Intestinal flora detection method and device

Abstract

The invention discloses an intestinal flora detection method and device, and relates to the technical field of intestinal flora detection.The intestinal flora detection device comprises a supporting frame, the side face of the supporting frame is provided with a microscope and a supporting plate, the upper portion of the supporting frame is provided with a collecting box, the inner side of the supporting plate is slidably provided with a moving plate, and the moving plate is provided with a clamping assembly; the clamping assembly comprises a worm and a rotating rod which are rotationally connected to the moving plate, and a plurality of clamping plates movably connected to the rotating rod, the worm drives the protective shell to rotate when rotating, and the protective shell can drive the plurality of clamping plates to rotate in the direction close to the moving plate to clamp the glass slide when rotating. The movable plate enables the multiple clamping plates to conduct detection through a microscope through movement of the movable plate, and a collecting assembly is arranged on the supporting plate. Manual operation is reduced through an automatic process, the detection process is more efficient, it is ensured that the detection process of each sample is standardized, and the result consistency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of intestinal flora detection, and in particular to an intestinal flora detection method and device. Background Art

[0002] The intestinal flora refers to the microbial community living in the human intestine, including bacteria, viruses, fungi and archaea, among which bacteria account for the vast majority. These microorganisms form a complex symbiotic relationship with the human body and play a vital role in maintaining human health. The state of the intestinal flora is closely related to human health. By monitoring the intestinal flora, we can understand the health status of the human body and detect potential disease risks in a timely manner. The microscope is one of the important tools for studying the intestinal flora. Through the microscope, the morphology, structure and distribution of the intestinal flora can be intuitively observed.

[0003] After searching, the invention patent with Chinese patent number CN118778239A discloses an optical microscope with a stable anti-deflection structure. Compared with the prior art, the invention patent with Chinese patent number CN118778239A realizes stable positioning of the side wall of the thin slice through the No. 2 anti-deflection plate, and at the same time, the No. 1 anti-deflection plate will realize stable positioning of the top wall of the thin slice under the action of the supporting spring, so that the optical microscope can realize anti-deflection operation of the thin slice through the No. 1 anti-deflection plate and the No. 2 anti-deflection plate, thereby preventing the staff from observing the object from being affected, and the operation process is simple and quick.

[0004] However, in actual use of the above device, when multiple stool samples need to be tested, the staff needs to repeatedly fix, disassemble and collect the slide samples. The process is cumbersome and the detection efficiency is low. Therefore, a method and device for detecting intestinal flora are proposed. Summary of the invention

[0005] The purpose of the present invention is to solve the shortcomings of the prior art that the process of repeatedly installing, disassembling and collecting slides is cumbersome and leads to low detection efficiency, and to propose a method and device for detecting intestinal flora.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A device for detecting intestinal flora comprises a support frame, a microscope and a support plate are respectively installed on the sides of the support frame, a collecting box is installed on the upper part of the support frame, a movable plate is slidably arranged on the inner side of the support plate, and different slides can be driven for monitoring by sliding the movable plate, a clamping assembly is arranged on the movable plate, the clamping assembly comprises a worm and a rotating rod rotatably connected to the movable plate, and a plurality of clamping plates movably connected to the rotating rod, when the worm rotates, the rotating rod is driven to rotate, and when the rotating rod rotates, the plurality of clamping plates can be driven to rotate in a direction close to the movable plate to clamp the slides, and the movable plate is clamped by its own The movement enables multiple clamping plates to be inspected separately through a microscope, and the automated process reduces manual operations, making the inspection process more efficient. A collecting assembly is provided on the support plate, and the collecting assembly includes an extrusion plate installed on the support plate, and a pushing rod movably connected to the support plate. Multiple collecting grooves are provided inside the movable plate. When the movable plate moves, the clamping plate can be squeezed by the extrusion plate. After being squeezed, the clamping plate generates an upward force and is separated from the slide. When the pushing rod moves, it pushes the slide through the collecting groove and falls into the collection box, which reduces the manual handling of the slide after inspection and improves work efficiency.

[0008] The above technical solution further includes:

[0009] The support plate has a first opening groove inside, the movable plate is slidably arranged inside the first opening groove, and the size of the first opening groove is adapted to the size of the movable plate, so that the movable plate can slide stably along the first opening groove.

[0010] A square groove is opened on the upper part of the movable plate, the worm is rotatably connected to the inner side of the square groove, a protective shell is installed on the upper part of the movable plate, the rotating rod is rotatably connected to the inner side of the protective shell, a worm wheel is installed on the outer side of the rotating rod, and the worm wheel is meshed with the worm, so that the worm wheel can be driven to rotate by the worm wheel when the worm is rotated.

