Composition containing probiotics and application of composition in preparation of weight-losing products

Abstract

The invention belongs to the technical field of health care products, and particularly relates to a composition containing probiotics. Comprising the following components in parts by weight: 5-10 parts of mixed zymolyte, 2-3 parts of lactobacillus acidophilus, 1-2 parts of bifidobacterium lactis, 1-2 parts of lactobacillus bulgaricus, 1-2 parts of lactobacillus brevis, 1-3 parts of lactobacillus delbrueckii, 1-2 parts of mannitol, 1-2 parts of isomaltooligosacharide and 3-7 parts of mixed leavening. The composition prepared by the invention has a very good weight-losing effect, and especially has a remarkable effect on a large cardinal number crowd with the weight of more than 80kg.

Description

Technical Field

[0001] The invention belongs to the technical field of health care products, and particularly relates to a composition containing probiotics and application of the composition in preparing a weight loss product. Background Art

[0002] The need to lose weight is mainly due to comprehensive considerations of health, appearance and psychology. First, from a health perspective, being overweight or obese is a risk factor for a variety of chronic diseases, including cardiovascular disease, type 2 diabetes, metabolic syndrome, certain cancers, and bone and joint diseases. Obesity also increases the risk of breathing problems (such as sleep apnea syndrome) and puts extra pressure on joints, affecting quality of life.

[0003] The significance of weight loss supplements is to provide a convenient option for those who want to support the weight loss process through auxiliary means. Although a healthy diet and regular exercise are the basis of weight loss, some people may find it difficult to achieve the desired weight loss effect by lifestyle changes alone due to time constraints, metabolic problems or other factors. At this time, weight loss supplements can be used as a supplementary tool to help regulate metabolism, suppress appetite, promote fat burning or reduce fat absorption. For example, some supplements contain natural ingredients (such as green tea extract, L-carnitine, conjugated linoleic acid, etc.), which are believed to help accelerate fat metabolism or inhibit fat storage. In addition, weight loss supplements can also ensure that there is no lack of necessary nutrients due to dietary control during the weight loss process by supplementing vitamins and minerals. However, it should be noted that weight loss supplements are not a substitute for a healthy lifestyle. Their role is usually auxiliary and should be used under professional guidance to ensure safety and effectiveness. In general, the significance of weight loss supplements is to provide a scientific and reasonable auxiliary means for those who want additional support in the weight loss process to help them achieve their weight loss goals more easily and maintain a healthy weight in the long term. Although there are many types of weight loss products on the market, the effects of weight loss products on the market cannot meet the needs of consumers.

[0004] The following are the main reasons why a large number of people who want to lose weight have difficulty losing weight: 1. Reduced metabolic rate: Decreased basal metabolic rate: Long-term obesity can cause the body's basal metabolic rate (BMR) to decrease, which means that the body consumes less energy even when resting. As weight increases, the body gradually adapts to high calorie intake, causing metabolism to become slow. This metabolic adaptation makes it more difficult to lose weight because even if food intake is reduced, the body will maintain the existing weight by reducing energy consumption.

[0005] Therefore, there is an urgent need for a composition containing probiotics and its use in preparing weight loss products. Summary of the invention

[0006] The purpose of the present invention is to provide a composition containing probiotics and application of the composition in preparing a weight loss product.

[0007] In order to achieve the above object, the present invention provides the following technical solutions: A composition containing probiotics comprises the following components in parts by weight: 5-10 parts of mixed enzymolysate, 2-3 parts of Lactobacillus acidophilus, 1-2 parts of Bifidobacterium lactis, 1-2 parts of Lactobacillus bulgaricus, 1-2 parts of Lactobacillus breve, 1-3 parts of Lactobacillus delbrueckii, 1-2 parts of mannitol, 1-2 parts of isomaltooligosaccharide and 3-7 parts of mixed fermentation product.

