Process for activating humic acid by using enzyme preparation

Abstract

The invention relates to the technical field of enzyme preparation application, in particular to a process for activating humic acid by using an enzyme preparation. The process comprises the following steps: acquiring mineral source raw materials, and treating to obtain a raw material mixture; inoculating the EM microbial inoculum into the raw material mixture according to a preset inoculum size, and stirring to obtain a mixture to be fermented; promoting synchronous fermentation of bacteria and enzymes under a preset fermentation condition to form a fermentation product; adding an enzyme preparation into the fermentation product, monitoring gas emission in real time, and determining whether fermentation node detection is performed on the fermentation product or not according to a monitoring result; analyzing the fermentation efficiency, adjusting the preset inoculum size based on an analysis result, and determining whether the fermentation process is finished or not, so as to obtain humic acid. According to the enzyme activity and the actual fermentation condition, the fermentation ending node is accurately judged, the fermentation process is effectively monitored, the fermentation efficiency is analyzed, factors causing low fermentation efficiency are analyzed, corresponding regulation and control measures are adopted, and the fermentation efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of enzyme preparation application, and in particular to a process for activating humic acid by using enzyme preparation. Background Art

[0002] Humic acid is a type of high molecular weight organic compound formed by the decomposition and transformation of plant and animal residues under the action of microorganisms. It is widely present in soil, water and sediments. It has a complex chemical structure and multiple functional groups, showing high biological activity, good adsorption performance and slow release effect. These characteristics of humic acid make it play an important role in improving soil fertility, promoting crop growth, and improving the quality of agricultural products. Due to the slow formation process of natural humic acid and its large structural heterogeneity, these factors limit its wide application in industry. Therefore, by applying a variety of artificial humic acid preparation methods, including microbial decomposition, catalytic / oxidation and hydrothermal methods, the aim is to improve the yield and quality of humic acid while reducing the environmental impact during the preparation process.

[0003] A Chinese patent document with publication number CN109111321B discloses a method for activating humic acid and activated humic acid, comprising: adding humic acid to be activated, phosphoric acid and ammonium nitrate phosphate into an activation reaction device, activating the humic acid with phosphoric acid and ammonium nitrate phosphate, and obtaining activated humic acid and nitrogen oxides; it can be seen that the existing method for activating humic acid lacks effective monitoring of the fermentation process and analysis of the fermentation efficiency, resulting in low fermentation efficiency, and further resulting in low humic acid production. Summary of the invention

[0004] To this end, the present invention provides a process for activating humic acid using an enzyme preparation, so as to overcome the problem in the prior art of lack of real-time monitoring of the fermentation process, resulting in inaccurate addition of the enzyme preparation, low fermentation efficiency and low yield of humic acid.

[0005] To achieve the above object, the present invention provides a process for activating humic acid using an enzyme preparation, comprising: Obtaining mineral raw materials, and processing the mineral raw materials to obtain a raw material mixture; Inoculating the EM bacterial agent into the raw material mixture with a preset inoculation amount, and stirring the mixture to obtain a mixture to be fermented; placing the mixture to be fermented into a sealed fermentation tank and fermenting it under preset fermentation conditions to form a fermentation product; adding an enzyme preparation to the fermentation product in a preset amount, monitoring the gas emission in real time, and determining whether to perform fermentation node detection on the fermentation product according to the monitoring result; Performing fermentation node detection on the fermentation product to analyze the fermentation efficiency, adjusting the preset inoculation amount based on the analysis result, and determining whether the fermentation process is completed in combination with the real-time grayscale difference, thereby obtaining humic acid; Among them, the process of fermentation node detection is to take an image of the side of the fermentation product to obtain a real-time sedimentation image, draw an actual sedimentation curve based on the real-time sedimentation image, and determine whether to control the valve to be closed and whether to add enzyme preparation based on the actual sedimentation curve.

[0006] Further, real-time monitoring of gas emission and determining whether to perform fermentation node detection on the fermentation product according to the monitoring result include: Obtain the gas emission from the fermentation tank, and compare the standard emission with the gas emission: If the gas emission is less than or equal to the standard emission, performing fermentation node detection on the fermentation product; If the gas emission is greater than the standard emission, the fermentation node detection is not performed on the fermentation product, and the gas emission continues to be monitored for determination.

