A biological enzyme preparation device and preparation method for removing odor

Abstract

The invention discloses a biological enzyme preparation device and a preparation method thereof which are beneficial to removing odors, comprising an equipment box, a culture box, a control box and an oxygen supply device. In the invention, a culture dish uses a plurality of air-filled airbag cavities to buffer the flow of culture fluid, and at the same time, a sterile liquid is used to support the flow between the outside of the airbag bag and a shaking seat, which also plays a good supporting role. The flexible state of the culture dish can greatly reduce the mechanical damage to the bacteria during the culture process, and greatly improve the fermentation quality. The culture dish is driven by a motor, a turntable and a first support rod and a second support rod of different heights to perform periodic undulating movements, which can drive the culture fluid to form an ecological water flow state, which is very different from the previous back-and-forth movement mode of a shaking table, which satisfies the activity state of the culture fluid and avoids the generation of a large amount of foam. Less foam can improve the oxygen supply and carbon dioxide discharge efficiency, and further improve the fermentation quality.

Description

Technical Field

[0001] The invention belongs to the technical field of biological enzyme preparations, and relates to a biological enzyme preparation preparation device and a preparation method thereof which are beneficial to removing odors. Background Art

[0002] In recent years, with the improvement of people's living standards and the acceleration of the pace of life, environmental pollution has attracted more and more attention. Following the coal smoke type and photochemical smog type pollution, the third pollution period marked by "indoor air pollution" has begun to affect people's daily life. According to statistics, 90% of human time is spent working, studying and living indoors, and 60% of the time is spent at home. Formaldehyde has become one of the important daily pollutants due to its wide source, high toxicity and long pollution time. The source of indoor formaldehyde comes from indoor decoration. According to a survey by relevant international organizations, 2.8 million people die directly or indirectly from decoration pollution every year in the world, and 30% of the newly built and renovated buildings in the world have toxic gases that are harmful to health. In addition, secondary smoke, sewer odor, garbage odor, etc. in family life have also become major problems affecting people's health. Therefore, how to improve indoor air quality and ensure the health of residents has attracted the attention of relevant departments and the wide attention of the people across the country.

[0003] In order to eliminate formaldehyde and other odor sources, domestic and foreign scholars have adopted a variety of methods to treat them, such as photocatalysis, air catalysis, titanium dioxide-activated carbon fiber synthesis technology and plasma electrocatalysis air purification technology. The above technologies are mainly concentrated on physical and chemical methods. With the advancement of science and technology, biological methods are also attracting more and more attention from researchers. Biocatalyst is a general term for specific microorganisms or their metabolites. It can oxidize and decompose, and utilize corresponding organic compounds or inorganic substances. It has anti-fouling, sterilization and deodorization functions. At present, when preparing biological enzyme preparations that are beneficial to deodorization, the sources of the enzyme preparations are microorganisms, animals and plants, and the main source is microorganisms. Generally, excellent enzyme-producing strains are selected to produce enzymes through fermentation. In order to increase the enzyme concentration in the fermentation broth, in addition to breeding excellent strains and developing genetically engineered bacteria, optimizing the fermentation conditions is also an important improvement direction. In the prior art, a shaker is usually used in combination with a glass culture dish to ferment and produce enzymes. The existing shaker movement mode is easy to produce a large amount of foam on the surface of the culture liquid during fermentation. These foams will cover the surface of the fermentation liquid, affecting the transfer of oxygen and the discharge of carbon dioxide, thereby affecting the fermentation effect. When the speed is lowered, the insufficient movement of the culture liquid will lead to insufficient enzyme concentration. Moreover, the side wall of the glass culture dish will cause mechanical damage to the bacteria when the shaker is in motion, affecting the normal growth and metabolism of the bacteria, and further affecting the yield and quality of the fermentation product. Therefore, it is necessary to design a biological enzyme preparation preparation device and a preparation method thereof that are conducive to removing odors to solve the above problems. Summary of the invention

[0004] The purpose of the present invention is to solve the above problems in the prior art and to provide a biological enzyme preparation device and a preparation method thereof which are beneficial to removing odor.

[0005] To achieve the above-mentioned purpose, the technical scheme of the present invention is: a biological enzyme preparation equipment that is beneficial to the removal of odors, including an equipment box, an incubator, a control box and an oxygen supply device, wherein a control device is arranged inside the control box, a control panel is arranged on the front wall of the control box, the equipment box, the incubator and the control box are arranged in order from bottom to top, a rocking seat is supported on the inner lower wall of the incubator by a rotating support structure, a driving structure for driving the rocking seat to rock is arranged between the equipment box and the incubator, and the upper wall of the rocking seat is supported by a fixed support structure. The fixed support structure is provided with a culture dish, which consists of two groups of concentrically arranged flange rings and air bag bags arranged on the lower walls of the two groups of flange rings. The two groups of flange rings are fixedly connected by two groups of connecting handles. The outer wall of the outer group of the two groups of flange rings is provided with a discharge port. The air bag is annular in top view and U-shaped in front view. The two ends of the U-shaped mouth of the air bag are respectively fixedly connected to the lower walls of the two groups of flange rings. A culture cavity is provided inside the U-shaped air bag, and sterile liquid is provided inside the shaking seat and outside the air bag.

