Inoculation device for efficiently inoculating microbial bacterial liquid in large-size container

Abstract

The invention discloses an inoculation device for efficiently inoculating a microbial liquid in a large-size container, and relates to the technical field of efficiently inoculating the microbial liquid in the large-size container. The fixing and rotating assembly is arranged on a workbench and used for fixing and driving the large-size container to rotate around the axis of the large-size container, the servo motor is fixedly arranged on the bottom surface of the workbench, an output shaft of the servo motor upwards penetrates through the workbench, a main shaft is connected to the extending end of the servo motor, and a strip-shaped plate is fixedly arranged at the top of the main shaft; the left end of the strip-shaped plate is provided with an automatic cover dismounting assembly used for dismounting a plastic cover of the large-size container, the automatic cover dismounting assembly is located over the fixing and rotating assembly, and the right end of the strip-shaped plate is provided with an automatic dispersing assembly used for dispersing microorganism bacterium liquid in a container body of the large-size container in a flower shape. The device has the beneficial effects that the plastic cover is tightly covered, and the microbial liquid inoculation efficiency is greatly improved.

Description

Technical Field

[0001] The invention relates to the technical field of efficiently inoculating microbial liquid in a large-sized container, in particular to an inoculation device for efficiently inoculating microbial liquid in a large-sized container. Background Art

[0002] Microbial inoculation is to disperse a certain amount of microbial liquid in the culture medium in the culture dish, and obtain a large number of independently distributed microbial colonies after cultivation. Since the microbial colonies are visible to the naked eye, it is convenient for workers to extract valuable components from the microbial colonies. Since the diameter of the culture dish is generally less than 15cm, it is impossible to inoculate a large amount of microbial liquid. Therefore, the staff uses Figure 1~Figure 4 The large-sized container shown is used to inoculate microbial liquid. The large-sized container includes a container body 1 and a plastic cover 2 covering the top end of the container body 1. The bottom of the container body 1 is closed, and the diameter of the container body 1 is 25 cm.

[0003] The method by which the staff inoculates the microbial liquid into the large-sized container is as follows: S1. A staff member takes out a large-sized container and supports the container body 1 of the large-sized container on a table; then the staff member removes the plastic cover 2 from the container body 1; S2. The staff adds a certain amount of culture solution into the container body 1; S3, the staff takes out an inoculator, uses the inoculator to extract a certain amount of microbial liquid from the storage tank; then the liquid outlet port of the inoculator is brought into contact with the liquid surface of the culture liquid; the staff squeezes the air bag of the inoculator, and the microbial liquid in the inoculator flows out from the liquid outlet port of the inoculator, and then the staff moves the inoculator in a flower shape. When the inoculator is moved to the initial position, the first batch of microbial liquid can be dispersed in the culture liquid of the container body 1 in a flower shape; S4, the staff repeats the operation of step S3 for multiple times, so that multiple batches of microbial liquid can be dispersed in the culture liquid of the container body 1 in a flower shape; S5. The staff member re-closes the plastic cover 2 on the top port of the container body 1 to seal the culture solution and the microbial liquid, thereby finally achieving the inoculation of the microbial liquid in the large-sized container; S6. The staff repeats the operations of steps S1 to S5 for multiple times, and then multiple large-sized containers can be inoculated with microbial liquid.

[0004] However, although this method can inoculate microbial liquid in large-sized containers, it still reflects the following technical defects in actual operation: I. In step S1, when the staff pulls the bottom surface of the side of the plastic cover 2 upward with their left and right hands respectively, the pulling direction is as follows: Figure 2 As shown by the solid arrow in the middle, since the top of the plastic cover 2 is still tightly attached to the top surface of the container body 1, the top of the plastic cover 2 blocks the upward movement of the side of the plastic cover 2, thereby causing the side of the plastic cover 2 to deform outward, which results in that in step S5, after the staff re-closes the plastic cover 2 on the top port of the container body 1, the plastic cover 2 cannot seal the top port of the container body 1, that is, there is a gap between the side of the plastic cover 2 and the cylindrical surface of the container body 1. Therefore, there is a technical defect that the plastic cover 2 is not tightly closed.

[0005] In addition, it is not only necessary to manually remove the plastic cover 2 from the container body 1, but also necessary to manually re-cover the plastic cover 2 on the container body 1; and the entire operation is completed manually, thereby extending the inoculation time of the microbial liquid and reducing the inoculation efficiency of the microbial liquid.

[0006] II. In steps S3-S4, the staff is required to first extract a certain amount of microbial liquid from the storage tank using an inoculator, and then manually move the inoculator in a flower shape to disperse the microbial liquid in the culture liquid of the container body 1 in a flower shape; and the entire operation is completed manually, and there are many operating procedures, which undoubtedly takes a long time to disperse multiple batches of microbial liquid in the culture liquid of the container body 1 in a flower shape, thereby further reducing the inoculation efficiency of the microbial liquid.