[0007] Further, the fermentation node detection of the fermentation product includes: Taking an initial fermentation image and a continuous sedimentation image of the fermenter; Calculating an initial grayscale value of the initial fermentation image and an average grayscale value of each of the continuous sedimentation images; The ratio of the average gray value corresponding to any continuous sedimentation image to the initial gray value is calculated to obtain the real-time sedimentation rate; Whether the fermentation process is finished is determined according to the real-time precipitation rate and the real-time grayscale difference.

[0008] Further, determining whether the fermentation process is finished according to the real-time precipitation rate and the real-time grayscale difference includes: comparing a standard precipitation rate with the real-time precipitation rate; If the real-time precipitation rate is less than or equal to the standard precipitation rate, obtaining the real-time grayscale difference of the fermentation product, determining whether the fermentation process is finished based on the real-time grayscale difference, and determining whether to add enzyme preparation based on the sampling test result; If the real-time sedimentation rate is greater than the standard sedimentation rate, the preset stirring speed is increased.

[0009] Further, determining whether the fermentation process is finished based on the real-time grayscale difference includes: Calculating the difference between the initial grayscale value and the average grayscale value corresponding to any continuous sedimentation image to obtain a real-time grayscale difference; Compare the standard grayscale difference with the real-time grayscale difference: If the real-time grayscale difference is greater than the standard grayscale difference, an actual sedimentation curve is drawn according to the continuous sedimentation image, and whether to control the valve to be closed is determined according to the actual sedimentation curve; If the real-time grayscale difference is less than or equal to the standard grayscale difference, it is determined that the fermentation process is finished.

[0010] Further, determining whether to control the valve to be closed according to the actual precipitation curve includes: Obtaining the average gray value corresponding to each continuous sedimentation image; The average gray value is plotted over time according to the preset acquisition time, and a linear fit is performed to obtain the average gray value fitting curve; The maximum slope of the average grayscale fitting curve is taken as the image grayscale change rate; Obtaining the image grayscale change rate of each preset acquisition period, and drawing a curve of the change of each image grayscale change rate over time as the actual deposition curve; The change trend of the actual precipitation curve is determined, and when a peak value appears in the actual precipitation curve, the valve is controlled to be closed and the preset stirring speed is increased.

[0011] Furthermore, when no peak appears in the actual precipitation curve, the valve is not controlled to be closed, and whether to add additional enzyme preparation is determined based on the sampling test results.

[0012] Further, determining whether to supplement the enzyme preparation based on the sampling test results includes: The fermentation product is sampled and tested to determine the enzyme activity: If the enzyme activity is greater than the preset standard, increase the preset inoculation amount of the EM inoculant; If the enzyme activity is less than or equal to the preset standard, an enzyme preparation is added to the fermentation product.

[0013] Furthermore, the enzyme preparation comprises cellulase, ligninase or protease.

[0014] Furthermore, processing the mineral raw materials includes: Crushing and washing the mineral raw materials to obtain a raw material mixture; Water is added to the raw material mixture to make the water content of the raw material mixture reach the standard water content.

[0015] Compared with the prior art, the beneficial effect of the present invention lies in that, during the fermentation process, a pre-prepared enzyme preparation is added, and the preset addition amount is 0.5%-2% of the weight of the mineral source raw material. The addition amount is controlled according to the activity of the enzyme, and the temperature, pH value and gas emissions (such as carbon dioxide) during the fermentation process are regularly monitored to ensure the stability of the reaction conditions, and the changes in the fermentation process are recorded for subsequent analysis. According to the activity of the enzyme and the actual fermentation situation, the reaction time is accurately controlled, and the reaction time is generally 7-14 days. By performing sample testing, the fermentation end node is accurately determined, the fermentation process is effectively monitored and the fermentation efficiency is analyzed, the factors leading to low fermentation efficiency are analyzed, and corresponding regulatory measures are taken to improve the fermentation effect.