[0006] Preferably, the airbag bag is composed of two layers of inner and outer plastic films, a plurality of airbag cavities are arranged between the two layers of plastic films, and the interiors of the plurality of airbag cavities are all filled with air.

[0007] Preferably, an oxygen supply structure for adding oxygen to the interior of the culture chamber is provided between the incubator and the equipment box, and a temperature control structure for maintaining the temperature inside the incubator is provided inside the incubator.

[0008] Preferably, the driving structure comprises a motor, a turntable, a first support rod and a second support rod, wherein the motor is fixedly connected to the inner upper wall of the equipment box, the motor extension shaft penetrates the upper wall of the equipment box, the lower wall of the incubator and extends into the interior of the incubator, the outer wall of the motor extension shaft and the lower wall of the incubator are sealed by a rotating seal, the turntable is fixedly connected to the end of the motor extension shaft, the first support rod and the second support rod are both fixedly connected to the upper wall of the turntable and are symmetrically distributed with the center of the turntable as the center, the first support rod is longer than the second support rod, and the first support rod and the second support rod are rotatably connected to one end away from the turntable with a group of rollers, and the circumferential outer walls of the two groups of rollers are rollingly connected to the lower wall of the rocking seat.

[0009] Preferably, the rotating support structure includes multiple groups of sliding tubes, which are all fixedly connected to the inner lower wall of the incubator and are equally distributed in a circle with the center of the lower wall of the incubator as the axis. The inner wall of the sliding tube is slidably connected to a sliding rod, and a ball head is fixedly connected to the upper end of the sliding rod. An end of the ball head away from the sliding rod is rotatably connected to a ball joint seat, and an end of the ball joint seat away from the ball head is fixedly connected to the lower wall of the rocking seat, and the ball joint seat is arranged on the lower wall of the rocking seat and close to the circumferential outer wall.

[0010] Preferably, the fixed support structure includes four groups of support rods, and the four groups of support rods are fixedly connected to the circumferential outer wall of the outer group of the two groups of flange rings. The four groups of support rods are equally distributed in a circle with the axis of the flange ring as the center. The end of the support rod away from the flange ring is axially distributed and fixedly connected with two groups of limiting rings in sequence. The upper wall of the rocking seat is provided with four groups of equally distributed suspension grooves, and the four groups of support rods are respectively clamped with the inner side walls of one group of suspension grooves. The support rods and the suspension grooves are limited by two groups of limiting rings, and a buffer pad is provided between the inner side wall of the suspension groove and the outer wall of the support rod.

[0011] Preferably, the oxygen supply structure includes an oxygen tank, an exhaust pump, an oxygen supply pipe and an exhaust pipe, the oxygen supply pipe and the exhaust pipe are fixedly connected to the lower inner wall of the incubator and are respectively close to the left rear and right front of the incubator, the oxygen tank and the exhaust pump are fixedly connected to the lower inner wall of the equipment box, the upper wall of the oxygen tank is fixedly connected with an oxygen supply outlet, the end of the oxygen supply outlet away from the oxygen tank is connected with the oxygen supply pipe through a pipeline and an electric control valve, the right wall of the oxygen tank is fixedly connected with a supplementary inlet, the right wall of the equipment box is fixedly connected with an air intake joint, the supplementary inlet and the air intake joint as well as the air intake joint and the oxygen supply device are connected by pipelines, the upper wall of the exhaust pump is fixedly connected with an exhaust interface, the end of the exhaust interface away from the exhaust pump is connected with the exhaust pipe through a pipeline.

[0012] Preferably, the temperature control structure includes a first electric heater, a second electric heater and a temperature sensor, the first electric heater and the second electric heater are fixedly connected to the lower inner wall of the incubator and are respectively close to the left front side and the right rear side of the incubator, the inner left wall of the incubator is fixedly connected to a bracket, the temperature sensor is fixedly connected to one end of the bracket away from the inner left wall of the incubator, the temperature sensor is a non-contact sensor and the detection end of the temperature sensor is facing the inside of the culture dish.

[0013] Preferably, the bottom of the equipment box is supported by four sets of feet, the front wall of the equipment box is rotatably connected to a maintenance door, the front wall of the incubator is rotatably connected to a closed door, the inner wall of the closed door is fixedly connected to a visual window, and ventilation windows are provided on both left and right side walls of the equipment box.

