Sterile liquid dressing and preparation method thereof

Abstract

The invention relates to the technical field of medical instruments, and discloses a sterile liquid dressing and a preparation method thereof.EGF, silicone rubber modified chitosan, epoxy group modified quaternized boric acid, polyacrylic resin IV, ethyl acetate, menthol, polyethylene glycol and purified water are added into a stirrer and stirred, and the sterile liquid dressing is obtained. In the stirring process, a large number of epoxy groups in the epoxy group modified quaternized boric acid and phenolic hydroxyl groups in the silicone rubber modified chitosan are subjected to a ring-opening reaction, cross-linking is generated between the epoxy groups and the phenolic hydroxyl groups, the epoxy groups and the phenolic hydroxyl groups can be better adsorbed on the surface of the skin, the film forming effect is better, and the repairing and antibacterial effects on the skin are promoted. Chitosan in the silicone rubber modified chitosan and quaternary ammonium salt groups in the epoxy group modified quaternized boric acid have good antibacterial effects, and the substitution degree of quaternary ammonium salt in the epoxy group modified quaternized boric acid is high, so that the antibacterial effect is further improved.

Description

Technical Field

[0001] The invention relates to the technical field of medical devices, in particular to a sterile liquid dressing and a preparation method thereof. Background Art

[0002] Medical dressings are a type of medical material used to temporarily replace skin after skin damage and protect wounds. They can prevent wound infection and dehydration, provide a moist environment that is conducive to wound healing, and promote tissue repair. Silicone rubber has good biocompatibility and does not produce adverse reactions or irritation to human tissue. At the same time, silicone rubber can help dry human skin. Therefore, it is used in the sterile liquid dressing of the present invention to accelerate the recovery of wounds.

[0003] For example, patent CN112107724 discloses a liquid dressing and a preparation method thereof. The liquid dressing prepared by the invention is safe and non-irritating, and can protect the wound from external pollutants and bacterial infection, while also playing a role in moisturizing and repairing the wound skin. However, its antibacterial effect needs to be improved. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies of the prior art, the present invention provides a sterile liquid dressing and a preparation method thereof, which has good film-forming, antibacterial and repairing effects.

[0006] (II) Technical solution

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: a sterile liquid dressing, comprising the following components by weight: 0.001-0.002 parts by weight of EGF, 2-3 parts by weight of silicone rubber modified chitosan, 1-3 parts by weight of epoxy modified quaternary ammonium boric acid, 8-11 parts by weight of polyacrylic acid resin IV, 3-6 parts by weight of ethyl acetate, 0.4-0.6 parts by weight of menthol, 5-9 parts by weight of polyethylene glycol, and 40-60 parts by weight of purified water.

[0008] Preferably, the preparation method of the silicone rubber modified chitosan is:

[0009] Step 1: dissolving magnolol and 3-mercaptopropionic acid in 2-4 ml of dichloromethane under nitrogen protection, stirring evenly, and then adding benzoin dimethyl ether catalyst thereto, irradiating with 352-365 nm ultraviolet light for 2-4 h in a dark environment at a power of 40 W, and then purifying the product with a chromatographic column to obtain carboxylated magnolol;

[0010] Step 2: dissolving carboxylated magnolol and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt in 5-7 ml of ethanol solvent, stirring at room temperature for 25-40 min, then adding N-hydroxysuccinimide, reacting in an ice-water bath for 1-2 h, continuing to add 10-13 ml of chitosan hydrochloride mixed solution, controlling the pH at 4-5.8 at room temperature, reacting for 20-24 h, centrifuging the product, and washing it with ethanol, and finally dialyzing and freeze-drying to obtain magnolol-modified chitosan;

[0011] Step 3: Add 20-30 ml of methanol solvent, 0.12-0.14 mmol of inhibitor butylhydroxytoluene, and 0.23-0.27 mmol of catalyst chloroplatinic acid in tetrahydrofuran solution into the reactor, stir for 2-4 min, continue to add 2.1-2.6 mmol of magnolol-modified chitosan and 1.9-2.2 mmol of silicone rubber, ultrasonically disperse for 30-40 min, react at 65-80 ° C for 4-6 h, cool, centrifuge, and wash to obtain silicone rubber-modified chitosan.

