Dynamic 3D suspension culture device for embryos

Abstract

The invention discloses an embryo dynamic 3D suspension culture device which comprises a base, a plurality of placing grooves are uniformly formed in the upper end of the base, culture plates are placed in the placing grooves, a plurality of culture grooves are uniformly formed in the culture plates, stirring assemblies used in cooperation with the culture plates are arranged in the placing grooves, and sealing plates are arranged at the upper ends of the culture grooves. A water inlet pipeline, a water outlet pipeline, an air inlet pipeline and an air outlet pipeline are arranged on the sealing plate, and a water inlet assembly, a water outlet assembly, an air inlet assembly and an air outlet assembly are arranged in the base. A culture solution is uniformly stirred through the stirring assembly, uniform suspension of cells or organoid in the culture solution is promoted, cell precipitation is prevented, blood flow or body fluid flow in a living body is simulated by entering and exiting the culture solution at a constant speed and controlling shearing force generated by liquid flow, and therefore a dynamic 3D environment is provided; and culture conditions closer to reality are provided.

Description

Technical Field

[0001] The invention relates to the technical field of biological experimental equipment, and in particular to a dynamic 3D embryo suspension culture device. Background Art

[0002] With the development of in vitro culture technologies such as embryos, organoids, and blastocysts, three-dimensional suspension culture has become an important means of studying cell and organ models. It allows cells to grow in three-dimensional space without contacting solid surfaces. This culture method is particularly suitable for cells that grow in suspension in their natural state. Most of the existing culture systems focus on planar two-dimensional (2D) culture or static three-dimensional (3D) culture. However, these traditional methods have the following problems: 1) Lack of dynamic culture environment: Existing suspension culture devices mostly rely on static culture and lack the dynamic environment in vivo, such as shear force and fluid flow. 2) Poor culture uniformity: Under static culture, cells are unevenly distributed in the culture medium, affecting the development and differentiation of embryos or organoids. 3) Difficult to simulate real physiological conditions: Traditional culture devices cannot effectively simulate the complex fluid dynamics and environmental changes in the body. Therefore, there is an urgent need for a new dynamic 3D suspension culture device that can provide continuous fluid flow, shear force and gas exchange, simulate the in vivo environment, and promote the development and maturation of biological models such as embryos, organoids or blastocysts. Summary of the invention

[0003] The purpose of the present invention is to provide an embryo dynamic 3D suspension culture device. During the culture process, a constant rotation is provided by a stirring component, and then the inflow and outflow of the culture fluid are controlled to ensure uniform flow and suspension of the liquid, thereby forming a microscopic shear force.

[0004] The above technical objectives of the present invention are achieved through the following technical solutions:

[0005] A dynamic 3D suspended culture device for embryos comprises a base, wherein a plurality of placement grooves are evenly arranged at the upper end of the base, culture plates are placed in the placement grooves, a plurality of culture grooves are evenly arranged on the culture plates, a stirring assembly used in conjunction with the culture plates is arranged in the placement grooves, a sealing plate is arranged at the upper end of the culture grooves, a water inlet pipeline, a water outlet pipeline, an air inlet pipeline and an air outlet pipeline are arranged on the sealing plate, a water inlet assembly, a water outlet assembly, an air inlet assembly and an air outlet assembly are arranged in the base, the water inlet assembly is connected to the culture groove through the water inlet pipeline, the water outlet assembly is connected to the culture groove through the water outlet pipeline, the air inlet assembly is connected to the culture groove through the air inlet pipeline, and the air outlet assembly is connected to the culture groove through the air outlet pipeline.

[0006] By adopting the above technical solution, the stirring component can drive the culture fluid in the culture tank to rotate at a uniform speed, allowing the cells to be evenly suspended in the culture fluid to prevent cell precipitation, while the water inlet pipe, water outlet pipe, water inlet component and water outlet component can make the culture fluid flow evenly, which not only simulates the blood flow or body fluid flow in the organism, but also replaces the culture fluid, both of which are conducive to the formation of microscopic shear force. The air inlet pipe, air outlet pipe, air inlet component and air outlet component can control the gas concentration in the culture environment to maintain an optimal level to support the metabolic needs of cells or embryos.

