A method for accurately covering a sealing film of a 96-well microplate

Abstract

The present invention relates to the technical field of 96-well microplate sealing film, specifically a method for accurately pasting the sealing film of a 96-well microplate, the method comprising the following steps: placing the 96-well microplate in the positioning fixture hole; arranging the sealing film on the positioning fixture seat, and embedding the positioning convex piece of the sealing film into the positioning notch to facilitate the rapid and accurate positioning of the sealing film; using a scraper to scrape or a roller to roll the sealing film, so that the sealing film fits tightly on the positioning fixture seat. The present invention not only improves the work efficiency when the fixture is positioned to paste the film, but also improves the pasting effect and accuracy of the sealing film.

Description

Technical Field

[0001] The invention relates to the technical field of jigs, and in particular to a method for accurately pasting a sealing film of a 96-well microplate. Background Art

[0002] With the rapid development of biotechnology and medical diagnostic technology, PCR (polymerase chain reaction) technology has become an indispensable core technical means in many key fields such as disease diagnosis, gene testing, virus screening, etc. As the key carrier for carrying test samples, the sealing of 96-well microplate is directly related to the accuracy of the test results and the safety and stability of the samples themselves.

[0003] In the current actual production and application scenarios, the lamination operation of the 96-well microplate sealing film mostly relies on manual operation. The operator holds the sealing film and tries to fit the film to the surface of the test box based on visual observation and experience. This traditional method has many disadvantages: on the one hand, it is difficult to accurately control the position of the sealing film by manual operation, and it is very easy to have offsets, wrinkles and other conditions, which makes it impossible for the sealing film to tightly cover the opening of the test box, creating opportunities for external contaminants to invade, seriously threatening the purity of the sample and increasing the risk of misjudgment of the test results; on the other hand, the efficiency of manual lamination is extremely low. In the face of large-scale sample testing needs, it consumes a lot of manpower and time costs, becoming a bottleneck factor restricting the improvement of detection efficiency. In addition, there are differences in the techniques of different operators, and it is difficult to ensure the consistency of the sealing film lamination quality during mass production, which is not conducive to the implementation of standardized operating procedures and quality management.

[0004] In view of this, it is urgent to develop a technical means to achieve accurate and efficient coating of 96-well microplate sealing membrane. This is not only related to the detection quality and efficiency in the field of medical testing, but also the key to ensuring the safety of biological samples and promoting the steady development of the industry. Summary of the invention

[0005] The object of the present invention is to provide a method for accurately covering a sealing film of a 96-well microplate, so as to solve the problems in the above-mentioned background technology.

[0006] 96-well microplates include deep-well plates, two-color well plates, multi-color well plates, 96-well PCR plates, and tearable 96-well PCR plates, hereinafter referred to as 96-well microplates.

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a method for accurately pasting a sealing film of a 96-well microplate, which is pasted by a jig for pasting a sealing film of a 96-well microplate, the jig comprising a positioning jig seat, the surface of which is provided with positioning jig holes at equal intervals, a sealing film of a plate being provided on one side of the positioning jig seat, a rapid positioning mechanism being provided on the surfaces of the positioning jig seat and the sealing film of the plate being provided, the interior of the rapid positioning mechanism comprising a positioning block, a positioning notch and a positioning convex piece, a material taking mechanism being provided on one side of the positioning jig seat, the material taking mechanism being composed of a material taking component and a receiving cavity; The method for accurately attaching a sealing film to a 96-well microplate comprises the following steps: Placing a 96-well microplate into the holes of the positioning fixture; The sealing film for sealing the plate is arranged on the positioning fixture seat, and the positioning protrusion of the sealing film for sealing the plate is embedded in the positioning notch, so as to facilitate the rapid and accurate positioning of the sealing film for sealing the plate; The plate sealing film is scraped and pressed by a scraper or rolled by a roller, so that the plate sealing film is closely attached to the positioning fixture seat.

