A flow cytometer

Abstract

The present invention provides a flow cytometer, which relates to the technical field of flow cytometers. It includes a main machine housing. A sampler housing is fixedly provided on the lower side of the right end of the main machine housing. The interior of the upper side of the right end of the main machine housing is in communication with the interior of the sampler housing. A sampling component is provided inside the sampler housing. A liquid path component and a power circuit component are provided inside the lower side of the main machine housing. The liquid path component and the power circuit component are distributed front and back. A stable optical platform component and a detection module component are provided inside the upper side of the main machine housing. The stable optical platform component and the detection module component are electrically connected. A sampling component is provided inside the upper side of the right end of the main machine housing. The sampling component is arranged corresponding to the sampling component. The liquid path component is in communication with the sampling component. The liquid path component is arranged corresponding to the stable optical platform component. The stable optical platform component has a stable anti-vibration function. When an external force is transmitted to the main machine housing, it can ensure that the laser shaping optical path in the stable optical platform component and the fluorescence spectral splitting path received by the detection module remain stable.

Description

Technical Field

[0001] The present invention relates to the technical field of flow cytometers, and specifically relates to a flow cytometer. Background Art

[0002] This flow cytometer is a high-end analytical instrument based on flow cytometry and spectral splitting technology. Its core feature is to analyze the fluorescence signals of cells or particles through full-spectrum detection technology. It can simultaneously capture the complete emission spectrum of fluorescent dyes, and combine algorithms to analyze overlapping spectra, significantly improving the multi-color detection ability and data accuracy. It breaks through the limitation of "one detector corresponding to one marker" in traditional flow cytometry, and uses a series of optical elements (prisms, gratings, combinations of various filters, etc.) to disperse the complete emission spectrum generated after the marker is excited onto a sensitive photodetector array to obtain the full-spectrum signal of the cells;

[0003] In the existing flow cytometers, the optical elements are prone to be interfered by vibrations and temperature in the external environment during operation, making it difficult to keep the light source path emitted by the optical elements stable. The spectral detector in the optical element has a reduced working efficiency at high temperatures, thus affecting the detection effect. Summary of the Invention

[0004] The present invention provides a flow cytometer to solve the above-mentioned technical problem that in the existing flow cytometers, the optical elements are prone to be interfered by vibrations and temperature in the external environment during operation, making it difficult to keep the light source path emitted by the optical elements stable. The spectral detector in the optical element has a reduced working efficiency at high temperatures, thus affecting the detection effect.

[0005] To solve the above technical problem, the present invention discloses a flow cytometer, which includes a main machine housing. An injector housing is fixedly provided on the lower right side of the main machine housing. The interior of the upper right side of the main machine housing is communicated with the interior of the injector housing. An injection assembly is provided inside the injector housing. A liquid path assembly and a power circuit assembly are provided inside the lower side of the main machine housing. The liquid path assembly and the power circuit assembly are distributed front and back. A stable optical platform assembly and a detection module assembly are provided inside the upper side of the main machine housing. The stable optical platform assembly and the detection module assembly are electrically connected. A sampling assembly is provided inside the upper right side of the main machine housing. The sampling assembly is correspondingly arranged with the injection assembly. The liquid path assembly is communicated with the sampling assembly. The liquid path assembly is correspondingly arranged with the stable optical platform assembly, and a heat dissipation assembly is provided inside the main machine housing.

[0006] Preferably, the sampling assembly includes a sampling chamber disposed inside the upper side of the right end of the main machine housing. On the front and rear sides of the sampling chamber, there are respectively a second pulley. The second pulley at the rear side is fixedly connected to the second motor, and the second motor is fixedly arranged in the sampling chamber. A first conveyor belt is connected between the second pulleys on the front and rear sides. On the upper side of the first conveyor belt, there is a connecting block which is slidably arranged on the sliding block in the front-rear direction. The sliding block is fixedly arranged in the sampling chamber. On the upper and lower sides of one end of the connecting block away from the first conveyor belt, there are respectively a third pulley. A second conveyor belt is connected between the third pulleys on the upper and lower sides. The front side of the second conveyor belt is fixedly connected to the mounting shell, and the mounting shell is connected to the sampling needle. The third pulley on the upper side is fixedly connected to the third motor, and the third motor is fixedly arranged on the connecting block.

[0007] Preferably, the upper end of the injector housing is provided with a flip cover. The injection assembly includes a first cavity disposed inside the injector housing. At the lower side of the first cavity, there is a fixed bottom plate. On the upper end of the bottom plate, there is a fixed first motor. The first motor is connected to the first pulley on the right side. The first pulley on the right side is connected to the first pulley on the left side through a third conveyor belt. The upper side of the third conveyor belt is connected to the positioning base shell which is slidably arranged on the upper end of the bottom plate in the left-right direction. The upper end of the positioning base shell is used for placing the sample tube rack, and the sample tube rack is correspondingly arranged under the sampling needle.

