Lignin microbiological degradation amplification reactor

Abstract

The invention belongs to the technical field of lignin degradation, and particularly relates to a lignin microbiological degradation amplification reactor which comprises a fermentation assembly and a feeding assembly, the fermentation assembly is used for providing a large-capacity oxygen-enriched container for lignin degradation, and the feeding assembly is used for filling lignin, distilled water and seed liquid into reaction flasks in the fermentation assembly in batches according to the proportion. The double-layer reaction bottle formed by the plastic film in the fermentation assembly can continuously convey clean air rich in oxygen into the interlayer between the inner wall and the outer wall through the air supply pipe at the bottom of the double-layer reaction bottle; oxygen-enriched clean air entering the interlayer enters a fermentation reaction mixed solution in the reaction bottle through first vent holes densely distributed in the inner wall of the interlayer so as to provide enough required oxygen for the fermentation reaction in the reaction bottle, so that the fermentation mixed solution in the reaction bottle is fully fermented; the fermentation reaction efficiency and the fermentation quality of the fermentation mixed liquid in the reaction bottle are effectively improved.

Description

Technical Field

[0001] The invention belongs to the technical field of lignin degradation, and in particular relates to a lignin microbial degradation amplification reactor. Background Art

[0002] Lignin (Latin, English, German: Lignin) is a class of complex organic polymers that form important structural materials in the supporting tissues of vascular plants and some algae. Lignins are particularly important in the formation of cell walls, especially in wood and bark, because they impart rigidity and resistance to decay. Chemically, lignins are cross-linked phenolic polymers. The xylem of plants (a structure responsible for transporting water and minerals) contains a large amount of lignin, which allows the xylem to maintain its high rigidity and support the weight of the entire plant.

[0003] Lignin can be degraded by microorganisms, and white rot fungi are common microorganisms used to degrade lignin. The suitable degradation temperature of white rot fungi is 20 degrees to 30 degrees. White rot fungi are aerobic microorganisms, and the degradation process of lignin by white rot fungi does not require light.

[0004] The degradation rate of lignin by white rot fungi is slow and the time is long. The high degradation efficiency achieved in degradation experiments in small containers under laboratory environment and conditions is very difficult to maintain the same degradation efficiency in the same environment and conditions in large industrial reactors. Generally, special large reaction vessels are required for efficient degradation design and experiments. That is, the transition from small experimental containers to large industrial containers requires a lot of work.

[0005] In addition, although the environment and conditions for degradation using white rot fungi have been greatly improved, the degradation cycle still needs to be measured in weeks, which is still relatively long and the efficiency is relatively low. At present, most people use fermentation degradation tanks to achieve degradation, that is, a large number of degradation tanks are required during the cycle of large-scale lignin degradation, resulting in higher degradation costs.

[0006] The present invention designs a lignin microbial degradation amplification reactor to solve the above problems. Summary of the invention

[0007] Based on this, it is necessary to provide a lignin microbial degradation amplification reactor to address the problems existing in the current lignin degradation reactor. The fermentation component in the present invention utilizes 75 small-capacity reaction bottles evenly divided into three layers to form an overall large-capacity fermentation reaction container. While maintaining the laboratory research environment and conditions of the small container, the transformation of small-capacity container fermentation to large-capacity container fermentation is effectively completed, so that the overall large-capacity reactor can effectively ferment lignin under the same environment and conditions as the laboratory research. There is no need to carry out a large amount of additional experimental work on the transformation of laboratory small-container fermentation to large-container fermentation specifically for the required fermentation environment and conditions of the large-capacity reactor. The suitable and favorable fermentation environment and conditions that can be quickly achieved in the laboratory are quickly applied to the actual large-container fermentation and degradation, effectively improving the efficiency of the lignin fermentation and degradation experiment. The double-layer reaction bottle formed by the plastic film in the fermentation component of the present invention can continuously deliver oxygen-rich clean air to the interlayer between its inner wall and outer wall through the air delivery pipe at the bottom thereof, and the oxygen-rich clean air entering the interlayer enters the fermentation reaction mixture in the reaction bottle through the first air vents densely distributed on its inner wall to provide sufficient required oxygen for the fermentation reaction in the reaction bottle, so that the fermentation mixture in the reaction bottle is fully fermented, and the fermentation reaction efficiency and fermentation quality of the fermentation mixture in the reaction bottle are effectively improved. The fermentation components of the present invention can be combined in groups of three after completing the filling of the fermentation mixture of all the reaction bottles, so that the reaction bottles in the three combined fermentation components are distributed up and down, thereby effectively saving the space occupied by the fermentation components after all the reaction bottles are filled with the fermentation mixture and pushed into the warehouse to wait for the fermentation process, so that more fermentation components can be placed in the warehouse and lignin can be effectively fermented and degraded in large quantities, effectively improving the efficiency of the lignin fermentation reaction. In the present invention, the film sleeves provided on the outside of the fermentation components formed in groups of three can slow down the loss of oxygen-rich clean air released from the reaction bottles, so that all the reaction bottles in the three fermentation components are always in an oxygen-rich clean environment during the fermentation reaction of the fermentation mixture, preventing the fermentation mixture in the open reaction bottles from being contaminated, improving the fermentation and degradation reaction efficiency of the fermentation mixture in the reaction bottles, and improving the product quality after the fermentation and degradation reaction. The reaction bottles in the fermentation components of the present invention are formed of plastic films, which effectively reduces the cost of a large number of large-capacity reaction vessels required for the large-capacity fermentation and degradation reaction of lignin. The first addition component, the second addition component and the third addition component in the feeding component of the present invention can achieve the purpose of quickly filling the fermentation mixture into all the reaction bottles in the fermentation component, effectively improving the filling efficiency.

[0008] The above purpose is achieved through the following technical solutions: A lignin microbial degradation amplification reactor, used for lignin degradation, comprises: A fermentation component is used to provide a large-capacity oxygen-enriched container for lignin degradation. The fermentation component includes a first frame with three layers of compact space, a pair of horizontally through slots are opened on the bottom side walls of the first frame, and cross bars are horizontally slid in both ends of the slots. The exposed ends of the cross bars are provided with vertically retractable multi-section telescopic legs, and the telescopic sections of the multi-section telescopic legs are threadedly connected with locking screws that cooperate with the inner telescopic sections. Casters are provided at the lower ends of the multi-section telescopic legs. 25 pairs of vertical rods with hooks at the lower ends are arranged in an array on the top of each layer of the first frame, and a reaction bottle made of a polymer film is hooked at the lower end of each pair of the vertical rods. The bottle wall of the reaction bottle consists of an inner wall and an outer wall forming a sealed interlayer, and an air supply pipe connected to the interlayer is provided at the bottom of the outer wall, and the inner wall is densely covered with first air vents with an inner diameter smaller than the diameter of the lignin particles.

[0009] A feeding component is used for batch filling of lignin, distilled water and seed liquid into the reaction bottles in the fermentation component in proportion, and the feeding component includes a second frame with three layers of compact space, and each layer of the second frame is provided with five first adding components for quantitatively filling seed liquid into the reaction bottles, five second adding components for quantitatively adding lignin into the reaction bottles, and five third adding components for quantitatively filling distilled water into the reaction bottles.

