Magnetic bead mixing electromagnetic adsorption reaction device and reaction tube

Abstract

The invention provides a magnetic bead mixing electromagnetic adsorption reaction device and a reaction tube. The device is used for heating and mixing reagents in a reaction tube so that the reagents react fully. The device comprises: an upper mounting shell and a lower mounting shell stepped shell, an elastic buckle and a driving unit. The test tube comprises: a tube body, a grab ring groove and at least one buckle ring groove. By arranging an electromagnet, the reaction tube is heated and a magnetic field is generated, so that the magnetic beads in the reaction tube are adsorbed and the magnetic beads are fixed at the bottom of the test tube. When the positive and negative poles of the electromagnet are energized at high frequency, the direction of the magnetic field is switched at high frequency to improve the effect of mixing the magnetic beads. By arranging a temperature sensor, the heat generated by the electromagnet is monitored to achieve temperature control. By arranging a pneumatic joint, the pneumatic joint is connected to the outside world through an air pipe to bring out the gas in the gap between the inner cavity and the rotating body, and through the adiabatic expansion of the gas, the excessive heat generated by the electromagnet during the adjustment process is controlled.

Description

Technical Field

[0001] The invention relates to the technical field of mixing devices, in particular to a magnetic bead mixing electromagnetic adsorption reaction device and a reaction tube. Background Art

[0002] In the field of analytical methods for life sciences and clinical medical diagnosis, magnetic particles (or magnetic beads) with particle sizes ranging from submicron to tens of microns are often used as a key analytical reagent component to enrich and separate complex samples.

[0003] (such as blood, urine or other body fluids, etc.). Especially in clinical immunoassay instrument systems based on various chemiluminescence principles, surface functionalized magnetic particles and other reagents (such as labeled signal antibodies, biotinylated capture antibodies and functionalized antigens, etc.) become a separately packaged reagent component in the kit.

[0004] In a type of immunoassay called sandwich method, when these reagents are mixed with the sample to be tested (such as blood) in a set amount (volume × concentration) and order, and react at a certain temperature for a period of time, the analyte in the sample is captured on the magnetic particles by the antibodies on the surface of the magnetic particles, and further combines with the labeled signal antibody on the surface of the magnetic beads to form a so-called sandwich product, i.e., an immune complex, which is a combination of "capture antibody-target analyte-signal antibody" in sequence. This process is called the incubation process. After the incubation is completed, the paramagnetic properties of the magnetic beads are used, and under the action of an external magnetic field, the analyte enriched on the surface of the magnetic beads in the form of an immune complex can be separated from other components in the sample through magnetic separation and washing processes, thereby avoiding interference from other substances in the sample, such as red blood cells, white blood cells, platelets, bilirubin, etc., which exist in large quantities in the blood, and greatly improving the detection sensitivity.

[0005] Due to the effect of gravity, the micron-sized magnetic beads suspended in the reagent kit will gradually sink to the bottom of the reagent container after being left standing in the reagent kit, causing the pipette, such as a pipette gun, a pipette needle or a sampling needle, to be unable to obtain or unable to accurately obtain the set amount of magnetic beads, thereby causing erroneous analysis results. In the past instrument systems, there were two solutions to this problem: one is to use a mechanical stirring paddle to extend into the bottom of the container containing the magnetic beads before the pipette absorbs the magnetic beads each time to resuspend the magnetic beads to form a uniformly mixed magnetic bead suspension and absorb it immediately; the other is to keep the container containing the magnetic beads in a rotating or oscillating state continuously or intermittently during the entire process of the instrument being in the test working state to ensure that the magnetic bead suspension is always uniform during the instrument working state. The first method is used on the Elecsys and cobas series electrochemiluminescence immunoassay analyzers of Roche Diagnostics. Some other commercial systems use the second method, which generally uses a reciprocating rack and a gear on the outer wall of the magnetic bead container to drive the magnetic bead container to reciprocate, or the container containing the magnetic beads can be kept in a continuous or intermittent oscillation state to prevent the magnetic beads from settling or allow the settled particles to float again.

