A device for diluting and concentrating microbial samples

Abstract

The present invention provides a microbial sample dilution concentration device, comprising a base, a shaking mechanism, a turbidimeter, a test tube and a dripping mechanism. The shaking mechanism is fixed on the base, and the turntable and the rotating column driven by the No. 1 motor drive the test tube to shake at multiple angles to ensure that the sample is evenly distributed in the test tube. The dripping mechanism includes a No. 2 motor, a screw and a slider. The screw drives the slider to push the syringe to accurately inject a certain amount of sterile water into the test tube to achieve sample dilution. The device can be connected to an external controller, and the user can input the target concentration. The system automatically calculates the amount of sterile water required for dilution according to the initial concentration displayed by the turbidimeter, and accurately controls the number of rotations of the No. 2 motor to inject water; at the same time, the No. 1 motor drives the test tube to be shaken to ensure that the sample is evenly distributed. The entire process is automated, which improves the sample preparation efficiency and measurement accuracy, saving time and effort.

Description

Technical Field

[0001] The invention relates to the field of medical testing equipment, in particular to a microbial sample dilution concentration device. Background Art

[0002] In microbiological research and experiments, in order to meet precise research requirements, such as microbial counting, drug sensitivity testing, and bacterial species identification, it is necessary to accurately dilute microbial samples to obtain samples of appropriate concentrations;

[0003] The concentration of existing microbial samples in the laboratory is measured by turbidimeter and then diluted manually. This operation can only increase the sample concentration but not decrease it without adding additional solution. To achieve the target concentration, the operator needs to manually add samples in small amounts multiple times. The operation is relatively cumbersome and prone to exogenous contamination. It requires certain work experience for the operator and is time-consuming and laborious when the workload is large. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides the following technical solutions:

[0005] A microbial sample dilution concentration device comprises a base, a shaking mechanism and a dripping mechanism; the shaking mechanism is fixedly mounted on the base, the shaking mechanism comprises a bottom plate and a square frame, a No. 1 motor is fixedly mounted on the bottom plate, a turntable is fixedly connected to the output shaft of the No. 1 motor, a rotating column is rotatably mounted on the turntable, a connecting seat is fixedly mounted on the rotating column, the square frame is fixedly mounted on the connecting seat, and a slide plate is slidably mounted in the square frame;

[0006] The dripping mechanism includes a support seat, a screw rod, and a slider. Three baffles are fixedly provided on the support seat. A guide rod is fixedly installed inside the support seat. A No. 2 motor is fixedly installed on one side of the support seat. The screw rod is fixedly installed on the output shaft of the No. 2 motor. The slider is movably installed on the support seat. The slider is threadedly connected to the screw rod, and the guide rod passes through the slider.

[0007] Furthermore, the upper surface of the turntable is an inclined surface, and the rotating column is installed at the higher end of the inclined surface, so that the rotating column is inclined at a certain angle.

[0008] Furthermore, a spring is fixedly installed at the rear of the slide plate, and the spring is fixedly connected to the frame. A plurality of snap-fit ​​buckles are fixedly installed on the slide plate, and a plurality of card interfaces are provided on the frame corresponding to the snap-fit ​​buckles.

[0009] Furthermore, a turbidimeter is fixedly mounted on the slide.

[0010] Furthermore, a support plate is fixedly mounted on the base, and the support seat is fixedly connected to the base via the support plate.

[0011] Furthermore, a U-shaped slide groove is provided on the slide block, and a groove is provided on the front baffle plate of the support seat.

[0012] Furthermore, a stud is fixedly installed on the front baffle of the support seat, a pressure plate is movably installed on the stud, a bolt is movably installed on the pressure plate, and the bolt and the stud are matched through threads.

[0013] Furthermore, the dripping mechanism also includes a syringe, the front end of which is fixed in the groove of the front baffle of the support seat through a pressure plate, and the rear end of the syringe is installed in the U-shaped groove of the slider.

