Method for carrying out biological grading refining by taking betel nut seed waste as raw material

Abstract

The invention relates to the technical field of betel nut recycling, and particularly discloses a method for performing biological grading refining by taking betel nut seed waste as a raw material, which is used for solving the problem that betel nuts cannot be recycled. Comprising the following steps that 1, betel nut seed waste is dried and smashed, and primary treatment is conducted; 2, carrying out steam explosion pretreatment on the betel nut seed waste subjected to primary treatment; 3, extracting, drying and concentrating alkaloid in the pretreated betel nut seed waste; 4, the extracted, dried and concentrated areca seed waste is extracted, and precipitates obtained after active small peptides are extracted are used as organic fertilizer raw materials. The areca seed waste is subjected to steam explosion pretreatment, alkaloid, the active small peptides and cellulose can be separated through the pretreatment, and the content of the active small peptides in the areca seed waste is reduced; therefore, the extraction rate of corresponding active substances in the subsequent treatment process is effectively improved, the extraction rate is greatly improved, the recovery cost is reduced, and the environmental pollution is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of betel nut recovery, in particular to a method for biological graded refining using betel nut seed waste as raw materials. Background Art

[0002] Due to the inherent characteristics of betel nut seed waste, the alkaloids, proteins, etc. in it are wrapped and combined by cellulose, so it is difficult to achieve a high extraction rate by using only simple chemical extraction methods. This directly leads to the difficulty in achieving economic benefits by extracting active substances from betel nut seed waste:

[0003] 1. The existing chemical extraction method requires a large amount of chemical solvents, resulting in high processing costs;

[0004] 2. The existing chemical extraction method will discharge a large amount of chemical pollutants during the deep processing of betel nut seed waste, causing serious environmental pollution. This runs counter to the purpose of turning betel nut seed waste into treasure.

[0005] Based on this, a method for bio-grading refining using betel nut seed waste as raw material is now provided, which can eliminate the drawbacks of existing devices. Summary of the invention

[0006] The purpose of the present invention is to provide a method for biological graded refining using betel nut seed waste as raw materials, thereby solving the problem that betel nut seeds cannot be reasonably utilized in the prior art.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A method for biologically fractionating and refining betel nut seed waste as raw material. The present invention provides a method for biologically fractionating and refining betel nut seed waste as raw material, the method comprising:

[0009] (1) Drying and crushing of betel nut seed waste;

[0010] (2) Steam explosion pretreatment of betel nut seed waste;

[0011] (3) Extraction and drying concentration of alkaloids from betel nut seed waste;

[0012] (4) Extraction and concentration of active oligosaccharides from betel nut seed waste;

[0013] (5) Extraction and concentration of active small peptides from betel nut seed waste;

[0014] (6) The precipitate after extracting the active peptide is used as the raw material of organic fertilizer and other processes;

[0015] The drying temperature of betel nut seed waste ranges from 60 to 70 degrees Celsius, the drying time ranges from 3 to 5 hours, and the moisture content after drying ranges from 15% to 20%.

[0016] The fineness of the betel nut seed waste after crushing ranges from 20 to 40 mesh;

[0017] Technical parameters of steam explosion pretreatment: pretreatment temperature range is 120 to 140 degrees Celsius, pressure range is 0.25 to 0.35 MPa, pressure holding time range is 10 to 20 minutes, and then steam is quickly released to rapidly reduce temperature and pressure.

[0018] When the betel nut seeds are subjected to the extraction treatment, the volume ratio of the betel nut seeds to the anhydrous ethanol ranges from 1:4 to 3:4, and the extraction time ranges from 20 minutes to 30 minutes;

[0019] The technical parameters for drying and concentrating the anhydrous ethanol extract of alkaloids in betel nut seeds are as follows: temperature range is 75 to 85 degrees Celsius, time range is 1.5 to 2.5 hours;

[0020] The volume ratio of the extracted betel nut seeds to the added water is in the range of 1:3 to 1:1, and the stirring and mixing time of the two is in the range of 120 to 180 seconds;

[0021] The technical parameters for extracting active oligosaccharides from the betel nut seeds after leaching are as follows: the amount of cellulose endonuclease added is 0.1% to 0.15% of the mass of the betel nut seeds, the enzymolysis reaction conditions are: the temperature is in the range of 35 to 45 degrees Celsius, and the insulation reaction time ranges from 20 to 30 minutes; the amount of cellulose exonuclease added is 0.15% to 0.2% of the mass of the betel nut seeds, and the enzymolysis reaction conditions are: the temperature is in the range of 40 to 50 degrees Celsius, and the insulation reaction time ranges from 30 to 40 minutes, and then the reaction solution is transported to a centrifugal assembly, the centrifugal speed ranges from 2500 to 3500 revolutions per minute, the centrifugal time ranges from 8 to 12 minutes, and the supernatant is dried and concentrated in the range of 75 to 85 degrees Celsius for 4 to 5 hours to obtain active oligosaccharides;

