Thin-layer chromatography detection method for herba epimedii in herba cistanche and herba epimedii black tea
Through the improved thin-layer chromatography detection method, the epimedium in Cistanche Epimedium black tea is tested using a specific proportion of the expansion agent and color developer, which solves the problem of inaccurate detection in the existing technology and achieves efficient and accurate quality control.
Patent Information
- Application Number
- CN202411977986.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-04-08
AI Technical Summary
The prior art lacks a method that can quickly and accurately detect epimedium in Cistanche Epimedium black tea, resulting in the inability to effectively control product quality.
An improved thin-layer chromatography detection method was adopted to prepare the test sample solution, the icariin control sample solution and the icarime control medicinal material solution, and the color development treatment was performed using a specific proportion of the expander and color development agent. After the color development treatment, the color development treatment was examined under the ultraviolet lamp.
It realizes efficient separation and accurate detection of epimedium in Cistanche Epimedium Black Tea, improves the accuracy and comprehensiveness of quality control, and has the advantages of high sensitivity, easy operation, low cost and short detection time.
Smart Images
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Abstract
Description
Technical Field
[0001] The present application relates to the technical field of food detection, and in particular to a thin layer chromatography detection method of epimedium in Cistanche deserticola and epimedium black tea. Background Art
[0002] Cistanche Epimedium Black Tea is a health food with the function of relieving physical fatigue, which is made of Cistanche, jujube, epimedium, Acanthopanax, black tea, dextrin and other main raw and auxiliary materials, using Chinese medicine water extraction technology combined with modern technology. Its characteristic ingredients are total saponins, tea polyphenols, icariin and so on. Therefore, epimedium is one of the important components of this product, which is closely related to the functions and indications of Cistanche Epimedium Black Tea. Therefore, it is necessary to establish a qualitative method of epimedium as a quality control indicator of Cistanche Epimedium Black Tea.
[0003] However, there is currently a lack of methods that can quickly identify and specifically detect epimedium in Cistanche and Epimedium black tea, and it is impossible to accurately and comprehensively determine or control the quality of Cistanche and Epimedium black tea. In addition, some methods use thin layer chromatography to detect other preparations containing epimedium or epimedium alone, but these methods generally have problems such as poor spot color development, unclear spots, and severe tailing, and poor detection accuracy. Summary of the invention
[0004] Based on this, the present application provides a thin layer chromatography detection method for Epimedium in Cistanche Epimedium black tea with good separation effect and high detection accuracy.
[0005] The specific technical solutions are as follows:
[0006] A thin layer chromatography detection method for epimedium in Cistanche deserticola epimedium black tea comprises the following steps:
[0007] Prepare the test solution of Cistanche deserticola and Epimedium black tea, the icariin reference solution and the Epimedium reference medicinal material solution;
[0008] The test solution of the Cistanche deserticola and Epimedium black tea to be tested, the icariin reference solution and the Epimedium reference medicinal material solution are spotted on the same silica gel thin layer plate, developed with a developing agent, taken out, dried and then subjected to color development treatment;
[0009] Wherein, the developing agent is chloroform, methanol and water in a volume ratio of 7:2.5:(0.2-0.25), or dichloromethane, ethyl acetate and methanol in a volume ratio of 1:(4.5-5.5):(3.5-4.5), or methanol, butanone, chloroform and water in a volume ratio of 2:(2.5-3.5):(2.5-3.5):(0.3-0.8);
[0010] The color developing agent used in the color developing treatment is an ethanol solution of aluminum chloride with a mass concentration of 0.5% to 5%.
[0011] In some embodiments, the developing agent is chloroform, methanol and water in a volume ratio of 7:2.5:0.25;
[0012] Or, the developing agent is chloroform, methanol and water in a volume ratio of 7:2.5:0.2;
[0013] Or, the developing solvent is dichloromethane, ethyl acetate and methanol in a volume ratio of 1:5:4;
[0014] Or, the developing agent is methanol, butanone, chloroform and water in a volume ratio of 2:3:3:0.5;
[0015] Optionally, the developing agent is chloroform, methanol and water in a volume ratio of 7:2.5:0.25.
[0016] In some embodiments, the developer is an ethanol solution of aluminum chloride with a mass concentration of 0.5% to 4%.
[0017] In some embodiments, the developer is an ethanol solution of aluminum chloride with a mass concentration of 1% to 3%.
