An antibacterial and antiallergic fabric and its preparation method and application

Abstract

The application discloses an antibacterial and antiallergic fabric and a preparation method and application thereof, and belongs to the technical field of functional materials and functional textiles. The antibacterial and antiallergic fabric is prepared by embedding core material of Bacillus subtilis which can efficiently metabolize protease, embedding the core material with sodium alginate and sodium alginate-chitosan as secondary outer wall material, and embedding the core material with polyhydroxyalkanoate (PHA) and polylactic acid-glycolic acid copolymer (PLGA) as outer wall material, and then finishing the core material on the surface of the fabric. The antibacterial and antiallergic fabric has the characteristics of efficiently decomposing allergens and actively resisting bacteria. When allergens are adsorbed on the surface of the fabric, the metabolic products of probiotics can be targeted to decompose the sensitized sites, the adhesion of pathogenic bacteria is reduced, and the effect of actively killing bacteria is realized, so that long-term and efficient synergy of antiallergic and antibacterial is realized.

Description

Technical Field

[0001] This invention relates to an antibacterial and anti-allergic fabric, its preparation method, and its application, belonging to the field of functional materials and functional textiles technology. Background Technology

[0002] As living standards continue to improve, people are paying more attention to their living environment and increasingly emphasizing hygiene and health. Functional textiles are also gaining more and more attention. Antibacterial and hypoallergenic fabrics, closely related to people's lives, have become a key area of ​​focus.

[0003] Currently, conventional methods for preparing antibacterial and anti-allergic fabrics include impregnation coating, graft crosslinking, blending spinning, and in-situ growth. However, impregnation coating typically involves applying antibacterial and anti-allergic agents to fibers, yarns, fabrics, garments, or various textile products; the antibacterial agents only exist on the surface of the fibers and fabrics, and their antibacterial effect decreases significantly after repeated washing. Graft crosslinking involves chemical reactions, is complex to operate, and is costly. Blending spinning adds antibacterial and anti-allergic agents to the fiber matrix through blending, solution dispersion, or masterbatch addition, resulting in good and durable antibacterial effects that are less prone to detachment and loss during post-processing and use; however, the preparation technology is highly challenging, especially regarding the antibacterial agents. In-situ growth directly reduces metal nano-ions on the matrix, focusing on the in-situ synthesis of metal nanoparticles, but requires sophisticated operation. Therefore, finding a simple and durable method for preparing antibacterial and anti-allergic fabrics is a pressing issue that needs to be addressed.

[0004] Furthermore, probiotics and their metabolites can disrupt the genetic material or important pathways of pathogenic bacteria, thus exerting excellent antibacterial functions. As natural biological antibacterial agents, probiotics have good biocompatibility and can also improve human immune function, making them an ideal alternative to current chemical antibacterial agents. However, the biggest drawback of probiotics is their short viable lifespan and susceptibility to colonization environment. Maintaining their long-term survival rate and biological activity remains a challenge for the probiotic industry. Therefore, finding a material that can effectively block exogenous allergens while possessing antibacterial properties is urgently needed.

[0005] Microencapsulation technology utilizes natural or synthetic polymers to encapsulate solid, liquid, or gaseous microcapsules, forming semi-permeable or sealed membranes. Protecting probiotics using microencapsulation technology is currently a hot research topic both domestically and internationally, and is considered one of the most efficient and economical methods for protecting probiotic activity. It can protect bacteria from damage by the external environment, improve the survival rate of probiotics during production, processing, and storage, and achieve controlled release of probiotics. For example, CN 118511879 A discloses a method for preparing probiotic antibacterial microcapsules, which uses an emulsification method to encapsulate freeze-dried probiotic powder in aminated lignin to obtain probiotic antibacterial microcapsules. However, the copper ions complexed on the surface of these microcapsules pose a risk of inducing protein toxicity stress leading to cell death; the cytotoxicity and safety have not been investigated. CN117926592A discloses a probiotic nonwoven fabric and its preparation method and application. It uses polyvinyl alcohol in the presence of a catalyst to combine with tetraethyl orthosilicate to form a copolymer system with polydimethylsiloxane. This copolymer system can form an interpenetrating network system with polymer materials, thereby enabling the wall material structure of the probiotic microcapsules to form a good adhesion effect with the nonwoven fabric. However, the modification method of the wall material is complicated and the durability is poor.