[0014] According to the above-mentioned method for preparing a biological enzyme preparation device for removing odor, the preparation method comprises the following steps:

[0015] S1. Pour the culture solution of the enzyme-producing strain into the sterilized culture chamber, lift the culture dish into the incubator through the connecting handle, and fix the culture dish and the shaking seat together by inserting the supporting rod into the hanging groove, and start culturing after closing the closed door;

[0016] S2. Set the motor speed, culture time and culture temperature through the control panel, start the motor, and the motor drives the turntable to rotate. When the turntable rotates, the first support rod and the second support rod with different heights are used to periodically adjust the inclination angle of the rocking seat, so that the rocking seat drives the culture dish to form a periodic undulating motion, thereby driving the culture liquid in the culture chamber to continuously flow along the annular inner wall of the culture chamber. The flow speed of the culture liquid can be adjusted by adjusting the motor speed. During the flow process, the sterile liquid on the airbag bag and the outer wall of the airbag bag is used for buffering to avoid mechanical damage to the bacteria;

[0017] S3. During the flow process, oxygen is continuously supplied to the culture solution through an oxygen tank and an oxygen supply tube to ensure that the enzyme-producing strains are fully exposed to oxygen. At the same time, the carbon dioxide generated during the culture process inside the incubator is sucked away through an air pump and an air suction tube to maintain the air quality inside the incubator.

[0018] S4, continuously detecting the temperature of the culture solution through a temperature sensor, and when the temperature is lower than a set temperature, heating the culture solution through the first electric heater and the second electric heater, and when the temperature reaches the set temperature, the first electric heater and the second electric heater are turned off;

[0019] S5. After the cultivation is completed, the closed door is opened, and the culture dish is lifted out of the rocking seat through two sets of connecting handles, and is taken out of the incubator, and the culture dish is tilted toward one side of the discharge port, and the culture solution is poured out through the discharge port, and then the biological enzyme liquid is obtained after separation and extraction, and the biological enzyme liquid is added with a protective agent, humic acid, a preservative and a plant essence and mixed evenly to obtain a biological enzyme preparation that is beneficial to removing odors.

[0020] By adopting the above technical solution, the beneficial effects of the present invention are:

[0021] 1. In the present invention, the culture dish uses multiple air-filled air bag cavities to buffer the flow of the culture solution, and at the same time, the sterile liquid is used to support the outside of the air bag and the shaking seat, which also plays a good supporting role. The flexible state of the culture dish can greatly reduce the mechanical damage to the bacteria during the culture process, greatly improving the fermentation quality.

[0022] 2. In the present invention, the culture dish is driven by a motor, a turntable, and a first support rod and a second support rod of different heights to perform periodic undulating motions, which can drive the culture solution to form an ecological water flow state, which is very different from the back-and-forth motion of the shaking table in the past. It can satisfy the activity state of the culture solution and avoid the generation of a large amount of foam. Less foam can improve the oxygen supply and carbon dioxide discharge efficiency, further improving the fermentation quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0024] Figure 2 It is a partial cross-sectional view of the internal structure of the equipment box of the present invention.

[0025] Figure 3 It is a partial cross-sectional view of the internal structure of the incubator of the present invention.

[0026] Figure 4 The present invention Figure 3 A partial enlarged view of point A in the middle.

[0027] Figure 5 It is a partial schematic diagram of the connection structure of the bracket and the temperature sensor of the present invention.

[0028] Figure 6 It is a partial cross-sectional view of the flange ring and air bag connection structure of the present invention.

[0029] Figure 7 It is a partial cross-sectional view of the rocking seat, sliding rod, sliding tube, ball joint seat and ball head connection structure of the present invention.

[0030] Figure 8 It is a partial cross-sectional view of the incubator, the equipment box, the motor, the turntable, the first support rod, the second support rod and the rocking seat connection structure of the present invention.

[0031] In the figure, 1, equipment box; 2, incubator; 3, control box; 4, foot; 5, maintenance door; 6, closed door; 7, visual window; 8, control panel; 9, ventilation window; 10, air inlet connector; 11, oxygen tank; 12, oxygen supply outlet; 13, supplementary inlet; 14, vacuum pump; 15, vacuum interface; 16, air bag; 17, air bag cavity; 18, incubator cavity; 19, rocking seat; 1901, hanging slot; 20, flange ring; 2 001, discharge port; 21, connecting handle; 22, first electric heater; 23, second electric heater; 24, oxygen supply pipe; 25, exhaust pipe; 26, bracket; 27, support rod; 28, limit ring; 29, buffer pad; 30, temperature sensor; 31, sliding tube; 32, sliding rod; 33, ball head; 34, ball joint seat; 35, motor; 36, turntable; 37, first support rod; 38, second support rod; 39, roller. DETAILED DESCRIPTION

[0032] In order to further explain the technical solution of the present invention, the present invention is described in detail below through specific embodiments.