[0008] Furthermore, the method for preparing the mixed enzymatic hydrolysate comprises the following steps: (1) Grinding white kidney beans, green tea and yam to a particle size of less than 100 mesh to obtain enzymatic hydrolysis raw materials; (2) Mixing the enzymatic hydrolysis raw material and water in a weight ratio of 1:(8-10) to obtain an enzymatic hydrolysis solution, adding cellulase to the enzymatic hydrolysis solution, and performing enzymatic hydrolysis for 40-50 minutes; adjusting the pH value to 9-10 using a 0.5-1 mol / L NaOH aqueous solution, adding alkaline protease, and performing enzymatic hydrolysis for 40-50 minutes; then adjusting the pH value to 8-9 using a 0.5-1 mol / L HCl aqueous solution, adding bromelain, and continuing enzymatic hydrolysis for 30-40 minutes; then adjusting the pH value to 5-6 using a 0.5-1 mol / L HCl aqueous solution, adding pectinase, and continuing enzymatic hydrolysis for 60-80 minutes, and obtaining an enzymatic hydrolysis product after inactivating the enzyme; (3) Centrifuging the enzymatic hydrolysate, taking the supernatant, filtering the supernatant through an ultrafiltration membrane to obtain an ultrafiltrate, concentrating and spray-drying to obtain a mixed enzymatic hydrolysate.

[0009] Further, white kidney bean, green tea and yam are mixed in a weight ratio of (0.3-0.5) :1:(1.4-1.7).

[0010] Furthermore, the addition amount of cellulase is 2000-3000u / mL enzymolysis solution; the addition amount of alkaline protease is 1000-2000u / mL enzymolysis solution; the addition amount of bromelain is 1000-2000u / mL enzymolysis solution; and the addition amount of pectinase is 1000-2000u / mL enzymolysis solution.

[0011] Further, the supernatant is filtered through an ultrafiltration membrane with a molecular weight of 4000-5000Da.

[0012] Furthermore, the method for preparing the mixed fermentation product comprises the following steps: (1) Grinding lotus leaves, hawthorns and citrus fruits to a particle size of less than 100 mesh to obtain a mixed raw material; (2) The mixed raw material is first placed in a low temperature of -30°C for 2-3 minutes, then rapidly heated to 23-25°C within 2 seconds, and repeated 2-3 times; then pressurized to 20-25MPa, maintained for 3-5 minutes, and then depressurized to normal pressure, and repeated 2-3 times to obtain a pretreated mixed raw material; (3) mixing the pretreated mixed raw material and water in a weight ratio of 1:(8-10) to obtain a mixed solution, adding composite bacteria to the mixed solution, performing anaerobically fermentation for 10-15 hours, and sterilizing to obtain a fermentation liquid; (4) Centrifuging the fermentation broth, taking the supernatant, filtering the supernatant through an ultrafiltration membrane with a molecular weight of 3000-5000Da to obtain an ultrafiltrate, concentrating and spray-drying to obtain a mixed fermentation product.

[0013] Further, lotus leaves, hawthorns and citrus are mixed in a weight ratio of 1:(1.2-1.5):(0.4-0.7).

[0014] Furthermore, the composite bacteria include Lactobacillus casei, Lactobacillus brevis and Bifidobacterium longum; the addition amount of Lactobacillus casei is 10 7 -10 8 CFU / mL mixed solution; the amount of Lactobacillus brevis added was 10 9 -10 10 CFU / mL mixed solution; the amount of Bifidobacterium longum added was 10 8 -10 9 CFU / mL mixed solution.

[0015] Furthermore, the preparation method of the probiotics-containing composition comprises the following steps: weighing the components according to weight ratio, and mixing them evenly to obtain the composition.

[0016] The invention provides application of the probiotic-containing composition in preparing a weight-loss product.

