A sampling and detection device for preparing a collagen matrix

Abstract

The present invention discloses a sampling and detection device for the preparation of collagen matrix, which relates to the field of collagen matrix sampling. It includes a base, a rotating base, a robotic arm, and a cylinder. A rotating base driven by a motor is installed on the base. A robotic arm is fixed on the rotating base. A cylinder is fixed on the robotic arm. A limiting mechanism is fixed at the output end of the cylinder. A sampling mechanism is fixed at the lower end of the limiting mechanism. A scraping mechanism is arranged outside the sampling mechanism. For this sampling and detection device for the preparation of collagen matrix, after sampling is completed, when the sampling mechanism separates from the liquid surface of the collagen matrix, the cylinder contracts to drive the convex shaft to move. With the sliding action between the convex shaft and the spiral structure of the chute, the scraper rotates around the outer wall of the sampling bucket, realizing the scraping of the collagen matrix on the outer wall of the sampling bucket, avoiding waste caused by the collagen matrix adhered to the outer wall of the sampling bucket, and the whole operation does not require manual handling, improving the convenience of using the device.

Description

Technical Field

[0001] The present invention relates to the technical field of collagen matrix sampling, and specifically to a sampling and detection device for collagen matrix preparation. Background Art

[0002] Collagen matrix is a substance widely used in the biomedical field. It has the advantages of promoting cell growth and repair, low immunogenicity, good biodegradability, and excellent hemostatic effect. After the production of collagen matrix is completed, sampling and detection are required to determine the production quality of the collagen matrix.

[0003] At the present stage, when sampling collagen matrix, since the collagen matrix is a colloidal substance, manual extraction through a syringe is required during the sampling process. After sampling, a large amount of collagen matrix will adhere to the outer wall of the syringe and cannot be recycled, resulting in waste of the collagen matrix. Therefore, in view of the above problems, a sampling and detection device for collagen matrix preparation is designed to better meet the actual use requirements. Summary of the Invention

[0004] The purpose of the present invention is to provide a sampling and detection device for collagen matrix preparation to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A sampling and detection device for collagen matrix preparation, including a base, a rotating seat, a robotic arm, and a cylinder. A rotating seat driven by a motor is installed on the base. A robotic arm is fixed on the rotating seat. A cylinder is fixed on the robotic arm. A limiting mechanism is fixed at the output end of the cylinder. A sampling mechanism is fixed at the lower end of the limiting mechanism. A scraping mechanism is arranged outside the sampling mechanism. The scraping mechanism includes a scraper, a connecting rod, a convex shaft, a chute, a cylindrical rod, a limiting rod, and a guide ring. The scraper is in sliding contact with the outer wall of the sampling bucket. A connecting rod is fixed on the scraper. A convex shaft is fixed at the upper end of the connecting rod. The convex shaft is in sliding connection with the chute. The chute is opened on the cylindrical rod, and the upper side of the chute is a spiral structure, and the lower side of the chute is a strip structure. The cylindrical rod is fixed on the cylinder. A limiting rod is also fixed on the connecting rod, and the end of the limiting rod is a spherical structure, and the spherical structure at the end of the limiting rod is nested in the groove opened on the guide ring for sliding. The guide ring is fixed on the fixed box. When the cylinder extends, the limiting mechanism and the sampling mechanism are driven to move for sampling operation. After sampling is completed, when the cylinder contracts and the sampling mechanism is separated from the liquid level of the collagen matrix, at this time, the convex shaft just cooperates with the spiral groove on the upper side of the chute. By continuously contracting the cylinder and cooperating with the sliding action between the convex shaft and the chute, the scraper can be rotated. Through the sliding action between the scraper and the sampling bucket, the collagen matrix remaining on the outer wall of the sampling bucket can be scraped off.

[0006] Preferably, the limiting mechanism includes a fixed box, a contact sensor, a cross bar, a first spring and a clamping block. The fixed box is fixed to the output end of the cylinder, and the contact sensor is fixed inside the fixed box. The cross bar is slidably connected to the fixed box. Through the function of the contact sensor, it can provide a basic guarantee for the normal operation of the device.