[0010] In one of the embodiments, a first baffle is provided at the bottom of the first frame to seal it, a film sleeve of polymer material is provided on the outer side of the first frame, two hanging ears cooperating with the hooks at the lower ends of the corresponding two vertical rods are symmetrically provided on the outer side of the upper bottle mouth of the reaction bottle, and the locking screws cooperating with the cross bar are threadedly connected on the wall surface of the slot, and the bottom air supply pipes of all the reaction bottles in the fermentation assembly are connected to the first main pipe through the pipe network, and the first main pipe is connected to the pipe network joint by plugging.

[0011] In one of the embodiments, the first adding component includes a horizontal first feeding tube fixed to the second frame, a first discharge port is provided on the tube wall at one end of the first feeding tube, a first discharge pipe for filling the reaction bottle with seed liquid is arranged at the first discharge port, a liquid discharge groove connected to the first discharge port is provided on the inner side of the tube wall between the first discharge port and the end of the first feeding tube, a first driving rod is slidably arranged in the circular hole at the other end of the first feeding tube, a first piston and a second piston are axially spaced apart and arranged at the inner end of the first driving rod, and the first piston is located between the circular hole at the end of the first feeding tube and the first piston. The first piston is provided with a first stopper and a second piston, the axial thickness of the second piston is smaller than the distance between the first discharge port and the exposed end of the first feeding pipe, a first feed port and a first air hole for facilitating the movement of the first piston are provided on the tube wall of the first feeding pipe, the first feed port is connected to the first feeding pipe through a first valve assembly cooperating with the first piston, the first valve assembly opens to the first after the first piston moves from the first discharge port to the first feeding port and passes through the feeding pipe, and closes to the first feeding pipe after the first piston passes through the first feeding port in the opposite direction, and a first limiting ring for limiting the first piston is provided in the first feeding pipe.

[0012] In one embodiment, the first valve assembly includes a cylindrical shell, which is arranged between the first feed port and the first feed pipe and is connected, the axis of the cylindrical shell is perpendicular to the axis of the first feed pipe, a cylindrical first valve plug is sealed and rotated in the cylindrical shell, a through second feed port is provided on the cylindrical surface of the first valve plug, two first vortex springs are connected between the two end pins of the first valve plug and the cylindrical shell, a shift block cooperating with the first piston is provided on the cylindrical surface of the first valve plug, a limiting block is provided on the end face of the first valve plug, and the limiting block moves in the limiting groove on the inner wall of the cylindrical shell.

[0013] In one of the embodiments, all the first feeding pipes on the first feeding pipes are connected to the first liquid pump on the bottom partition of the second frame through a second main pipe arranged on the second frame, and the first driving rods in all the first feeding pipes are synchronously connected through a first connecting frame arranged in the second frame. A horizontal rack is arranged on the first connecting frame, and the rack slides in two first guide sleeves on the second frame. The second frame is provided with a motor, and a gear meshing with the rack is arranged on the output shaft of the motor.

[0014] In one embodiment, the second adding component includes a cylindrical first silo arranged in a second frame, a third feed port and a second discharge port are opened at one end of the first silo, a second feed pipe and a second discharge pipe for adding lignin particles into the reaction bottle are respectively arranged at the third feed port and the second discharge port, a second valve assembly and a third valve assembly are respectively arranged in the second feed pipe and the second discharge pipe, the second valve assembly and the third valve assembly have the same structure, the second valve assembly and the third valve assembly are respectively in an open state and a closed state when a negative pressure is generated in the first silo, and are respectively in a closed state and an open state when a positive pressure is generated in the first silo, the second feed pipe is connected to the adsorption port through a fourth main pipe, an outer shell forming a sandwich therewith is arranged on the outer side of the first silo, second vent holes connected to the outer shell are evenly and densely distributed on the wall surface of the first silo, an air pipe connected to the inner shell is arranged at the end of the outer shell, and the air pipe is connected to the air pump on the second frame through the third main pipe.

[0015] In one embodiment, the second valve assembly or the third valve assembly includes a second baffle plate that cooperates with the upper limit strip on the inner wall of the second feed pipe or the second discharge pipe, the second baffle plate is hinged to the support ear on the inner wall of the second feed pipe or the second discharge pipe through a hinge shaft, a second vortex spring is connected between the hinge shaft and the support ear, the swing end of the second baffle plate is provided with a sealing gasket that cooperates with the inner wall of the second feed pipe or the second discharge pipe, and the support ear is provided with a sealing gasket that cooperates with the second baffle plate.

[0016] In one embodiment, the third adding component includes a cylindrical second material bin arranged in the second frame, a fourth feed port and a third discharge port are opened at one end of the second material bin, a third feed pipe and a third discharge pipe for filling distilled water into the reaction bottle are respectively arranged at the fourth feed port and the third discharge port, a fourth valve assembly and a fifth valve assembly are respectively arranged in the third feed pipe and the third discharge pipe, the fourth valve assembly has the same structure as the fifth valve assembly, and the fourth valve assembly and the fifth valve assembly are respectively in an open state and a closed state when a negative pressure is generated in the second material bin, and are respectively in a closed state when a positive pressure is generated in the second material bin. and open state, the third feed pipe is connected with the second liquid pump on the second frame through the fifth main pipe, the discharge end of the third discharge pipe is provided with a sixth valve assembly, the sixth valve assembly is opened when the fifth valve assembly is opened and is closed when the fifth valve assembly is closed, a third piston and two second limiting rings that limit the movement amplitude of the third piston are slidably arranged in the second material bin, a second air hole that facilitates the movement of the third piston is opened on the wall surface of the second material bin, a second driving rod fixedly connected with a third bolt is slidably arranged in the circular hole at the other end of the second material bin, and the second driving rod is fixedly connected to a second connecting frame in the second frame body that is horizontally driven by an electric push rod.

[0017] In one embodiment, the fourth valve assembly or the fifth valve assembly includes a second guide sleeve, which is fixed in a chamber on the inner wall of a third feed pipe or a third discharge pipe by four circumferentially evenly distributed fixing rods, a first guide rod is slidably arranged in the second guide sleeve, a second valve plug for switching the third feed pipe or the third discharge pipe is arranged at one end of the first guide rod, and a first spring is connected between the second valve plug and the fixing rod to drive the second valve plug to close the third feed pipe or the third discharge pipe.

[0018] In one embodiment, the sixth valve assembly includes a spherical shell arranged at the discharge end of the third feed pipe or the third discharge pipe, the wall surface of the spherical shell is provided with a first ring sleeve opposite to the port of the third feed pipe or the third discharge pipe, the first ring sleeve is threadedly connected with a screw sleeve, a cross is provided in the screw sleeve, a second guide rod is slidably provided in the circular hole in the middle of the cross, the inner end of the second guide rod is provided with a spherical third valve plug for switching the port of the third feed pipe or the third discharge pipe, and a second spring is connected between the third valve plug and the cross to drive the third valve plug to close the port of the third feed pipe or the third discharge pipe.