[0014] Compared with the prior art, the technical solution of this application has the following beneficial effects:

[0015] A microbial sample dilution concentration device of the present invention has a No. 2 motor fixedly installed in a support seat, an output shaft of the No. 2 motor fixedly connected to a screw rod, a slider being threadedly connected to the screw rod, a rear end of a syringe being installed in a U-shaped groove of the slider, and a front end of the syringe being fixed to the support seat through a pressing plate; the No. 2 motor is controlled to drive the screw rod to rotate, so that the slider can be driven to move forward, and the slider pushes the syringe push rod to move forward the same distance, so that sterile water is injected into a test tube to automatically dilute the microbial sample;

[0016] A turntable is fixedly connected to the output shaft of motor No. 1, a rotating column is rotatably installed on the turntable, a connecting seat is fixedly installed on the rotating column, a square frame is fixedly installed on the connecting seat, a slider is slidably installed in the square frame, a turbidimeter is fixedly installed on the slider, and a test tube is placed in the turbidimeter; when the dripping mechanism adds a proper amount of sterile water into the test tube, the turntable is driven to rotate by the rotation of motor No. 1, the turntable drives the rotating column to rotate, and the rotating column drives the square frame to rotate, so that the test tube in the turbidimeter can be shaken, thereby ensuring that the microbial sample in the test tube is evenly distributed and the measurement is accurate; the whole process can be achieved by controlling the rotation of the motor, can be precisely controlled, does not require manual intervention, does not require the experimental operator to have certain work experience requirements, saves time and effort. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the appearance of the present invention;

[0018] Figure 2 It is a schematic diagram of the shaking mechanism of the present invention;

[0019] Figure 3 It is a partial top view of the present invention;

[0020] Figure 4 It is a schematic diagram of the instillation mechanism of the present invention;

[0021] In the figure: base 1, support plate 2, shaking mechanism 3, bottom plate 301, motor No. 1 302, turntable 303, rotating column 304, connecting seat 305, frame 306, slide plate 307, snap buckle 308, spring 309, turbidimeter 4, test tube 5, dripping mechanism 6, support seat 601, guide rod 602, motor No. 2 603, screw rod 604, slider 605, stud 606, bolt 607, pressure plate 608, syringe 7. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Embodiment 1:

[0024] See also Figure 1-4 A device for diluting and concentrating a microbial sample comprises a base 1, a shaking mechanism 3, a turbidimeter 4 and a test tube 5; the shaking mechanism 3 is fixedly mounted on the base 1, and the shaking mechanism 3 comprises a bottom plate 301 and a frame 306, a No. 1 motor 302 is fixedly mounted on the bottom plate 301, a turntable 303 is fixedly connected to the output shaft of the No. 1 motor 302, the upper surface of the turntable 303 is an inclined surface, a rotating column 304 is rotatably mounted on the turntable 303, and the rotating column 304 is mounted at the higher end of the inclined surface; a connecting seat 305 is fixedly mounted on the rotating column 304, and the frame 306 is fixedly mounted on the connecting seat 305, and a slide plate 307 is slidably mounted in the frame 306; a spring 309 is fixedly mounted on the back of the slide plate 307, and the spring 309 is fixedly connected to the frame 306; a plurality of snap-fit ​​buckles 308 are fixedly mounted on the slide plate 307, and a plurality of card interfaces are correspondingly provided on the frame 306; the slide plate 307 is fixed in the frame 306 by the snap-fit ​​buckles 308, and when the snap-fit ​​buckles 308 are unlocked, the slide plate 307 will pop out under the action of elastic force; the turbidimeter 4 is fixedly mounted on the slider 605, and the test tube 5 is placed in the turbidimeter 4;

[0025] The turntable 303 is driven to rotate by the rotation of the No. 1 motor 302, and the turntable 303 drives the rotating column 304 to rotate, and the rotating column 304 drives the square frame 306 to rotate, so that the four sides of the square frame 306 will fluctuate and shake around the center; in this way, the microbial sample in the test tube 5 can be evenly distributed in the test tube 5, and the measurement result can be accurate.

[0026] Embodiment 2:

[0027] A device for diluting and concentrating a microbial sample, further comprising a support plate 2, a dripping mechanism 6 and a syringe 7; the support plate 2 is fixedly mounted on a base 1; the dripping mechanism 6 comprises a support seat 601, a screw rod 604 and a slider 605; the support seat 601 is fixedly connected to the base 1 via the support plate 2 and the support seat 601 is vertically mounted, three baffles are fixedly arranged on the support seat 601, two guide rods 602 are fixedly mounted in the support seat 601, a No. 2 motor 603 is fixedly mounted in the two baffles at the rear end of the support seat 601, the screw rod 604 is fixedly mounted on the output shaft of the No. 2 motor 603, and the other end of the screw rod 604 is rotatably mounted in the front baffle of the support seat 601 ; The slider 605 is movably installed in the two front baffles of the support seat 601, the slider 605 is threadedly connected to the screw rod 604, and the guide rod 602 passes through the slider 605; a U-shaped slide groove is provided on the slider 605, and a groove is provided on the front baffle of the support seat 601; a stud 606 is fixedly installed on the front baffle of the support seat 601, a pressure plate 608 is movably installed on the stud 606, and a bolt 607 is movably installed on the pressure plate 608, and the bolt 607 is threadedly matched with the stud 606; the front end of the syringe 7 is fixed in the groove of the front baffle of the support seat 601 through the pressure plate 608, and the rear end of the syringe 7 is installed in the U-shaped groove of the slider 605, and is located above the test tube 5;

