A light path transmission mechanism and an optical circuit module of a dry fluorescent immunoassay analyzer

Abstract

The present application provides an optical path transmission mechanism and an optical circuit module of a dry fluorescent immunoassay analyzer, wherein the optical path transmission mechanism comprises a light source (5), and further comprises: a first convex mirror (11), arranged at a first relative position of the light source (5), so that the light emitted by the light source (5) can be transmitted to a reagent strip reactant through the first convex mirror (11); a second convex mirror (9), arranged at a second relative position of the first convex mirror (11), so that the reflected light of the reagent strip reactant can be transmitted to the second convex mirror (9) through the first convex mirror (11), and then transmitted out through the second convex mirror (9). In the technical solution provided by the present application, the intensity of the optical signal is improved, and the uncertainty of the measurement result of the dry fluorescent immunoassay analyzer is reduced.

Description

Technical Field

[0001] The present invention relates to the field of optoelectronic technology, and in particular to an optical path transmission mechanism and an optical circuit module of a dry fluorescent immunoassay analyzer. Background Art

[0002] The dry fluorescent immunoassay analyzer has good repeatability and accuracy, a wide linear range, is sensitive, fast, precise and reliable. It is a simple and efficient POCT quantitative detection device, mainly used for in vitro detection. It is widely used in central laboratories, outpatient / emergency laboratories, clinical departments and other medical service points, physical examination centers, etc. of medical institutions, and is also suitable for scientific research laboratory testing.

[0003] The optical circuit module is used in conjunction with the fluorescent immunochromatographic analysis reagent card. By measuring the T line and C line at different positions on the reagent strip, the signal transmission analysis is realized to judge the in vitro detection. This module is the core component of the fluorescent immunochromatographic analysis instrument, and the accuracy of this component directly affects the judgment of the instrument.

[0004] As the core component of the dry fluorescent immunoassay analyzer, the performance of the optical circuit module directly affects the final performance of the dry fluorescent immunoassay analyzer. The optical circuit module of the existing dry fluorescent immunoassay analyzer uses silicon photocells as optical signal receiving devices. The working principle of silicon photocells is based on the photoelectric effect, that is, when light is irradiated to the surface of the silicon photocell, photons can excite electrons in the silicon material, thereby generating current. The magnitude of this current is proportional to the light intensity irradiated on the silicon photocell. Therefore, when the silicon photocell receives a stable and sufficiently strong light signal, it can generate a significant current output, thereby achieving efficient photoelectric conversion. The working principle of silicon photocells is based on the photoelectric effect, that is, when light is irradiated to the surface of the silicon photocell, photons can excite electrons in the silicon material, thereby generating current. The magnitude of this current is proportional to the light intensity irradiated on the silicon photocell. Therefore, when the silicon photocell receives a stable and sufficiently strong light signal, it can generate a significant current output, thereby achieving efficient photoelectric conversion. When a weak signal is received, due to the low energy of the weak signal, the number of excited electrons is limited, resulting in a very weak current generated by the silicon photocell. This weak current can be easily masked by noise in the subsequent electrical signal processing, thereby increasing the uncertainty of the measurement results of the dry fluorescent immunoassay analyzer. Summary of the invention

[0005] In view of this, one of the purposes of the embodiments of the present application is to provide a light path transmission mechanism to improve the uncertainty of the measurement results of the dry fluorescent immunoassay analyzer.

[0006] In order to achieve the above technical objectives, the technical solutions adopted in this application are as follows: In a first aspect, an embodiment of the present application provides a light path transmission mechanism, including a light source, and further including: A first convex mirror is arranged at a first relative position of the light source, so that the light emitted by the light source can be transmitted to the reagent in the reagent strip through the first convex mirror; The second convex mirror is arranged at a second relative position of the first convex mirror, so that the reflected light of the reagent of the reagent strip can be transmitted to the second convex mirror through the first convex mirror and then transmitted out through the second convex mirror.

[0007] Furthermore, it also includes a dichroic mirror, which is arranged at a third relative position of the first convex mirror, so that the light emitted by the light source can be refracted to the first convex mirror through the dichroic mirror.

[0008] Furthermore, the third relative position is a position between the first convex mirror and the second convex mirror, so that the reflected light can be transmitted to the second convex mirror through the first convex mirror and the dichroic mirror in sequence.