[0019] The beneficial effects of the present invention are: 1. The fermentation component of the present invention utilizes 75 small-capacity reaction bottles evenly divided into three layers to form an overall large-capacity fermentation reaction container. While maintaining the laboratory research environment and conditions of the small container, the conversion of small-capacity container fermentation to large-capacity container fermentation is effectively completed, so that the overall large-capacity reactor can effectively ferment lignin under the same environment and conditions as the laboratory research. There is no need to carry out a large amount of additional experimental work on the conversion of laboratory small-container fermentation to large-container fermentation specifically for the required fermentation environment and conditions of the large-capacity reactor. The suitable and favorable fermentation environment and conditions that can be quickly achieved in the laboratory are quickly applied to the actual large-container fermentation and degradation, effectively improving the efficiency of the lignin fermentation and degradation experiment.

[0020] 2. The double-layer reaction bottle formed by the plastic film in the fermentation assembly of the present invention can continuously transport oxygen-rich clean air to the interlayer between its inner wall and outer wall through the air supply pipe at the bottom thereof. The oxygen-rich clean air entering the interlayer enters the fermentation reaction mixture in the reaction bottle through the first air holes densely distributed on its inner wall, thereby providing sufficient oxygen required for the fermentation reaction in the reaction bottle, so that the fermentation mixture in the reaction bottle can be fully fermented, thereby effectively improving the fermentation reaction efficiency and fermentation quality of the fermentation mixture in the reaction bottle.

[0021] 3. After all the reaction bottles are filled with the fermentation mixture, the fermentation components of the present invention can be combined in groups of three, so that the reaction bottles in the three combined fermentation components are distributed up and down, thereby effectively saving the space occupied by the fermentation components when all the reaction bottles are pushed into the warehouse to wait for the fermentation process after the fermentation mixture is filled. More fermentation components can be placed in the warehouse and effective large-capacity fermentation and degradation reactions of lignin can be carried out, effectively improving the efficiency of the lignin fermentation and degradation reaction.

[0022] 4. In the present invention, the film sleeves provided on the outside of the fermentation components formed in groups of three can slow down the loss of oxygen-rich clean air released from the reaction bottles, so that all the reaction bottles in the three fermentation components are always in an oxygen-rich clean environment during the fermentation reaction of the fermentation mixed liquid therein, preventing the fermentation mixed liquid in the open reaction bottles from being contaminated, improving the fermentation degradation reaction efficiency of the fermentation mixed liquid in the reaction bottles, and improving the quality of the products after the fermentation degradation reaction.

[0023] 5. The reaction bottle in the fermentation assembly of the present invention is formed of a plastic film, which effectively reduces the cost of a large number of large-capacity reaction vessels required for large-capacity fermentation and degradation reactions of lignin.

[0024] 6. The first adding assembly, the second adding assembly and the third adding assembly in the feeding assembly of the present invention can achieve the purpose of quickly filling the fermentation mixture into all the reaction bottles in the fermentation assembly, effectively improving the filling efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 are two schematic diagrams of the combination of three fermentation components; Figure 2 are two overall cross-sectional views of the fermentation component assembly; Figure 3 are two schematic diagrams of fermentation components; Figure 4 It is a schematic diagram of the first frame structure in the fermentation component; Figure 5 It is a partial cross-sectional view of the multi-section telescopic legs in the fermentation assembly; Figure 6 It is a partial cross-sectional view of the cooperation between the multi-section telescopic legs and the first frame in the fermentation assembly; Figure 7 It is a schematic diagram of the arrangement structure of the reaction bottles in the fermentation assembly; Figure 8 is the reaction bottle and its cross-sectional view; Fig. 9 It is a cross-sectional view of the reaction bottle; Fig.10 is a schematic diagram of the feeding assembly; Fig.11 is a cross-sectional view of the charging assembly; Fig.12 It is a cross-sectional view of the arrangement structure of the first adding component, the second adding component and the third adding component in the adding component; Fig.13 It is a schematic diagram of the distribution of the first adding component in the adding component; Fig.14 It is the first added component and its two partial cutaway views; Fig.15 It is a perspective view of the cooperation between the first piston and the shifting block on the first valve plug in the first adding component; Fig.16 is a cross-sectional view of a first valve assembly in a first additive assembly; Fig.17 is a schematic structural diagram of a first valve plug in a first valve assembly; Fig.18 is a schematic diagram of the distribution of the second adding component in the adding component; Fig.19 are two cross-sectional views of the second added component; Fig. 20 is a partial cross-sectional view of the second addition assembly in the addition assembly; Fig.21 is a longitudinal sectional view of the second valve assembly and the third valve assembly in the second additional assembly; Fig. 22 is a cross-sectional view of the second valve assembly and the third valve assembly in the second additional assembly; Fig.23 It is a schematic diagram of the distribution of the third adding component in the adding component; Fig.24 are three cross-sectional views of the third added component; Fig.25 It is a cross-sectional view of the cooperation between the fermentation component and the feeding component; Fig.26 It is a cross-sectional view of a single set of the first adding component, the second adding component and the third adding component in cooperation with the reaction bottle; Name of the label in the figure: 100, fermentation assembly; 101, first frame; 102, slot; 103, vertical rod; 104, hook; 105, first baffle; 106, multi-section telescopic legs; 107, telescopic joint; 108, locking screw; 109, cross bar; 110, caster; 111, reaction bottle; 112, outer wall; 113, air supply pipe; 114, inner wall; 115, first vent hole; 116, hanging ear; 117, first main pipe; 118, membrane sleeve; 200, feeding assembly; 201, second frame; 202, partition; 300, first adding assembly; 301, first feeding pipe; 302, first air hole; 303, first discharge port; 304, drainage groove; 305, first discharge pipe; 306, first feed port; 307, cylindrical shell; 308, limiter groove; 309, first valve plug; 310, second feed port; 311, rotating pin; 312, limiter block; 313, first vortex spring; 314, shifter block; 315, first limiter ring; 316, first driving rod; 317, first piston; 318, second piston; 319, first connecting frame; 320, first feed pipe; 321, second main pipe; 322, first liquid pump; 324, rack; 325, first guide sleeve; 326, gear; 327, motor; 328, first valve assembly; 400, second adding component; 401, first silo; 402, second vent hole; 403, third feed port; 404, second discharge port; 405, second feed pipe; 406, second discharge pipe; 407, second valve assembly; 408, third valve assembly; 409, second baffle; 410, hinge shaft; 411, lug; 412, second vortex spring; 413, sealing pad; 414, housing; 415, air pipe; 416, third main pipe; 417, air pump; 418, fourth main pipe; 419, limit strip; 500, third adding component; 501, second silo; 502, second air hole; 503, fourth feed port; 504, third discharge port; 505, third feed pipe; 506, third discharge pipe; 507, fourth valve assembly; 508, fifth valve assembly; 509, first guide rod; 510, second valve plug; 511, first spring; 512, spherical shell; 513, first ring sleeve; 514, screw sleeve; 515, cross; 516, second guide rod; 517, third valve plug; 518, second spring; 519, sixth valve assembly; 520, second limiting ring; 521, second driving rod; 522, third piston; 523, second connecting frame; 524, electric push rod; 525, fifth main pipe; 526, second liquid pump; 527, second guide sleeve; 528, fixing rod; 529, chamber. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below through embodiments and in conjunction with the accompanying drawings. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0027] The serial numbers assigned to the components herein, such as "first", "second", etc., are only used to distinguish the objects described and do not have any order or technical meaning. The "connection" and "coupling" mentioned in this application, unless otherwise specified, include direct and indirect connections (couplings). In the description of the present invention, it should be understood that the orientation or position relationship indicated by the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", etc. are based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present invention.

