Bacteriostatic compound feed additive for strengthening fish physique as well as preparation method and application thereof

Abstract

The invention belongs to the field of feed additives, and particularly relates to a bacteriostatic compound feed additive for strengthening fish physique as well as a preparation method and application thereof, and the bacteriostatic compound feed additive comprises 10 parts by mass of fermentation products of dandelion and radix paeoniae alba, 3-4 parts by mass of alkali modified attapulgite and 1-1.5 parts by mass of an adhesive. According to the compound feed additive disclosed by the invention, the dandelion and the white paeony root are fermented by taking lactobacillus delbrueckii subsp. Bulgaricus, bifidobacterium adolescentis and candida tropicalis as microbial agents, and the fermentation product is matched with the alkali modified attapulgite to prepare the compound feed additive, so that the compound feed additive not only has an antibacterial effect, but also can strengthen the physique of the fish and improve the immunity of the fish; the effects of increasing the weight of the fish and improving the muscle fiber density of the fish are good, so that the fish is tender and elastic in meat quality and good in mouth feel.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, and in particular to an antibacterial compound feed additive for strengthening fish physique, a preparation method and application thereof. Background Art

[0002] Feed additives refer to small or trace substances added during the production, processing and use of feed. They are used in small amounts in feed but have significant effects. Feed additives are raw materials that must be used in the modern feed industry. They have obvious effects on strengthening the nutritional value of basic feed, improving animal production performance, ensuring animal health, saving feed costs, and improving the quality of livestock products.

[0003] Traditional growth-promoting drug feed additives are mostly antibiotics, which pose serious safety issues such as drug residues.

[0004] Therefore, feed additives made from Chinese herbal medicines have unique advantages. The immune active substances contained in Chinese herbal medicines can enhance the body's immune function, improve the animal's ability to resist stress and disease, and improve animal production performance. They have the advantages of no residue and not easy to produce drug resistance. They have become a hot topic in animal nutrition research in recent years.

[0005] Based on this, the present invention is proposed. Summary of the invention

[0006] The purpose of the present invention is to provide an antibacterial compound feed additive for strengthening fish physique, a preparation method and application thereof, so as to solve the above-mentioned problems.

[0007] 1. The antibacterial compound feed additive for strengthening fish physique comprises the following raw materials in parts by weight:

[0008] Fermented product of dandelion and white peony root, 10 parts by weight;

[0009] Alkali-modified attapulgite, 3 to 4 parts by mass;

[0010] Binder: 1 to 1.5 parts by mass.

[0011] Furthermore, the preparation method of the alkali-modified attapulgite comprises the following steps:

[0012] The alkali-modified attapulgite is obtained by pouring the attapulgite into an alkali solution for ultrasonic dispersion, filtering, washing, filtering and drying.

[0013] Furthermore, the alkali solution is one or more of a sodium hydroxide solution and a potassium hydroxide solution.

[0014] Furthermore, the concentration of the sodium hydroxide solution or the potassium hydroxide solution is 2.3 mol / L.

[0015] Furthermore, the ultrasonic frequency is 20 kHz, the ultrasonic dispersion time is 40 to 60 min, and the ultrasonic dispersion temperature is 70 to 75°C.

[0016] Furthermore, the preparation method of the fermentation product of dandelion and white peony root comprises the following steps:

[0017] Step 1, weighing dandelion and white peony root in a mass ratio of 2:5, washing, drying, crushing, and mixing to obtain a first mixed material;

[0018] Step 2, dissolving the low-temperature refrigerated bacterial agent, inoculating it on the culture medium for cultivation and activation, and obtaining an activated bacterial strain; diluting the activated bacterial strain and inoculating it on the first mixed material, with an inoculation amount of 2.5%, and aerobically fermenting it in a constant temperature environment of 40° C. for 12 to 13 hours;

[0019] Step 3: After the fermentation is completed, the fermentation product is mashed to obtain a paste of dandelion and white peony root fermentation product.

[0020] Furthermore, the bacterial agent is prepared by mixing Lactobacillus delbrueckii subspecies bulgaricus, Bifidobacterium adolescentis and Candida tropicalis in a ratio of 4:2:1 in terms of the number of live bacteria.

[0021] Furthermore, the adhesive is soluble starch.

[0022] 2. The method for preparing the antibacterial compound feed additive for strengthening fish physique comprises the following steps:

[0023] Mix the adhesive with 3 to 5 times the amount of water to make an adhesive solution;

[0024] After the fermentation products of dandelion and white peony root are uniformly mixed with alkali-modified attapulgite, a binding liquid is poured in and the stirring is continued to prepare wet granules, and the wet granules are dried in a fluidized bed to obtain the granular antibacterial compound feed additive for strengthening fish physique.