[0012] In the above reaction process, the terminal alkenyl group in magnolol and the thiol group of 3-mercaptopropionic acid undergo a click reaction to introduce a carboxyl group and an alkenyl group to obtain carboxylated magnolol, which is then subjected to an amidation reaction with a chitosan hydrochloride mixture, and finally subjected to an addition reaction with silicone rubber to obtain silicone rubber-modified chitosan.

[0013] Preferably, in step 1, the molar ratio of magnolol, 3-mercaptopropionic acid, and benzoin dimethyl ether catalyst is (1.3-1.4) mmol:1 mmol:(0.2-0.3) mmol.

[0014] Preferably, in the step 2, the molar ratio of carboxylated magnolol, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt, and N-hydroxysuccinimide is 1 mmol:(0.1-0.2) mmol:(0.1-0.3) mmol.

[0015] Preferably, the preparation method of the epoxy-modified quaternary ammonium boric acid is:

[0016] S1. Add boric acid and 1,3-di(dimethylamino)-2-propanol to 25-35 ml of acetone solvent, stir and disperse, react at 95-110° C. for 6-8 hours, and after the reaction, distill under reduced pressure, filter and dry to obtain tertiary amine boronic acid;

[0017] S2. Add tertiary amine boronic acid and 3-chloropropylene to 20-40 ml of N,N-dimethylformamide solvent, stir and disperse, react at 70-85° C. for 10-12 h, and then distill under reduced pressure and filter to obtain alkenyl quaternary ammonium boronic acid;

[0018] S3. At 25-30°C, add 8-12 mmol of olefin quaternary ammonium boric acid to 20-35 ml of N,N-dimethylformamide solvent, stir evenly, then add 40-55 mmol of aqueous hydrogen peroxide solution, stir and react for 3-5 hours. After the reaction is completed, let stand, distill under reduced pressure, and wash to obtain epoxy-modified quaternary ammonium boric acid.

[0019] In the above reaction process, boric acid and the hydroxyl group in 1,3-di(dimethylamino)-2-propanol are subjected to esterification reaction to introduce a tertiary amine group to obtain tertiary aminoboric acid, which is then further subjected to quaternization reaction with the chlorine in 3-chloropropylene to generate a quaternary ammonium salt, and an olefin group is further introduced. Finally, an epoxidation reaction is carried out under the action of aqueous hydrogen peroxide to obtain epoxy-modified quaternary ammonium boric acid.

[0020] Preferably, the molar ratio of boric acid to 1,3-bis(dimethylamino)-2-propanol in S1 is 1 mmol:(2.14-2.86) mmol.

[0021] Preferably, the molar ratio of tertiary amine boronic acid to 3-chloropropylene in S2 is 1 mmol:(3.5-4.2) mmol.

[0022] Preferably, the preparation method of the sterile liquid dressing is: add EGF, silicone rubber modified chitosan, epoxy modified quaternary ammonium boric acid, polyacrylic resin IV, ethyl acetate, menthol, polyethylene glycol, and purified water into a stirrer, and stir at 20-30° C. for 3-5 minutes to obtain a sterile liquid dressing.

[0023] (III) Beneficial technical effects

[0024] The invention prepares a sterile liquid dressing by adding EGF, silicone rubber modified chitosan, epoxy modified quaternary ammonium boric acid, polyacrylic resin IV, ethyl acetate, menthol, polyethylene glycol and purified water into a stirrer and stirring.

