Preparation method of bimetallic selenide, non-enzymatic electrochemical sensor of bimetallic selenide and application of non-enzymatic electrochemical sensor

Abstract

The invention relates to the technical field of pollutant detection, and discloses a preparation method of bimetallic selenide and a non-enzymatic electrochemical sensor and application of the non-enzymatic electrochemical sensor, a reference electrode of the non-enzymatic electrochemical sensor is a silver / silver chloride electrode, an auxiliary electrode is a platinum wire electrode, and a working electrode is a glassy carbon composite electrode containing a bimetallic selenide coating. The bimetallic selenide is CuInSe2, and the CuInSe2 contained in the bimetallic selenide has the sensitivity of identifying parathion. The parathion detection accuracy and operation convenience can be improved, and the parathion detection cost is reduced. According to the preparation method of the bimetallic selenide, ethylamine and N, N-dimethylformamide serve as solvents, cuprous chloride, indium chloride and selenium powder react to generate CuInSe2 through solvothermal reaction, and the preparation method has the advantages of being high in reaction efficiency and low in manufacturing cost.

Description

Technical Field

[0001] The present invention relates to the technical field of pollutant detection, and in particular to a preparation method of a bimetallic selenide and a non-enzymatic electrochemical sensor and application thereof. Background Art

[0002] Organophosphorus pesticides can be used as highly effective insecticides, fungicides, herbicides and plant growth regulators, and have been widely used in controlling crop pests and diseases and increasing grain production.

[0003] The insecticidal ingredient in organophosphorus pesticides is parathion, which has a broad spectrum of high toxicity and can enter the human body through the digestive tract, skin mucosa and respiratory tract. It will inhibit the activity of acetylcholinesterase in the human body, affect the nervous system function of the brain, cause respiratory system disorders in the human body, and even lead to death.

[0004] In the prior art, organophosphorus pesticide residue detection methods for detecting the content of parathion include chromatography detection methods, enzyme inhibition detection methods and immunological detection methods.

[0005] Chromatographic detection methods are divided into high performance liquid chromatography, gas chromatography and chromatography-mass spectrometry. These chromatographic detection methods have the defects of high equipment requirements, expensive equipment, cumbersome sample processing and long detection cycle, and require professional personnel to operate and maintain, resulting in insufficient convenience of detection operation.

[0006] Enzyme inhibition detection methods are divided into rapid test card method and spectrophotometry method. These enzyme inhibition detection methods are easily interfered by the external environment and have the defects of easily producing false positive results.

[0007] Immunoassay methods are divided into enzyme-linked immunosorbent assay, fluorescent immunoassay and chemiluminescent immunoassay. These immunoassay methods require the use of colloidal gold and are very expensive. Summary of the invention

[0008] In view of the above problems, the first object of the present invention is to provide a non-enzymatic electrochemical sensor to solve the problems of insufficient detection accuracy, low operational convenience and high detection cost of parathion.

[0009] Furthermore, the second object of the present invention is to provide a method for preparing bimetallic selenide to obtain CuInSe2 with low manufacturing cost.

[0010] Furthermore, the third object of the present invention is to propose a method for applying bimetallic selenide of CuInSe2 to obtain a non-enzymatic electrochemical sensor with good operating convenience and detection sensitivity.

[0011] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0012] A non-enzymatic electrochemical sensor using a bimetallic selenide, comprising a working electrode, an auxiliary electrode and a reference electrode;

[0013] The reference electrode is a silver / silver chloride electrode, the auxiliary electrode is a platinum wire electrode, and the working electrode is a glassy carbon composite electrode containing a bimetallic selenide coating;

[0014] The bimetallic selenide is CuInSe2, and the contained CuInSe2 has the sensitivity to identify parathion. Specifically, the diameter of the glassy carbon composite electrode is 5 mm, and the content of CuInSe2 in the coating is 0.08-0.15 mg.