[0008] Furthermore, before the step of setting the sealing film on the positioning fixture seat, the method also includes: Pretreatment stage: Before officially starting the film sticking operation, the operator needs to conduct comprehensive and detailed cleaning, inspection and maintenance work on the fixture. Use a dust-free cloth dipped in an appropriate amount of medical alcohol to gently wipe the surface of the positioning fixture seat, the inner wall of the positioning fixture hole, the various parts of the quick positioning mechanism, and the suction cup of the material picking mechanism, and thoroughly remove residual dirt, dust, and possible chemical impurities to ensure that the surface of the fixture is clean and flawless; at the same time, carefully check whether the positioning block is worn, whether the positioning notch is regular, whether the elasticity of the positioning protrusion is normal, and whether the suction cup of the material picking component is damaged or leaking. Once an abnormality is found, replace or repair it immediately; according to the specific specifications and models of the PCR test box to be processed that day, accurately select the appropriate sealing film for the plate, and neatly stack it in the receiving cavity for use, to ensure that the film size and material meet the requirements of the test box.

[0009] Furthermore, the step of placing the 96-well microplate in the positioning fixture hole specifically includes: after washing hands and wearing sterile gloves, the operator holds the PCR test box, aligns its bottom with the positioning fixture hole on the positioning fixture seat, maintains a vertical posture, and slowly and gently places it; during the box placement process, it is necessary to rely on both hand feel and visual confirmation to ensure that the test box falls smoothly into the bottom of the positioning fixture hole, and the surrounding side walls are tightly fitted with the inner wall of the positioning fixture hole without obvious signs of shaking or tilting; the operator places the test boxes in order from left to right and from top to bottom. After filling a row, carefully check the position of each box before processing the next row, so as to ensure the accuracy and efficiency of batch placement.

[0010] Furthermore, the step of using a scraper to scrape or a roller to roll the sealing film so that the sealing film fits tightly on the positioning fixture seat specifically includes: selecting a rubber scraper with a soft texture and moderate elasticity, and placing the scraper at an angle of 30-45 degrees to the surface of the positioning fixture seat, with the starting position set at one end edge of the sealing film. The operator holds the scraper handle with both hands, applies force evenly, and scrapes and presses uniformly from the starting end to the end of the film at a stable and moderate speed. During the scraping and pressing process, the air under the film must be effectively discharged to avoid residual bubbles and prevent damage to the sealing film. After scraping and pressing once, the position can be switched to the other side of the positioning fixture seat and scraped and pressed in the opposite direction again to further enhance the film fitting effect and ensure that the sealing film fits tightly in all directions without dead ends.

[0011] Furthermore, positioning blocks are fixed at the corner positions of the surface of the positioning fixture seat, and positioning gaps are formed between the positioning blocks.

[0012] Furthermore, positioning protrusions are fixed at the center positions of both ends of the sealing film, and the positioning protrusions and the positioning notches are plugged into each other. When the positioning protrusions are plugged into the positioning notches, the sealing film matches the positioning fixture seat.

[0013] Furthermore, a receiving cavity is provided on one side of the positioning fixture seat, the receiving cavity is communicated with the positioning fixture hole, a material picking assembly is provided inside the receiving cavity, and the material picking assembly is located below the positioning fixture hole.

[0014] Furthermore, the interior of the material taking component includes a compression spring, a slide groove and a top plate, the interior of the receiving cavity is provided with a top plate, the inner wall of the receiving cavity is provided with a slide groove, and the slide groove and the top plate are slidably matched with each other.

[0015] Furthermore, a compression spring is fixed to the inner wall of the slide groove, one end of which is fixedly connected to the surface of the top plate. When the compression spring is in a normal state, the bottom of the top plate and the bottom of the positioning fixture seat are located in the same horizontal plane.