[0025] During the stirring process, a large number of epoxy groups in epoxy-modified quaternary ammonium boric acid will undergo a ring-opening reaction with the phenolic hydroxyl groups in silicone rubber-modified chitosan, resulting in cross-linking between each other, better adsorption on the surface of the skin, making it more film-forming and promoting skin repair and antibacterial effects. Both the chitosan in silicone rubber-modified chitosan and the quaternary ammonium salt groups in epoxy-modified quaternary ammonium boric acid have good antibacterial effects, and the quaternary ammonium salt substitution degree in epoxy-modified quaternary ammonium boric acid is high, which further improves its antibacterial effect. Silicone rubber has good biocompatibility and good adsorption capacity, and can be used as a protective layer to reduce direct contact of external bacteria with the skin. The boron element and magnolol structure in the sterile liquid dressing also have anti-inflammatory and antibacterial effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is the reaction formula for epoxy-modified quaternary ammonium boric acid. DETAILED DESCRIPTION

[0027] Preparation of chitosan hydrochloride mixed solution: 0.3 g of chitosan hydrochloride was added to 15 ml of ethanol / water (2 / 1, v / v) mixed solution to form a chitosan hydrochloride mixed solution.

[0028] Example 1

[0029] (1) 6.5 mmol of magnolol and 5 mmol of 3-mercaptopropionic acid were dissolved in 2 ml of dichloromethane under nitrogen protection, and then 1 mmol of benzoin dimethyl ether catalyst was added thereto. In a dark environment, the mixture was irradiated with 352 nm ultraviolet light for 2 h at a power of 40 W. After the irradiation, the product was purified by a chromatographic column to obtain carboxylated magnolol;

[0030] (2) 3 mmol of carboxylated magnolol and 0.3 mmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt were dissolved in 5 ml of ethanol solvent, stirred at room temperature for 25 min, then 0.3 mmol of N-hydroxysuccinimide was added, and the mixture was reacted in an ice-water bath for 1 h, and 10 ml of chitosan hydrochloride mixture was added, the pH was controlled to 4 at room temperature, and the mixture was reacted for 20 h. The product was centrifuged and washed with ethanol, and finally dialyzed and freeze-dried to obtain magnolol-modified chitosan;

[0031] (3) Add 20 ml of methanol solvent, 0.12 mmol of inhibitor dibutyl hydroxytoluene, and 0.23 mmol of catalyst chloroplatinic acid in tetrahydrofuran solution to the reactor, stir for 2 min, continue to add 2.1 mmol of magnolol-modified chitosan and 1.9 mmol of silicone rubber, ultrasonically disperse for 30 min, react at 65 ° C for 4 h, cool, centrifuge, and wash to obtain silicone rubber-modified chitosan;

[0032] (4) adding 4 mmol of boric acid and 8.56 mmol of 1,3-di(dimethylamino)-2-propanol to 25 ml of acetone solvent, stirring and dispersing, reacting at 95° C. for 6 h, and after the reaction, distilling under reduced pressure, filtering and drying to obtain tertiary aminoboric acid;

[0033] (5) Add 2 mmol of tertiary amine boronic acid and 7 mmol of 3-chloropropylene to 20 ml of N,N-dimethylformamide solvent, stir and disperse, react at 70° C. for 10 h, and then perform vacuum distillation and filtration to obtain alkenyl quaternary ammonium boronic acid;

[0034] (6) At 25° C., 8 mmol of alkenyl quaternary ammonium boric acid was added to 20 ml of N,N-dimethylformamide solvent and stirred evenly. Then, a 40 mmol aqueous solution of hydrogen peroxide was added thereto and stirred for 3 h. After the reaction was completed, the mixture was allowed to stand, distilled under reduced pressure, and washed to obtain epoxy-modified quaternary ammonium boric acid;

[0035] (7) 0.001 g of EGF, 2 g of silicone rubber modified chitosan, 1 g of epoxy-modified quaternary ammonium boric acid, 8 g of polyacrylic acid resin IV, 3 g of ethyl acetate, 0.4 g of menthol, 5 g of polyethylene glycol, and 40 g of purified water were added into a blender and stirred at 20° C. for 3 min to obtain a sterile liquid dressing.