[0033] like Figures 1 to 8 As shown, this embodiment proposes a biological enzyme preparation device that is beneficial to removing odors, including an equipment box 1, an incubator 2, a control box 3 and an oxygen supply device, a control device is arranged inside the control box 3, a control panel 8 is arranged on the front wall of the control box 3, the equipment box 1, the incubator 2 and the control box 3 are arranged in order from bottom to top, the bottom of the equipment box 1 is supported by four groups of feet 4, a maintenance door 5 is rotatably connected to the front wall of the equipment box 1, a closed door 6 is rotatably connected to the front wall of the incubator 2, a visual window 7 is fixedly connected to the inner wall of the closed door 6, and ventilation windows 9 are arranged on the left and right side walls of the equipment box 1. The control device is a mature technology currently available on the market, and is used to control the entire culture process according to set parameters;

[0034] In order to facilitate the driving structure to drive the shaking seat 19 to move, the inner lower wall of the incubator 2 is supported by the shaking seat 19 through the rotating support structure. The rotating support structure includes multiple groups of sliding tubes 31. The multiple groups of sliding tubes 31 are fixedly connected to the inner lower wall of the incubator 2 and are equally distributed in a circle with the center of the lower wall of the incubator 2 as the axis. The inner wall of the sliding tube 31 is slidably connected to a sliding rod 32. The upper end of the sliding rod 32 is fixedly connected to a ball head 33. The end of the ball head 33 away from the sliding rod 32 is rotatably connected to a ball joint seat 34. The end of the ball joint seat 34 away from the ball head 33 is fixedly connected to the lower wall of the shaking seat 19. The ball joint seat 34 is arranged on the lower wall of the shaking seat 19 and close to the outer wall of the circle. When the driving structure moves, the axial position of the shaking seat 19 is limited by the rotating support structure. The horizontal level at the center of the shaking seat 19 does not change, and the area near the edge follows the action of the driving structure to perform periodic ups and downs.

[0035] In order to realize the ecological flow of the culture solution, a driving structure for driving the rocking seat 19 to rock is arranged between the equipment box 1 and the incubator 2, including a driving structure motor 35, a turntable 36, a first support rod 37 and a second support rod 38. The motor 35 is fixedly connected to the inner upper wall of the equipment box 1, and the motor 35 extends through the upper wall of the equipment box 1 and the lower wall of the incubator 2 and extends into the interior of the incubator 2. The outer wall of the motor 35 extension shaft is sealed with the lower wall of the incubator 2 by a rotating seal. The turntable 36 is fixedly connected to the end of the motor 35 extension shaft. The first support rod 37 and the second support rod 38 are both fixedly connected to the upper wall of the turntable 36 and are symmetrically distributed with the center of the turntable 36 as the center. The length of the first support rod 37 is greater than that of the second support rod 38. The ends of the first support rod 37 and the second support rod 38 away from the turntable 36 are respectively rotatably connected with a group of rollers 39. The outer circumferential walls of the two groups of rollers 39 are both connected to the lower wall of the rocking seat 19 in a rolling manner. After the motor 35 is started, the turntable 36 is driven to rotate. When the turntable 36 rotates, the first support rod 37 and the second support rod 38 are driven to rotate. The length of the first support rod 37 is greater than the second support rod 38, so that when the rocking seat 19 contacts the first support rod 37 and the second support rod 38 through the rollers 39, the side close to the second support rod 38 is lower than the side close to the first support rod 37. When the turntable 36 rotates and drives the first support rod 37 and the second support rod 38 to rotate, the circumferential side of the rocking seat 19 forms a continuous undulating motion, thereby driving the culture solution in the culture chamber 18 to form a flowing state. The culture solution in the flowing state can improve the contact efficiency with oxygen and the carbon dioxide discharge efficiency. In addition, the culture solution has less collision with the side wall of the culture chamber 18, so the bacteria are not easily damaged and foam is not easily generated.

[0036] In order to facilitate the fixation and removal of the culture dish, the upper wall of the rocking seat 19 is provided with a culture dish through a fixed support structure, and the fixed support structure includes four groups of support rods 27, and the four groups of support rods 27 are fixedly connected to the circumferential outer wall of the outer group of the two groups of flange rings 20, and the four groups of support rods 27 are equally distributed in the circumference with the axis of the flange ring 20 as the center, and the end of the support rod 27 away from the flange ring 20 is axially distributed and fixedly connected with two groups of limit rings 28 in sequence, and the upper wall of the rocking seat 19 is provided with four groups of equally distributed hanging grooves 1901, and the four groups of support rods 27 are respectively connected to a group of hanging grooves 190 1, the support rod 27 and the hanging groove 1901 are limited by two sets of limiting rings 28, and a buffer pad 29 is provided between the inner wall of the hanging groove 1901 and the outer wall of the support rod 27. The culture dish can be taken by holding the connecting handle 21, and the culture dish can be fixed after the four sets of support rods 27 are connected with the four sets of hanging grooves 1901, so that the culture dish will not be relatively displaced with the shaking seat 19 when the shaking seat 19 moves. The buffer pad 29 is provided for buffering and is also used to clamp the support rod 27 through the elasticity of the buffer pad 29 so that it will not fall out of the hanging groove 1901 by itself;

