A Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora and a preparation method thereof

Abstract

The present invention relates to the field of food processing, specifically to a Chinese herbal probiotic compound alcohol-relief beverage and a preparation method thereof. The Chinese herbal probiotic compound for alcohol-relief, liver protection and intestinal flora repair has the following raw materials: licorice, kudzu root, mint, turmeric, tuckahoe, Hovenia dulcis, Dendrobium officinale and plant lactobacillus. The present invention also provides a Chinese herbal probiotic compound beverage for alcohol-relief, liver protection and intestinal flora repair containing the above-mentioned compound. The present invention uses a composite formula to regulate the Chinese medicinal herbs and compound with probiotics, thereby effectively improving the effect of the Chinese herbal probiotic compound beverage in sobering up and maintaining liver function damage caused by excessive drinking and intestinal flora disorder.

Description

Technical Field

[0001] The invention belongs to the field of functional foods, and specifically relates to a Chinese herbal probiotic compound beverage capable of sobering up, protecting the liver and repairing intestinal flora and a preparation method thereof. Background Art

[0002] Drinking is common in social life, but excessive drinking can cause various damages to the body, such as liver damage and gastrointestinal discomfort. There are many kinds of hangover products on the market, but the effects vary. Although traditional Chinese medicine hangover methods have certain effects, they have problems such as poor taste and inconvenience in taking. Probiotics have certain effects in regulating intestinal flora and promoting metabolism, but the effect of using probiotics alone is limited.

[0003] Liver damage mainly comes from inflammatory factor damage caused by slow metabolism, and gastrointestinal discomfort mainly comes from the destruction of intestinal flora by large amounts of ethanol. Protecting the stability of the liver and intestinal flora becomes the most important link before and after drinking.

[0004] As traditional Chinese medicine, some Chinese herbal medicine ingredients have the potential to promote liver detoxification and relieve hangover symptoms. For example, Pueraria root has the effects of clearing heat, promoting body fluid and quenching thirst. Pharmacological studies have shown that it has the effects of anti-oxidative stress response, reducing the accumulation of inflammatory factors, enhancing insulin sensitivity and reducing insulin resistance. Turmeric has the effects of resisting liver damage, lowering blood pressure and blood lipids, promoting menstruation and relieving pain. Poria has the effects of promoting water and dampness, strengthening the spleen and calming the mind. Licorice has the effects of invigorating qi, detoxifying and harmonizing various medicines, and can treat shortness of breath, abdominal pain, food poisoning, etc.

[0005] The addition of probiotics can further enhance the hangover-removing function of traditional Chinese medicine, and increase the speed of hangover-removal. For example, Lactobacillus plantarum is an anaerobic bacterium (facultative aerobic) that can use ethanol as a carbon source for growth, thereby reducing the absorption of ethanol and relieving the burden on the liver; at the same time, it can secrete indole-3-lactic acid, which has the effect of preventing intestinal cancer. Summary of the invention

[0006] The purpose of the present invention is to provide a Chinese herbal probiotic compound beverage and a preparation method for sobering up, protecting the liver, and repairing intestinal flora. The method belongs to the field of functional foods, and the compound beverage contains multiple ingredients such as licorice, kudzu root, poria, mint, turmeric, Hovenia dulcis, Dendrobium candidum, and plant lactobacillus. Among them, licorice invigorates qi, detoxifies, and harmonizes various medicines; kudzu root clears heat, promotes fluid production, and quenches thirst; poria clears heat, promotes fluid production, and quenches thirst; turmeric resists liver damage, lowers blood pressure and reduces blood lipids; mint evacuates wind-heat, clears the head, relieves throat, clears rashes, and soothes the liver and promotes qi; plant lactobacillus reduces serum cholesterol content and prevents cardiovascular diseases, and maintains the balance of intestinal flora. Seven Chinese herbal medicines and probiotics act together on the liver, lungs, spleen, and intestines; mainly sobering up, protecting the liver, protecting the intestines, promoting fluid production and promoting diuresis; supplemented by clearing heat, lowering blood pressure and reducing blood lipids. To achieve the effect of sobering up and protecting the body. The present invention provides a preparation method for the above-mentioned Chinese herbal probiotic compound beverage for sobering up, protecting the liver, and repairing intestinal flora.

