Fermented enzymatically hydrolyzed palm fiber, preparation method thereof, Pleurotus geesteranus culture medium and application thereof

Abstract

The present invention relates to the field of microbial technology, and in particular, to a fermented enzymatically hydrolyzed palm filament, a preparation method thereof, a Pleurotus geesteranus culture medium and applications. The method for preparing the fermented enzymatically hydrolyzed palm filament comprises the following steps: subjecting a mixed solution containing palm filaments, a fermentation agent and a fermentation enzyme agent to anaerobic fermentation to obtain the fermented enzymatically hydrolyzed palm filaments; wherein the fermentation agent comprises: yeast and lactic acid bacteria; the fermentation enzyme agent comprises: cellulase, xylanase and mannanase. The method for preparing the fermented enzymatically hydrolyzed palm filaments, wherein the fermented enzymatically hydrolyzed palm filaments prepared can be used as one of the main raw materials of the Pleurotus geesteranus culture medium, which can not only reasonably utilize the palm filaments to reduce pollution, but also expand the types of raw materials for the cultivation of Pleurotus geesteranus, save the cultivation cost of Pleurotus geesteranus, and increase the yield of Pleurotus geesteranus.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms, and in particular to a fermentation-enzymatically hydrolyzed palm fiber and a preparation method thereof, a Pleurotus geesteranus culture medium and an application thereof. Background Art

[0002] Oil palm, known as the "king of oil in the world", is an oil crop. Its pulp and kernels are rich in oil, with high oil yield and high economic value. It is mainly distributed in Indonesia, Malaysia, Africa and other places. my country also began to plant oil palm in Guangdong, Yunnan, Hainan and other provinces in 1926. Palm fiber is a by-product obtained after the pulp and kernels of oil palm are squeezed for oil. It pollutes the environment very much. Countries are developing new technologies to utilize palm fiber.

[0003] Pleurotus geesteranus is a kind of oyster mushroom, scientifically known as Lentinus edodes, belonging to Fungi, Basidiomycetes, Agaricales, Pleurotus, and is one of the rare edible mushrooms with a wide cultivation area in recent years. In the cultivation process of Pleurotus geesteranus, cottonseed hulls and sawdust are generally used as cultivation raw materials, but cottonseed hulls have high pesticide residues, and the organic edible fungi standard clearly stipulates that cottonseed hulls cannot be used as cultivation raw materials. Currently, the cultivation raw materials of organic Pleurotus geesteranus are bamboo shoot hulls and sawdust, but the yield is only 40% to 60% of the yield of cottonseed hull cultivation.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] The purpose of the present invention is to provide a fermented enzymatic palm fiber and a preparation method thereof, a Pleurotus geesteranus culture medium and an application thereof. The preparation method of the fermented enzymatic palm fiber can be used as one of the main raw materials of the Pleurotus geesteranus culture medium, which can not only reasonably utilize the palm fiber to reduce pollution, but also expand the types of cultivation raw materials of Pleurotus geesteranus, save the cultivation cost of Pleurotus geesteranus, and increase the yield of Pleurotus geesteranus.

[0006] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0007] One aspect of the present invention relates to a method for preparing fermentation-enzymatically hydrolyzed palm fibers, comprising the following steps:

[0008] Anaerobic fermentation of a mixed solution containing palm fibers, fermentation bacteria and fermentation enzymes to obtain the fermented enzymatically hydrolyzed palm fibers;

[0009] Wherein, the fermentation bacteria include: yeast and lactic acid bacteria;

[0010] The fermentation enzymes include cellulase, xylanase and mannanase.

[0011] The method for preparing fermented enzymatically hydrolyzed palm fibers can be used as one of the main raw materials of the Pleurotus geesteranus culture medium, which can not only reasonably utilize the palm fibers to reduce pollution, but also expand the types of Pleurotus geesteranus cultivation raw materials, save the cultivation cost of Pleurotus geesteranus, and increase the yield of Pleurotus geesteranus.

[0012] Another aspect of the present invention also relates to the fermented enzymatically hydrolyzed palm fibers prepared by the method for preparing the fermented enzymatically hydrolyzed palm fibers.