[0008] Preferably, the liquid path assembly includes a liquid path chamber disposed inside the lower side of the main machine housing. Inside the liquid path chamber, there are respectively a first peristaltic pump, a second peristaltic pump, a first diaphragm pump, a second diaphragm pump, a first filter, a second filter, a buffer pool, a plunger pump, a solenoid valve group and a three-way solenoid valve. At the lower left end of the main machine housing, there are a liquid inlet pipe fitting and a liquid outlet pipe fitting. The inlet of the liquid inlet pipe fitting is communicated with the sheath liquid container through a first liquid delivery pipe. The outlet of the liquid inlet pipe fitting is communicated with the inlet of the first diaphragm pump through a second liquid delivery pipe. The outlet of the first diaphragm pump is communicated with the inlet of the solenoid valve group through a third liquid delivery pipe. The first outlet of the solenoid valve group is communicated with the inlet of the first filter through a first connecting pipe. The second outlet of the solenoid valve group is communicated with the inlet of the second filter through a second connecting pipe. The outlets of the first filter and the second filter are respectively communicated with the inlet of the buffer pool through a liquid delivery pipe eleven. The first outlet of the buffer pool is communicated with the inlet of the first peristaltic pump through a fourth liquid delivery pipe. The outlet of the first peristaltic pump is communicated with a fifth liquid delivery pipe. The fifth liquid delivery pipe is communicated with the inlet of the plunger pump. The outlet of the plunger pump is communicated with the first port of the three-way solenoid valve through a sixth liquid delivery pipe. The second port of the three-way solenoid valve is communicated with the sampling needle through a seventh liquid delivery pipe. The third port of the three-way solenoid valve is communicated with the first inlet of the flow cell through an eighth liquid delivery pipe. The second outlet of the buffer pool is communicated with the inlet of the second peristaltic pump through a ninth liquid delivery pipe. The outlet of the second peristaltic pump is communicated with the second inlet of the flow cell through a tenth liquid delivery pipe. The outlet of the flow cell is communicated with the inlet of the waste liquid container through a twelfth liquid delivery pipe and the liquid outlet pipe fitting.

[0009] Preferably, a collection swab is provided in the sampling chamber. The collection swab is correspondingly arranged below the sampling needle. The collection swab is connected to the inlet of the diaphragm pump II through the liquid delivery pipe XIII. The outlet of the diaphragm pump II is connected to the second inlet of the waste liquid container through the liquid delivery pipe XIV and the liquid outlet fitting.

[0010] Preferably, the stable optical platform assembly includes a cavity II provided inside the upper side of the main machine housing. A partition is fixedly provided between the cavity II and the sampling chamber. The partition is provided with a mounting hole for the liquid delivery pipe X to pass through. On the right side of the upper end of the partition, there is a stable housing. Inside the stable housing, three lasers are arranged in the front-back direction. The three lasers are correspondingly arranged with three long focal length lenses one by one. The three long focal length lenses are correspondingly arranged with three dichroic mirrors one by one. The lasers, long focal length lenses and dichroic mirrors are arranged in sequence from left to right. A flow cell is correspondingly provided in front of the dichroic mirror. A rectifying block is provided between the flow cell and the frontmost dichroic mirror. The left side of the flow cell is correspondingly arranged with a side diffusion lens. The front side of the flow cell is correspondingly arranged with a front diffusion lens. The front diffusion lens is correspondingly connected to the front diffusion detector. The side diffusion lens is electrically connected to the detection module assembly respectively. A damping plate is provided at the lower end of the stable housing. At the lower end corners of the damping plate, a buffer column is respectively provided. The buffer column is fixedly connected to the partition.

[0011] Preferably, the heat dissipation assembly includes an air inlet I provided at the front end of the main machine housing. The air inlet I is correspondingly communicated with the heat dissipation housing. An air outlet I is provided at the upper side of the rear end of the main machine housing. The air outlet I is correspondingly arranged with the heat dissipation housing. An exhaust fan I is installed at the rear end of the heat dissipation housing. The heat dissipation housing is installed on the left side of the lower end of the partition. The front and rear ends of the heat dissipation housing are provided with heat dissipation holes. A number of heat dissipation plates are evenly arranged at intervals in the left-right direction in the heat dissipation holes.

[0012] Preferably, the power supply circuit assembly includes a board box. An exhaust fan II is installed at the rear end of the board box. An air outlet II is provided at the lower side of the rear end of the main machine housing. The air outlet II is correspondingly communicated with the exhaust fan II. A heat dissipation bottom plate is provided at the lower end of the board box. The air inlet provided at the left end of the main machine housing is communicated with the inside of the lower side of the housing.

[0013] Preferably, an exhaust fan III is installed at the rear end inside the main machine housing. The board box and the exhaust fan III are distributed left and right inside the main machine housing. An air outlet III is provided at the rear end of the main machine housing.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The stable optical platform assembly has a stable anti-vibration effect. When an external force is transmitted to the main machine housing, it can ensure that the light source path emitted in the stable optical platform assembly and the fluorescence spectroscopy path received by the detection module remain stable. The setting of the heat dissipation assembly is used to enable the spectral detector in the detection module assembly to work within the target temperature range, improving the working efficiency and operation stability of the spectral detector. Description of the Drawings

[0016] The accompanying drawings are used to provide a further understanding of the present invention, and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention, but do not constitute a limitation to the present invention. In the drawings:

[0017] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0018] Figure 2 is a schematic top view structure diagram of the present invention;

[0019] Figure 3 is a schematic front view structure diagram of the present invention;

[0020] Figure 4 is a schematic diagram of the internal structure of the present invention;

[0021] Figure 5 is a schematic diagram of the structure of the stable optical platform assembly of the present invention;

[0022] Figure 6 is a schematic diagram of the installation position of the board box of the present invention;

[0023] Figure 7 is a schematic diagram of the installation positions of exhaust fan 1, exhaust fan 2, and exhaust fan 3 of the present invention;

[0024] Figure 8 is a schematic diagram of the board box structure of the present invention.