[0007] Preferably, a first spring is fixed between the cross bar and the fixed box, and a clamping block is also fixed on the cross bar. Through the elastic action of the first spring, it can provide a basic acting force for the automatic reset of the clamping block and ensure the normal operation of the device.

[0008] Preferably, the sampling mechanism includes a sampling bucket, a sample storage chamber, an electric valve, a liquid inlet, a vertical rod, a piston head, a return spring, a cambered surface block and a push rod. The sampling bucket is fixed to the lower end of the fixed box by bolts, and a plurality of sample storage chambers are equidistantly arranged in the sampling bucket. The sample storage chambers communicate with the electric valve fixed on the sampling bucket, and the sample storage chambers are connected to the liquid inlet. A sealing mechanism is fixed in the liquid inlet. Through the functions of the sample storage chambers and the liquid inlet, it can ensure the normal sampling. With the cooperation of the electric valve, it can balance the internal air pressure of the sample storage chambers and ensure the normal operation of the device.

[0009] Preferably, a vertical rod is slidably connected to the sampling bucket, and a plurality of piston heads are equidistantly fixed on the vertical rod. The piston heads and the sample storage chambers are arranged in one-to-one correspondence and are slidably connected. A return spring is also fixed between the piston heads and the sample storage chambers. Through the sliding action between the piston heads and the sample storage chambers, the suction of the sample can be realized to ensure the normal sampling. And through the elastic action of the return spring, it can provide a basic guarantee for the automatic discharge of the sample later.

[0010] Preferably, a cambered surface block is fixed to the upper end of the vertical rod, and the cambered surface block is engaged with the clamping block. A push rod is also fixed to the upper end of the cambered surface block. Through the engagement between the cambered surface block and the clamping block, the positioning of the vertical rod and the piston head can be realized to ensure the normal operation of the device.

[0011] Preferably, the push rod is located directly below the contact sensor, and the heights of the push rod and the cambered surface block are equal to the distance between the clamping block and the contact sensor. Through the functions of the push rod and the contact sensor, it can provide a basic guarantee for the automatic control of the device.

[0012] Preferably, the sealing mechanism includes a partition board, a sealing plug, a fixed rod, a fixing plate, a sliding rod and a second spring. The partition board is fixed in the liquid inlet, and the partition board and the sealing plug are nested. Through the sealing between the partition board and the sealing plug, the overflow of the sample can be effectively avoided.

[0013] Preferably, the sealing plug is fixed to one end of the fixing rod, and the other end of the fixing rod is fixed to the fixing plate. A sliding rod is fixed to the fixing plate, and the sliding rod is slidably connected to the partition. When the sealing plug and the fixing plate move, the sliding guiding function between the sliding rod and the partition can ensure the stability of the movement of the sealing plug and the fixing plate.

[0014] Preferably, one end of the fixing plate is fixedly connected to one end of the second spring, and the other end of the second spring is fixed to the partition. Through the elastic action of the second spring, a basic acting force can be provided for the automatic reset of the fixing plate and the sealing plug.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0016] 1. For the sampling and detection device for preparing collagen matrix, after sampling is completed, when the sampling mechanism is separated from the liquid surface of the collagen matrix, the cylinder contracts to drive the convex shaft to move. With the sliding action between the convex shaft and the spiral structure of the chute, the scraper rotates around the outer wall of the sampling bucket, realizing the scraping of the collagen matrix on the outer wall of the sampling bucket, avoiding waste caused by the collagen matrix adhered to the outer wall of the sampling bucket, and the whole operation does not require manual treatment, improving the convenience of using the device;

[0017] 2. For the sampling and detection device for preparing collagen matrix, during sampling, the cylinder can be used to make the limiting mechanism and the sampling mechanism enter the storage container. With the cooperation of the vertical rod, the piston head, the electric valve, and the liquid inlet, the sample can be extracted at multiple positions, thus realizing the sampling function. After sampling is completed, through the action of the cross bar, the first spring, the clamping block, and the arc-shaped block, the vertical rod can be locked to avoid sample discharge, ensuring the normal progress of sampling. During the whole sampling process, it is controlled by the robotic arm and the cylinder, without manual intervention, reducing the labor intensity of workers. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a front three-dimensional structure schematic diagram of the overall composition of the device of the present invention;

