Composition capable of improving water retention and water locking capacity of skin and preparation method of composition

Abstract

The invention relates to the technical field of health-care food, in particular to a composition capable of improving water retention and water locking capacity of skin and a preparation method of the composition. Comprising the following steps: preparing starch salmon nasal cartilage composite nano powder; preparing flavor moisturizing tremella polysaccharide fermentation liquor; and light enzymolysis of the salmon nasal cartilage and compounding of the salmon nasal cartilage and the tremella polysaccharide fermentation liquor. According to the invention, the lactobacillus kefiranofaciens kluyveromyces marxianus subsp. Kefiranofaciens ZW3 bacterial liquid, the kluyveromyces marxianus bacterial liquid and the bacillus subtilis DE111 bacterial liquid which are activated and propagated according to a certain proportion are mixed to prepare the compound bacterial liquid, and then the tremella fuciformis extracting solution is fermented, so that the flavor and taste of the tremella fuciformis extracting solution can be improved, the fermentation effect can be enhanced, and the quality of the tremella fuciformis is improved. Healthy nutritional ingredients such as microbial protein, vitamins and amino acid can be efficiently generated, the antioxidant capacity is enhanced, and the prepared flavored moisturizing tremella polysaccharide fermentation liquor is rich in nutritional ingredients and can improve the antioxidant capacity and the water retention and moisturizing capacity of human skin after being taken into a human body.

Description

Technical Field

[0001] The invention relates to the technical field of health food, and in particular to a composition capable of improving the water-retaining and water-locking ability of skin and a preparation method thereof. Background Art

[0002] Skin moisturizing is an important part of skin care. It can help maintain the moisture balance of the skin and avoid problems such as dryness, roughness, and peeling. With the accelerated pace of modern life and the intensification of environmental pollution, more and more people are facing problems such as dry skin, dehydration, and aging. Dehydrated skin can easily lead to roughness, dullness, loss of elasticity, and wrinkles. Therefore, retaining moisture and locking in moisture is very important for skin health.

[0003] Currently, the main means of moisturizing the skin is through applying or using skin care products, that is, maintaining skin moisture through physical barriers or surface ingredients. In this moisturizing method, the moisture locking layer formed by skin care products is not only easily destroyed with changes in the external environment (such as dry air, temperature changes, etc.), but many moisturizing ingredients (such as glycerin, hyaluronic acid, etc.) can provide surface moisturizing, but cannot effectively penetrate the different layers of the skin and cannot penetrate deep into the skin for long-term effects. In addition, many skin care products contain some potential irritating ingredients, such as alcohol, fragrances, preservatives, etc. These ingredients can extend the shelf life of the product and improve the user experience to a certain extent, but for people with sensitive skin or allergic constitution, they may cause skin discomfort, allergies or irritation reactions. Summary of the invention

[0004] In order to solve the above technical defects, the present invention has developed a method for preparing a composition that can improve the skin's ability to retain water and lock moisture. The composition is prepared by preparing a composite bacterial liquid fermented tremella extract and a high-stability zinc-containing nano-level salmon nasal cartilage powder for compounding. The prepared composition can effectively improve the skin's antioxidant capacity, and can effectively improve the water retention and lock moisture capacity while increasing the moisture content of the muscle skin.

[0005] A method for preparing a composition capable of improving the water-retaining and water-locking ability of the skin comprises the following steps: S1: Preparation of starch-salmon nasal cartilage composite nanopowder The salmon nasal cartilage is blanched, cleaned, and then crushed, and then allowed to stand in an active dry yeast solution, and then washed and dried to obtain the deodorized salmon nasal cartilage, and the deodorized salmon nasal cartilage is sequentially mixed and stirred with a disodium ethylenediaminetetraacetic acid solution and an isopropanol solution, and then washed, dried, and crushed, and then mixed with food-grade zinc sulfate and corn starch for extrusion and expansion, and crushed to obtain starch salmon nasal cartilage composite nanopowder; S2: Preparation of flavored and moisturizing Tremella polysaccharide fermentation broth The Tremella is washed and crushed, and then placed in a constant temperature water bath for hot water extraction, and after repeating once, the filtrate is combined and concentrated to obtain a Tremella extract, and a bacterial solution of Lactobacillus kumiss subsp. kumiss ZW3, a bacterial solution of Kluyveromyces marxianus, and a bacterial solution of Bacillus subtilis DE111 are activated and expanded, and then mixed to obtain a composite bacterial solution, and fructose and ammonium citrate are added to the Tremella extract, and then the composite bacterial solution is added for fermentation to obtain a flavor and moisturizing Tremella polysaccharide fermentation solution; S3: Light enzymatic hydrolysis of salmon nasal cartilage combined with Tremella fuciformis polysaccharide fermentation broth The starch salmon nasal cartilage composite powder is enzymatically hydrolyzed by trypsin and flavor protease to obtain a light enzymatic hydrolyzate of salmon nasal cartilage powder; the flavor moisturizing Tremella polysaccharide fermentation liquid, the light enzymatic hydrolyzate of salmon nasal cartilage powder, maltodextrin, Prunus guanshanensis extract and low-methoxyl pectin are evenly mixed and vacuum freeze-dried to obtain a composition that can improve the skin's water retention and water locking ability.