[0016] Compared with the prior art, the beneficial effects of the present invention are: the jig for facilitating the sealing film on a 96-well microplate not only improves the working efficiency when the jig is positioned to paste the film, but also improves the convenience when the jig is taken out of the 96-well microplate; By providing a quick positioning mechanism, the sealing film for sealing the plate is placed above the positioning jig seat, and the positioning protrusion is placed inside the positioning notch between the positioning blocks, so that the sealing film for sealing the plate is quickly positioned and placed for subsequent film pasting operations, so as to realize the function of the jig for quickly positioning and placing the sealing film for sealing the plate, thereby improving the working efficiency of the jig when positioning and pasting the film; By providing a material taking mechanism, when the 96-well microplate is inserted into the positioning fixture hole, its bottom contacts the top plate. Since the bottom of the top plate and the bottom of the positioning fixture seat are located at the same horizontal plane in a normal state, the top plate contacts the table top. Since the 96-well microplate is generally a 96-well plate structure, the 96-well plate is inserted into the positioning fixture hole when placed. In order to facilitate film pasting, the 96-well plate is generally in contact with the surface of the positioning fixture seat, and it is not easy to take out the material. After the film pasting is completed, the positioning fixture seat can be lifted. At this time, the top plate can be pushed upward to slide along the slide groove to push out the top of the 96-well microplate, so that the staff can take out the 96-well microplate. After the removal is completed, the top plate will be reset under the elastic force of the compression spring to realize the function of the fixture to facilitate material taking, thereby improving the convenience of the fixture taking out the 96-well microplate. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the three-dimensional appearance structure of the present invention; Figure 2 It is an enlarged structural schematic diagram of the film-applying state of the present invention; Figure 3 It is a schematic diagram of the main structure of the present invention; Figure 4 It is a schematic diagram of an enlarged side cross-section structure of the present invention.

[0018] In the figure: 1. positioning fixture seat; 11. positioning fixture hole; 2. quick positioning mechanism; 21. positioning block; 22. positioning notch; 23. positioning protrusion; 3. sealing film of sealing plate; 4. material taking mechanism; 41. material taking assembly; 411. compression spring; 412. slide groove; 413. top plate; 42. receiving cavity. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. In addition, the terms "first", "second", "third", "upper, lower, left, right", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance. At the same time, in the description of the present invention, unless otherwise clearly specified and limited, the terms "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0020] Reference Figure 1-4A fixture with a 96-well microplate and a sealing film is used, including a positioning fixture seat 1, a surface of which is provided with positioning fixture holes 11 with equal spacing, and a sealing film 3 for sealing a plate is provided on one side of the positioning fixture seat 1.

[0021] Furthermore, if Figure 2 and Figure 3 As shown, a quick positioning mechanism 2 is provided on the surface of the positioning jig seat 1 and the sealing film 3, and the interior of the quick positioning mechanism 2 includes a positioning block 21, a positioning notch 22 and a positioning protrusion 23. Positioning blocks 21 are fixed at the corner positions of the surface of the positioning jig seat 1, and positioning notches 22 are formed between the positioning blocks 21. Positioning protrusions 23 are fixed at the center positions of both ends of the sealing film 3. The positioning protrusions 23 and the positioning notches 22 are plugged into each other. When the positioning protrusions 23 are plugged into the positioning notches 22, the sealing film 3 matches the positioning jig seat 1.

[0022] When in use, place the sealing film 3 on top of the positioning fixture seat 1, so that the positioning protrusion 23 is placed inside the positioning notch 22 between the positioning blocks 21, and quickly position and place the sealing film 3 for subsequent film sticking operations, so as to realize the fixture's function of quickly positioning and placing the sealing film 3.

[0023] Furthermore, if Figure 4 As shown, a material picking mechanism 4 is provided on one side of the positioning fixture seat 1, and the material picking mechanism 4 is composed of a material picking component 41 and a receiving cavity 42. A receiving cavity 42 is provided on one side of the positioning fixture seat 1, and the receiving cavity 42 is communicated with the positioning fixture hole 11. A material picking component 41 is provided inside the receiving cavity 42, and the material picking component 41 is located below the positioning fixture hole 11. The interior of the material picking component 41 includes a compression spring 411, a slide groove 412 and a top plate 413. The interior of the receiving cavity 42 is provided with a top plate 413, and the inner wall of the receiving cavity 42 is provided with a slide groove 412, and the slide groove 412 and the top plate 413 slide with each other. A compression spring 411 is fixed to the inner wall of the slide groove 412, and one end of the compression spring 411 is fixedly connected to the surface of the top plate 413. When the compression spring 411 is in a normal state, the bottom of the top plate 413 and the bottom of the positioning fixture seat 1 are located in the same horizontal plane.