[0011] A plurality of square blocks are installed on the outer side of the rotating rod, and the square blocks are movably connected to the clamping plate. A rubber plate is installed on the side of the clamping plate close to the movable plate, and the rubber plate can increase the friction with the glass slide. The elasticity of the rubber plate can be used to fix glass slides of different thicknesses.

[0012] A second opening groove is provided inside the clamping plate, and the pressing plate is located on the movement track between the clamping plate and the edge of the second opening groove. The pressing plate can press the clamping plate to separate it from the slide.

[0013] A first sliding groove is provided on a side of the square block close to the clamping plate, a first sliding block is slidingly arranged inside the first sliding groove, a first spring is installed on the upper part of the first sliding block, an end of the first spring away from the first sliding block is fixedly connected to the inner wall of the first sliding groove, a side of the first sliding block away from the rotating rod is fixedly connected to the clamping plate, and when the clamping plate moves, it can move along the first sliding groove through the first sliding block.

[0014] A rack is installed at the bottom of the movable plate, and a rotating handle is rotatably connected to the bottom of the support plate. A gear is installed at one end of the rotating handle close to the rack. The gear is meshed with the rack. Rotating the rotating handle can drive the movable plate to move through the gear and the rack.

[0015] A second sliding groove is provided on the upper part of the support plate, a second sliding block is slidingly arranged inside the second sliding groove, a second spring is installed on the side of the second sliding block, an end of the second spring away from the second sliding block is fixedly connected to the inner wall of the second sliding groove, a movable block is movably connected to the upper part of the second sliding block, and a side of the movable block close to the clamping plate is fixedly connected to the pushing rod, so that the pushing rod moves horizontally to push the slide.

[0016] A square rod and a third spring are respectively installed on the upper part of the second sliding block. The square rod is slidably connected to the movable block. One end of the third spring away from the second sliding block is fixedly connected to the movable block. Pressing the movable block can make the push rod contact the moving plate.

[0017] A method used by an intestinal flora detection device comprises the following steps:

[0018] Step 1: Rotate the worm to drive the clamping plate on the rotating rod to rotate in a direction away from the moving plate, place multiple glass slides on the clamping plates respectively, and reverse the worm to fix the multiple glass slides simultaneously through the multiple clamping plates;

[0019] Step 2: Move the moving plate, and the moving plate drives the glass slides at different clamping plates to move to the bottom of the microscope for detection;

[0020] Step 3: The tested slide is moved to the squeezing plate through the moving plate, and the clamping plate is squeezed by the squeezing plate, so that the clamping plate is separated from the slide, and the push rod pushes the slide, so that the slide falls through the collection slot into the collection box for automatic collection.

[0021] The present invention has the following beneficial effects:

[0022] 1. In the present invention, a plurality of glass slides are clamped simultaneously and quickly by a clamping assembly, and then the glass slides are moved to the bottom of the microscope for detection by the movement of a movable plate. The automated process reduces manual operations, making the detection process more efficient, ensuring that the detection process of each sample is standardized, and improving the consistency of the results.

[0023] 2. In the present invention, the collection assembly can quickly collect the glass slides after the test is completed, which reduces the manual handling of the glass slides after the test, improves work efficiency, and also helps prevent sample leakage and contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the overall side structure of an intestinal flora detection method and device proposed by the present invention;

[0025] Figure 2 It is a schematic diagram of the overall side structure of the present invention;

[0026] Figure 3 It is a schematic diagram of the connection structure between the square block and the clamping plate in the present invention;

[0027] Figure 4 It is a schematic diagram of the structure of the extruded plate in the present invention;

[0028] Figure 5 for Figure 1 A schematic diagram of the structure enlargement in the middle;

[0029] Figure 6 for Figure 1 A magnified schematic diagram of the structure at B in the middle;

[0030] Figure 7 for Figure 2 A magnified schematic diagram of the structure at C in the middle;

[0031] Figure 8 for Figure 2 A magnified schematic diagram of the structure at D in the middle;

[0032] Fig. 9 for Figure 3 Enlarged schematic diagram of the structure at point E in the middle.