[0037] In order to achieve a flexible culture state, the culture dish is composed of two sets of concentrically arranged flange rings 20 and an air bag 16 arranged on the lower wall of the two sets of flange rings 20. The two sets of flange rings 20 are fixedly connected by two sets of connecting handles 21. The outer wall of the outer set of the two sets of flange rings 20 is provided with a discharge port 2001. The air bag 16 is annular in top view and U-shaped in front view. The two ends of the U-shaped mouth of the air bag 16 are respectively fixedly connected to the lower wall of the two sets of flange rings 20. The air bag 16 is provided with a culture cavity 18 inside the U-shaped air bag. The shaking seat A sterile liquid is arranged inside 19 and outside the air bag 16. The air bag 16 is composed of two layers of plastic films, inner and outer. A plurality of air bag cavities 17 are arranged between the two layers of plastic films. The interiors of the plurality of air bag cavities 17 are all filled with air. Through the plurality of air bag cavities 17 filled with air, the culture liquid will not have a hard collision when contacting the side wall of the culture cavity 18, thereby playing a good buffering role. At the same time, a sterile liquid is arranged between the outer wall of the air bag 16 and the inner wall of the shaking seat 19, thereby playing the same buffering and protective role, thereby effectively improving the fermentation quality.

[0038] In order to ensure sufficient oxygen supply during fermentation, an oxygen supply structure for adding oxygen to the culture chamber 18 is provided between the incubator 2 and the equipment box 1. The oxygen supply structure includes an oxygen tank 11, an air pump 14, an oxygen supply pipe 24 and an air pump 25. The oxygen supply pipe 24 and the air pump 25 are both fixedly connected to the lower wall of the inner side of the incubator 2 and are respectively close to the left rear and right front of the incubator 2. The oxygen tank 11 and the air pump 14 are both fixedly connected to the lower wall of the inner side of the equipment box 1. The upper wall of the oxygen tank 11 is fixedly connected with an oxygen supply outlet 12. The end of the oxygen supply outlet 12 away from the oxygen tank 11 is connected to the oxygen supply pipe 24 through a pipeline and an electric control valve. The right wall of the oxygen tank 11 is fixedly connected with a supplementary inlet 1 3. An air inlet connector 10 is fixedly connected to the right wall of the equipment box 1. The supplementary inlet 13 and the air inlet connector 10 as well as the air inlet connector 10 and the oxygen supply device are connected via pipelines. An air extraction interface 15 is fixedly connected to the upper wall of the air extraction pump 14. The end of the air extraction interface 15 away from the air extraction pump 14 is connected to the air extraction pipe 25 via a pipeline. The oxygen tank 11 continuously supplies oxygen to the liquid surface of the culture solution via the oxygen supply outlet 12, the pipeline and the oxygen supply pipe 24. The culture solution is continuously supplemented with oxygen when it flows, which effectively improves the contact efficiency with oxygen and the fermentation efficiency. At the same time, when the carbon dioxide generated during the fermentation process is deposited at the lower part of the incubator 2, it is extracted by the air extraction pump 14 through the air extraction pipe 25.

[0039] In order to control the temperature inside the incubator 2, a temperature control structure for maintaining the temperature inside the incubator 2 is provided inside the incubator 2, and the temperature control structure includes a first electric heater 22, a second electric heater 23 and a temperature sensor 30. The first electric heater 22 and the second electric heater 23 are both fixedly connected to the lower inner wall of the incubator 2 and are respectively close to the left front side and the right rear side of the incubator 2. A bracket 26 is fixedly connected to the inner left wall of the incubator 2. The temperature sensor 30 is fixedly connected to one end of the bracket 26 away from the inner left wall of the incubator 2. The temperature sensor 30 is a non-contact sensor and the detection end of the temperature sensor 30 faces the inside of the culture dish. The temperature of the culture liquid surface is continuously detected by the temperature sensor 30 in a non-contact manner. When the temperature is lower than the set temperature, the first electric heater 22 and the second electric heater 23 are started by the control device to heat the air inside the incubator 2. When the temperature reaches the set temperature, the first electric heater 22 and the second electric heater 23 are turned off.