[0028] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0029] like Figure 1-Figure 26 As shown, a lignin microbial degradation amplification reactor is used for lignin degradation, comprising: The fermentation assembly 100 is used to provide a large-capacity oxygen-enriched container for lignin degradation. The fermentation assembly 100 includes a first frame 101 with three layers of compact space. The bottom side wall of the first frame 101 is provided with a pair of horizontally penetrating slots 102. Both ends of the slots 102 are provided with horizontally sliding cross bars 109. The exposed ends of the cross bars 109 are provided with vertically telescopic multi-section telescopic legs 106. The telescopic sections 107 of the multi-section telescopic legs 106 are threadedly connected with locking screws 108 that cooperate with the inner telescopic sections 107. A caster 110 is provided at the lower end of the multi-section telescopic legs 106, and 25 pairs of vertical rods 103 with hooks 104 at the lower ends are arranged in an array on the top of each layer of the first frame 101. A reaction bottle 111 made of a polymer film is hooked at the lower end of each pair of the vertical rods 103. The bottle wall of the reaction bottle 111 consists of an inner wall 114 and an outer wall 112 that form a sealed interlayer. An air supply pipe 415113 connected to the interlayer is provided at the bottom of the outer wall 112, and the inner wall 114 is densely covered with first air vents 115 with an inner diameter smaller than the diameter of the lignin particles.

[0030] The feeding component 200 is used for batch filling of lignin, distilled water and seed liquid into the reaction bottle 111 in the fermentation component 100 in proportion. The feeding component 200 includes a second frame 201 with three layers of compact space, and each layer of the second frame 201 is provided with five first adding components 300 for quantitatively filling seed liquid into the reaction bottle 111, five second adding components 400 for quantitatively adding lignin into the reaction bottle 111, and five third adding components 500 for quantitatively filling distilled water into the reaction bottle 111.

[0031] In a further embodiment, Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 6 , Figure 7 , Figure 8 , Fig.26 As shown, a first baffle 105 is provided at the bottom of the first frame 101 to seal it, a film sleeve 118 of a polymer material is provided on the outer side of the first frame 101, two ears 116 cooperating with the hooks 104 at the lower ends of the two corresponding vertical rods 103 are symmetrically provided on the outer side of the upper bottle mouth of the reaction bottle 111, and the locking screws 108 cooperating with the cross bar 109 are threadedly connected on the wall surface of the slot 102, and the bottom air supply pipes 415113 of all the reaction bottles 111 in the fermentation component 100 are connected to the first main pipe 117 through the pipe network, and the first main pipe 117 is connected to the pipe network joint by plugging.

[0032] In a further embodiment, Fig.10 , Fig.13 , Fig.14As shown, the first adding component 300 includes a horizontal first feeding tube 301 fixed to the second frame 201, a first discharge port 303 is provided on the tube wall at one end of the first feeding tube 301, a first discharge port 305 for filling the reaction bottle 111 with seed liquid is arranged at the first discharge port 303, a liquid discharge groove 304 connected to the first discharge port 303 is provided on the inner side of the tube wall between the first discharge port 303 and the end of the first feeding tube 301, a first driving rod 316 is slidably arranged in the circular hole at the other end of the first feeding tube 301, and a first piston 317 and a second piston 318 are axially spaced and arranged at the inner end of the first driving rod 316, and the first piston 317 is located between the circular hole at the end of the first feeding tube 301 and the second piston 318. 18, the axial thickness of the second piston 318 is smaller than the distance between the first discharge port 303 and the exposed end of the first feeding pipe 301, and the first feeding port 306 and the first air hole 302 for facilitating the movement of the first piston 317 are opened on the tube wall of the first feeding pipe 301, the first feeding port 306 is connected to the first feeding pipe 320 through a first valve assembly 328 cooperating with the first piston 317, the first valve assembly 328 opens to the first after the first piston 317 moves from the first discharge port 303 to the first feeding port and passes through the feeding pipe, and closes the first feeding pipe 320 after the first piston 317 passes through the first feeding port in the opposite direction, and a first limiting ring 315 for limiting the first piston 317 is arranged in the first feeding pipe 301.

[0033] In a further embodiment, Fig.14 , Fig.15 , Fig.16 , Fig.17 As shown, the first valve assembly 328 includes a cylindrical shell 307, and the cylindrical shell 307 is arranged between the first feed port 306 and the first feed pipe 320 and is connected. The axis of the cylindrical shell 307 is perpendicular to the axis of the first feeding pipe 301. A cylindrical first valve plug 309 is sealed and rotated in the cylindrical shell 307, and a through second feed port 310 is opened on the cylindrical surface of the first valve plug 309. Two first vortex springs 313 are connected between the two end pins 311 of the first valve plug 309 and the cylindrical shell 307. A shift block 314 cooperating with the first piston 317 is arranged on the cylindrical surface of the first valve plug 309, and a limiting block 312 is arranged on the end face of the first valve plug 309. The limiting block 312 moves in the limiting groove 308 of the inner wall 114 of the cylindrical shell 307.

[0034] In a further embodiment, Fig.10 , Fig.11 , Fig.12 , Fig.13 , Fig.14As shown, all the first feeding pipes 320 on the first feeding pipes 301 are connected to the first liquid pump 322 on the bottom partition 202 of the second frame 201 through the second main pipe 321 arranged on the second frame 201, and the first driving rods 316 in all the first feeding pipes 301 are synchronously connected through the first connecting frame 319 arranged in the second frame 201, and the first connecting frame 319 is provided with a horizontal rack 324, and the rack 324 slides in the two first guide sleeves 325 on the second frame 201, and the second frame 201 is provided with a motor 327, and the output shaft of the motor 327 is provided with a gear 326 meshing with the rack 324.

[0035] In a further embodiment, Fig.10 , Fig.18 , Fig.19 , Fig. 20 As shown, the second adding component 400 includes a cylindrical first silo 401 arranged in the second frame 201, and one end of the first silo 401 is provided with a third feed port 403 and a second discharge port 404, and the third feed port 403 and the second discharge port 404 are respectively provided with a second feed pipe 405 and a second discharge pipe 406 for adding lignin particles into the reaction bottle 111, and the second feed pipe 405 and the second discharge pipe 406 are respectively provided with a second valve assembly 407 and a third valve assembly 408, and the second valve assembly 407 and the third valve assembly 408 have the same structure, and the second valve assembly 407 and the third valve assembly 408 are respectively provided. The valve assembly 408 is in an open state and a closed state respectively when negative pressure is generated in the first material bin 401, and is in a closed state and an open state respectively when positive pressure is generated in the first material bin 401. The second feed pipe 405 is connected to the adsorption port through the fourth main pipe 418. The outer side of the first material bin 401 is provided with an outer shell 414 forming a sandwich therewith. The wall surface of the first material bin 401 is evenly and densely distributed with second vents 402 connected with the outer shell 414. The end of the outer shell 414 is provided with an air pipe 415 connected with the inner shell. The air pipe 415 is connected with the air pump 417 on the second frame 201 through the third main pipe 416.