[0007] The Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora provided by the present invention is achieved by the following method: The compound beverage includes the following components: (1) One to ten parts of Chinese herbal medicine liquid; (2) 1-5 parts of probiotic freeze-dried powder; The Chinese herbal medicine liquid comprises the following components: (1) Licorice: 1-5 parts; (2) Pueraria root: 3 to 10 parts; (3) Mint: 1-2 parts; (4) Turmeric: 5 to 10 parts; (5) Poria cocos: 3 to 8 parts; (6) Hovenia dulcis: 5-10 parts; (7) Dendrobium officinale: 2-5 parts; The probiotic freeze-dried powder comprises the following components: (1) Lactobacillus plantarum freeze-dried powder: 1-5 parts; The Chinese herbal medicine liquid is prepared by the following steps: (1) Weighing liquorice, kudzu root, mint, turmeric, tuckahoe, Hovenia dulcis, and Dendrobium officinale, mixing and grinding, and obtaining a mixed Chinese herbal medicine powder; (2) The mixed powder of Chinese herbal medicines is mixed with distilled water and then ultrasonically treated to obtain the Chinese herbal medicine liquid to be decocted; (3) decocting the Chinese herbal medicine liquid to obtain the Chinese herbal medicine liquid; The ultrasonic parameters in step 4 are: power 200W, time 10-15min; The decoction process in step 4 is as follows: temperature 85°C-95°C, time 1-2h, decoction times 2-5 times; The freeze-drying process of the plant lactobacillus freeze-dried powder in step 3 is as follows: (1) Centrifuge the cultured Lactobacillus plantarum at 4000 rpm for 10 min and remove the supernatant; (2) Add an appropriate amount of distilled water to resuspend the precipitate; (3) Repeat steps (1) to (2) three times; (4) Add lyophilization protective agent to the washed precipitate and resuspend it; (5) placing the Lactobacillus plantarum liquid containing the lyophilization protectant in liquid nitrogen for quick freezing; (6) placing the quick-frozen Lactobacillus plantarum liquid into a freeze dryer for freeze drying to obtain Lactobacillus plantarum freeze-dried powder; The freeze-drying protective agent formula in step 7 is: (1) Skimmed milk powder 5%-20%; (2) Vitamin C 0.01%-0.2%; (3) Sucrose 2%-5%; The freeze-drying process parameters in step 7 are: (1) -50℃ 12h 100ubar; (2) -20℃ 6h 100ubar; (3) 0℃ 6h 100ubar; The Chinese herbal medicine liquid is sterilized; The process parameters of the 10 sterilization are: 121° C. 15 min; In summary, the advantages and positive effects of the present invention over the prior art are as follows: (1) Lactobacillus plantarum has the ability to metabolize ethanol and remove acetaldehyde, which can prevent alcohol from being converted into toxic carcinogen acetaldehyde, thereby protecting the liver. In addition, Lactobacillus plantarum has many health benefits: ① It has a certain immune regulation effect; ② It has an inhibitory effect on pathogenic bacteria; ③ It reduces serum cholesterol levels and prevents cardiovascular diseases; ④ It maintains the balance of intestinal flora; ⑤ It promotes the absorption of nutrients; ⑥ It relieves lactose intolerance; ⑦ It inhibits the formation of tumor cells, etc.

[0008] (2) Licorice has the effects of tonifying the spleen and replenishing qi, clearing away heat and detoxifying, removing phlegm and relieving cough, relieving pain, and harmonizing various medicines. It is used for spleen and stomach weakness, fatigue, palpitations and shortness of breath, cough with sputum, acute pain in the abdomen and limbs, carbuncle, and sores, and relieves the toxicity and potency of drugs. "Bielu" records: "Warming the middle and lowering qi, cough caused by injured organs, warming the meridians, benefiting blood and qi, and detoxifying all kinds of drugs." The glycyrrhizic acid, glycyrrhizin, liquiritin, and isoliquiritigenin contained in it have effective anti-gastric ulcer and antitussive effects; the glycyrrhizin contained in it has the effect of regulating the body's immune function; the glycyrrhizin flavonoids contained in it have antibacterial and antiviral effects; and the glycyrrhizin contained in it has the effect of detoxification.