[0025] In the figure: 1, sampling chamber; 101, conveyor belt 1; 102, mounting block; 103, sliding block; 104, conveyor belt 2; 105, mounting shell; 106, sampling needle; 2, bottom plate; 201, motor 1; 202, conveyor belt 3; 203, positioning base shell; 3, liquid path chamber; 301, peristaltic pump 1; 302, diaphragm pump 1; 303, liquid inlet pipe fitting; 304, filter 1; 305, buffer pool; 306, plunger pump; 307, solenoid valve group; 308, flow cell; 309, liquid outlet pipe fitting; 310, filter 2; 311, three-way solenoid valve; 312, peristaltic pump 2; 313, diaphragm pump 2; 314, collection swab; 315, liquid delivery pipe 10; 4, stable shell; 401, laser; 402, dichroic mirror; 403, rectifier block; 404, long-focus lens; 405, side-diffusing lens; 406, buffer column; 407, front-diffusing lens; 408, front-diffusing detector; 5, spectral detector; 6, heat dissipation shell; 7, main machine housing; 701, air inlet 2; 8, sampler housing; 801, flip cover; 9, sample tube rack; 10, vibration damping plate; 11, board box; 12, heat dissipation bottom plate; 13, exhaust fan 1; 14, exhaust fan 2; 15, exhaust fan 3. Detailed implementation manners

[0026] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only for the purpose of illustrating and explaining the present invention, and are not used to limit the present invention.

[0027] In addition, in the present invention, descriptions such as "first" and "second" are only for descriptive purposes, and do not particularly refer to the meaning of order or sequence, nor are they used to limit the present invention. They are merely used to distinguish components or operations described with the same technical terms, and should not be construed as indicating or implying their relative importance or implicitly indicating the quantity of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include at least one of such features. In addition, the technical solutions and technical features between various embodiments may be combined with each other, but it must be based on the ability of those of ordinary skill in the art to implement. When the combination of technical solutions results in contradictions or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0028] The present invention provides the following embodiments.

[0029] Embodiment 1

[0030] The embodiment of the present invention provides a flow cytometer, as Figures 1 - 5 shown, which includes a main machine housing 7. A sampler housing 8 is fixedly provided on the lower right side of the main machine housing 7. The inside of the upper right side of the main machine housing 7 is communicated with the inside of the sampler housing 8. A sampling assembly is provided inside the sampler housing 8. A liquid path assembly and a power circuit assembly are provided inside the lower side of the main machine housing 7. The liquid path assembly and the power circuit assembly are distributed front and back. A stable optical platform assembly and a detection module assembly are provided inside the upper side of the main machine housing 7. The stable optical platform assembly and the detection module assembly are electrically connected. A sampling assembly is provided inside the upper right side of the main machine housing 7. The sampling assembly is correspondingly arranged with the sampling assembly. The liquid path assembly is communicated with the sampling assembly. The liquid path assembly is correspondingly arranged with the stable optical platform assembly, and a heat dissipation assembly is provided inside the main machine housing 7.

[0031] The beneficial effects of the above technical solutions are:

[0032] The sample to be detected can be placed on the sample injection assembly and sent to the lower right side of the main machine housing 7, so that the sample to be detected is located below the sampling assembly. Then, the sampling assembly samples the sample to be detected. The liquid path assembly is used to send the sample to be detected and the sheath fluid to the detection chamber for mixing. The sheath fluid enables the sample to be detected to pass through the detection chamber in a straight line. The laser light source emitted by the stable optical platform assembly hits the sample to be detected, causing the sample to be detected to generate a fluorescence signal. The detection module assembly converts the collected fluorescence signal into an electrical signal through a photomultiplier tube and amplifies and processes the electrical signal. The amplified electrical signal is sent to the computer system for data analysis to obtain spectral characteristics. The liquid path assembly is arranged inside the lower side of the main machine housing 7, and the stable optical platform assembly and the detection module assembly are arranged inside the upper side of the main machine housing 7, separating the liquid path assembly from the stable optical platform assembly and the detection module assembly, reducing the mutual influence between the liquid path and the optical path, and also facilitating the separate maintenance and replacement of the liquid path assembly, the stable optical platform assembly, and the detection module assembly. The stable optical platform assembly has a stable anti-vibration function. When an external force is transmitted to the main machine housing 7, it can ensure that the light source path emitted by the stable optical platform assembly remains stable. The heat dissipation assembly is provided to enable the spectral detector 5 in the detection module assembly to work within the target temperature range, improving the working efficiency and operation stability of the spectral detector 5, and solving the technical problems that the optical elements in the existing flow cytometer are prone to be interfered by the vibration and temperature of the external environment during operation, making it difficult to maintain the stability of the light source path emitted by the optical elements, and the working efficiency of the spectral detector in the optical elements decreases at high temperatures, thus affecting the stability of the detection effect. The sample injection assembly, the liquid path assembly, the stable optical platform assembly, the detection module assembly, the sampling assembly, and the heat dissipation assembly can be installed as independent modules in different areas of the main machine housing 7 respectively, making the whole flow cytometer of the present invention a modular design, facilitating the disassembly and assembly of the sample injection assembly, the liquid path assembly, the stable optical platform assembly, the detection module assembly, the sampling assembly, and the heat dissipation assembly separately, and further facilitating the manufacture and maintenance of the overall structure of the flow cytometer.