[0006] Furthermore, step S1 prepares the starch salmon nasal cartilage composite nanopowder, comprising the following steps: S1.1: The fish meat on the surface of the salmon nasal cartilage is shaved with a knife and cut into small pieces, then blanched in boiling water for 4-6 minutes, rinsed with distilled water 2-3 times, and then crushed in a grinder, passed through a 40-45 mesh sieve to obtain primary crushed salmon nasal cartilage, the primary crushed salmon nasal cartilage and deionized water are placed in a container at a solid-liquid ratio of 1: (10-12) g / mL, 3-5wt% of active dry yeast is added and stirred evenly, and then allowed to stand at 40-45°C for 2.5-3 hours, filtered, and the obtained solid matter is washed with distilled water 2-3 times, and then placed in an oven at 60-65°C for drying to obtain deodorized salmon nasal cartilage; S1.2: 1-1.5 parts by weight of disodium ethylenediaminetetraacetate is placed in a container, 15-20 parts by weight of deionized water is added, and then magnetic stirring is performed at a stirring speed of 600-800 rpm until the disodium ethylenediaminetetraacetate is completely dissolved, and then high-pressure sterilization is performed at 121-125°C to obtain a disodium ethylenediaminetetraacetate solution, and the deodorized salmon nasal cartilage is added to the disodium ethylenediaminetetraacetate solution at a solid-liquid ratio of 1: (5-6) g / mL, and the mixture is continuously stirred for 18-20 hours, and then filtered, and then added to a 15-20% isopropanol solution at a solid-liquid ratio of 1: (8-10) g / mL, and the mixture is continuously stirred for 20-24 hours, and then rinsed with distilled water 3-4 times, and then placed in an oven for drying at 60-65°C, and crushed through a 100-120 mesh sieve to obtain pretreated salmon nasal cartilage; S1.3: Place the pretreated salmon nasal cartilage, food-grade zinc sulfate and corn starch in a mass ratio of 1: (0.04-0.08): (2-2.5) in a high-speed mixer, stir at a speed of 1200-1500 rpm for 10-15 minutes, then add to a single-screw extruder, extrude and expand at a speed of 400-450 rpm at 125-130°C, and then crush to obtain starch salmon nasal cartilage composite nanopowder.

[0007] Furthermore, step S2, the preparation of flavored and moisturizing Tremella polysaccharide fermentation liquid, comprises the following steps: S2.1: Rinse the Tremella fuciformis in clean water, cut it into strips, and then place it in a high-speed universal grinder for full crushing, the crushed particle size is 0.1-1mm, and Tremella fuciformis powder is obtained. The Tremella fuciformis powder is mixed evenly with distilled water at a solid-liquid ratio of 1: (25-30) g / mL, and then placed in a constant temperature water bath, heated and stirred at 80-85°C for 2-3 hours, and then filtered to obtain filtrate I and filter residue. The filter residue and distilled water are mixed evenly at a solid-liquid ratio of 1: (15-20) g / mL, heated and stirred in a constant temperature water bath at 80-85°C for a second time for 1-1.5 hours, and then filtered to obtain filtrate II. After mixing filtrate I and filtrate II, the mixture is filtered using gauze with a pore size of 0.2-0.5 mm, and then concentrated in a rotary evaporator at 70-75°C to obtain Tremella fuciformis extract; S2.2: The stored Lactobacillus kumiss subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution were placed at 30-35°C for 1-2 hours, and then the Lactobacillus kumiss subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution were transferred to MRS medium, PDA medium and beef extract peptone medium for activation culture for 12-15 hours under sterile conditions. The volume ratio of the bacterial solution to the culture medium is 1: (450-500), and then the obtained activated bacterial solution is inoculated into the corresponding culture medium at an inoculum amount of 4-5% for 24-30 hours of expansion culture, and the expanded cultured Lactobacillus kumiss subsp. kumiss ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution are mixed and shaken evenly at a volume ratio of 1: (0.6-0.8): (0.3-0.4) to obtain a composite bacterial solution; S2.3: Place the Tremella extract in a fermentation container, add 4-5wt% fructose and 6-6.5wt% ammonium citrate, and then sterilize at 121-125℃ for 25-30 minutes. After cooling, inoculate the composite bacterial liquid prepared in step S2.2 at an inoculum amount of 10-12%, place it at a temperature of 30-35℃ and a rotation speed of 120-150rpm for 40-48 hours, and then centrifuge it at a speed of 8000-10000rpm, filter it to obtain the supernatant, place it in a water bath at 65-70℃ and heat it for 30-35 minutes to obtain the flavor and moisturizing Tremella polysaccharide fermentation liquid, and store it at 3-4℃.

[0008] Furthermore, step S3 of light enzymatic hydrolysis of salmon nasal cartilage and compounding with Tremella polysaccharide fermentation liquid comprises the following steps: S3.1: Place the starch salmon nasal cartilage composite powder and distilled water in a container at a material-liquid ratio of 1: (8-10) g / mL, adjust the pH to 8.5-9, heat to 48-50°C, add trypsin and flavor protease, wherein the enzyme dosage of trypsin is 3500-4000 U / g, and the enzyme dosage of flavor protease is 2500-3000 U / g, and after enzymolysis for 1.5-2 hours, heat at 95-100°C for 10-15 minutes to obtain a light enzymatic hydrolyzate of salmon nasal cartilage powder; S3.2: Take flavored moisturizing Tremella polysaccharide fermentation liquid, and add 85-95% of salmon nasal cartilage powder light enzymatic hydrolyzate, 10-15% of maltodextrin, 2-5% of Cherry blossom extract, 1-5% of low-methoxyl pectin, 0.1-0.3% of niacinamide and 1.5-2% of erythritol, based on the mass percentage of the flavored moisturizing Tremella polysaccharide fermentation liquid, mix well, and then vacuum freeze-dry at -60°C to -40°C to obtain a composition that can improve the skin's ability to retain water.

[0009] Furthermore, in step S1.1, the salmon nasal cartilage is cut into small pieces, and the particle size of the small pieces is 3-5 cm.

[0010] Furthermore, in step S1.2, when the deodorized salmon nasal cartilage is added to the disodium ethylenediaminetetraacetic acid solution and the isopropanol solution, the temperature is maintained at 3-4°C.

[0011] Furthermore, in step S2.1, the filtrate I and the filtrate II are mixed and filtered, and then concentrated by rotary evaporation, and the volume after concentration is 1 / 8-1 / 7 of the original volume.

[0012] Furthermore, in step S2.2, the activation and expansion culture temperature of the Lactobacillus kruskalensis subsp. kruskalensis ZW3 bacterial solution and the Kluyveromyces marxianus bacterial solution were both 30°C, and the activation and expansion culture temperature of the Bacillus subtilis DE111 bacterial solution was 37°C.

[0013] Furthermore, in step S3.1, the pH is adjusted by adding a sodium hydroxide solution having a concentration of 0.1-0.15 mol / L.

[0014] A composition capable of improving the water-retaining and water-locking ability of the skin is prepared by the preparation method of the composition capable of improving the water-retaining and water-locking ability of the skin.