[0018] In some of the embodiments, the color development process further includes a step of inspecting under an ultraviolet lamp, wherein the wavelength of the ultraviolet lamp is 365nm.
[0019] In some embodiments, the test solution of Cistanche deserticola and Epimedium black tea comprises the following steps:
[0020] Adding a first solvent to the Cistanche deserticola and Epimedium black tea to be tested for soaking and extraction, and evaporating the obtained extract to dryness to prepare a first solid;
[0021] The first solid is added into the second solvent to be dissolved, so as to prepare the test solution of the Cistanche deserticola and Epimedium black tea to be tested;
[0022] And / or, preparing the Cistanche deserticola control medicinal material solution comprises the following steps:
[0023] Adding a first solvent to a control herbal medicine of Epimedium to perform soaking extraction, and evaporating the obtained extract to dryness to prepare a first solid;
[0024] The first solid is added into the second solvent to dissolve, so as to prepare the Cistanche deserticola control medicinal material solution.
[0025] In some embodiments, the first solvent is independently an ethanol aqueous solution with a volume concentration of 60% to 80%.
[0026] In some embodiments, the second solvent is each independently methanol.
[0027] In some embodiments, adding the first solvent for extraction includes a soaking extraction step, wherein the soaking temperature is 65° C. to 75° C., and the soaking time is 1.5 h to 2.5 h.
[0028] In some embodiments, the temperature for evaporating the extract to dryness is 55°C to 65°C.
[0029] The above-mentioned thin layer chromatography detection method of epimedium in Cistanche epimedium black tea can effectively perform thin layer chromatography separation of epimedium in Cistanche epimedium black tea by improving the developing agent, color development method and thin layer plate type, and the separation effect is good. The detection has high specificity and high accuracy, which improves the accuracy and comprehensiveness of quality control of Cistanche epimedium black tea, and is conducive to the establishment and overall evaluation of quality standards for Cistanche epimedium black tea.
[0030] In addition, the above detection method also has the advantages of high sensitivity, simple operation, low cost and short detection time. BRIEF DESCRIPTION OF THE DRAWINGS
[0031] Figure 1 This is the thin layer plate image of Example 1 using chloroform-methanol-water (7:2.5:0.25) as the developing solvent.
[0032] Figure 2 This is the thin layer plate image of comparative example 1 using chloroform-methanol-water (7:1.5:0.3) as the developing solvent.
[0033] Figure 3 This is the thin layer plate image of comparative example 2 using chloroform-methanol-water (7:3:0.1) as the developing solvent.
[0034] Figure 4 This is the thin layer plate image of comparative example 3 using chloroform-methanol-water (7:2:0.25) as the developing solvent.
[0035] Figure 5 This is the thin layer plate image of Example 2 using chloroform-methanol-water (7:2.5:0.2) as the developing solvent.
[0036] Figure 6 This is the thin layer plate image of comparative example 4 using chloroform-methanol-water (8:1:0.5) as the developing solvent.
[0037] Figure 7 This is the thin layer plate image of comparative example 5 using chloroform-methanol-water (13:7:2) as the developing solvent.
[0038] Figure 8This is the thin layer plate image of comparative example 6 using chloroform-methanol-water (7:1.25:1.5) as the developing agent.
[0039] Fig. 9 This is the thin layer plate image of comparative example 7 using chloroform-methanol-water (7:0.25:2.5) as the developing solvent.
[0040] Fig.10 This is the thin layer plate image of Example 3 using dichloromethane-ethyl acetate-methanol (1:5:4) as the developing solvent.
[0041] Fig.11 This is the thin layer plate image of Example 4 using methanol-butanone-chloroform-water (2:3:3:0.5) as the developing solvent.
[0042] Fig.12 This is the thin layer plate image of comparative example 8 using chloroform-methanol-water (7:2.5:1.25) as the developing solvent.
[0043] Fig.13 This is the thin layer plate image of comparative example 9 using chloroform-methanol-water (7:2.5:1.5) as the developing solvent.
[0044] Fig.14 This is the thin layer plate image of comparative example 10 using chloroform-methanol-water (7:2.5:1.75) as the developing solvent.
[0045] Fig.15 This is the thin layer plate image of comparative example 11 using chloroform-methanol-water (7:2.5:2.25) as the developing agent.