[0006] However, the strong shearing effect of the mechanical stirring paddle can easily destroy the functional substances on the surface of the magnetic beads, and the design and operation of the instrument become complicated, which increases the possibility of error. The reciprocating rack drives the magnetic bead container to rotate back and forth, which will form an uneven distribution of magnetic beads in the radial direction of the slender tubular container. At the same time, the fixed regular noise caused by the reciprocating friction between the rack and the gear is also unpleasant. The oscillation method is effective for mixing the suspension on the shallow surface, but it is not effective for mixing the sedimentation particles deep in the slender tubular container.

[0007] Therefore, how to maintain the homogeneity of the magnetic bead suspension placed in biological detection or medical diagnostic instruments without increasing the size and structural complexity of the detection instrument as much as possible has always been an unresolved problem in the field of biological detection or the design of fully automatic immunoassay instruments. Summary of the invention

[0008] In order to solve the above technical problems, one of the technical solutions adopted by the present invention is: a magnetic bead mixing electromagnetic adsorption reaction device, which is used to heat and mix the reagents in the reaction tube evenly so that the reagents react fully; the device comprises:

[0009] The upper mounting shell 1 and the lower mounting shell 2 are used to carry the components of the device; the upper mounting shell 1 and the lower mounting shell 2 are connected by a three-hole connector 3;

[0010] A stepped housing 4, which is located inside the upper mounting housing 1 and divides the upper mounting housing 1 into an inner cavity 5 and an outer cavity 6;

[0011] A rotating body 7, which is installed in the inner cavity 5 and the lower installation shell 2, and is used to rotate the reaction tube;

[0012] An elastic buckle 8, which is located at the upper end of the rotating body 7, and the reaction tube is installed and / or removed from the rotating body 7 by pressing the elastic buckle 8;

[0013] The driving unit 9 is used to provide power for the rotating body 7 , and the driving unit 9 is installed in the outer cavity 6 and the lower mounting shell 2 .

[0014] Furthermore, the driving unit 9 includes:

[0015] The driving motor 901 is installed in the outer cavity 6, and its driving end passes through the three-hole connector 3 and enters the lower mounting shell 2;

[0016] A driving gear 902 mounted on a driving end of the driving motor 901;

[0017] The driven gear 903 is fixedly mounted on the lower end of the rotating body 7 and meshes with the driving gear 902 .

[0018] Furthermore, the device also includes: an electromagnet 10, which is fixedly installed in the rotating body 7 and located below the reaction tube, so that the electromagnet 10 and the rotating body 7 rotate synchronously.

[0019] Further, when the electromagnet 10 is energized to generate heat, the reaction tube is heated;

[0020] When the electromagnet 10 is energized to generate a magnetic field, it has an adsorption effect on the magnetic beads in the reaction tube, fixing the magnetic beads at the bottom of the tube;

[0021] When the positive and negative poles of the electromagnet 10 are energized by high frequency conversion, the direction of the magnetic field is switched at high frequency, thereby improving the effect of mixing the magnetic beads.

[0022] Furthermore, the device also includes: a temperature sensor 11 for monitoring the heat generated by the electromagnet 10 to achieve temperature control;

[0023] The temperature sensor 11 is fixedly installed in the rotating body 7 and is located below the electromagnet 10 , so that the temperature sensor 11 rotates synchronously with the rotating body 7 .

[0024] Furthermore, the device also includes: an electric slip ring 12, which is used to transfer the circuit and signal on the rotating body 7 to avoid twisting caused by the rotation of the wire head;

[0025] The electric slip ring 12 is installed at the lower part of the rotating body 7 .

[0026] Furthermore, the device also includes: a pneumatic connector 13, which is installed in the outer cavity 6; the pneumatic connector 13 is connected to the outside through an air pipe, bringing out the gas in the gap between the inner cavity 5 and the rotating body 7, and through the adiabatic expansion of the gas, to control the excessive heat generated by the electromagnet 10 during the adjustment process.

[0027] Furthermore, there is at least one ball 14 between the rotating body 7 and the reaction tube, which is used to reliably fix the tube body 15 on the upper end of the rotating body 7 .