[0007] The present invention is further configured as follows: the stirring assembly includes a stirring bar and a magnetic stirrer, the magnetic stirrer is provided with multiple and evenly installed at the bottom of the culture tank, the magnetic stirrers are installed one by one at the bottom of the culture tank, and the stirring bar is placed in the culture tank.

[0008] By adopting the above technical solution, the magnetic stirrer drives the stirrer to rotate through magnetic force, thereby stirring the culture solution in the culture tank, which is convenient for repeated use and easy to clean.

[0009] The present invention is further configured as follows: the water inlet pipeline includes a first insertion tube, a first filter screen, a water inlet main pipe and a water inlet branch; the sealing plate is a hollow structure; a plurality of first insertion tubes are provided and are vertically inserted one by one in the culture tank; the first filter screen is installed at the lower end of the first insertion tube; the upper end of the first insertion tube is detachably connected to the water inlet branch; the water inlet branch is arranged in the sealing plate; the other end of the water inlet branch is connected to the water inlet main pipe; the other end of the water inlet main pipe passes through the sealing plate and is detachably connected to the water inlet assembly;

[0010] The water outlet pipeline includes a second insertion tube, a second filter, a water outlet main pipe and a water outlet branch. The second insertion tube is provided in plurality and is vertically inserted one by one in the culture tank. The first insertion tube and the second insertion tube are symmetrically installed in the culture tank. The second filter is installed at the lower end of the second insertion tube. The upper end of the second insertion tube is detachably connected to the water outlet branch. The water outlet branch is provided in the sealing plate. The other end of the water outlet branch is connected to the water outlet main pipe. The other end of the water outlet main pipe passes through the sealing plate and is detachably connected to the water outlet component.

[0011] By adopting the above technical solution, the first insertion tube and the second insertion tube are both inserted into the culture tank from the upper end, which facilitates the later replacement of the first insertion tube and the second insertion tube and the culture plate without affecting the reuse of other pipelines.

[0012] The present invention is further configured as follows: the filter aperture sizes of the first filter screen and the second filter screen are both 3-8 μm.

[0013] By adopting the above technical solution, the first filter screen is used to prevent cells from entering the first insertion tube, and the second filter screen is used to filter the culture solution.

[0014] The present invention is further configured as follows: the water inlet assembly comprises a first peristaltic pump and a culture solution storage tank, the first peristaltic pump and the culture solution storage tank are both arranged in the base, and the water inlet main pipe is connected to the culture solution storage tank after passing through the first peristaltic pump;

[0015] The water outlet assembly includes a second peristaltic pump and a waste liquid collection tank, both of which are installed in the base, and the water outlet main pipe is connected to the waste liquid collection tank after passing through the second peristaltic pump.

[0016] By adopting the above technical solution, the water inlet component sucks the culture solution in the culture solution storage tank into the culture tank through the first peristaltic pump, and the water outlet component discharges the culture solution in the culture tank into the waste liquid collection tank through the second peristaltic pump.

[0017] The present invention is further configured as follows: the air intake pipeline includes an air intake pipe and a gas filter, one end of the air intake pipe is connected to the culture tank, and the other end of the air intake pipe is connected to the air intake assembly through the gas filter;

[0018] The air outlet pipeline includes an air outlet pipe, one end of which is connected to the culture tank, and the other end of which is connected to the air outlet component.

[0019] By adopting the above technical solution, both the air inlet pipeline and the air outlet pipeline are simple air pipes with a simple and convenient structure.

[0020] The present invention is further configured as follows: the air intake assembly comprises a solenoid valve, a nitrogen storage tank and a carbon dioxide storage tank, the solenoid valve, the nitrogen storage tank and the carbon dioxide storage tank are all arranged in the base, two air intake pipes are arranged, one end of the two air intake pipes away from the sealing plate is respectively connected to the nitrogen storage tank and the carbon dioxide storage tank, and the solenoid valve is installed on the air intake pipe;

[0021] The air outlet component comprises a third peristaltic pump, the third peristaltic pump is arranged in the base, and the air outlet pipe is arranged on the third peristaltic pump.

[0022] By adopting the above technical solution, the air intake assembly uses the pressure of the nitrogen storage tank and the carbon dioxide storage tank to open the solenoid valve, and the nitrogen and carbon dioxide enter the culture tank by themselves. When the gas is discharged, it needs to be exhausted by an air pump to ensure the airtightness of the culture tank.