[0046] Fig.16 This is the thin layer plate image of Comparative Example 12 using 0.1% aluminum chloride ethanol solution as the color developer.
[0047] Fig.17 This is the thin layer plate image of Example 5 using 0.5% aluminum chloride ethanol solution as the color developer.
[0048] Fig.18 This is the thin layer plate image of Example 6 using 2.0% aluminum chloride ethanol solution as the color developer.
[0049] Fig.19 This is the thin layer plate image of Example 7 using 3.0% aluminum chloride ethanol solution as the color developer.
[0050] Fig. 20 This is the thin layer plate image of Example 8 using 4.0% aluminum chloride ethanol solution as the color developer.
[0051] Fig.21This is the thin layer plate image of Comparative Example 13 using 5.0% aluminum chloride ethanol solution as the color developer.
[0052] Fig. 22 This is a thin layer plate diagram of comparative example 14 using a polyamide plate as the thin layer plate.
[0053] Fig.23 This is a thin layer plate diagram of Example 9 using a silica gel H plate as the thin layer plate.
[0054] Fig.24 This is a thin layer plate diagram of Example 10 using a silica gel GF254 plate as the thin layer plate.
[0055] Among them, the samples spotted on each thin layer plate are, from left to right, the test solution, the icariin reference solution, the epimedium reference medicinal material solution (the aforementioned solutions are spotted once) and the negative control medicinal material solution. DETAILED DESCRIPTION
[0056] The thin layer chromatography detection method of Epimedium in the Cistanche Epimedium black tea of the present application is further described in detail below in conjunction with specific examples. The present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thoroughly understood.
[0057] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.
[0058] The optional scope of the terms "and / or", "or / and", and "and / or" used in this article includes any one of two or more related listed items, and also includes any and all combinations of the related listed items, said any and all combinations include any combination of any two related listed items, any more related listed items, or all related listed items.
[0059] As used herein, "one or more" refers to any one, any two, or any two or more of the listed items.
[0060] In the present application, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.
[0061] In this application, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.
[0062] The percentage contents involved in this application, unless otherwise specified, refer to mass percentage for solid-liquid mixing and solid-solid mixing, and refer to volume percentage for liquid-liquid mixing.
[0063] The percentage concentrations mentioned in this application, unless otherwise specified, refer to the final concentration, which refers to the percentage of the added component in the system after the addition of the component.
[0064] The temperature parameters in this application, unless otherwise specified, allow for both constant temperature treatment and treatment within a certain temperature range. The constant temperature treatment allows the temperature to fluctuate within the accuracy range of instrument control.
[0065] The room temperature in the present application generally refers to 4°C to 30°C, preferably 20±5°C.
[0066] Some examples of the present application provide a thin layer chromatography detection method for epimedium in Cistanche epimedium black tea, comprising the following steps:
[0067] Prepare the test solution of Cistanche deserticola and Epimedium black tea, the icariin reference solution and the Epimedium reference medicinal material solution;
[0068] The test solution of the Cistanche deserticola and Epimedium black tea to be tested, the icariin reference solution and the Epimedium reference medicinal material solution are spotted on the same silica gel thin layer plate, developed with a developing agent, taken out, dried and then subjected to color development treatment;
[0069] The developing agent is chloroform, methanol and water in a volume ratio of 7:2.5:(0.2-0.25), or dichloromethane, ethyl acetate and methanol in a volume ratio of 1:(4.5-5.5):(3.5-4.5), or methanol, butanone, chloroform and water in a volume ratio of 2:(2.5-3.5):(2.5-3.5):(0.3-0.8); the color developer used in the color development treatment is an ethanol solution of aluminum chloride with a mass concentration of 0.5%-5%.
[0070] It can be understood that after the color development treatment, it is possible to judge whether the sample contains icariin and preliminarily determine its content based on the spot positions, colors and other characteristics of the test sample and the reference substance on the thin layer plate.
[0071] In some examples, the developing solvent is chloroform, methanol and water in a volume ratio of 7:2.5:0.25.
[0072] In some examples, the developing solvent is chloroform, methanol and water in a volume ratio of 7:2.5:0.2.
[0073] In some examples, the developing solvent is dichloromethane, ethyl acetate and methanol in a volume ratio of 1:5:4.
[0074] In some examples, the developing solvent is methanol, butanone, chloroform and water in a volume ratio of 2:3:3:0.5.