[0028] Another technical solution adopted by the present invention is: a reaction tube, which is applied to the above-mentioned magnetic bead mixing electromagnetic adsorption reaction device, and the test tube comprises:

[0029] A tube body 15, wherein the bottom of the tube body 15 is flat;

[0030] The grabbing ring groove 16 is used for grabbing or placing the test tube;

[0031] At least one buckle ring groove 17, used to cooperate with the ball of the elastic buckle 8 to fix the reaction tube;

[0032] The grabbing ring groove 16 and the buckling ring groove 17 are both arranged at the upper end of the tube body 15 , and the grabbing ring groove 16 is located above the buckling ring groove 17 .

[0033] Furthermore, the buckle ring groove 17 can be a discontinuous ring groove.

[0034] The advantages and positive effects of the present invention are as follows: by setting the electromagnet, when the electromagnet 10 is energized to generate heat, the reaction tube is heated; when the electromagnet is energized to generate a magnetic field, the magnetic beads in the reaction tube are adsorbed to fix the magnetic beads at the bottom of the tube; when the positive and negative poles of the electromagnet are energized at high frequency, the direction of the magnetic field is switched at high frequency, thereby improving the mixing effect of the magnetic beads;

[0035] Temperature control is achieved by setting a temperature sensor to monitor the heat generated by the electromagnet;

[0036] By setting a pneumatic joint, the pneumatic joint is connected to the outside through an air pipe, so as to bring out the gas in the gap between the inner cavity and the rotating body, and through the adiabatic expansion of the gas, the excessive heat generated by the electromagnet during the adjustment process is controlled. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 It is a schematic diagram of the structure of the magnetic bead mixing electromagnetic adsorption reaction device described in Example 1;

[0038] Figure 2 It is a structural schematic diagram of the magnetic bead mixing electromagnetic adsorption reaction device described in Example 2;

[0039] Figure 3This is a schematic diagram of the structure of the magnetic bead mixing electromagnetic adsorption reaction device described in Example 3;

[0040] Figure 4 This is a schematic diagram of the structure of the magnetic bead mixing electromagnetic adsorption reaction device described in Example 4;

[0041] Figure 5 This is a schematic diagram of the structure of the magnetic bead mixing electromagnetic adsorption reaction device described in Example 5;

[0042] Figure 6 Schematic diagram of the structure of the reaction tube;

[0043] In the figure: 1. upper mounting shell; 2. lower mounting shell; 3. three-hole connector; 4. stepped shell; 5. inner cavity; 6. outer cavity; 7. rotating body; 8. elastic buckle; 9. driving unit; 901. driving motor; 902. driving gear; 903. driven gear; 10. electromagnet; 11. temperature sensor; 12. electric slip ring; 13. pneumatic joint; 14. ball; 15. tube body; 16. grab ring groove; 17. buckle ring groove. DETAILED DESCRIPTION

[0044] In order to better understand the present invention, the present invention is further described below in conjunction with specific embodiments and drawings.

[0045] Example 1

[0046] like Figure 1 As shown, a magnetic bead mixing electromagnetic adsorption reaction device is used to heat and mix the reagents in the reaction tube evenly so that the reagents react fully; the device includes:

[0047] The upper mounting shell 1 and the lower mounting shell 2 are used to carry the components of the device; the upper mounting shell 1 and the lower mounting shell 2 are connected by a three-hole connector 3;

[0048] A stepped housing 4, which is located inside the upper mounting housing 1 and divides the upper mounting housing 1 into an inner cavity 5 and an outer cavity 6;

[0049] A rotating body 7, which is installed in the inner cavity 5 and the lower installation shell 2, and is used to rotate the reaction tube;

[0050] An elastic buckle 8, which is located at the upper end of the rotating body 7, and the reaction tube is installed and / or removed from the rotating body 7 by pressing the elastic buckle 8;

[0051] Furthermore, there is at least one ball 14 between the rotating body 7 and the reaction tube, which is used to reliably fix the tube body 15 on the upper end of the rotating body 7 .

[0052] The driving unit 9 is used to provide power for the rotating body 7 , and the driving unit 9 is installed in the outer cavity 6 and the lower mounting shell 2 .

[0053] Furthermore, the driving unit 9 includes:

[0054] The driving motor 901 is installed in the outer cavity 6, and its driving end passes through the three-hole connector 3 and enters the lower mounting shell 2;

[0055] A driving gear 902 mounted on a driving end of the driving motor 901;

[0056] The driven gear 903 is fixedly mounted on the lower end of the rotating body 7 and meshes with the driving gear 902 .