[0024] When in use, when the 96-well microplate is inserted into the positioning fixture hole 11, its bottom contacts the top plate 413. Since the bottom of the top plate 413 and the bottom of the positioning fixture seat 1 are located at the same horizontal plane in the normal state, the top plate 413 contacts the table top. Since the 96-well microplate is generally a 96-well plate structure, the 96-well plate is inserted into the positioning fixture hole 11 when placed. In order to facilitate film pasting, the 96-well plate is generally in contact with the surface of the positioning fixture seat 1, which is not easy to remove materials. After the film pasting is completed, the positioning fixture seat 1 can be lifted. At this time, the top plate 413 can be pushed upward to slide the top plate 413 along the slide groove 412 to push out the top of the 96-well microplate so that the staff can take out the 96-well microplate. After the removal is completed, the top plate 413 will be reset under the elastic force of the compression spring 411 to realize the function of the fixture to facilitate material removal.

[0025] Working principle: When in use, first place the jig on the table, then insert the 96-well microplate to be pasted into the positioning jig hole 11, then place the sealing film 3 on the top of the positioning jig seat 1, so that the positioning protrusion 23 is placed in the positioning notch 22 between the positioning blocks 21, and the sealing film 3 is quickly positioned and placed for subsequent film pasting operations, so as to realize the jig's function of quickly positioning and placing the sealing film 3, thereby improving the work efficiency of the jig when positioning the film.

[0026] When the 96-well microplate is inserted into the positioning fixture hole 11, its bottom is in contact with the top plate 413. Since the bottom of the top plate 413 and the bottom of the positioning fixture seat 1 are located at the same horizontal plane in the normal state, the top plate 413 is in contact with the table surface. Since the 96-well microplate is generally a 96-well plate structure, the 96-well plate is inserted into the positioning fixture hole 11 when placed. In order to facilitate film pasting, the 96-well plate is generally in contact with the surface of the positioning fixture seat 1, which is not easy to remove materials. After the film pasting is completed, the positioning fixture seat 1 can be lifted. At this time, the top plate 413 can be pushed upward to make the top plate 413 slide along the slide groove 412 to push out the top of the 96-well microplate so that the staff can take out the 96-well microplate. After the removal is completed, the top plate 413 will be reset under the elastic force of the compression spring 411 to realize the function of the fixture to facilitate material removal, thereby improving the convenience of the fixture in taking out the 96-well microplate, and finally completing the use of the fixture.

[0027] The present invention provides a method for accurately pasting a sealing film of a 96-well microplate, which is pasted using a jig for pasting a sealing film of a 96-well microplate. The method for accurately pasting a sealing film of a 96-well microplate comprises the following steps: Placing a 96-well microplate into the holes of the positioning fixture; The sealing film for sealing the plate is arranged on the positioning fixture seat, and the positioning protrusion of the sealing film for sealing the plate is embedded in the positioning notch, so as to facilitate the rapid and accurate positioning of the sealing film for sealing the plate; The plate sealing film is scraped and pressed by a scraper or rolled by a roller, so that the plate sealing film is closely attached to the positioning fixture seat.

[0028] Furthermore, before the step of setting the sealing film on the positioning fixture seat, the method also includes: Pretreatment stage: Before officially starting the film sticking operation, the operator needs to conduct comprehensive and detailed cleaning, inspection and maintenance work on the fixture. Use a dust-free cloth dipped in an appropriate amount of medical alcohol to gently wipe the surface of the positioning fixture seat, the inner wall of the positioning fixture hole, the various parts of the quick positioning mechanism, and the suction cup of the material picking mechanism, and thoroughly remove residual dirt, dust, and possible chemical impurities to ensure that the surface of the fixture is clean and flawless; at the same time, carefully check whether the positioning block is worn, whether the positioning notch is regular, whether the elasticity of the positioning protrusion is normal, and whether the suction cup of the material picking component is damaged or leaking. Once an abnormality is found, replace or repair it immediately; according to the specific specifications and models of the PCR test box to be processed that day, accurately select the appropriate sealing film for the plate, and neatly stack it in the receiving cavity for use, to ensure that the film size and material meet the requirements of the test box.