[0023] The present invention is further configured as follows: an air pressure sensor, an oxygen concentration sensor, a carbon dioxide concentration sensor and a temperature sensor are installed at the lower end of the sealing plate, a control panel is provided on the base, and the magnetic stirrer, the first peristaltic pump, the second peristaltic pump, the third peristaltic pump, the solenoid valve, the air pump, the air pressure sensor, the oxygen concentration sensor, the carbon dioxide concentration sensor and the temperature sensor are all electrically connected to the control panel.

[0024] By adopting the above technical solution, the air pressure sensor detects the air pressure in the culture tank, the oxygen concentration sensor detects the oxygen concentration in the culture tank, the carbon dioxide concentration sensor detects the carbon dioxide concentration in the culture tank, and the temperature sensor detects the temperature in the culture tank, so as to better control the cell culture.

[0025] The present invention is further configured as follows: a heating element and a weighing sensor are arranged at the bottom of the placement groove, the heating element and the weighing sensor are electrically connected to the control panel, an openable cover is arranged at the upper end of the base, a sterilization lamp is arranged in the cover, and the sterilization lamp is electrically connected to the control panel.

[0026] By adopting the above technical solution, it is convenient to heat and weigh the culture plate. Heating is to simulate the in vivo environment, while weighing is to detect the changes in the culture solution in real time to avoid pipeline blockage resulting in too much or too little culture solution in the culture tank, which affects the culture results.

[0027] The present invention is further configured as follows: a screw hole is provided at the upper end of the culture plate, a through hole is provided at the sealing plate, a support ring is connected to the lower end of the through hole, a first sealing ring coaxial with the screw hole is provided at the lower end of the support ring, a second sealing ring used in conjunction with the culture plate is provided on the circumferential side of the lower end of the sealing plate, a screw rod is rotatably connected to the culture plate, the lower end of the screw rod is threadedly connected to the screw hole, a handle is connected to the upper end of the screw rod, and a pressing ring rotatably connected to the handle is provided at the upper end of the screw rod and the sealing plate.

[0028] By adopting the above technical solution, the handle is turned to press the pressing ring onto the sealing plate, thereby forming a seal between the sealing plate and the culture plate.

[0029] In summary, the present invention has the following beneficial effects:

[0030] First, the present invention uses a stirring component to evenly stir the culture medium, thereby promoting uniform suspension of cells or organoids in the culture medium and preventing cell precipitation. By uniformly entering and exiting the culture medium, the shear force generated by the liquid flow is controlled, and the blood flow or body fluid flow in the organism is simulated, thereby providing a dynamic 3D environment and providing culture conditions that are closer to reality.

[0031] Secondly, the first insertion tube and the second insertion tube in the present invention are used for the inlet and outlet of the culture medium. They are detachably mounted on the sealing plate, which is convenient for disassembly after use and replacement of the first insertion tube and the second insertion tube, which is conducive to the reuse of other components.

[0032] Third, the weighing sensor in the present invention can monitor the quality of the culture plate in real time. If the water inlet or outlet pipe is blocked, or the water inlet component or water outlet component fails, the quality will change, and timely prevention can avoid unnecessary losses.

[0033] Fourthly, the sealing plate in the present invention is pressed on the culture plate by the pressing ring on the screw rod, which can play a good sealing role and is simple and convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0035] Figure 2 is a cross-sectional view of the culture tank in the present invention;

[0036] Figure 3 is a cross-sectional view of the screw in the present invention;

[0037] Figure 4 is a cross-sectional view of the base in the present invention;

[0038] Figure 5 It is a cross-sectional view of the cover body of the present invention.

