On-orbit microbiological microscopic detection device

Abstract

The invention discloses an on-orbit microbiological microscopic observation device. The device comprises a device body, the chip clamping box is inserted into the device body and is used for performing microorganism killing detection; the observation module is arranged in the device body and is used for observing the growth condition and the killing effect of microorganisms in the chip card box; the control module is arranged in the device body, electrically connected with the chip card box and the observation module and used for controlling the chip card box and the observation module; the chip card box comprises a card box shell and a chip assembly arranged in the card box shell; the chip card box is provided with chip assemblies with different structures so as to meet the culture and killing detection requirements of different microorganisms; according to the device, chip assemblies with different structures are arranged, so that different killing detection and research on different microorganisms are realized, the requirements of killing performance testing of various microorganism samples can be met, and the applicability and the flexibility of the device are improved.

Description

Technical Field

[0001] The present invention relates to the field of biomedical detection, and in particular to an on-orbit microbial microscopic detection device. Background Art

[0002] With the rapid development of aerospace technology, the space station has become an important platform for human exploration of the universe and scientific research. However, as humans and cargo continue to enter the space station, Earth microorganisms will inevitably enter the space environment, bringing unprecedented challenges to the safety and control of microorganisms in the space station. In the manned sealed cabin of the space station, the environment is complex and changeable, and the sources of microorganisms are diverse, including those carried by astronauts themselves, carried by cargo, and transmitted through the air. At present, the space station mainly uses air filtration devices to remove floating microorganisms in the air, and uses vacuum cleaners and wipes soaked in detergents or disinfectants to clean the surfaces of the cabin. In addition, the space station has also formulated a strict cleaning system to ensure the hygiene of the cabin environment. However, there are many shortcomings in the existing disinfection methods. First, the use of consumable materials is huge, such as vacuum cleaner bags, paper towels, water disinfectants, etc. These materials need to be continuously replenished from the ground, which brings great pressure to the supply of materials to the space station. Secondly, the workload of surface cleaning is large, and astronauts need to spend a lot of time and energy to clean the surfaces of the cabin. In addition, it is difficult to reach the microorganisms in the back panel, dead corners and gaps of the equipment using traditional methods, resulting in poor cleaning effect. Furthermore, excessive use of disinfectants can cause microorganisms to develop resistance to them, especially as the space station's operating time increases, more and more resistant microorganisms have appeared in the cabin. After these resistant microorganisms form biofilms, they are more resistant to antimicrobial agents, and simply wiping with disinfectant wipes containing ordinary chemical ingredients cannot completely remove them; and researchers have found that antimicrobial drug molecules become unstable under microgravity and radiation conditions, and the antimicrobial effect will be weakened. At the same time, the existing space station's microbial killing detection device cannot accurately evaluate the bactericidal effect of microorganisms. Summary of the invention

[0003] In order to overcome the defects of the above-mentioned prior art, the present invention provides an on-orbit microbial microscopic observation device, which is used to solve the problem that the existing space station disinfection methods cannot meet the requirements of long-term and broad-spectrum sterilization and the sterilization effect is difficult to accurately evaluate.

[0004] In order to achieve the above technical objectives, the following technical solutions are adopted:

[0005] The embodiment of the present invention provides an on-orbit microorganism microscopic detection device, comprising:

[0006] device body;

[0007] A chip card box, inserted into the device body, for performing microbial killing detection;

[0008] An observation module, disposed in the device body, for observing the growth status and killing effect of microorganisms in the chip cartridge;

[0009] A control module is disposed in the device body and is electrically connected to the chip card box and the observation module respectively, so as to control the chip card box and the observation module;

[0010] The chip card box comprises a card box shell and a chip component arranged in the card box shell; the chip card box is provided with chip components of different structures to meet the requirements of cultivating and killing detection of different microorganisms.

[0011] Optionally, the chip assembly includes a first nutrient solution chamber, a first buffer chamber, a first mixing chamber, and a plurality of first reaction units;

[0012] The liquid outlets of the first nutrient solution chamber and the first buffer chamber are respectively connected to the liquid inlet of the first mixing chamber, and the liquid outlets of the first mixing chamber are respectively connected to the liquid inlets of a plurality of first reaction units; the first mixing chamber is pre-placed with freeze-dried microbial strains.

[0013] Optionally, the chip assembly further includes a first control unit; the first control unit includes a first peristaltic pump, a second peristaltic pump and a diaphragm pump;

[0014] The liquid inlet end of the first peristaltic pump is connected to the liquid outlet end of the first nutrient solution chamber, the liquid inlet end of the second peristaltic pump is connected to the liquid outlet end of the first buffer solution chamber, and the liquid outlet ends of the first peristaltic pump and the second peristaltic pump are respectively connected to the liquid inlet end of the first mixing chamber; the liquid inlet end of the diaphragm pump is connected to the liquid outlet end of the first mixing chamber, and the liquid outlet end of the diaphragm pump is respectively connected to the liquid inlet ends of a plurality of first reaction units;

[0015] The input ends of the first peristaltic pump, the second peristaltic pump and the diaphragm pump are electrically connected to the output ends of the control module respectively.

[0016] Optionally, the first reaction unit includes a first solenoid valve and a first sample reaction chamber; the liquid inlet end of the first solenoid valve is connected to the liquid outlet end of the diaphragm pump, and the liquid outlet end of the first solenoid valve is connected to the liquid inlet end of the first sample reaction chamber; the first sample reaction chamber is pre-installed with a first reaction sample;

[0017] An input end of the first solenoid valve is electrically connected to an output end of the control module.

