.

[0055] The shape is irregular, protrudes from the surface, the texture is hard and the like, which belongs to a hypertrophic scar. The color of the hypertrophic scar is bright red, brown and the like, and the hypertrophic scar can cause symptoms such as itching and stinging pain,

[0056] The keloid is a circular or elliptical protrusion, and the surface is smooth. The color of the keloid is generally pink or dark red. The keloid can cause symptoms such as obvious pain, itching and fever at the scar site.

[0057] For the convenience of description, the hypertrophic scar and the keloid are collectively referred to as a serious scar. It is known in the art that if the hypertrophic scar and the keloid appear on the face, attention should be paid because if the care is not proper in the later period, the patient's psychology will be affected. For example, the hypertrophic scar can be treated to be faded by using the asiaticoside cream ointment, the scar repair cream or the injection of glucocorticoid under the guidance of a doctor. For example, the keloid should be treated to be faded by using the halometasone ointment, the compound heparin sodium urokinase gel or the injection of baosong liquid under the guidance of a doctor.

[0058] It is found in clinical observation that:

[0059] After the patient is cured, only one case of the hypertrophic scar (Hu Feng sting) appears in the 21 cases of local deep ulceration patients in the test group, and the serious scar appearance rate is 4.8%. In the 18 cases of local deep ulceration patients in the control group, 7 cases of the hypertrophic scar (4 cases of Hu Feng sting and 3 cases of bee sting) and 2 cases of the keloid (both of which are hornet stings) appear, and the serious scar appearance rate is 50%.

[0060] Therefore, it can be known that the traditional Chinese medicine composition can be used for treating the peak toxin injury patient, has good treatment effect, especially good detoxification effect, and can effectively reduce the probability of the serious scar of the bee, Hu Feng and hornet sting patient after healing.

[0061] III. Clinical cases

[0062] The daily course record of the patient Xu is as follows: Figure 1The patient was injured by bee venom and was in the intensive care unit. During diagnosis and treatment, based on the tongue and pulse, a heat-clearing, detoxifying, and diuretic formula was administered orally. The formula included honeysuckle, dandelion, and purslane to clear heat, detoxify, reduce swelling, and dissipate nodules; rhubarb, plantain, small thistle, and imperata root to expel pathogenic toxins through urination and defecation; hawthorn, tangerine peel, germinated barley, malt, platycodon, and dried ginger to neutralize the cooling herbs and protect the stomach qi; and licorice to harmonize the various herbs.

[0063] The traditional Chinese medicine decoction prepared according to Example 1 (decocted in water) is taken once a day in two divided doses; after taking it for 5 consecutive days, the patient is discharged from the hospital after recovery.

[0064] IV. Effects on thrombosis in mice (test)

[0065] Forty ICR mice, weighing 25–30 g, were randomly divided into four groups: Group 1: Traditional Chinese medicine decoction prepared in Example 1 (3 ml of the decoction was administered by gavage daily); Control group: Compound Danshen tablets (National Drug Approval Number Z44020262, 500 mg / kg / day); Group 2: Loratadine tablets (1.2 mg / kg / day), Cetirizine hydrochloride tablets (1.2 mg / kg / day), Acetaminophen tablets (120 mg / kg / day); Model group: Equal volume of physiological saline.

[0066] Mice in each group were administered the drug orally once daily for 5 consecutive days. Thirty minutes after the last administration, adenosine diphosphate (ATP) was injected intravenously at a dose of 250 mg / kg to induce a mouse model. The number of surviving mice was recorded 15 minutes later. Results are as follows: Figure 2 As shown (Note: compared with the model group, * P < 0.05.

[0067] Depend on Figure 2 It was found that the treatment group 1 and the control group significantly improved the survival rate of mice with ADP-induced pulmonary thrombosis. It is well known in the art that the compound Danshen tablets used in the control group are an antithrombotic agent. The traditional Chinese medicine decoction prepared in Example 1 has approximately half the antithrombotic efficacy of the compound Danshen tablets. This has a good preventive effect against thrombosis that may be caused by bee venom, thereby reducing the risk of thrombosis.

[0068] V. The therapeutic effect of traditional Chinese medicine combination on bamboo bee stings

[0069] Thirty ICR mice, weighing 40–45 g, were randomly divided into three groups: Group 3: Traditional Chinese medicine decoction prepared in Example 1 (3 ml of the decoction was administered by gavage daily); Group 4: Loratadine tablets (1.2 mg / kg / day), cetirizine hydrochloride tablets (1.2 mg / kg / day), and acetaminophen tablets (120 mg / kg / day); Blank group: an equal volume of physiological saline.