[0016] Furthermore, by determining that the gas emissions in the fermentation tank have significantly decreased or even approached zero, the fermentation node detection is performed on the fermentation product to accurately determine the fermentation situation, avoid low fermentation efficiency resulting in low gas emissions, and make the monitoring results inaccurate, thereby ensuring the effectiveness of determining whether the fermentation process has ended.

[0017] Furthermore, the color change of the fermentation product is analyzed by image recognition technology, the fermentation process is effectively monitored, the color change and precipitation generation are analyzed to analyze the fermentation efficiency. If it is determined that no peak appears in the actual precipitation curve, it means that the fermentation efficiency is not high. The enzyme activity is then determined to determine whether this is caused by insufficient enzyme addition. If a peak appears in the actual precipitation curve, it means that the formed precipitate is stable. The stirring speed is increased to reduce the aggregation of the precipitate, maintain the fluidity of the mixture, prevent the precipitate from inhibiting the fermentation process, and allow the microorganisms, nutrients and added enzyme preparations to fully contact, thereby improving the fermentation efficiency.

[0018] Furthermore, when it is determined that the enzyme activity is greater than the preset standard, it means that the added amount of the enzyme preparation is sufficient. The reason for the low fermentation efficiency is that the preset inoculation amount is small, resulting in low metabolic activity of the microorganisms. In this way, by increasing the inoculation amount of the EM bacterial agent, the activity of the microorganisms is improved, thereby improving the fermentation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The figure is a schematic diagram of a process for activating humic acid using an enzyme preparation according to an embodiment of the present invention; Figure 2 This is a schematic diagram of a process for processing mineral source raw materials according to an embodiment of the present invention; Figure 3 A schematic diagram of a process for performing fermentation node detection according to an embodiment of the present invention; Figure 4 It is a schematic diagram of a flow chart of determining whether to control the valve to be closed according to an actual sedimentation curve in an embodiment of the present invention. DETAILED DESCRIPTION

[0020] In order to make the objects and advantages of the present invention more clearly understood, the present invention is further described below in conjunction with embodiments; it should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0021] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principles of the present invention and are not intended to limit the protection scope of the present invention.

[0022] It should be noted that, in the description of the present invention, terms such as "up", "down", "left", "right", "inside" and "outside" indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings. This is merely for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention.

[0023] In addition, it should be noted that in the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0024] See also Figure 1 and Figure 2 As shown, Figure 1 The figure is a schematic diagram of a process for activating humic acid using an enzyme preparation according to an embodiment of the present invention; Figure 2 The present invention is a schematic diagram of the process of processing mineral raw materials.

[0025] The present invention provides a process for activating humic acid using an enzyme preparation, comprising: Step S1, obtaining mineral raw materials, and processing the mineral raw materials to obtain a raw material mixture; Step S101, pre-processing, crushing and cleaning the mineral source raw materials to obtain a raw material mixture; Step S102, adjusting the water content by adding water to the raw material mixture so that the water content of the raw material mixture reaches the standard water content; Step S2, inoculating the EM bacterial agent into the raw material mixture with a preset inoculation amount, and stirring to obtain a mixture to be fermented; Step S3, placing the mixture to be fermented into a sealed fermentation tank, and fermenting it under preset fermentation conditions to form a fermentation product; Step S4, enzymatic reaction, opening the valve of the fermentation tank, adding enzyme preparation to the fermentation product in a preset amount, and monitoring the gas emission in real time, and determining whether to perform fermentation node detection on the fermentation product according to the monitoring result; Step S5, performing fermentation node detection on the fermentation product to analyze the fermentation efficiency, adjusting the preset inoculation amount based on the analysis result, and determining whether the fermentation process is completed in combination with the real-time grayscale difference, thereby obtaining the expected activated humic acid; Among them, the process of fermentation node detection is to take an image of the side of the fermentation product to obtain a real-time sedimentation image, draw an actual sedimentation curve based on the real-time sedimentation image, and determine whether to control the valve to be closed and whether to add enzyme preparation based on the actual sedimentation curve.