[0015] The beneficial effects are as follows: 1. The present invention extracts Tremella fuciformis by hot water to prepare Tremella fuciformis extract, then mixes a certain proportion of activated and expanded Lactobacillus kumiss subspecies ZW3 bacterial liquid, Kluyveromyces marxianus bacterial liquid and Bacillus subtilis DE111 bacterial liquid to prepare a composite bacterial liquid, adds fructose and ammonium citrate to the Tremella fuciformis extract, and then uses the composite bacterial liquid to perform fermentation treatment to prepare a flavor and moisturizing Tremella fuciformis polysaccharide fermentation liquid, the Lactobacillus kumiss subspecies ZW3 bacterial liquid can not only promote the conversion of sugar and protein, generate short-chain fatty acids and esters, etc., which are helpful to improve the flavor and taste of the Tremella fuciformis extract and reduce its fishy and bitter substances, but also can generate ZW3 extracellular polysaccharide in the fermentation process, and the ZW3 extracellular polysaccharide not only has excellent emulsification ability, but also can promote the contact between the extract molecules and the bacterial flora. , and it also has good flocculation ability, which can promote the interaction mechanism of each bacterial community in the composite bacterial liquid in the extract and enhance the fermentation effect. The Kluyveromyces marxianus liquid can use simple inorganic salts and sugars to achieve high-density fermentation, and can also efficiently generate healthy nutrients such as microbial proteins, vitamins and amino acids in the tremella extract. Bacillus subtilis DE111 has a broad-spectrum antibacterial activity and antioxidant ability, and can inhibit harmful bacteria such as Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa and Candida albicans. It can also generate short-chain fatty acids and promote the growth of other probiotics, thereby regulating the balance of intestinal flora. In summary, the prepared flavored and moisturizing tremella polysaccharide fermentation liquid is rich in nutrients, and ingestion can improve the antioxidant capacity and water-retaining capacity of human skin.

[0016] The present invention utilizes trypsin and flavor protease to perform enzymolysis on the starch salmon nasal cartilage composite powder for 1.5-2 hours, so that the peptide components in part of the protein of the salmon nasal cartilage powder can be exposed, so as to be chelated with zinc ions in zinc sulfate to form a highly stable salmon nasal cartilage powder, which can not only prevent the protein from being excessively decomposed, thereby avoiding the proteoglycan formed by covalent bond with the protein from being dissolved in the solution, retaining the protein polysaccharide components unique to the salmon nasal cartilage powder, but also introduce an appropriate amount of zinc ions, which can be added into the flavor moisturizing Tremella polysaccharide fermentation liquid to better regulate the moisture and oil balance of the skin, thereby promoting collagen synthesis and enhancing the skin's water retention and water locking ability.

[0017] The invention removes the fishy smell from the salmon nasal cartilage and grinds it, and then mixes it with food-grade zinc sulfate and corn starch for extrusion expansion, which can not only improve the taste and flavor of the composition, but also the food-grade zinc sulfate and corn starch can further reduce the particle size of the salmon nasal cartilage during the expansion process to obtain the starch-salmon nasal cartilage composite nano powder, thereby improving the utilization rate of the nutrients in the salmon nasal cartilage in the human body, and can better chelate with zinc ions in the subsequent enzymatic hydrolysis process, thereby enhancing the moisture retention ability and stability of the composition that can improve the water retention and water locking ability of the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a flow chart of a method for preparing a composition capable of improving the skin's ability to retain moisture, as used in an embodiment of the present invention. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Embodiment 1: A composition capable of improving the water-retaining and water-locking ability of the skin and a preparation method thereof, such as Figure 1 As shown, the following steps are included: S1: Preparation of starch-salmon nasal cartilage composite nanopowder S1.1: The fish meat on the surface of the salmon nasal cartilage was shaved with a knife and cut into small pieces with a particle size of 3 cm, then blanched in boiling water for 4 minutes, rinsed twice with distilled water, and then crushed in a grinder, and passed through a 40-mesh sieve to obtain primary crushed salmon nasal cartilage, and the primary crushed salmon nasal cartilage and deionized water were placed in a container at a solid-liquid ratio of 1:10 g / mL, 3 wt% of active dry yeast was added and stirred evenly, and then allowed to stand at 40°C for 2.5 hours, and after filtering, the obtained solid matter was washed twice with distilled water, and then placed in an oven at 60°C for drying to obtain deodorized salmon nasal cartilage; S1.2: Place 1 part by weight of disodium ethylenediaminetetraacetate in a container, add 15 parts by weight of deionized water, and stir magnetically at a stirring speed of 600 rpm until the disodium ethylenediaminetetraacetate is completely dissolved, and then sterilize under high pressure at 121°C to obtain a disodium ethylenediaminetetraacetate solution. At 3°C, add the deodorized salmon nasal cartilage to the disodium ethylenediaminetetraacetate solution at a solid-liquid ratio of 1:5 g / mL, continue stirring for 18 hours, filter, and then add it to a 15% isopropanol solution at a solid-liquid ratio of 1:8 g / mL. Continue stirring for 20 hours, then rinse with distilled water 3 times, and then place it in an oven for drying at 60°C, and crush it through a 100-mesh sieve to obtain pretreated salmon nasal cartilage; S1.3: Place the pretreated salmon nasal cartilage, food-grade zinc sulfate and corn starch in a mass ratio of 1:0.04:2 in a high-speed mixer, stir at a speed of 1200 rpm for 10 minutes, then add into a single-screw extruder, extrude and expand at a speed of 400 rpm at 125°C, and then crush to obtain starch salmon nasal cartilage composite nanopowder.

