Valve type micro-fluidic chip

Abstract

The invention discloses a valve type microfluidic chip, which belongs to the field of microfluidics, and comprises a main body, the main body is provided with a storage area and a detection area, the main body is also provided with a first communication port and a second communication port, the first communication port is communicated with the storage area, the second communication port is communicated with the detection area, the valve type microfluidic chip also comprises a rotary valve, and the rotary valve is provided with a communication groove. The rotary valve is rotationally mounted on the main body, and when the rotary valve rotates to a first position, the communicating groove communicates with the first communicating port and the second communicating port, so that the storage area communicates with the detection area; when the rotary valve rotates to the second position, the communication groove is staggered from the first communication port and / or the second communication port, and the storage area is disconnected from the detection area, through the design, connection and disconnection of the storage area and the detection area can be controlled only by rotating the rotary valve, operation is easy, and the overall structure of the chip is simple.

Description

Technical Field

[0001] The invention relates to the field of microfluidics, in particular to a valve-type microfluidics chip. Background Art

[0002] The microfluidic chip analysis system mainly completes functions including sampling, dilution, injection, reaction, separation, analysis and detection in the chip system by manipulating the fluid in the microchannel. Researching microfluidic drive technology that is compatible with microchannels is the premise and basis for realizing microfluidic control. The drive and control of microfluids is very different from that of macrofluids, which is mainly due to the change in the flow characteristics of the fluid as the scale is reduced. This change in flow characteristics often makes the simple transplantation of macrofluid drive and control technology in microfluidics unsuccessful or ineffective, and the drive and control technology of microfluids is more complicated and diversified.

[0003] There are many types of microfluidic drive and control technologies, and the principles and forms adopted are different. They can be divided into pressure drive, electric drive, thermal drive, surface tension drive, centrifugal force drive, etc. Microvalve, as a fluid control component, can realize the opening and closing of fluid channels and the switching of fluid flow direction. It is one of the most important components in the microfluidic technology platform. With the rapid development of microfluidic chips, the chips will be able to undertake more and more reaction projects, and the liquid flow process in the chip will become more and more complicated. In this process, microvalves, as liquid flow direction switchers and fluid channel switches, need to shoulder more functions. The performance and manufacturing cost of microvalves will become one of the bottlenecks of microfluidic technology. The existing valve-type microfluidic chips have complex structures and cumbersome operations. Summary of the invention

[0004] In order to overcome the deficiencies of the prior art, one of the objectives of the present invention is to provide a valve-type microfluidic chip with a simple structure and convenient operation.

[0005] One of the purposes of the present invention is achieved by the following technical solution:

[0006] A valve-type microfluidic chip comprises a main body, wherein the main body is provided with a storage area and a detection area, and the main body is also provided with a first connecting port and a second connecting port, wherein the first connecting port is connected to the storage area, and the second connecting port is connected to the detection area. The valve-type microfluidic chip also comprises a rotary valve, wherein the rotary valve is provided with a connecting groove, and the rotary valve is rotatably installed on the main body, and when the rotary valve is rotated to a first position, the connecting groove is connected to the first connecting port and the second connecting port, so that the storage area is connected to the detection area; when the rotary valve is rotated to a second position, the connecting groove is staggered with the first connecting port and / or the second connecting port, and the storage area is disconnected from the detection area.

[0007] Furthermore, the valve-type microfluidic chip also includes a base, the base includes a plate body and a snap-on portion extending from the plate body, the plate body is fixed to the main body, the plate body is provided with a first through hole and a second through hole, the first through hole is connected to the first connecting port, the second through hole is connected to the second connecting port, the snap-on portion snaps with the rotary valve so that the rotary valve is rotatably installed on the base, and when the rotary valve is rotated to the first position, the connecting groove is connected to the first through hole and the second through hole.

[0008] Furthermore, the buckle portion is arc-shaped, and the rotary valve is located inside the buckle portion.