[0036] Example 2

[0037] (1) 7 mmol of magnolol and 5 mmol of 3-mercaptopropionic acid were dissolved in 4 ml of dichloromethane under nitrogen protection, and then 1.5 mmol of benzoin dimethyl ether catalyst was added thereto. In a dark environment, the mixture was irradiated with 365 nm ultraviolet light for 4 h at a power of 40 W. After the irradiation, the product was purified by a chromatographic column to obtain carboxylated magnolol;

[0038] (2) 3 mmol of carboxylated magnolol and 0.6 mmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt were dissolved in 7 ml of ethanol solvent, stirred at room temperature for 40 min, then 0.9 mmol of N-hydroxysuccinimide was added, and the mixture was reacted in an ice-water bath for 2 h, and 13 ml of chitosan hydrochloride mixture was added, the pH was controlled to 5.8 at room temperature, and the mixture was reacted for 24 h. The product was centrifuged and washed with ethanol, and finally dialyzed and freeze-dried to obtain magnolol-modified chitosan;

[0039] (3) Add 30 ml of methanol solvent, 0.14 mmol of inhibitor dibutyl hydroxytoluene, and 0.27 mmol of catalyst chloroplatinic acid in tetrahydrofuran solution to the reactor, stir for 4 min, continue to add 2.6 mmol of magnolol-modified chitosan and 2.2 mmol of silicone rubber, ultrasonically disperse for 40 min, react at 80 ° C for 6 h, cool, centrifuge, and wash to obtain silicone rubber-modified chitosan;

[0040] (4) adding 4 mmol of boric acid and 11.44 mmol of 1,3-di(dimethylamino)-2-propanol to 35 ml of acetone solvent, stirring and dispersing, reacting at 110° C. for 8 h, and after the reaction, distilling under reduced pressure, filtering and drying to obtain tertiary aminoboric acid;

[0041] (5) Add 2 mmol of tertiary amine boronic acid and 8.4 mmol of 3-chloropropylene to 40 ml of N,N-dimethylformamide solvent, stir and disperse, react at 85° C. for 12 h, and then perform vacuum distillation and filtration to obtain alkenyl quaternary ammonium boronic acid;

[0042] (6) At 30° C., 12 mmol of alkenyl quaternary ammonium boric acid was added to 35 ml of N,N-dimethylformamide solvent, stirred evenly, and then 55 mmol of hydrogen peroxide aqueous solution was added thereto, stirred for reaction for 5 h, and after the reaction was completed, the mixture was allowed to stand, vacuum distilled, and washed to obtain epoxy-modified quaternary ammonium boric acid;

[0043] (7) 0.002 g of EGF, 3 g of silicone rubber modified chitosan, 3 g of epoxy-modified quaternary ammonium boric acid, 11 g of polyacrylic acid resin IV, 6 g of ethyl acetate, 0.6 g of menthol, 9 g of polyethylene glycol, and 60 g of purified water were added into a blender and stirred at 30° C. for 5 min to obtain a sterile liquid dressing.

[0044] Example 3

[0045] (1) 6.8 mmol of magnolol and 5 mmol of 3-mercaptopropionic acid were dissolved in 3 ml of dichloromethane under nitrogen protection, and then 1.2 mmol of benzoin dimethyl ether catalyst was added thereto. In a dark environment, the mixture was irradiated with 356 nm ultraviolet light for 3 h at a power of 40 W. After the irradiation, the product was purified by a chromatographic column to obtain carboxylated magnolol;

[0046] (2) 3 mmol of carboxylated magnolol and 0.4 mmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt were dissolved in 6 ml of ethanol solvent, stirred at room temperature for 35 min, then 0.6 mmol of N-hydroxysuccinimide was added, and the mixture was reacted in an ice-water bath for 1 h, and 12 ml of chitosan hydrochloride mixture was added, the pH was controlled to 5.8 at room temperature, and the mixture was reacted for 22 h. The product was centrifuged and washed with ethanol, and finally dialyzed and freeze-dried to obtain magnolol-modified chitosan;