[0021] S2: Preparation of flavored and moisturizing Tremella polysaccharide fermentation broth S2.1: Rinse the Tremella fuciformis in clean water, cut it into strips, and then place it in a high-speed universal grinder for full crushing. The crushed particle size is 0.1 mm to obtain Tremella fuciformis powder. The Tremella fuciformis powder and distilled water are mixed evenly at a solid-liquid ratio of 1:25 g / mL, and then placed in a constant temperature water bath, heated and stirred at 80°C for 2 hours, and then filtered to obtain filtrate I and filter residue. The filter residue and distilled water are mixed evenly at a solid-liquid ratio of 1:15 g / mL, heated and stirred in a constant temperature water bath at 80°C for a second time for 1 hour, and then filtered to obtain filtrate II. After mixing filtrate I and filtrate II, the mixture is filtered using gauze with a pore size of 0.2 mm, and then concentrated in a rotary evaporator at 70°C until the volume is 1 / 8 of the original volume to obtain Tremella fuciformis extract; S2.2: The preserved Klebsiella pneumoniae subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution were placed at 30°C for 1 hour, and then the Klebsiella pneumoniae subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution were respectively transferred to MRS medium, PDA medium and beef extract peptone medium for activation culture for 12 hours under sterile conditions, and the volume ratio of the bacterial solution to the culture medium was 1:450, and then the obtained activated bacterial solution was inoculated into the corresponding culture medium at an inoculum amount of 4% for 24 hours of expansion culture, and the Klebsiella pneumoniae subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution after expansion culture were mixed and shaken evenly at a volume ratio of 1:0.6:0.3 to obtain a composite bacterial solution; S2.3: Place the Tremella extract in a fermentation container, add 4wt% fructose and 6wt% ammonium citrate, and then sterilize at 121°C for 25 minutes. After cooling, inoculate the composite bacterial liquid prepared in step S2.2 at a 10% inoculation rate, place it at 30°C and shake culture at 120rpm for 40 hours, then centrifuge it at 8000rpm, filter it to obtain the supernatant, place it in a water bath at 65°C and heat it for 30 minutes to obtain the flavor and moisturizing Tremella polysaccharide fermentation liquid, and store it at 3°C.

[0022] S3: Light enzymatic hydrolysis of salmon nasal cartilage combined with Tremella fuciformis polysaccharide fermentation broth S3.1: The starch salmon nasal cartilage composite powder and distilled water are placed in a container at a material-liquid ratio of 1:8 g / mL, and the pH is adjusted to 8.5 with a sodium hydroxide solution having a concentration of 0.1 mol / L. After heating to 48°C, trypsin and flavor protease are added, wherein the enzyme dosage of trypsin is 3500 U / g and the enzyme dosage of flavor protease is 2500 U / g. After enzymolysis for 1.5 hours, the mixture is heated at 95°C for 10 minutes to obtain a light enzymatic hydrolyzate of salmon nasal cartilage powder; S3.2: Take flavored moisturizing Tremella polysaccharide fermentation liquid, and add 85% of salmon nasal cartilage powder light enzymatic hydrolysate, 10% of maltodextrin, 2% of Cherry blossom extract, 1% of low-methoxyl pectin, 0.1% of niacinamide and 1.5% of erythritol, based on the mass percentage of the flavored moisturizing Tremella polysaccharide fermentation liquid, mix well, and then vacuum freeze-dry at minus 60°C to obtain a composition that can improve the skin's ability to retain water.

[0023] Embodiment 2: A composition capable of improving the water-retaining and water-locking ability of the skin and a preparation method thereof, such as Figure 1 As shown, the following steps are included: S1: Preparation of starch-salmon nasal cartilage composite nanopowder S1.1: The fish meat on the surface of the salmon nasal cartilage was shaved with a knife and cut into small pieces with a particle size of 3 cm, then blanched in boiling water for 4 minutes, rinsed twice with distilled water, and then crushed in a grinder, and passed through a 40-mesh sieve to obtain primary crushed salmon nasal cartilage, and the primary crushed salmon nasal cartilage and deionized water were placed in a container at a solid-liquid ratio of 1:12 g / mL, 5 wt% active dry yeast was added and stirred evenly, and then allowed to stand at 40°C for 2.5 hours, filtered, and the obtained solid matter was washed twice with distilled water, and then placed in an oven at 60°C for drying to obtain deodorized salmon nasal cartilage; S1.2: 1.5 parts by weight of disodium ethylenediaminetetraacetate was placed in a container, 20 parts by weight of deionized water was added, and the mixture was magnetically stirred at a stirring speed of 600 rpm until the disodium ethylenediaminetetraacetate was completely dissolved, and then sterilized under high pressure at 121°C to obtain a disodium ethylenediaminetetraacetate solution. At 3°C, the deodorized salmon nasal cartilage was added to the disodium ethylenediaminetetraacetate solution at a solid-liquid ratio of 1:6 g / mL, and the mixture was continuously stirred for 18 hours, and then filtered. The mixture was then added to a 15% isopropanol solution at a solid-liquid ratio of 1:10 g / mL, and the mixture was continuously stirred for 20 hours, and then rinsed with distilled water for 3 times, and then dried in an oven at 60°C, and crushed through a 100-mesh sieve to obtain pretreated salmon nasal cartilage. S1.3: Place the pretreated salmon nasal cartilage, food-grade zinc sulfate and corn starch in a mass ratio of 1:0.08:2.5 in a high-speed mixer, stir at a speed of 1200 rpm for 10 minutes, then add into a single-screw extruder, extrude and expand at a speed of 400 rpm at 125°C, and then crush to obtain starch salmon nasal cartilage composite nanopowder.

[0024] S2: Preparation of flavored and moisturizing Tremella polysaccharide fermentation broth S2.1: Rinse the Tremella fuciformis in clean water, cut it into strips, and then place it in a high-speed universal grinder for full crushing, the crushed particle size is 0.1 mm, and Tremella fuciformis powder is obtained. The Tremella fuciformis powder is mixed evenly with distilled water at a solid-liquid ratio of 1:30 g / mL, and then placed in a constant temperature water bath, heated and stirred at 80°C for 2 hours, and then filtered to obtain filtrate I and filter residue. The filter residue and distilled water are mixed evenly at a solid-liquid ratio of 1:20 g / mL, heated and stirred in a constant temperature water bath at 80°C for a second time for 1 hour, and then filtered to obtain filtrate II. After mixing filtrate I and filtrate II, the mixture is filtered using gauze with a pore size of 0.2 mm, and then concentrated in a rotary evaporator at 70°C until the volume is 1 / 8 of the original volume, to obtain Tremella fuciformis extract; S2.2: The preserved Klebsiella pneumoniae subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution were placed at 30°C for 1 hour, and then the Klebsiella pneumoniae subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution were respectively transferred to MRS medium, PDA medium and beef extract peptone medium for activation culture for 12 hours under sterile conditions, and the volume ratio of the bacterial solution to the medium was 1:450, and then the obtained activated bacterial solution was inoculated into the corresponding culture medium at an inoculum amount of 4% for 24 hours of expansion culture, and the Klebsiella pneumoniae subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution after expansion culture were mixed and shaken evenly at a volume ratio of 1:0.8:0.4 to obtain a composite bacterial solution; S2.3: Place the Tremella extract in a fermentation container, add 5wt% fructose and 6.5wt% ammonium citrate, and then sterilize at 121°C for 25 minutes. After cooling, inoculate the composite bacterial liquid prepared in step S2.2 at a rate of 12%, place it at 30°C and shake it at 120rpm for 40 hours, then centrifuge it at 8000rpm, filter it to obtain the supernatant, place it in a water bath at 65°C and heat it for 30 minutes to obtain the flavored and moisturizing Tremella polysaccharide fermentation liquid, and store it at 3°C.