[0036] In a further embodiment, Fig.21 , Fig. 22As shown, the second valve assembly 407 or the third valve assembly 408 includes a second baffle 409 that cooperates with the upper limit strip 419 on the inner wall 114 of the second feed pipe 405 or the second discharge pipe 406, the second baffle 409 is hinged to the ear 411 of the inner wall 114 of the second feed pipe 405 or the second discharge pipe 406 through a hinge shaft 410, a second vortex spring 412 is connected between the hinge shaft 410 and the ear 411, a sealing gasket 413 that cooperates with the inner wall 114 of the second feed pipe 405 or the second discharge pipe 406 is provided at the swing end of the second baffle 409, and a sealing gasket 413 that cooperates with the second baffle 409 is provided on the ear 411.

[0037] In a further embodiment, Fig.10 , Fig.23 , Fig.24 As shown, the third adding component 500 includes a cylindrical second material bin 501 arranged in the second frame 201, and a fourth feed port 503 and a third discharge port 504 are opened at one end of the second material bin 501, and a third feed pipe 505 and a third discharge pipe 506 for filling distilled water into the reaction bottle 111 are respectively arranged at the fourth feed port 503 and the third discharge port 504, and a fourth valve assembly 507 and a fifth valve assembly 508 are respectively arranged in the third feed pipe 505 and the third discharge pipe 506, and the fourth valve assembly 507 has the same structure as the fifth valve assembly 508, and the fourth valve assembly 507 and the fifth valve assembly 508 are respectively in an open state and a closed state when a negative pressure is generated in the second material bin 501, and are respectively in a closed state and an open state when a positive pressure is generated in the second material bin 501. The third feed pipe 505 is connected to the second liquid pump 526 on the second frame 201 through the fifth main pipe 525, and the discharge end of the third discharge pipe 506 is provided with a sixth valve assembly 519, which is opened when the fifth valve assembly 508 is opened and closed when the fifth valve assembly 508 is closed. A third piston 522 and two second limit rings 520 that limit the movement of the third piston 522 are slidably arranged in the second material bin 501, and a second air hole 502 that facilitates the movement of the third piston 522 is opened on the wall surface of the second material bin 501. A second driving rod 521 fixedly connected with a third bolt is slidably arranged in the circular hole at the other end of the second material bin 501, and the second driving rod 521 is fixedly connected to a second connecting frame 523 in the second frame 201 that is horizontally driven by an electric push rod 524.

[0038] In a further embodiment, Fig.24As shown, the fourth valve assembly 507 or the fifth valve assembly 508 includes a second guide sleeve 527, and the second guide sleeve 527 is fixed in a chamber 529 of an inner wall 114 of the third feed pipe 505 or the third discharge pipe 506 by four circumferentially evenly distributed fixing rods 528, a first guide rod 509 is slidably arranged in the second guide sleeve 527, a second valve plug 510 for switching the third feed pipe 505 or the third discharge pipe 506 is arranged at one end of the first guide rod 509, and a first spring 511 for driving the second valve plug 510 to close the third feed pipe 505 or the third discharge pipe 506 is connected between the second valve plug 510 and the fixing rod 528.

[0039] In a further embodiment, Fig.24 As shown, the sixth valve assembly 519 includes a spherical shell 512 arranged at the discharge end of the third feed pipe 505 or the third discharge pipe 506, the wall of the spherical shell 512 is provided with a first ring sleeve 513 opposite to the port of the third feed pipe 505 or the third discharge pipe 506, the first ring sleeve 513 is threadedly connected with a screw sleeve 514, a cross 515 is arranged in the screw sleeve 514, a second guide rod 516 is slidably arranged in the circular hole in the middle of the cross 515, the inner end of the second guide rod 516 is provided with a spherical third valve plug 517 for switching the port of the third feed pipe 505 or the third discharge pipe 506, and a second spring 518 is connected between the third valve plug 517 and the cross 515 to drive the third valve plug 517 to close the port of the third feed pipe 505 or the third discharge pipe 506.

[0040] The fermentation component 100 in the present invention utilizes 75 small-capacity reaction bottles 111 evenly divided into three layers to form an overall large-capacity fermentation reaction container. While maintaining the laboratory research environment and conditions of the small container, the conversion of small-capacity container fermentation to large-capacity container fermentation is effectively completed, so that the overall large-capacity reactor can effectively ferment lignin under the same environment and conditions as the laboratory research. There is no need to carry out a large amount of additional experimental work on the conversion of laboratory small-container fermentation to large-container fermentation specifically for the required fermentation environment and conditions of the large-capacity reactor. The suitable and favorable fermentation environment and conditions that can be quickly achieved in the laboratory are quickly applied to the actual large-container fermentation and degradation, effectively improving the efficiency of the lignin fermentation and degradation experiment. The double-layer reaction bottle 111 formed by a plastic film in the fermentation component 100 of the present invention can continuously transport oxygen-rich clean air to the interlayer between its inner wall 114 and the outer wall 112 through the air supply pipe 415113 at the bottom thereof. The oxygen-rich clean air entering the interlayer enters the fermentation reaction mixture in the reaction bottle 111 through the first air vents 115 densely distributed on the inner wall 114 thereof, thereby providing sufficient oxygen required for the fermentation reaction in the reaction bottle 111, so that the fermentation mixture in the reaction bottle 111 is fully fermented, thereby effectively improving the fermentation reaction efficiency and fermentation quality of the fermentation mixture in the reaction bottle 111. The fermentation components 100 of the present invention can be combined in groups of three after completing the filling of the fermentation mixture of all the reaction bottles 111, so that the reaction bottles 111 in the three fermentation components 100 formed are distributed up and down, thereby effectively saving the space occupied by the fermentation components 100 in which all the reaction bottles 111 are pushed into the warehouse to wait for the fermentation process after completing the filling of the fermentation mixture, so that more fermentation components 100 can be placed in the warehouse and the lignin can be effectively fermented and degraded in large quantities, effectively improving the efficiency of the lignin fermentation reaction. In the present invention, the film sleeve 118 provided on the outside of the fermentation components 100 combined in groups of three can slow down the loss of oxygen-rich clean air released from the reaction bottles 111, so that all the reaction bottles 111 in the three fermentation components 100 are always in an oxygen-rich clean environment during the fermentation reaction of the fermentation mixture therein, preventing the fermentation mixture in the open reaction bottles 111 from being contaminated, improving the fermentation and degradation reaction efficiency of the fermentation mixture in the reaction bottles 111, and improving the product quality after the fermentation and degradation reaction. The reaction bottles 111 in the fermentation assembly 100 of the present invention are formed of plastic film, which effectively reduces the cost of a large number of large-capacity reaction vessels required for large-capacity fermentation and degradation reactions of lignin. The first adding assembly 300, the second adding assembly 400 and the third adding assembly 500 in the feeding assembly 200 of the present invention can achieve the purpose of quickly filling the fermentation mixture into all the reaction bottles 111 in the fermentation assembly 100, effectively improving the filling efficiency.