[0018] Optionally, the chip cartridge further comprises a waste liquid cartridge and a plurality of double self-locking quick connectors;

[0019] The waste liquid cartridge comprises a waste liquid cartridge body and a plurality of waste liquid bags arranged in the waste liquid cartridge body; a plurality of waste liquid inlets are provided at the top of the cartridge housing, and the waste liquid inlets are connected to corresponding waste liquid bags;

[0020] One end of the double self-locking quick connector is connected to the liquid outlet of the first sample reaction chamber, and the other end is inserted into or withdrawn from the waste liquid cartridge through the waste liquid inlet to discharge the waste liquid in the first sample reaction chamber into the waste liquid bag.

[0021] Optionally, the chip assembly includes a first reaction liquid chamber, a second reaction liquid chamber, a second nutrient liquid chamber, a second buffer chamber, a second mixing chamber, a plurality of second reaction units, and a plurality of third reaction units;

[0022] The liquid outlets of the first reaction liquid chamber and the second buffer chamber are respectively connected to the liquid inlets of a plurality of second reaction units; the liquid outlets of the second nutrient liquid chamber and the second buffer chamber are respectively connected to the liquid inlet of the second mixing chamber; the liquid outlet of the second reaction liquid chamber is connected to the liquid inlet of the second mixing chamber, and the liquid outlets of the second mixing chamber are respectively connected to the liquid inlets of a plurality of third reaction units; and freeze-dried microbial strains are pre-placed in the second mixing chamber.

[0023] Optionally, the chip assembly further includes a second control unit;

[0024] The input end of the second control unit is electrically connected to the output end of the control module; the second control unit includes a third peristaltic pump, a fourth peristaltic pump, a fifth peristaltic pump, a sixth peristaltic pump, and a seventh peristaltic pump;

[0025] The liquid inlet end of the third peristaltic pump is connected to the liquid outlet end of the first reaction liquid chamber, and the liquid outlet end of the third peristaltic pump is respectively connected to the liquid inlet ends of the plurality of second reaction units; the liquid inlet end of the fourth peristaltic pump is connected to the liquid outlet end of the second buffer chamber, and the liquid outlet end of the fourth peristaltic pump is respectively connected to the second mixing chamber and the liquid inlet ends of the plurality of second reaction units;

[0026] The liquid inlet end of the fifth peristaltic pump is connected to the liquid outlet end of the second nutrient liquid chamber, the liquid inlet end of the sixth peristaltic pump is connected to the liquid outlet end of the second reaction liquid chamber, and the liquid outlet ends of the fifth peristaltic pump and the sixth peristaltic pump are respectively connected to the liquid inlet end of the second mixing chamber; the liquid inlet end of the seventh peristaltic pump is connected to the liquid outlet end of the second mixing chamber, and the liquid outlet end of the seventh peristaltic pump is respectively connected to the liquid inlet ends of a plurality of third reaction units.

[0027] Optionally, the second reaction unit includes a second solenoid valve and a second sample reaction chamber; the liquid inlet end of the second solenoid valve is connected to the liquid outlet ends of the first reaction liquid chamber and the second buffer chamber respectively, and the liquid outlet end of the second solenoid valve is connected to the liquid inlet end of the second sample reaction chamber; the second sample reaction chamber is pre-installed with a second reaction sample;

[0028] The third reaction unit includes a third solenoid valve and a third sample reaction chamber; the liquid inlet end of the third solenoid valve is connected to the liquid outlet end of the seventh peristaltic pump, and the liquid outlet end of the third solenoid valve is connected to the liquid inlet end of the third sample reaction chamber; the third sample reaction chamber is pre-installed with a third reaction sample.

[0029] The input ends of the second solenoid valve and the third solenoid valve are electrically connected to the output end of the control module respectively.

[0030] Optionally, the observation module includes a motion bearing component, a microscopic imaging component, and a light source component;

[0031] The microscopic imaging component is arranged on the motion component, and the motion component drives the microscopic imaging component to perform linear reciprocating motion, so as to realize observation of different areas in the chip card box;

[0032] The microscopic imaging assembly includes an objective lens, a reflector, a microscope tube and a camera; the objective lens is arranged below the chip card box; a perforation is arranged on the top of the reflector, and a laser inlet is arranged on the side of the reflector; the objective lens is located directly above the perforation, one end of the microscope tube is sealed and connected to the reflector, and the other end of the microscope tube is sealed and connected to the camera lens, so as to capture a microscopic image in the chip card box;

[0033] The light source assembly includes an excitation light source unit, a catalytic light source unit and a laser switching unit;

[0034] The excitation light source unit is arranged at the laser entrance of the reflector to generate excitation light of at least two wavelengths; the laser switching unit is connected to the excitation light source unit to switch excitation light of different wavelengths; the catalytic light source unit is arranged around the objective lens to generate catalytic light of at least two wavelengths.

[0035] Optionally, the control module includes an integrated circuit board, which is disposed in the device body and has at least two positioning pins; the card box housing is provided with at least two guide holes corresponding to the positions of the positioning pins, and the positioning pins are inserted into the corresponding guide holes to fix the chip card box;

[0036] The card box housing is provided with a control interface, the input end of the control interface is electrically connected to the output end of the integrated circuit board, and the output end of the control interface is electrically connected to the input end of the chip assembly;

[0037] The device body is provided with a communication interface and a power supply interface; the communication interface is electrically connected to the control module to realize data interaction with external equipment; the power supply interface is electrically connected to the control module to provide power and information exchange.