[0033] Embodiment 2

[0034] Based on Embodiment 1, as Figures 1 - 5As shown in the figure, the sampling component includes a sampling chamber 1 arranged inside the upper side of the right end of the main machine housing 7. Pulley two is provided on each of the front and rear sides of the sampling chamber 1. The pulley two at the rear side is fixedly connected to the second motor. The second motor is fixedly arranged in the sampling chamber 1. A first conveyor belt 101 is connected between the pulley two on the front and rear sides. The upper side of the first conveyor belt 101 is connected with a mounting block 102. The mounting block 102 is arranged to slide in the front-rear direction on a sliding block 103. The sliding block 103 is fixedly arranged in the sampling chamber 1. Pulley three is provided on each of the upper and lower sides of the end of the mounting block 102 away from the first conveyor belt 101. A second conveyor belt 104 is connected between the pulley three on the upper and lower sides. The front side of the second conveyor belt 104 is fixedly connected to a mounting shell 105. The mounting shell 105 is connected to a sampling needle 106. The pulley three on the upper side is fixedly connected to the third motor. The third motor is fixedly arranged on the mounting block 102;

[0035] A flip cover 801 is provided at the upper end of the injector housing 8. The injection component includes a first cavity arranged inside the injector housing 8. A bottom plate 2 is fixedly provided at the lower side of the first cavity. A first motor 201 is fixedly provided at the upper end of the bottom plate 2. The first motor 201 is connected to the pulley one on the right side. The pulley one on the right side is connected to the pulley one on the left side through a third conveyor belt 202. The upper side of the third conveyor belt 202 is connected to a positioning base shell 203. The positioning base shell 203 is arranged to slide in the left-right direction on the upper end of the bottom plate 2. The upper end of the positioning base shell 203 is used for placing a sample tube rack 9. The sample tube rack 9 is correspondingly arranged under the sampling needle 106.

[0036] The beneficial effects of the above technical solutions are as follows:

[0037] When the second motor works, it can drive the pulley two at the rear side to rotate. The pulley two at the rear side drives the pulley two at the front side to rotate through the first conveyor belt 101. When the first conveyor belt 101 moves along with the pulley two on the front and rear sides, the mounting block 102 can move back and forth along with the first conveyor belt 101. The mounting block 102 drives the pulley three on the upper and lower sides, the third motor, the second conveyor belt 104, the mounting shell 105 and the sampling needle 106 to move back and forth. When the third motor works, it drives the pulley three on the upper side to rotate. The pulley three on the upper side drives the pulley three on the lower side to rotate through the second conveyor belt 104. When the second conveyor belt 104 moves along with the pulley three on the upper and lower sides, the mounting shell 105 and the sampling needle 106 can move up and down, thus achieving the purpose of the sampling needle 106 moving back and forth and up and down;

[0038] When the first motor 201 operates, it can drive the first pulley on the right side to rotate. The first pulley on the right side drives the first pulley on the left side to rotate through the third conveyor belt 202. When the third conveyor belt 202 rotates with the first pulleys on both the left and right sides, it can move left and right, thereby driving the positioning base shell 203 to move left and right. The positioning base shell 203 drives the sample tube rack 9 to move left and right. When detecting a sample, open the flip cover 801, place the sample tube rack 9 containing the sample on the positioning base shell 203, and then control the first motor 201 to operate, so that the sample tube rack 9 containing the sample moves to the corresponding position below the sampling needle 106. Since the sampling needle 106 can move in the front-back and up-down directions and the sample tube rack 9 containing the sample can move left and right, by controlling the first motor 201, the second motor, and the third motor to operate, the sampling needle 106 can sample the sample at any position on the sample tube rack 9.

[0039] Embodiment 3

[0040] On the basis of Embodiment 2, as Figures 1 - 5 shown, the liquid path assembly includes a liquid path chamber 3 provided inside the lower side of the main machine housing 7. Inside the liquid path chamber 3, there are respectively provided a peristaltic pump 301, a peristaltic pump 312, a diaphragm pump 302, a diaphragm pump 313, a filter 304, a filter 310, a buffer tank 305, a plunger pump 306, a solenoid valve group 307, and a three-way solenoid valve 311. At the left end of the lower side of the main machine housing 7, there are provided a liquid inlet pipe fitting 303 and a liquid outlet pipe fitting 309. The inlet of the liquid inlet pipe fitting 303 is connected to the sheath liquid container through a first liquid delivery pipe. The outlet of the liquid inlet pipe fitting 303 is connected to the inlet of the diaphragm pump 302 through a second liquid delivery pipe. The outlet of the diaphragm pump 302 is connected to the inlet of the solenoid valve group 307 through a third liquid delivery pipe. The first outlet of the solenoid valve group 307 is connected to the inlet of the filter 304 through a first connecting pipe. The second outlet of the solenoid valve group 307 is connected to the inlet of the filter 310 through a second connecting pipe. The outlets of the filter 304 and the filter 310 are respectively connected to the inlet of the buffer tank 305 through an eleventh liquid delivery pipe. The first outlet of the buffer tank 305 is connected to the inlet of the peristaltic pump 301 through a fourth liquid delivery pipe. The outlet of the peristaltic pump 301 is connected to a fifth liquid delivery pipe. The fifth liquid delivery pipe is connected to the inlet of the plunger pump 306. The outlet of the plunger pump 306 is connected to the first port of the three-way solenoid valve 311 through a sixth liquid delivery pipe. The second port of the three-way solenoid valve 311 is connected to the sampling needle 106 through a seventh liquid delivery pipe. The third port of the three-way solenoid valve 311 is connected to the first inlet of the flow cell 308 through an eighth liquid delivery pipe. The second outlet of the buffer tank 305 is connected to the inlet of the peristaltic pump 312 through a ninth liquid delivery pipe. The outlet of the peristaltic pump 312 is connected to the second inlet of the flow cell 308 through a tenth liquid delivery pipe 315. The outlet of the flow cell 308 is connected to the inlet of the waste liquid container through a twelfth liquid delivery pipe and the liquid outlet pipe fitting 309;

[0041] A collecting swab 314 is provided in the sampling chamber 1, and the collecting swab 314 is correspondingly arranged on the lower side of the sampling needle 106. The collecting swab 314 is connected to the inlet of the diaphragm pump 2 313 through the liquid delivery tube 13, and the outlet of the diaphragm pump 2 313 is connected to the inlet 2 of the waste liquid container through the liquid delivery tube 14 and the liquid outlet pipe 309.