[0041] The operation process of the present invention is as follows: In the initial state, three layers of reaction bottles 111 are placed in the first frame 101 of each fermentation assembly 100. The reaction bottles 111 are hung on the hooks 104 at the lower ends of the two vertical rods 103 at the top of the corresponding layer through the upper hanging ears 116. The first main pipe 117 connected to the air supply pipe 415113 at the bottom of the reaction bottle 111 is in a closed state. Fig.15 As shown, the first valve assemblies 328 of all the first adding assemblies 300 in the adding assembly 200 are in an open state, the limiting block 312 on the first valve plug 309 is located at one end of the corresponding limiting groove 308, the first vortex spring 313 is in a compressed state, the first piston 317 and the second piston 318 in each first adding assembly 300 are located at the corresponding first feed port 306 and are respectively located on both sides of the first feed port 306, the first piston 317 is in a toggling state with the shifting block 314 on the first valve plug 309, the shifting block 314 is against the cylindrical surface of the first piston 317, and the seed liquid in the first feed pipe 320 fills the space between the first piston 317 and the second piston 318 through the opened first valve assembly 328. The first silo 401 in the second adding component 400 contains lignin particles, the second valve component 407 and the third valve component 408 are both in a closed state, the second vortex springs 412 in the second valve component 407 and the third valve component 408 are both in a compressed state, and the second baffles 409 in the second valve component 407 and the third valve component 408 are abutted against the corresponding limit strips 419 under the action of the corresponding second vortex springs 412 and close the second feed pipe 405 and the second discharge pipe 406 respectively. The space on the side of the fourth feed port 503 of the third piston 522 in the second material bin 501 of the third adding component 500 and the third discharge pipe 506 are filled with distilled water, the third piston 522 abuts against the second limiting ring 520 in the second material bin 501 near the fourth feed port 503, the fourth valve assembly 507 and the fifth valve assembly 508 are in a closed state, the second valve plug 510 in the fourth valve assembly 507 and the fifth valve assembly 508 are in a closed state to the third feed pipe 505 and the third discharge pipe 506 respectively, the first spring 511 in the fourth valve assembly 507 and the fifth valve assembly 508 is in a compressed state, and the third valve plug 517 in the sixth valve assembly 519 is in a closed state to the discharge port of the third discharge pipe 506 under the action of the compressed second spring 518.

[0042] When the present invention is needed to carry out large-capacity lignin fermentation and degradation, first adjust the length of the multi-section telescopic legs 106 in the fermentation component 100 to an appropriate length so that the three-layer reaction bottle 111 in the fermentation component 100 matches the height of the three-layer fifteen groups of the first adding component 300, the second adding component 400 and the third adding component 500 in the feeding component 200 respectively, loosen the locking screws 108 between the telescopic sections 107 on the multi-section telescopic legs 106, so that the length of the multi-section telescopic legs 106 is changed to a height that matches the height of the feeding component 200, and then tighten the locking screws 108 to lock the length of the multi-section telescopic legs 106.

[0043] After the length adjustment of the four multi-section telescopic legs 106 in the fermentation component 100 is completed, the fermentation component 100 is pushed to the side of the feeding component 200, so that the discharge ports of the three layers and fifteen groups of the first adding component 300, the second adding component 400 and the third adding component 500 in the feeding component 200 are first respectively opposite to the bottle openings of the three layers and fifteen reaction bottles 111 closest to the second frame 201 in the fermentation component 100.

[0044] Then, the motor 327 is started, and the motor 327 drives the first driving rods 316 in all the first adding components 300 through the gear 326, the rack 324 and the first connecting frame 319 to synchronously push the first piston 317 and the second piston 318 to move toward the first discharge port 303. The first piston 317 and the second piston 318 push the quantitative seed liquid between the two toward the first discharge port 303. When the second piston 318 reaches the end of the first feeding tube 301, the seed liquid between the first piston 317 and the second piston 318 reaches the first discharge tube 305 through the first discharge port 303 and the drainage groove 304 and is added into the reaction bottle 111.

[0045] During the movement of the first piston 317 and the second piston 318 toward the first discharge port 303, the first valve plug 309 in the first valve assembly 328 rotates and gradually closes the first feed pipe 320 under the action of the corresponding first vortex spring 313. When the first piston 317 passes over the first feed port 306, the restriction of the first piston 317 on the shift block 314 on the first valve plug 309 is released, and the first valve plug 309 completely closes the first feed pipe 320 under the action of the first vortex spring 313.

[0046] After the seed liquid between the first piston 317 and the second piston 318 is completely added into the reaction bottle 111, the motor 327 is started in reverse. The motor 327 drives the first driving rods 316 in all the first adding components 300 to reset through a series of transmissions. The first driving rods 316 drive the corresponding first pistons 317 and second pistons 318 to reset. When the first piston 317 reaches the first feed port 306, the side end face of the first limiting ring 315 of the first piston 317 first meets the upper end face of the first valve plug 309, and the first valve plug 309 rotates and resets under the action of the shifting block 314 during the resetting process of the first piston 317 and compresses the first vortex spring 313 again, and finally the first feed pipe 320 is closed as the first piston 317 is reset.

[0047] After the first piston 317 and the second piston 318 in the first adding component 300 are reset, the first liquid pump 322 is started, and the second main pipe 321 of the first liquid pump 322, the first feed pipe 320, the second feed port 310 on the first valve plug 309 of the first valve assembly 328, and the first feed port 306 refill the space between the first piston 317 and the second piston 318 with seed liquid.

[0048] At the same time, the air pump 417 is started, and the air pump 417 forms a negative pressure in the interlayer between the first silo 401 and the shell 414 in all the second adding components 400, and then forms a negative pressure in the first silo 401 through the second vent hole 402 on the wall of the first silo 401. The second baffle 409 of the second valve assembly 407 in the second feeding pipe 405 overcomes the corresponding second vortex spring 412 to open under the action of the negative pressure, and the suction nozzle at the port of the fourth main pipe 418 moves the lignin particles through the second vent hole 402 on the wall of the first silo 401. The second feed pipe 405 enters the first silo 401. The negative pressure in the first silo 401 will cause the second baffle 409 of the third valve assembly 408 in the second discharge pipe 406 to close the second discharge pipe 406 under the action of the corresponding second vortex spring 412 without leakage. The lignin particles in the sender's first silo 401 gradually fill the first silo 401. Since the aperture of the second vent 402 is smaller than the diameter of the lignin particles, the lignin will not leak into the outer shell 414 through the second vent 402. When the first silo 401 is full of lignin particles, the lignin particles block all the second air holes 402, and the air pump 417 runs in reverse to deliver air into the outer shell 414, thereby forming a positive pressure in the first silo 401. The second valve assembly 407 in the second feed pipe 405 closes the second feed pipe 405 under the combined action of the positive pressure and the corresponding second vortex spring 412 to prevent the material from leaking in the opposite direction. The second baffle 409 of the third valve assembly 408 in the second discharge pipe 406 overcomes the elastic force of the corresponding second vortex spring 412 under the action of the positive pressure and opens the second discharge pipe 406. The lignin particles in the first silo 401 are all quantitatively added into the reaction bottle 111 through the opened second discharge pipe 406.