[0013] The fermented enzymatically hydrolyzed palm fibers can be used as one of the main raw materials of the culture medium of Pleurotus geesteranus, which can not only reasonably utilize the palm fibers to reduce pollution, but also expand the types of cultivation raw materials of Pleurotus geesteranus, save the cultivation cost of Pleurotus geesteranus, and increase the yield of Pleurotus geesteranus.

[0014] Another aspect of the present invention also relates to a Pleurotus geesteranus culture medium, which comprises the following components in parts by mass:

[0015] The fermentation and enzymatic hydrolysis comprises 45-55 parts of palm silk, 10-20 parts of corn cobs, 10-15 parts of sawdust, 15-25 parts of bran, 0.5-2 parts of lime, 0.5-2 parts of superphosphate and 0.5-2 parts of molasses.

[0016] The Pleurotus geesteranus cultivated by using the Pleurotus geesteranus culture medium has good mycelium growth, fast growth speed, high mushroom yield, and reduced Pleurotus geesteranus cultivation cost. The fermented palm fibers can well replace cottonseed hulls and are safer.

[0017] Another aspect of the present invention also relates to a method for cultivating Pleurotus geesteranus, wherein the Pleurotus geesteranus strain is inoculated into the Pleurotus geesteranus culture medium for cultivation.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] (1) The method for preparing fermented enzymatically hydrolyzed palm fibers provided by the present invention utilizes specific fermentation bacteria and fermentation enzymes to perform anaerobic fermentation on palm fibers. The obtained fermented enzymatically hydrolyzed palm fibers can be used as one of the main raw materials for the culture medium of Pleurotus geesteranus. This method can not only reasonably utilize palm fibers to reduce pollution, but also expand the types of raw materials for the cultivation of Pleurotus geesteranus, save the cultivation cost of Pleurotus geesteranus, and increase the yield of Pleurotus geesteranus.

[0020] (2) The fermented enzymatically hydrolyzed palm fiber provided by the present invention can be used as one of the main raw materials of the culture medium of Pleurotus geesteranus. It can not only reasonably utilize the palm fiber to reduce pollution, but also expand the types of cultivation raw materials of Pleurotus geesteranus, save the cultivation cost of Pleurotus geesteranus, and increase the yield of Pleurotus geesteranus.

[0021] (3) The culture medium for Pleurotus geesteranus provided by the present invention uses fermented palm fibers as the main raw material and adds other auxiliary materials of certain types and amounts. The Pleurotus geesteranus cultivated using the culture medium has good mycelium growth, fast growth rate, and high mushroom yield, thereby reducing the cultivation cost of Pleurotus geesteranus. Fermented palm fibers can be a good substitute for cottonseed hulls and are safer. DETAILED DESCRIPTION

[0022] The technical scheme of the present invention will be clearly and completely described below in conjunction with specific embodiments, but it will be appreciated by those skilled in the art that the following described embodiments are part of embodiments of the present invention, rather than all embodiments, and are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work, all belong to the scope of protection of the present invention. If specific conditions are not indicated in the embodiments, they are carried out according to normal conditions or conditions recommended by the manufacturer. If the manufacturer is not indicated in the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0023] One aspect of the present invention relates to a method for preparing fermentation-enzymatically hydrolyzed palm fibers, comprising the following steps:

[0024] Anaerobic fermentation of a mixed solution containing palm fibers, fermentation bacteria and fermentation enzymes to obtain the fermented enzymatically hydrolyzed palm fibers;

[0025] Wherein, the fermentation bacteria include: yeast and lactic acid bacteria;

[0026] The fermentation enzymes include cellulase, xylanase and mannanase.

[0027] The method for preparing fermented enzymatically hydrolyzed palm fibers utilizes specific fermentation bacteria and fermentation enzymes to perform anaerobic fermentation on the palm fibers. The obtained fermented enzymatically hydrolyzed palm fibers can be used as one of the main raw materials for the culture medium of Pleurotus geesteranus. The method can not only reasonably utilize the palm fibers to reduce pollution, but also expand the types of raw materials for cultivating Pleurotus geesteranus, save the cultivation cost of Pleurotus geesteranus, and increase the yield of Pleurotus geesteranus.