[0038] The beneficial effects brought by the embodiments provided by the present invention include:

[0039] The present invention realizes different killing detection and research on different microorganisms by setting chip components with different structures, can meet the killing performance test requirements of various microbial samples, and improves the applicability and flexibility of the device;

[0040] The present invention controls the multi-way pump-valve structure and observation module in the chip card box through an integrated circuit board, realizes programmed control of the liquid path in the chip card box and time-sequential fluorescence imaging, ensures a complete closed loop of the experiment, reduces manual intervention, and effectively improves the efficiency of the experiment and the scientific objectivity of the observation results; at the same time, by setting the number of pumps and valves in the chip card box and controlling the working time and opening and closing sequence of the pumps and valves, it is possible to achieve differentiated cultivation and detection of different microorganisms.

[0041] The present invention can observe and photograph the growth status of microorganisms and the microorganism killing effect in real time through the observation module, and transmit the microorganism photos to the ground through the communication interface, providing real-time data for analyzing the test effect of the device and the microorganism growth process. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0043] Figure 1 A schematic diagram of the internal structure of one side of a microorganism microscopic observation device according to an embodiment of the present specification is shown;

[0044] Figure 2 A schematic diagram of the internal structure of another side of the microorganism microscopic observation device according to an embodiment of the present specification is shown;

[0045] Figure 3 A schematic diagram of the three-dimensional structure of a microorganism microscopic observation device according to an embodiment of the present specification is shown;

[0046] Figure 4 A top view of a microorganism microscopic observation device according to an embodiment of the present specification is shown;

[0047] Figure 5 A schematic diagram of the internal structure of a chip card box according to an embodiment of the present specification is shown;

[0048] Figure 6 A top view of a chip card box according to an embodiment of the present specification is shown;

[0049] Figure 7A schematic structural diagram of a first chip assembly according to an embodiment of this specification is shown;

[0050] Figure 8 shows a perspective view of an observation module according to an embodiment of the present specification;

[0051] Fig. 9 shows a side view of an observation module according to an embodiment of the present specification;

[0052] Fig.10 shows a top view of an observation module according to an embodiment of the present specification;

[0053] Fig.11 A detection flow chart of a first chip assembly according to an embodiment of this specification is shown;

[0054] Fig.12 A schematic structural diagram of a second chip assembly according to an embodiment of this specification is shown;

[0055] Fig.13 Another structural schematic diagram of a second chip assembly according to an embodiment of this specification is shown;

[0056] Fig.14 shows a detection flow chart of a second chip assembly according to an embodiment of this specification;

[0057] Among them, 10 is the device body; 101 is the power supply interface; 102 is the communication interface; 103 is the chip insertion port; 20 is the chip card box assembly; 201 is the box body; 202 is the cover body; 203 is the captive screw; 204 is the control interface, 205 is the handle; 206 is the first peristaltic pump; 207 is the second peristaltic pump; 208 is the diaphragm pump; 209 is the first solenoid valve; 210 is the first sample reaction chamber; 211 is the first control valve; 212 is the second control valve; 213 is the third peristaltic pump; 214 is the fourth peristaltic pump; 215 is the fifth peristaltic pump; 216 is the sixth peristaltic pump; 217 is the seventh peristaltic pump; 218 is the second mixing chamber; 219 is the second sample reaction chamber; 220 is the third sample reaction chamber; 221 is the second solenoid valve ; 222 is the third solenoid valve; 223 is the third control valve; 30 is the observation module; 301 is the motion support rail; 302 is the drive motor; 303 is the sliding platform; 304 is the objective lens; 305 is the reflector; 306 is the microscope tube; 307 is the camera; 308 is the objective lens focusing unit; 309 is the excitation light source unit; 310 is the catalytic light source unit; 311 is the linear motor; 312 is the lead screw; 40 is the integrated circuit board; 401 is the electrical connector; 402 is the positioning pin. DETAILED DESCRIPTION

[0058] In order to make the objectives, technical solutions and advantages of the present disclosure more clearly understood, the present disclosure is further described in detail below in combination with specific embodiments and with reference to the accompanying drawings.

[0059] However, it should be understood that these descriptions are exemplary only and are not intended to limit the scope of the present disclosure. In the following detailed description, for ease of explanation, many specific details are set forth to provide a comprehensive understanding of the embodiments of the present disclosure. However, it is apparent that one or more embodiments may also be implemented without these specific details. In addition, in the following description, the description of the known technology is omitted to avoid unnecessary confusion of the concepts of the present disclosure.

[0060] The terms used herein are only for describing specific embodiments and are not intended to limit the present disclosure. The term "comprising" used herein indicates the existence of features, steps, operations, but does not exclude the existence or addition of one or more other features. It should be noted that all terms (including technical and scientific terms) used herein have the meanings commonly understood by those skilled in the art, unless otherwise defined. It should be noted that the terms used here should be interpreted as having meanings consistent with the context of this specification, and should not be interpreted in an idealized or overly rigid manner.

[0061] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0062] Example 1

[0063] like Figure 1-Figure 10 As shown, this embodiment provides an on-orbit microorganism microscopic detection device, including a device body 10, a chip card box 20, an observation module 30 and a control module;

[0064] Exemplarily, the chip card box 20 is inserted into the device body 10 to perform microbial killing detection; the observation module 30 is arranged in the device body 10 to observe the growth status and killing effect of microorganisms in the chip card box 20; the control module is arranged in the device body 10, and the control module is electrically connected to the chip card box 20 and the observation module 30 respectively, so as to control the chip card box 20 and the observation module 30;

[0065] The chip card box 20 includes a card box shell and a chip component disposed in the card box shell; the chip card box 20 is provided with chip components of different structures to meet the requirements of culturing and killing detection of different microorganisms.