[0007] Therefore, there is an urgent need for an inoculation device that has a tight plastic cover and greatly improves the efficiency of microbial liquid inoculation. Summary of the invention

[0008] The purpose of the present invention is to overcome the shortcomings of the prior art and provide an inoculation device for efficiently inoculating microbial liquid in a large-sized container, which has a tightly closed plastic cover and greatly improves the inoculation efficiency of the microbial liquid.

[0009] The object of the present invention is achieved through the following technical solutions: an inoculation device for efficiently inoculating microbial liquid in a large-sized container, which includes a fixing and rotating assembly arranged on a workbench for fixing and driving the large-sized container to rotate around its own axis, and a servo motor fixedly arranged on the bottom surface of the workbench, the output shaft of the servo motor passes through the workbench upward, and a main shaft is connected to the extended end, a strip plate is fixedly arranged on the top of the main shaft, an automatic cover removal assembly for removing the plastic cover of the large-sized container is arranged on the left end of the strip plate, and the automatic cover removal assembly is located directly above the fixing and rotating assembly, and an automatic dispersing assembly for dispersing the microbial liquid in a flower shape in the container body of the large-sized container is arranged on the right end of the strip plate; The automatic dispersion component includes a lifting cylinder fixedly mounted on the right end of the strip plate, a piston rod of the lifting cylinder passes through the strip plate downward, and a lifting plate is fixedly mounted on the extended end, an L plate is fixedly mounted on the bottom surface of the left end of the lifting plate, an annular ring is fixedly mounted on the end portion of the L plate, a plurality of arc grooves arranged at intervals are opened on the inner wall of the center hole of the annular ring along its circumference, a driving motor is fixedly mounted on the bottom surface of the right end of the lifting plate, and a connecting plate passing through the center hole of the annular ring downward is fixedly mounted on the output shaft of the driving motor; A movable bar extending to the right is slidably penetrated in the lower end of the connecting plate, and a fixed plate, an inoculation tube and a rod are fixedly arranged on the top surface of the movable bar in sequence from left to right, and a spring is fixedly arranged between the fixed plate and the connecting plate; the top end of the inoculation tube passes through the central hole of the annular ring upward and extends above the annular ring, and a nylon wheel is rotatably installed on the top end of the rod, and under the elastic force of the spring, the nylon wheel abuts against the bottom of the rightmost arc-shaped groove in the annular ring; A storage tank for storing microbial liquid is fixedly provided on the left end surface of the connecting plate, a peristaltic pump is fixedly provided on the bottom surface of the connecting plate, a liquid suction pipe is connected to the liquid suction port of the peristaltic pump, the liquid suction pipe passes through the connecting plate and is connected to the bottom port of the storage tank, a liquid discharge port of the peristaltic pump is connected to a liquid discharge pipe, and the liquid discharge pipe is connected to the top port of the inoculation tube.

[0010] The axis of the driving motor is coaxial with the axis of the annular ring.

[0011] The arc-shaped grooves are evenly distributed on the inner wall of the annular ring.

[0012] A support plate is fixedly arranged on the left end surface of the connecting plate, and the storage tank is fixedly arranged on the support plate.

[0013] The fixed and rotating components include a stepper motor fixed on the workbench surface and an arch frame A fixed on the output shaft of the stepper motor. A turntable is fixed on the top of the arch frame A. A positioning hole is opened on the top surface of the turntable. The diameter of the positioning hole is equal to the outer diameter of the container body of the large-size container. A step hole penetrating the bottom surface of the turntable is opened at the bottom of the positioning hole. A suction cup A is fixed in the large hole of the step hole. The top surface of the suction cup A is flush with the bottom of the positioning hole. A plurality of vacuum holes A connected to the inner cavity of the suction cup A are opened on the top surface. A vacuum pump A is fixed in the arch frame A. The working port of the vacuum pump A is connected to the inner cavity of the suction cup A through a pipeline.

[0014] The automatic cover removal assembly includes a cover removal cylinder fixedly arranged at the left end of the strip plate, the piston rod of the cover removal cylinder penetrates the strip plate downward, and an arch frame B is fixedly arranged on the extended end, a suction cup B is fixedly arranged on the bottom surface of the arch frame B, a plurality of vacuum holes B connected to the inner cavity of the suction cup B are opened on the bottom surface of the suction cup B, a vacuum pump B is fixedly arranged in the arch frame B, and a working port of the vacuum pump B is connected to the inner cavity of the suction cup B; Horizontal cylinders are fixedly provided on the top surface of the suction cup B and on its left and right sides, the piston rods of the two horizontal cylinders extend outside the suction cup B, and a vertical plate is fixedly provided on the extended end, longitudinally arranged lifting plates are fixedly provided on the inner end surfaces of the two vertical plates, and semicircular holes are opened on the inner end surfaces of the two lifting plates, and the diameter of the semicircular holes is equal to the outer diameter of the container body of the large-size container.