[0015] Furthermore, the present invention provides a method for preparing a bimetallic selenide, wherein the prepared bimetallic selenide is used for preparing the above-mentioned non-enzymatic electrochemical sensor using the bimetallic selenide, comprising the following steps:

[0016] S1) placing an N,N-dimethylformamide solution under inert gas protection, and adding cuprous chloride and indium chloride respectively during stirring to obtain a first solution;

[0017] S2) placing the N,N-dimethylformamide solution under inert gas protection, and adding selenium powder and ethylamine respectively while stirring to obtain a second solution;

[0018] S3) under inert gas protection and magnetic stirring, adding the first solution and the second solution into a reaction container respectively, mixing them evenly, to obtain a reaction solution;

[0019] S4) slowly heating the reaction solution to the reaction temperature, keeping the temperature until the reaction stops, naturally cooling to room temperature, washing three times with anhydrous ethanol, and drying to obtain the bimetallic selenide with a hexagonal structure.

[0020] Preferably, the temperature of the N,N-dimethylformamide solution in step S1) and step S2) is 120°C.

[0021] Preferably, the molar concentration ratio of cuprous chloride, indium chloride and selenium powder is 1:1:1.

[0022] Preferably, in step S2), the volume ratio of the molar concentration of selenium powder to ethylamine is 1 mmol:1 mL.

[0023] An appropriate amount of ethylamine was added as a solvent to dissolve the selenium powder.

[0024] Preferably, in step S4), the reaction temperature is 220-260°C.

[0025] Preferably, in step S4), the drying temperature is 60° C. and the drying time is 24 h.

[0026] Preferably, the inert protective gas is nitrogen or helium.

[0027] Furthermore, the present invention also proposes an application of a bimetallic selenide, wherein the bimetallic selenide prepared by the above-mentioned preparation method of the bimetallic selenide is configured into a dispersion liquid and coated on a glassy carbon composite electrode, and the glassy carbon composite electrode is used as a working electrode of a non-enzymatic electrochemical sensor to detect parathion in organic pesticides.

[0028] The beneficial effects of the technical solution of the present invention are as follows: the non-enzymatic electrochemical sensor using bimetallic selenide uses a glassy carbon composite electrode coated with a CuInSe2 coating as a working electrode, has good sensitivity in identifying parathion, can improve the accuracy and operational convenience of detecting parathion, and reduce the detection cost of parathion.

[0029] Furthermore, the present invention proposes a method for preparing bimetallic selenide, which uses ethylamine and N,N-dimethylformamide as solvents, and through a solvothermal reaction, cuprous chloride, indium chloride and selenium powder are chelated in the reaction solution to generate CuInSe2, which has the advantages of high reaction efficiency and low manufacturing cost.

[0030] Furthermore, the application of a bimetallic selenide proposed in the present invention, in which the prepared bimetallic selenide CuInSe2 is applied to the working electrode of a non-enzymatic electrochemical sensor, can reduce the cost of detecting parathion by the non-enzymatic electrochemical sensor and improve the accuracy and ease of operation of detecting parathion by the non-enzymatic electrochemical sensor. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a SEM electron microscope scanning image of CuInSe2 prepared in Example 1 of the present invention;

[0032] Figure 2 This is the X-ray diffraction pattern of the bimetallic selenide CuInSe2 prepared in Example 1 of the present invention;

[0033] Figure 3 It is a square wave voltammetric graph of parathion detected by the non-enzymatic electrochemical sensor of Example 1, Comparative Example 1 and Comparative Example 2 of the present invention;

[0034] Figure 4 This is a square wave voltammetry graph of the non-enzymatic electrochemical sensor of Example 1 of the present invention detecting different concentrations of parathion;

[0035] Figure 5 A linear relationship diagram between the square wave voltammetric current peak value and the parathion concentration when the non-enzymatic electrochemical sensor of Example 1 of the present invention detects different concentrations of parathion. DETAILED DESCRIPTION

[0036] The technical solution of the present invention is further illustrated below through specific implementation methods.

[0037] In the description of this specification, the description with reference to the terms "embodiment", "example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0038] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

[0039] A non-enzymatic electrochemical sensor using a bimetallic selenide, comprising a working electrode, an auxiliary electrode and a reference electrode;

[0040] The reference electrode is a silver / silver chloride electrode, the auxiliary electrode is a platinum wire electrode, and the working electrode is a glassy carbon composite electrode containing a bimetallic selenide coating;

[0041] The bimetallic selenide is CuInSe2, and the contained CuInSe2 has the sensitivity to recognize parathion.