[0066] In this embodiment, the chip component is a first chip component. The first chip component conducts a physical bactericidal performance experiment by setting different pump valve structures, multiple chambers and pre-setting antibacterial samples with different bionic nanostructures to detect the physical bactericidal performance of antibacterial samples with different nanostructures.

[0067] In some embodiments, the first chip assembly includes a first nutrient solution chamber, a first buffer chamber, a first mixing chamber, a first control unit, a first control valve 211, a second control valve 212, and eight first reaction units; wherein the first control unit includes a first peristaltic pump 206, a second peristaltic pump 207, and a diaphragm pump 208;

[0068] Specifically, the liquid outlet of the first nutrient solution chamber is connected to the liquid inlet of the first mixing chamber through the first peristaltic pump 206, the liquid outlet of the first buffer solution chamber is connected to the liquid inlet of the first mixing chamber through the second peristaltic pump 207, and the liquid outlet of the first mixing chamber is connected to the liquid inlet of the first control valve 211 through the diaphragm pump 208; the liquid outlet of the first control valve 211 is respectively connected to the liquid inlet of the eight first reaction units, and the liquid outlets of the eight first reaction chambers are all connected to the liquid inlet of the second control valve 212; the input ends of the first control valve 211, the second control valve 212, the first peristaltic pump 206, the second peristaltic pump 207 and the diaphragm pump 208 are respectively electrically connected to the output end of the control module;

[0069] In this embodiment, phosphate buffer solution (PBS buffer) is stored in the first buffer chamber, and freeze-dried Escherichia coli pellets are pre-installed in the first mixing chamber; the first control valve 211 and the second control valve 212 both use multi-chamber solenoid valves to simultaneously control the fluid flow of multiple chambers; the diaphragm pump 208 uses a pneumatic diaphragm pump 208 to provide a high flow rate to achieve the collision of the liquid in the first mixing chamber with the antibacterial sample.

[0070] In some embodiments, the first reaction unit includes a first solenoid valve 209 and a first sample reaction chamber 210;

[0071] Specifically, the liquid inlet of the first solenoid valve 209 is connected to the liquid outlet of the first control valve 211, the liquid outlet of the first solenoid valve 209 is connected to the liquid inlet of the first sample reaction chamber 210, and the liquid outlet of the first sample reaction chamber 210 is connected to the liquid inlet of the second control valve 212; the input end of the first solenoid valve 209 is electrically connected to the output end of the control module;

[0072] In this embodiment, the first chip assembly includes 8 first sample reaction chambers 210A1-A8 and 8 corresponding first solenoid valves 209, and the first solenoid valves 209 independently control the liquid path opening and closing and liquid path flow rate of the corresponding first sample reaction chambers 210; wherein, the 8 first sample reaction chambers 210 are respectively pre-set with antibacterial samples of different bionic nanostructures; and the bottom ends of the 8 first sample reaction chambers 210 are all provided with waste liquid outlets;

[0073] In some embodiments, the chip card box 20 also includes a waste liquid card box and a plurality of double self-locking quick connectors;

[0074] Specifically, the waste liquid cartridge comprises a waste liquid cartridge body and a plurality of waste liquid bags disposed in the waste liquid cartridge body; a plurality of waste liquid inlets are provided at the top of the cartridge housing, and the waste liquid inlets are connected to corresponding waste liquid bags;

[0075] The double self-locking quick connector includes a first connector and a second connector, both of which are self-locking quick connectors and are interconnected. The first connector is fixedly arranged at the liquid outlet end of the second control valve 212, and the other end is inserted into or withdrawn from the waste liquid cartridge through the waste liquid inlet, so as to discharge the waste liquid in the first sample reaction chamber 210 into the waste liquid bag;

[0076] Among them, the liquid inlet end of the first connector is connected to the liquid outlet end of the second control valve 212, the liquid outlet end of the first connector is connected to the liquid inlet end of the second connector, and the liquid outlet end of the second connector is connected to the liquid inlet end of the waste liquid bag through the waste liquid inlet.

[0077] In this embodiment, after the self-locking connector exits the waste liquid cartridge through the waste liquid inlet, both self-locking connectors form a sealing structure to prevent leakage. The liquid outlet of the second connector is wrapped with a layer of absorbent sponge; the absorbent sponge is used to wipe the liquid outlet of the second connector to absorb any droplets that may flow out;

[0078] Exemplarily, the observation module 30 includes a motion bearing assembly, a microscopic imaging assembly, and a light source assembly;

[0079] In some embodiments, the motion bearing assembly includes a motion support rail 301, a drive motor 302, and a sliding platform 303;

[0080] Specifically, the sliding platform 303 is slidably disposed on the motion support rail 301, and the microscopic imaging component is fixedly disposed on the sliding platform 303; the driving motor 302 is disposed at one end of the motion support rail 301, and is used to drive the sliding platform 303 to drive the microscopic imaging component to perform linear reciprocating motion, so as to realize observation of different areas in the chip cartridge 20.

[0081] In some embodiments, the microscopic imaging assembly includes an objective lens 304, a reflector 305, a microscope tube 306, a camera 307, and an objective lens focusing unit 308;

[0082] Specifically, the reflector 305 includes a reflector and an outer shell, a perforation is provided at the bottom of the outer shell, a laser inlet is provided on the side of the outer shell, and the optical mirror is installed in the outer shell; the objective lens 304 is arranged below the chip card box 20, the perforation is located directly below the objective lens 304, and the objective lens focusing unit 308 is used to adjust the distance between the objective lens 304 and the chip card box 20;

[0083] One end of the microscope tube 306 is sealed and connected to the outer shell, and the other end of the microscope tube 306 is sealed and connected to the camera lens 307. The optical axis of the camera lens 307 is on the same straight line as the optical axis of the microscope tube 306. The optical axis of the microscope tube 306 is bent 90 degrees after being refracted by the reflective mirror and is on the same straight line as the optical axis of the objective lens 304, so as to capture the microscopic image in the chip card box 20.