[0019] Figure 2 It is a front view structure schematic diagram of the cylinder, the limiting mechanism, and the sampling mechanism of the present invention;

[0020] Figure 3 It is a front three-dimensional structure schematic diagram of the novel structure of the cross-section of the limiting mechanism and the scraping mechanism of the present utility model;

[0021] Figure 4 It is a front three-dimensional structure schematic diagram of the limiting mechanism of the present invention;

[0022] Figure 5 For the present invention Figure 4 The enlarged structure schematic diagram at A in;

[0023] Figure 6 This is a front view sectional three-dimensional structure schematic diagram of the sampling mechanism of the present invention;

[0024] Figure 7 This is a three-dimensional structure schematic diagram of the composition of the sealing mechanism of the present invention.

[0025] In the figure: 1, base; 2, rotating seat; 3, robotic arm; 4, cylinder; 5, limiting mechanism; 501, fixed box; 502, contact sensor; 503, cross bar; 504, first spring; 505, clamping block; 6, sampling mechanism; 601, sampling bucket; 602, sample storage cavity; 603, electric valve; 604, liquid inlet; 605, vertical rod; 606, piston head; 6061, return spring; 607, arc-shaped block; 608, ejector rod; 7, sealing mechanism; 701, partition board; 702, sealing plug; 703, fixed rod; 704, fixed plate; 705, sliding rod; 706, second spring; 8, scraping mechanism; 801, scraper; 802, connecting rod; 803, convex shaft; 804, sliding groove; 805, cylindrical rod; 806, limiting rod; 807, guide ring. Detailed implementation manners

[0026] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0027] Please refer to Figures 1 - 7, the present invention provides a technical solution: a sampling and detection device for preparing a collagen matrix, including a base 1, a rotating base 2, a robotic arm 3 and a cylinder 4. A rotating base 2 driven by a motor is installed on the base 1. A robotic arm 3 is fixed on the rotating base 2. A cylinder 4 is fixed on the robotic arm 3. A limiting mechanism 5 is fixed at the output end of the cylinder 4. A sampling mechanism 6 is fixed at the lower end of the limiting mechanism 5. A scraping mechanism 8 is arranged outside the sampling mechanism 6. The scraping mechanism 8 includes a scraping plate 801, a connecting rod 802, a convex shaft 803, a sliding groove 804, a cylindrical rod 805, a limiting rod 806 and a guiding ring 807. The scraping plate 801 contacts and slides on the outer wall of the sampling bucket 601. A connecting rod 802 is fixed on the scraping plate 801. A convex shaft 803 is fixed at the upper end of the connecting rod 802. The convex shaft 803 is slidably connected with the sliding groove 804. The sliding groove 804 is opened on the cylindrical rod 805. The upper side of the sliding groove 804 is a spiral structure, and the lower side of the sliding groove 804 is a strip structure. The cylindrical rod 805 is fixed on the cylinder 4. A limiting rod 806 is also fixed on the connecting rod 802. The end of the limiting rod 806 is a spherical structure, and the spherical structure at the end of the limiting rod 806 is nested in the groove opened on the guiding ring 807 for sliding. The guiding ring 807 is fixed on the fixed box 501. When the cylinder 4 extends, the limiting mechanism 5 and the sampling mechanism 6 are driven to move for sampling operation. After sampling is completed, when the cylinder 4 contracts and the sampling mechanism 6 is separated from the collagen matrix liquid surface, at this time, the convex shaft 803 just cooperates with the spiral groove on the upper side of the sliding groove 804. By continuously contracting the cylinder 4 and cooperating with the sliding action between the convex shaft 803 and the sliding groove 804, the scraping plate 801 can be rotated. Through the sliding action between the scraping plate 801 and the sampling bucket 601, the collagen matrix remaining on the outer wall of the sampling bucket 601 can be scraped off.