[0075] Without limitation, the silica gel thin layer plate may be, for example, a silica gel G plate, a silica gel H plate, or a silica gel GF254 plate, etc. In some examples, the silica gel thin layer plate is a silica gel G plate.
[0076] Specifically, the mass concentration of the ethanol solution of aluminum chloride includes, but is not limited to, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, or a range between any two of the foregoing. Further, the developer is an ethanol solution of aluminum chloride with a mass concentration of 0.5% to 4%. Further, the developer is an ethanol solution of aluminum chloride with a mass concentration of 1% to 3%.
[0077] In some of the examples, the color development process includes a step of observing under an ultraviolet lamp. Further, the wavelength of the ultraviolet lamp is 365nm.
[0078] In addition, the accuracy and sensitivity of the detection can be further improved by effectively extracting the icariin component in the Cistanche deserticola and Epimedium black tea to be tested.
[0079] In some of these examples, the test solution of Cistanche deserticola and Epimedium black tea includes the following steps:
[0080] Adding a first solvent to the Cistanche deserticola and Epimedium black tea to be tested for soaking and extraction, and evaporating the obtained extract to dryness to prepare a first solid;
[0081] The first solid is added into the second solvent to be dissolved, so as to prepare the test solution of the Cistanche deserticola and Epimedium black tea to be tested.
[0082] In some of the examples, preparing the Cistanche deserticola control medicinal material solution includes the following steps:
[0083] Adding a first solvent to a control herbal medicine of Epimedium to perform soaking extraction, and evaporating the obtained extract to dryness to prepare a first solid;
[0084] The first solid is added into the second solvent to dissolve, so as to prepare the Cistanche deserticola control medicinal material solution.
[0085] In some examples, the first solvent is independently an ethanol aqueous solution with a volume concentration of 60% to 80%. Specifically, the volume concentration of the ethanol aqueous solution includes but is not limited to: 60%, 63%, 65%, 67%, 70%, 73%, 75%, 77%, 80% or a range between any two of the foregoing.
[0086] In some examples, the second solvent is each independently methanol.
[0087] In some examples, adding the first solvent for extraction includes a soaking extraction step, wherein the soaking temperature is 65°C to 75°C, and the soaking time is 1.5h to 2.5h. Specifically, the soaking temperature includes, but is not limited to, 65°C, 67°C, 70°C, 73°C, 75°C, or a range between any two of the foregoing; the soaking time includes, but is not limited to, 1.5h, 2h, 2.5h, or a range between any two of the foregoing.
[0088] In some examples, the temperature at which the extract is evaporated to dryness is 55° C. to 65° C. Specifically, the temperature at which the extract is evaporated to dryness includes, but is not limited to, 55° C., 57° C., 60° C., 63° C., 65° C., or a range between any two of the foregoing.
[0089] For experimental parameters not specified in the following specific embodiments, reference is made to the instructions given in the present application document, and reference may also be made to experimental manuals in the art or other experimental methods known in the art, or to experimental conditions recommended by manufacturers.
[0090] The raw materials and reagents involved in the following specific embodiments can be obtained from the market, or those skilled in the art can prepare them according to known means. The Cistanche Epimedium Black Tea used in the embodiment is from Xinjiang Huachun Pharmaceutical Co., Ltd., with the following proportions: Cistanche Tubulosa 1410g, jujube 990g, epimedium 990g, Acanthopanax Senticosus 990g, black tea 2010g, dextrin 99g, and this product is a 3.0g / bag bagged tea brewing agent.
[0091] Example 1
[0092] This embodiment is a thin layer chromatography detection method for epimedium in Cistanche deserticola epimedium black tea.
[0093] Take 1 g each of 3 batches of Cistanche epimedium black tea, epimedium control medicinal materials, and negative control medicinal materials (4 kinds of medicinal materials, Cistanche tubulosa, jujube, Acanthopanax and black tea, are mixed and crushed according to the compound ratio), add 20 mL of 70% ethanol aqueous solution, soak at 70°C for 2 hours, filter, evaporate the filtrate at 60°C, and dissolve the residue in 1 mL of methanol as the test solution, epimedium control medicinal material solution and negative control medicinal material solution.
[0094] Take the icariin reference substance again, add methanol to make a solution containing 1 mg per 1 mL, which is used as the icariin reference substance solution.