[0017] Compared with the prior art, the advantages and beneficial effects of the present invention are: 1. In the mixed hydrolysate prepared by enzymatic hydrolysis of the present invention, white kidney bean contains rich α-amylase inhibitors, which can inhibit the activity of α-amylase in the small intestine and delay the digestion and absorption of carbohydrates. This helps to prevent the rapid increase of blood sugar, reduce the secretion of insulin, and avoid further aggravation of insulin resistance. By inhibiting α-amylase, white kidney bean extract can reduce the sharp rise of blood sugar after a meal, stabilize blood sugar levels, and reduce hunger and overeating. Catechins (such as EGCG) in green tea have a strong antioxidant effect, which can activate lipolytic enzymes in fat cells and promote fat oxidation and metabolism. Studies have shown that green tea extract can increase energy consumption and reduce fat accumulation, especially in the abdominal area. Polyphenols in green tea can also improve insulin sensitivity, help the body better utilize glucose, and reduce fat storage. In addition, green tea also has anti-inflammatory effects, which can reduce chronic inflammation associated with obesity and further improve metabolic health. Polysaccharides in yam can improve insulin sensitivity, help the body better utilize glucose, and reduce fat storage. In addition, yams have anti-inflammatory effects, which can reduce chronic inflammation associated with obesity and further improve the symptoms of metabolic syndrome.

[0018] 2. In the composition of the present invention, lotus leaf, hawthorn and citrus are mixed and fermented with specific bacteria. The obtained product can help a large number of people weighing more than 80 kg to lose weight through various means. Lotus leaf is rich in alkaloids (such as nuciferine), which can inhibit the intestinal absorption of fat and reduce the accumulation of fat in the body. During the fermentation process, specific bacteria can further decompose the complex components in the lotus leaf, release more active substances, and enhance its effect of inhibiting fat absorption. The flavonoids in lotus leaves have antioxidant effects, can activate lipolytic enzymes in fat cells, and promote fat oxidation and metabolism. The fermentation process can increase the bioavailability of these flavonoids, making them more easily absorbed by the human body, thereby accelerating fat burning.

[0019] Hawthorn is rich in organic acids (such as chlorogenic acid and caffeic acid) and lipase, which can promote gastric juice secretion and speed up the digestion of food, especially the digestion of greasy food. During the fermentation process, specific bacteria can further decompose the organic acids in hawthorn, produce more metabolites, and enhance its effect of promoting digestion and fat metabolism. The flavonoids and triterpenoids in hawthorn have the effect of lowering blood lipids, which can reduce the levels of triglycerides and low-density lipoprotein (LDL) cholesterol in the blood and reduce the risk of arteriosclerosis. The fermentation process can increase the biological activity of these compounds and enhance their effect of lowering blood lipids. Citrus is rich in vitamin C and flavonoids (such as hesperidin and naringin), which have strong antioxidant effects, can activate lipoxygenase in fat cells, and promote fat oxidation and metabolism. During the fermentation process, specific bacteria can further decompose the flavonoids in citrus, release more active substances, and enhance its effect of promoting fat oxidation.

[0020] Specific bacteria produce short-chain fatty acids (SCFAs) such as acetic acid, propionic acid, and butyric acid during the fermentation process. These short-chain fatty acids can not only serve as an energy source, but also regulate the balance of intestinal flora, promote the growth of beneficial bacteria, and inhibit the reproduction of harmful bacteria. Healthy intestinal flora helps improve metabolic health, reduce inflammatory responses, and promote the decomposition and metabolism of fat. The short-chain fatty acids produced during the fermentation process can also enhance the intestinal barrier function, reduce the absorption of harmful substances, and prevent the occurrence of leaky gut syndrome. This helps improve intestinal health, reduce chronic inflammation associated with obesity, and further promote fat burning.

[0021] 3. The present invention compounds Lactobacillus acidophilus, Bifidobacterium lactis, Lactobacillus bulgaricus, Lactobacillus brevis and Lactobacillus delbrueckii, which can significantly improve the weight loss effect. This composite flora promotes fat metabolism, improves intestinal health, regulates blood sugar and insulin sensitivity, and enhances satiety and reduces appetite from multiple aspects through multiple synergistic effects. Lactobacillus acidophilus can produce short-chain fatty acids (SCFAs), especially acetic acid and propionic acid, which can inhibit lipase in adipocytes and reduce the synthesis and storage of fat. In addition, Lactobacillus acidophilus can also promote the decomposition of fat by regulating intestinal flora, reducing endotoxins produced by harmful bacteria, and reducing chronic inflammation. Bifidobacterium lactis can also promote the decomposition and metabolism of fat by activating lipase in adipocytes, helping the body to consume fat more effectively. Lactobacillus bulgaricus can also maintain the balance of intestinal flora, reduce endotoxins produced by harmful bacteria, and reduce chronic inflammation by competitively inhibiting the growth of harmful bacteria. Lactobacillus brevis can enhance the intestinal barrier function, reduce the absorption of harmful substances and prevent the occurrence of intestinal leakage by producing mucin and tight junction proteins. This helps improve intestinal health, reduce chronic inflammation associated with obesity, and further promote fat burning. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Example 1