[0025] 3. Application of the antibacterial compound feed additive for strengthening fish physique in the preparation of feed products for improving fish physique.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The present invention ferments dandelion and white peony root with Lactobacillus delbrueckii subspecies bulgaricus, Bifidobacterium adolescentis and Candida tropicalis as bacterial agents, and prepares the compound feed additive by combining the fermentation product with alkali-modified attapulgite. The compound feed additive not only has an antibacterial effect, but also can strengthen the physique of fish, increase the weight of fish and improve the muscle fiber density of fish, so that the meat of fish is tender and elastic with a good taste. DETAILED DESCRIPTION

[0028] The present invention is further described in detail below through specific examples.

[0029] The following detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present application.

[0030] Example 1

[0031] The antibacterial compound feed additive for strengthening fish physique comprises the following raw materials in parts by weight:

[0032] Fermented products of dandelion and white peony root, 10kg;

[0033] Alkali modified attapulgite, 3 kg;

[0034] Adhesive, 1kg.

[0035] The adhesive is soluble starch.

[0036] The preparation method of the alkali-modified attapulgite comprises the following steps:

[0037] The attapulgite is poured into a 2.3 mol / L sodium hydroxide solution for ultrasonic dispersion, the ultrasonic frequency is 20 kHz, the ultrasonic dispersion time is 40 min, and the ultrasonic dispersion temperature is 70-75° C.; thereafter, the alkali-modified attapulgite is obtained by filtering, washing, filtering, and drying.

[0038] The preparation method of the fermentation product of dandelion and white peony root comprises the following steps:

[0039] 1. Weigh dandelion and white peony root in a mass ratio of 2:5, wash, dry, crush and mix to obtain a first mixed material.

[0040] 2. Dissolve the low-temperature refrigerated bacterial agent, inoculate it on the culture medium for cultivation and activation, and obtain activated bacterial strains; dilute the activated bacterial strains and inoculate them on the first mixed material with an inoculation amount of 2.5%, and aerobically ferment them in a constant temperature environment of 40°C for 12 hours; the bacterial agent is prepared by mixing Lactobacillus delbrueckii subspecies bulgaricus, Bifidobacterium adolescentis and Candida tropicalis in a ratio of 4:2:1 in terms of the number of live bacteria.

[0041] 3. After the fermentation is completed, the fermentation product is mashed to obtain a paste of dandelion and white peony root fermentation products.

[0042] The method for preparing the antibacterial compound feed additive for strengthening fish physique comprises the following steps:

[0043] Mix the adhesive with 3 times the amount of water to make an adhesive solution;

[0044] After the fermentation products of dandelion and white peony root are uniformly mixed with alkali-modified attapulgite, a binding liquid is poured in and the stirring is continued to prepare wet granules, and the wet granules are dried in a fluidized bed to obtain the granular antibacterial compound feed additive for strengthening fish physique.

[0045] Example 2

[0046] The antibacterial compound feed additive for strengthening fish physique in this example includes the following raw materials in parts by weight:

[0047] Fermented products of dandelion and white peony root, 10kg;

[0048] Alkali modified attapulgite, 4 kg;

[0049] Adhesive, 1.5kg.

[0050] The fermentation products of dandelion and white peony root, alkali-modified attapulgite and adhesive in this example are the same as those in Example 1.

[0051] Example 3

[0052] The antibacterial compound feed additive for strengthening fish physique comprises the following raw materials in parts by weight:

[0053] Fermented products of dandelion and white peony root, 10kg;

[0054] Alkali-modified attapulgite, 3.5 kg;

[0055] Adhesive, 1.2kg.

[0056] The fermentation products of dandelion and white peony root, alkali-modified attapulgite and adhesive in this example are the same as those in Example 1.

[0057] Example 4

[0058] A customer of Dafeng Haiqiang Farm had weak physique, poor stress resistance, poor appetite, abnormal liver metabolism, and persistent damage to crucian carp after wintering. The amount of feeding could not be increased. After adding the antibacterial compound feed additive for strengthening fish physique obtained in Example 1 to the fish feed (the amount added was calculated at 800g / ton) and feeding the crucian carp for 5 days, the fish physique returned to normal, the liver was in good condition, and the appetite was vigorous.

[0059] Comparative Example 1

[0060] The only difference between this example and Example 1 is that the fermentation product of dandelion and white peony root is replaced by the water extract of dandelion and white peony root, and the rest is the same.

[0061] The water extract of dandelion and white peony root is prepared by decocting dandelion and white peony root in 5 times water for 5 hours and filtering.

[0062] Characterization test

[0063] In each pond, 25 crucian carps of similar size and healthy physique were placed, each weighing 0.3±0.05kg.

[0064] First, a 7-day adaptation phase was carried out, and fish feed was fed every day, and the feeding amount was consistent in each pool. The feed additives of Examples 1 to 2, Comparative Example 1 and Reference Example were mixed (the addition amount was calculated as 800g / ton), and the fish were fed every day. The experimental period was 2 months, and the results of weight gain rate, survival rate, muscle fiber density, etc. were measured. The experimental results are shown in Table 1.