[0057] Specific working process: manually place the reaction tube on the rotating body 7, and stably clamp the reaction tube on the rotating body 7 through the elastic buckle 8, and then put the reaction tube into the rotating body 7. Magnetic beads and reaction liquid are placed in the tube, and the driving motor 901 is turned on. The driven gear 902 drives the driven gear 903 to rotate, and then drives the rotating body 7 to rotate, thereby realizing the rotation of the reaction tube and completing the full mixing of the reaction liquid in the reaction tube.

[0058] When the magnetic bead mixing electromagnetic adsorption reaction device is tilted at a certain angle, the motor 901 is driven to mix, and the mixing speed is controlled within the centrifugal acceleration a≤0.8g; the rotation speed during the mixing process can be adjusted to enhance the mixing effect.

[0059] Example 2

[0060] like Figure 2 As shown, based on Example 1, the device further includes: an electromagnet 10, which is fixedly installed in the rotating body 7 and located below the reaction tube, so that the electromagnet 10 and the rotating body 7 rotate synchronously.

[0061] Further, when the electromagnet 10 is energized to generate heat, the reaction tube is heated;

[0062] When the electromagnet 10 is energized to generate a magnetic field, it has an adsorption effect on the magnetic beads in the reaction tube, fixing the magnetic beads at the bottom of the tube;

[0063] When the positive and negative poles of the electromagnet 10 are energized by high frequency conversion, the direction of the magnetic field is switched at high frequency, thereby improving the effect of mixing the magnetic beads.

[0064] Example 3

[0065] like Figure 3 As shown, based on the second embodiment, the device further comprises: a temperature sensor 11, which is used to monitor the heat generated by the electromagnet 10 to achieve temperature control;

[0066] The temperature sensor 11 is fixedly installed in the rotating body 7 and is located below the electromagnet 10 , so that the temperature sensor 11 rotates synchronously with the rotating body 7 .

[0067] Example 4

[0068] like Figure 4 As shown, based on the third embodiment, the device further comprises: an electric slip ring 12, which is used to transfer the circuit and signal on the rotating body 7 to avoid the twisting phenomenon caused by the rotation of the wire head;

[0069] The electric slip ring 12 is installed at the lower part of the rotating body 7 .

[0070] Example 5

[0071] like Figure 5 As shown, further on the basis of Example 4, the device also includes: a pneumatic connector 13, which is installed in the outer cavity 6; the pneumatic connector 13 is connected to the outside through an air pipe, bringing out the gas in the gap between the inner cavity 5 and the rotating body 7, and through the adiabatic expansion of the gas, to control the excessive heat generated by the electromagnet 10 during the adjustment process.

[0072] Example 6

[0073] like Figure 6 As shown, a reaction tube is used in the magnetic bead mixing electromagnetic adsorption reaction device described in the above embodiments 1-5, and the test tube includes:

[0074] The tube body 15 has a flat bottom, which is used for facilitating installation with the electromagnet 10 .

[0075] At the same time, the mouth of the reaction tube can be deformed and designed to be a wide-mouth structure, which is convenient for liquid injection and discharge.

[0076] When the bottom of the tube body 15 is flat, a protrusion (rotating blade) can be added to the inner wall to increase the rotational force of the liquid during rotation;

[0077] In addition, a tightening thread can be added to the top of the reaction tube, and a corresponding functional cap unit can be installed and tightened to prepare for functional expansion.

[0078] The grabbing ring groove 16 is used for grabbing or placing the test tube;

[0079] At least one buckle ring groove 17, used to cooperate with the ball of the elastic buckle 8 to fix the reaction tube;

[0080] The grabbing ring groove 16 and the buckling ring groove 17 are both arranged at the upper end of the tube body 15 , and the grabbing ring groove 16 is located above the buckling ring groove 17 .

[0081] Furthermore, the buckle ring groove 17 may be an intermittent ring groove to facilitate the cap screwing function during automatic operation;

[0082] The embodiments of the present invention are described in detail above, but the contents described are only preferred embodiments of the present invention and cannot be considered to limit the scope of implementation of the present invention. All equivalent changes and improvements made within the scope of the present invention should still fall within the scope of this patent.