[0039] In the figure: 1, base; 11, placement slot; 12, heating element; 13, weighing sensor; 14, cover; 15, germicidal lamp; 2, culture plate; 21, culture tank; 22, screw hole; 31, stirring bar; 32, magnetic stirrer; 4, sealing plate; 41, air pressure sensor; 42, oxygen concentration sensor; 43, carbon dioxide concentration sensor; 44, temperature sensor; 45, through hole; 46, support ring; 47, first sealing ring; 48, second sealing ring; 49, screw; 410, handle; 411, Pressing ring; 51. first insertion tube; 52. first filter screen; 53. water inlet main pipe; 54. water inlet branch pipe; 61. second insertion tube; 62. second filter screen; 63. water outlet main pipe; 64. water outlet branch pipe; 71. first peristaltic pump; 72. culture medium storage tank; 73. second peristaltic pump; 74. waste liquid collection tank; 81. air inlet pipe; 82. air outlet pipe; 83. gas filter; 91. solenoid valve; 92. nitrogen storage tank; 93. carbon dioxide storage tank; 94. third peristaltic pump; 10. control panel. DETAILED DESCRIPTION

[0040] The present invention is further described in detail below in conjunction with the accompanying drawings.

[0041] In the description of the present invention, it is necessary to understand that the directions or positional relationships indicated by terms such as “upper”, “lower”, “left”, “right”, “front”, “back”, “inside” and “outside” are based on the directions or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.

[0042] In addition, the terms "horizontal", "vertical" and the like do not mean that the components are required to be absolutely horizontal or suspended, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0043] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "disposed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, a direct connection, or an indirect connection through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0044] Embodiment, a dynamic 3D embryo suspension culture device, such as Figures 1 to 5 As shown, including base 1, the upper end of base 1 is evenly provided with multiple placement grooves 11, culture plates 2 are placed in the placement grooves 11, and multiple culture plates 21 are evenly provided on the culture plates 2. A stirring assembly for use with the culture plates 2 is provided in the placement grooves 11, and the stirring assembly includes a stirrer 31 and a magnetic stirrer 32, and the magnetic stirrer 32 is provided with multiple and evenly installed at the bottom in the culture grooves 21, and the magnetic stirrer 32 is installed below the culture grooves 21 one by one, and the stirrer 31 is placed in the culture grooves 21. After cells and cell fluid are moved into the culture grooves 21, stirring is carried out by the stirring assembly to promote the uniform suspension of cells in the culture solution and prevent cell precipitation. And the stirring mode using the stirrer 31 and the magnetic stirrer 32 is mainly simple in structure, and the stirrer 31 is easy to clean, and the culture plate 2 can be used as a disposable product without affecting the cost.

[0045] A sealing plate 4 is provided at the upper end of the culture tank 21, and a water inlet pipeline, a water outlet pipeline, an air inlet pipeline and an air outlet pipeline are provided on the sealing plate 4. A water inlet assembly, a water outlet assembly, an air inlet assembly and an air outlet assembly are provided in the base 1. The water inlet assembly is connected to the culture tank 21 through the water inlet pipeline, the water outlet assembly is connected to the culture tank 21 through the water outlet pipeline, the air inlet assembly is connected to the culture tank 21 through the air inlet pipeline, and the air outlet assembly is connected to the culture tank 21 through the air outlet pipeline.

[0046] The water inlet pipeline includes a first insertion tube 51, a first filter screen 52, a water inlet main pipe 53 and a water inlet branch pipe 54. The sealing plate 4 has a hollow structure. A plurality of first insertion tubes 51 are provided and are vertically inserted one by one in the culture tank 21. The first filter screen 52 is installed at the lower end of the first insertion tube 51. The upper end of the first insertion tube 51 is detachably connected to the water inlet branch pipe 54. The water inlet branch pipe 54 is provided in the sealing plate 4. The other end of the water inlet branch pipe 54 is connected to the water inlet main pipe 53. The other end of the water inlet main pipe 53 passes through the sealing plate 4 and is detachably connected to the water inlet component. The water outlet pipeline includes a second insertion tube 61, a second filter 62, a water outlet main pipe 63 and a water outlet branch 64. The second insertion tube 61 is provided with multiple roots and is vertically inserted one by one in the culture tank 21. The first insertion tube 51 and the second insertion tube 61 are installed in the culture tank 21 symmetrically. The second filter 62 is installed at the lower end of the second insertion tube 61. The upper end of the second insertion tube 61 is detachably connected to the water outlet branch 64. The water outlet branch 64 is arranged in the sealing plate 4. The other end of the water outlet branch 64 is connected to the water outlet main pipe 63. The other end of the water outlet main pipe 63 passes through the sealing plate 4 and is detachably connected to the water outlet assembly. The filtration aperture size of the first filter 52 and the second filter 62 is 3-8μm. In this embodiment, the filtration aperture size of the first filter 52 and the second filter 62 is 5μm. Of course, it can also be 3μm, 8μm or any value between 3-8μm, mainly to prevent cells from entering the first insertion tube 51 and the second insertion tube 61.