[0006] In addition, the application of probiotic microcapsules in the textile field is still in its early stages, and the following technical issues need to be improved: First, improve the biocompatibility between the microcapsule wall material and the probiotic core material to enhance the activity of probiotics; second, improve the affinity between microcapsules and fabric fibers to avoid the adverse effects of using large amounts of adhesives on the physical and chemical properties and comfort of the fibers. Summary of the Invention

[0007] [Technical Issues]

[0008] The preparation of antibacterial and anti-allergic fabrics has problems such as short-lasting antibacterial and anti-allergic effects, complicated operation, and poor durability.

[0009] The preparation of probiotic microcapsules has problems such as harmful substances, complex modification, and poor adhesion performance.

[0010] [Technical Solution]

[0011] To address the aforementioned problems, this invention provides an antibacterial and anti-allergic fabric, its preparation method, and its applications. The antibacterial and anti-allergic fabric prepared by this invention can achieve exogenous adsorption and decomposition of allergens while possessing long-lasting antibacterial properties, thus preventing harm to the human body from external allergens and harmful bacteria.

[0012] The first objective of this invention is to provide a method for preparing antibacterial and anti-allergic probiotic microcapsules, comprising the following steps:

[0013] (1) Wash and resuspend the activated probiotic solution to obtain a probiotic resuspension.

[0014] (2) Add the sub-outer wall material solution to the probiotic resuspension, mix well to form an aqueous phase; add the oil phase to the aqueous phase, stir, add the crosslinking agent, stir, centrifuge, wash, and obtain single-wall microcapsules; wherein, the sub-outer wall material is one or two of sodium alginate and sodium alginate-chitosan.

[0015] (3) Disperse the single-walled microcapsules in water, add the outer wall material solution, evaporate by rotary evaporation, centrifuge, wash and freeze dry to obtain double-walled microcapsules; wherein the outer wall material is one or two of polyhydroxyalkanoate (PHA) and polylactic acid-glycolic acid copolymer (PLGA).

[0016] In one embodiment of the present invention, the probiotic in step (1) is Bacillus subtilis subsp. BNCC132861.

[0017] In one embodiment of the present invention, prebiotics may be added to the probiotic resuspension in step (1). The prebiotics are one or more of inulin, fucoidan, and α-lactalbumin. The ratio of prebiotics to probiotic resuspension is 1-3g:100mL.

[0018] In one embodiment of the present invention, the method for preparing the activated probiotic solution in step (1) is as follows:

[0019] The frozen probiotic strains were activated at 30-37℃ for 12-24 hours; then placed in beef extract peptone medium (BPM) and cultured at 30-37℃ and 50-200 rpm for 12-24 hours, with an inoculum size of 0.5-1.5% (v / v).

[0020] In one embodiment of the present invention, the washing and resuspension of the activated probiotic solution in step (1) is performed using PBS solution.

[0021] In one embodiment of the present invention, the concentration of probiotics in the probiotic resuspension in step (1) is 10. 8 -10 10 CFU / mL.

[0022] In one embodiment of the present invention, the secondary outer wall material solution in step (2) is an aqueous solution of the secondary outer wall material with a mass concentration of 1-3 wt%, and the outer wall material solution needs to be sterilized. The sterilization conditions are: sterilization at 121°C for 20 min.

[0023] In one embodiment of the present invention, the volume ratio of probiotic resuspension to sub-outer wall material solution in step (2) is 1:100-10:100.

[0024] In one embodiment of the present invention, the oil phase in step (2) is a mixture of vegetable oil and Tween 80, wherein the vegetable oil includes one or more of soybean oil, coconut oil, and palm oil; and the mass ratio of vegetable oil to Tween 80 is 100:0.5-2.5.

[0025] In one embodiment of the present invention, the volume ratio of the aqueous phase, oil phase and crosslinking agent in step (2) is 1:2:1-1:3:1.

[0026] In one embodiment of the present invention, the crosslinking agent in step (2) is an aqueous solution of CaCl2 with a concentration of 0.05-0.5M.

[0027] In one embodiment of the present invention, the stirring in step (2) is carried out at 20-30°C and 400-1200 rpm for 30-70 minutes.

[0028] In one embodiment of the present invention, in step (2), centrifugation and washing are performed by centrifuging at 3000-5000 rpm for 5-15 minutes, discarding the upper oil phase and the lower water phase, and washing with water.