[0065] Table 1

[0066] Weight gain rate (%) Survival rate (%) <![CDATA[Muscle fiber density (No. / mm 2 )]]> Example 1 139.2 96 131.6±3.29 Example 2 131.5 100 133.7±4.01 Comparative Example 1 115.3 88 112.6±2.58 Reference example 126.1 92 105.6±2.58

[0067] The reference example in Table 1 refers to the fish feed without any feed additives.

[0068] Comparative Example 2

[0069] Compared with Example 1, the only difference between this example is that the alkali-modified attapulgite is replaced by attapulgite, and the rest is the same.

[0070] The feed additive in this example was mixed into fish feed. After one week of feeding, it was found that about 6% of the crucian carp died due to food accumulation.

[0071] Comparative Example 3

[0072] The only difference between this example and Example 1 is that the alkali-modified attapulgite is replaced by the acid-modified attapulgite, and the rest are the same.

[0073] The preparation method of acid-modified attapulgite is as follows:

[0074] The attapulgite is poured into 1 mol / L hydrochloric acid for ultrasonic dispersion, the ultrasonic frequency is 20 kHz, the ultrasonic dispersion time is 40 min, and the ultrasonic dispersion temperature is 70-75° C.; thereafter, the attapulgite is filtered, washed, filtered, and dried to obtain the acid-modified attapulgite.

[0075] The feed additive in this example was mixed into fish feed. After one week of feeding, it was found that about 13% of the crucian carp died due to food accumulation.

[0076] Comparative Example 3

[0077] The effects of different fermentation bacteria on compound feed additives are shown in Table 2:

[0078] Table 2

[0079]

[0080] It can be seen that by using a bacterial agent made by mixing Lactobacillus delbrueckii subspecies bulgaricus, Bifidobacterium adolescentis and Candida tropicalis in a ratio of 4:2:1 of live bacteria, fermenting dandelion and white peony root, and using the fermentation products as raw materials to make feed additives for feeding fish, the muscle fiber density of fish can be significantly increased, and the fish meat can be tender and elastic with a better taste.

[0081] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0082] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. Antimicrobial compound feed additive for strengthening fish physique, characterized by: The invention comprises the following raw materials in parts by weight: Fermentation product of dandelion and white peony root, 10 parts by weight; Alkali-modified attapulgite, 3 to 4 parts by mass; Binder: 1 to 1.5 parts by mass.

2. The antibacterial compound feed additive for strengthening fish physique according to claim 1, characterized in that: The preparation method of the alkali-modified attapulgite comprises the following steps: The alkali-modified attapulgite is obtained by pouring the attapulgite into an alkali solution for ultrasonic dispersion, filtering, washing, filtering and drying.

3. The antibacterial compound feed additive for strengthening fish physique according to claim 2, characterized in that: The alkali solution is one or more of a sodium hydroxide solution and a potassium hydroxide solution.

4. The antibacterial compound feed additive for strengthening fish physique according to claim 3, characterized in that: The concentration of the sodium hydroxide solution or potassium hydroxide solution is 2.3 mol / L.

5. The antibacterial compound feed additive for strengthening fish physique according to claim 2, characterized in that: The ultrasonic frequency is 20kHz, the ultrasonic dispersion time is 40-60min, and the ultrasonic dispersion temperature is 70-75°C.

6. The antibacterial compound feed additive for strengthening fish physique according to claim 1, characterized in that: The preparation method of the fermentation product of dandelion and white peony root comprises the following steps: Step 1, weighing dandelion and white peony root in a mass ratio of 2:5, washing, drying, crushing, and mixing to obtain a first mixed material; Step 2, dissolving the low-temperature refrigerated bacterial agent, inoculating it on the culture medium for cultivation and activation, and obtaining an activated bacterial strain; diluting the activated bacterial strain and inoculating it on the first mixed material, with an inoculation amount of 2.5%, and aerobically fermenting it in a constant temperature environment of 40° C. for 12 to 13 hours; Step 3: After the fermentation is completed, the fermentation product is mashed to obtain a paste of dandelion and white peony root fermentation product.

7. The antibacterial compound feed additive for strengthening fish physique according to claim 6, characterized in that: The bacterial agent is prepared by mixing Lactobacillus delbrueckii subspecies bulgaricus, Bifidobacterium adolescentis and Candida tropicalis in a ratio of 4:2:1 in terms of the number of live bacteria.

8. The antibacterial compound feed additive for strengthening fish physique according to claim 1, characterized in that: The adhesive is soluble starch.

9. The method for preparing the antibacterial compound feed additive for strengthening fish physique according to any one of claims 1 to 8, characterized in that: The following steps are involved: Mix the adhesive with 3 to 5 times water to make an adhesive solution; After the fermentation products of dandelion and white peony root are uniformly mixed with alkali-modified attapulgite, a binding liquid is poured in and the stirring is continued to prepare wet granules, and the wet granules are dried in a fluidized bed to obtain the granular antibacterial compound feed additive for strengthening fish physique.

10. Use of the antibacterial compound feed additive for strengthening fish physique according to any one of claims 1 to 8 in the preparation of a feed product for improving fish physique.

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