[0049] During the process of the second adding component 400 adding lignin to the reaction bottle 111, the second liquid pump 526 and the electric push rod 524 are started at the same time. The electric push rod 524 drives all the second driving rods 521 in the third adding component 500 to move synchronously toward the outside of the second silo 501 through the second connecting frame 523. The second driving rods 521 drive the corresponding third pistons 522 to move synchronously. The second liquid pump 526 and the fifth main pipe 525 pump distilled water into the third feeding pipe 505. The second valve plug 510 in the fourth valve assembly 507 in the third feeding pipe 505 opens the third feeding pipe 505 under the combined action of the negative pressure in the second silo 501 and the distilled water in the third feeding pipe 505 and further compresses the corresponding first spring 511. The distilled water in the third feeding pipe 505 enters the second silo 501 and fills the second silo 501 when the third piston 522 reaches the second limiting ring 520 on the side of the second air hole 502.

[0050] After the second material bin 501 is full, the second liquid pump 526 is stopped and the electric push rod 524 is started in reverse. The electric push rod 524 drives the third piston 522 to perform a reset movement through a series of transmissions. The second valve plug 510 in the fourth valve assembly 507 in the third feed pipe 505 closes the third feed pipe 505 under the corresponding first spring 511 and the water pressure in the second material bin 501 to prevent the distilled water from leaking in the opposite direction. The second valve plug 510 of the fifth valve assembly 508 in the third discharge pipe 506 opens the third discharge pipe 506 under the action of the water pressure in the second material bin 501 and further compresses the corresponding first spring 511. The distilled water in the second material bin 501 reaches the sixth valve assembly 519 through the third discharge pipe 506 and overcomes the elastic force of the second spring 518 of the sixth valve assembly 519 to push open the third valve plug 517 to complete the quantitative filling of distilled water into the reaction bottle 111.

[0051] After the quantitative filling of lignin, distilled water and seed liquid in the fifteen reaction bottles 111 on the first side and three layers in the fermentation component 100 is completed, the fermentation component 100 is moved to the second frame 201 again, so that the bottle mouths of the fifteen reaction bottles 111 on the second side and three layers in the fermentation component 100 correspond one by one to the three layers and fifteen groups of the first adding component 300, the second adding component 400 and the third adding component 500 in the adding component 200. Then, the above process is installed to complete the material filling of the fifteen reaction bottles 111 on the second side and three layers again. In this way, the quantitative filling of lignin, distilled water and seed liquid in all the reaction bottles 111 in the fermentation component 100 is completed in sequence.

[0052] like Figure 1 , Figure 2 , Figure 3As shown, after all the reaction bottles 111 in all the fermentation components 100 have completed the quantitative filling of lignin, distilled water and seed liquid, all the fermentation components 100 are grouped into three groups, and the lengths of the three multi-section telescopic legs 106 in each group are adjusted to the longest, the middle and the shortest, respectively, so that the top of the first frame 101 corresponding to the middle-length multi-section telescopic leg 106 is slightly lower than the bottom of the first frame 101 corresponding to the longest multi-section telescopic leg 106, and the shortest multi-section telescopic leg 106 is slightly lower than the bottom of the first frame 101 corresponding to the longest multi-section telescopic leg 106. 06 The top of the corresponding first frame 101 is slightly lower than the bottom of the middle and long multi-section telescopic legs 106. At the same time, the spacing between the two pairs of multi-section telescopic legs 106 in each fermentation component 100 in each group is adjusted so that the overall width of the two pairs of middle and long multi-section telescopic legs 106 in each group is slightly smaller than the spacing between the two pairs of the longest multi-section telescopic legs 106 in the corresponding group, and the overall width of the two pairs of the shortest multi-section telescopic legs 106 in each group is slightly smaller than the spacing between the two pairs of middle and long telescopic legs in the corresponding group.

[0053] Then, if Figure 2 As shown, the fermentation assembly 100 of the medium-length multi-section telescopic legs 106 in each group is pushed laterally under the first frame 101 of the longest multi-section telescopic legs 106 in the corresponding group, and the fermentation assembly 100 corresponding to the shortest multi-section telescopic legs 106 is pushed under the first frame 101 of the medium-length multi-section telescopic legs 106 in the corresponding group, thereby completing the upper and lower combination and merging of the three fermentation assemblies 100 in each group, thereby saving the space they occupy in the warehouse.

[0054] Next, a membrane sleeve 118 is provided on the outside of each group of three fermentation assemblies 100 that have been merged, so that a gap is formed between the lower edge of the membrane sleeve 118 and the first baffle 105 at the bottom of the first frame 101 in the fermentation assembly 100 corresponding to the shortest multi-section telescopic legs 106 in the corresponding group of fermentation assemblies 100, so as to avoid the subsequent rapid leakage of oxygen-rich clean air in the membrane sleeve 118 and ensure that the reaction bottle 111 in the membrane sleeve 118 is in an oxygen-rich and clean environment.

[0055] After each group completes the installation of the membrane sleeve 118, oxygen-rich clean air is transported to the interlayer of each reaction bottle 111 through the first main pipe 117 of the three fermentation components 100 in each group via the air supply pipe 415113 at the bottom of each reaction bottle 111. The air entering the interlayer of the reaction bottle 111 enters the reaction liquid through the first air vent 115 on the inner wall 114. The oxygen-rich clean air squeezed into the reaction liquid stirs the material while transporting oxygen inward, thereby improving the degradation reaction efficiency of the material in the reaction bottle 111.

[0056] The air entering the reaction bottle 111 enters the film sleeve 118 through the bottle mouth, so that a clean environment surrounding the reaction bottle 111 is formed in the film sleeve 118, which is conducive to the reaction bottle 111 to perform a fully and effectively degradation reaction in a sterile environment.

Claims

1. A lignin microbial degradation amplification reactor for lignin degradation, characterized in that: include: A fermentation component, used for providing a large-capacity oxygen-enriched container for lignin degradation, the fermentation component comprises a first frame body with three layers of compact space, a pair of horizontally penetrating slots are opened on the bottom side wall of the first frame body, cross bars are horizontally slid in both ends of the slots, and the exposed ends of the cross bars are provided with vertically retractable multi-section telescopic legs, the telescopic sections of the multi-section telescopic legs are threadedly connected with locking screws that cooperate with the inner telescopic sections, and the lower ends of the multi-section telescopic legs are provided with casters, 25 pairs of vertical rods with hooks at the lower ends are arranged in an array on the top of each layer of the first frame body, and a reaction bottle made of a polymer film is hooked at the lower end of each pair of the vertical rods, the bottle wall of the reaction bottle is composed of an inner wall and an outer wall forming a sealed interlayer, an air supply pipe connected to the interlayer is provided at the bottom of the outer wall, and the inner wall is densely covered with first ventilation holes with an inner diameter smaller than the diameter of the lignin particles; A feeding component is used for batch filling of lignin, distilled water and seed liquid into the reaction bottles in the fermentation component in proportion, and the feeding component includes a second frame with three layers of compact space, and each layer of the second frame is provided with five first adding components for quantitatively filling seed liquid into the reaction bottles, five second adding components for quantitatively adding lignin into the reaction bottles, and five third adding components for quantitatively filling distilled water into the reaction bottles.