[0029] In one embodiment of the present invention, the solvent of the outer wall material solution in step (3) is ethyl acetate with a concentration of 3-5 wt%.

[0030] In one embodiment of the present invention, the ratio of single-walled microcapsules, water and outer wall material solution in step (3) is 1g:8-12mL:100mL, and more preferably 1g:10mL:100mL.

[0031] In one embodiment of the present invention, the rotary evaporation in step (3) is carried out at 35-45°C and a vacuum of 400-600 mbar for 10-45 min.

[0032] In one embodiment of the present invention, in step (3), centrifugation and washing are performed by centrifuging at 3000-5000 rpm for 5-15 min, discarding the supernatant, and washing with water.

[0033] In one embodiment of the present invention, freeze drying in step (3) is performed at -20 to -40°C for 24-48 hours.

[0034] The second objective of this invention is to prepare antibacterial and anti-allergic probiotic microcapsules using the method described in this invention.

[0035] A third objective of this invention is to provide a method for preparing antibacterial and anti-allergic fabrics, comprising the following steps:

[0036] Unsized cotton fabric is placed in an alkaline solution, boiled, degreased and impurities are removed, and surface hydroxyl groups are introduced to obtain pretreated cotton fabric.

[0037] Antibacterial and anti-allergic probiotic microcapsules were dispersed in a cationic solution, and a cross-linking agent was added to obtain a cross-linking agent solution;

[0038] The pretreated cotton fabric is placed in a crosslinking agent solution for crosslinking; after the crosslinking is completed, it is washed and dried to obtain an antibacterial and anti-allergic fabric.

[0039] In one embodiment of the present invention, the alkaline solution is a NaOH aqueous solution with a mass concentration of 1-3%; boiling is carried out at 85-95°C for 20-40 minutes.

[0040] In one embodiment of the present invention, the cationic solution is an aqueous solution of polydiallyldimethylammonium chloride with a mass fraction of 0.1-0.5 wt%.

[0041] In one embodiment of the present invention, the crosslinking agent is citric acid, and the mass fraction of the crosslinking agent in the cationic solution is 0.5-1%.

[0042] In one embodiment of the present invention, the mass ratio of antibacterial and anti-allergic probiotic microcapsules to cationic solution is 1-2:100.

[0043] In one embodiment of the present invention, the mass ratio of the pretreated cotton fabric to the crosslinking agent solution is 1:10-100.

[0044] In one embodiment of the present invention, crosslinking is performed at 20-30°C (room temperature) for 20-40 minutes, and can be repeated 1-3 times.

[0045] In one embodiment of the present invention, washing is performed using water.

[0046] In one embodiment of the present invention, drying is performed at 30-45°C.

[0047] The fourth objective of this invention is to prepare antibacterial and anti-allergic fabrics using the method described in this invention.

[0048] The fifth objective of this invention is to provide a method for improving the antibacterial and anti-allergic properties of cotton fabrics, which utilizes the antibacterial and anti-allergic fabrics described in this invention.

[0049] The sixth object of the present invention is to provide a garment fabric that employs the antibacterial and anti-allergic fabric described in the present invention.

[0050] The seventh objective of this invention is the application of the antibacterial and anti-allergic fabrics described herein in clothing textiles.

[0051] [Beneficial Effects]

[0052] (1) The method for preparing antibacterial and anti-allergic fabrics described in this invention has a short process flow, high production efficiency, and avoids the negative impact of inorganic and organic antibacterial finishing agents on the balance of human flora.

[0053] (2) The outer wall material of the microcapsule used in this invention has self-degradability. Its degradation products can inhibit the proliferation of harmful bacteria by adjusting the pH. The outermost layer of the microcapsule can effectively balance primary brittleness and secondary toughness by adjusting the degree of crosslinking. While providing long-term protection for the core material, it can also release probiotics from the microcapsules on the fabric surface through friction or washing, and colonize the fabric surface to achieve long-term antibacterial and anti-allergic effects.

[0054] (3) This invention uses Bacillus subsp. ... Detailed Implementation

[0055] The preferred embodiments of the present invention are described below. It should be understood that the embodiments are for better explanation of the present invention and are not intended to limit the present invention.

[0056] Test method:

[0057] 1. Testing of microcapsule yield and encapsulation efficiency:

[0058]

[0059] 2. Breathability test:

[0060] GB / T 5453-1997 "Determination of air permeability of textile fabrics".