[0026] The mineral source raw material in this embodiment is weathered coal; during crushing and cleaning, a pulverizer is used to crush the weathered coal to an appropriate particle size (1-5 cm) to improve the contact efficiency between microorganisms and enzymes, and the different raw materials are evenly mixed to ensure uniform distribution of the components; too low moisture will affect the activity of microorganisms, and too high moisture may lead to decay under anaerobic conditions. Therefore, the moisture content of the raw materials is detected to ensure that the moisture content meets the standard. The standard moisture content is between 50%-60%. The moisture content can be adjusted by adding water or by drying the raw materials; EM inoculant is an effective microbial inoculant, and the preset inoculation amount is 1%-5% of the weight of the mineral source raw material. Preferably, the preset inoculation amount is 1% of the weight of the mineral source raw material; gently stir to ensure uniform distribution of the inoculant; the preset fermentation conditions include preset fermentation temperature, preset fermentation humidity and standard pH value, and the preset fermentation temperature is between 30°C and 40°C , the preset fermentation humidity is between 50%-60%, and the standard pH value is between 6.0-7.5 to promote the growth of microorganisms. Preferably, the preset fermentation temperature is 35°C, the preset fermentation humidity is 55%, and the standard pH value is 6.5; during the fermentation process, the pre-prepared enzyme preparation is added, and the preset addition amount is 0.5%-2% of the weight of the mineral source raw material, and the addition amount is controlled according to the activity of the enzyme. The temperature, pH value and gas emissions (such as carbon dioxide) during the fermentation process are regularly monitored to ensure the stability of the reaction conditions, and the changes in the fermentation process are recorded for subsequent analysis. According to the activity of the enzyme and the actual fermentation situation, the reaction time is accurately controlled. The reaction time is generally 7-14 days. By conducting sample testing, the fermentation end node is accurately judged, and the generation of humic acid is timely evaluated. The humic acid is activated by using enzyme preparations to increase the yield and activity of humic acid.

[0027] Specifically, real-time monitoring of gas emissions and determining whether to perform fermentation node detection on the fermentation product according to the monitoring results include: Obtain the gas emission from the fermenter and compare the standard emission with the gas emission: If the gas emission is less than or equal to the standard emission, performing fermentation node detection on the fermentation product in the fermentation tank; If the gas emission is greater than the standard emission, the fermentation node detection is not performed on the fermentation product in the fermentation tank, and the gas emission continues to be monitored for determination.

[0028] In this embodiment, the standard emission amount is the threshold for determining that no gas is produced, and the set value is close to zero. Since microorganisms will produce gases such as carbon dioxide during the fermentation process, if the gas emission amount is significantly reduced or even close to zero, it may indicate that the fermentation process may be completed.

[0029] By determining that the gas emissions in the fermentation tank have significantly decreased or even approached zero, the fermentation node detection is performed on the fermentation product to accurately determine the fermentation situation, avoid low fermentation efficiency resulting in low gas emissions, and make the monitoring results inaccurate, thus ensuring the effectiveness of determining whether the fermentation process has ended.

[0030] See also Figure 3 As shown, it is a schematic diagram of the process of performing fermentation node detection in an embodiment of the present invention; Specifically, the fermentation node detection of the fermentation product in the fermentation tank includes: Step S401, photographing an initial fermentation image and a continuous sedimentation image of the side of the fermentation tank; Step S402, calculating the initial gray value of the initial fermentation image and the average gray value of each continuous sedimentation image; Step S403, calculating the ratio of the average gray value corresponding to any continuous sedimentation image to the initial gray value to obtain the real-time sedimentation rate; Step S404, determining whether the fermentation process is finished according to the real-time precipitation rate and the real-time grayscale difference.

[0031] Specifically, determining whether the fermentation process is finished according to the real-time precipitation rate and the real-time grayscale difference includes: Compare the standard precipitation rate to the real-time precipitation rate: If the real-time precipitation rate is less than or equal to the standard precipitation rate, obtaining the real-time grayscale difference of the fermentation product, determining whether the fermentation process is completed based on the real-time grayscale difference, and determining whether to add enzyme preparation based on the sampling test results; If the real-time sedimentation rate is greater than the standard sedimentation rate, increase the preset stirring speed; Among them, the preset stirring speed is increased to the corrected stirring speed, the corrected stirring speed is the sum of the preset stirring speed and the adjustment step, the adjustment step is the sum of 1 and the increase degree, and the increase degree is the product of the part of the real-time sedimentation rate that exceeds the standard sedimentation rate and the real-time sedimentation rate.