[0028] Furthermore, yeast (1-5)×10 7 CFU / g and lactic acid bacteria (1~5)×10 6 CFU / g. Adding a certain amount of yeast and lactic acid bacteria to palm fibers can better perform anaerobic fermentation on the palm fibers, and the nutrients contained in the anaerobic fermentation products obtained are suitable for the growth of Pleurotus geesteranus.

[0029] In some specific embodiments, the amount of yeast added per gram of palm fiber can be, for example, but not limited to, 1×10 7 CFU / g, 2×107 CFU / g, 3×10 7 CFU / g, 4×10 7 CFU / g or (1~5)×10 7 CFU / g or a range of values ​​between them.

[0030] In some specific embodiments, the amount of lactic acid bacteria added per gram of palm fiber can be, for example, but not limited to, 1×10 6 CFU / g, 2×10 6 CFU / g, 3×10 6 CFU / g, 4×10 6 CFU / g or 5×10 6 CFU / g or a range of values ​​between them.

[0031] Furthermore, 50-200 U / g of cellulase, 50-200 U / g of xylanase and 50-200 U / g of mannanase are added to each gram of palm fiber. Adding a certain amount of cellulase, xylanase and mannanase to the palm fiber can fully enzymolyze the palm fiber to obtain small molecules that can be utilized, thereby improving the utilization rate of the palm fiber and the compatibility of the fermentation product with the cultivation of Pleurotus geesteranus.

[0032] In some specific embodiments, the amount of cellulase added per gram of palm fiber can be, for example, but not limited to, any one of 50 U / g, 70 U / g, 90 U / g, 110 U / g, 130 U / g, 150 U / g, 170 U / g, 190 U / g or 200 U / g, or a range between any two of them.

[0033] In some specific embodiments, the amount of xylanase added per gram of palm fiber can be, for example, but not limited to, any one of 50 U / g, 70 U / g, 90 U / g, 110 U / g, 130 U / g, 150 U / g, 170 U / g, 190 U / g or 200 U / g, or a range between any two of them.

[0034] In some specific embodiments, the amount of mannanase added per gram of palm fiber can be, for example, but not limited to, any one of 50 U / g, 70 U / g, 90 U / g, 110 U / g, 130 U / g, 150 U / g, 170 U / g, 190 U / g or 200 U / g, or a range between any two of them.

[0035] Furthermore, the water content of the mixed solution containing palm fibers, fermentation bacteria and fermentation enzyme is 40% to 60%, including but not limited to any point value of 40%, 45%, 50%, 55% or 60% or any range value between two of them. Controlling the water content of the fermentation base material within a certain range is conducive to better performance of the corresponding functions of the fermentation bacteria and fermentation enzyme, and is conducive to the smooth progress of anaerobic fermentation.

[0036] Furthermore, the anaerobic fermentation time is 3 to 5 days, including but not limited to any point value of 3 days, 4 days or 5 days or a range value between any two of them.

[0037] Furthermore, the temperature of the anaerobic fermentation is 30-35°C, including but not limited to any point value of 30°C, 31°C, 32°C, 33°C, 34°C or 35°C or a range value between any two of them.

[0038] Anaerobic fermentation at a certain temperature and time can fully complete the fermentation of palm fibers, releasing a large amount of usable substances to supply the growth of Pleurotus geesteranus.

[0039] Another aspect of the present invention also relates to the fermented enzymatically hydrolyzed palm fibers prepared by the method for preparing the fermented enzymatically hydrolyzed palm fibers.

[0040] The fermented enzymatically hydrolyzed palm fibers can be used as one of the main raw materials of the Pleurotus geesteranus culture medium, which can not only reasonably utilize the palm fibers to reduce pollution, but also expand the types of Pleurotus geesteranus cultivation raw materials, save the cultivation cost of Pleurotus geesteranus, and increase the yield of Pleurotus geesteranus.

[0041] Another aspect of the present invention also relates to a Pleurotus geesteranus culture medium, which comprises the following components in parts by mass:

[0042] The fermentation and enzymatic hydrolysis comprises 45-55 parts of palm silk, 10-20 parts of corn cobs, 10-15 parts of sawdust, 15-25 parts of bran, 0.5-2 parts of lime, 0.5-2 parts of superphosphate and 0.5-2 parts of molasses.