[0042] The beneficial effects of the above technical solution are:

[0043] The plurality of liquid delivery pipes and connecting pipes in the sheath liquid container and the waste liquid container liquid path assembly arranged outside the main machine housing 7 are not Figures 1 - 5 As shown, when the diaphragm pump 1 302 is working, the sheath liquid in the sheath liquid container is sent to the buffer tank 305 through the liquid inlet pipe 303, the liquid delivery pipe 1, the liquid delivery pipe 2, the liquid delivery pipe 3, the electromagnetic valve group 307, the connecting pipe 1, the filter 1 304 and the liquid delivery pipe 11. The filter 1 304 sterilizes the sheath liquid, and the buffer tank 305 temporarily stores the sheath liquid to ensure a stable supply of the sheath liquid. The sheath liquid in the buffer tank 305 enters the peristaltic pump 1 301 through the liquid delivery pipe 4 and enters the peristaltic pump 2 312 through the liquid delivery pipe 9. The peristaltic pump 2 312 sends the sheath liquid to the peristaltic pump 1 301. The liquid is directly sent into the flow chamber 308 to provide the sample with the sheath liquid. When the sample needs to be sampled, the peristaltic pump 1 301 and the peristaltic pump 2 312 do not work, the port 3 of the three-way solenoid valve 311 is disconnected, and the plunger pump 306 works to suck the sample out of the sampling needle 106, so that the sample enters the liquid delivery tube 6 through the liquid delivery tube 7, the port 2 and the port 1, and then the port 2 is closed and the port 3 is opened. Under the power of the peristaltic pump 1 301 and the plunger pump 306, the sample and the sheath liquid are sent into the flow chamber 308 through the liquid delivery tube 8 to mix with the sheath liquid in the flow chamber 308;

[0044] During the sample detection process, under the power of peristaltic pump II 312, peristaltic pump I 301, and plunger pump 306, the sheath fluid and the sample in the flow cell 308 flow and enter the waste liquid container through delivery pipe XII. When cleaning the sampling needle 106 after the sample detection is completed, port three of the three-way solenoid valve 311 is disconnected, the plunger pump 306 does not work, and when the peristaltic pump I 301 works, it sends the sheath fluid into the sampling needle 106 to rinse the sampling needle 106. The waste liquid after rinsing falls into the collection swab, and the waste liquid in the collection swab enters the diaphragm pump II 313 through delivery pipe IX. The diaphragm pump II 313 sends the waste liquid into the waste liquid container through delivery pipe XIV. When cleaning the liquid path assembly, delivery pipe I is connected to the cleaning liquid container to make the flow path of the cleaning liquid the same as the flow path of the sheath fluid in the above process to clean the liquid path assembly. The settings of filter I 304 and filter II 310 are exactly the same, and one of the filters is used as a spare. Specifically, when the filtering effect of filter I 304 is affected and needs to be maintained and replaced, by controlling the solenoid valve group 307, inlet one of the solenoid valve group 307 is closed and inlet two is opened, so that the sheath fluid flows from passing through filter I 304 to passing through filter II 310. When outlet one of the solenoid valve group 307 is closed, outlet two is opened, and when outlet one is opened, outlet two is closed.

[0045] Example 4

[0046] Based on Example 3, as Figures 1 - 6 shown, the stable optical platform assembly includes cavity II provided inside the upper side of the main machine housing 7. A partition is fixedly provided between cavity II and the sampling cavity 1. The partition is provided with a mounting hole through which delivery pipe X 315 passes. A stable housing 4 is provided on the upper right side of the upper end of the partition. Inside the stable housing 4, three lasers 401 are provided along the front-rear direction. The three lasers 401 are arranged in one-to-one correspondence with three long-focus lenses 404, and the three long-focus lenses 404 are arranged in one-to-one correspondence with three dichroic mirrors 402. The lasers 401, long-focus lenses 404, and dichroic mirrors 402 are arranged in sequence from left to right. A flow cell 308 is correspondingly provided on the front side of the dichroic mirror 402. A rectifying block 403 is provided between the flow cell 308 and the frontmost dichroic mirror 402. The left side of the flow cell 308 is correspondingly provided with a side-diffusing lens 405, and the front side of the flow cell 308 is correspondingly provided with a front-diffusing lens 407. The front-diffusing lens 407 is correspondingly connected to the front-diffusing detector 408. The side-diffusing lens 405 is electrically connected to the detection module assembly respectively. A damping plate 10 is provided at the lower end of the stable housing 4. Buffer columns 406 are respectively provided at the lower end corners of the damping plate 10, and the buffer columns 406 are fixedly connected to the partition.

[0047] The detection module assembly includes three spectral detectors 5, and the three spectral detectors 5 are respectively electrically connected to the side-diffusing lens 405. The three spectral detectors 5 are installed on the upper left side of the partition.

[0048] The heat dissipation component includes an air inlet one provided at the front end of the main machine housing 7. The air inlet one is correspondingly communicated with the heat dissipation housing 6. An air outlet one is provided on the upper side of the rear end of the main machine housing 7. The air outlet one is correspondingly arranged with the heat dissipation housing 6. An exhaust fan one 13 is installed at the rear end of the heat dissipation housing 6. The heat dissipation housing 6 is installed at the lower left end of the partition board. Heat dissipation holes are provided through the front and rear ends of the heat dissipation housing. A plurality of heat dissipation plates are evenly arranged at intervals in the left-right direction in the heat dissipation holes.