[0015] A plurality of legs supported on the ground are fixedly arranged on the bottom surface of the workbench.

[0016] The inoculation device also includes a controller, which is electrically connected to a servo motor, a drive motor, a stepper motor, a lifting cylinder, a cover removal cylinder, a horizontal cylinder, a peristaltic pump, a vacuum pump A, and a vacuum pump B via a signal line.

[0017] The invention has the following advantages: the plastic cover is tightly closed and the inoculation efficiency of the microbial liquid is greatly improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the structure of a large-sized container; Figure 2 for Figure 1 The main cross-sectional diagram of Figure 3 A schematic diagram of the structure of a container body of a large-sized container; Figure 4 A schematic diagram of the structure of a plastic cover for a large-sized container; Figure 5 It is a schematic diagram of dispersing the first batch of microbial liquid in the culture liquid of the container body in a flower shape; Figure 6 It is a schematic diagram of dispersing multiple batches of microbial liquid in the culture liquid of the container body in a flower shape; Figure 7 It is a structural schematic diagram of the present invention; Figure 8 for Figure 7 The main cross-sectional diagram of Fig. 9 It is a schematic diagram of the structure of the fixed and rotating components; Fig.10 for Fig. 9 Bottom view of Fig.11 for Fig.10 The main cross-sectional diagram of Fig.12 It is a structural schematic diagram of the automatic cover removal assembly; Fig.13 for Fig.11 The main cross-sectional diagram of Fig.14 It is a schematic diagram of the structure of the automatic dispersion component; Fig.15 for Fig.14 Bottom view of Fig.16 for Fig.14 The main cross-sectional diagram of Fig.17 It is a schematic diagram of the annular ring structure; Fig.18 It is a schematic diagram of the connection between the connecting plate, the movable bar, the nylon wheel, the spring and the inoculation tube; Fig.19 A schematic diagram of inserting a container body of a large-sized container into a positioning hole of a fixed and rotating assembly; Fig. 20 is a schematic diagram showing that the bottom surface of the suction cup B is in contact with the top of the plastic cover; Fig.21 for Fig. 20 A partial enlarged view of the M part; Fig. 22 It is a schematic diagram of the semicircular holes of the two lifting plates being respectively clamped on the outer cylindrical surface of the container body; Fig.23 for Fig. 22 A partial enlarged view of part N; Fig.24 A schematic diagram of automatically removing a plastic cover from a container body; Fig.25 A schematic diagram of the inoculation tube of the automatic dispersing assembly moving to the top of the container body; Fig.26 It is a schematic diagram showing that the bottom port of the inoculation tube is in contact with the liquid surface of the culture solution in the container body; Fig. 27 for Fig.26 A partial enlarged view of the P part; Fig.28 It is a schematic diagram of the turntable driving the container body to rotate synchronously; Fig.29 A schematic diagram of the plastic cover being re-closed on the container body; Fig.30 A schematic diagram of a worker taking away a large container inoculated with microbial liquid; In the figure: 1-Container body, 2-Plastic cover; 3- working table, 4- fixed and rotating components, 5- servo motor, 6- spindle, 7- strip plate, 8- automatic cover removal component, 9- automatic dispersion component; 10-lifting cylinder, 11-lifting plate, 12-L plate, 13-annular ring, 14-arc groove, 15-driving motor, 16-connecting plate, 17-movable bar, 18-fixed plate, 19-inoculation tube, 20-rod, 21-spring, 22-nylon wheel, 23-storage tank, 24-peristaltic pump, 25-liquid extraction tube, 26-liquid discharge tube; 27-stepping motor, 28-turntable, 29-positioning hole, 30-suction cup A, 31-vacuum hole A, 32-vacuum pump A; 33-cover removal cylinder, 34-suction cup B, 35-vacuum hole B, 36-vacuum pump B, 37-horizontal cylinder, 38-lifting plate, 39-semicircular hole. DETAILED DESCRIPTION

[0019] The present invention is further described below in conjunction with the accompanying drawings, and the protection scope of the present invention is not limited to the following: like Figure 7 to Figure 18 As shown, an inoculation device for efficiently inoculating microbial liquid in a large-sized container comprises a fixing and rotating assembly 4 arranged on a workbench 3 for fixing and driving the large-sized container to rotate around its own axis, a servo motor 5 fixedly arranged on the bottom surface of the workbench 3, the output shaft of the servo motor 5 upwardly passes through the workbench 3, and a main shaft 6 is connected to the extended end, a strip plate 7 is fixedly arranged on the top of the main shaft 6, an automatic cover removal assembly 8 for removing the plastic cover 2 of the large-sized container is arranged on the left end of the strip plate 7, and the automatic cover removal assembly 8 is located directly above the fixing and rotating assembly 4, and an automatic dispersion assembly 9 for dispersing the microbial liquid in a flower shape in the container body 1 of the large-sized container is arranged on the right end of the strip plate 7; a plurality of legs supported on the ground are fixedly arranged on the bottom surface of the workbench 3.