[0043] In some specific embodiments, the fermentation enzymatic hydrolysis of palm fiber can be, for example, but not limited to, any one of 45 parts, 47 parts, 49 parts, 51 parts, 53 parts or 55 parts by weight, or a range between any two of them.

[0044] In some specific embodiments, the corncob may be, for example, but not limited to, any one of 10, 12, 14, 16, 18 or 20 parts by weight, or a range of values ​​between any two parts.

[0045] In some specific embodiments, the wood chips may be, for example, but not limited to, any one of 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, or 15 parts by mass, or a range of values ​​between any two of them.

[0046] In some specific embodiments, the bran may be, for example, but not limited to, any one of 15 parts, 17 parts, 19 parts, 21 parts, 23 parts or 25 parts by weight, or a range of values ​​between any two of them.

[0047] In some specific embodiments, the lime may be, for example, but not limited to, any one of 0.5, 0.7, 0.9, 1.1, 1.3, 1.5, 1.7, 1.9 or 2 parts by mass, or a range between any two parts.

[0048] In some specific embodiments, the superphosphate may be, for example, but not limited to, any one of 0.5, 0.7, 0.9, 1.1, 1.3, 1.5, 1.7, 1.9 or 2 parts by mass, or a range between any two parts.

[0049] In some specific embodiments, the molasses may be, for example, but not limited to, any one of 0.5, 0.7, 0.9, 1.1, 1.3, 1.5, 1.7, 1.9 or 2 parts by mass, or a range between any two parts.

[0050] The Pleurotus geesteranus culture medium uses fermented palm fibers as a main raw material and adds other auxiliary materials of a certain type and amount. The Pleurotus geesteranus cultivated using the culture medium has good mycelium growth, fast growth rate, high mushroom yield, and reduced Pleurotus geesteranus cultivation costs. The fermented palm fibers can well replace cottonseed hulls and are safer.

[0051] Furthermore, the Pleurotus geesteranus culture medium comprises the following components by weight:

[0052] The fermentation and enzymatic hydrolysis comprises 48-53 parts of palm silk, 12-18 parts of corn cobs, 11-14 parts of sawdust, 17-23 parts of bran, 0.8-1.8 parts of lime, 0.8-1.8 parts of superphosphate and 0.8-1.8 parts of molasses.

[0053] Another aspect of the present invention also relates to a method for cultivating Pleurotus geesteranus, wherein Pleurotus geesteranus strains are inoculated into the Pleurotus geesteranus culture medium for cultivation.

[0054] The present invention does not specifically limit the cultivation method of Pleurotus geesteranus. The Pleurotus geesteranus culture medium provided by the present invention is suitable for the conventional Pleurotus geesteranus cultivation method in the art.

[0055] The embodiments of the present invention will be described in detail below in conjunction with the examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. If no specific conditions are specified in the examples, the conditions are carried out according to normal conditions or the conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be obtained commercially.

[0056] Example 1

[0057] The method for preparing palm fiber by fermentation and enzymatic hydrolysis provided in this embodiment comprises the following steps:

[0058] Add 5*10 yeast to 1g of palm silk 7 CFU / g, lactic acid bacteria 5*10 6 CFU / g, cellulase 200U / g, xylanase 200U / g and mannanase 200U / g to obtain a fermentation base material, control the moisture content of the fermentation base material to 60%, perform anaerobic fermentation for 5 days at a fermentation temperature of 35°C to obtain fermented enzymatically hydrolyzed palm fibers.

[0059] Example 2

[0060] The method for preparing palm fiber by fermentation and enzymatic hydrolysis provided in this embodiment comprises the following steps:

[0061] Add 1*10 yeast to 1g of palm silk 7 CFU / g, lactic acid bacteria 1*10 6 CFU / g, cellulase 50 / g, xylanase 50U / g and mannanase 50U / g to obtain a fermentation base material, control the moisture content of the fermentation base material to 40%, perform anaerobic fermentation for 3 days, and ferment at a temperature of 30°C to obtain fermented enzymatically hydrolyzed palm fibers.