[0070] The mice in each group were subcutaneously injected with the wasp venom on the back, and the wasp venom was extracted from the glands of 20 wasps and mixed with deionized water. The modeling was successful 4 hours after the injection of the wasp venom. The necrotic tissue was removed from the ulcer mouth of all the mice, the ulcer mouth was cleaned, and the mice were orally administered according to the requirements; the administration was continuously performed for 10 days, and the unhealed area of the ulcer skin of the mice was measured on the 11th day; the results are shown in Table 1:

[0071] Table 1

[0072]

[0073] Since the wasp venom is the strongest venom in the bee venom, it is very difficult to cure. In the present application, the detoxified traditional Chinese medicine composition obtained in Example 1 can effectively treat the wasp stings. However, the western medicine group (chlorpheniramine maleate tablets, cetirizine hydrochloride tablets, and acetaminophen tablets) which is also effective for the stings of bees, hornets and wasps has limited effect on the treatment of hornet stings.

[0074] The above only provides the preferred embodiments of the present application and is not used to limit the present application. The present application can have various modifications and changes for those skilled in the art. Any modification, equivalent replacement, improvement, etc. within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A traditional Chinese medicine composition for treating bee venom, characterized in that, It is prepared from the following raw materials in parts by weight: 14-16 parts by weight of dried tangerine peel, 19-21 parts by weight of honeysuckle, 29-31 parts by weight of dandelion, 29-31 parts by weight of purslane, 9-11 parts by weight of rhubarb, 14-16 parts by weight of hawthorn, 14-16 parts by weight of germinated barley, 14-16 parts by weight of malt, 9-11 parts by weight of licorice, 19-21 parts by weight of plantain, 9-11 parts by weight of platycodon, 9-11 parts by weight of dried ginger, 9-11 parts by weight of small thistle, and 29-31 parts by weight of Imperata cylindrica root.

2. The traditional Chinese medicine composition for treating bee venom according to claim 1, characterized in that, It is prepared from the following raw materials in parts by weight: 15 parts by weight of dried tangerine peel, 20 parts by weight of honeysuckle, 30 parts by weight of dandelion, 30 parts by weight of purslane, 10 parts by weight of rhubarb, 15 parts by weight of hawthorn, 15 parts by weight of germinated barley, 15 parts by weight of malt, 10 parts by weight of licorice, 20 parts by weight of plantain, 10 parts by weight of platycodon, 10 parts by weight of dried ginger, 10 parts by weight of small thistle, and 30 parts by weight of Imperata cylindrica root.

3. The traditional Chinese medicine composition for treating bee venom according to claim 1, characterized in that: It also includes pharmaceutically acceptable excipients.

4. The traditional Chinese medicine composition for treating bee venom according to claim 3, characterized in that: The pharmaceutical excipients are pharmaceutically acceptable carriers, diluents, and excipients.

5. A method for preparing a traditional Chinese medicine composition for treating bee venom as described in any one of claims 1 to 4, characterized in that, Includes the following steps: Step 1: Weigh out the following ingredients in order according to the recipe: dried tangerine peel, honeysuckle, dandelion, purslane, rhubarb, hawthorn, germinated rice, malt, licorice, plantain, platycodon, dried ginger, small thistle, and imperata root, and calculate the total weight of the above ingredients. Step 2: Put all the above ingredients into a decoction bag, add purified water at a ratio of 5 to 6 times the total weight of the ingredients, and soak for 30 minutes; Step 3: Then heat the decoction to 100°C in the decoction machine and decoct for 2-3 hours. After decoction, filter to obtain the Chinese herbal decoction.

6. The method for preparing a traditional Chinese medicine composition for treating bee venom according to claim 5, characterized in that: Traditional Chinese medicine decoctions are concentrated, spray-dried, and granulated to produce traditional Chinese medicine granules. And / or, Traditional Chinese medicine decoctions are concentrated, spray-dried, granulated, and compressed into tablets to produce traditional Chinese medicine tablets. And / or, Traditional Chinese medicine decoctions are concentrated, spray-dried, granulated, and filled into capsule shells to produce traditional Chinese medicine capsules.

7. The use of the traditional Chinese medicine composition for treating bee venom as described in claim 1 or 2 in the preparation of a drug for treating bee venom.

8. The application of the traditional Chinese medicine composition for treating bee venom according to claim 7 in the preparation of a drug for treating bee venom, characterized in that: The bee venom is one or more of the following: bee venom, wasp venom, and hornet venom.

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