[0033] In the figure: 1. support frame; 2. microscope; 3. support plate; 4. first opening groove; 5. moving plate; 6. rack; 7. turning handle; 8. gear; 9. square groove; 10. worm; 11. protective shell; 12. rotating rod; 13. worm wheel; 14. square block; 15. clamping plate; 16. second opening groove; 17. rubber plate; 18. first sliding groove; 19. first sliding block; 20. first spring; 21. extrusion plate; 22. collecting groove; 23. second sliding groove; 24. second sliding block; 25. second spring; 26. square rod; 27. third spring; 28. movable block; 29. ​​push rod; 30. collecting box. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Embodiment 1

[0036] like Figure 1-Figure 9 As shown, an intestinal flora detection device proposed by the present invention comprises a support frame 1, a microscope 2 and a support plate 3 are respectively installed on the sides of the support frame 1, a collecting box 30 is installed on the upper part of the support frame 1, a movable plate 5 is slidably arranged on the inner side of the support plate 3, and different slides can be driven for monitoring by sliding the movable plate 5, a clamping assembly is arranged on the movable plate 5, and the clamping assembly comprises a worm 10 and a rotating rod 12 rotatably connected to the movable plate 5, and a plurality of clamping plates 15 movably connected to the rotating rod 12, when the worm 10 rotates, the rotating rod 12 is driven to rotate, and when the rotating rod 12 rotates, the plurality of clamping plates 15 can be driven to rotate in a direction close to the movable plate 5 to clamp the slides, and the movable plate 5 is provided with a clamping assembly ... The plate 5 enables multiple clamping plates 15 to be inspected through the microscope 2 respectively through its own movement. The automated process reduces manual operations and makes the inspection process more efficient. A collecting component is provided on the support plate 3, and the collecting component includes an extrusion plate 21 installed on the support plate 3, and a pushing rod 29 movably connected to the support plate 3. A plurality of collecting grooves 22 are opened inside the movable plate 5. When the movable plate 5 moves, the clamping plate 15 can be squeezed by the extrusion plate 21. After being squeezed, the clamping plate 15 generates an upward force to separate from the slide. When the pushing rod 29 moves, it pushes the slide through the collecting groove 22 and falls into the collection box 30, which reduces the manual handling of the slide after inspection and improves work efficiency.

[0037] The support plate 3 has a first opening groove 4 inside, and the movable plate 5 is slidably arranged inside the first opening groove 4. The size of the opening of the first opening groove 4 matches the size of the movable plate 5, so that the movable plate 5 can slide stably along the first opening groove 4.

[0038] A square groove 9 is opened on the upper part of the movable plate 5, and the worm 10 is rotatably connected to the inner side of the square groove 9. A protective shell 11 is installed on the upper part of the movable plate 5, and the rotating rod 12 is rotatably connected to the inner side of the protective shell 11. A worm wheel 13 is installed on the outer side of the rotating rod 12, and the worm wheel 13 is meshed with the worm 10. When the worm 10 is rotated, the worm wheel 13 can be driven to rotate by the worm wheel 13.

[0039] A plurality of square blocks 14 are installed on the outer side of the rotating rod 12, and the square blocks 14 are movably connected to the clamping plate 15. A rubber plate 17 is installed on the side of the clamping plate 15 close to the movable plate 5. The rubber plate 17 can increase the friction with the glass slide. The elasticity of the rubber plate 17 can be used to fix glass slides of different thicknesses.

[0040] A rack 6 is installed at the bottom of the movable plate 5, and a rotating handle 7 is rotatably connected to the bottom of the support plate 3. A gear 8 is installed at one end of the rotating handle 7 close to the rack 6. The gear 8 is meshed with the rack 6. Rotating the rotating handle 7 can drive the movable plate 5 to move through the gear 8 and the rack 6.

[0041] In this embodiment, when it is necessary to perform intestinal flora detection, the sample can be firstly smeared on the glass slide, and the worm 10 can be rotated at this time. Since the worm 10 is meshed with the worm wheel 13, the worm 10 can drive the rotating rod 12 to rotate through the worm wheel 13 when rotating, and the rotating rod 12 can drive the clamping plate 15 to rotate in the direction away from the movable plate 5 when rotating. At this time, different glass slides can be placed at the positions corresponding to the plurality of clamping plates 15, and then the worm 10 is reversed, and the rotating rod 12 is reversed by the worm 10, so that the clamping plate 15 is reversed, and then the glass slide is clamped, and the clamping plate 15 contacts the glass slide through the rubber plate 17, which can increase the friction force, and the elasticity of the rubber plate 17 can be used to fix glass slides of different thicknesses, so that multiple glass slides can be quickly fixed;