[0061] 3. Mechanical property testing:

[0062] GB / T 3923.1-2013 Textiles - Tensile properties of fabrics - Part 1: Determination of breaking strength and elongation at break - Bar method.

[0063] 4. Antibacterial performance test:

[0064] GB / T 20944.3-2008 Evaluation of antimicrobial properties of textiles - Part 3: Vibration method.

[0065] 5. Anti-allergy performance test:

[0066] Enzyme-linked immunosorbent assay (ELISA):

[0067] The Der F 1 kit (InBio) was used for testing. A 5×5cm fabric sample was placed in a sealed bag, 1mL of test solution was added, and the sample was squeezed out. 100μL of the test solution was added to each test well. The control well was incubated with test solution that had not reacted with the fabric at 300rpm for 1h at room temperature. The liquid was discarded, the wells were washed, and a mixture of secondary antibody and label was added. The mixture was incubated at 300rpm for 1h at room temperature in the dark. 100μL of TMB solution was added to each well, and the color development was carried out for 10-25min. The stop solution was added, and the absorbance was measured. The amount of antigen in each well was determined according to the standard curve.

[0068] The formula for calculating anti-allergy properties is as follows:

[0069]

[0070] Raw materials used in the examples:

[0071] Polyhydroxyalkanoate PHA:RG, CAS:26744-04-7, purchased from Adamas.

[0072] Polylactic acid-glycolic acid copolymer (PLGA): CAS: 30846-39-0, purchased from Macklin.

[0073] Sodium alginate: RG, CAS: 9005-38-3, purchased from Adamas.

[0074] Chitosan: RG, CAS: 9012-76-4, purchased from Adamas.

[0075] Preparation method of sodium alginate-chitosan solution:

[0076] A 2 wt% sodium alginate aqueous solution and a 2 wt% chitosan aqueous solution were mixed at a volume ratio of 1:1 to obtain a homogeneous composite solution. The solution was sterilized by incubation at 121℃ for 20 minutes and then cooled to room temperature for later use.

[0077] Inulin: BC, CAS: 9005-80-5, purchased from Aladdin.

[0078] Fucoidan: RG, CAS: 9072-19-9, purchased from Adamas.

[0079] Bacillus subtilis: Bacillus subtilis subsp. BNCC132861.

[0080] Beef extract peptone medium (BPM): purchased from Qingdao Haibo Biotechnology.

[0081] Preparation method of activated probiotic bacterial solution:

[0082] The frozen probiotic strain (Bacillus subtilis subtilis BNCC132861) was activated at 37°C for 24 h; then it was placed in beef extract peptone medium (BPM) and cultured at 37°C and 120 rpm for 24 h, with an inoculum size of 1% (v / v).

[0083] PBS solution: purchased from Bikman Biotechnology Co., Ltd.

[0084] Water: Deionized water.

[0085] Unbleached cotton fabric: GB / T8629-2017 standard pure cotton fabric, 378g / m² 2 Purchased from Ningbo Textile Consumables and Instruments Center.

[0086] In the examples and comparative examples, solutions without a specific solvent are water; percentages without a specific meaning refer to mass percentages; and reaction conditions without a specific temperature refer to room temperature (20-30°C).

[0087] Example 1

[0088] A method for preparing antibacterial and anti-allergic probiotic microcapsules includes the following steps:

[0089] (1) The activated probiotic culture was washed once with PBS solution and resuspended in PBS solution to obtain a probiotic resuspension; wherein the concentration of probiotics in the probiotic resuspension was 10. 10 CFU / mL;

[0090] (2) Mix sodium alginate and water evenly, sterilize at 121℃ for 20 min, cool to room temperature, and obtain a sodium alginate aqueous solution with a mass fraction of 2%.

[0091] Soybean oil and Tween 80 are mixed evenly at a mass ratio of 100:1 to form an oil phase;

[0092] Add inulin to the probiotic resuspension and mix well. Then add 2% sodium alginate aqueous solution and mix well to form an aqueous phase. The ratio of probiotic resuspension, inulin, and sodium alginate aqueous solution is 5mL:3g:100mL.

[0093] An oil phase was added to the aqueous phase, and the mixture was stirred at 25°C and 600 rpm for 30 min. Then, a 0.2 M CaCl2 aqueous solution was added, and the mixture was stirred at 25°C and 600 rpm for 30 min. The mixture was then centrifuged at 5000 rpm for 10 min. The upper oil phase and the lower aqueous phase were discarded, and the mixture was washed with water to obtain single-walled microcapsules. The volume ratio of the aqueous phase, oil phase, and CaCl2 aqueous solution was 1:2:1.