[0095] According to the thin layer chromatography method (General Rules 0502 of the 2020 edition of the Chinese Pharmacopoeia), 1 μL of each of the above four solutions was taken and spotted on the same silica gel G thin layer plate, and chloroform-methanol-water (7:2.5:0.25) was used as the developing solvent. The plate was developed, taken out, dried, sprayed with 1% aluminum chloride ethanol solution, and examined under ultraviolet light (365 nm). The results are as follows: Figure 1 shown.
[0096] Examples 2 to 4 and Comparative Examples 1 to 11
[0097] This embodiment and comparative example are thin layer chromatography detection methods for epimedium in Cistanche epimedium black tea. The steps are the same as those in embodiment 1, and the main difference is that different developing agents are used. The specific developing agents are shown in Table 1 below. The detection results are shown in Table 1 below. Figures 2 to 15 As shown:
[0098] Table 1
[0099]
[0100]
[0101] Combination Figures 1 to 15 It can be seen that the developing agents of Examples 1 to 4 can effectively separate icariin, a characteristic ingredient of epimedium medicinal materials in Cistanche epimedium black tea, and the spots are clear, the interference of miscellaneous peaks is small, and the detection results are accurate. Among them, Examples 1, 3, and 4 are better. Compared with the three, the development distance of Example 1 is more appropriate. Comparative Examples 1 to 11 change the type of developing agent, and there are problems such as overlap with adjacent spots, unclear spots, chaotic arrangement, and excessive development, which makes it difficult to detect epimedium in Cistanche epimedium black tea.
[0102] Examples 5 to 8 and Comparative Examples 12 to 13
[0103] This embodiment and comparative example are thin layer chromatography detection methods for epimedium in Cistanche epimedium black tea. The steps are the same as those in embodiment 1, and the main difference is that different color developers are used. The specific color developers are shown in Table 2 below. The detection results are shown in Table 2 below. Figures 16 to 21 As shown:
[0104] Table 2
[0105] Color developer Comparative Example 12 0.1% aluminum chloride ethanol solution Example 5 0.5% aluminum chloride ethanol solution Example 6 2.0% aluminum chloride ethanol solution Example 7 3.0% aluminum chloride ethanol solution Example 8 4.0% Aluminum chloride ethanol solution Comparative Example 13 5.0% aluminum chloride ethanol solution
[0106] Combination Figure 1 and Figures 16 to 21 It can be seen that when the mass concentration of the aluminum chloride ethanol solution is too low, it is difficult to effectively color the spots, which is not conducive to accurate detection. When the mass concentration of the aluminum chloride ethanol solution is too high, the impurity spots are also colored heavily, interfering with the detection of the spots. Therefore, it is necessary to reasonably control the mass concentration of the aluminum chloride ethanol solution to 0.5% to 5%, preferably 0.5% to 4%, and more preferably 1% to 3%.
[0107] Examples 9-10 and Comparative Example 14
[0108] This embodiment and comparative example are thin layer chromatography detection methods for epimedium in Cistanche epimedium black tea. The steps are the same as those in embodiment 1, and the main difference is that different thin layer plates are used. The specific thin layer plates are shown in Table 3 below. The detection results are shown in Table 3 below. Figures 22-24 As shown;
[0109] Table 3
[0110] Thin layer plate Comparative Example 14 Polyamide board Example 9 Silicone H plate Example 10 Silicone GF254 board
[0111] Combination Figure 1 and 22 It can be seen from Figures 24 to 24 that different types of silica gel plates are used in Examples 1, 9 to 10, which can effectively separate icariin, a characteristic ingredient of epimedium medicinal materials in Cistanche epimedium black tea, with clear spots, little interference from miscellaneous peaks, and accurate detection results, among which Example 1 is the best. Comparative Example 14 uses a polyamide plate, and the spots are relatively chaotic after development, making it difficult to detect epimedium in Cistanche epimedium black tea.
[0112] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
[0113] The above-described embodiments only express several implementation methods of the present application, which is convenient for understanding the technical solution of the present application in detail, but it cannot be understood as limiting the scope of protection of the patent application. It should be pointed out that for ordinary technicians in this field, without departing from the concept of the present application, several modifications and improvements can be made, which all belong to the scope of protection of the present application. It should be understood that the technical solutions obtained by those skilled in the art through logical analysis, reasoning or limited experiments on the basis of the technical solutions provided in the present application are all within the scope of protection of the claims attached to the present application. Therefore, the scope of protection of the patent of this application shall be based on the content of the attached claims, and the description can be used to interpret the content of the claims.