[0024] The present embodiment provides a composition containing probiotics, comprising the following components in parts by weight: 5 parts of mixed enzymatic hydrolysate, 2 parts of Lactobacillus acidophilus, 1 part of Bifidobacterium lactis, 1 part of Lactobacillus bulgaricus, 1 part of Lactobacillus breve, 1 part of Lactobacillus delbrueckii, 1 part of mannitol, 1 part of isomaltooligosaccharide, and 3 parts of mixed fermentation product.

[0025] The preparation method of the mixed enzymolyte comprises the following steps: (1) Grinding white kidney beans, green tea and yam to a particle size of less than 100 mesh to obtain enzymatic hydrolysis raw materials; (2) Mixing the enzymatic hydrolysis raw materials and water in a weight ratio of 1:8 to obtain an enzymatic hydrolysis solution, adding cellulase to the enzymatic hydrolysis solution, and performing enzymatic hydrolysis for 40 minutes; adjusting the pH value to 9 using a 0.5 mol / L NaOH aqueous solution, adding alkaline protease, and performing enzymatic hydrolysis for 40 minutes; then adjusting the pH value to 8 using a 0.5 mol / L HCl aqueous solution, adding bromelain, and continuing enzymatic hydrolysis for 30 minutes; then adjusting the pH value to 5 using a 0.5 mol / L HCl aqueous solution, adding pectinase, and continuing enzymatic hydrolysis for 60 minutes, and after inactivating the enzyme, obtaining an enzymatic hydrolysis product; (3) Centrifuging the enzymatic hydrolysate, taking the supernatant, filtering the supernatant through an ultrafiltration membrane to obtain an ultrafiltrate, concentrating and spray-drying to obtain a mixed enzymatic hydrolysate.

[0026] Mix white kidney beans, green tea and yam in a weight ratio of 0.3:1:1.4.

[0027] The addition amount of cellulase is 2000u / mL enzymatic solution; the addition amount of alkaline protease is 1000u / mL enzymatic solution; the addition amount of bromelain is 1000u / mL enzymatic solution; and the addition amount of pectinase is 1000u / mL enzymatic solution.

[0028] The supernatant was filtered through an ultrafiltration membrane with a molecular weight of 4000 Da.

[0029] The method for preparing the mixed fermentation product comprises the following steps: (1) Grinding lotus leaves, hawthorns and citrus fruits to a particle size of less than 100 mesh to obtain a mixed raw material; (2) The mixed raw material is first placed in a low temperature of -30°C for 2 minutes, then rapidly heated to 23°C within 2 seconds, and repeated twice; then pressurized to 20 MPa, maintained for 3 minutes, and then released to normal pressure, and repeated twice to obtain a pretreated mixed raw material; (3) mixing the pretreated mixed raw material and water in a weight ratio of 1:8 to obtain a mixed solution, adding composite bacteria to the mixed solution, performing anaerobic fermentation for 10 hours, and sterilizing to obtain a fermentation liquid; (4) Centrifuging the fermentation broth, taking the supernatant, filtering the supernatant through an ultrafiltration membrane with a molecular weight of 3000 Da to obtain an ultrafiltrate, concentrating and spray-drying to obtain a mixed fermentation product.

[0030] Mix lotus leaves, hawthorn and citrus in a weight ratio of 1:1.2:0.4.

[0031] The composite bacteria include Lactobacillus casei, Lactobacillus brevis and Bifidobacterium longum; the amount of Lactobacillus casei added is 10 7 CFU / mL mixed solution; the amount of Lactobacillus brevis added was 10 9 CFU / mL mixed solution; the amount of Bifidobacterium longum added was 10 8 CFU / mL mixed solution.