[0009] Furthermore, it also includes a collimator, which is arranged between the light source and the dichroic mirror. The light emitted by the light source passes through the collimator and is then transmitted to the dichroic mirror.

[0010] Furthermore, it also includes a filter, which is arranged between the second convex mirror and the dichroic mirror, so that the reflected light can pass through the first convex mirror, the dichroic mirror and the filter and be transmitted to the second convex mirror.

[0011] Furthermore, it also includes a circular plastic sheet, which is arranged at a fourth relative position of the second convex mirror, so that the reflected light passes through the first convex mirror and the second convex mirror in sequence and is transmitted to the circular plastic sheet, and then passes out through the circular plastic sheet.

[0012] In a second aspect, an embodiment of the present application provides an optical circuit module of a dry fluorescent immunoassay analyzer, comprising the above-mentioned optical path transmission mechanism, and further comprising: A light source PCB board, on which the light source of the light path transmission mechanism is installed, so that when the light source PCB board is powered on, the light source can emit light.

[0013] an optical signal processing module, configured to receive the reflected light transmitted through the second convex mirror, and process the optical signal corresponding to the reflected light into an electrical signal; The signal receiving PCB board is connected to the optical signal processing module and can receive and transmit the electrical signal.

[0014] Furthermore, it also includes a shell, the light path transmission mechanism is arranged inside the shell, and the light source PCB board and the signal receiving PCB board are both clamped on the shell.

[0015] Furthermore, the light source PCB board and the signal receiving PCB board are arranged vertically, and the light source PCB board and the signal receiving PCB board are welded.

[0016] Furthermore, the optical signal processing module includes a photodiode.

[0017] The invention adopting the above technical solution has the following advantages: In the technical solution provided in the present application, a first convex mirror and a second convex mirror are provided, and the light emitted by the light source is emitted toward the reagent strip reactant after passing through the first convex mirror, and the light reflected by the reagent strip reactant is also emitted toward the second convex mirror through the first convex mirror, and is emitted after passing through the second convex mirror, that is, three-way focusing of the light is achieved by the first convex mirror and the second convex mirror, thereby shortening the path length of the light transmission, improving the intensity of the light signal, and reducing the uncertainty of the measurement results of the dry fluorescent immunoassay analyzer.

[0018] In the technical solution provided in the present application, the optical path transmission mechanism, the light source PCB board, the optical signal processing module and the receiving signal PCB board are integrated on the shell, forming a compact and fully functional optical circuit module, thereby improving the integration and reliability of the optoelectronic module. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The present application may be further described by the non-limiting embodiments given in the accompanying drawings. It should be understood that the following drawings only illustrate certain embodiments of the present application and therefore should not be regarded as limiting the scope. For those of ordinary skill in the art, other relevant drawings may be obtained based on these drawings without creative effort.

[0020] Figure 1 A schematic diagram of the structure provided for an embodiment of the present application.

[0021] Figure 2 A schematic diagram of the optical path provided in an embodiment of the present application.

[0022] Icon: 1-housing; 2-optical signal processing module; 3-receiving signal PCB board; 4-light source PCB board; 5-light source; 6-collimating mirror; 7-dichroic mirror; 8-filter; 9-second convex mirror; 10-circular plastic sheet; 11-first convex mirror; 12-reagent strip reactant. DETAILED DESCRIPTION

[0023] The present application will be described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be noted that in the drawings or descriptions, similar or identical parts use the same figure numbers, and the implementation methods not shown or described in the drawings are forms known to ordinary technicians in the relevant technical field. In the description of this application, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance. Example 1

[0024] This embodiment proposes a light path transmission mechanism, such as Figure 1 As shown, it includes a light source 5, which can emit light after being powered on. In this embodiment, the light source 5 can emit ultraviolet light, and the light source 5 can be a lamp bead. The light path transmission mechanism can guide the light emitted by the light source 5 to the reagent strip reactant 12, and transmit the light reflected by the reagent strip reactant 12.