Claims
1. A thin layer chromatography detection method for epimedium in Cistanche deserticola and epimedium black tea, characterized in that: The steps include: Prepare the test solution of Cistanche deserticola and Epimedium black tea, the icariin reference solution and the Epimedium reference medicinal material solution; The test solution of the Cistanche deserticola and Epimedium black tea to be tested, the icariin reference solution and the Epimedium reference medicinal material solution are spotted on the same silica gel thin layer plate, developed with a developing agent, taken out, dried and then subjected to color development treatment; Wherein, the developing agent is chloroform, methanol and water in a volume ratio of 7:2.5:(0.2-0.25), or dichloromethane, ethyl acetate and methanol in a volume ratio of 1:(4.5-5.5):(3.5-4.5), or methanol, butanone, chloroform and water in a volume ratio of 2:(2.5-3.5):(2.5-3.5):(0.3-0.8); The color developing agent used in the color developing treatment is an ethanol solution of aluminum chloride with a mass concentration of 0.5% to 5%.
2. The thin layer chromatography detection method of epimedium in Cistanche deserticola and epimedium black tea according to claim 1, characterized in that: The developing solvent is chloroform, methanol and water in a volume ratio of 7:2.5:0.25; Or, the developing agent is chloroform, methanol and water in a volume ratio of 7:2.5:0.2; Or, the developing solvent is dichloromethane, ethyl acetate and methanol in a volume ratio of 1:5:4; Or, the developing agent is methanol, butanone, chloroform and water in a volume ratio of 2:3:3:0.5; Optionally, the developing agent is chloroform, methanol and water in a volume ratio of 7:2.5:0.
25.
3. The thin layer chromatography detection method of epimedium in Cistanche deserticola and epimedium black tea according to claim 1, characterized in that: The color developer is an ethanol solution of aluminum chloride with a mass concentration of 0.5% to 4%.
4. The thin layer chromatography detection method of epimedium in Cistanche deserticola and epimedium black tea according to claim 3, characterized in that: The color developer is an ethanol solution of aluminum chloride with a mass concentration of 1% to 3%.
5. The thin layer chromatography detection method of epimedium in Cistanche deserticola and epimedium black tea according to claim 1, characterized in that: After the color development process, the step of inspecting under ultraviolet light is also included, and the wavelength of the ultraviolet light is 365nm.
6. The thin layer chromatography detection method of epimedium in Cistanche deserticola and epimedium black tea according to any one of claims 1 to 5, characterized in that: The test solution of Cistanche deserticola and Epimedium black tea to be tested comprises the following steps: Adding a first solvent to the Cistanche deserticola and Epimedium black tea to be tested for soaking and extraction, and evaporating the obtained extract to dryness to prepare a first solid; The first solid is added into the second solvent to be dissolved, so as to prepare the test solution of the Cistanche deserticola and Epimedium black tea to be tested; And / or, preparing the Cistanche deserticola control medicinal material solution comprises the following steps: Adding a first solvent to a control herbal medicine of Epimedium to perform soaking extraction, and evaporating the obtained extract to dryness to prepare a first solid; The first solid is added into the second solvent to dissolve, so as to prepare the Cistanche deserticola control medicinal material solution.
7. The thin layer chromatography detection method of epimedium in Cistanche deserticola and epimedium black tea according to claim 6, characterized in that: The first solvents are each independently an ethanol aqueous solution with a volume concentration of 60% to 80%.
8. The thin layer chromatography detection method of epimedium in Cistanche deserticola and epimedium black tea according to claim 6, characterized in that: The second solvent is each independently methanol.
9. The thin layer chromatography detection method of epimedium in Cistanche deserticola and epimedium black tea according to claim 6, characterized in that: Adding the first solvent for extraction includes a soaking extraction step, wherein the soaking temperature is 65° C. to 75° C., and the soaking time is 1.5 h to 2.5 h.
10. The thin layer chromatography detection method of epimedium in Cistanche deserticola and epimedium black tea according to claim 6, characterized in that: The temperature for evaporating the obtained extract to dryness is 55°C to 65°C.
Citation Information
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Thin-layer chromatography detection method for herba epimedii in ginseng-radix puerariae kidney-tonifying preparation
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