[0042] Figure 1 Shown is a SEM electron microscope scanning image of CuInSe2, which is a bimetallic selenide. The molecule of CuInSe2 is CuInSe2, which has a hexagonal structure and has the sensitivity to recognize parathion. The glassy carbon composite electrode containing CuInSe2 coating formed by coating on a glassy carbon electrode is used as a working electrode of a non-enzymatic electrochemical sensor, which can improve the sensitivity of the non-enzymatic electrochemical sensor to recognize parathion, thereby improving the accuracy and convenience of detecting parathion and reducing the detection cost of parathion.

[0043] Specifically, the diameter of the glassy carbon composite electrode is 5 mm, and the content of CuInSe2 in the coating is 0.08-0.15 mg.

[0044] The glassy carbon composite electrode can be obtained by using 0.08-0.15 mg of CuInSe2 to prepare a dispersion and coating it on a glassy carbon electrode, and the manufacturing cost is low.

[0045] Furthermore, the present invention provides a method for preparing a bimetallic selenide, wherein the prepared bimetallic selenide is used for preparing the above-mentioned non-enzymatic electrochemical sensor using the bimetallic selenide, comprising the following steps:

[0046] S1) placing an N,N-dimethylformamide solution under inert gas protection, and adding cuprous chloride and indium chloride respectively during stirring to obtain a first solution;

[0047] S2) placing the N,N-dimethylformamide solution under inert gas protection, and adding selenium powder and ethylamine respectively while stirring to obtain a second solution;

[0048] S3) under inert gas protection and magnetic stirring, adding the first solution and the second solution into a reaction container respectively, mixing them evenly, to obtain a reaction solution;

[0049] S4) slowly heating the reaction solution to the reaction temperature, keeping the temperature until the reaction stops, naturally cooling to room temperature, washing three times with anhydrous ethanol, and drying to obtain the bimetallic selenide with a hexagonal structure.

[0050] The preparation method of the bimetallic selenide of the present invention uses N,N-dimethylformamide solution and ethylamine as solvents, and generates CuInSe2 from cuprous chloride, indium chloride and selenium powder through a solvent thermal reaction, which has the advantages of high reaction efficiency and low manufacturing cost.

[0051] Preferably, the temperature of the N,N-dimethylformamide solution in step S1) and step S2) is 120°C.

[0052] Heating the N,N-dimethylformamide solution to 120°C can remove oxygen from the N,N-dimethylformamide solution and prevent the added cuprous chloride from being oxidized.

[0053] Preferably, the molar concentration ratio of cuprous chloride, indium chloride and selenium powder is 1:1:1.

[0054] Adding cuprous chloride, indium chloride and selenium powder in a molar concentration ratio of 1:1:1 can avoid material waste and reduce the generation of reaction by-products.

[0055] Preferably, in step S2), the volume ratio of the molar concentration of selenium powder to ethylamine is 1 mmol:1 mL.

[0056] An appropriate amount of ethylamine is added as a solvent for dissolving selenium powder to improve the reaction efficiency of the solvothermal reaction.

[0057] Preferably, in step S4), the reaction temperature is 220-260°C.

[0058] A reaction temperature of 220-260°C is beneficial to increasing the reaction rate of the solvothermal reaction.

[0059] Preferably, in step S4), the drying temperature is 60° C. and the drying time is 24 h.

[0060] The residual anhydrous ethanol in the obtained bimetallic selenide can be completely removed by drying at 60°C for 24 hours.

[0061] Preferably, the inert protective gas is nitrogen or helium.

[0062] Nitrogen is preferably used as the inert protective gas, which has a better manufacturing cost.

[0063] Furthermore, the present invention also proposes an application of a bimetallic selenide, wherein the bimetallic selenide prepared by the above-mentioned preparation method of the bimetallic selenide is configured into a dispersion liquid and coated on a glassy carbon composite electrode, and the glassy carbon composite electrode is used as a working electrode of a non-enzymatic electrochemical sensor to detect parathion in organic pesticides.

[0064] By applying the prepared bimetallic selenide of CuInSe2 to the working electrode of the non-enzymatic electrochemical sensor, the cost of detecting parathion by the non-enzymatic electrochemical sensor can be reduced, and the accuracy and operation convenience of detecting parathion by the non-enzymatic electrochemical sensor can be improved.