[0032] The preparation method of the composition containing probiotics comprises the following steps: weighing components according to weight ratio, and mixing them evenly to obtain the composition.

[0033] Example 2

[0034] The present embodiment provides a composition containing probiotics, comprising the following components in parts by weight: 10 parts of mixed enzymatic hydrolysate, 3 parts of Lactobacillus acidophilus, 2 parts of Bifidobacterium lactis, 2 parts of Lactobacillus bulgaricus, 2 parts of Lactobacillus breve, 3 parts of Lactobacillus delbrueckii, 2 parts of mannitol, 2 parts of isomaltooligosaccharide, and 7 parts of mixed fermentation product.

[0035] The preparation method of the mixed enzymolyte comprises the following steps: (1) Grinding white kidney beans, green tea and yam to a particle size of less than 100 mesh to obtain enzymatic hydrolysis raw materials; (2) Mixing the enzymatic hydrolysis raw materials and water in a weight ratio of 1:10 to obtain an enzymatic hydrolysis solution, adding cellulase to the enzymatic hydrolysis solution, and performing enzymatic hydrolysis for 50 minutes; adjusting the pH value to 10 using a 1 mol / L NaOH aqueous solution, adding alkaline protease, and performing enzymatic hydrolysis for 50 minutes; then adjusting the pH value to 9 using a 1 mol / L HCl aqueous solution, adding bromelain, and continuing enzymatic hydrolysis for 40 minutes; then adjusting the pH value to 6 using a 1 mol / L HCl aqueous solution, adding pectinase, and continuing enzymatic hydrolysis for 80 minutes, and after inactivating the enzyme, obtaining an enzymatic hydrolysis product; (3) Centrifuging the enzymatic hydrolysate, taking the supernatant, filtering the supernatant through an ultrafiltration membrane to obtain an ultrafiltrate, concentrating and spray-drying to obtain a mixed enzymatic hydrolysate.

[0036] Mix white kidney beans, green tea and yam in a weight ratio of 0.5:1:1.7.

[0037] The addition amount of cellulase is 3000u / mL enzymatic solution; the addition amount of alkaline protease is 2000u / mL enzymatic solution; the addition amount of bromelain is 2000u / mL enzymatic solution; and the addition amount of pectinase is 2000u / mL enzymatic solution.

[0038] The supernatant was filtered through an ultrafiltration membrane with a molecular weight of 5000 Da.

[0039] The method for preparing the mixed fermentation product comprises the following steps: (1) Grinding lotus leaves, hawthorns and citrus fruits to a particle size of less than 100 mesh to obtain a mixed raw material; (2) The mixed raw material is first placed in a low temperature of -30°C for 3 minutes, then rapidly heated to 25°C within 2 seconds, and repeated 3 times; then pressurized to 25 MPa, maintained for 5 minutes, and then released to normal pressure, and repeated 3 times to obtain a pretreated mixed raw material; (3) mixing the pretreated mixed raw material and water in a weight ratio of 1:10 to obtain a mixed solution, adding composite bacteria to the mixed solution, performing anaerobically fermentation for 15 hours, and sterilizing to obtain a fermentation liquid; (4) Centrifuging the fermentation broth, taking the supernatant, filtering the supernatant through an ultrafiltration membrane with a molecular weight of 5000 Da to obtain an ultrafiltrate, concentrating and spray-drying to obtain a mixed fermentation product.

[0040] Mix lotus leaves, hawthorn and citrus in a weight ratio of 1:1.5:0.7.

[0041] The composite bacteria include Lactobacillus casei, Lactobacillus brevis and Bifidobacterium longum; the amount of Lactobacillus casei added is 10 8 CFU / mL mixed solution; the amount of Lactobacillus brevis added was 10 10 CFU / mL mixed solution; the amount of Bifidobacterium longum added was 10 9 CFU / mL mixed solution.

[0042] The preparation method of the composition containing probiotics comprises the following steps: weighing components according to weight ratio, and mixing them evenly to obtain the composition.