[0020] The automatic dispersion component 9 includes a lifting cylinder 10 fixedly arranged on the right end of the strip plate 7, the piston rod of the lifting cylinder 10 penetrates the strip plate 7 downward, and a lifting plate 11 is fixedly arranged on the extended end, an L plate 12 is fixedly arranged on the bottom surface of the left end of the lifting plate 11, an annular ring 13 is fixedly arranged on the terminal end of the L plate 12, a plurality of arc grooves 14 arranged at intervals are opened on the inner wall of the center hole of the annular ring 13 along its circumference, and the arc grooves 14 are evenly distributed on the inner wall of the annular ring 13, a driving motor 15 is fixedly arranged on the bottom surface of the right end of the lifting plate 11, and a connecting plate 16 penetrating downward through the center hole of the annular ring 13 is fixedly arranged on the output shaft of the driving motor 15; the axis of the driving motor 15 is coaxial with the axis of the annular ring 13.

[0021] A movable bar 17 extending to the right is slidably penetrated in the lower end of the connecting plate 16, and a fixed plate 18, an inoculation tube 19 and a rod 20 are fixed on the top surface of the movable bar 17 from left to right in sequence, and a spring 21 is fixed between the fixed plate 18 and the connecting plate 16; the top end of the inoculation tube 19 penetrates the central hole of the annular ring 13 upward and extends above the annular ring 13, and a nylon wheel 22 is rotatably installed on the top of the rod 20, and under the elastic force of the spring 21, the nylon wheel 22 2 abuts against the bottom of the rightmost arc groove 14 in the annular ring 13; a storage tank 23 storing microbial liquid is fixedly arranged on the left end surface of the connecting plate 16, a peristaltic pump 24 is fixedly arranged on the bottom surface of the connecting plate 16, a liquid extraction pipe 25 is connected to the liquid extraction port of the peristaltic pump 24, the liquid extraction pipe 25 passes through the connecting plate 16 and is connected to the bottom port of the storage tank 23, a liquid discharge pipe 26 is connected to the liquid discharge port of the peristaltic pump 24, and the liquid discharge pipe 26 is connected to the top port of the inoculation tube 19. A support plate is fixedly arranged on the left end surface of the connecting plate 16, and the storage tank 23 is fixedly arranged on the support plate.

[0022] The fixed and rotating component 4 includes a stepper motor 27 fixedly mounted on the surface of the workbench 3, and an arch frame A fixedly mounted on the output shaft of the stepper motor 27. A turntable 28 is fixedly mounted on the top of the arch frame A. A positioning hole 29 is provided on the top surface of the turntable 28. The diameter of the positioning hole 29 is equal to the outer diameter of the container body 1 of the large-size container. A step hole penetrating the bottom surface of the turntable 28 is provided at the bottom of the positioning hole 29. A suction cup A30 is fixedly mounted in the large hole of the step hole. The top surface of the suction cup A30 is flush with the bottom of the positioning hole 29. A plurality of vacuum holes A31 connected to the inner cavity thereof are provided on the top surface of the suction cup A30. A vacuum pump A32 is fixedly mounted in the arch frame A. The working port of the vacuum pump A32 is connected to the inner cavity of the suction cup A30 via a pipeline.

[0023] The automatic lid removal assembly 8 includes a lid removal cylinder 33 fixedly arranged at the left end of the strip plate 7, the piston rod of the lid removal cylinder 33 penetrates the strip plate 7 downward, and an arch frame B is fixedly arranged on the extended end, a suction cup B34 is fixedly arranged on the bottom surface of the arch frame B, a plurality of vacuum holes B35 connected to the inner cavity thereof are provided on the bottom surface of the suction cup B34, a vacuum pump B36 is fixedly arranged in the arch frame B, and the working port of the vacuum pump B36 is connected to the inner cavity of the suction cup B34; horizontal cylinders 37 are fixedly arranged on the top surface of the suction cup B34 and on its left and right sides, the piston rods of the two horizontal cylinders 37 are extended to the outside of the suction cup B34, and a vertical plate is fixedly arranged on the extended end, longitudinally arranged lifting plates 38 are fixedly arranged on the inner end surfaces of the two vertical plates, semicircular holes 39 are provided on the inner end surfaces of the two lifting plates 38, and the diameter of the semicircular holes 39 is equal to the outer diameter of the container body 1 of the large-size container.