[0042] Then, the rotating handle 7 is turned to drive the gear 8 to rotate. Since the gear 8 is meshed with the rack 6, the rotating handle 7 can drive the movable plate 5 to move along the first open groove 4 through the gear 8 and the rack 6 until the glass slide is moved to the bottom of the microscope 2. Then, the detection work is carried out. According to the total number of bacteria observed, morphological characteristics, the ratio of Gram-positive and Gram-negative bacteria, the ratio of bacilli to cocci, and whether there is an increase in bacteria of special morphology, etc., a comprehensive judgment is made as to whether there is dysbiosis. After the sample on the current glass slide is detected, the rotating handle 7 is then turned to drive the movable plate 5 to move until the next glass slide is moved to the bottom of the microscope 2 for detection. In this way, multiple glass slides are quickly fixed, and then the movable plate 5 is moved to drive different glass slides to the bottom of the microscope 2 for detection. The automated process reduces manual operation, makes the detection process more efficient, ensures that the detection process of each sample is standardized, and improves the consistency of the results.

[0043] Embodiment 2

[0044] like Figure 1-Figure 9 As shown, based on the first embodiment, a second opening groove 16 is opened inside the clamping plate 15, and the squeezing plate 21 is located on the movement trajectory of the clamping plate 15 and the edge of the second opening groove 16. The squeezing plate 21 can squeeze the clamping plate 15 to separate it from the slide.

[0045] A first sliding groove 18 is provided on one side of the square block 14 close to the clamping plate 15, a first sliding block 19 is slidingly provided inside the first sliding groove 18, a first spring 20 is installed on the upper part of the first sliding block 19, an end of the first spring 20 away from the first sliding block 19 is fixedly connected to the inner wall of the first sliding groove 18, a side of the first sliding block 19 away from the rotating rod 12 is fixedly connected to the clamping plate 15, and when the clamping plate 15 moves, it can move along the first sliding groove 18 through the first sliding block 19.

[0046] A second sliding groove 23 is provided on the upper portion of the support plate 3, a second sliding block 24 is slidingly provided inside the second sliding groove 23, a second spring 25 is installed on the side of the second sliding block 24, an end of the second spring 25 away from the second sliding block 24 is fixedly connected to the inner wall of the second sliding groove 23, a movable block 28 is movably connected to the upper portion of the second sliding block 24, a side of the movable block 28 close to the clamping plate 15 is fixedly connected to a pushing rod 29, so that the pushing rod 29 moves horizontally to push the slide.

[0047] A square rod 26 and a third spring 27 are respectively installed on the upper part of the second sliding block 24. The square rod 26 is slidably connected to the movable block 28. The end of the third spring 27 away from the second sliding block 24 is fixedly connected to the movable block 28. Pressing the movable block 28 can make the push rod 29 contact with the movable plate 5.

[0048] In this embodiment, after the sample detection of the current glass slide is completed, the next glass slide is detected by moving the moving plate 5. At this time, the clamping plate 15 fixed to the previous glass slide moves to the squeezing plate 21, and then the squeezing plate 21 squeezes the inclined surface at the connection between the clamping plate 15 and the second opening groove 16 until the squeezing plate 21 moves to the inside of the second opening groove 16. At this time, the clamping plate 15 moves along the first sliding groove 18 through the first sliding block 19 through the squeezing force, and the first spring 20 contracts. At this time, the clamping plate 15 drives the rubber plate 17 to move upward, and then automatically releases the fixation of the glass slide. At this time, the movable block 28 can be pressed to move the movable block 28 down to the second sliding groove 2 3, at this time, the third spring 27 contracts, and the movable block 28 moves downward, driving the push rod 29 to move. At this time, the push rod 29 contacts the movable plate 5, and then pushes the second sliding block 24 through the movable block 28, so that the second sliding block 24 moves along the second sliding groove 23. At this time, the second spring 25 contracts, and the second sliding block 24 moves to and can drive the push rod 29 to move through the movable block 28. The push rod 29 moves to and can push the slide, and push the slide to the collecting groove 22, so that the slide falls into the collecting box 30 through the collecting groove 22, so that the slide is quickly collected after the detection is completed, which reduces the manual handling of the slide after the detection, improves the work efficiency, and also helps to prevent sample leakage and contamination.