[0029] Furthermore, the step of placing the 96-well microplate in the positioning fixture hole specifically includes: after washing hands and wearing sterile gloves, the operator holds the PCR test box, aligns its bottom with the positioning fixture hole on the positioning fixture seat, maintains a vertical posture, and slowly and gently places it; during the box placement process, it is necessary to rely on both hand feel and visual confirmation to ensure that the test box falls smoothly into the bottom of the positioning fixture hole, and the surrounding side walls are tightly fitted with the inner wall of the positioning fixture hole without obvious signs of shaking or tilting; the operator places the test boxes in order from left to right and from top to bottom. After filling a row, carefully check the position of each box before processing the next row, so as to ensure the accuracy and efficiency of batch placement.

[0030] Furthermore, the step of using a scraper to scrape or a roller to roll the sealing film so that the sealing film fits tightly on the positioning fixture seat specifically includes: selecting a rubber scraper with a soft texture and moderate elasticity, and placing the scraper at an angle of 30-45 degrees to the surface of the positioning fixture seat, with the starting position set at one end edge of the sealing film. The operator holds the scraper handle with both hands, applies force evenly, and scrapes and presses uniformly from the starting end to the end of the film at a stable and moderate speed. During the scraping and pressing process, the air under the film must be effectively discharged to avoid residual bubbles and prevent damage to the sealing film. After scraping and pressing once, the position can be switched to the other side of the positioning fixture seat and scraped and pressed in the opposite direction again to further enhance the film fitting effect and ensure that the sealing film fits tightly in all directions without dead ends.

[0031] By providing a quick positioning mechanism, the sealing film for sealing the plate is placed above the positioning jig seat, and the positioning protrusion is placed inside the positioning notch between the positioning blocks, so that the sealing film for sealing the plate is quickly positioned and placed for subsequent film pasting operations, so as to realize the function of the jig for quickly positioning and placing the sealing film for sealing the plate, thereby improving the working efficiency of the jig when positioning and pasting the film; By providing a material taking mechanism, when the 96-well microplate is inserted into the positioning fixture hole, its bottom contacts the top plate. Since the bottom of the top plate and the bottom of the positioning fixture seat are located at the same horizontal plane in a normal state, the top plate contacts the table top. Since the 96-well microplate is generally a 96-well plate structure, the 96-well plate is inserted into the positioning fixture hole when placed. In order to facilitate film pasting, the 96-well plate is generally in contact with the surface of the positioning fixture seat, and it is not easy to take out the material. After the film pasting is completed, the positioning fixture seat can be lifted. At this time, the top plate can be pushed upward to slide along the slide groove to push out the top of the 96-well microplate, so that the staff can take out the 96-well microplate. After the removal is completed, the top plate will be reset under the elastic force of the compression spring to realize the function of the fixture to facilitate material taking, thereby improving the convenience of the fixture taking out the 96-well microplate.

[0032] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A method for accurately covering a sealing film of a 96-well microplate, characterized in that: A 96-well microplate sealing film fixture is used for pasting, the fixture comprising a positioning fixture seat (1), the surface of the positioning fixture seat (1) is provided with positioning fixture holes (11) at equal intervals, one side of the positioning fixture seat (1) is provided with a sealing film (3) for sealing the plate, a rapid positioning mechanism (2) is provided on the surfaces of the positioning fixture seat (1) and the sealing film (3), the interior of the rapid positioning mechanism (2) comprises a positioning block (21), a positioning notch (22) and a positioning protrusion (23), one side of the positioning fixture seat (1) is provided with a material taking mechanism (4), the material taking mechanism (4) is composed of a material taking component (41) and a receiving cavity (42); The method for accurately attaching a sealing film to a 96-well microplate comprises the following steps: Placing a 96-well microplate into the positioning fixture hole (11); The sealing film (3) is arranged on the positioning fixture seat (1), and the positioning protrusion (23) of the sealing film (3) is embedded in the positioning notch (22), so as to facilitate the rapid and accurate positioning of the sealing film (3); The plate sealing film (3) is scraped and pressed by a scraper or rolled and pressed by a roller, so that the plate sealing film (3) is tightly attached to the positioning fixture seat (1).