[0022] The volume ratio of betel nut seeds after active oligosaccharide extraction to water is in the range of 1:1 to 1:1.5, and the mixing time of the two is in the range of 120 seconds to 180 seconds;

[0023] The technical parameters for extracting active small peptides from betel nut seeds after extracting active oligosaccharides are as follows: the amount of protein endonuclease added is 0.05% to 1% of the mass of the betel nut seeds, the enzymatic reaction conditions are as follows: the temperature range is 40 to 50 degrees Celsius, the insulation reaction time range is 15 to 25 minutes, and then the reaction solution is transported to a centrifugal component, the centrifugal speed range is 3500 to 4500 revolutions per minute, the centrifugal time range is 10 to 15 minutes, and then the supernatant is taken and dried and concentrated in the range of 70 to 80 degrees Celsius for 5 to 6 hours to obtain active small peptides.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1. In view of the problem that it is difficult to achieve a high extraction rate in the prior art, the present invention performs steam explosion pretreatment on betel nut seed waste. Through the pretreatment, alkaloids, active small peptides and cellulose can be separated, thereby effectively improving the extraction rate of the corresponding active substances in the subsequent treatment process;

[0026] 2. In view of the problem that the prior art requires a large amount of chemical solvents, resulting in high costs, the present invention only uses anhydrous ethanol for extraction, and the anhydrous ethanol can be recycled after the extraction is completed, which can effectively reduce the cost required for extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a structural flow chart of the present invention. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] like Figure 1 As shown, the embodiment of the present invention provides a method for bio-grading refining using betel nut seed waste as raw material, comprising the following steps:

[0030] Step (1) collecting betel nut seed waste, drying at 65 degrees Celsius for 4 hours to reduce the moisture content to 15%, crushing to 20 meshes and then entering the next process;

[0031] Step (2) putting the crushed betel nut seeds into a steam explosion pretreatment chamber, closing the chamber door, heating the chamber to 120 degrees Celsius, controlling the pressure at about 0.25 MPa, maintaining the pressure for 15 minutes, and then quickly releasing the steam to rapidly reduce the temperature and pressure;

[0032] Step (3) After opening the door, take out the betel nut seeds that have been pre-treated by steam explosion, load them into the bio-grading refining reactor, and spray anhydrous ethanol from the upper part of the reactor at a mass ratio of 1:2 to extract the betel nut seeds. The extraction time is 20 minutes. Through this treatment, the alkaloids in the betel nut seeds are extracted by anhydrous ethanol and then flow out of the bio-grading refining reactor to obtain an alkaloid ethanol extract. The extract is dried and concentrated at 85 degrees Celsius for 1.5 hours to obtain alkaloids. The anhydrous ethanol evaporated during drying and concentration can be recovered and used for subsequent extraction;

[0033] Step (4) adding water in a ratio of 1:1.5 according to the volume of the extracted betel nut seeds, stirring for 120 seconds, adding 0.1% of endocellulose enzyme at a mass ratio, and then incubating at 40 degrees Celsius for 20 minutes, then adding 0.15% of exocellulose enzyme at a mass ratio, and then incubating at 45 degrees Celsius for 30 minutes, and then transferring the reaction solution to a centrifugal component, centrifuging at 3000 revolutions per minute for 10 minutes, and after solid-liquid separation, taking the supernatant, and drying and concentrating the supernatant containing active oligosaccharides at 85 degrees Celsius for 4.5 hours to obtain active oligosaccharides;

[0034] Step (5) transporting the precipitate treated in step (4) back to the bio-grading refining reactor, adding water in a ratio of 1:1.25 according to the volume of the precipitate, stirring for 180 seconds, adding protein endonuclease in a mass ratio of 0.05%, keeping warm at 40 degrees Celsius for 15 minutes, and then transporting the reaction liquid to the centrifuge assembly, centrifuging the reaction liquid at 4000 revolutions per minute for 10 minutes, and after solid-liquid separation, taking the supernatant, drying and concentrating the supernatant containing the active small peptide at 80 degrees Celsius for 5 hours to obtain the active small peptide;

[0035] Step (6) The precipitate treated in step (5) can be used as an organic fertilizer raw material;

[0036] Step (7) uses the method to perform biological fractionation refining on betel nut seed waste, which can effectively extract active oligosaccharides and active small peptides from betel nut seeds, and can also efficiently extract alkaloids with pharmacological effects from betel nut seeds. In addition, the invention has a reduction effect on the increasingly serious solid waste and has good ecological and environmental benefits.