[0049] The beneficial effects of the above technical solution are as follows:

[0050] Three lasers 401 are used to emit light of different wavelengths. The telephoto lens 404 is used to focus the light emitted by its corresponding laser 401. The dichroic mirror 402 is used to combine the light emitted by its corresponding laser 401. The three combined beams of light pass through the rectifying block 403. Cylindrical lenses are respectively provided on the left and right sides of the rectifying block 403, so as to focus the three beams of light, and the focused light path hits the sample in the flow cell 308. The generated side-scattered light is collected by the side-scattering lens 405. The three spectral detectors 5 have different models and are correspondingly adapted to the three lasers 401 one by one, and are used to process the side-scattered light generated when the light beam emitted by the laser 401 corresponding to the spectral detector 5 hits the sample. The side-scattered light can be used to detect the internal structure attributes of cells. The forward-scattered light generated when hitting the sample passes through the forward-scattering lens 407 and enters the forward-scattering detector. The forward-scattered light is used as a threshold to exclude debris in the sample and small particles in the sheath fluid, so as to avoid interference with the cells in the sample to be measured. The setting of the buffer column 406 enables the stable housing 4 to have a shock absorption and buffering effect. When an external force is transmitted to the main machine housing 7, the laser emitted by the three lasers 401 and the laser path are kept stable, avoiding the laser path from shifting and being unable to hit the sample, and ensuring that the light source path emitted by the stable optical platform component remains stable. Furthermore, the acquisition of the optical signal is kept stable, improving the detection effect;

[0051] The spectral detector 5 collects signal data of the side-scattered light and analyzes it to obtain a fluorescence signal. The forward scatter detector collects signal data of the forward-scattered light and analyzes it to obtain a fluorescence intensity threshold range. The processed fluorescence intensity is compared with the fluorescence intensity threshold range, and the fluorescence intensity exceeding the threshold range is removed until the remaining non-filtered fluorescence intensity conforms to the fluorescence intensity threshold range. The remaining fluorescence signals are used as an array. The obtained fluorescence signal array is converted into an electrical signal by a photomultiplier tube and the electrical signal is amplified. The amplified electrical signal is sent into a computer system for data analysis to obtain spectral characteristics (the three lasers 401, the photomultiplier tube, the forward scatter detector, and the spectral detector 5 all adopt existing products, and the fluorescence intensity is obtained by collecting signal data of light by the detector and analyzing it. Converting the collected fluorescence signal into an electrical signal by a photomultiplier tube and amplifying the electrical signal, and sending the amplified electrical signal into a computer system for data analysis adopt existing technologies, which will not be elaborated in the present invention);

[0052] The setting of the first air inlet enables the outside air to enter the interior of the main machine housing 7, pass through the heat dissipation housing 6 and then be discharged through the first air outlet. A number of heat dissipation plates are evenly arranged at intervals in the left-right direction in the heat dissipation holes. A protective fence is also provided at the front end of the heat dissipation holes. The adjacent heat dissipation plates form a heat dissipation channel, enabling the air to pass through a number of heat dissipation channels and finally flow out through the air outlet. The contact end between the partition plate and the three spectral detectors 5 is fixedly connected to the upper end of the heat dissipation housing 6. The contact end between the partition plate and the three spectral detectors 5 (the partition plate is in direct contact with the PCB circuit board of the spectral detector 5) and the heat dissipation housing 6 are made of the same material, both of which are heat-conducting materials, so as to improve the heat dissipation effect on the three spectral detectors 5;

[0053] Optionally, the exhaust fan 13 in the air outlet can be automatically controlled to work through the following control method. Specifically, a first temperature sensor is set at the first air inlet to detect the temperature of the air flowing in the first air inlet; a number of second temperature sensors are installed in the heat dissipation housing 6, and the number of second temperature sensors are respectively arranged between the heat dissipation channels formed by the adjacent heat dissipation plates to detect the temperatures in different heat dissipation channels; a number of wind speed sensors are installed in the heat dissipation housing 6, and the number of wind speed sensors are respectively arranged between the heat dissipation channels formed by the adjacent heat dissipation plates to detect the wind speeds in different heat dissipation channels; a controller is set, and the controller is electrically connected to the first temperature sensor, the number of second temperature sensors, the number of wind speed sensors, and a frequency converter. The frequency converter is electrically connected to the exhaust fan. The controller calculates the theoretical wind speed required for the heat dissipation channel with the highest temperature per unit time to reach the target temperature according to the detection value of the first temperature sensor, the detection values of the number of second temperature sensors, and formula (1);

[0054] ; (1)

[0055] The theoretical wind speed required for the heat dissipation channel with the highest temperature within a unit time to reach the target temperature The maximum detected value among several temperature sensors The detected value of Temperature Sensor 1 The air density The dynamic viscosity of the air in the heat dissipation channel The gap between adjacent heat dissipation plates The Prandtl number, with a value of 0.5 The thickness of the partition The thermal conductivity of the partition The thickness of the upper end of the heat dissipation shell 6 The thermal conductivity of the heat dissipation shell 6 The target temperature of the heat dissipation channel The thermal conductivity of the air