[0062] Example 3

[0063] The method for preparing palm fiber by fermentation and enzymatic hydrolysis provided in this embodiment comprises the following steps:

[0064] Add 2*10 yeast to 1g of palm silk 7 CFU / g, lactic acid bacteria 3*10 6 CFU / g, cellulase 100U / g, xylanase 100U / g and mannanase 100U / g to obtain a fermentation base material, control the moisture content of the fermentation base material to 48%, perform anaerobic fermentation for 4 days at a fermentation temperature of 33°C to obtain fermented enzymatically hydrolyzed palm fibers.

[0065] Example 4

[0066] The Pleurotus geesteranus culture medium provided in this embodiment includes the following components by weight:

[0067] The fermentation and enzymatic hydrolysis of Example 1 includes 50 parts of palm silk, 15 parts of corn cobs, 12 parts of sawdust, 20 parts of bran, 1 part of lime, 1 part of superphosphate and 1 part of molasses.

[0068] Example 5

[0069] The Pleurotus geesteranus culture medium provided in this embodiment includes the following components by weight:

[0070] The fermentation and enzymatic hydrolysis of Example 2 includes 55 parts of palm silk, 10 parts of corn cobs, 10 parts of sawdust, 25 parts of bran, 2 parts of lime, 0.5 parts of superphosphate and 2 parts of molasses.

[0071] Example 6

[0072] The Pleurotus geesteranus culture medium provided in this embodiment includes the following components by weight:

[0073] The fermentation and enzymatic hydrolysis of Example 3 includes 45 parts of palm silk, 20 parts of corn cobs, 15 parts of sawdust, 15 parts of bran, 0.5 parts of lime, 2 parts of superphosphate and 0.5 parts of molasses.

[0074] Comparative Example 1

[0075] The only difference between this comparative example and Example 1 is that yeast and lactic acid bacteria are not added, and the Pleurotus geesteranus culture medium of Example 4 is used.

[0076] Comparative Example 2

[0077] The only difference between this comparative example and Example 1 is that no cellulase, xylanase and mannanase were added, and the Pleurotus geesteranus culture medium of Example 4 was used.

[0078] Comparative Example 3

[0079] Unprocessed palm fiber.

[0080] Comparative Example 4

[0081] The difference between this comparative example and Example 1 is that 6×10 yeasts are added per gram of palm silk. 7 CFU / g and lactic acid bacteria 0.5×10 6 CFU / g, using the Pleurotus geesteranus culture medium of Example 4.

[0082] Comparative Example 5

[0083] The difference between this comparative example and Example 1 is that 250 U / g of cellulase, 30 U / g of xylanase and 220 U / g of mannanase are added per gram of palm fiber, and the Pleurotus geesteranus culture medium of Example 4 is used.

[0084] Comparative Example 6

[0085] The comparative example adopts the palm fiber fermentation enzyme agent of Example 1 and the Pleurotus geesteranus culture medium, which includes the following components by weight:

[0086] Fermented enzymatically hydrolyzed palm silk 60 parts, corn cobs 5 parts, sawdust 20 parts, bran 10 parts, lime 4 parts, superphosphate 4 parts and molasses 4 parts.

[0087] Experimental example

[0088] According to the formula of Pleurotus geesteranus culture medium provided in each embodiment and comparative example, after mixing all raw materials, the water content of the system was adjusted to 65%, and the bags were sterilized at 121℃ for 1h. After cooling, the Pleurotus geesteranus strains were inoculated. After inoculation, the bacteria bags were moved to the bacteria culture room for bacteria culture. The bacteria culture room needs to be protected from light, the bacteria culture temperature is 25℃, the relative humidity of the air is below 70%, and the bacteria are ventilated 1-2 times a day for 20 days. After the mycelium is full of bags, it is placed in a mushroom fruiting room at 20-25℃ with certain scattered light for bud induction and mushroom fruiting management. The growth status of Pleurotus geesteranus is shown in Table 1.