[0043] Comparative Example 1 The difference between this comparative example and Example 1 is: a probiotic-containing composition, comprising the following components in parts by weight: 3 parts of mixed enzymatic hydrolysate, 4 parts of Lactobacillus acidophilus, 3 parts of Bifidobacterium lactis, 3 parts of Lactobacillus delbrueckii, 1 part of mannitol, 1 part of isomaltooligosaccharide, and 1 part of mixed fermentation product.

[0044] Comparative Example 2 The difference between this comparative example and Example 1 is that white kidney bean, green tea and yam are mixed in a weight ratio of 1:1:1.

[0045] Comparative Example 3 The difference between this comparative example and Example 1 is: the addition amount of cellulase is 1000u / mL enzymolysis solution; the addition amount of alkaline protease is 3000u / mL enzymolysis solution; the addition amount of bromelain is 500u / mL enzymolysis solution; and the addition amount of pectinase is 500u / mL enzymolysis solution.

[0046] Comparative Example 4 The difference between this comparative example and Example 1 is that lotus leaves, hawthorns and citrus are mixed in a weight ratio of 1:1:1.

[0047] Comparative Example 5 The difference between this comparative example and Example 1 is that the composite bacteria include Lactobacillus casei, Lactobacillus brevis and Bifidobacterium longum; the addition amount of Lactobacillus casei is 10 9 CFU / mL mixed solution; the amount of Lactobacillus brevis added was 10 8 CFU / mL mixed solution; the amount of Bifidobacterium longum added was 10 7 CFU / mL mixed solution.

[0048] Performance Testing 1. Experimental subjects and methods 1.1 Experimental subjects All subjects in this experiment were evaluated for body mass index before the experiment and were all obese. Among the 80 subjects, there were 40 males and 40 females; their ages ranged from 18 to 50, with an average age of (34.7±7.8) years old.

[0049] 1.2 Experimental methods Grouping: 80 subjects were randomly divided into 8 groups, negative control group (N=10), Example 1 (N=10), Example 2 (N=10), Comparative Example 1 (N=10), Comparative Example 2 (N=10), Comparative Example 3 (N=10), Comparative Example 4 (N=10), Comparative Example 5 (N=10). The negative control group took a placebo, 2 capsules / day; the examples and comparative examples were taken for 8 consecutive weeks according to the above method. During the entire experimental period, the diet was not controlled, exercise was not deliberately increased, and other weight loss foods and drugs were prohibited.

[0050] 1.3 Test indicators 1.3.1 Body composition test: Before and after the experiment, the subjects' weight, body fat percentage, and body mass index were tested using a DX200 super-strong body composition tester every week.

[0051] 1.4 Statistics The obtained data were processed by Excel, and the test data were statistically analyzed by single-blind method and t-test, and expressed as mean±SD.

[0052] The results are shown in Table 1.

[0053] Table 1: Changes in weight of subjects before and after the experiment

[0054] It can be seen from Table 1 that the composition of the present invention has an excellent weight loss effect.

[0055] By comparing Example 1 with Comparative Example 1, it can be seen that the ratio and composition of the composition of the present invention can significantly improve the weight loss effect.

[0056] By comparing Example 1 with Comparative Examples 2-3, it can be seen that the mixed enzymolysate prepared by the specific enzymolysis method of the present invention significantly improves the weight loss effect.

[0057] By comparing Example 1 with Comparative Examples 4-5, it can be seen that the mixed fermented product prepared by the specific fermentation method of the present invention significantly improves the weight loss effect.

[0058] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition containing probiotics, characterized in that: The invention comprises the following components in parts by weight: 5-10 parts of mixed enzymatic hydrolysate, 2-3 parts of lactobacillus acidophilus, 1-2 parts of bifidobacterium lactis, 1-2 parts of lactobacillus bulgaricus, 1-2 parts of lactobacillus breve, 1-3 parts of lactobacillus delbrueckii, 1-2 parts of mannitol, 1-2 parts of isomaltooligosaccharide and 3-7 parts of mixed fermentation product.