[0094] (3) Mix polyhydroxy fatty acid ester (PHA) and ethyl acetate evenly to obtain a polyhydroxy fatty acid ester (PHA) solution with a mass fraction of 2 wt%.

[0095] Single-walled microcapsules were dispersed in water, and polyhydroxyalkanoate (PHA) solution was added. The mixture was rotary evaporated at 40°C and 500 mbar for 20 min, centrifuged at 5000 rpm for 10 min, the supernatant was discarded, and the mixture was washed with water. The mixture was then freeze-dried at -20°C for 48 h to obtain double-walled microcapsules. The ratio of single-walled microcapsules, water, and PHA solution was 1 g: 10 mL: 100 mL.

[0096] Example 2

[0097] In step (2) of Example 1, the inulin was changed to fucoidan, and the sodium alginate aqueous solution with a mass fraction of 2 wt% was changed to sodium alginate-chitosan solution; in step (3), the polyhydroxyalkanoate (PHA) solution with a mass fraction of 3 wt% was changed to a PHA / PLGA (mass ratio 1:1) solution with a total mass fraction of 2 wt%.

[0098] Everything else remained the same as in Example 1, resulting in double-walled microcapsules.

[0099] Example 3

[0100] The concentration of the CaCl2 aqueous solution in step (2) of Example 1 was adjusted to 0.05M, while other steps remained the same as in Example 1, to obtain double-walled microcapsules.

[0101] Example 4

[0102] The concentration of the CaCl2 aqueous solution in step (2) of Example 2 was adjusted to 0.05M, while other steps remained the same as in Example 2, to obtain double-walled microcapsules.

[0103] Example 5

[0104] The polyhydroxyalkanoate (PHA) solution with a mass fraction of 3 wt% in step (3) of Example 1 was changed to a PLGA solution with a mass fraction of 2 wt%; all other steps remained the same as in Example 1, and double-walled microcapsules were obtained.

[0105] Example 6

[0106] The sodium alginate aqueous solution with a mass fraction of 2 wt% in step (2) of Example 1 was adjusted to a sodium alginate-chitosan solution; all other steps remained the same as in Example 1, and double-walled microcapsules were obtained.

[0107] Example 7

[0108] Omit the inulin in step (2) of Example 1, and keep everything else the same as in Example 1 to obtain double-walled microcapsules.

[0109] Comparative Example 1

[0110] Step (3) of Example 1 is omitted, and everything else is kept the same as in Example 1 to obtain single-walled microcapsules.

[0111] Comparative Example 2

[0112] Step (3) of Example 2 is omitted, and everything else is kept the same as in Example 2 to obtain single-walled microcapsules.

[0113] Comparative Example 3

[0114] In step (2) of Example 1, sodium alginate was replaced with gelatin, while other steps remained the same as in Example 1, resulting in double-walled microcapsules.

[0115] Comparative Example 4

[0116] Step (3) of Example 1 is omitted, and the sodium alginate aqueous solution in step (2) is replaced with polyhydroxyalkanoate (PHA) solution. Everything else is the same as in Example 1 to obtain single-walled microcapsules.

[0117] The microcapsules obtained in the examples and comparative examples were subjected to performance tests, and the test results are as follows:

[0118] Table 1

[0119] example Yield (%) Encapsulation rate (%) Example 1 72.67 74.23 Example 2 68.85 73.47 Example 3 69.70 70.95 Example 4 62.68 69.60 Example 5 71.56 73.17 Example 6 70.21 72.04 Example 7 65.81 67.95 Comparative Example 1 75.78 71.05 Comparative Example 2 71.74 72.59 Comparative Example 3 68.33 63.14 Comparative Example 4 72.27 63.17

[0120] As can be seen from Table 1:

[0121] (1) The yield of single-walled microcapsules is slightly higher than that of double-walled microcapsules. This is because there is a certain loss during the coating process of the outer wall material.

[0122] (2) The yield and encapsulation rate of double-walled microcapsules with low cross-linking degree are lower than those of other double-walled microcapsules. This is because the low cross-linking degree makes microcapsules coated only with the outermost wall material prone to swelling and rupture during centrifugation, washing and secondary coating.