[0049] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An intestinal flora detection device, comprising a support frame (1), characterized in that: A microscope (2) and a support plate (3) are respectively installed on the side of the support frame (1); a collection box (30) is installed on the upper part of the support frame (1); a movable plate (5) is slidably arranged on the inner side of the support plate (3); a clamping assembly is arranged on the movable plate (5); the clamping assembly comprises a worm (10) and a rotating rod (12) rotatably connected to the movable plate (5), and a plurality of clamping plates (15) movably connected to the rotating rod (12); when the worm (10) rotates, the rotating rod (12) is driven to rotate; when the rotating rod (12) rotates, the plurality of clamping plates (15) are driven to rotate in a direction close to the movable plate (5) to clamp the glass slide; The plate (5) enables a plurality of clamping plates (15) to be inspected respectively through a microscope (2) by its own movement. A collecting assembly is arranged on the support plate (3), and the collecting assembly comprises a squeezing plate (21) mounted on the support plate (3) and a pushing rod (29) movably connected to the support plate (3). A plurality of collecting grooves (22) are provided inside the movable plate (5). When the movable plate (5) moves, the squeezing plate (21) can squeeze the clamping plate (15). After being squeezed, the clamping plate (15) generates an upward force to separate from the glass slide. When the pushing rod (29) moves, it pushes the glass slide to fall into the collection box (30) through the collecting groove (22).

2. The intestinal flora detection device according to claim 1, characterized in that: The support plate (3) is provided with a first opening groove (4) inside, the movable plate (5) is slidably arranged inside the first opening groove (4), and the size of the opening of the first opening groove (4) is adapted to the size of the movable plate (5).

3. The intestinal flora detection device according to claim 2, characterized in that: A square groove (9) is provided on the upper part of the movable plate (5), the worm (10) is rotatably connected to the inner side of the square groove (9), a protective shell (11) is installed on the upper part of the movable plate (5), the rotating rod (12) is rotatably connected to the inner side of the protective shell (11), a worm wheel (13) is installed on the outer side of the rotating rod (12), and the worm wheel (13) is meshed with the worm (10).

4. The intestinal flora detection device according to claim 1, characterized in that: A plurality of square blocks (14) are installed on the outer side of the rotating rod (12), and the square blocks (14) are movably connected to the clamping plate (15). A rubber plate (17) is installed on the side of the clamping plate (15) close to the moving plate (5).

5. The intestinal flora detection device according to claim 4, characterized in that: A second opening groove (16) is provided inside the clamping plate (15), and the extrusion plate (21) is located on a movement track at the edge of the clamping plate (15) and the second opening groove (16).

6. The intestinal flora detection device according to claim 4, characterized in that: A first sliding groove (18) is provided on a side of the square block (14) close to the clamping plate (15), a first sliding block (19) is slidably arranged inside the first sliding groove (18), a first spring (20) is installed on the upper part of the first sliding block (19), an end of the first spring (20) away from the first sliding block (19) is fixedly connected to the inner wall of the first sliding groove (18), and a side of the first sliding block (19) away from the rotating rod (12) is fixedly connected to the clamping plate (15).

7. The intestinal flora detection device according to claim 1, characterized in that: A rack (6) is installed at the bottom of the movable plate (5), and a rotating handle (7) is rotatably connected to the bottom of the support plate (3). A gear (8) is installed at one end of the rotating handle (7) close to the rack (6), and the gear (8) is meshed with the rack (6).

8. The intestinal flora detection device according to claim 1, characterized in that: A second sliding groove (23) is provided on the upper part of the support plate (3), a second sliding block (24) is slidably arranged inside the second sliding groove (23), a second spring (25) is installed on the side of the second sliding block (24), an end of the second spring (25) away from the second sliding block (24) is fixedly connected to the inner wall of the second sliding groove (23), a movable block (28) is movably connected to the upper part of the second sliding block (24), and a side of the movable block (28) close to the clamping plate (15) is fixedly connected to the push rod (29).

9. The intestinal flora detection device according to claim 8, characterized in that: A square rod (26) and a third spring (27) are respectively installed on the upper part of the second sliding block (24); the square rod (26) is slidably connected to the movable block (28); and one end of the third spring (27) away from the second sliding block (24) is fixedly connected to the movable block (28).

10. A method used in a device for detecting intestinal flora according to claims 1-9, characterized in that: The steps include: Step 1: Rotate the worm (10) to drive the clamping plate (15) on the rotating rod (12) to rotate in a direction away from the movable plate (5), place a plurality of glass slides on the clamping plates (15), and reverse the worm (10) to fix the plurality of glass slides simultaneously through the plurality of clamping plates (15); Step 2: Move the movable plate (5), and the movable plate (5) drives the glass slides at different clamping plates (15) to move to the bottom of the microscope (2) for detection; Step 3: The glass slide that has been tested is moved to the squeezing plate (21) through the moving plate (5), and the squeezing plate (21) squeezes the clamping plate (15), so that the clamping plate (15) is separated from the glass slide, and the push rod (29) pushes the glass slide, so that the glass slide falls through the collecting groove (22) to the collecting box (30) for automatic collection.

Images