[0028] When using this sampling and detection device for preparing a collagen matrix, as Figures 1 - 7 shown, when sampling, through the action of the rotating base 2 and the robotic arm 3, the positions of the cylinder 4 and the sampling mechanism 6 can be adjusted to ensure that the distance from the lower end surface position of the sampling mechanism 6 to the collagen matrix liquid surface is greater than the height of the threaded groove on the sliding groove 804. After the position of the sampling mechanism 6 is adjusted, by controlling the cylinder 4 to extend, the limiting mechanism 5, the sampling mechanism 6 and the convex shaft 803 are driven to move downward. When the convex shaft 803 moves from the threaded groove on the sliding groove 804 to the strip groove on the sliding groove 804, at this time, the sampling mechanism 6 does not contact the collagen matrix liquid surface, thus avoiding stirring the collagen matrix when the convex shaft 803 slides in the threaded groove on the sliding groove 804 and drives the scraping plate 801 to rotate, and ensuring the normal progress of subsequent sampling;

[0029] The sampling mechanism 6 includes a sampling bucket 601, a sample storage chamber 602, an electric valve 603, a liquid inlet 604, a vertical rod 605, a piston head 606, a return spring 6061, an arc-shaped block 607, and a push rod 608. The sampling bucket 601 is fixed to the lower end of the fixed box 501 by bolts, and a plurality of sample storage chambers 602 are equidistantly arranged in the sampling bucket 601. The sample storage chamber 602 communicates with the electric valve 603 fixed to the sampling bucket 601, and at the same time, the sample storage chamber 602 is connected to the liquid inlet 604. A sealing mechanism 7 is fixed in the liquid inlet 604. A vertical rod 605 is slidably connected to the sampling bucket 601, and a plurality of piston heads 606 are equidistantly fixed on the vertical rod 605. The piston heads 606 are distributed in a one-to-one correspondence with the sample storage chambers 602, and at the same time, the piston heads 606 are slidably connected to the sample storage chambers 602. A return spring 6061 is also fixed between the piston heads 606 and the sample storage chambers 602. The upper end of the vertical rod 605 is fixed with an arc-shaped block 607, and the arc-shaped block 607 is snap-connected to the clamping block 505. The upper end of the arc-shaped block 607 is also fixed with a push rod 608. The push rod 608 is located directly below the contact sensor 502, and the heights of the push rod 608 and the arc-shaped block 607 are equal to the distance between the clamping block 505 and the contact sensor 502. The sealing mechanism 7 includes a partition plate 701, a sealing plug 702, a fixing rod 703, a fixing plate 704, a sliding rod 705, and a second spring 706. The partition plate 701 is fixed in the liquid inlet 604, and the partition plate 701 is nested with the sealing plug 702. The sealing plug 702 is fixed to one end of the fixing rod 703, and the other end of the fixing rod 703 is fixed to the fixing plate 704. A sliding rod 705 is fixed to the fixing plate 704, and at the same time, the sliding rod 705 is slidably connected to the partition plate 701. The fixing plate 704 is fixed to one end of the second spring 706, and the other end of the second spring 706 is fixed to the partition plate 701.

[0030] When the air cylinder 4 continues to extend and drives the limit mechanism 5, the sampling mechanism 6, and the convex shaft 803 to move downward, as Figures 1 - 7As shown in the figure, the scraper 801 does not rotate at this time. During the downward movement of the sampling mechanism 6, when the vertical rod 605 contacts the bottom surface of the collagen matrix storage container, the electric valve 603 opens at this time to facilitate the balance of the air pressure at the upper end of the sample storage cavity 602. And the vertical rod 605 slides upward relative to the sampling bucket 601 under the force, thereby driving the piston head 606 to slide upward in the sample storage cavity 602, so that a negative pressure is formed between the sample storage cavity 602 and the space below the piston head 606. Under the action of the air pressure, the sealing plug 702 in the liquid inlet 604 is forced to move. With the sliding guiding effect between the sliding rod 705 and the partition 701, the stability of the movement of the sealing plug 702 can be ensured. When the sealing plug 702 separates from the opening on the partition 701, the sealing effect is released. At this time, under the negative pressure in the space between the sample storage cavity 602 and the space below the piston head 606, the collagen matrix enters the sample storage cavity 602 through the liquid inlet 604 for storage, thereby realizing the sampling function. And through the action of multiple sample storage cavities 602, the sampling of collagen matrix at different positions in the collagen matrix storage container can be realized, so as to ensure the accuracy of subsequent detection data;