2. The lignin microbial degradation amplification reactor according to claim 1, characterized in that: A first baffle is provided at the bottom of the first frame to seal it, a film sleeve of polymer material is provided on the outer side of the first frame, two hanging ears cooperating with the hooks at the lower ends of the two corresponding vertical rods are symmetrically provided on the outer side of the upper bottle mouth of the reaction bottle, and locking screws cooperating with the cross bar are threadedly connected on the wall surface of the slot, and the bottom air supply pipes of all reaction bottles in the fermentation assembly are connected to the first main pipe through the pipe network, and the first main pipe is connected to the pipe network joint by plugging.

3. The amplified lignin degradation reactor of claim 1, characterized in that: The first adding component includes a horizontal first feeding tube fixed to the second frame, a first discharge port is provided on the tube wall at one end of the first feeding tube, a first discharge pipe for filling the reaction bottle with seed liquid is arranged at the first discharge port, a liquid discharge groove connected with the first discharge port is provided on the inner side of the tube wall between the first discharge port and the end of the first feeding tube, a first driving rod is slidably arranged in the circular hole at the other end of the first feeding tube, a first piston and a second piston are axially spaced and arranged at an inner end of the first driving rod, and the first piston is located between the circular hole at the end of the first feeding tube and the second piston The axial thickness of the second piston is smaller than the spacing between the first discharge port and the exposed end of the first feeding pipe, a first feeding port and a first air hole for facilitating the movement of the first piston are provided on the tube wall of the first feeding pipe, the first feeding port is connected to the first feeding pipe through a first valve assembly cooperating with the first piston, the first valve assembly opens to the first after the first piston moves from the first discharge port to the first feeding port and passes through the feeding pipe, and closes to the first feeding pipe after the first piston passes through the first feeding port in the opposite direction, and a first limiting ring for limiting the first piston is provided in the first feeding pipe.

4. The lignin microbial degradation amplification reactor according to claim 3, characterized in that: The first valve assembly includes a cylindrical shell, which is arranged between the first feed port and the first feed pipe and is connected. The axis of the cylindrical shell is perpendicular to the axis of the first feeding pipe. A cylindrical first valve plug is sealed and rotated in the cylindrical shell. A through second feed port is provided on the cylindrical surface of the first valve plug. Two first vortex springs are connected between the two end pins of the first valve plug and the cylindrical shell. A shift block cooperating with the first piston is provided on the cylindrical surface of the first valve plug. A limiting block is provided on the end face of the first valve plug. The limiting block moves in the limiting groove on the inner wall of the cylindrical shell.

5. The amplified lignin degradation reactor of claim 3, characterized in that: All the first feeding pipes on the first feeding pipes are connected to the first liquid pump on the bottom partition of the second frame through the second main pipe arranged on the second frame, and the first driving rods in all the first feeding pipes are synchronously connected through the first connecting frame arranged in the second frame. The first connecting frame is provided with a horizontal rack, and the rack slides in the two first guide sleeves on the second frame. The second frame is provided with a motor, and the output shaft of the motor is provided with a gear meshing with the rack.

6. The amplified lignin microbial degradation reactor according to claim 1, characterized in that: The second adding component includes a cylindrical first silo arranged in a second frame, a third feed port and a second discharge port are provided at one end of the first silo, a second feed pipe and a second discharge pipe for adding lignin particles into the reaction bottle are respectively provided at the third feed port and the second discharge port, a second valve assembly and a third valve assembly are respectively provided in the second feed pipe and the second discharge pipe, the second valve assembly and the third valve assembly have the same structure, the second valve assembly and the third valve assembly are respectively in an open state and a closed state when a negative pressure is generated in the first silo, and are respectively in a closed state and an open state when a positive pressure is generated in the first silo, the second feed pipe is connected to the adsorption port through a fourth main pipe, an outer shell forming a sandwich therewith is provided on the outer side of the first silo, second vent holes connected to the outer shell are evenly and densely distributed on the wall surface of the first silo, an air pipe connected to the inner shell is provided at the end of the outer shell, and the air pipe is connected to the air pump on the second frame through the third main pipe.

7. The amplified lignin microbial degradation reactor according to claim 6, characterized in that: The second valve assembly or the third valve assembly includes a second baffle plate that cooperates with the upper limit strip on the inner wall of the second feed pipe or the second discharge pipe, the second baffle plate is hinged to the support ear on the inner wall of the second feed pipe or the second discharge pipe through a hinge shaft, a second vortex spring is connected between the hinge shaft and the support ear, a sealing gasket that cooperates with the inner wall of the second feed pipe or the second discharge pipe is provided at the swing end of the second baffle plate, and a sealing gasket that cooperates with the second baffle plate is provided on the support ear.

8. The amplified lignin microbial degradation reactor according to claim 1, characterized in that: The third adding component includes a cylindrical second material bin arranged in the second frame, a fourth feed port and a third discharge port are opened at one end of the second material bin, a third feed pipe and a third discharge pipe for filling distilled water into the reaction bottle are respectively arranged at the fourth feed port and the third discharge port, a fourth valve assembly and a fifth valve assembly are respectively arranged in the third feed pipe and the third discharge pipe, the fourth valve assembly has the same structure as the fifth valve assembly, and the fourth valve assembly and the fifth valve assembly are respectively in an open state and a closed state when negative pressure is generated in the second material bin, and are respectively in a closed state and an open state when positive pressure is generated in the second material bin. The third feed pipe is connected to the second liquid pump on the second frame through the fifth main pipe, and the discharge end of the third discharge pipe is provided with a sixth valve assembly, which opens when the fifth valve assembly is opened and closes when the fifth valve assembly is closed. A third piston and two second limiting rings that limit the movement of the third piston are slidably arranged in the second material bin, and a second air hole that facilitates the movement of the third piston is opened on the wall of the second material bin. A second driving rod fixedly connected to a third bolt is slidably arranged in the circular hole at the other end of the second material bin, and the second driving rod is fixedly connected to a second connecting frame in the second frame body that is horizontally driven by an electric push rod.

9. The amplified reactor for lignin microbial degradation according to claim 8, characterized in that: The fourth valve assembly or the fifth valve assembly includes a second guide sleeve, which is fixed in a chamber on the inner wall of a third feed pipe or a third discharge pipe by four circumferentially evenly distributed fixing rods, a first guide rod is slidably arranged in the second guide sleeve, a second valve plug for switching the third feed pipe or the third discharge pipe is arranged at one end of the first guide rod, and a first spring is connected between the second valve plug and the fixing rod to drive the second valve plug to close the third feed pipe or the third discharge pipe.

10. The amplified lignin microbial degradation reactor according to claim 8, characterized in that: The sixth valve assembly includes a spherical shell arranged at the discharge end of the third feed pipe or the third discharge pipe, the wall surface of the spherical shell is provided with a first ring sleeve opposite to the port of the third feed pipe or the third discharge pipe, the first ring sleeve is threadedly connected with a screw sleeve, a cross is arranged in the screw sleeve, a second guide rod is slidably arranged in the circular hole in the middle of the cross, the inner end of the second guide rod is provided with a spherical third valve plug for switching the port of the third feed pipe or the third discharge pipe, and a second spring is connected between the third valve plug and the cross to drive the third valve plug to close the port of the third feed pipe or the third discharge pipe.

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