[0040] According to the above-mentioned method for preparing a biological enzyme preparation device for removing odor, the preparation method comprises the following steps:

[0041] S1. Pour the culture solution containing the enzyme-producing strain into the sterilized culture chamber 18, lift the culture dish into the incubator 2 by connecting the handle 21, and fix the culture dish and the rocking seat 19 together by inserting the support rod 27 into the hanging groove 1901, and start culturing after closing the closed door 6;

[0042] S2, set the speed of the motor 35, the culture time and the culture temperature through the control panel 8, start the motor 35, and the motor 35 drives the turntable 36 to rotate. When the turntable 36 rotates, the first support rod 37 and the second support rod 38 of different heights periodically adjust the inclination angle of the rocking seat 19, so that the rocking seat 19 drives the culture dish to form a periodic undulating motion, thereby driving the culture liquid in the culture chamber 18 to continuously flow along the annular inner wall of the culture chamber 18. The flow speed of the culture liquid can be adjusted by adjusting the speed of the motor 35. During the flow process, the sterile liquid of the airbag bag 16 and the outer wall of the airbag bag 16 is used for buffering to avoid mechanical damage to the bacteria;

[0043] S3, during the flow process, oxygen is continuously supplied to the culture solution through the oxygen tank 11 and the oxygen supply pipe 24 to ensure that the enzyme-producing strains are fully exposed to oxygen, and at the same time, the carbon dioxide generated during the culture process inside the incubator 2 is sucked away by the suction pump 14 and the suction pipe 25 to maintain the air quality inside the incubator 2;

[0044] S4, continuously detecting the temperature of the culture solution through the temperature sensor 30, when it is lower than the set temperature, heating it through the first electric heater 22 and the second electric heater 23, and when the temperature reaches the set temperature, the first electric heater 22 and the second electric heater 23 are turned off;

[0045] S5. After the cultivation is completed, the closed door 6 is opened, and the culture dish is lifted out of the rocking seat 19 through the two sets of connecting handles 21, and taken out of the incubator 2, and the culture dish is tilted toward the discharge port 2001, and the culture solution is poured out through the discharge port 2001, and then the biological enzyme liquid is obtained after separation and extraction, and the biological enzyme liquid is added with a protective agent, humic acid, a preservative and a plant essence and mixed evenly to obtain a biological enzyme preparation that is beneficial to removing odors.

[0046] Working principle: When the driving structure is in motion, the axial position of the rocking seat 19 is limited by the rotating support structure, the center of the rocking seat 19 does not change horizontally, and the edge thereof follows the motion of the driving structure to perform periodic undulating motions; when the turntable 36 rotates, driving the first support rod 37 and the second support rod 38 to rotate, the peripheral side of the rocking seat 19 forms a continuous undulating motion, thereby driving the culture fluid inside the culture chamber 18 to form a flowing state, and the culture fluid in the flowing state can improve the contact efficiency with oxygen and improve the carbon dioxide discharge efficiency, and the culture fluid and the culture chamber 18 are in a stable state. There is less impact on the side wall, the bacteria are not easily damaged, and it is not easy to generate foam; the culture dish can be taken by holding the connecting handle 21, and the culture dish can be fixed by the four groups of supporting rods 27 and the four groups of hanging grooves 1901, so that the culture dish will not be relatively displaced with the shaking seat 19 when the shaking seat 19 moves. The buffer pad 29 is provided for buffering and is also used to clamp the supporting rod 27 through the elasticity of the buffer pad 29 so that it will not fall out of the hanging groove 1901 by itself; the airbag bag 16 is composed of two layers of inner and outer plastic films, and a plurality of airbag cavities 17 are provided between the two layers of plastic films. The interior of the multiple airbag cavities 17 is filled with air. Through the multiple airbag cavities 17 filled with air, the culture liquid will not have a hard collision when it contacts the side wall of the culture chamber 18, which plays a good buffering role. At the same time, a sterile liquid is arranged between the outer wall of the airbag bag 16 and the inner wall of the shaking seat 19, which also plays the same buffering and protective role, effectively improving the fermentation quality; the oxygen tank 11 continuously supplies oxygen to the liquid surface of the culture liquid through the oxygen supply outlet 12, the pipeline and the oxygen supply pipe 24. The culture liquid is continuously supplemented with oxygen when it flows, which effectively improves the contact efficiency with oxygen and improves the fermentation efficiency. rate, and the carbon dioxide generated during the fermentation process is deposited at the bottom of the incubator 2 and is extracted by the vacuum pump 14 through the vacuum pipe 25; the temperature sensor 30 is a non-contact sensor and the detection end of the temperature sensor 30 is facing the inside of the culture dish. The temperature of the culture liquid surface is continuously detected by the temperature sensor 30 in a non-contact manner. When it is lower than the set temperature, the first electric heater 22 and the second electric heater 23 are started by the control device to heat the air inside the incubator 2. When the temperature reaches the set temperature, the first electric heater 22 and the second electric heater 23 are turned off.