[0056] Formula (1) is derived from When the temperature value of the heat dissipation channel with the highest temperature is greater than the target temperature, the controller automatically increases the speed of the exhaust fan through the frequency converter to increase the wind speed in the heat dissipation channel. The controller compares the minimum wind speed detected by several wind speed sensors in the heat dissipation duct with the theoretical wind speed required for the heat dissipation channel with the highest temperature within a unit time calculated above until the minimum wind speed detected by several wind speed sensors in the heat dissipation duct reaches the theoretical wind speed required for the heat dissipation channel with the highest temperature within a unit time calculated above. At this time, the controller no longer adjusts the speed of the exhaust fan through the frequency converter, so that the speed of the exhaust fan remains at the current speed, thus ensuring the heat dissipation effect of the three spectral detectors 5 and enabling the spectral detector 5 to operate stably. The unit time is 1 s, so that the temperature of the heat dissipation duct can be adjusted in time, avoiding the too long cooling time of the heat dissipation duct and affecting the operation of the spectral detector 5. When the temperature value of the heat dissipation channel with the highest temperature is less than the target temperature, that is, when the theoretical wind speed required for the heat dissipation channel with the highest temperature within a unit time calculated above is negative, the controller reduces the speed of the exhaust fan through the frequency converter so that it works in a low energy consumption state until the temperature value of the heat dissipation channel with the highest temperature is greater than the target temperature. Then repeat the above steps of the controller automatically increasing the speed of the exhaust fan through the frequency converter to increase the wind speed in the heat dissipation channel. By automatically adjusting the speed of the exhaust fan, the spectral detector 5 is automatically cooled, ensuring that the spectral detector 5 can always operate stably and without manual operation, saving time and effort.

[0057] Example 5

[0058] On the basis of Example 3, as Figures 6 - 8As shown in the figure, the power supply circuit assembly includes a board box 11, in which electrical components are installed. An exhaust fan II 14 is installed at the rear end of the board box 11. There is an air outlet II at the lower side of the rear end of the main machine housing 7, and the air outlet II is correspondingly communicated with the exhaust fan II 14. A heat dissipation bottom plate 12 is provided at the lower end of the board box 11. The air inlet II 701 provided at the left end of the main machine housing 7 is communicated with the inside of the lower side of the main machine housing 7;

[0059] An exhaust fan III 15 is installed at the inner rear end of the main machine housing 7. The board box 11 and the exhaust fan III 15 are distributed left and right inside the main machine housing 7. There is an air outlet III at the rear end of the main machine housing 7.

[0060] The beneficial effects of the above technical solution are as follows:

[0061] The board box 11 and the electrical components installed therein are equivalent to the existing electric control cabinet, which will not be elaborated in this invention. When the exhaust fan II 14 works, it will cause the outside air to enter the inside of the main machine housing 7 through the air inlet II 701, and then be discharged through the air outlet II, which can dissipate heat from the electrical components in the board box 11. When the exhaust fan III 15 works, it will cause the outside air to enter the inside of the main machine housing 7 through the air inlet II 701, and then be discharged through the air outlet III, dissipating heat from the inside of the main machine housing 7, such as the liquid path assembly, improving the working stability of the flow cytometer of this invention. Filters can be provided at the air inlet I and the air inlet II 701 to prevent the dust carried by the air from affecting the components in the flow cytometer.

[0062] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention also intends to include these changes and modifications.

Claims

1. A flow cytometer, characterized in that: It includes a main machine housing (7). At the lower right side of the main machine housing (7), a sampler housing (8) is fixedly provided. The interior of the upper right side of the main machine housing (7) is in communication with the interior of the sampler housing (8). A sampling component is provided inside the sampler housing (8). Inside the lower side of the main machine housing (7), a liquid path component and a power circuit component are provided, and the liquid path component and the power circuit component are distributed front and back. Inside the upper side of the main machine housing (7), a stable optical platform component and a detection module component are provided, and the stable optical platform component and the detection module component are electrically connected. Inside the upper right side of the main machine housing (7), a sampling component is provided, and the sampling component is arranged corresponding to the sampling component. The liquid path component is in communication with the sampling component, and the liquid path component and the stable optical platform component are arranged corresponding to each other. Moreover, a heat dissipation component is provided inside the main machine housing (7); wherein, the stable optical platform component includes a cavity two provided inside the upper side of the main machine housing (7). A partition is fixedly provided between the cavity two and the sampling cavity (1). The partition is provided with a mounting hole through which a liquid delivery pipe ten (315) passes. At the right side of the upper end of the partition, a stable housing (4) is provided. Inside the stable housing (4), three lasers (401) are provided along the front-back direction. The three lasers (401) and three long focal length lenses (404) are arranged in one-to-one correspondence. The three long focal length lenses (404) and three dichroic mirrors (402) are arranged in one-to-one correspondence. The lasers (401), the long focal length lenses (404), and the dichroic mirrors (402) are arranged in sequence from left to right. A flow cell (308) is correspondingly provided at the front side of the dichroic mirror (402). A rectifying block (403) is provided between the flow cell (308) and the frontmost dichroic mirror (402). The left side of the flow cell (308) is arranged corresponding to a side diffusion lens (405). The front side of the flow cell (308) is arranged corresponding to a front diffusion lens (407). The front diffusion lens (407) is correspondingly connected to a front diffusion detector (408). The side diffusion lens (405) is electrically connected to the detection module component respectively. At the lower end of the stable housing (4), a vibration damping plate (10) is provided. At the lower end corners of the vibration damping plate (10), a buffer column (406) is provided respectively, and the buffer column (406) is fixedly connected to the partition.

2. A flow cytometer according to claim 1, characterized in that: The sampling component includes a sampling chamber (1) arranged inside the upper side of the right end of the main machine housing (7). There is a second pulley on each of the front and rear sides of the sampling chamber (1). The second pulley at the rear is fixedly connected to the second motor. The second motor is fixedly arranged in the sampling chamber (1). A first conveyor belt (101) is connected between the second pulleys on the front and rear sides. An installation block (102) is connected to the upper side of the first conveyor belt (101). The installation block (102) is slidably arranged in the sliding block (103) in the front-rear direction. The sliding block (103) is fixedly arranged in the sampling chamber (1). There is a third pulley on each of the upper and lower sides of the end of the installation block (102) away from the first conveyor belt (101). A second conveyor belt (104) is connected between the third pulleys on the upper and lower sides. The front side of the second conveyor belt (104) is fixedly connected to the installation shell (105). The installation shell (105) is connected to the sampling needle (106). The third pulley on the upper side is fixedly connected to the third motor. The third motor is fixedly arranged on the installation block (102).