[0025] The reagent strip reactants include the target analyte in the sample to be tested and the specific chemical substances on the reagent strip (such as fluorescently labeled antibodies, capture antibodies, etc.). These reactants interact with each other during the detection process and jointly determine the accuracy and reliability of the test results. The sample to be tested is placed on the reagent strip. The target analyte in the sample binds to the capture antibody on the reagent strip to form an immune complex. Subsequently, the fluorescently labeled antibody binds to the immune complex to form an immune complex with a fluorescent marker. When the light emitted by the light source 5 shines on the reagent strip, the fluorescent marker emits a fluorescent signal. The fluorescence detection system inside the dry fluorescent immunoassay analyzer captures these signals and converts them into electrical signals for processing and analysis, and finally obtains the test results.

[0026] The optical path transmission mechanism includes a collimating mirror 6 , a dichroic mirror 7 , a filter 8 , a second convex mirror 9 , a circular plastic sheet 10 and a first convex mirror 11 .

[0027] The first convex mirror 11 is arranged at a first relative position of the light source 5, so that the light emitted by the light source 5 can be transmitted to the reagent strip reactant 12 through the first convex mirror 11. The second convex mirror 9 is arranged at a second relative position of the first convex mirror 11, so that the reflected light of the reagent strip reactant 12 can be transmitted to the second convex mirror 9 through the first convex mirror 11 and transmitted out through the second convex mirror 9.

[0028] The first convex mirror 11 and the second convex mirror 9 both have the function of amplifying optical signals. When the light emitted by the light source 5 is transmitted to the reagent strip reactant 12 through the first convex mirror 11, the optical signal corresponding to the light is amplified for the first time when passing through the first convex mirror 11, and the reflected light of the reagent strip reactant 12 will pass through the first convex mirror 11 and the second convex mirror 9. At this time, the optical signal corresponding to the reflected light is amplified twice. Therefore, the arrangement of the first convex mirror 11 and the second convex mirror 9 enables the optical signal to be amplified three times during the transmission process, thereby improving the intensity of the optical signal, shortening the path length of the light transmission, and thereby improving the sensitivity of the dry fluorescent immunoassay analyzer.

[0029] In this embodiment, the first relative position indicates that the first convex mirror 11 is located at the side of the light source 5 , and the second relative position indicates that the second convex mirror 9 is located at the bottom of the first convex mirror 11 .

[0030] The dichroic mirror 7 is arranged at the third relative position of the first convex mirror 11, so that the light emitted by the light source 5 can be refracted to the first convex mirror 11 through the dichroic mirror 7. The dichroic mirror 7 is used to separate the light source into a specific spectrum. When the incident angle is 45 degrees or large, the light path direction of part of the spectrum is changed to pass the light of a specific wavelength. The dichroic mirror 7 can selectively transmit or reflect light according to the wavelength, realizing flexible switching of the light path.

[0031] The third relative position in this embodiment means that the dichroic mirror 7 is arranged between the first convex mirror 11 and the second convex mirror 9, which can save space for arranging the dichroic mirror 7, the first convex mirror 11 and the second convex mirror 9.

[0032] The collimator 6 is arranged between the light source 5 and the dichroic mirror 7. The collimator 6 is used to convert the light from the light source 5 incident on the collimator 6 into a parallel light beam for emission, thereby improving the parallelism and focusing performance of the light.

[0033] The collimator 6 is an optical element that can convert incident light into parallel light or quasi-parallel light. The collimator makes the light perpendicular to the mirror surface to ensure that the directions of the incident light and the reflected light are exactly the same, thereby achieving the collimation of the light beam. The collimator usually consists of a lens barrel and a field lens. The field lens is located in the front of the collimator tube and is used to check the direction of the target, expand the target field of view, and help to accurately calibrate and measure the target.

[0034] The filter 8 is disposed between the second convex mirror 9 and the dichroic mirror 7, so that the light reflected by the reagent 12 of the reagent strip can pass through the first convex mirror 11, the dichroic mirror 7 and the filter 8 in sequence and be transmitted to the second convex mirror 9. When the light passes through the filter 8, the filter membrane of the filter 8 selectively absorbs or transmits the light within a specific wavelength range.

[0035] The setting of the filter 8 can further filter out unnecessary light and improve the purity and quality of the reflected light. For subsequent optical signal processing, the optical signal can be received and processed more effectively, and the accuracy and reliability of the analysis can be improved.