[0025] S3: Light enzymatic hydrolysis of salmon nasal cartilage combined with Tremella fuciformis polysaccharide fermentation broth S3.1: The starch salmon nasal cartilage composite powder and distilled water are placed in a container at a material-liquid ratio of 1:10 g / mL, and the pH is adjusted to 8.5 with a sodium hydroxide solution having a concentration of 0.1 mol / L. After heating to 48°C, trypsin and flavor protease are added, wherein the enzyme dosage of trypsin is 3500 U / g and the enzyme dosage of flavor protease is 2500 U / g. After enzymolysis for 1.5 hours, the mixture is heated at 95°C for 10 minutes to obtain a light enzymatic hydrolyzate of salmon nasal cartilage powder; S3.2: Take flavored moisturizing Tremella polysaccharide fermentation liquid, and add 95% of salmon nasal cartilage powder light enzymatic hydrolyzate, 15% of maltodextrin, 5% of Cherry blossom extract, 5% of low-methoxyl pectin, 0.3% of niacinamide and 2% of erythritol, based on the mass percentage of the flavored moisturizing Tremella polysaccharide fermentation liquid, mix well, and then vacuum freeze-dry at minus 60°C to obtain a composition that can improve the skin's ability to retain water.

[0026] Embodiment 3: A composition capable of improving the water-retaining and water-locking ability of the skin and a preparation method thereof, such as Figure 1 As shown, the following steps are included: S1: Preparation of starch-salmon nasal cartilage composite nanopowder S1.1: The fish meat on the surface of the salmon nasal cartilage was shaved with a knife and cut into small pieces with a particle size of 5 cm, then blanched in boiling water for 6 minutes, rinsed with distilled water for 3 times, and then crushed in a grinder, and passed through a 45-mesh sieve to obtain primary crushed salmon nasal cartilage, and the primary crushed salmon nasal cartilage and deionized water were placed in a container at a solid-liquid ratio of 1:12 g / mL, 3 wt% active dry yeast was added and stirred evenly, and then allowed to stand at 45°C for 3 hours, and after filtering, the obtained solid matter was washed with distilled water for 3 times, and then placed in an oven at 65°C for drying to obtain deodorized salmon nasal cartilage; S1.2: Place 1 part by weight of disodium ethylenediaminetetraacetate in a container, add 15 parts by weight of deionized water, and stir magnetically at a stirring speed of 800 rpm until the disodium ethylenediaminetetraacetate is completely dissolved, and then sterilize under high pressure at 125°C to obtain a disodium ethylenediaminetetraacetate solution. At 4°C, add the deodorized salmon nasal cartilage to the disodium ethylenediaminetetraacetate solution at a solid-liquid ratio of 1:5 g / mL, continue stirring for 20 hours, filter, and then add it to a 20% isopropanol solution at a solid-liquid ratio of 1:8 g / mL. Continue stirring for 24 hours, rinse with distilled water 4 times, and then place it in an oven for drying at 65°C, and crush it through a 120-mesh sieve to obtain pretreated salmon nasal cartilage; S1.3: Place the pretreated salmon nasal cartilage, food-grade zinc sulfate and corn starch in a mass ratio of 1:0.04:2 in a high-speed mixer, stir at a speed of 1500 rpm for 15 minutes, then add into a single-screw extruder, extrude and expand at a speed of 450 rpm at 130°C, and then crush to obtain starch salmon nasal cartilage composite nanopowder.

[0027] S2: Preparation of flavored and moisturizing Tremella polysaccharide fermentation broth S2.1: Rinse the Tremella fuciformis in clean water, cut it into strips, and then place it in a high-speed universal grinder for full crushing. The crushed particle size is 1 mm to obtain Tremella fuciformis powder. The Tremella fuciformis powder and distilled water are mixed evenly at a solid-liquid ratio of 1:25 g / mL, and then placed in a constant temperature water bath, heated and stirred at 85°C for 3 hours, and then filtered to obtain filtrate I and filter residue. The filter residue and distilled water are mixed evenly at a solid-liquid ratio of 1:15 g / mL, heated and stirred in a constant temperature water bath at 85°C for a second time for 1.5 hours, and then filtered to obtain filtrate II. After mixing filtrate I and filtrate II, the mixture is filtered using gauze with a pore size of 0.5 mm, and then concentrated in a rotary evaporator at 75°C until the volume is 1 / 7 of the original volume to obtain Tremella fuciformis extract; S2.2: The preserved Lactobacillus kühm subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution were placed at 35°C for 2 hours, and then the Lactobacillus kühm subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution were respectively transferred to MRS medium, PDA medium and beef extract peptone medium for 15 hours of activation culture under sterile conditions, and the volume ratio of the bacterial solution to the culture medium was 1:500, and then the obtained activated bacterial solution was inoculated into the corresponding culture medium at an inoculation amount of 4% for 30 hours of expansion culture, and the Lactobacillus kühm subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution after expansion culture were mixed and shaken evenly at a volume ratio of 1:0.6:0.3 to obtain a composite bacterial solution; S2.3: Place the Tremella extract in a fermentation container, add 4wt% fructose and 6wt% ammonium citrate, and then sterilize at 125℃ for 30 minutes. After cooling, inoculate the composite bacterial liquid prepared in step S2.2 at a 10% inoculation rate, place it at 35℃ and shake culture at 150rpm for 48 hours, then centrifuge it at 1000rpm, filter it to obtain the supernatant, place it in a 70℃ water bath and heat it for 35 minutes to obtain the flavor and moisturizing Tremella polysaccharide fermentation liquid, and store it at 4℃.