[0009] (3) Poria cocos contains tuckahoe polysaccharide, B-tuckahoe polysaccharide, tuckahoe acid, lecithin and sterols. It has the effects of eliminating toxins and fighting cancer, promoting diuresis and eliminating phlegm, strengthening the spleen and eliminating phlegm, and calming the mind. Tuckahoe polysaccharide can significantly increase the phagocytic rate and phagocytic index of phagocytes, thereby achieving the function of enhancing immunity.

[0010] (4) Turmeric has the effects of promoting blood circulation and qi circulation, promoting menstruation and relieving pain. Turmeric contains curcuminol, curcumin, curcumone, curcumene, etc. Curcuminol and curcumin have the effect of lowering blood lipids; curcumin also has anti-tumor, anti-inflammatory, antiviral, choleretic, and antioxidant effects; curcumone and curcumene have the effect of promoting choleretics.

[0011] (5) Mint has the effects of dispelling wind, dissipating heat, warding off filth and detoxifying. Mint contains menthol, menthone and flavonoids; menthol and menthone have the effects of refreshing and relieving pain; flavonoids have the effect of promoting gallbladder function; (6) Pueraria root has the effects of raising yang and relieving muscles, clearing rashes and stopping diarrhea, and relieving restlessness and fever. Pueraria root contains flavonoids such as soybean glycoside, daidzein, puerarin, and puerarin-7-xyloside; these flavonoids have the effects of improving cerebral circulation and lowering blood pressure.

[0012] (7) Hovenia dulcis has the effect of treating drunkenness, restlessness, and thirst. Hovenia dulcis contains ingredients such as loline and rutin, which have the effect of treating drunkenness, restlessness, and thirst.

[0013] (8) Dendrobium candidum has the effects of nourishing yin, clearing away heat, promoting body fluid and quenching thirst. Dendrobium candidum contains alkaloids such as dendrobine and dendrobine, which have the effects of alleviating the symptoms of heat disease, loss of body fluid, thirst and dry tongue, post-illness fever, stomach disease, and retching.

[0014] The Chinese herbal probiotic compound beverage is a separate beverage, which includes Chinese herbal liquid and probiotic freeze-dried powder, and the Chinese herbal medicine and probiotics are mixed before drinking. Compared with the methods of compound or single probiotic single Chinese herbal medicine on the market, the present invention retains the biological activity of probiotics while greatly increasing the efficacy of Chinese herbal medicine, so that it increases the effect of sobering up, strengthens the liver and maintains the stability of intestinal flora, and can effectively reduce the problems of liver damage and intestinal flora disorder caused by drinking. The present invention provides a Chinese herbal probiotic compound beverage and preparation method for sobering up, protecting the liver, and repairing intestinal flora, which overcomes the problems of limited alcohol sobering ability of plants, gastrointestinal discomfort after drinking, and related compound beverages are inactivated probiotics and cannot play the functions of probiotics, and has important application value. DETAILED DESCRIPTION

[0015] To further illustrate the technical means and effects of the present invention, the present invention is further described below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention.

[0016] It should be noted that the following parts are by weight, and the purchase manufacturers of all raw materials involved in the present invention are exemplary without any special restrictions: In the following embodiments, vitamin C CAS is 50-81-7, and the brand is Aladdin; the plant lactobacillus strain number is CICC 24986, and the purchase manufacturer is China Industrial Microbiological Culture Collection Management Center. The following operations involving filling, probiotics cultivation, freeze drying, packaging, etc. all use aseptic conditions such as aseptic operation, aseptic environment, and aseptic filling.

[0017] Example 1 Cultivation of Lactobacillus plantarum Lactobacillus plantarum solid culture medium (MRS) was prepared for picking single colonies of Lactobacillus plantarum. The formula of Lactobacillus plantarum solid culture medium was as follows: casein peptone (trypsin digestion) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween-80 1.0 g, sodium acetate 5.0 g, ammonium citrate tribasic 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, agar powder 15.0 g, distilled water 1000.0 mL, pH 6.2-6.5, and sterilization at 121℃ for 15 min.