[0036] The circular plastic sheet 10 is arranged at the fourth relative position of the second convex mirror 9, so that the reflected light passes through the first convex mirror 11 and the second convex mirror 9 in sequence and then is transmitted to the circular plastic sheet 10, and then is transmitted out through the circular plastic sheet 10. The circular plastic sheet 10 uses the rectilinear propagation of light. Light propagates along a straight line in the same uniform material. The closer the distance to the object, the larger the image and the darker the brightness; the farther the distance to the object, the smaller the image and the brighter the brightness. The fourth relative position in this embodiment indicates that the round plastic sheet 10 is arranged above the second convex mirror 9 .

[0037] In this embodiment, the arrangement of the first convex mirror and the second convex mirror realizes efficient transmission of light from the light source to the reagent strip reactant and then to the reflected light. This design ensures the stability and accuracy of the light during transmission, improves the efficiency of the light path, and also increases the intensity of the light signal. Example 2

[0038] This embodiment proposes an optical circuit module of a dry fluorescent immunoassay analyzer, such as Figure 1 As shown, it includes a shell 1, a receiving signal PCB board 3 and a light source PCB board 4. The receiving signal PCB board 3 and the light source PCB board 4 are clamped on the shell 1, and the receiving signal PCB board 3 and the light source PCB board 4 are arranged vertically. A light source 5 is installed on the light source PCB board 4. When the light source PCB board 4 is powered on, the light source 5 can emit light.

[0039] In this embodiment, the housing 1 may include an upper shell and a lower shell, which are connected and fixed to form a whole. The upper shell and the lower shell are designed with corresponding notches to limit the receiving signal PCB board 3 and the light source PCB board 4.

[0040] The signal receiving PCB board 3 is connected to the optical signal processing module 2 and is used for transmitting the electrical signal sent by the optical signal processing module 2 .

[0041] The light source PCB board 4 is used for emitting light from the light source 5 so that the light source 5 emits light of a certain wavelength.

[0042] In this embodiment, the optical signal processing module is used to receive the reflected light transmitted through the second convex mirror 9 and process the optical signal corresponding to the reflected light into an electrical signal. The circular plastic sheet is arranged between the optical signal processing module 2 and the second convex mirror 9. The optical signal processing module 2 is a photodiode.

[0043] In this embodiment, the optical path transmission mechanism described in Embodiment 1 is located below the optical signal processing module 2 , and the optical path transmission mechanism can transmit the reflected light of the reagent 12 of the reagent strip to the optical signal processing module 2 .

[0044] In this embodiment, a guide hole can be provided in the shell 1, and the first convex mirror 11 is located above the guide hole. When in use, the reagent strip reactant 12 is placed below the guide hole. The guide hole guides the light focused by the first convex mirror 11 to the reagent strip reactant 12, and the light reflected by the reagent strip reactant 12 is then guided to the optical signal processing module 2 via the guide hole, the first convex mirror 11, the dichroic mirror 7, and the second convex mirror 9.

[0045] In this embodiment, a limited slot can be set inside the shell 1, and the collimating mirror 6, dichroic mirror 7, filter 8, second convex mirror 9, circular plastic sheet 10 and first convex mirror 11 are placed in the corresponding slot positions to achieve the installation of various components.

[0046] In this embodiment, the light source PCB board 4 and the light source 5 are welded and integrated, and the light source PCB board 4 and the signal receiving PCB board are also welded and connected; the two are arranged vertically on the housing 1. The housing 1 is designed with a limit card slot according to the size of the light source 5 to ensure that the position of the lamp beads can be accurately guaranteed every time it is installed. When installing the light source PCB board 4, it can be directly placed on the housing along the corresponding limit card slot to achieve limit.

[0047] The collimator 6 is placed inside the corresponding circular groove of the housing 1 and is located directly in front of the light source 5 in order to convert the scattered light emitted by the light source 5 into parallel light, which is beneficial to the transmission of the subsequent light beam and the processing of the reagent reactant 12.

[0048] The dichroic mirror 7 is arranged inside the corresponding square groove of the housing 1, and the dichroic mirror 7 is arranged at a 45-degree angle with the collimator 6, the purpose of which is to change the irradiation direction of the light irradiated to the dichroic mirror 7. The light source 5 emits light, and then passes through the collimator 6 to change the light beam into parallel light, which is irradiated to the dichroic mirror 7, and the dichroic mirror refracts the parallel light.