[0047] The first insertion tube 51 and the second insertion tube 61 are vertically inserted into the culture tank 21. The left-right symmetry is mainly to form a certain flow, control the shear force generated by the liquid flow, and simulate the blood flow or body fluid flow in the organism.

[0048] The water inlet assembly includes a first peristaltic pump 71 and a culture solution storage tank 72, both of which are arranged in the base 1, and the water inlet main pipe 53 is connected to the culture solution storage tank 72 after passing through the first peristaltic pump 71. The water outlet assembly includes a second peristaltic pump 73 and a waste liquid collection tank 74, both of which are installed in the base 1, and the water outlet main pipe 63 is connected to the waste liquid collection tank 74 after passing through the second peristaltic pump 73. The water inlet assembly and the water outlet assembly need to ensure that the flow rate is small and form a microflow, one is to meet the flow demand, and the other is to replace the culture solution to ensure the normal growth of cells.

[0049] The air inlet pipeline includes an air inlet pipe 81 and a gas filter 83. One end of the air inlet pipe 81 is connected to the culture tank 21, and the other end of the air inlet pipe 81 is connected to the air inlet assembly through the gas filter 83. The gas filter 83 can eliminate impurities and pollutants in the gas and allow clean air to enter the culture tank. The filter mesh pore size of the gas filter 83 should be 0.2um. The air outlet pipeline includes an air outlet pipe 82. One end of the air outlet pipe 82 is connected to the culture tank 21, and the other end of the air outlet pipe 82 is connected to the air outlet assembly. The air inlet assembly includes a solenoid valve 91, a nitrogen storage tank 92 and a carbon dioxide storage tank 93, which are all arranged in the base 1. Two air inlet pipes 81 are provided, and one end of the two air inlet pipes 81 away from the sealing plate 4 is respectively connected to the nitrogen storage tank 92 and the carbon dioxide storage tank 93, and the solenoid valve 91 is installed on the air inlet pipe 81; the air outlet assembly includes a third peristaltic pump 94, which is arranged in the base 1, and the air outlet pipe 82 is arranged on the third peristaltic pump 94. The air inlet pipeline, the air outlet pipeline, the air inlet assembly and the air outlet assembly mainly ensure the gas exchange in the culture tank 21, and ensure that the gas concentration in the culture environment is maintained at an optimal level to support the metabolic needs of cells or embryos.

[0050] An air pressure sensor 41, an oxygen concentration sensor 42, a carbon dioxide concentration sensor 43 and a temperature sensor 44 are installed at the lower end of the sealing plate 4, a control panel 10 is provided on the base 1, and the magnetic stirrer 32, the first peristaltic pump 71, the second peristaltic pump 73, the third peristaltic pump, the solenoid valve 91, the air pump, the air pressure sensor 41, the oxygen concentration sensor 42, the carbon dioxide concentration sensor 43 and the temperature sensor 44 are all electrically connected to the control panel 10. The air pressure sensor 41 detects the air pressure in the culture tank 21, the oxygen concentration sensor 42 detects the oxygen concentration in the culture tank 21, the carbon dioxide concentration sensor 43 detects the carbon dioxide concentration in the culture tank 21, and the temperature sensor 44 detects the temperature in the culture tank 21, so as to better control the cell culture.

[0051] A heating element 12 and a weighing sensor 13 are provided at the bottom of the placement tank 11, and both the heating element 12 and the weighing sensor 13 are electrically connected to the control panel 10. It is convenient to heat and weigh the culture plate 2. The heating is to simulate the in vivo environment, and the weighing is to detect the changes in the culture solution in real time to avoid the blockage of the pipeline causing too much or too little culture solution in the culture tank 21, which affects the culture results. An openable cover 14 is provided at the upper end of the base 1, and a sterilizing lamp 15 is provided in the cover 14, and the sterilizing lamp 15 is electrically connected to the control panel 10. The cover 14 and the base 1 form a closed space, which can reduce the heat dissipation, and the sterilizing lamp 15 can sterilize the culture plate 2, sterilize while culturing, and prevent frequent removal of embryos from easy contamination.