[0024] The inoculation device also includes a controller, which is electrically connected to the servo motor 5, the drive motor 15, the stepper motor 27, the lifting cylinder 10, the cover removal cylinder 33, the horizontal cylinder 37, the peristaltic pump 24, the vacuum pump A32, and the vacuum pump B36 via a signal line. The staff can control the start or stop of the servo motor 5, the drive motor 15, the stepper motor 27, the peristaltic pump 24, the vacuum pump A32, and the vacuum pump B36 through the controller. At the same time, the extension or retraction of the piston rods of the lifting cylinder 10, the cover removal cylinder 33, and the horizontal cylinder 37 can also be controlled, thereby facilitating the operation of the staff and having the characteristics of a high degree of automation.

[0025] The working steps of the present invention are: S1. The staff takes out a Figure 1~Figure 2 The large-sized container shown in the figure is inserted from top to bottom into the positioning hole 29 of the turntable 28 of the fixed and rotating assembly 4, as shown in FIG. Fig.19 As shown, at this time, the large-sized container enters the decapping station of the automatic decapping assembly 8, and the bottom surface of the container body 1 of the large-sized container completely covers the vacuum hole A31 of the suction cup A30. At the same time, the plastic cover 2 of the large-sized container is located directly above the positioning hole 29, and the plastic cover 2 is just directly above the suction cup B34 of the automatic decapping assembly 8. S2, removing the plastic cover 2 from the container body 1, the specific operation steps are: S21, control the vacuum pump A32 of the fixed and rotating assembly 4 to start, and the vacuum pump A32 evacuates the inner cavity of the suction cup A30 and each vacuum hole A31, and under negative pressure, the container body 1 of the large-sized container is adsorbed and fixed on the suction cup A30; S22, control the piston rod of the cover removal cylinder 33 of the automatic cover removal assembly 8 to extend downward, the piston rod drives the arch frame B to move downward, and the arch frame B drives the vacuum pump B36, the suction cup B34, the two horizontal cylinders 37 and the lifting plate 38 to move downward synchronously; when the piston rod of the cover removal cylinder 33 is fully extended, the bottom surface of the suction cup B34 just contacts with the top of the plastic cover 2, as shown in FIG. Figure 20~Figure 21 As shown, at the same time, the semicircular holes 39 of the two lifting plates 38 are respectively located on the left and right sides of the container body 1 of the large-sized container; S23, control the piston rods of the two horizontal cylinders 37 to retract, the piston rods drive the vertical plate to move toward the container body 1, and the vertical plate drives the semicircular holes 39 of the lifting plate 38 to move toward the container body 1 synchronously. When the piston rods of the two horizontal cylinders 37 are fully retracted, the semicircular holes 39 of the two lifting plates 38 are respectively stuck on the outer cylindrical surface of the container body 1, as shown in FIG. Figure 22~Figure 23 As shown, at the same time, the top surfaces of the two lifting plates 38 are in contact with the bottom surface of the side of the plastic cover 2; S24, control the vacuum pump B36 to start, the vacuum pump B36 evacuates the inner cavity of the suction cup B34 and each vacuum hole B35, and under negative pressure, the plastic cover 2 of the large-sized container is adsorbed and fixed on the suction cup B34; S25, control the piston rod of the cover removal cylinder 33 of the automatic cover removal assembly 8 to retract upward, the piston rod drives the arch frame B to move upward, and the arch frame B drives the vacuum pump B36, the suction cup B34, the two horizontal cylinders 37 and the lifting plate 38 to move upward synchronously, wherein the suction cup B34 drives the top of the plastic cover 2 to move upward, and at the same time, the two lifting plates 38 drive the side of the plastic cover 2 to move upward, thereby gradually removing the plastic cover 2 from the container body 1; when the piston rod of the cover removal cylinder 33 is fully retracted, the plastic cover 2 can be automatically removed from the container body 1, as shown in FIG. Fig.24 As shown; Among them, it can be known in step S25 that while the suction cup B34 drives the top of the plastic cover 2 to move upward, the two lifting plates 38 also lift the sides of the plastic cover 2 upward, so that the top and sides of the plastic cover 2 move upward synchronously. Therefore, compared with the manual cover removal method, the inoculation device effectively avoids the deformation of the side of the plastic cover 2 caused by the top of the plastic cover 2 blocking the upward movement of the side of the plastic cover 2 when the staff pulls the bottom surface of the side of the plastic cover 2 upward, thereby ensuring that in S82, after the plastic cover 2 is re-covered on the top port of the container body 1, the plastic cover 2 can tightly seal the top port of the container body 1, and has the technical characteristics of tight covering.