[0084] In some embodiments, the light source assembly includes an excitation light source unit 309, a catalytic light source unit 310, and a laser switching unit;

[0085] Specifically, the excitation light source unit 309 includes at least two excitation light sources with different wavelengths, wherein the excitation light source is arranged at the laser entrance of the reflector 305 to excite the substance in the chip card box 20 to emit fluorescence, so as to realize the fluorescence microscopic observation of the microorganisms; the laser switching unit includes a linear motor 311 and a lead screw 312, one end of the lead screw 312 is connected to the linear motor 311, and the other end is connected to at least two excitation light sources, so as to drive the excitation light source to move through the linear motor 311 to realize the switching of different excitation lights; the catalytic light source unit 310 includes at least two catalytic light sources with different wavelengths, which are arranged around the objective lens 304 to generate catalytic light to catalyze the microbial reaction process in the chip card box 20 and complete the catalytic reaction of multiple microorganisms.

[0086] Among them, the driving motor 302, the camera 307, the focusing unit of the objective lens 304, the excitation light source unit 309, the catalytic light source unit 310 and the laser switching unit are all electrically connected to the control module.

[0087] In this embodiment, the microscopic imaging component can take pictures of the reaction chambers in the chip card box 20 one by one, or take pictures of a specific reaction chamber according to experimental needs; the laser switching unit can autonomously switch the fluorescence excitation light source according to experimental needs.

[0088] In some embodiments, the control module includes an integrated circuit board 40; the integrated circuit board 40 is disposed in the device body 10, and at least two positioning pins 402 are provided on the integrated circuit board 40; at least two guide holes corresponding to the positions of the positioning pin holes are opened on the card box housing, and the positioning pins 402 are inserted into the corresponding guide holes to fix the chip card box 20;

[0089] At least two positioning pins are provided on the integrated circuit board; at least two guide holes corresponding to the positions of the positioning pins are provided on the card box housing, and the positioning pins are inserted into the corresponding guide holes to fix the chip card box;

[0090] In some embodiments, a control interface 204 is disposed on the cartridge housing, and an electrical connector 401 is disposed on the integrated circuit board 40 . The electrical connector 401 is plugged into the control interface 204 to control a plurality of pumps and valve structures within the chip assembly.

[0091] In some embodiments, the card box housing is a hollow rectangular cube, which is composed of a box body 201 and a cover body 202. The cover body 202 also includes a groove arranged on the top wall of the cover body 202. The groove is used to place a sealing ring, so that when the box body 201 and the cover body 202 are covered, the opening of the box body 201 contacts the sealing ring, which plays a role of fixing and environmental sealing;

[0092] In this embodiment, a chip insertion port 103 is provided at the top of the device body 10 for inserting the chip card box 20; wherein the device body 10 also includes a protective cover for covering the chip insertion port 103 to prevent dust, liquid or other contaminants from entering the chip insertion port 103, thereby protecting the cleanliness and safety of the chip card box.

[0093] In order to ensure the stability of the chip card box 20, the device body 10 and the chip card box 20 are locked by at least two captive screws 203; when working, the captive screws 203 can be manually unscrewed to quickly complete the insertion and removal operation of the chip card box 20; wherein, a handle 205 is provided on the chip card box 20 to facilitate the insertion and removal of the chip card box 20.

[0094] In some embodiments, a communication interface 102 and a power supply interface 101 are provided on the device body 10; the communication interface 102 is electrically connected to the control module to realize data interaction with external devices; the power supply interface 101 is electrically connected to the control module to provide power.

[0095] In some embodiments, the liquid circuits in the chip card box 20 are all connected by hoses, wherein the hose material is PE, which is non-toxic and odorless, has a melting point of 100-130°C, has excellent low-temperature resistance, can still maintain good mechanical properties at -60°C, is insoluble in any known solvent at room temperature, and does not produce biological toxicity.

[0096] like Fig.11As shown, this embodiment includes a physical sterilization experiment. The physical sterilization experiment is as follows: first, the nutrient solution in the nutrient solution chamber is pumped into the first mixing chamber to fully mix the nutrient solution and the Escherichia coli strain freeze-dried balls; the strains in the first mixing chamber are incubated and cultured at room temperature. After the culture is completed, the PBS buffer in the buffer chamber is pumped into the first mixing chamber, fully mixed, and a bacterial suspension with a standard concentration of 5×103~5×104 CFU / mL is prepared. 0.1 mL of the bacterial suspension is respectively injected into 8 first sample reaction chambers 210A1-A8. The high flow rate provided by the peristaltic pump is used to make the bacterial suspension collide with the surfaces of 8 groups of bionic nano-antibacterial samples with different structures, and contact for 10 minutes to perform physical sterilization. After the sterilization is completed, the surface bacteria are eluted with 0.5 mL of PBS buffer, and the eluted waste liquid is collected through a waste liquid cartridge and stored at 4°C. After low-temperature storage, it is descended with the spacecraft and further experiments are performed on the ground.

[0097] It should be noted that, for the microbial microscopic detection device provided in this embodiment, the user can independently design different chambers, pump and valve structures and preset various samples according to the requirements of different microbial killing experiments to construct different chip components in the chip card box 20, control the different chip components through the control module, and use the observation module 30 to perform microscopic observation and fluorescence observation.