[0049] like Figure 2 As shown, the entire working principle of the device is as follows: the light source PCB board 4 is electrically excited to emit ultraviolet light by the lamp bead 5, and the scattered light becomes parallel light after passing through the collimator 6, and is directly irradiated onto the dichroic mirror 7. The dichroic mirror 7 is arranged at 45 degrees. The ultraviolet light is refracted by the dichroic mirror 7 and irradiated onto the first convex mirror 9. The light is focused by the first convex mirror 9 and hits the reagent strip reactant 12, and the reactant excitation light is reflected to the first convex mirror 9. The specific wavelength light is transmitted through the dichroic mirror 7 to the filter 8, and the remaining wavelength clutter is filtered again to reach the second convex mirror 9, through the focused light, and finally through the circular plastic sheet 10 to pass through the stray light to reach the optical signal processing module 2 for signal conversion, and the signal is transmitted through the receiving signal PCB board 3 to achieve the purpose of detecting and reading the signal.

[0050] The above description is only an embodiment of the present application and is not intended to limit the protection scope of the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A light path transmission mechanism, comprising a light source, characterized in that: Also includes: A first convex mirror (11) is arranged at a first relative position to the light source (5), so that the light emitted by the light source (5) can be transmitted to the reagent strip reactant through the first convex mirror (11); The second convex mirror (9) is arranged at a second relative position of the first convex mirror (11), so that the reflected light of the reagent of the reagent strip can be transmitted to the second convex mirror (9) through the first convex mirror (11) and then transmitted out through the second convex mirror (9).

2. The optical path transmission mechanism according to claim 1, characterized in that: It also comprises a dichroic mirror (7), which is arranged at a third relative position of the first convex mirror (11) so that light emitted by the light source can be refracted to the first convex mirror (11) through the dichroic mirror (7).

3. The optical path transmission mechanism according to claim 2, characterized in that: The third relative position is the position between the first convex mirror (11) and the second convex mirror, so that the reflected light can be transmitted to the second convex mirror (9) through the first convex mirror (11) and the dichroic mirror (7) in sequence.

4. The optical path transmission mechanism according to claim 2, characterized in that: It also comprises a collimator (6), wherein the collimator (6) is arranged between the light source and the dichroic mirror (7), and the light emitted by the light source passes through the collimator (6) and is then transmitted to the dichroic mirror (7).

5. The light path transmission mechanism according to claim 2, characterized in that: It also comprises a filter (8), wherein the filter (8) is arranged between the second convex mirror (9) and the dichroic mirror (7), so that the reflected light can pass through the first convex mirror (11), the dichroic mirror (7) and the filter (8) and be transmitted to the second convex mirror (9).

6. The light path transmission mechanism according to claim 1, characterized in that: It also comprises a circular plastic sheet (10), the circular plastic sheet (10) being arranged at a fourth relative position of the second convex mirror (9), so that the reflected light passes through the first convex mirror (11) and the second convex mirror (9) in sequence, is transmitted to the circular plastic sheet (10), and is transmitted out through the circular plastic sheet (10).

7. An optical circuit module for a dry fluorescent immunoassay analyzer, characterized in that: The optical path transmission mechanism comprises the optical path transmission mechanism according to any one of claims 1 to 6, further comprising: A light source PCB board (4), on which the light source of the light path transmission mechanism is mounted, so that when the light source PCB board (4) is powered on, the light source can emit light; An optical signal processing module (2) is used to receive the reflected light transmitted through the second convex mirror (9), and process the optical signal corresponding to the reflected light into an electrical signal; The signal receiving PCB board (3) is connected to the optical signal processing module (2) and is capable of receiving and transmitting the electrical signal.

8. The optical circuit module of the dry fluorescent immunoassay analyzer according to claim 7, characterized in that: It also comprises a housing (1), the light path transmission mechanism being arranged inside the housing (1), and the light source PCB board (4) and the signal receiving PCB board (3) being both snap-connected to the housing (1).

9. The optical circuit module of the dry type fluorescent immunoassay analyzer according to claim 7, characterized in that: The light source PCB board (4) and the signal receiving PCB board (3) are arranged vertically, and the light source PCB board (4) and the signal receiving PCB board (3) are welded.

10. The optical circuit module of the dry type fluorescent immunoassay analyzer according to claim 7, characterized in that: The optical signal processing module (2) comprises a photodiode.

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