[0009] Furthermore, the first through hole and the second through hole are located in the buckle portion.

[0010] Furthermore, the base is made of transparent material.

[0011] Furthermore, the rotary valve includes a valve body and a gasket installed on the valve body, and the communicating groove is arranged on the gasket.

[0012] Furthermore, the gasket is made of silicone.

[0013] Furthermore, the main body is also provided with a waste liquid area and an exhaust hole connected to the waste liquid area, and the waste liquid area is connected to the detection area.

[0014] Furthermore, the main body is also provided with an injection port and a sample adding port, and the injection port and the sample adding port are respectively connected to the storage area.

[0015] Furthermore, the main body also includes a mixing block, and the mixing block is located in the detection area.

[0016] Compared with the prior art, the valve-type microfluidic chip of the present invention includes a main body, which is provided with a storage area and a detection area. The main body is also provided with a first connecting port and a second connecting port. The first connecting port is connected to the storage area, and the second connecting port is connected to the detection area. The valve-type microfluidic chip also includes a rotary valve, which is provided with a connecting groove. The rotary valve is rotatably installed on the main body. When the rotary valve is rotated to the first position, the connecting groove is connected to the first connecting port and the second connecting port, so that the storage area is connected to the detection area; when the rotary valve is rotated to the second position, the connecting groove is staggered with the first connecting port and / or the second connecting port, and the storage area is disconnected from the detection area. Through the above design, the connection and disconnection of the storage area and the detection area can be controlled by only rotating the rotary valve. The operation is simple and the overall structure of the chip is simple. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 A three-dimensional diagram of the valve-type microfluidic chip of the present invention;

[0018] Figure 2 for Figure 1 An exploded view of the valve-type microfluidic chip;

[0019] Figure 3 for Figure 1 A three-dimensional diagram of a base of a valve-type microfluidic chip;

[0020] Figure 4 for Figure 3 Another stereoscopic view of the base;

[0021] Figure 5 for Figure 1 A three-dimensional diagram of a rotary valve of a valve-type microfluidic chip;

[0022] Figure 6 It is a cross-sectional view of the valve-type microfluidic chip of the present invention in a disconnected state;

[0023] Figure 7 It is a cross-sectional view of the connected state of the valve-type microfluidic chip of the present invention.

[0024] In the figure: 10, main body; 11, bottom plate; 110, storage area; 111, detection area; 112, waste liquid area; 113, mixing block; 12, upper cover; 120, injection port; 121, sample adding port; 122, first connecting port; 123, second connecting port; 124, exhaust hole; 20, base; 21, plate body; 210, first through hole; 211, second through hole; 212, slide groove; 22, buckle part; 30, rotary valve; 31, valve body; 32, gasket; 320, connecting groove. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] It should be noted that when a component is referred to as being "fixed to" another component, it may be directly on the other component or there may be another intermediate component through which it is fixed. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may be another intermediate component at the same time. When a component is considered to be "set on" another component, it may be directly set on the other component or there may be another intermediate component at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0028] See also Figure 1 The valve-type microfluidic chip includes a main body 10, a base 20 and a rotary valve 30. The base 20 is fixed to the upper surface of the main body 10, and the rotary valve 30 is rotatably mounted on the base 20. The on-off of the flow channel in the main body 10 is controlled by rotating the rotary valve 30.

[0029] For details, please continue to refer to Figure 1 as well as Figure 2 The main body 10 is provided with a storage area 110, a detection area 111 and a waste liquid area 113. The storage area 110 is disconnected from the detection area 111, and the waste liquid area 113 is connected to the detection area 111. The detection area 111 is located between the storage area 110 and the waste liquid area 113. The main body 10 is also provided with an injection port 120, a sample adding port 121, a first communication port 122, a second communication port 123 and an exhaust hole 124. The injection port 120 and the sample adding port 121 are connected to the storage area 110, the first communication port 122 is connected to the storage area 110, and the second communication port 123 is connected to the detection area 111. The exhaust hole 124 is connected to the waste liquid area 113. The injection port 120 is used to inject reagents, the sample adding port 121 is used to add samples, and the exhaust hole 124 is convenient for exhausting the air in the detection area 111 to avoid bubbles in the sample liquid, and is also conducive to the rapid entry of the sample mixed liquid from the storage area 110 into the detection area 111, while preventing the sample from overflowing the chip from the exhaust hole 124. A mixing block 113 is provided in the detection area 111, and the number of the mixing blocks 113 is multiple, and the multiple mixing blocks 113 are staggered along the flow channel direction to facilitate liquid mixing.