[0028] S3: Light enzymatic hydrolysis of salmon nasal cartilage combined with Tremella fuciformis polysaccharide fermentation broth S3.1: The starch salmon nasal cartilage composite powder and distilled water are placed in a container at a material-liquid ratio of 1:8 g / mL, and the pH is adjusted to 9 with a sodium hydroxide solution having a concentration of 0.1 mol / L. After heating to 50°C, trypsin and flavor protease are added, wherein the enzyme dosage of trypsin is 4000 U / g and the enzyme dosage of flavor protease is 3000 U / g. After enzymolysis for 2 hours, the mixture is heated at 100°C for 15 minutes to obtain a light enzymatic hydrolyzate of salmon nasal cartilage powder; S3.2: Take flavored moisturizing Tremella polysaccharide fermentation liquid, and add 85% of salmon nasal cartilage powder light enzymatic hydrolyzate, 10% of maltodextrin, 2% of Cherry blossom extract, 1% of low-methoxyl pectin, 0.1% of niacinamide and 1.5% of erythritol, based on the mass percentage of the flavored moisturizing Tremella polysaccharide fermentation liquid, mix well, and then vacuum freeze-dry at -40°C to obtain a composition that can improve the skin's ability to retain water.

[0029] Comparative Example 1 The difference from Example 1 is that, in Comparative Example 1, the kumiss-like Lactobacillus kumiss subspecies ZW3 bacterial liquid is not activated and expanded in step S2.2, and the kumiss-like Lactobacillus kumiss subspecies ZW3 bacterial liquid expanded and cultured in the composite bacterial liquid is replaced with an equal mass of activated and expanded Lactobacillus plantarum, and the remaining steps are the same as in Example 1. The obtained composition that can improve the skin's ability to retain water is recorded as Comparative Example 1.

[0030] Comparative Example 2 The difference from Example 1 is that in Comparative Example 2, the Kluyveromyces marxianus culture solution is not activated and expanded in step S2.2, and the Kluyveromyces marxianus culture solution after expansion and culture in the composite culture solution is replaced with an equal mass of activated and expanded Saccharomyces cerevisiae, and the remaining steps are the same as those in Example 1. The obtained composition that can improve the skin's water retention and water locking ability is recorded as Comparative Example 2.

[0031] Comparative Example 3 The difference from Example 1 is that the enzymatic hydrolysis time in step S3.1 of Comparative Example 3 is 1 hour, and a light enzymatic hydrolyzate of salmon nasal cartilage powder is obtained. The remaining steps are the same as those in Example 1. The obtained composition that can improve the skin's water retention and water locking ability is recorded as Comparative Example 3.

[0032] Comparative Example 4 The difference from Example 1 is that the enzymatic hydrolysis time in step S3.1 of Comparative Example 4 is 2.5 hours, and a light enzymatic hydrolyzate of salmon nasal cartilage powder is obtained. The remaining steps are the same as those in Example 1. The obtained composition that can improve the skin's water retention and water locking ability is recorded as Comparative Example 4.

[0033] Comparative Example 5 The difference from Example 1 is that, in Comparative Example 5, step S1.3 is removed, and the starch salmon nasal cartilage composite powder in step S3.1 is replaced with an equal mass of pretreated salmon nasal cartilage, and the remaining steps are the same as in Example 1. The obtained composition that can improve the skin's ability to retain water is recorded as Comparative Example 5.

[0034] Comparative Example 6 The difference from Example 1 is that in Comparative Example 6, food-grade zinc sulfate is not added in step S3.1 to obtain starch salmon nasal cartilage composite nanopowder, and the remaining steps are the same as Example 1. The obtained composition that can improve the skin's water retention and water locking ability is recorded as Comparative Example 6.

[0035] Experiment 1: Take equal masses of the composition for improving the water retention and water locking ability of the skin prepared in Examples 1-3 and Comparative Examples 1-2, respectively, and mix them with deionized water to prepare a sample solution with a concentration of 10 mg / mL, dissolve DPPH in anhydrous ethanol to prepare a 0.2 mmol / L DPHH solution, mix 2 mL of DPHH solution with 2 mL of anhydrous ethanol to prepare a mixed solution, recorded as C0, mix 2 mL of the sample solution with 2 mL of anhydrous ethanol to prepare a mixed solution, recorded as C1, and mix 2 mL of the sample solution with 2 mL of DPHH solution to prepare a mixed solution, recorded as C2, DPPH free radical scavenging rate = (1-(C1-C2) / C0) × 100%, record the data and make a table, repeat the parallel experiment once and record it as the experimental group and the parallel experimental group, as shown in Table 1.

[0036] Table 1: DPPH free radical scavenging rate of compositions capable of improving the skin's ability to retain moisture

[0037] It can be seen from the data of Examples 1-3 in Table 1 that a composite bacterial solution is prepared by mixing the activated and expanded Lactobacillus kumiss subspecies ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution, and then the Tremella extract is fermented. The compositions that can improve the skin's water retention and water locking ability finally obtained all have excellent DPPH radical scavenging ability, and it can be seen from the data of Comparative Examples 1-2 that when the composite bacterial solution is prepared by replacing Lactobacillus kumiss subspecies with Lactobacillus plantarum and Kluyveromyces marxianus with Saccharomyces cerevisiae for fermentation, the DPPH radical scavenging ability of the composition is reduced, proving that there is a synergistic effect between the composite bacterial solutions of the present embodiment, which can greatly enhance the fermentation ability of the Tremella extract, and the compositions that can improve the skin's water retention and water locking ability made from the flavored moisturizing Tremella polysaccharide fermentation solution obtained by fermentation in the embodiment all have excellent antioxidant capacity.

[0038] Experiment 2: Take 5 g of the composition prepared in Examples 1-3 that can improve the skin's water retention and water locking ability and Comparative Examples 1-6, use 25 mL of 37°C warm water to mix, stir evenly to prepare an oral sample, and take 50 SD rats, 25 females and 25 males, weighing 200±20 g, and divide them into 10 groups on average, 5 rats in each group, one group as a blank group fed with ordinary feed, and each of the remaining groups was gavage-fed with one of the oral samples prepared from the composition of Examples 1-3 that can improve the skin's water retention and water locking ability and the oral samples prepared from Comparative Examples 1-6, once a day for 30 days.