[0032] As the fermentation process approaches the end, the color of the fermentation product becomes darker and precipitation occurs. Therefore, in this embodiment, the precipitation rate is characterized by calculating the percentage change of the color of the fermentation product relative to the initial color. The standard precipitation rate is set to 130%, which means that when the percentage change of the fermentation product relative to the initial color reaches 130%, the color of the fermentation product has become darker, and the fermentation process is progressing well. The initial gray value is 100. By analyzing the gray value of each pixel, the average gray value of the fermentation product is calculated. When it is determined that the real-time precipitation rate is greater than the standard precipitation rate, it means that the average gray value has reached or exceeded The set threshold is 200. If the average gray value reaches or exceeds 200, it means that the fermentation process is good. For example, during the fermentation process, after a period of time, the monitored average gray value is 230, and the real-time precipitation rate is calculated to be [(230-100) / 100]×100%=130%, indicating that the color of the fermentation product has become darker, the fermentation process is progressing well, and the fermentation is completed. The color change is monitored in real time to analyze the precipitation process, and the precipitation formation in the fermentation process is discovered in time. By monitoring the color change rate, the operating parameters are adjusted in time to optimize the fermentation process and improve the fermentation efficiency.

[0033] Specifically, determining whether the fermentation process is finished based on the real-time grayscale difference includes: Calculating the difference between the initial grayscale value and the average grayscale value corresponding to any continuous sedimentation image to obtain a real-time grayscale difference; Compare the standard grayscale difference with the real-time grayscale difference: If the real-time grayscale difference is greater than the standard grayscale difference, an actual sedimentation curve is drawn according to the continuous sedimentation image, and whether to control the valve to be closed is determined according to the actual sedimentation curve; If the real-time grayscale difference is less than or equal to the standard grayscale difference, the fermentation process is determined to be over; The standard grayscale difference value set in this embodiment is 230.

[0034] See also Figure 4 As shown, it is a schematic diagram of a flow chart of determining whether to control the valve to be closed according to an actual precipitation curve in an embodiment of the present invention; Specifically, determining whether to control the valve to be closed according to the actual precipitation curve includes: Step S501, obtaining the average gray value corresponding to each continuous sedimentation image; Step S502, plotting a curve of average gray value changing over time with a preset acquisition time, and performing linear fitting to obtain an average gray value fitting curve; Step S503, taking the maximum slope of the average grayscale fitting curve as the image grayscale change rate; Step S504, obtaining the image grayscale change rate of each preset acquisition period, and drawing a curve of the grayscale change rate of each image over time as the actual deposition curve; Step S505, determining the change trend of the actual precipitation curve, and when a peak appears in the actual precipitation curve, controlling the valve to close and increasing the preset stirring speed; When no peak appears in the actual precipitation curve, the valve is not controlled to be closed, and whether to add the enzyme preparation is determined based on the sampling test result; Among them, the preset stirring speed is increased to 1.1 times the preset stirring speed; In this embodiment, the preset acquisition duration is set to 5 seconds; the preset acquisition interval is 1 second; Image recognition technology is used to analyze the color changes of fermentation products, effectively monitor the fermentation process, analyze color changes and precipitation generation, and analyze the fermentation efficiency. If it is determined that there is no peak in the actual precipitation curve, it means that the fermentation efficiency is not high. The enzyme activity is then determined to determine whether this is caused by insufficient enzyme addition. If a peak appears in the actual precipitation curve, it means that the formed precipitate is stable. At this time, humic acid exists in the form of precipitation. The stirring speed is increased to reduce the aggregation of precipitates, maintain the fluidity of the mixture, prevent the precipitate from inhibiting the fermentation process, and allow microorganisms, nutrients and added enzyme preparations to fully contact, thereby improving the fermentation efficiency.

[0035] Specifically, the determination of whether to add enzyme preparations based on the sampling test results includes: The fermentation product is sampled and tested to determine the enzyme activity: If the enzyme activity is greater than the preset standard, increase the preset inoculum amount of the EM inoculant to the corrected inoculum amount; If the enzyme activity is less than or equal to a preset standard, adding an enzyme preparation to the fermentation product; Among them, the revised inoculation amount is 1.5 times the preset inoculation amount; the supplementary enzyme preparation is 1.2 times the preset addition amount.