[0047] The above components are all common standard parts or components known to those skilled in the art, and their structures and principles can be known to those skilled in the art through technical manuals or conventional experimental methods.

[0048] The above embodiments and drawings do not limit the product form and style of the present invention. Any appropriate changes or modifications made by ordinary technicians in the relevant technical field should be deemed to be within the patent scope of the present invention.

Claims

1. A biological enzyme preparation device for removing odor, characterized in that: The invention comprises an equipment box (1), an incubator (2), a control box (3) and an oxygen supply device, wherein a control device is arranged inside the control box (3), a control panel (8) is arranged on the front wall of the control box (3), the equipment box (1), the incubator (2) and the control box (3) are arranged in order from bottom to top, a rocking seat (19) is supported on the inner lower wall of the incubator (2) via a rotating support structure, a driving structure for driving the rocking seat (19) to rock is arranged between the equipment box (1) and the incubator (2), a culture dish is arranged on the upper wall of the rocking seat (19) via a fixed support structure, and the culture dish has two groups of concentrically arranged The invention is composed of a flange ring (20) and an air bag (16) arranged on the lower wall of the two groups of flange rings (20), the two groups of flange rings (20) are fixedly connected by two groups of connecting handles (21), the outer wall of the outer group of the two groups of flange rings (20) is provided with a discharge port (2001), the air bag (16) is annular in top view and U-shaped in front view, the two ends of the U-shaped mouth of the air bag (16) are respectively fixedly connected to the lower wall of the two groups of flange rings (20), a culture chamber (18) is arranged inside the U-shaped air bag (16), and a sterile liquid is arranged inside the shaking seat (19) and outside the air bag (16).

2. The bio-enzyme preparation device for removing odor according to claim 1, characterized in that: The airbag bag (16) is composed of two layers of plastic films, an inner layer and an outer layer. A plurality of airbag cavities (17) are arranged between the two layers of plastic films. The interiors of the plurality of airbag cavities (17) are all filled with air.

3. The bio-enzyme preparation device for removing odor according to claim 2, characterized in that: An oxygen supply structure for adding oxygen to the interior of the culture chamber (18) is provided between the culture box (2) and the equipment box (1), and a temperature control structure for maintaining the temperature inside the culture box (2) is provided inside the culture box (2).

4. The device for preparing a biological enzyme preparation for removing odor according to claim 3, characterized in that: The driving structure comprises a motor (35), a rotating disk (36), a first support rod (37) and a second support rod (38); the motor (35) is fixedly connected to the upper wall of the inner side of the equipment box (1); the motor (35) extends through the upper wall of the equipment box (1) and the lower wall of the incubator (2) and extends into the interior of the incubator (2); the outer wall of the motor (35) extension shaft is sealed with the lower wall of the incubator (2) by a rotating seal; the rotating disk (36) is fixedly connected to the motor (35) extension shaft The first support rod (37) and the second support rod (38) are both fixedly connected to the upper wall of the turntable (36) and are symmetrically distributed with the center of the turntable (36) as the center. The length of the first support rod (37) is greater than that of the second support rod (38). One end of the first support rod (37) and the second support rod (38) away from the turntable (36) is rotatably connected to a group of rollers (39), and the outer walls of the circumferences of the two groups of rollers (39) are rollingly connected to the lower wall of the rocking seat (19).

5. The device for preparing a biological enzyme preparation for removing odor according to claim 4, characterized in that: The rotation support structure comprises a plurality of groups of sliding tubes (31), the plurality of groups of sliding tubes (31) are all fixedly connected to the inner lower wall of the incubator (2) and are equally distributed in a circle with the center of the lower wall of the incubator (2) as the axis, the inner wall of the sliding tube (31) is slidably connected to a sliding rod (32), the upper end of the sliding rod (32) is fixedly connected to a ball head (33), the end of the ball head (33) away from the sliding rod (32) is rotatably connected to a ball joint seat (34), the end of the ball joint seat (34) away from the ball head (33) is fixedly connected to the lower wall of the shaking seat (19), and the ball joint seat (34) is arranged on the lower wall of the shaking seat (19) and close to the outer wall of the circle.

6. The device for preparing a biological enzyme preparation for removing odor according to claim 5, characterized in that: The fixed support structure comprises four groups of support rods (27), the four groups of support rods (27) are all fixedly connected to the circumferential outer wall of the outer group of the two groups of flange rings (20), the four groups of support rods (27) are all equally distributed in a circle with the axis of the flange ring (20) as the center, the end of the support rod (27) away from the flange ring (20) is axially distributed and fixedly connected with two groups of limiting rings (28) in sequence, the upper wall of the rocking seat (19) is provided with four groups of equally distributed hanging grooves (1901), the four groups of support rods (27) are respectively clamped with the inner wall of one group of hanging grooves (1901), the support rods (27) and the hanging grooves (1901) are limited by the two groups of limiting rings (28), and a buffer pad (29) is provided between the inner wall of the hanging groove (1901) and the outer wall of the support rod (27).