[0039] Before feeding on the 1st day, the 11th day, the 21st day and the 31st day, the skin of the same area on the back of each group of rats was carefully cleaned and depilated, and then the moisture content of the depilated and cleaned area of ​​the rat skin was tested using a RealBubeeRBX-916 skin analysis tester. The average moisture content of the skin of each group of rats was calculated, and the skin moisture growth rate = (average moisture content on the nth day - average moisture content on the first day) / average moisture content on the first day was calculated. The data were recorded and tabulated, as shown in Table 2.

[0040] Table 2: Water content in mouse skin before feeding for 11 days, 21 days, and 31 days

[0041] It can be seen from the data in Table 2 that compared with the blank group, the composition prepared in the embodiment for improving the skin's water retention and water locking ability can significantly increase the water content in the skin of SD rats, and from the data of Comparative Examples 1-2, it can be seen that when the kumquat-like Lactobacillus kumquat-like subsp. kumiss is replaced by Lactobacillus plantarum, and the Kluyveromyces marxianus is replaced by Saccharomyces cerevisiae to prepare a composite bacterial liquid for fermentation, the ability of the composition to increase the water content in the skin of SD rats decreases, which can prove that the flavored and moisturizing Tremella polysaccharide fermentation liquid prepared in the embodiment has rich nutrients and can improve the skin's water retention and moisture retention ability when taken into the body.

[0042] It can be seen from the data of Comparative Examples 3-4 in Table 2 that when the time for enzymatic hydrolysis of the starch-salmon nasal cartilage composite powder by trypsin and flavor protease is not within 1.5-2 hours, the water content in the skin of SD rats is reduced compared with that in the embodiment. It can be proved that controlling the time for enzymatic hydrolysis of the starch-salmon nasal cartilage composite powder by trypsin and flavor protease can retain the protein and polysaccharide components unique to the salmon nasal cartilage powder while introducing an appropriate amount of zinc ions, thereby enhancing the skin's ability to retain water.

[0043] It can be seen from the data of Comparative Example 5 in Table 2 that when the pretreated salmon nasal cartilage is mixed with food-grade zinc sulfate and corn starch for extrusion and puffing, the water content in the skin of SD rats is reduced compared with the example. It can be proved that the extrusion and puffing of pretreated salmon nasal cartilage with food-grade zinc sulfate and corn starch can improve the utilization rate of nutrients in salmon nasal cartilage in the human body, thereby enhancing the moisturizing ability of the composition that can improve the skin's water retention and water locking ability.

[0044] It can be seen from the data of Comparative Example 6 in Table 2 that when extrusion and puffing were performed without adding food-grade zinc sulfate, the water content in the skin of SD rats decreased compared with the example, which can prove that the introduction of an appropriate amount of zinc ions can promote collagen synthesis and enhance the skin's ability to retain water.

[0045] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A method for preparing a composition capable of improving the water-retaining and water-locking ability of the skin, characterized in that: The following steps are involved: S1: Preparation of starch-salmon nasal cartilage composite nanopowder The salmon nasal cartilage is blanched, cleaned, and then crushed, and then allowed to stand in an active dry yeast solution, and then washed and dried to obtain the deodorized salmon nasal cartilage, and the deodorized salmon nasal cartilage is sequentially mixed and stirred with a disodium ethylenediaminetetraacetic acid solution and an isopropanol solution, and then washed, dried, and crushed, and then mixed with food-grade zinc sulfate and corn starch for extrusion and expansion, and crushed to obtain starch salmon nasal cartilage composite nanopowder; S2: Preparation of flavored and moisturizing Tremella polysaccharide fermentation broth The Tremella is washed and crushed, and then placed in a constant temperature water bath for hot water extraction, and after repeating once, the filtrate is combined and concentrated to obtain a Tremella extract, and a bacterial solution of Lactobacillus kumiss subsp. kumiss ZW3, a bacterial solution of Kluyveromyces marxianus, and a bacterial solution of Bacillus subtilis DE111 are activated and expanded, and then mixed to obtain a composite bacterial solution, and fructose and ammonium citrate are added to the Tremella extract, and then the composite bacterial solution is added for fermentation to obtain a flavor and moisturizing Tremella polysaccharide fermentation solution; S3: Light enzymatic hydrolysis of salmon nasal cartilage combined with Tremella fuciformis polysaccharide fermentation broth The starch salmon nasal cartilage composite powder is enzymatically hydrolyzed by trypsin and flavor protease to obtain a light enzymatic hydrolyzate of salmon nasal cartilage powder; the flavor moisturizing Tremella polysaccharide fermentation liquid, the light enzymatic hydrolyzate of salmon nasal cartilage powder, maltodextrin, Prunus guanshanensis extract and low-methoxyl pectin are evenly mixed and vacuum freeze-dried to obtain a composition that can improve the skin's water retention and water locking ability.

2. A method for preparing a composition capable of improving the water-retaining and water-locking ability of the skin according to claim 1, characterized in that: Step S1: preparing starch salmon nasal cartilage composite nanopowder, comprising the following steps: S1.1: The fish meat on the surface of the salmon nasal cartilage is shaved with a knife and cut into small pieces, then blanched in boiling water for 4-6 minutes, rinsed with distilled water 2-3 times, and then crushed in a grinder, passed through a 40-45 mesh sieve to obtain primary crushed salmon nasal cartilage, the primary crushed salmon nasal cartilage and deionized water are placed in a container at a solid-liquid ratio of 1: (10-12) g / mL, 3-5wt% of active dry yeast is added and stirred evenly, and then allowed to stand at 40-45°C for 2.5-3 hours, filtered, and the obtained solid matter is washed with distilled water 2-3 times, and then placed in an oven at 60-65°C for drying to obtain deodorized salmon nasal cartilage; S1.2: 1-1.5 parts by weight of disodium ethylenediaminetetraacetate is placed in a container, 15-20 parts by weight of deionized water is added, and then magnetic stirring is performed at a stirring speed of 600-800 rpm until the disodium ethylenediaminetetraacetate is completely dissolved, and then high-pressure sterilization is performed at 121-125°C to obtain a disodium ethylenediaminetetraacetate solution, and the deodorized salmon nasal cartilage is added to the disodium ethylenediaminetetraacetate solution at a solid-liquid ratio of 1: (5-6) g / mL, and the mixture is continuously stirred for 18-20 hours, and then filtered, and then added to a 15-20% isopropanol solution at a solid-liquid ratio of 1: (8-10) g / mL, and the mixture is continuously stirred for 20-24 hours, and then rinsed with distilled water 3-4 times, and then placed in an oven for drying at 60-65°C, and crushed through a 100-120 mesh sieve to obtain pretreated salmon nasal cartilage; S1.3: Place the pretreated salmon nasal cartilage, food-grade zinc sulfate and corn starch in a mass ratio of 1: (0.04-0.08): (2-2.5) in a high-speed mixer, stir at a speed of 1200-1500 rpm for 10-15 minutes, then add to a single-screw extruder, extrude and expand at a speed of 400-450 rpm at 125-130°C, and then crush to obtain starch salmon nasal cartilage composite nanopowder.