Claims

1. Magnetic bead mixing electromagnetic adsorption reaction device, characterized by: The device is used to heat and evenly mix the reagents in the reaction tube so that the reagents react fully; the device comprises: The upper mounting shell (1) and the lower mounting shell (2) are used to carry the components of the device; the upper mounting shell (1) and the lower mounting shell (2) are connected via a three-hole connecting piece (3); A stepped housing (4) is located inside the upper mounting housing (1) and divides the upper mounting housing (1) into an inner cavity (5) and an outer cavity (6); A rotating body (7), which is installed in the inner cavity (5) and the lower installation shell (2) and is used to rotate the reaction tube; An elastic buckle (8) is located at the upper end of the rotating body (7), and the reaction tube is installed and / or removed from the rotating body (7) by pressing the elastic buckle (8); A driving unit (9) is used to provide power for the rotating body (7), and the driving unit (9) is installed in the outer cavity (6) and the lower installation shell (2).

2. The magnetic bead mixing electromagnetic adsorption reaction device according to claim 1, characterized in that: The driving unit (9) comprises: A driving motor (901) is installed in the outer cavity (6), and its driving end passes through the three-hole connector (3) and enters the lower mounting housing (2); A driving gear (902) mounted on a driving end of the driving motor (901); The driven gear (903) is fixedly sleeved on the lower end of the rotating body (7) and meshes with the driving gear (902).

3. The magnetic bead mixing electromagnetic adsorption reaction device according to claim 1, characterized in that: The device further comprises an electromagnet (10), wherein the electromagnet (10) is fixedly mounted inside the rotating body (7) and located below the reaction test tube, so that the electromagnet (10) and the rotating body (7) rotate synchronously.

4. The magnetic bead mixing electromagnetic adsorption reaction device according to claim 3, characterized in that: When the electromagnet (10) is energized to generate heat, the reaction tube is heated; When the electromagnet (10) is energized to generate a magnetic field, it has an adsorption effect on the magnetic beads in the reaction tube, fixing the magnetic beads at the bottom of the tube; When the positive and negative poles of the electromagnet (10) are energized by high-frequency conversion, the direction of the magnetic field is switched at high frequency, thereby improving the effect of mixing the magnetic beads.

5. The magnetic bead mixing electromagnetic adsorption reaction device according to claim 4, characterized in that: The device further comprises: a temperature sensor (11) for monitoring the heat generated by the electromagnet (10) to achieve temperature control; The temperature sensor (11) is fixedly installed in the rotating body (7) and is located below the electromagnet (10), so that the temperature sensor (11) and the rotating body (7) rotate synchronously.

6. The magnetic bead mixing electromagnetic adsorption reaction device according to claim 1, characterized in that: The device also includes: an electric slip ring (12) for transferring the circuit and signal on the rotating body (7) to avoid twisting caused by the rotation of the wire head; The electric slip ring (12) is installed at the lower part of the rotating body (7).

7. The magnetic bead mixing electromagnetic adsorption reaction device according to claim 1, characterized in that: The device further comprises: a pneumatic joint (13) installed in the outer cavity (6); the pneumatic joint (13) is connected to the outside through an air pipe, takes out the gas in the gap between the inner cavity (5) and the rotating body (7), and controls the excessive heat generated by the electromagnet (10) during the adjustment process through the adiabatic expansion of the gas.

8. The magnetic bead mixing electromagnetic adsorption reaction device according to claim 1, characterized in that: At least one ball (14) is provided between the rotating body (7) and the reaction tube, and is used to reliably fix the reaction tube on the upper end of the rotating body (7).

9. A reaction tube, which is used in the magnetic bead mixing electromagnetic adsorption reaction device according to any one of claims 1 to 7, characterized in that: This tube includes: A tube body (15), wherein the bottom of the tube body (15) is flat; A grabbing ring groove (16) is used for grabbing or placing the test tube; At least one buckle ring groove (17) is used to cooperate with the ball of the elastic buckle (8) to fix the reaction tube; The grabbing ring groove (16) and the buckling ring groove (17) are both arranged at the upper end of the tube body (15), and the grabbing ring groove (16) is located above the buckling ring groove (17).

10. The reaction tube according to claim 1, characterized in that: The buckle ring groove (17) can be a discontinuous ring groove.

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