[0047] (3) Add 25 ml of methanol solvent, 0.13 mmol of inhibitor dibutyl hydroxytoluene, and 0.25 mmol of catalyst chloroplatinic acid in tetrahydrofuran solution to the reactor, stir for 3 min, continue to add 2.3 mmol of magnolol-modified chitosan and 2.1 mmol of silicone rubber, ultrasonically disperse for 35 min, react at 70 ° C for 5 h, cool, centrifuge, and wash to obtain silicone rubber-modified chitosan;

[0048] (4) adding 4 mmol of boric acid and 9.56 mmol of 1,3-di(dimethylamino)-2-propanol to 30 ml of acetone solvent, stirring and dispersing, reacting at 100° C. for 7 h, and after the reaction, distilling under reduced pressure, filtering and drying to obtain tertiary aminoboric acid;

[0049] (5) Add 2 mmol of tertiary amine boronic acid and 8.2 mmol of 3-chloropropylene to 30 ml of N,N-dimethylformamide solvent, stir and disperse, react at 76° C. for 11 h, and then perform vacuum distillation and filtration to obtain alkenyl quaternary ammonium boronic acid;

[0050] (6) At 28° C., 10 mmol of alkenyl quaternary ammonium boric acid was added to 25 ml of N,N-dimethylformamide solvent, stirred evenly, and then 45 mmol of hydrogen peroxide aqueous solution was added thereto, stirred for 4 h, and after the reaction was completed, the mixture was allowed to stand, distilled under reduced pressure, and washed to obtain epoxy-modified quaternary ammonium boric acid;

[0051] (7) 0.001 g of EGF, 2.5 g of silicone rubber modified chitosan, 2 g of epoxy modified quaternary ammonium boric acid, 10 g of polyacrylic acid resin IV, 4 g of ethyl acetate, 0.5 g of menthol, 6 g of polyethylene glycol, and 50 g of purified water were added into a blender and stirred at 25° C. for 4 min to obtain a sterile liquid dressing.

[0052] Example 4

[0053] (1) 6.5 mmol of magnolol and 5 mmol of 3-mercaptopropionic acid were dissolved in 2 ml of dichloromethane under nitrogen protection, and then 1 mmol of benzoin dimethyl ether catalyst was added thereto. In a dark environment, the mixture was irradiated with 352 nm ultraviolet light for 2 h at a power of 40 W. After the irradiation, the product was purified by a chromatographic column to obtain carboxylated magnolol;

[0054] (2) 3 mmol of carboxylated magnolol and 0.3 mmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt were dissolved in 5 ml of ethanol solvent, stirred at room temperature for 25 min, then 0.3 mmol of N-hydroxysuccinimide was added, and the mixture was reacted in an ice-water bath for 1 h, and 10 ml of chitosan hydrochloride mixture was added, the pH was controlled to 4 at room temperature, and the mixture was reacted for 20 h. The product was centrifuged and washed with ethanol, and finally dialyzed and freeze-dried to obtain magnolol-modified chitosan;

[0055] (3) Add 30 ml of methanol solvent, 0.14 mmol of inhibitor dibutyl hydroxytoluene, and 0.27 mmol of catalyst chloroplatinic acid in tetrahydrofuran solution to the reactor, stir for 4 min, continue to add 2.6 mmol of magnolol-modified chitosan and 2.2 mmol of silicone rubber, ultrasonically disperse for 40 min, react at 80 ° C for 6 h, cool, centrifuge, and wash to obtain silicone rubber-modified chitosan;

[0056] (4) adding 4 mmol of boric acid and 11.44 mmol of 1,3-di(dimethylamino)-2-propanol to 35 ml of acetone solvent, stirring and dispersing, reacting at 110° C. for 8 h, and after the reaction, distilling under reduced pressure, filtering and drying to obtain tertiary aminoboric acid;

[0057] (5) Add 2 mmol of tertiary amine boronic acid and 8.2 mmol of 3-chloropropylene to 30 ml of N,N-dimethylformamide solvent, stir and disperse, react at 76° C. for 11 h, and then perform vacuum distillation and filtration to obtain alkenyl quaternary ammonium boronic acid;