[0065] Example 1 and Comparative Examples 1-2

[0066] 1. Prepare the bimetallic selenide of Example 1 according to the following steps:

[0067] S1) placing 5 mL of 120° C. N,N-dimethylformamide solution under nitrogen protection, and adding 0.5 mmol of cuprous chloride and 0.5 mmol of indium chloride respectively during stirring to prepare a first solution;

[0068] S2) placing 5 mL of 120° C. N,N-dimethylformamide solution under nitrogen protection, and adding 0.5 mmol of selenium powder and 0.5 mL of ethylamine respectively while stirring to prepare a second solution;

[0069] S3) under nitrogen protection and magnetic stirring, adding the first solution and the second solution into the reaction container respectively, mixing them evenly, to obtain a reaction solution;

[0070] S4) The reaction solution is slowly heated to 250° C., kept warm until the reaction stops, naturally cooled to room temperature, washed three times with anhydrous ethanol, and dried at 60° C. for 24 h to obtain the bimetallic selenide CuInSe2 with a hexagonal structure of Example 1.

[0071] The SEM scanning electron microscope image of the prepared bimetallic selenide CuInSe2 is as follows: Figure 1 The X-ray diffraction pattern is shown in Figure 2 shown.

[0072] 2. Preparation of Metal Selenides of Comparative Examples 1 and 2

[0073] 2.1 The metal selenide of Comparative Example 1 was prepared according to the following steps:

[0074] S1) placing 5 mL of 120° C. N,N-dimethylformamide solution under nitrogen protection, and adding 0.5 mmol of cuprous chloride respectively during stirring to prepare a first solution;

[0075] S2) placing 5 mL of 120° C. N,N-dimethylformamide solution under nitrogen protection, and adding 0.5 mmol of selenium powder and 0.5 mL of ethylamine respectively while stirring to prepare a second solution;

[0076] S3) under nitrogen protection and magnetic stirring, adding the first solution and the second solution into the reaction container respectively, mixing them evenly, to obtain a reaction solution;

[0077] S4) slowly heating the reaction solution to 250° C., keeping the temperature until the reaction stops, naturally cooling to room temperature, washing three times with anhydrous ethanol, and drying at 60° C. for 24 h to obtain the metal selenide cuprous selenide Cu2Se of Comparative Example 1.

[0078] 2.2 The metal selenide of Comparative Example 2 was prepared according to the following steps:

[0079] S1) placing 5 mL of 120° C. N,N-dimethylformamide solution under nitrogen protection, and adding 0.5 mmol of indium chloride respectively during stirring to prepare a first solution;

[0080] S2) placing 5 mL of 120° C. N,N-dimethylformamide solution under nitrogen protection, and adding 0.5 mmol of selenium powder and 0.5 mL of ethylamine respectively while stirring to prepare a second solution;

[0081] S3) under nitrogen protection and magnetic stirring, adding the first solution and the second solution into the reaction container respectively, mixing them evenly, to obtain a reaction solution;

[0082] S4) The reaction solution is slowly heated to 250° C., kept warm until the reaction stops, naturally cooled to room temperature, washed three times with anhydrous ethanol, and dried at 60° C. for 24 h to obtain the metal selenide indium selenide In2Se3 of Comparative Example 2.

[0083] 3. Preparation of non-enzymatic electrochemical sensors of Example 1, Comparative Example 1 and Comparative Example 2

[0084] 3.1 Take three mixed solutions prepared by 300 μL of anhydrous ethanol, 600 μL of distilled water and 100 μL of 5% nafion solution, and add 10 mg of the bimetallic selenide CuInSe2 prepared in Example 1, the metal selenide Cu2Se prepared in Comparative Example 1 and the metal selenide In2Se3 prepared in Comparative Example 2, respectively, and then ultrasonically disperse them at an ultrasonic power of 180 W for 2 h to prepare dispersions of Example 1, Comparative Example 1 and Comparative Example 2, respectively;

[0085] 3.2 Using a 10 μL Eppendorf pipette, 10 μL of the dispersions of Example 1, Comparative Example 1 and Comparative Example 2 were respectively transferred and dropped onto the surfaces of three clean glassy carbon electrodes with a diameter of 5 mm that were polished with 0.05 μm Al2O3 powder and cleaned with ultrasound, and naturally air-dried for 24 h to obtain glassy carbon composite electrodes of Example 1, Comparative Example 1 and Comparative Example 2, respectively;

[0086] 3.3 Using a platinum wire electrode as an auxiliary electrode, a silver / silver chloride electrode as a reference electrode, and the glassy carbon composite electrodes of Example 1, Comparative Example 1 and Comparative Example 2 as working electrodes, the non-enzymatic electrochemical sensors of Example 1, Comparative Example 1 and Comparative Example 2 were prepared.