7. The device for preparing a biological enzyme preparation for removing odor according to claim 6, characterized in that: The oxygen supply structure comprises an oxygen tank (11), an air pump (14), an oxygen supply pipe (24) and an air pump (25); the oxygen supply pipe (24) and the air pump (25) are both fixedly connected to the lower wall of the inner side of the incubator (2) and are respectively close to the left rear and right front of the incubator (2); the oxygen tank (11) and the air pump (14) are both fixedly connected to the lower wall of the inner side of the equipment box (1); the upper wall of the oxygen tank (11) is fixedly connected with an oxygen supply outlet (12); the oxygen supply outlet (12) is far from one end of the oxygen tank (11). The oxygen tank (11) is connected to the oxygen supply pipe (24) through a pipeline and an electric control valve. The right wall of the oxygen tank (11) is fixedly connected to a supplementary inlet (13). The right wall of the equipment box (1) is fixedly connected to an air intake connector (10). The supplementary inlet (13) and the air intake connector (10) as well as the air intake connector (10) and the oxygen supply device are connected through pipelines. The upper wall of the air pump (14) is fixedly connected to an air extraction interface (15). The end of the air extraction interface (15) away from the air extraction pump (14) is connected to the air extraction pipe (25) through a pipeline.

8. The device for preparing a biological enzyme preparation for removing odor according to claim 7, characterized in that: The temperature control structure comprises a first electric heater (22), a second electric heater (23) and a temperature sensor (30); the first electric heater (22) and the second electric heater (23) are both fixedly connected to the lower inner wall of the incubator (2) and are respectively close to the left front side and the right rear side of the incubator (2); the inner left wall of the incubator (2) is fixedly connected to a bracket (26); the temperature sensor (30) is fixedly connected to an end of the bracket (26) away from the inner left wall of the incubator (2); the temperature sensor (30) is a non-contact sensor and the detection end of the temperature sensor (30) faces the interior of the culture dish.

9. The device for preparing a biological enzyme preparation for removing odor according to claim 8, characterized in that: The bottom of the equipment box (1) is supported by four groups of feet (4); the front wall of the equipment box (1) is rotatably connected to a maintenance door (5); the front wall of the incubator (2) is rotatably connected to a closed door (6); the inner wall of the closed door (6) is fixedly connected to a visual window (7); and the left and right side walls of the equipment box (1) are both provided with ventilation windows (9).

10. The method for preparing a biological enzyme preparation device for removing odor according to claim 9, characterized in that: The preparation method comprises the following steps: S1. Pour the culture solution containing the enzyme-producing strain into the sterilized culture chamber (18), lift the culture dish into the culture box (2) through the connecting handle (21), and fix the culture dish and the rocking seat (19) together by inserting the supporting rod 27 into the hanging groove (1901), and start culturing after closing the closed door (6); S2, setting the speed of the motor (35), the culture time and the culture temperature through the control panel (8), starting the motor (35), and the motor (35) driving the turntable (36) to rotate. When the turntable (36) rotates, the first support rod (37) and the second support rod (38) of different heights periodically adjust the inclination angle of the rocking seat (19), so that the rocking seat (19) drives the culture dish to form a periodic undulating motion, thereby driving the culture liquid in the culture chamber (18) to continuously flow along the annular inner wall of the culture chamber (18). The flow speed of the culture liquid can be adjusted by adjusting the speed of the motor (35). During the flow process, the sterile liquid of the air bag (16) and the outer wall of the air bag (16) is used for buffering to avoid mechanical damage to the bacteria; S3, during the flow process, oxygen is continuously supplied to the culture solution through the oxygen tank (11) and the oxygen supply pipe (24) to ensure that the enzyme-producing strain is fully exposed to oxygen, and at the same time, the carbon dioxide generated during the culture process inside the incubator (2) is sucked away through the suction pump (14) and the suction pipe (25) to maintain the air quality inside the incubator (2); S4, continuously detecting the temperature of the culture solution through the temperature sensor (30), and when the temperature is lower than the set temperature, raising the temperature through the first electric heater (22) and the second electric heater (23), and turning off the first electric heater (22) and the second electric heater (23) when the temperature reaches the set temperature; S5. After the cultivation is completed, the closed door (6) is opened, and the culture dish is lifted out of the rocking seat (19) through the two sets of connecting handles (21), and taken out of the incubator (2), and the culture dish is tilted toward the discharge port (2001), and the culture liquid is poured out through the discharge port (2001), and then separated and extracted to obtain a biological enzyme liquid, and the biological enzyme liquid is added with a protective agent, humic acid, a preservative and a plant essence and mixed evenly to obtain a biological enzyme preparation that is beneficial to removing odors.

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