[0058] (6) At 28° C., 10 mmol of alkenyl quaternary ammonium boric acid was added to 25 ml of N,N-dimethylformamide solvent, stirred evenly, and then 45 mmol of hydrogen peroxide aqueous solution was added thereto, stirred for 4 h, and after the reaction was completed, the mixture was allowed to stand, distilled under reduced pressure, and washed to obtain epoxy-modified quaternary ammonium boric acid;

[0059] (7) 0.002 g of EGF, 2.5 g of silicone rubber modified chitosan, 2 g of epoxy-modified quaternary ammonium boric acid, 10 g of polyacrylic acid resin IV, 4 g of ethyl acetate, 0.5 g of menthol, 6 g of polyethylene glycol, and 50 g of purified water were added into a blender and stirred at 25° C. for 4 min to obtain a sterile liquid dressing.

[0060] Comparative Example 1

[0061] The difference between this comparative example and Example 4 is that no silicone rubber-modified chitosan is added.

[0062] Comparative Example 2

[0063] The difference between this comparative example and Example 4 is that no epoxy-modified quaternary ammonium boric acid is added.

[0064] The dressing of the sterile liquid dressing was dissolved and cast into a film to make a thin sheet with a radius of 1 cm; then the bacteria were transferred to a sterile culture medium using an inoculation loop to culture, making a bacterial suspension with a concentration of 106 CFU / mL, and then 0.5 mL of the bacterial suspension was transferred dropwise to a culture dish containing beef extract peptone culture medium, and after shaking evenly, the thin sheet was added and cultured in a constant temperature incubator. The diameter of the inhibition zone was measured after 48 hours of culture.

[0065] Table 1: Antimicrobial testing.

[0066] project Escherichia coli (mm) Staphylococcus aureus (mm) Example 1 15.2 15.8 Example 2 17.3 17.4 Example 3 15.4 16.7 Example 4 15.6 15.2 Comparative Example 1 12.1 11.3 Comparative Example 2 12.3 13.5

[0067] It can be seen from Table 1 that the sterile liquid dressing embodiments 1-4 of the present invention have better antibacterial effects than comparative examples 1-2.

[0068] At 15-20°C, 0.2 ml of the liquid dressings of Examples 1-4 and Comparative Examples 1-2 were sprayed evenly onto a plate (90 mm) to an area of ​​about 2 cm. 2 , the time for drying and solidification into film, and repeated the experiment twice. The average of the three experiments was taken. The results are shown in Table 2.

[0069] Table 2: Film formation time.

[0070] project Film forming time (min) Example 1 3min Example 2 2min15s Example 3 2min48s Example 4 3min14s Comparative Example 1 4min25s Comparative Example 2 4min34s

[0071] It can be seen from Table 2 that the sterile liquid dressing embodiments 1-4 of the present invention have better film-forming effects than comparative examples 1-2.

[0072] The liquid dressings of Examples 1-4 and Comparative Examples 1-2 were used as the experimental group and were applied to the wound skin (2 cm) of 10 SD rats (half male and half female, weighing 148.2 ± 12.5 g). 2 ) surface, 1 mL per mouse. The curing film formation time and repair time were observed. The results are shown in Table 3.

[0073] Table 3: Repair time.

[0074] project Repair time (d) Example 1 2-4 Example 2 1-2 Example 3 2-3 Example 4 2-3 Comparative Example 1 4-6 Comparative Example 2 4-5

[0075] It can be seen from Table 3 that the sterile liquid dressing embodiments 1-4 of the present invention have better repair effects than comparative examples 1-2.

[0076] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A sterile liquid dressing, characterized in that: The invention comprises the following components by weight: 0.001-0.002 parts by weight of EGF, 2-3 parts by weight of silicone rubber modified chitosan, 1-3 parts by weight of epoxy modified quaternary ammonium boric acid, 8-11 parts by weight of polyacrylic resin IV, 3-6 parts by weight of ethyl acetate, 0.4-0.6 parts by weight of menthol, 5-9 parts by weight of polyethylene glycol and 40-60 parts by weight of purified water.