3. A flow cytometer according to claim 2, characterized in that: A flip cover (801) is provided at the upper end of the injector housing (8). The injection component includes a first cavity arranged inside the injector housing (8). A bottom plate (2) is fixedly provided at the lower side of the first cavity. A first motor (201) is fixedly provided at the upper end of the bottom plate (2). The first motor (201) is connected to the first pulley on the right side. The first pulley on the right side is connected to the first pulley on the left side through a third conveyor belt (202). The upper side of the third conveyor belt (202) is connected to the positioning base shell (203). The positioning base shell (203) is slidably arranged at the upper end of the bottom plate (2) in the left-right direction. The upper end of the positioning base shell (203) is used to place the sample tube rack (9). The sample tube rack (9) is correspondingly arranged under the sampling needle (106).

4. A flow cytometer according to claim 2, characterized in that: The liquid path assembly includes a liquid path chamber (3) arranged inside the lower side of the main machine housing (7). Inside the liquid path chamber (3), there are respectively a peristaltic pump one (301), a peristaltic pump two (312), a diaphragm pump one (302), a diaphragm pump two (313), a filter one (304), a filter two (310), a buffer pool (305), a plunger pump (306), a solenoid valve group (307) and a three-way solenoid valve (311). At the left end of the lower side of the main machine housing (7), there are a liquid inlet pipe fitting (303) and a liquid outlet pipe fitting (309). The inlet of the liquid inlet pipe fitting (303) is connected to the sheath liquid container through a first liquid delivery pipe, the outlet of the liquid inlet pipe fitting (303) is connected to the inlet of the diaphragm pump one (302) through a second liquid delivery pipe, the outlet of the diaphragm pump one (302) is connected to the inlet of the solenoid valve group (307) through a third liquid delivery pipe, the first outlet of the solenoid valve group (307) is connected to the inlet of the filter one (304) through a first connecting pipe, the second outlet of the solenoid valve group (307) is connected to the inlet of the filter two (310) through a second connecting pipe, the outlets of the filter one (304) and the filter two (310) are respectively connected to the inlet of the buffer pool (305) through an eleventh liquid delivery pipe, the first outlet of the buffer pool (305) is connected to the inlet of the peristaltic pump one (301) through a fourth liquid delivery pipe, the outlet of the peristaltic pump one (301) is connected to a fifth liquid delivery pipe, the fifth liquid delivery pipe is connected to the inlet of the plunger pump (306), the outlet of the plunger pump (306) is connected to the first port of the three-way solenoid valve (311) through a sixth liquid delivery pipe, the second port of the three-way solenoid valve (311) is connected to the sampling needle (106) through a seventh liquid delivery pipe, the third port of the three-way solenoid valve (311) is connected to the first inlet of the flow cell (308) through an eighth liquid delivery pipe, the second outlet of the buffer pool (305) is connected to the inlet of the peristaltic pump two (312) through a ninth liquid delivery pipe, the outlet of the peristaltic pump two (312) is connected to the second inlet of the flow cell (308) through a tenth liquid delivery pipe (315), and the outlet of the flow cell (308) is connected to the inlet of the waste liquid container through a twelfth liquid delivery pipe and the liquid outlet pipe fitting (309).

5. A flow cytometer according to claim 4, characterized in that: A collection swab (314) is arranged in the sampling chamber (1). The collection swab (314) is correspondingly arranged below the sampling needle (106). The collection swab (314) is connected to the inlet of the diaphragm pump two (313) through a thirteenth liquid delivery pipe, and the outlet of the diaphragm pump two (313) is connected to the second inlet of the waste liquid container through a fourteenth liquid delivery pipe and the liquid outlet pipe fitting (309).

6. A flow cytometer according to claim 1, characterized in that: The detection module assembly includes three spectral detectors (5). The three spectral detectors (5) are respectively electrically connected to the side-dispersion lens (405). The three spectral detectors (5) are installed on the upper left side of the partition board.

7. A flow cytometer according to claim 1, characterized in that: The heat dissipation component includes that there is an air inlet one at the front end of the main machine housing (7), the air inlet one is correspondingly communicated with the heat dissipation housing (6), there is an air outlet one at the upper side of the rear end of the main machine housing (7), the air outlet one is correspondingly arranged with the heat dissipation housing (6), an exhaust fan one (13) is installed at the rear end of the heat dissipation housing (6), the heat dissipation housing (6) is installed at the lower left end of the partition board, heat dissipation holes are provided through the front and rear ends of the heat dissipation housing, and a plurality of heat dissipation plates are evenly arranged at intervals in the left - right direction in the heat dissipation holes.

8. A flow cytometer according to claim 1, wherein: The power circuit component includes a board box (11), an exhaust fan two (14) is installed at the rear end of the board box (11), there is an air outlet two at the lower side of the rear end of the main machine housing (7), the air outlet two is correspondingly communicated with the exhaust fan two (14), a heat dissipation bottom plate (12) is provided at the lower end of the board box (11), and the air inlet two (701) provided at the left end of the main machine housing (7) is communicated with the inside of the lower side of the main machine housing (7).

9. A flow cytometer according to claim 8, characterized in that: An exhaust fan three (15) is installed at the inner rear end of the main machine housing (7), the board box (11) and the exhaust fan three (15) are distributed left and right inside the main machine housing (7), and there is an air outlet three at the rear end of the main machine housing (7).

Images