[0087] 4. Comparison of the detection sensitivity of the non-enzymatic electrochemical sensors of Example 1, Comparative Example 1 and Comparative Example 2

[0088] 4.1 Prepare a comparison test solution by preparing a 0.1 mol / L phosphate buffer solution with a pH value of 8.0 and a parathion concentration of 10 μM;

[0089] 4.2 The working electrodes of the non-enzymatic electrochemical sensors of Example 1, Comparative Example 1 and Comparative Example 2 were placed in the comparison detection solution respectively, and after being immersed for 240 seconds under the condition of an electrode potential of -0.5 V, the response current of the non-enzymatic electrochemical sensors of Example 1, Comparative Example 1 and Comparative Example 2 to parathion in the comparison detection solution was recorded according to the square wave voltammetry method, and the corresponding standard curve was drawn. The results are shown in FIG. Figure 3 shown.

[0090] from Figure 3 It can be seen that the peak current value of the standard curve of Example 1 is significantly higher than the peak current values ​​of the standard curves of Comparative Examples 1 and 2, indicating that the bimetallic selenide CuInSe2 / glassy carbon composite electrode has better sensitivity for detecting parathion.

[0091] 5. Detection of different concentrations of parathion using the non-enzymatic electrochemical sensor of Example 1

[0092] 5.1 Prepare seven test solutions by preparing 0.1 mol / L phosphate buffer solutions with a pH value of 8.0 and containing parathion at concentrations of 0.5 μM, 1 μM, 5 μM, 10 μM, 50 μM, 100 μM, and 200 μM, respectively;

[0093] 5.2 After soaking for 240 seconds at an electrode potential of -0.5 V, the working electrodes of the non-enzymatic electrochemical sensor of Example 1 were placed in seven detection solutions, respectively, and the response currents of the corresponding parathion detection solutions were recorded by square wave voltammetry, and the corresponding standard curves were drawn. The results are shown in FIG. Figure 4 shown.

[0094] 5.3 According to the values ​​of parathion concentration and corresponding peak current value detected in Example 1 above, the linear regression equation obtained is: y=8.765(±0.850)x+9.341(±2.227), R 2 =0.9461, where y is the current value and x is the logarithmic value of the concentration of parathion. Figure 5 As shown, the detection limit of the non-enzymatic electrochemical sensor of Example 1 is 0.3439 μM.

[0095] Depend on Figure 4 It can be seen that each standard curve has an obvious peak current value, and as the concentration of parathion increases, the corresponding peak current value increases significantly; based on the linear relationship between the parathion concentration detected in Example 1 and the corresponding peak current value, the detection limit of the non-enzymatic electrochemical sensor of Example 1 is calculated to be 0.3439 μM, indicating that the bimetallic selenide CuInSe2 / glassy carbon composite electrode has good detection sensitivity for detecting parathion.

[0096] 6. Detection of the non-enzymatic electrochemical sensor of Example 1 in actual samples:

[0097] Acetonitrile was used to extract parathion from the apple sample, and a sample solution with a pH value of 8.0 was prepared using a phosphate buffer solution with a concentration of 0.1 mol / L. The working electrode of the non-enzymatic electrochemical sensor of Example 1 was placed in the sample solution and immersed in the solution for 240 seconds at a potential of -0.5 V. The response current value of parathion in the sample solution was determined by square wave voltammetry, and the recovery rate was 95.3%-104.5, and the RSD was less than 3%.

[0098] In actual sample detection, the non-enzymatic electrochemical sensor prepared in Example 1 of the present invention has good detection accuracy, indicating that the non-enzymatic electrochemical sensor of the present invention can be used for the determination of parathion residues in agricultural products.