[0037] The present invention aims to solve the problem that it is difficult to achieve a high extraction rate in the prior art. The present invention performs steam explosion pretreatment on betel nut seed waste. Through the pretreatment, alkaloids, active small peptides and cellulose can be separated, thereby effectively improving the extraction rate of corresponding active substances in subsequent treatment processes, thereby greatly improving the extraction rate;

[0038] In view of the problem that the existing technology needs to consume a large amount of chemical solvents, resulting in high costs, the present invention only uses anhydrous ethanol for extraction, and the anhydrous ethanol can be recycled after the extraction is completed, which can effectively reduce the cost required for extraction;

[0039] In view of the problem that the existing production method will discharge a large amount of chemical pollutants and cause serious environmental pollution during the deep processing of betel nut seed waste, the extraction process of the present invention adopts a variety of microbial enzyme preparations as environmentally friendly catalysts, and the by-products produced after the final solid-liquid separation can be used as organic fertilizer raw materials, without causing new pollution to the environment.

[0040] The implementation of the present invention can effectively utilize betel nut seed waste and play a role in eliminating environmental pollution.

[0041] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for bio-grading and refining using betel nut seed waste as raw material, characterized in that: The following steps are involved: Step 1: Drying and crushing the betel nut seed waste for preliminary treatment; Step 2: pre-treating the betel nut seed waste after preliminary treatment by steam explosion; Step 3: extracting and drying the alkaloids in the pretreated betel nut seed waste; Step 4: Extract the leached and dried concentrated betel nut seed waste, and use the precipitate after extracting the active small peptides as an organic fertilizer raw material.

2. The method for bio-grading refining using betel nut seed waste as raw material according to claim 1, characterized in that: The drying temperature of betel nut seed waste ranges from 60 to 70 degrees Celsius, the drying time ranges from 3 to 5 hours, and the moisture content after drying ranges from 15% to 20%.

3. The method for bio-grading refining using betel nut seed waste as raw material according to claim 1, characterized in that: The betel nut seed waste after crushing has a fineness ranging from 20 to 40 meshes.

4. The method for bio-grading refining using betel nut seed waste as raw material according to claim 1, characterized in that: Technical parameters of steam explosion pretreatment: pretreatment temperature range is 120 to 140 degrees Celsius, pressure range is 0.25 to 0.35 MPa, pressure holding time range is 10 to 20 minutes, and then steam is quickly released to rapidly reduce temperature and pressure.

5. The method for bio-grading and refining using betel nut seed waste as raw material according to claim 1, characterized in that: When the betel nut seeds are extracted, the volume ratio of the betel nut seeds to anhydrous ethanol ranges from 1:4 to 3:4, and the extraction time ranges from 20 minutes to 30 minutes.

6. The method for bio-grading refining using betel nut seed waste as raw material according to claim 1, characterized in that: The volume ratio of the extracted betel nut seeds to the added water is in the range of 1:3 to 1:1, and the stirring and mixing time of the two is in the range of 120 to 180 seconds.

7. The method for bio-grading and refining using betel nut seed waste as raw material according to claim 1, characterized in that: The technical parameters for extracting active oligosaccharides from betel nut seeds after leaching are as follows: the amount of cellulose endo-enzyme added is 0.1% to 0.15% of the mass of the betel nut seeds, the enzymatic reaction conditions are: the temperature is in the range of 35 to 45 degrees Celsius, and the insulation reaction time is in the range of 20 to 30 minutes; the amount of cellulose exo-enzyme added is 0.15% to 0.2% of the mass of the betel nut seeds, and the enzymatic reaction conditions are: the temperature is in the range of 40 to 50 degrees Celsius, and the insulation reaction time is in the range of 30 to 40 minutes; then the reaction liquid is transported to a centrifugal assembly, the centrifugal speed ranges from 2500 to 3500 revolutions per minute, the centrifugal time ranges from 8 to 12 minutes, and the supernatant is dried and concentrated in the range of 75 to 85 degrees Celsius for 4 to 5 hours to obtain active oligosaccharides.

8. The method for bio-grading and refining using betel nut seed waste as raw material according to claim 7, characterized in that: The volume ratio of betel nut seeds added to water after active oligosaccharides are in the range of 1:1 to 1:1.5, and the mixing time of the two is in the range of 120 seconds to 180 seconds.

9. The method for bio-grading and refining using betel nut seed waste as raw material according to claim 7, characterized in that: The technical parameters for extracting active small peptides from betel nut seeds after extracting active oligosaccharides are as follows: the amount of protein endonuclease added is 0.05% to 1% of the mass of the betel nut seeds, the enzymatic reaction conditions are as follows: the temperature range is 40 to 50 degrees Celsius, the insulation reaction time range is 15 to 25 minutes, and then the reaction solution is transported to a centrifugal component, the centrifugal speed range is 3500 to 4500 revolutions per minute, the centrifugal time range is 10 to 15 minutes, and then the supernatant is taken and dried and concentrated in the range of 70 to 80 degrees Celsius for 5 to 6 hours to obtain active small peptides.

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