[0052] The upper end of the culture plate 2 is provided with a screw hole 22, the sealing plate 4 is provided with a through hole 45, the lower end of the through hole 45 is connected with a support ring 46, the lower end of the support ring 46 is provided with a first sealing ring 47 coaxial with the screw hole 22, the lower end of the sealing plate 4 is provided with a second sealing ring 48 used in conjunction with the culture plate 2, the culture plate 2 is rotatably connected with a screw rod 49, the lower end of the screw rod 49 is threadedly connected to the screw hole 22, the upper end of the screw rod 49 is connected with a handle 410, and the upper end of the screw rod 49 is provided with a pressing ring 411 rotatably connected to the handle 410. By rotating the screw rod 49, the pressing ring 411 is pressed on the sealing plate 4, so that the sealing plate 4 and the culture plate 2 are sealed.

[0053] It should be noted that, in order to ensure the experimental results, the culture plate 2, the first insertion tube 51 and the second insertion tube 61 are disposable items, while other components can be reused after cleaning and disinfection. Of course, if cost is not considered, the sealing plate 4 and the pipeline in the sealing plate 4 can also be disposable items.

[0054] The device is not limited to culturing embryos, but can also culture other cells such as blastocysts or organoids.

[0055] This specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed. However, as long as they are within the scope of the claims of the present invention, they are protected by the patent law.

Claims

1. An embryo dynamic 3D suspension culture device, comprising a base (1), characterized in that: A plurality of placement grooves (11) are evenly arranged at the upper end of the base (1), culture plates (2) are placed in the placement grooves (11), a plurality of culture grooves (21) are evenly arranged on the culture plates (2), a stirring assembly used in conjunction with the culture plates (2) is arranged in the placement grooves (11), a sealing plate (4) is arranged at the upper end of the culture grooves (21), a water inlet pipeline, a water outlet pipeline, an air inlet pipeline and an air outlet pipeline are arranged on the sealing plate (4), a water inlet assembly, a water outlet assembly, an air inlet assembly and an air outlet assembly are arranged in the base (1), the water inlet assembly is connected to the culture groove (21) through the water inlet pipeline, the water outlet assembly is connected to the culture groove (21) through the water outlet pipeline, the air inlet assembly is connected to the culture groove (21) through the air inlet pipeline, and the air outlet assembly is connected to the culture groove (21) through the air outlet pipeline.

2. The embryo dynamic 3D suspension culture device according to claim 1, characterized in that: The stirring assembly includes a stirrer (31) and a magnetic stirrer (32). The magnetic stirrer (32) is provided in multiple and evenly installed at the bottom of the culture tank (21). The magnetic stirrer (32) is installed one by one at the bottom of the culture tank (21), and the stirrer (31) is placed in the culture tank (21).

3. The embryo dynamic 3D suspension culture device according to claim 2, characterized in that: The water inlet pipeline comprises a first insertion tube (51), a first filter screen (52), a water inlet main pipe (53) and a water inlet branch (54); the sealing plate (4) is a hollow structure; a plurality of first insertion tubes (51) are provided and are vertically inserted one by one into the culture tank (21); the first filter screen (52) is installed at the lower end of the first insertion tube (51); the upper end of the first insertion tube (51) is detachably connected to the water inlet branch (54); the water inlet branch (54) is arranged in the sealing plate (4); the other end of the water inlet branch (54) is connected to the water inlet main pipe (53); the other end of the water inlet main pipe (53) passes through the sealing plate (4) and is detachably connected to the water inlet assembly; The water outlet pipeline comprises a second insertion tube (61), a second filter screen (62), a water outlet main pipe (63) and a water outlet branch (64); a plurality of second insertion tubes (61) are provided and are vertically inserted one by one in the culture tank (21); the first insertion tube (51) and the second insertion tube (61) are symmetrically installed in the culture tank (21); the second filter screen (62) is installed at the lower end of the second insertion tube (61); the upper end of the second insertion tube (61) is detachably connected to the water outlet branch (64); the water outlet branch (64) is provided in the sealing plate (4); the other end of the water outlet branch (64) is connected to the water outlet main pipe (63); the other end of the water outlet main pipe (63) passes through the sealing plate (4) and is detachably connected to the water outlet component.