[0028] When the syringe 7 is filled with sterile water, its front end is placed in the groove of the front baffle of the support seat 601, and the end of the push rod of the syringe 7 is placed in the U-shaped groove of the slider 605. The syringe 7 is then fixed with a pressure plate 608, and then the pressure plate 608 is fixed with a bolt 607; at this time, by controlling the No. 2 motor 603 to drive the screw rod 604 to rotate, the slider 605 can be driven to move forward, and the slider 605 pushes the push rod of the syringe 7 to move forward the same distance, and sterile water is injected into the test tube 5 to dilute the microbial sample.

[0029] Embodiment 3:

[0030] A microbial sample dilution concentration device of the present invention can dilute the sample by controlling the second motor 603 to add sterile water to the test tube 5; by controlling the number of revolutions of the motor and the pitch of the screw 604, the distance that the slider 605 moves forward after the motor rotates one circle can be obtained, and the volume of the sterile water added can be calculated in combination with the diameter of the syringe 7; thus, the amount of sterile water added can be controlled by controlling the number of revolutions of the motor; after the sterile water is added, the microbial sample in the test tube 5 can be evenly distributed in the test tube 5 by the action of the shaking mechanism 3, so as to ensure the accuracy of the measurement result; when the device is used, it can be controlled in real time by an external controller, the user can input the required concentration, and then read the initial concentration displayed by the turbidimeter 4, and then calculate the amount of sterile water needed to be added to reach the target concentration, and then the second motor 603 can be controlled to rotate the corresponding number of revolutions, the required sterile water is added, and then the first motor 302 is controlled to drive the test tube 5 to shake, so that the microbial sample is evenly distributed; then the concentration is measured and read to ensure the accuracy of the result, and the whole process can be completed automatically by the device without human intervention.

[0031] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A device for diluting and concentrating a microbial sample, characterized in that: It includes a base, a shaking mechanism and a dripping mechanism; the shaking mechanism is fixedly installed on the base, the shaking mechanism includes a bottom plate and a square frame, a No. 1 motor is fixedly installed on the bottom plate, a turntable is fixedly connected to the output shaft of the No. 1 motor, a rotating column is rotatably installed on the turntable, a connecting seat is fixedly installed on the rotating column, the frame is fixedly installed on the connecting seat, and a slide plate is slidably installed in the square frame; The dripping mechanism includes a support seat, a screw rod, and a slider. Three baffles are fixedly provided on the support seat. A guide rod is fixedly installed inside the support seat. A No. 2 motor is fixedly installed on one side of the support seat. The screw rod is fixedly installed on the output shaft of the No. 2 motor. The slider is movably installed on the support seat. The slider is threadedly connected to the screw rod, and the guide rod passes through the slider.

2. A microbial sample dilution concentration device as claimed in claim 1, characterized in that: The upper surface of the turntable is an inclined surface, and the rotating column is installed at the higher end of the inclined surface.

3. A microbial sample dilution concentration device as claimed in claim 1, characterized in that: A spring is fixedly installed at the rear of the slide plate, and the spring is fixedly connected to the frame. A plurality of snap-fit ​​buckles are fixedly installed on the slide plate, and a plurality of card interfaces are provided on the frame corresponding to the snap-fit ​​buckles.

4. A microbial sample dilution concentration device as claimed in claim 1, characterized in that: A turbidimeter is fixedly mounted on the slide.

5. A microbial sample dilution concentration device as claimed in claim 1, characterized in that: A support plate is fixedly mounted on the base, and the support seat is fixedly connected to the base via the support plate.

6. A microbial sample dilution concentration device as claimed in claim 1, characterized in that: The slide block is provided with a U-shaped slide groove, and the front baffle plate of the support seat is provided with a groove.

7. A microbial sample dilution concentration device as claimed in claim 7, characterized in that: A stud is fixedly mounted on the front baffle of the support seat, a pressure plate is movably mounted on the stud, a bolt is movably mounted on the pressure plate, and the bolt and the stud are matched through threads.

8. A microbial sample dilution concentration device as claimed in claim 8, characterized in that: The dripping mechanism also includes a syringe, the front end of which is fixed in the groove of the front baffle of the support seat through a pressing plate, and the rear end of the syringe is installed in the U-shaped groove of the slider.

Images