[0026] In addition, the inoculation device can automatically remove the plastic cover 2 from the container body 1 or automatically cover the plastic cover 2 on the container body 1 through the linkage cooperation of the fixing and rotating component 4 and the automatic cover removal component 8. There is no need to manually remove the plastic cover 2 from the container body 1 or manually re-cover the plastic cover 2 on the container body 1, thereby shortening the inoculation time of the microbial solution and greatly improving the inoculation efficiency of the microbial solution.

[0027] S3, after removing the plastic cover 2 on the container body 1, the staff adds a certain amount of culture solution into the container body 1; S4, control the servo motor 5 to start, the servo motor 5 drives the main shaft 6 to rotate, the main shaft 6 drives the strip plate 7 to rotate, the strip plate 7 drives the automatic cover removal component 8 and the automatic dispersion component 9 to rotate synchronously, the automatic cover removal component 8 drives the removed plastic cover 2 to rotate synchronously, when the strip plate 7 rotates 180°, the controller controls the servo motor 5 to turn off, at this time, the inoculation tube 19 of the automatic dispersion component 9 just moves to the top of the container body 1, as shown in FIG. Fig.25 As shown; S5, dispersing the first batch of microbial liquid in the culture liquid of the container body 1 in a flower shape, and the specific operation steps are as follows: S51, control the piston rod of the lifting cylinder 10 of the automatic dispersion component 9 to extend downward, the piston rod drives the lifting plate 11 to move downward, and the lifting plate 11 drives the various components connected thereto to move downward synchronously. When the piston rod of the lifting cylinder 10 is fully extended, the bottom end of the inoculation tube 19 just contacts the liquid surface of the culture solution in the container body 1, as shown in FIG. Figure 26-27 As shown; S52, the peristaltic pump 24 of the automatic dispersing component 9 is controlled to start, and the peristaltic pump 24 extracts the microbial liquid in the storage tank 23. Under the pump pressure, the microbial liquid passes through the liquid extraction pipe 25, the peristaltic pump 24, the liquid discharge pipe 26, the top port of the inoculation tube 19, the inoculation tube 19, and finally is discharged from the bottom port of the inoculation tube 19. The discharged microbial liquid initially falls on the liquid surface of the culture liquid in the container body 1; S53, control the driving motor 15 of the automatic dispersing component 9 to start, the driving motor 15 drives the connecting plate 16 to rotate around the axis of the driving motor 15, and the connecting plate 16 drives the peristaltic pump 24, the storage tank 23, the movable bar 17, the fixed plate 18, the spring 21, and the nylon wheel 22 thereon to rotate synchronously, wherein the nylon wheel 22 rotates relative to the axis of the annular ring 13 in a stationary state, and the rotation direction is as follows: Fig. 27 As shown by the solid arrow in the middle, during the rotation process, the nylon wheel 22 makes a wave-like motion along each arc groove 14, and the nylon wheel 22 making a wave-like motion drives the rod 20 to make a reciprocating radial motion relative to the annular ring 13 in a stationary state, and the rod 20 drives the movable bar 17 to make a reciprocating radial motion, and the movable bar 17 drives the inoculation tube 19 to make a reciprocating radial motion. When the nylon wheel 22 rotates one circle, the controller controls the drive motor 15 and the peristaltic pump 24 to be turned off; because the inoculation tube 19 rotates relative to the axis of the annular ring 13 in a stationary state, and makes a reciprocating radial motion relative to the annular ring 13 in a stationary state, the inoculation tube 19 makes a flower-shaped motion above the liquid surface of the culture liquid, and the microbial liquid flowing out from the bottom port of the inoculation tube 19 is dispersed in the culture liquid of the container body 1 in a flower shape, and finally the first batch of microbial liquid is dispersed in the culture liquid of the container body 1 in a flower shape, as shown in FIG. Figure 5 As shown; S6, the staff controls the stepper motor 27 of the fixed and rotating assembly 4 to start, the stepper motor 27 drives the arch frame A, the turntable 28 and the suction cup A30 to rotate synchronously, and the turntable 28 drives the container body 1 to rotate synchronously. The rotation direction of the container body 1 is as follows: Fig.28As shown by the hollow arrow, when the container body 1 rotates to the set angle, the controller controls the stepping motor 27 to turn off; then the staff repeats the operations of steps S52 to S53, and the second microbial liquid can be dispersed in the culture liquid of the container body 1 in a flower shape; S7, the staff repeats the operation of step S6 for multiple times, and can disperse multiple batches of microbial liquid in the culture liquid of the container body 1 in a flower shape, such as Figure 6 As shown; S8, re-covering the plastic cover 2 in the automatic cover removal assembly 8 on the top port of the container body 1, and the specific operation steps are as follows: S81, control the servo motor 5 to start, the servo motor 5 drives the main shaft 6 to rotate, the main shaft 6 drives the strip plate 7 to rotate, the strip plate 7 drives the automatic cover removal component 8 and the automatic dispersion component 9 to rotate synchronously, the automatic cover removal component 8 drives the removed plastic cover 2 to rotate synchronously, when the strip plate 7 rotates 180°, the controller controls the servo motor 5 to turn off, at this time, the plastic cover 2 in the automatic cover removal component 8 just moves to the top of the container body 1; S82, control the piston rod of the cover removal cylinder 33 of the automatic cover removal assembly 8 to extend downward, and when the piston rod of the cover removal cylinder 33 is fully extended, the plastic cover 2 is re-covered on the container body 1, as shown in FIG. Fig.29 As shown, the top port of the container body 1 is sealed, thereby finally achieving the inoculation of the microbial liquid in the large-sized container; S9. Removal of the large-sized container inoculated with the microbial liquid, the specific operation steps are as follows: S91, control the piston rods of the two horizontal cylinders 37 of the automatic cover removal assembly 8 to extend outward, the piston rods drive the vertical plate to move outward, the vertical plate drives the lifting plate 38 to move outward, and the semicircular hole 39 of the lifting plate 38 is separated from the container body 1; then control the vacuum pump B36 to turn off, at this time, the suction cup B34 no longer absorbs the top of the plastic cover 2; then control the piston rod of the cover removal cylinder 33 to retract upward, and the piston rod drives the suction cup B34 to reset; S92, control the vacuum pump A32 to turn off, at this time, the suction cup A30 no longer adsorbs the container body 1; then the staff will take away the large-sized container inoculated with the microbial liquid, and the direction of taking away is as follows Fig.30 As indicated by the arrow; S10. Repeat steps S1 to S9 for multiple times, and then multiple large-sized containers can be inoculated with microbial liquid.