[0089] Table 1 Growth status of Pleurotus geesteranus in different treatment groups

[0090] Average mycelium growth rate (cm / d) First tide yield (g) Example 4 0.53 33.16 Example 5 0.50 32.05 Example 6 0.52 32.18 Comparative Example 1 0.35 15.65 Comparative Example 2 0.43 20.39 Comparative Example 3 0.28 11.23 Comparative Example 4 0.45 24.56 Comparative Example 5 0.47 27.83 Comparative Example 6 0.44 22.63

[0091] By comparing the results of Example 1 with those of Comparative Example 3, it can be seen that the fermented enzymatic palm silk prepared by the palm silk fermentation and enzymatic hydrolysis method provided by the present invention is applied to the cultivation of Pleurotus geesteranus, and its mycelium growth rate and first-wave yield are significantly higher than those of palm silk that has not been fermented, which proves that the fermented enzymatic palm silk provided by the present invention is helpful to improve the growth rate and yield of Pleurotus geesteranus.

[0092] Comparison of the results of Example 1 with those of Comparative Examples 1 and 2 shows that, in the preparation of fermentation-enzymatically hydrolyzed palm fibers, both the fermentation bacteria agent and the fermentation enzyme agent are indispensable, and the lack of either the fermentation bacteria agent or the fermentation enzyme agent will affect the overall effect.

[0093] Although the present invention has been illustrated and described with specific embodiments, it should be appreciated that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Those skilled in the art should understand that the technical solutions described in the above embodiments may be modified, or some or all of the technical features thereof may be replaced by equivalents without departing from the spirit and scope of the present invention. However, these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention. Therefore, this means that all such replacements and modifications within the scope of the present invention are included in the appended claims.

Claims

1. A method for preparing palm fiber by fermentation and enzymatic hydrolysis, characterized in that: The following steps are involved: Anaerobic fermentation of a mixed solution containing palm fibers, fermentation bacteria and fermentation enzymes to obtain the fermented enzymatically hydrolyzed palm fibers; Wherein, the fermentation bacteria include: yeast and lactic acid bacteria; The fermentation enzymes include cellulase, xylanase and mannanase.

2. The method for preparing palm fiber by fermentation and enzymolysis according to claim 1, characterized in that: Add yeast (1-5) × 10 per gram of palm fiber 7 CFU / g and lactic acid bacteria (1~5)×10 6 CFU / g.

3. The method for preparing palm fiber by fermentation and enzymatic hydrolysis according to claim 1, characterized in that: To each gram of palm fiber, 50-200 U / g of cellulase, 50-200 U / g of xylanase and 50-200 U / g of mannanase were added.

4. The method for preparing palm fiber by fermentation and enzymatic hydrolysis according to claim 1, characterized in that: The water content of the mixed liquid containing palm fibers, fermentation bacteria and fermentation enzyme is 40% to 60%.

5. The method for preparing palm fiber by fermentation and enzymatic hydrolysis according to claim 1, characterized in that: The anaerobic fermentation time is 3 to 5 days; And / or, the temperature of the anaerobic fermentation is 30-35°C.

6. The fermented enzymatically hydrolyzed palm fiber prepared by the method for preparing fermented enzymatically hydrolyzed palm fiber according to any one of claims 1 to 5.

7. A culture medium for Pleurotus geesteranus, characterized in that: According to the mass fraction, Includes the following components: 45-55 parts of fermented enzymatically hydrolyzed palm fibers as claimed in claim 6, 10-20 parts of corn cobs, 10-15 parts of sawdust, 15-25 parts of bran, 0.5-2 parts of lime, 0.5-2 parts of superphosphate and 0.5-2 parts of molasses.

8. The Pleurotus geesteranus culture medium according to claim 7, characterized in that The Pleurotus geesteranus culture medium comprises the following components by weight: The fermentation and enzymatic hydrolysis comprises 48-53 parts of palm silk, 12-18 parts of corn cobs, 11-14 parts of sawdust, 17-23 parts of bran, 0.8-1.8 parts of lime, 0.8-1.8 parts of superphosphate and 0.8-1.8 parts of molasses.

9. A method for cultivating Pleurotus geesteranus, characterized in that: The Pleurotus geesteranus strain is inoculated into the Pleurotus geesteranus culture medium according to claim 7 or 8 for cultivation.