[0031] The limiting mechanism 5 includes a fixed box 501, a contact sensor 502, a cross bar 503, a first spring 504 and a clamping block 505. The fixed box 501 is fixed to the output end of the air cylinder 4, and the contact sensor 502 is fixed in the fixed box 501, and the cross bar 503 is slidably connected to the fixed box 501; a first spring 504 is fixed between the cross bar 503 and the fixed box 501, and a clamping block 505 is also fixed on the cross bar 503;

[0032] When the vertical rod 605 slides upward relative to the sampling bucket 601 under the force, as Figures 1 - 7 shown, the vertical rod 605 moves synchronously to drive the arc-shaped block 607 and the ejector rod 608 to move. When the arc-shaped block 607 contacts and slides with the inclined surface of the clamping block 505, the clamping block 505 is forced to move at this time. With the sliding guiding effect between the cross bar 503 and the fixed box 501, the stability of the movement of the clamping block 505 can be ensured. When the ejector rod 608 contacts the contact sensor 502, the contact sensor 502 transmits a signal to the controller, so that the air cylinder 4 stops extending, and the electric valve 603 closes at this time, thus completing the sampling operation. When the pressure in the space below the piston head 606 is balanced, at this time, under the action of the second spring 706, the sealing plug 702 is reset to seal the opening on the partition 701 to prevent the sample from overflowing. And at this time, the arc-shaped block 607 moves above the clamping block 505. With the elastic action of the first spring 504, the clamping block 505 is reset to engage with the lower end surface of the arc-shaped block 607, thereby realizing the positioning function of the vertical rod 605;

[0033] After the sampling is completed, as Figures 1 - 7As shown in the figure, by controlling the contraction of the cylinder 4, the limiting mechanism 5, the sampling mechanism 6 and the convex shaft 803 are driven to move upward. When the lower end surface of the sampling bucket 601 is separated from the liquid level of the collagen matrix, at this time, the convex shaft 803 slides in the strip groove on the sliding groove 804. When the cylinder 4 continues to contract and drives the convex shaft 803 to move upward into the spiral groove on the sliding groove 804 for sliding, it can drive the scraper 801 and the connecting rod 802 to rotate. With the sliding action of the spherical structure at the end of the limiting rod 806 nested between the slots of the guiding ring 807, the stability of the rotation of the scraper 801 and the connecting rod 802 can be ensured. Through the rotation of the scraper 801, the collagen matrix adhered to the outer surface of the sampling bucket 601 can be scraped off, and the scraped collagen matrix falls into the collagen matrix storage container, thus avoiding the waste of the collagen matrix. It is sufficient until the cylinder 4 and the convex shaft 803 return to the initial position, thereby completing the sampling operation. Subsequently, when removing the sample, only need to open the electric valve 603 and then pull the cross bar 503, so that the clamping block 505 is separated from the arc-shaped block 607, thereby releasing the limiting effect. With the action of the return spring 6061, the piston head 606 slides downward in the sample storage cavity 602, thereby extruding the sample stored in the sample storage cavity 602 for subsequent detection. Moreover, when the sample is extruded, under the action of the extrusion pressure, the sealing plug 702 can be moved to separate from the partition plate 701, releasing the sealing effect and facilitating the normal discharge of the sample. This is the working principle of the sampling and detection device for the preparation of the collagen matrix.