[0123] (3) The yield and encapsulation rate of the sample without added prebiotics were both low. This is because after adding prebiotics, probiotics can be adsorbed on the surface of prebiotics and are more easily encapsulated.

[0124] Example 8

[0125] A method for preparing antibacterial and anti-allergic fabrics includes the following steps:

[0126] Unsized cotton fabric was placed in a 2% NaOH aqueous solution and boiled at 90°C for 30 minutes to degrease and remove impurities, and to introduce surface hydroxyl groups to obtain pretreated cotton fabric.

[0127] The microcapsules prepared in the examples and comparative examples were dispersed in a 0.5 wt% aqueous solution of polydiallyldimethylammonium chloride, and citric acid was added to obtain a crosslinking agent solution; wherein the mass fraction of citric acid in the aqueous solution of polydiallyldimethylammonium chloride was 1%; the mass ratio of antibacterial and anti-allergic probiotic microcapsules to aqueous solution of polydiallyldimethylammonium chloride was 2:100;

[0128] The pretreated cotton fabric was placed in a crosslinking agent solution and crosslinked at 25°C for 30 minutes, and the process was repeated twice. After the process, the fabric was washed with water and air-dried to obtain an antibacterial and anti-allergic fabric. The mass ratio of the pretreated cotton fabric to the crosslinking agent solution was 1:10.

[0129] Comparative Example 5

[0130] A method for preparing antibacterial and anti-allergic fabrics includes the following steps:

[0131] (1) The activated probiotic culture was washed once with PBS solution and resuspended in PBS solution to obtain a probiotic resuspension; wherein the concentration of probiotics in the probiotic resuspension was 10. 10 CFU / mL;

[0132] (2) Place the unsized cotton fabric in a 2% NaOH aqueous solution and boil it at 90°C for 30 minutes to degrease and remove impurities, introduce surface hydroxyl groups, and obtain the pretreated cotton fabric.

[0133] (3) Place 4g of pretreated cotton fabric in 50mL of probiotic resuspension, dip and nibble twice, with a nibble rate of 93.31%, dry at 60℃ to remove excess moisture, and obtain the fabric.

[0134] The obtained fabric was subjected to performance testing, and the test results are as follows:

[0135] Table 2

[0136] Microcapsules Air permeability (mm / s) Meridional elongation at break (%) Weft yarn breaking elongation (%) Unsized cotton fabrics 87.52 18.05 19.53 Example 1 80.61 18.95 19.21 Example 2 81.09 19.03 19.03 Example 3 82.12 19.24 19.37 Example 4 81.27 18.92 18.96 Example 5 80.11 19.31 19.01 Example 6 82.37 18.89 18.47 Example 7 84.91 19.56 18.72 Comparative Example 1 85.04 18.49 18.92 Comparative Example 2 83.21 18.73 18.50 Comparative Example 3 79.87 18.45 18.31 Comparative Example 4 81.01 19.07 18.27 Comparative Example 5 83.07 18.86 18.58

[0137] Table 2 shows that the air permeability of the fabric decreased slightly after finishing, which is because the gaps between the yarns are filled with microcapsules after finishing. The warp strength of the finished fabric increased, while the weft strength remained stable with a slight decrease. The increase in warp breaking elongation is due to the rearrangement of the cellulose microfiber structure inside the cotton fibers after NaOH treatment, resulting in fewer crystalline regions, more amorphous regions, larger fiber cross-sectional area, slightly shorter length, reduced yarn tension, and increased inter-fiber bonding, making the yarn more compact. The change in weft breaking elongation is because the weft yarn experiences less tension during weaving and originally has higher extensibility. After NaOH treatment, fiber expansion and microfiber structure rearrangement may lead to a decrease in fiber extensibility. Therefore, it is necessary to control the fabric pretreatment time to maintain its mechanical properties.

[0138] Table 3. Test results for Escherichia coli ATCC8739

[0139]

[0140] Table 4. Test results for Escherichia coli ATCC8739

[0141]

[0142] Table 5. Results of Staphylococcus aureus ATCC 6538 test.

[0143]

[0144] Table 6. Results of Staphylococcus aureus ATCC 6538 test.