[0030] The main body 10 is made of a transparent material, and any one of glass, PMMA, and PDMS is used. The transparent material has a high light transmittance, which is convenient for observation and obtaining high-quality images. The main body 10 includes a base plate 11 and an upper cover 12 fixedly connected to the base plate 11. The base plate 11 is recessed to form a storage area 110, a detection area 111, and a waste liquid area 113. The depths of the storage area 110 and the detection area 111 are 0.3-0.5mm. The depth of the waste liquid area 113 is 0.06-0.10mm. The injection port 120, the sample addition port 121, the first connecting port 122, the second connecting port 123, and the exhaust hole 124 are all arranged on the upper cover 12. The injection port 120 and the sample addition port 121 are located above the storage area 110 and at one end away from the detection area 111. The first connecting port 122 is located above the storage area 110 and at one end close to the detection area 111. The first connecting port 122 extends along the height direction of the main body 10. The second communication port 123 is located above the detection area 111 and close to one end of the storage area 110. The second communication port 123 extends along the height direction of the main body 10.

[0031] In other embodiments, the main body 10 may also be a three-layer structure, and the middle layer is provided with through grooves so that the upper layer, the middle layer and the lower layer form flow channels and different functional areas.

[0032] Please continue reading Figure 3 as well as Figure 4 The base 20 includes a plate body 21 and a buckle portion 22 extending from the upper surface of the plate body 21. There are two buckle portions 22, both of which are arc-shaped, and a buckle protrusion is provided on the top of each buckle portion 22. The plate body 21 is provided with a first through hole 210 and a second through hole 211, and the positions of the first through hole 210 and the second through hole 211 correspond to the first communication port 122 and the second communication port 123. The plate body 21 is also provided with a slide groove 212, and the slide groove 212 is used for the extension foot of the rotary valve 30 to rotate.

[0033] Please continue reading Figure 5 The rotary valve 30 includes a valve body 31 and a gasket 32 ​​mounted on the valve body 31. The gasket 32 ​​is recessed to form a connecting groove 320, and the connecting groove 320 is in a straight line shape. The connecting groove 320 is used to connect the storage area 110 and the detection area 111. The gasket 32 ​​is made of an elastic material to facilitate the sealing of the connecting groove 320. Specifically, the gasket 32 ​​is made of silicone.

[0034] Please continue reading Figure 6 as well as Figure 7 When assembling the valve type microfluidic chip, the plate body 21 of the base 20 is fixed to the upper cover 12 of the main body 10 , and at this time, the first through hole 210 is aligned with the first connecting port 122 , and the second through hole 211 is aligned with the second connecting port 123 .

[0035] When using a valve-type microfluidic chip, when the rotary valve 30 is closed (at this time, the connecting groove 320 is staggered with the first through hole 210 and / or the second through hole 211), the reagent is injected from the injection port 120 in advance, and then the injection port 120 and the sample addition port 121 are sealed with a sealing film, thereby realizing the pre-sealing of the reagent. When the chip is used, first, the gasket 32 ​​is rotated by rotating the rotary valve 30, so that the two ends of the connecting groove 320 are connected with the first through hole 210 and the second through hole 211 respectively. At this time, the valve is opened, and the storage area 110 and the detection area 111 are connected. When injecting liquid, the tip head is used to pierce the sealing film of the sample addition port 121 to inject the sample. The sample and the pre-sealed reagent enter the detection area 111 through the rotary valve 30 together, and the mixing operation is performed through the collision and diversion of the mixing block 113, and the excess liquid enters the waste liquid area 112.