[0028] Among them, from step S5, the staff only needs to make the lifting cylinder 10 and the driving motor 15 of the automatic dispersion component 9 to make the inoculation tube 19 rotate relative to the axis of the annular ring 13 in a stationary state, and make reciprocating radial motion relative to the annular ring 13 in a stationary state, so that the inoculation tube 19 makes a flower-shaped motion above the liquid surface of the culture solution, and then the microbial liquid flowing out from the bottom port of the inoculation tube 19 is dispersed in the culture solution of the container body 1 in a flower shape, and finally the first batch of microbial liquid is dispersed in the culture solution of the container body 1 in a flower shape; then in steps S6~S7, only through the linkage cooperation of the automatic dispersion component 9 and the fixing and rotating component 4, it is possible to disperse multiple batches of microbial liquid in the culture solution of the container body 1 in a flower shape.

[0029] It can be seen that compared with the traditional inoculation method, the present inoculation device does not require the staff to use the inoculator to extract a certain amount of microbial liquid from the storage tank, nor does it require the staff to manually move the inoculator in a flower shape to disperse the microbial liquid in the culture liquid of the container body 1 in a flower shape. Instead, the microbial liquid is automatically dispersed in the culture liquid of the container body 1 in a flower shape, thereby achieving the goal of dispersing multiple batches of microbial liquid in the culture liquid in a flower shape in a short time, thereby greatly improving the inoculation efficiency of the microbial liquid.

Claims

1. An inoculation device for efficiently inoculating a microbial solution in a large-sized container, characterized in that: It comprises a fixing and rotating assembly (4) arranged on a workbench (3) for fixing and driving a large-sized container to rotate around its own axis, a servo motor (5) fixedly arranged on the bottom surface of the workbench (3), the output shaft of the servo motor (5) passing through the workbench (3) upwards, and a main shaft (6) is connected to the extended end, a strip plate (7) is fixedly arranged on the top of the main shaft (6), an automatic cover removal assembly (8) for removing a plastic cover (2) of the large-sized container is arranged on the left end of the strip plate (7), the automatic cover removal assembly (8) is located directly above the fixing and rotating assembly (4), and an automatic dispersing assembly (9) for dispersing a microbial liquid in a flower shape in a container body (1) of the large-sized container is arranged on the right end of the strip plate (7); The automatic dispersion component (9) comprises a lifting cylinder (10) fixedly mounted on the right end of the strip plate (7), the piston rod of the lifting cylinder (10) penetrating downwardly through the strip plate (7), and a lifting plate (11) is fixedly mounted on the extended end, an L plate (12) is fixedly mounted on the bottom surface of the left end of the lifting plate (11), an annular ring (13) is fixedly mounted on the end portion of the L plate (12), a plurality of arc grooves (14) arranged at intervals are formed on the inner wall of the center hole of the annular ring (13) along its circumference, a driving motor (15) is fixedly mounted on the bottom surface of the right end of the lifting plate (11), and a connecting plate (16) penetrating downwardly through the center hole of the annular ring (13) is fixedly mounted on the output shaft of the driving motor (15); A movable bar (17) extending to the right is slidably penetrated in the lower end of the connecting plate (16), and a fixed plate (18), an inoculation tube (19) and a rod (20) are fixedly arranged on the top surface of the movable bar (17) in sequence from left to right, and a spring (21) is fixedly arranged between the fixed plate (18) and the connecting plate (16); the top end of the inoculation tube (19) penetrates the central hole of the annular ring (13) upward and extends above the annular ring (13), and a nylon wheel (22) is rotatably installed on the top end of the rod (20), and under the elastic force of the spring (21), the nylon wheel (22) abuts against the bottom of the rightmost arc groove (14) in the annular ring (13); A storage tank (23) storing microbial liquid is fixedly provided on the left end surface of the connecting plate (16), and a peristaltic pump (24) is fixedly provided on the bottom surface of the connecting plate (16). A liquid extraction pipe (25) is connected to the liquid extraction port of the peristaltic pump (24), and the liquid extraction pipe (25) passes through the connecting plate (16) and is connected to the bottom port of the storage tank (23). A liquid discharge pipe (26) is connected to the liquid discharge port of the peristaltic pump (24), and the liquid discharge pipe (26) is connected to the top port of the inoculation tube (19).