[0098] Example 2

[0099] like Figure 12-13 As shown, this embodiment provides an on-orbit microorganism microscopic detection device, which is different from the embodiment 1 in that the chip assembly is a second chip assembly;

[0100] In this embodiment, the second chip component conducts a photodynamic-physical coupled bactericidal performance experiment by setting different pump valve structures, multiple chambers, different probe solutions, and antibacterial samples with preset different nanostructures to detect the bactericidal performance of antibacterial samples sprayed with different probe solutions under different lighting conditions.

[0101] Exemplarily, the second chip assembly includes a first reaction liquid chamber, a second reaction liquid chamber, a second nutrient liquid chamber, a second buffer chamber, a second mixing chamber 218, a third control valve, three second reaction units, three third reaction units, and six waste liquid chambers;

[0102] Specifically, the second control unit includes a third peristaltic pump 213, a fourth peristaltic pump 214, a fifth peristaltic pump 215, a sixth peristaltic pump 216, and a seventh peristaltic pump 217; the input ends of the third peristaltic pump 213, the fourth peristaltic pump 214, the fifth peristaltic pump 215, the sixth peristaltic pump 216, and the seventh peristaltic pump 217 are electrically connected to the output ends of the control module, respectively.

[0103] In this embodiment, the second buffer chamber contains phosphate buffer (PBS buffer), and the second mixing chamber is pre-placed with freeze-dried Escherichia coli bacteria pellets;

[0104] In some embodiments, the liquid outlet of the first reaction liquid chamber is connected to the liquid inlet of the three second reaction units through the third peristaltic pump 213; the liquid outlet of the second buffer liquid chamber is connected to the liquid inlet of the three second reaction units through the fourth peristaltic pump 214;

[0105] Specifically, the second reaction unit includes a second solenoid valve 221 and a second sample reaction chamber 219; the liquid inlet end of the second solenoid valve 221 is connected to the liquid outlet end of the first reaction liquid chamber and the second buffer chamber respectively, and the liquid outlet end of the second solenoid valve 221 is connected to the liquid inlet end of the second sample reaction chamber 219; wherein the input ends of the three second solenoid valves 221 are all electrically connected to the output end of the control module;

[0106] In this embodiment, the second chip assembly includes three second sample reaction chambers 219B1-B3 and three corresponding second solenoid valves 221, and the second solenoid valves 221 independently control the liquid path opening and closing and liquid path flow rate of the corresponding second sample reaction chamber 219; the second sample reaction chambers 219B1, B2, and B3 are all pre-installed with bionic nano-antibacterial samples that retain bacterial remains after sterilization; wherein, the first reaction liquid chamber stores the AIE probe 2 solution, which is a short-wavelength fluorescent probe that can only enter dead bacteria.

[0107] In some embodiments, the liquid outlet of the second buffer chamber is connected to the liquid inlet of the second mixing chamber 218 through the fourth peristaltic pump 214; the liquid outlet of the second nutrient liquid chamber is connected to the liquid inlet of the second mixing chamber 218 through the fifth peristaltic pump 215; the liquid outlet of the second reaction liquid chamber is connected to the liquid inlet of the second mixing chamber 218 through the sixth peristaltic pump 216; the liquid outlet of the second mixing chamber 218 is connected to the liquid inlets of the three third reaction units respectively through the seventh peristaltic pump 217;

[0108] Specifically, the third reaction unit includes a third solenoid valve 222 and a third sample reaction chamber 220; the liquid inlet end of the third solenoid valve 222 is connected to the liquid outlet end of the seventh peristaltic pump 217, and the liquid outlet end of the third solenoid valve 222 is connected to the liquid inlet end of the third sample reaction chamber 220; wherein the input ends of the three third solenoid valves 222 are all electrically connected to the output end of the control module;

[0109] In this embodiment, the second chip assembly includes three third sample reaction chambers 220B4-B6 and three corresponding third solenoid valves 222, and the third solenoid valves 222 independently control the liquid path opening and closing and liquid path flow rate of the corresponding third sample reaction chamber 220; the third sample reaction chambers 220B4, B5, and B6 are all pre-installed with bionic nano-antibacterial samples, and the third sample reaction chamber 220B5 is pre-installed with AIE photosensitizer powder; wherein, the first reaction liquid chamber stores two AIE probes with different functions to form an AIE probe 1 solution, and the AIE probe 1 solution includes a long-wavelength emitting fluorescent probe that can enter live bacteria and dead bacteria and a short-wavelength fluorescent probe that can only enter dead bacteria.

[0110] like Fig.14 As shown, this embodiment includes two groups of sterilization detection experiments: self-cleaning sterilization detection experiment and comprehensive sterilization detection experiment;

[0111] Self-cleaning detection experiment: first apply visible light to the second sample reaction chambers 219B2 and B3, then inject the AIE probe 2 solution into the second sample reaction chambers 219B1-B3 for incubation, and perform fluorescence imaging after a period of time to determine the mortality rate of Escherichia coli; then use PBS buffer to elute the surface bacteria, and store the waste liquid after elution through the waste liquid chamber.