[0036] Let’s take vaginal secretion microscopy as a specific usage scenario for a step-by-step introduction:

[0037] The reagent pre-sealed in the storage area 110 is a vaginal secretion staining liquid. When the rotary valve 30 is closed, the staining liquid is injected in advance from the injection port 120, and then the injection port 120 and the sample addition port 121 are sealed with a sealing film, thereby realizing the pre-sealing of the vaginal secretion staining liquid. During microscopic examination, the gasket 32 ​​is first rotated by rotating the rotary valve 30, so that the two ends of the connecting groove 320 at the bottom of the gasket 32 ​​are respectively connected with the first through hole 210 and the second through hole 211. At this time, the valve is opened, and the storage area 110 and the detection area 111 are connected. When injecting liquid, the tip head is used to pierce the sealing film of the sample addition port 121 to inject the vaginal secretion sample. The sample and the pre-sealed staining liquid enter the detection area 111 through the rotary valve 30 together, and the mixing and staining operation is performed after collision and diversion by the mixing block 113. The excess liquid enters the waste liquid area 112, and then the detection area 111 of the main body 10 is placed under a microscope for observation.

[0038] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which are equivalent modifications and improvements made to the above embodiments based on the essential technology of the present invention, and all of them belong to the protection scope of the present invention.

Claims

1. A valve-type microfluidic chip, comprising a main body, wherein the main body is provided with a storage area and a detection area, characterized in that: The main body is also provided with a first connecting port and a second connecting port, the first connecting port is connected to the storage area, and the second connecting port is connected to the detection area. The valve-type microfluidic chip also includes a rotary valve, the rotary valve is provided with a connecting groove, and the rotary valve is rotatably installed on the main body. When the rotary valve is rotated to a first position, the connecting groove is connected to the first connecting port and the second connecting port, so that the storage area is connected to the detection area; when the rotary valve is rotated to a second position, the connecting groove is staggered with the first connecting port and / or the second connecting port, and the storage area is disconnected from the detection area.

2. The valve-type microfluidic chip according to claim 1, characterized in that: The valve-type microfluidic chip also includes a base, which includes a plate body and a snap-on portion extending from the plate body, the plate body is fixed to the main body, the plate body is provided with a first through hole and a second through hole, the first through hole is connected to the first connecting port, the second through hole is connected to the second connecting port, the snap-on portion snaps with the rotary valve so that the rotary valve is rotatably installed on the base, and when the rotary valve is rotated to the first position, the connecting groove is connected to the first through hole and the second through hole.

3. The valve-type microfluidic chip according to claim 2, characterized in that: The buckle portion is arc-shaped, and the rotary valve is located inside the buckle portion.

4. The valve-type microfluidic chip according to claim 3, characterized in that: The first through hole and the second through hole are located in the buckle portion.

5. The valve-type microfluidic chip according to claim 2, characterized in that: The base is made of transparent material.

6. The valve-type microfluidic chip according to claim 1, characterized in that: The rotary valve comprises a valve body and a gasket installed on the valve body, and the communicating groove is arranged on the gasket.

7. The valve-type microfluidic chip according to claim 6, characterized in that: The gasket is made of silicone.

8. The valve-type microfluidic chip according to claim 1, characterized in that: The main body is also provided with a waste liquid area and an exhaust hole communicated with the waste liquid area, and the waste liquid area is communicated with the detection area.

9. The valve-type microfluidic chip according to claim 1, characterized in that: The main body is also provided with an injection port and a sample adding port, and the injection port and the sample adding port are respectively communicated with the storage area.

10. The valve-type microfluidic chip according to claim 1, characterized in that: The main body also includes a mixing block, and the mixing block is located in the detection area.

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