4. The embryo dynamic 3D suspension culture device according to claim 3, characterized in that: The filtering pore sizes of the first filter screen (52) and the second filter screen (62) are both 3-8 μm.

5. The embryo dynamic 3D suspension culture device according to claim 4, characterized in that: The water inlet assembly comprises a first peristaltic pump (71) and a culture solution storage tank (72), wherein the first peristaltic pump (71) and the culture solution storage tank (72) are both arranged in the base (1), and the water inlet main pipe (53) is connected to the culture solution storage tank (72) after passing through the first peristaltic pump (71); The water outlet assembly comprises a second peristaltic pump (73) and a waste liquid collection tank (74); the second peristaltic pump (73) and the waste liquid collection tank (74) are both installed in the base (1); and the water outlet main pipe (63) is connected to the waste liquid collection tank (74) after passing through the second peristaltic pump (73).

6. The embryo dynamic 3D suspension culture device according to claim 5, characterized in that: The air intake pipeline comprises an air intake pipe (81) and a gas filter (83), one end of the air intake pipe (81) is connected to the culture tank (21), and the other end of the air intake pipe (81) is connected to the air intake assembly through the gas filter (83); The gas outlet pipeline comprises a gas outlet pipe (82), one end of the gas outlet pipe (82) is connected to the culture tank (21), and the other end of the gas outlet pipe (82) is connected to the gas outlet component.

7. The embryo dynamic 3D suspension culture device according to claim 6, characterized in that: The air intake assembly comprises a solenoid valve (91), a nitrogen storage tank (92) and a carbon dioxide storage tank (93); the solenoid valve (91), the nitrogen storage tank (92) and the carbon dioxide storage tank (93) are all arranged in the base (1); two air intake pipes (81) are provided; one end of the two air intake pipes (81) away from the sealing plate (4) is respectively connected to the nitrogen storage tank (92) and the carbon dioxide storage tank (93); the solenoid valve (91) is installed on the air intake pipe (81); The air outlet component comprises a third peristaltic pump (94), the third peristaltic pump (94) is arranged in the base (1), and the air outlet pipe (82) is arranged on the third peristaltic pump (94).

8. The embryo dynamic 3D suspension culture device according to claim 7, characterized in that: An air pressure sensor (41), an oxygen concentration sensor (42), a carbon dioxide concentration sensor (43) and a temperature sensor (44) are installed at the lower end of the sealing plate (4); a control panel (10) is provided on the base (1); and the magnetic stirrer (32), the first peristaltic pump (71), the second peristaltic pump (73), the third peristaltic pump, the solenoid valve (91), the air pump, the air pressure sensor (41), the oxygen concentration sensor (42), the carbon dioxide concentration sensor (43) and the temperature sensor (44) are all electrically connected to the control panel (10).

9. The embryo dynamic 3D suspension culture device according to claim 8, characterized in that: A heating element (12) and a weighing sensor (13) are arranged at the bottom of the placement groove (11); the heating element (12) and the weighing sensor (13) are both electrically connected to the control panel (10); an openable cover (14) is arranged at the upper end of the base (1); a germicidal lamp (15) is arranged inside the cover (14); and the germicidal lamp (15) is electrically connected to the control panel (10).

10. The embryo dynamic 3D suspension culture device according to claim 9, characterized in that: The culture plate (2) has a screw hole (22) at its upper end, the sealing plate (4) has a through hole (45), the through hole (45) is connected to a support ring (46) at its lower end, the support ring (46) has a first sealing ring (47) coaxial with the screw hole (22) at its lower end, the sealing plate (4) has a second sealing ring (48) for use with the culture plate (2) at its lower end, the culture plate (2) has a screw rod (49) rotatably connected, the screw rod (49) has a lower end threadedly connected to the screw hole (22), the screw rod (49) has a handle (410) at its upper end, and the screw rod (49) has a pressing ring (411) rotatably connected to the handle (410) at its upper end on the sealing plate (4).

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