2. The composition containing probiotics according to claim 1, characterized in that The preparation method of the mixed enzymolyte comprises the following steps: (1) Grinding white kidney beans, green tea and yam to a particle size of less than 100 mesh to obtain enzymatic hydrolysis raw materials; (2) Mixing the enzymatic hydrolysis raw material and water in a weight ratio of 1:(8-10) to obtain an enzymatic hydrolysis solution, adding cellulase to the enzymatic hydrolysis solution, and performing enzymatic hydrolysis for 40-50 minutes; adjusting the pH value to 9-10 using a 0.5-1 mol / L NaOH aqueous solution, adding alkaline protease, and performing enzymatic hydrolysis for 40-50 minutes; then adjusting the pH value to 8-9 using a 0.5-1 mol / L HCl aqueous solution, adding bromelain, and continuing enzymatic hydrolysis for 30-40 minutes; then adjusting the pH value to 5-6 using a 0.5-1 mol / L HCl aqueous solution, adding pectinase, and continuing enzymatic hydrolysis for 60-80 minutes, and obtaining an enzymatic hydrolysis product after inactivating the enzyme; (3) Centrifuging the enzymatic hydrolysate, taking the supernatant, filtering the supernatant through an ultrafiltration membrane to obtain an ultrafiltrate, concentrating and spray-drying to obtain a mixed enzymatic hydrolysate.

3. The composition containing probiotics according to claim 2, characterized in that Mix white kidney beans, green tea and yam in a weight ratio of (0.3-0.5) :1:(1.4-1.7).

4. The composition containing probiotics according to claim 2, characterized in that The addition amount of cellulase is 2000-3000u / mL enzymolysis solution; the addition amount of alkaline protease is 1000-2000u / mL enzymolysis solution; the addition amount of bromelain is 1000-2000u / mL enzymolysis solution; and the addition amount of pectinase is 1000-2000u / mL enzymolysis solution.

5. The composition containing probiotics according to claim 2, characterized in that The supernatant was filtered through an ultrafiltration membrane with a molecular weight of 4000-5000Da.

6. The composition containing probiotics according to claim 1, characterized in that The method for preparing the mixed fermentation product comprises the following steps: (1) Grinding lotus leaves, hawthorns and citrus fruits to a particle size of less than 100 mesh to obtain a mixed raw material; (2) The mixed raw material is first placed in a low temperature of -30°C for 2-3 minutes, then rapidly heated to 23-25°C within 2 seconds, and repeated 2-3 times; then pressurized to 20-25MPa, maintained for 3-5 minutes, and then depressurized to normal pressure, and repeated 2-3 times to obtain a pretreated mixed raw material; (3) mixing the pretreated mixed raw material and water in a weight ratio of 1:(8-10) to obtain a mixed solution, adding composite bacteria to the mixed solution, performing anaerobically fermentation for 10-15 hours, and sterilizing to obtain a fermentation liquid; (4) Centrifuging the fermentation broth, taking the supernatant, filtering the supernatant through an ultrafiltration membrane with a molecular weight of 3000-5000Da to obtain an ultrafiltrate, concentrating and spray-drying to obtain a mixed fermentation product.

7. The composition containing probiotics according to claim 6, characterized in that Mix lotus leaves, hawthorn and citrus in a weight ratio of 1:(1.2-1.5):(0.4-0.7).

8. The composition containing probiotics according to claim 6, characterized in that The composite bacteria include Lactobacillus casei, Lactobacillus brevis and Bifidobacterium longum; the amount of Lactobacillus casei added is 10 7 -10 8 CFU / mL mixed solution; the amount of Lactobacillus brevis added was 10 9 -10 10 CFU / mL mixed solution; the amount of Bifidobacterium longum added was 10 8 -10 9 CFU / mL mixed solution.

9. The composition containing probiotics according to claim 1, characterized in that The preparation method of the composition containing probiotics comprises the following steps: weighing components according to weight ratio, and mixing them evenly to obtain the composition.

10. Use of the probiotic composition according to any one of claims 1 to 9 in the preparation of a weight loss product.

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