[0036] In this embodiment, colorimetry, fluorescence or other biochemical detection methods are used to evaluate the activity of the enzyme. If the enzyme additive is cellulase, the preset standard is set to 10 U / mL. Since excessive addition of enzyme preparations may increase the viscosity of the mixture and affect the fluidity and fermentation efficiency of the mixture, unnecessary addition of enzyme preparations can be effectively avoided by monitoring the enzyme activity and precipitation formation, the amount of enzyme preparations added can be effectively controlled to ensure efficient fermentation.

[0037] When the enzyme activity is judged to be greater than the preset standard, it means that the added amount of enzyme preparation is sufficient. The reason for the low fermentation efficiency is that the preset inoculation amount is small, resulting in low metabolic activity of the microorganisms. In this case, by increasing the inoculation amount of the EM bacterial agent, the activity of the microorganisms is improved, thereby improving the fermentation efficiency.

[0038] Example 1, the enzyme preparation is cellulase, the raw material is 500 grams of weathered coal; the preset inoculation amount of the EM bacterial agent is 10 grams (2%), the preset addition amount of cellulase is 15g (3%), the fermentation conditions are: temperature is 35°C, pH value is 6.5, humidity is 55%, and the fermentation time is 8 days.

[0039] Example 2, the enzyme preparation is ligninase, the raw material is 500 grams of weathered coal; the preset inoculation amount of EM fungus is 10 grams (2%), the preset addition amount of ligninase is 15g (3%), the fermentation conditions are: temperature is 35°C, pH value is 6.5, humidity is 55%, and fermentation time is 9 days.

[0040] Example 3, the enzyme preparation is protease, the raw material is 500 grams of weathered coal; the preset inoculation amount of the EM bacterial agent is 10 grams (2%), the preset addition amount of protease is 15g (3%), the fermentation conditions are: temperature is 35°C, pH value is 6.5, humidity is 55%, and the fermentation time is 10 days.

[0041] Table 1 ; Table 1 shows the experimental results of activating humic acid with different enzyme preparations. It can be seen from Table 1 that different enzyme preparations have a significant effect on the production of humic acid, among which cellulase has the best effect. It provides experimental data that can confirm the successful preparation of a higher content of humic acid, proves the effectiveness of the process, and provides a control test.

[0042] Specifically, the humic acid samples and raw weathered coal samples obtained according to the above embodiments of the present invention were tested, and the humic acid content, free humic acid content, organic matter and moisture were tested. The experimental data and experimental results are as follows: The experimental data are shown in Table 2, which is the test comparison results of humic acid samples and raw weathered coal samples obtained from different batches of the above embodiments. Sample 1 is the raw weathered coal, and sample 2 is the products of different batches corresponding to the enzyme preparation being cellulase.

[0043] Table 2 ; It has been found through testing that the preparation method of this embodiment can increase the free acid content in humic acid (the raw material is weathered coal) to prepare a high-performance soil conditioner.

[0044] So far, the technical solutions of the present invention have been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

[0045] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A process for activating humic acid using an enzyme preparation, characterized in that: include, Obtaining mineral raw materials, and processing the mineral raw materials to obtain a raw material mixture; Inoculating the EM bacterial agent into the raw material mixture with a preset inoculation amount, and stirring the mixture to obtain a mixture to be fermented; placing the mixture to be fermented into a sealed fermentation tank and fermenting it under preset fermentation conditions to form a fermentation product; adding an enzyme preparation to the fermentation product in a preset amount, monitoring the gas emission in real time, and determining whether to perform fermentation node detection on the fermentation product according to the monitoring result; Performing fermentation node detection on the fermentation product to analyze the fermentation efficiency, adjusting the preset inoculation amount based on the analysis result, and determining whether the fermentation process is completed in combination with the real-time grayscale difference, thereby obtaining humic acid; Among them, the process of fermentation node detection is to take an image of the side of the fermentation product to obtain a real-time sedimentation image, draw an actual sedimentation curve based on the real-time sedimentation image, and determine whether to control the valve to be closed and whether to add enzyme preparations based on the actual sedimentation curve.