[0034] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made in these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A sampling and detection device for preparing a collagen matrix, comprising a base (1), a rotating base (2), a mechanical arm (3) and a cylinder (4), wherein the base (1) is mounted with a rotating base (2) driven by a motor, the rotating base (2) is fixed with a mechanical arm (3), and the mechanical arm (3) is fixed with a cylinder (4), characterized in that: The output end of the cylinder (4) is fixed with a limiting mechanism (5), the lower end of the limiting mechanism (5) is fixed with a sampling mechanism (6), the outer side of the sampling mechanism (6) is provided with a scraping mechanism (8), the scraping mechanism (8) comprises a scraper (801), a connecting rod (802), a convex shaft (803), a slide groove (804), a cylindrical rod (805), a limiting rod (806) and a guide ring (807), the scraper (801) is in contact and slides with the outer wall of the sampling barrel (601), the connecting rod (802) is fixed on the scraper (801), the upper end of the connecting rod (802) is fixed with a convex shaft (803), the convex shaft (803) and the slide groove (804) are in sliding connection, and the slide groove (804) is provided on the cylindrical rod ( The cylindrical rod (805) is fixed on the cylinder (4), and the upper side of the slide groove (804) is a spiral structure, and the lower side of the slide groove (804) is a strip structure. The cylindrical rod (805) is fixed on the cylinder (4). The connecting rod (802) is also fixed with a limiting rod (806), and the end of the limiting rod (806) is a spherical structure, and the spherical structure at the end of the limiting rod (806) is nested in a groove on a guide ring (807) for sliding. The guide ring (807) is fixed on the fixed box (501). The cylinder (4) extends to drive the limiting mechanism (5) and the sampling mechanism (6) to move to perform a sampling operation. The limiting mechanism (5) includes a fixed box (501), a contact sensor (502), a cross bar (503), a first spring (504) and a clamping block (5 05), the fixed box (501) is fixed to the output end of the cylinder (4), and a contact sensor (502) is fixed in the fixed box (501), and a cross bar (503) is slidably connected to the fixed box (501), a first spring (504) is fixed between the cross bar (503) and the fixed box (501), and a clamping block (505) is also fixed on the cross bar (503), the sampling mechanism (6) comprises a sampling barrel (601), a sample storage chamber (602), an electric valve (603), a liquid inlet (604), a vertical rod (605), a piston head (606), a return spring (6061), a curved surface block (607) and a push rod (608), and the sampling barrel (601) is fixed to the bottom of the fixed box (501) by bolts. end, and sample storage chambers (602) are evenly spaced in the sampling barrel (601), and the sample storage chambers (602) are connected to an electric valve (603) fixed on the sampling barrel (601), and the sample storage chamber (602) is connected to a liquid inlet (604), and a sealing mechanism (7) is fixed in the liquid inlet (604), and a vertical rod (605) is slidably connected to the sampling barrel (601), and piston heads (606) are evenly spaced on the vertical rod (605), and the piston heads (606) and the sample storage chambers (602) are distributed in a one-to-one correspondence, and the piston heads (606) and the sample storage chambers (602) are slidably connected, and a return spring (6061) is also fixed between the piston head (606) and the sample storage chamber (602),A curved surface block (607) is fixed to the upper end of the vertical rod (605), and the curved surface block (607) and the clamping block (505) are in a clamping connection. A top rod (608) is also fixed to the upper end of the curved surface block (607), and the top rod (608) is located directly below the contact sensor (502), and the height of the top rod (608) and the curved surface block (607) is equal to the distance between the clamping block (505) and the contact sensor (502).

2. A sampling and detection device for preparing collagen matrix according to claim 1, characterized in that: The sealing mechanism (7) comprises a partition (701), a sealing plug (702), a fixing rod (703), a fixing plate (704), a sliding rod (705) and a second spring (706); the partition (701) is fixed in the liquid inlet (604), and the partition (701) and the sealing plug (702) are nestedly connected.

3. A sampling and detection device for preparing collagen matrix according to claim 2, characterized in that: The sealing plug (702) is fixed to one end of the fixing rod (703), and the other end of the fixing rod (703) is fixed to the fixing plate (704). A sliding rod (705) is fixed to the fixing plate (704), and the sliding rod (705) is slidably connected to the partition plate (701).

4. A sampling and detection device for preparing collagen matrix according to claim 3, characterized in that: The fixing plate (704) and one end of the second spring (706) are fixed to each other, and the other end of the second spring (706) is fixed to the partition plate (701).

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