[0112] Comprehensive sterilization test experiment: first, the nutrient solution in the nutrient solution chamber is pumped into the second mixing chamber 218 to fully mix the nutrient solution and the freeze-dried pellets of E. coli bacteria; the bacteria in the second mixing chamber 218 are incubated and cultured at room temperature. After the culture is completed, the PBS buffer in the buffer chamber is pumped into the second mixing chamber 218 by the second peristaltic pump 207, and fully mixed to make a standard concentration of 5×103~5×104 CFU / mL bacterial suspension, pump the AIE probe 1 solution into the second mixing chamber 218 for incubation (the activity of microorganisms can be judged by observing the long-wavelength fluorescence of the second mixing chamber 218, and the death of sterile bacteria can be confirmed by short-wavelength fluorescence); the incubated solution is then injected into the third sample reaction chamber 220B4, B5 and B6 respectively, and visible light is applied to the second sample reaction chamber 219B4 and B5, and the third sample reaction chamber 220B4, B5 and B6 are respectively subjected to photodynamic-physical coupling sterilization test, chemical sterilization test and physical sterilization test. After the reaction in the third sample reaction chamber 220B4, B5 and B6 is completed, fluorescence imaging is performed to determine the survival rate of Escherichia coli. Finally, the surface bacteria are eluted with PBS buffer, and the eluted waste liquid is stored in the waste liquid chamber. Among them, the chemical substance in the chemical sterilization test is the AIE photosensitizer powder preset in advance.

[0113] In each embodiment, when the corresponding experiment is to be carried out, the astronaut first inserts the corresponding chip card box 20 into the device body 10, and the device receives the telemetry signal of the air-ground command, triggers the operation of the integrated circuit board 40, and the integrated circuit board 40 issues instructions to control the different pump valves in the chip card box 20. Through the timing control of the pump valve, the solution in the chip card box 20 is pumped into each chamber to start the on-orbit cultivation of microorganisms. After a period of cultivation, the integrated circuit board 40 turns on the observation module 30, autonomously turns on the corresponding excitation light source, and starts the camera 307. The microscopic imaging component is driven to move by the motion bearing component to observe the changes in the growth of microorganisms in the mixing chamber at the bottom of the card box chip from bottom to top, and take pictures for storage. After the shooting is completed, the excitation light source is turned off, and the corresponding catalytic light source selected autonomously is turned on to catalyze the microorganisms in each reaction chamber to accelerate the shedding of the microorganisms, so as to achieve the acceleration of the self-cleaning of the reaction pool. After the experiment is completed, the image data of the experimental process is transmitted to the ground through the communication interface 102 for analysis and evaluation of the experimental effect by ground researchers.

[0114] In summary, this embodiment realizes different killing detection and research on different microorganisms by setting chip components of different structures, which can meet the killing performance test requirements of various microbial samples and improve the applicability and flexibility of the device;

[0115] The installation method of the chip card box 20 of this embodiment is compatible with the culture analysis of various microorganisms. As long as the card box positioning method and the electrical signal connection type are the same, the device can be used for culture observation, which enhances the versatility of the device.

[0116] In this embodiment, the integrated circuit board 40 controls the multi-way pump valve structure and the observation module 30 in the chip cartridge 20, thereby realizing programmed control and time-sequential fluorescence imaging of the liquid path in the chip cartridge 20, ensuring a complete closed loop of the experiment, reducing manual intervention, and effectively improving the efficiency of the experiment and the scientific objectivity of the observation results;

[0117] This embodiment can realize the observation of differential cultivation and sterilization of different bacterial microorganisms by setting the number of pumps and valves in the chip cartridge 20 and controlling the working time and opening and closing sequence of the pumps and valves. This embodiment is not only suitable for the observation of the growth and cultivation of microorganisms on orbit, but also for the observation of microorganism cultivation for other functional requirements on the ground, providing a self-operating and rapid experimental results monitoring method for the growth of multiple microbial communities for microbial cultivation experiments.

[0118] This embodiment can observe and photograph the growth of microorganisms in real time through the observation module 30, and transmit the microorganism images to the ground through the communication interface 102, providing real-time data for analyzing the test results of the device and the growth process of microorganisms.

[0119] The above description is only a preferred embodiment of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention.

Claims

1. An on-orbit microbial microscopic detection device, characterized in that: include: device body; A chip card box, inserted into the device body, for performing microbial killing detection; An observation module, disposed in the device body, for observing the growth status and killing effect of microorganisms in the chip cartridge; A control module is disposed in the device body and is electrically connected to the chip card box and the observation module respectively, so as to control the chip card box and the observation module; The chip card box comprises a card box shell and a chip component arranged in the card box shell; the chip card box is provided with chip components of different structures to meet the requirements of cultivating and killing detection of different microorganisms.

2. The device according to claim 1, characterized in that The chip assembly includes a first nutrient solution chamber, a first buffer chamber, a first mixing chamber, and a plurality of first reaction units; The liquid outlets of the first nutrient solution chamber and the first buffer chamber are respectively connected to the liquid inlet of the first mixing chamber, and the liquid outlets of the first mixing chamber are respectively connected to the liquid inlets of a plurality of first reaction units; the first mixing chamber is pre-placed with freeze-dried microbial strains.

3. The device according to claim 2, characterized in that The chip assembly further comprises a first control unit; the first control unit comprises a first peristaltic pump, a second peristaltic pump and a diaphragm pump; The liquid inlet end of the first peristaltic pump is connected to the liquid outlet end of the first nutrient solution chamber, the liquid inlet end of the second peristaltic pump is connected to the liquid outlet end of the first buffer solution chamber, and the liquid outlet ends of the first peristaltic pump and the second peristaltic pump are respectively connected to the liquid inlet end of the first mixing chamber; the liquid inlet end of the diaphragm pump is connected to the liquid outlet end of the first mixing chamber, and the liquid outlet end of the diaphragm pump is respectively connected to the liquid inlet ends of a plurality of first reaction units; The input ends of the first peristaltic pump, the second peristaltic pump and the diaphragm pump are electrically connected to the output ends of the control module respectively.

4. The device according to claim 3, characterized in that The first reaction unit comprises a first solenoid valve and a first sample reaction chamber; the liquid inlet end of the first solenoid valve is connected to the liquid outlet end of the diaphragm pump, and the liquid outlet end of the first solenoid valve is connected to the liquid inlet end of the first sample reaction chamber; the first sample reaction chamber is pre-placed with a first reaction sample; An input end of the first solenoid valve is electrically connected to an output end of the control module.