2. The inoculation device for efficiently inoculating microbial liquid in a large-sized container according to claim 1, characterized in that: The axis of the drive motor (15) is coaxial with the axis of the annular ring (13).

3. The inoculation device for efficiently inoculating microbial liquid in a large-sized container according to claim 2, characterized in that: The arc-shaped grooves (14) are evenly distributed on the inner wall of the annular ring (13).

4. The inoculation device for efficiently inoculating microbial liquid in a large-sized container according to claim 3, characterized in that: A support plate is fixedly arranged on the left end surface of the connecting plate (16), and the storage tank (23) is fixedly arranged on the support plate.

5. The inoculation device for efficiently inoculating microbial liquid in a large-sized container according to claim 4, characterized in that: The fixed and rotating assembly (4) comprises a stepper motor (27) fixedly mounted on the surface of a workbench (3), and an arch frame A fixedly mounted on the output shaft of the stepper motor (27); a turntable (28) is fixedly mounted on the top of the arch frame A; a positioning hole (29) is provided on the top surface of the turntable (28); the diameter of the positioning hole (29) is equal to the outer diameter of the container body (1) of the large-sized container; a step hole penetrating the bottom surface of the turntable (28) is provided at the bottom of the positioning hole (29); a suction cup (30) is fixedly mounted in the large hole of the step hole; the top surface of the suction cup A (30) is flush with the bottom of the positioning hole (29); a plurality of vacuum holes A (31) connected to the inner cavity of the suction cup A (30) are provided on the top surface of the suction cup A (30); a vacuum pump A (32) is fixedly mounted in the arch frame A; a working port of the vacuum pump A (32) is connected to the inner cavity of the suction cup A (30) via a pipeline.

6. The inoculation device for efficiently inoculating microbial liquid in a large-sized container according to claim 5, characterized in that: The automatic cover removal assembly (8) comprises a cover removal cylinder (33) fixedly mounted on the left end of the strip plate (7); a piston rod of the cover removal cylinder (33) penetrates downward through the strip plate (7), and an arch frame B is fixedly mounted on the extended end; a suction cup B (34) is fixedly mounted on the bottom surface of the arch frame B; a plurality of vacuum holes B (35) connected to the inner cavity of the suction cup B (34) are formed on the bottom surface of the suction cup B (34); a vacuum pump B (36) is fixedly mounted in the arch frame B; a working port of the vacuum pump B (36) is connected to the inner cavity of the suction cup B (34); A horizontal cylinder (37) is fixedly provided on the top surface of the suction cup B (34) and on the left and right sides thereof. The piston rods of the two horizontal cylinders (37) extend outside the suction cup B (34), and a vertical plate is fixedly provided on the extended end. A longitudinally arranged lifting plate (38) is fixedly provided on the inner end surfaces of the two vertical plates. A semicircular hole (39) is opened on the inner end surfaces of the two lifting plates (38), and the diameter of the semicircular hole (39) is equal to the outer diameter of the container body (1) of the large-sized container.

7. The inoculation device for efficiently inoculating microbial liquid in a large-sized container according to claim 6, characterized in that: A plurality of legs supported on the ground are fixedly arranged on the bottom surface of the workbench (3).

8. The inoculation device for efficiently inoculating microbial liquid in a large-sized container according to claim 7, characterized in that: The inoculation device also includes a controller, which is electrically connected to the servo motor (5), the drive motor (15), the stepper motor (27), the lifting cylinder (10), the cover removal cylinder (33), the horizontal cylinder (37), the peristaltic pump (24), the vacuum pump A (32), and the vacuum pump B (36) via signal lines.

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