2. The process for activating humic acid using an enzyme preparation according to claim 1, characterized in that: Real-time monitoring of gas emissions and determining whether to perform fermentation node detection on the fermentation product according to the monitoring results include: Obtain the gas emission from the fermentation tank, and compare the standard emission with the gas emission: If the gas emission is less than or equal to the standard emission, performing fermentation node detection on the fermentation product; If the gas emission is greater than the standard emission, the fermentation node detection is not performed on the fermentation product, and the gas emission continues to be monitored for determination.

3. The process for activating humic acid using an enzyme preparation according to claim 2, characterized in that: The fermentation node detection of the fermentation product includes: Taking an initial fermentation image and a continuous sedimentation image of the fermenter; Calculating an initial grayscale value of the initial fermentation image and an average grayscale value of each of the continuous sedimentation images; The ratio of the average gray value corresponding to any continuous sedimentation image to the initial gray value is calculated to obtain the real-time sedimentation rate; Whether the fermentation process is finished is determined according to the real-time precipitation rate and the real-time grayscale difference.

4. The process for activating humic acid using an enzyme preparation according to claim 3, characterized in that: Determining whether the fermentation process is finished according to the real-time precipitation rate and the real-time grayscale difference includes: comparing a standard precipitation rate with the real-time precipitation rate; If the real-time precipitation rate is less than or equal to the standard precipitation rate, obtaining the real-time grayscale difference of the fermentation product, determining whether the fermentation process is finished based on the real-time grayscale difference, and determining whether to add enzyme preparation based on the sampling test result; If the real-time sedimentation rate is greater than the standard sedimentation rate, the preset stirring speed is increased.

5. The process for activating humic acid using an enzyme preparation according to claim 4, characterized in that: Determining whether the fermentation process is finished based on the real-time grayscale difference includes: Calculating the difference between the initial grayscale value and the average grayscale value corresponding to any continuous sedimentation image to obtain a real-time grayscale difference; Compare the standard grayscale difference with the real-time grayscale difference: If the real-time grayscale difference is greater than the standard grayscale difference, an actual sedimentation curve is drawn according to the continuous sedimentation image, and whether to control the valve to be closed is determined according to the actual sedimentation curve; If the real-time grayscale difference is less than or equal to the standard grayscale difference, it is determined that the fermentation process is finished.

6. The process for activating humic acid using an enzyme preparation according to claim 5, characterized in that: Determining whether to control the valve to close according to the actual precipitation curve includes: Obtaining the average gray value corresponding to each continuous sedimentation image; The average gray value is plotted over time according to the preset acquisition time, and a linear fit is performed to obtain the average gray value fitting curve; The maximum slope of the average grayscale fitting curve is taken as the image grayscale change rate; Obtaining the image grayscale change rate of each preset acquisition period, and drawing a curve of the change of each image grayscale change rate over time as the actual deposition curve; The change trend of the actual precipitation curve is determined, and when a peak value appears in the actual precipitation curve, the valve is controlled to be closed and the preset stirring speed is increased.

7. The process for activating humic acid using an enzyme preparation according to claim 6, characterized in that: When no peak appears in the actual precipitation curve, the valve is not controlled to be closed, and whether to add additional enzyme preparation is determined based on the sampling test results.

8. The process for activating humic acid using an enzyme preparation according to claim 4, characterized in that: Determine whether to add enzyme preparations based on the sampling test results, including: The fermentation product is sampled and tested to determine the enzyme activity: If the enzyme activity is greater than the preset standard, increase the preset inoculation amount of the EM inoculant; If the enzyme activity is less than or equal to the preset standard, an enzyme preparation is added to the fermentation product.

9. The process for activating humic acid using an enzyme preparation according to claim 1, characterized in that: The enzyme preparation comprises cellulase, ligninase or protease.

10. The process for activating humic acid using an enzyme preparation according to claim 1, characterized in that: Processing the mineral raw materials includes: Crushing and washing the mineral raw materials to obtain a raw material mixture; Water is added to the raw material mixture to make the water content of the raw material mixture reach the standard water content.

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