5. The device according to claim 4, characterized in that The chip card box also includes a waste liquid card box and a plurality of double self-locking quick connectors; The waste liquid cartridge comprises a waste liquid cartridge body and a plurality of waste liquid bags arranged in the waste liquid cartridge body; a plurality of waste liquid inlets are provided at the top of the cartridge housing, and the waste liquid inlets are connected to corresponding waste liquid bags; One end of the double self-locking quick connector is connected to the liquid outlet of the first sample reaction chamber, and the other end is inserted into or withdrawn from the waste liquid cartridge through the waste liquid inlet to discharge the waste liquid in the first sample reaction chamber into the waste liquid bag.

6. The device according to claim 1, characterized in that The chip assembly includes a first reaction liquid chamber, a second reaction liquid chamber, a second nutrient liquid chamber, a second buffer chamber, a second mixing chamber, a plurality of second reaction units, and a plurality of third reaction units; The liquid outlets of the first reaction liquid chamber and the second buffer chamber are respectively connected to the liquid inlets of a plurality of second reaction units; the liquid outlets of the second nutrient liquid chamber and the second buffer chamber are respectively connected to the liquid inlet of the second mixing chamber; the liquid outlet of the second reaction liquid chamber is connected to the liquid inlet of the second mixing chamber, and the liquid outlets of the second mixing chamber are respectively connected to the liquid inlets of a plurality of third reaction units; and freeze-dried microbial strains are pre-placed in the second mixing chamber.

7. The device according to claim 6, characterized in that The chip assembly also includes a second control unit; The input end of the second control unit is electrically connected to the output end of the control module; the second control unit includes a third peristaltic pump, a fourth peristaltic pump, a fifth peristaltic pump, a sixth peristaltic pump, and a seventh peristaltic pump; The liquid inlet end of the third peristaltic pump is connected to the liquid outlet end of the first reaction liquid chamber, and the liquid outlet end of the third peristaltic pump is respectively connected to the liquid inlet ends of the plurality of second reaction units; the liquid inlet end of the fourth peristaltic pump is connected to the liquid outlet end of the second buffer chamber, and the liquid outlet end of the fourth peristaltic pump is respectively connected to the second mixing chamber and the liquid inlet ends of the plurality of second reaction units; The liquid inlet end of the fifth peristaltic pump is connected to the liquid outlet end of the second nutrient liquid chamber, the liquid inlet end of the sixth peristaltic pump is connected to the liquid outlet end of the second reaction liquid chamber, and the liquid outlet ends of the fifth peristaltic pump and the sixth peristaltic pump are respectively connected to the liquid inlet end of the second mixing chamber; the liquid inlet end of the seventh peristaltic pump is connected to the liquid outlet end of the second mixing chamber, and the liquid outlet end of the seventh peristaltic pump is respectively connected to the liquid inlet ends of a plurality of third reaction units.

8. The device according to claim 7, characterized in that The second reaction unit comprises a second solenoid valve and a second sample reaction chamber; the liquid inlet end of the second solenoid valve is connected to the liquid outlet ends of the first reaction liquid chamber and the second buffer chamber respectively, and the liquid outlet end of the second solenoid valve is connected to the liquid inlet end of the second sample reaction chamber; the second sample reaction chamber is pre-placed with a second reaction sample; The third reaction unit comprises a third solenoid valve and a third sample reaction chamber; the liquid inlet end of the third solenoid valve is connected to the liquid outlet end of the seventh peristaltic pump, and the liquid outlet end of the third solenoid valve is connected to the liquid inlet end of the third sample reaction chamber; the third sample reaction chamber is pre-installed with a third reaction sample; The input ends of the second solenoid valve and the third solenoid valve are electrically connected to the output end of the control module respectively.

9. The device according to claim 1, characterized in that The observation module includes a motion bearing component, a microscopic imaging component, and a light source component; The microscopic imaging component is arranged on the motion component, and the motion component drives the microscopic imaging component to perform linear reciprocating motion, so as to realize observation of different areas in the chip card box; The microscopic imaging assembly includes an objective lens, a reflector, a microscope tube and a camera; the objective lens is arranged below the chip card box; a perforation is arranged on the top of the reflector, and a laser inlet is arranged on the side of the reflector; the objective lens is located directly above the perforation, one end of the microscope tube is sealed and connected to the reflector, and the other end of the microscope tube is sealed and connected to the camera lens, so as to capture a microscopic image in the chip card box; The light source assembly includes an excitation light source unit, a catalytic light source unit and a laser switching unit; The excitation light source unit is arranged at the laser entrance of the reflector to generate excitation light of at least two wavelengths; the laser switching unit is connected to the excitation light source unit to switch excitation light of different wavelengths; the catalytic light source unit is arranged around the objective lens to generate catalytic light of at least two wavelengths.

10. The device according to claim 1, characterized in that The control module includes an integrated circuit board, which is arranged in the device body and has at least two positioning pins; the card box housing is provided with at least two guide holes corresponding to the positions of the positioning pins, and the positioning pins are inserted into the corresponding guide holes to fix the chip card box; The card box housing is provided with a control interface, the input end of the control interface is electrically connected to the output end of the integrated circuit board, and the output end of the control interface is electrically connected to the input end of the chip assembly; The device body is provided with a communication interface and a power supply interface; the communication interface is electrically connected to the control module to realize data interaction with external equipment; the power supply interface is electrically connected to the control module to provide power and information exchange.

Images