[0018] Pour the sterilized MRS solid culture medium into a culture dish. After solidification, take 5uL of Lactobacillus plantarum numbered CICC 24986 and streak culture on the culture dish at 37°C for 22-24h.

[0019] Lactobacillus plantarum medium (MRS) was prepared for Lactobacillus plantarum culture. The formula of Lactobacillus plantarum solid medium was as follows: casein peptone (trypsin digestion) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween-80 1.0 g, sodium acetate 5.0 g, ammonium citrate tribasic 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, distilled water 1000.0 mL, pH 6.2-6.5, sterilization at 121℃ for 15 min.

[0020] Pick a single colony of Lactobacillus plantarum from a culture dish and place it in a first-level seed solution (5 mL) and culture it at 37°C for 8-16 hours; inoculate the first-level seed solution into a second-level seed solution (20 mL) and culture it at 37°C for 8-16 hours; place the second-level seed solution in a fermentation solution and culture it at 37°C for 20-24 hours to obtain a Lactobacillus plantarum bacterial solution.

[0021] Example 2 Preparation of freeze-dried Lactobacillus plantarum The cultured Lactobacillus plantarum liquid was centrifuged at 4000rpm for 10min to separate the bacteria from the liquid. After centrifugation, the supernatant was removed, the bacteria were retained and resuspended in distilled water for washing; after resuspension, the bacteria were centrifuged and washed again, and the above operation was repeated 3 times. After obtaining a clean bacterial precipitate, a freeze-dried protective agent was prepared: skim milk powder 5%-20%; vitamin C 0.01%-0.2%; sucrose 2%-5%. The prepared freeze-dried protective agent was added to the bacterial precipitate and resuspended; after resuspension, 10uL was aspirated for coating on the culture dish for calculating the bacterial concentration.

[0022] The mixture of the lyoprotectant and Lactobacillus plantarum was quickly frozen in liquid nitrogen for 5 minutes. After the quick freezing was completed, it was placed in a freeze dryer for freeze drying. The freeze drying parameters were: -50°C 12h 100ubar; -20°C 6h 100ubar; 0°C 6h 100ubar.

[0023] After freeze-drying, the single / single serving amount is calculated based on the bacterial concentration in the culture dish, and does not exceed 108 CFU. The freeze-dried powder with a composite bacterial concentration dose is added to a special storage space and filled with nitrogen to prevent moisture, and then sealed to obtain the plant lactobacillus freeze-dried powder.

[0024] Example 3 Preparation of Chinese herbal medicine liquid Weigh one to five parts of licorice, three to ten parts of kudzu root, one to two parts of mint, five to ten parts of turmeric, three to eight parts of tuckahoe, five to ten parts of Hovenia dulcis, and two to five parts of Dendrobium candidum, wash them three times with distilled water to remove surface impurities, mix them after washing, and grind them into powder using a grinder to obtain a Chinese herbal medicine mixed powder; add distilled water to the mixed powder, decoct for 1-2 hours at 85℃-95℃, and decoct for 2-5 times; mix and filter the decoction to obtain a transparent Chinese herbal medicine liquid. Sterilize the Chinese herbal medicine liquid at 121℃ for 15 minutes and then fill it.

[0025] Example 4 Comparative Experiment A follow-up survey was conducted on users who took Example 2, Example 3, Example 2+Example 3, and the existing alcohol-relieving botanical beverages on the market [a total of 2 types: Competitor 1 (Chinese herbal probiotic (inactivated) plant compound beverage), Competitor 2 (Chinese herbal botanical beverage)] after drinking. A total of 100 users (80 males and 20 females) were divided into 5 groups, each with 20 people (16 males and 4 females), and used Example 2, Example 3, Example 2+Example 3, Competitor 1, and Competitor 2 respectively. The effect evaluation standard was 10 as the full score, and the average score of each group was taken. The data obtained are as follows:

[0026] Conclusion of comparative experiment: comparing the use of only Example 2, only Example 3, competitor 1, and competitor 2: taking only the active probiotics of Example 2 has a certain ability to sober up, but the efficiency is limited; taking only the Chinese herbal medicine liquid of Example 3 has a high-intensity ability to sober up, but the intestinal microbial flora is more likely to be unbalanced after drinking; both competitor 1 and competitor 2 have strong ability to sober up, but have no obvious effect in maintaining the intestinal microbial flora, which can be basically judged as an imbalance of the intestinal microbial flora after drinking; and the composite plant beverage of Example 2 + Example 3 of the present invention can effectively improve its function of sobering up and protecting the liver, and the function of maintaining the intestinal microbial population, and the degree of sobriety after drinking can directly affect its sobering up function and the degree of protection of liver function; gastrointestinal discomfort after drinking is mostly caused by the imbalance of intestinal microbial flora leading to intestinal dysfunction. This table can reflect the relevant functions of the product in maintaining the intestinal flora, which can effectively avoid intestinal dysfunction.

Claims

1. A Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora and a preparation method thereof, characterized in that: The compound beverage comprises the following components: Chinese herbal medicine liquid: 1-10 parts; Probiotic freeze-dried powder: 1-5 servings; Wherein; the Chinese herbal medicine liquid comprises the following components: Licorice: 1-5 parts; Pueraria root: 3-10 parts; Mint: 1-2 servings; Turmeric: 5-10 portions; Poria: 3-8 parts; Hovenia dulcis: 5-10 parts; Dendrobium officinale: 2-5 parts; The probiotic freeze-dried powder comprises the following components: 1-5 portions of Lactobacillus plantarum freeze-dried powder.

2. The Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora and the preparation method thereof according to claim 1, characterized in that: The Chinese herbal medicine liquid is prepared by the following steps: S101: weighing liquorice, kudzu root, mint, turmeric, tuckahoe, hovenia dulcis, and dendrobium officinale, mixing and grinding to obtain a Chinese herbal medicine mixed powder; S102: mixing the Chinese herbal medicine mixed powder with distilled water and subjecting the mixture to ultrasonic treatment to obtain a Chinese herbal medicine liquid to be decocted; S103: decoct the Chinese herbal medicine liquid to obtain the Chinese herbal medicine liquid.

3. The Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora and the preparation method thereof as claimed in claim 2, characterized in that: The ultrasonic parameters in S102 are power 200W and time 10-15min.

4. The Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora and the preparation method thereof as claimed in claim 2, characterized in that: The decoction process in S103 is performed at a temperature of 85° C.-95° C., for 1-2 hours, and for 2-5 times.

5. The Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora and the preparation method thereof according to claim 1, characterized in that: The freeze-drying process of Lactobacillus plantarum freeze-dried powder is: S201: centrifuging the cultured Lactobacillus plantarum at 4000 rpm for 10 min, and removing the supernatant; S202: adding an appropriate amount of distilled water to the precipitate to resuspend the precipitate; S203: Repeat step (1) to step (2) three times; S204: adding a lyophilization protective agent to the washed precipitate for resuspending; S205: placing the Lactobacillus plantarum liquid containing the lyophilization protectant into liquid nitrogen for quick freezing; S206: placing the quick-frozen Lactobacillus plantarum liquid into a freeze dryer for freeze drying to obtain freeze-dried Lactobacillus plantarum powder.

6. The Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora and the preparation method thereof according to claim 5, characterized in that: The freeze-drying protective agent formula composition in step S204 is: Skim milk powder: 5%-20%; Vitamin C: 0.01%-0.2%; Sucrose: 2%-5%.

7. The Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora and the preparation method thereof according to claim 1, characterized in that: The freeze-drying process parameters in step S206 are: (1) -50℃; 12h; 100ubar; (2) -20℃; 6h; 100ubar; (3) 0℃; 6h; 100ubar.

8. The Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora and the preparation method thereof according to claim 1, characterized in that: The Chinese herbal medicine liquid is sterilized.

9. The Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora and the preparation method thereof according to claim 1, characterized in that: The sterilization process parameters are: 121° C., 15 min.

10. The Chinese herbal probiotic compound beverage for sobering up, protecting the liver and repairing intestinal flora and the preparation method thereof according to claim 1, characterized in that: The Chinese herbal medicine and probiotics compound beverage is a separate beverage, which comprises Chinese herbal medicine liquid and probiotics freeze-dried powder, and the Chinese herbal medicine and probiotics are mixed before drinking.

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