2. The method for accurately covering a sealing film of a 96-well microplate according to claim 1, characterized in that: Before the step of setting the sealing film (3) on the positioning fixture seat (1), the method also includes: pre-treatment step: before officially starting the film sticking operation, the operator needs to perform comprehensive and detailed cleaning and inspection and maintenance work on the fixture. Use a dust-free cloth dipped in an appropriate amount of medical alcohol to gently wipe the surface of the positioning fixture seat, the inner wall of the positioning fixture hole, the various components of the quick positioning mechanism, and the suction cup of the material picking mechanism, and thoroughly remove residual dirt, dust, and possible chemical impurities to ensure that the surface of the fixture is clean and flawless; at the same time, carefully check whether the positioning block is worn, whether the positioning notch is regular, whether the positioning convex piece is elastic, and whether the suction cup of the material picking component is damaged or leaking. Once an abnormality is found, replace or repair it immediately; according to the specific specifications and models of the PCR test box to be processed that day, accurately select the appropriate sealing film for the plate, and neatly stack it in the storage cavity for use, to ensure that the film size and material meet the requirements of the test box.

3. The method for accurately covering a sealing film of a 96-well microplate according to claim 1, characterized in that: The step of placing the 96-well microplate in the positioning fixture hole (11) specifically includes: after washing hands and wearing sterile gloves, the operator holds the PCR test box, aligns its bottom with the positioning fixture hole on the positioning fixture seat, keeps it in a vertical position, and slowly and gently places it; during the box placement process, it is necessary to double-check by hand and vision to ensure that the test box falls smoothly into the bottom of the positioning fixture hole, and that the surrounding side walls are tightly fitted with the inner wall of the positioning fixture hole without obvious shaking or tilting; the operator places the test boxes in order from left to right and from top to bottom. After a row is filled, the operator carefully checks the position of each box and then proceeds to the next row, so as to ensure the accuracy and efficiency of batch placement.

4. The method for accurately covering a sealing film of a 96-well microplate according to claim 1, characterized in that: The step of using a scraper to scrape or a roller to press the sealing film (3) so that the sealing film (3) is tightly attached to the positioning fixture seat (1) specifically includes: selecting a rubber scraper with a soft texture and moderate elasticity, and placing the scraper at an angle of 30-45 degrees to the surface of the positioning fixture seat, with the starting position set at one end edge of the sealing film. The operator holds the scraper handle with both hands, applies force evenly, and scrapes and presses at a stable and moderate speed from the starting end of the film to the end. During the scraping and pressing process, the air under the film must be effectively discharged to avoid residual bubbles and prevent damage to the sealing film. After scraping and pressing once, the position can be switched to the other side of the positioning fixture seat and scraped and pressed in the opposite direction again to further enhance the film adhesion effect and ensure that the sealing film is tightly attached in all directions without dead corners.

5. The method for accurately covering a sealing film of a 96-well microplate according to claim 1, characterized in that: Positioning blocks (21) are fixed at the corner positions of the surface of the positioning fixture seat (1), and positioning notches (22) are formed between the positioning blocks (21).

6. The method for accurately attaching a sealing film to a 96-well microplate according to claim 5, characterized in that: Positioning protrusions (23) are fixed at the center positions of both ends of the sealing plate sealing film (3), and the positioning protrusions (23) and the positioning notches (22) are plugged into each other. When the positioning protrusions (23) and the positioning notches (22) are plugged into each other, the sealing plate sealing film (3) matches the positioning fixture seat (1).

7. The method for accurately attaching a sealing film to a 96-well microplate according to claim 6, characterized in that: A receiving cavity (42) is provided on one side of the positioning fixture seat (1), the receiving cavity (42) is communicated with the positioning fixture hole (11), a material taking component (41) is provided inside the receiving cavity (42), and the material taking component (41) is located below the positioning fixture hole (11).

8. The method for accurately covering a sealing film of a 96-well microplate according to claim 7, characterized in that: The interior of the material taking component (41) includes a compression spring (411), a slide groove (412) and a top plate (413); the interior of the receiving cavity (42) is provided with a top plate (413); the inner wall of the receiving cavity (42) is provided with a slide groove (412); the slide groove (412) and the top plate (413) are slidably matched with each other.

9. The method for accurately attaching a sealing film to a 96-well microplate according to claim 8, characterized in that: A compression spring (411) is fixed to the inner wall of the slide groove (412), one end of the compression spring (411) is fixedly connected to the surface of the top plate (413), and when the compression spring (411) is in a normal state, the bottom of the top plate (413) and the bottom of the positioning fixture seat (1) are located in the same horizontal plane.

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