3. The method for preparing a composition capable of improving the water-retaining and water-locking ability of the skin according to claim 2, characterized in that: Step S2 is the preparation of flavored and moisturizing Tremella polysaccharide fermentation liquid, comprising the following steps: S2.1: Rinse the Tremella fuciformis in clean water, cut it into strips, and then place it in a high-speed universal grinder for full crushing, the crushed particle size is 0.1-1mm, and Tremella fuciformis powder is obtained. The Tremella fuciformis powder is mixed evenly with distilled water at a solid-liquid ratio of 1: (25-30) g / mL, and then placed in a constant temperature water bath, heated and stirred at 80-85°C for 2-3 hours, and then filtered to obtain filtrate I and filter residue. The filter residue and distilled water are mixed evenly at a solid-liquid ratio of 1: (15-20) g / mL, heated and stirred in a constant temperature water bath at 80-85°C for a second time for 1-1.5 hours, and then filtered to obtain filtrate II. After mixing filtrate I and filtrate II, the mixture is filtered using gauze with a pore size of 0.2-0.5 mm, and then concentrated in a rotary evaporator at 70-75°C to obtain Tremella fuciformis extract; S2.2: The stored Lactobacillus kumiss subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution were placed at 30-35°C for 1-2 hours, and then the Lactobacillus kumiss subsp. ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution were transferred to MRS medium, PDA medium and beef extract peptone medium for activation culture for 12-15 hours under sterile conditions. The volume ratio of the bacterial solution to the culture medium is 1: (450-500), and then the obtained activated bacterial solution is inoculated into the corresponding culture medium at an inoculum amount of 4-5% for 24-30 hours of expansion culture, and the expanded cultured Lactobacillus kumiss subsp. kumiss ZW3 bacterial solution, Kluyveromyces marxianus bacterial solution and Bacillus subtilis DE111 bacterial solution are mixed and shaken evenly at a volume ratio of 1: (0.6-0.8): (0.3-0.4) to obtain a composite bacterial solution; S2.3: Place the Tremella extract in a fermentation container, add 4-5wt% fructose and 6-6.5wt% ammonium citrate, and then sterilize at 121-125℃ for 25-30 minutes. After cooling, inoculate the composite bacterial liquid prepared in step S2.2 at an inoculum amount of 10-12%, place it at a temperature of 30-35℃ and a rotation speed of 120-150rpm for 40-48 hours, and then centrifuge it at a speed of 8000-10000rpm, filter it to obtain the supernatant, place it in a water bath at 65-70℃ and heat it for 30-35 minutes to obtain the flavor and moisturizing Tremella polysaccharide fermentation liquid, and store it at 3-4℃.

4. The method for preparing a composition capable of improving the water-retaining and water-locking ability of the skin according to claim 3, characterized in that: Step S3, the light enzymatic hydrolysis of salmon nasal cartilage and the compounding of Tremella polysaccharide fermentation liquid, comprises the following steps: S3.1: Place the starch salmon nasal cartilage composite powder and distilled water in a container at a material-liquid ratio of 1: (8-10) g / mL, adjust the pH to 8.5-9, heat to 48-50°C, add trypsin and flavor protease, wherein the enzyme dosage of trypsin is 3500-4000 U / g, and the enzyme dosage of flavor protease is 2500-3000 U / g, and after enzymolysis for 1.5-2 hours, heat at 95-100°C for 10-15 minutes to obtain a light enzymatic hydrolyzate of salmon nasal cartilage powder; S3.2: Take flavored moisturizing Tremella polysaccharide fermentation liquid, and add 85-95% of salmon nasal cartilage powder light enzymatic hydrolyzate, 10-15% of maltodextrin, 2-5% of Cherry blossom extract, 1-5% of low-methoxyl pectin, 0.1-0.3% of niacinamide and 1.5-2% of erythritol, based on the mass percentage of the flavored moisturizing Tremella polysaccharide fermentation liquid, mix well, and then vacuum freeze-dry at -60°C to -40°C to obtain a composition that can improve the skin's ability to retain water.

5. The method for preparing a composition capable of improving the water-retaining and water-locking ability of the skin according to claim 2, characterized in that: Step S1.1: Cut the salmon nasal cartilage into small pieces with a particle size of 3-5 cm.

6. The method for preparing a composition capable of improving the water-retaining and water-locking ability of the skin according to claim 2, characterized in that: Step S1.2: When adding the deodorized salmon nasal cartilage to the disodium ethylenediaminetetraacetic acid solution and the isopropanol solution, the temperature is maintained at 3-4°C.

7. The method for preparing a composition capable of improving the water-retaining and water-locking ability of the skin according to claim 3, characterized in that: Step S2.1: Mix filtrate I and filtrate II and then perform rotary evaporation to concentrate the volume of the filtrate I and filtrate II to 1 / 8-1 / 7 of the original volume.

8. The method for preparing a composition capable of improving the water-retaining and water-locking ability of the skin according to claim 3, characterized in that: In step S2.2, the activation and expansion culture temperature of Lactobacillus kruskalensis subsp. kruskalensis ZW3 bacterial solution and Kluyveromyces marxianus bacterial solution were both 30°C, and the activation and expansion culture temperature of Bacillus subtilis DE111 bacterial solution was 37°C.

9. The method for preparing a composition capable of improving the water-retaining and water-locking ability of the skin according to claim 4, characterized in that: The pH in step S3.1 is adjusted by adding a sodium hydroxide solution with a concentration of 0.1-0.15 mol / L.

10. A composition capable of improving the water-retaining and water-locking ability of the skin, characterized in that: The composition is prepared by the method for preparing a composition capable of improving the water retention and water locking ability of the skin as described in any one of claims 1 to 9.

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