[0099] In summary, the non-enzymatic electrochemical sensor using bimetallic selenide of the present invention uses a glassy carbon composite electrode coated with CuInSe2 as a working electrode, has good sensitivity in identifying parathion, can improve the accuracy and operational convenience of detecting parathion, and reduce the detection cost of parathion.

[0100] Furthermore, the present invention proposes a method for preparing bimetallic selenide, which uses ethylamine and N,N-dimethylformamide as solvents and generates CuInSe2 from cuprous chloride, indium chloride and selenium powder through a solvothermal reaction, and has the advantages of high reaction efficiency and low manufacturing cost.

[0101] Furthermore, the application of a bimetallic selenide proposed in the present invention, in which the prepared bimetallic selenide CuInSe2 is applied to the working electrode of a non-enzymatic electrochemical sensor, can reduce the cost of detecting parathion by the non-enzymatic electrochemical sensor and improve the accuracy and ease of operation of detecting parathion by the non-enzymatic electrochemical sensor.

[0102] The technical principle of the present invention is described above in conjunction with specific embodiments. These descriptions are only for explaining the principle of the present invention and cannot be interpreted as limiting the scope of protection of the present invention in any way. Based on the explanations herein, those skilled in the art can associate other specific implementations of the present invention without paying creative labor, and these methods will fall within the scope of protection of the present invention.

Claims

1. A non-enzymatic electrochemical sensor using a bimetallic selenide, comprising a working electrode, an auxiliary electrode and a reference electrode, characterized in that: The reference electrode is a silver / silver chloride electrode, the auxiliary electrode is a platinum wire electrode, and the working electrode is a glassy carbon composite electrode containing a bimetallic selenide coating; The bimetallic selenide is CuInSe2, and the contained CuInSe2 has the sensitivity to recognize parathion.

2. The non-enzymatic electrochemical sensor using bimetallic selenide according to claim 1, characterized in that: The diameter of the glassy carbon composite electrode is 5 mm, and the content of CuInSe2 in the coating is 0.08-0.15 mg.

3. A method for preparing a bimetallic selenide, characterized in that: The prepared bimetallic selenide is used for the preparation of the non-enzymatic electrochemical sensor using bimetallic selenide according to claim 1 or 2, comprising the following steps: S1) placing an N,N-dimethylformamide solution under inert gas protection, and adding cuprous chloride and indium chloride respectively during stirring to obtain a first solution; S2) placing the N,N-dimethylformamide solution under inert gas protection, and adding selenium powder and ethylamine respectively while stirring to obtain a second solution; S3) under inert gas protection and magnetic stirring, adding the first solution and the second solution into a reaction container respectively, mixing them evenly, to obtain a reaction solution; S4) slowly heating the reaction solution to the reaction temperature, keeping the temperature until the reaction stops, naturally cooling to room temperature, washing three times with anhydrous ethanol, and drying to obtain the bimetallic selenide with a hexagonal structure.

4. The method for preparing the bimetallic selenide according to claim 3, characterized in that: The temperature of the N,N-dimethylformamide solution in step S1) and step S2) is 120°C.

5. The method for preparing the bimetallic selenide according to claim 3, characterized in that: The molar concentration ratio of cuprous chloride, indium chloride and selenium powder is 1:1:

1.

6. The method for preparing the bimetallic selenide according to claim 3, characterized in that: In step S2), the volume ratio of the molar concentration of selenium powder to ethylamine is 1 mmol:1 mL.

7. The method for preparing the bimetallic selenide according to claim 3, characterized in that: In step S4), the reaction temperature is 220-260°C.

8. The method for preparing the bimetallic selenide according to claim 3, characterized in that: In step S4), the drying temperature is 60° C. and the drying time is 24 h.

9. The method for preparing the bimetallic selenide according to claim 3, characterized in that: The inert protective gas is nitrogen or helium.

10. An application of a bimetallic selenide, characterized in that: The bimetallic selenide prepared by the preparation method of the bimetallic selenide according to any one of claims 3 to 9 is configured as a dispersion and coated on a glassy carbon composite electrode, and the glassy carbon composite electrode is used as a working electrode of a non-enzymatic electrochemical sensor to detect parathion in organic pesticides.

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