[0145]

[0146] Table 7. Test results of Candida albicans ATCC10231

[0147]

[0148] Table 8. Test results of Candida albicans ATCC10231

[0149]

[0150] As shown in Tables 3-8, the fabrics with added probiotics exhibit good antibacterial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans. The initial antibacterial rate is lower than that after extended storage time because the probiotics enter a dormant state during the reaction. With time, the probiotics regain activity due to the addition of prebiotics to the microcapsules, thus increasing the antibacterial rate. Examples 3 and 4, due to reduced wall material cross-linking, are more prone to breakage under external force, resulting in slightly higher antibacterial rates than Examples 1 and 2. Comparative Example 2 has the highest initial antibacterial rate because chitosan is a good antibacterial agent, with better antibacterial effects than PHA and PLGA. Simultaneously, with increasing storage time, the probiotics completely colonize and proliferate on the fabric, thus the antibacterial rate of the example samples gradually increases. In contrast, the fabric impregnated with pure bacterial solution in Comparative Example 5, lacking protection and energy-supplying substances for the probiotics, experiences a decrease in the number of viable bacteria over time, resulting in a gradual decrease in its antibacterial rate.

[0151] Table 9 Results of Enzyme-Linked Immunosorbent Assay

[0152]

[0153] Table 10 Results of Enzyme-Linked Immunosorbent Assay

[0154]

[0155] As can be seen from Tables 9 and 10, the adsorption rate generally increases slightly with the increase of storage time. Among them, the adsorption rate of fabrics treated with single-walled microcapsules first increases and then decreases. This is because single-walled microcapsules are more easily disintegrated than double-walled ones. Therefore, after the microcapsules disintegrate, probiotics first colonize the fabric surface and can decompose allergens. As time goes by, due to the protection and energy supply of the wall material and prebiotics, the probiotics gradually die, so the adsorption rate decreases.

[0156] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Anyone skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be determined by the claims.

Claims

1. A method for preparing antibacterial and anti-allergic fabrics, characterized in that, Includes the following steps: Unsized cotton fabric is placed in an alkaline solution, boiled, degreased and impurities are removed, and surface hydroxyl groups are introduced to obtain pretreated cotton fabric. Antibacterial and anti-allergic probiotic microcapsules were dispersed in a cationic solution, and a cross-linking agent was added to obtain a cross-linking agent solution; The pretreated cotton fabric is placed in a cross-linking agent solution for cross-linking; after the cross-linking is completed, it is washed and dried to obtain an antibacterial and anti-allergic fabric. The preparation method of the antibacterial and anti-allergic probiotic microcapsules is as follows: (1) The activated probiotic solution was washed and resuspended to obtain a probiotic resuspension; wherein the probiotic was Bacillus subtilis; (2) Add inulin or fucoidan to the probiotic suspension and mix evenly. Then add the sub-outer wall material solution and mix well to form an aqueous phase. Add the oil phase to the aqueous phase, stir, add the crosslinking agent, stir, centrifuge, and wash to obtain single-wall microcapsules. The sub-outer wall material is one of sodium alginate or sodium alginate-chitosan. (3) Disperse the single-walled microcapsules in water, add the outer wall material solution, evaporate by rotary evaporation, centrifuge, wash and freeze dry to obtain double-walled microcapsules; wherein the outer wall material is one or two of polyhydroxy fatty acid ester and polylactic acid-hydroxyacetic acid copolymer.

2. The method according to claim 1, characterized in that, In step (2), the volume ratio of the aqueous phase, oil phase, and crosslinking agent is 1:2:1 to 1:3:

1.

3. The method according to claim 1, characterized in that, In step (2), the crosslinking agent is an aqueous solution of CaCl2 with a concentration of 0.05-0.5M.

4. The method according to claim 1, characterized in that, The alkaline solution is a NaOH aqueous solution with a mass concentration of 1-3%.

5. The method according to claim 1, characterized in that, The cationic solution is an aqueous solution of polydiallyldimethylammonium chloride with a mass fraction of 0.1-0.5 wt%.

6. The method according to claim 1, characterized in that, The crosslinking agent is citric acid, and the mass fraction of the crosslinking agent in the cationic solution is 0.5-1%.

7. The antibacterial and anti-allergic fabric prepared by the method according to any one of claims 1-6.

8. A method for improving the antibacterial and anti-allergic properties of cotton fabrics, characterized in that, The antibacterial and anti-allergic fabric described in claim 7 is used.

9. A type of clothing fabric, characterized in that, The antibacterial and anti-allergic fabric described in claim 7 is used.

10. The application of the antibacterial and hypoallergenic fabric of claim 7 in clothing textiles.

Images