2. The sterile liquid dressing according to claim 1, characterized in that: The preparation method of the silicone rubber modified chitosan is: Step 1: dissolving magnolol and 3-mercaptopropionic acid in dichloromethane under nitrogen protection, stirring evenly, and then adding benzoin dimethyl ether catalyst thereto, irradiating with 352-365nm ultraviolet light for 2-4h in a dark environment at a power of 40W, and then purifying the product with a chromatographic column to obtain carboxylated magnolol; Step 2: dissolving carboxylated magnolol and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt in an ethanol solvent, stirring at room temperature for 25-40 minutes, then adding N-hydroxysuccinimide, reacting in an ice-water bath for 1-2 hours, and then adding 10-13 ml of chitosan hydrochloride mixed solution, controlling the pH at 4-5.8 at room temperature, reacting for 20-24 hours, centrifuging the product, and washing it with ethanol, and finally dialyzing and freeze-drying to obtain magnolol-modified chitosan; Step 3: Add methanol solvent, 0.12-0.14 mmol of inhibitor dibutylhydroxytoluene, and 0.23-0.27 mmol of catalyst chloroplatinic acid in tetrahydrofuran solution into the reactor, stir for 2-4 minutes, continue to add 2.1-2.6 mmol of magnolol-modified chitosan and 1.9-2.2 mmol of silicone rubber, ultrasonically disperse for 30-40 minutes, react at 65-80°C for 4-6 hours, cool, centrifuge, and wash to obtain silicone rubber-modified chitosan.

3. The sterile liquid dressing according to claim 2, characterized in that: In the step 1, the molar ratio of magnolol, 3-mercaptopropionic acid and benzoin dimethyl ether catalyst is (1.3-1.4) mmol:1 mmol:(0.2-0.3) mmol.

4. The sterile liquid dressing according to claim 2, characterized in that: In the step 2, the molar ratio of carboxylated magnolol, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt, and N-hydroxysuccinimide is 1 mmol:(0.1-0.2) mmol:(0.1-0.3) mmol.

5. The sterile liquid dressing according to claim 1, characterized in that: The preparation method of the epoxy-modified quaternary ammonium boric acid is as follows: S1. Adding boric acid and 1,3-di(dimethylamino)-2-propanol to an acetone solvent, stirring and dispersing, reacting at 95-110° C. for 6-8 hours, and after the reaction, distilling under reduced pressure, filtering and drying to obtain a tertiary aminoboric acid; S2. Add tertiary amine boronic acid and 3-chloropropylene to N,N-dimethylformamide solvent, stir and disperse, react at 70-85° C. for 10-12 hours, and then distill under reduced pressure and filter to obtain alkenyl quaternary ammonium boronic acid; S3. At 25-30°C, add 8-12 mmol of olefin quaternary ammonium boric acid to N,N-dimethylformamide solvent, stir evenly, then add 40-55 mmol of aqueous hydrogen peroxide solution, stir and react for 3-5 hours. After the reaction is completed, let stand, distill under reduced pressure, and wash to obtain epoxy-modified quaternary ammonium boric acid.

6. The sterile liquid dressing according to claim 5, characterized in that: The molar ratio of boric acid to 1,3-bis(dimethylamino)-2-propanol in S1 is 1 mmol:(2.14-2.86) mmol.

7. The sterile liquid dressing according to claim 5, characterized in that: The molar ratio of tertiary amine boronic acid to 3-chloropropylene in S2 is 1 mmol:(3.5-4.2) mmol.

8. A method for preparing the sterile liquid dressing according to any one of claims 1 to 7, characterized in that: The preparation method of the sterile liquid dressing is as follows: EGF, silicone rubber modified chitosan, epoxy modified quaternary ammonium boric acid, polyacrylic resin IV, ethyl acetate, menthol, polyethylene glycol and purified water are added into a stirrer, and stirred at 20-30° C. for 3-5 minutes to obtain the sterile liquid dressing.

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