Electric microscope and control method thereof

Abstract

The invention discloses an electric microscope, belongs to the technical field of biological microscopes, and solves the problems that structural parameters of an existing electric microscope cannot be adjusted, an objective table shakes in the focusing motion process, and a specimen slide is easy to crush. The device comprises a base and a supporting vertical plate, the other side of the supporting vertical plate is provided with an electric mechanism for controlling the collecting lens assembly and the objective table to ascend and descend, the electric mechanism comprises a main lifting motor, a guide rail II, a ball screw I and a sliding block assembly I, the output end of the main lifting motor is connected with the ball screw I, the sliding block assembly I is installed on the guide rail II in a sliding mode, and the sliding block assembly I is in threaded connection with the ball screw I; and the slide block assembly I is in threaded connection with a pressure adjusting screw. According to the electric microscope, the front-and-back and left-and-right moving range of the specimen slide is widened, the risk that the specimen slide is crashed or a mechanical structure is damaged through electric control is reduced, and the influence of shaking in the lifting process of the objective table is reduced. The invention also discloses an electric microscope control method.

Description

Technical Field

[0001] The present invention relates to the technical field of biological microscopes, and more specifically, to an electric microscope and a control method thereof. Background Art

[0002] Biological microscopes have been widely used in education, scientific research, medical treatment, environmental science and other fields. For example, biomedical observation of cell biology, analysis of biological tissue sections and study of disease states. Currently, automated microscopes have been manufactured that have the functions of automatically controlling the movement of the stage, moving the stage at multiple angles, automatically acquiring clear images, and automatically analyzing data. However, these developed automated microscope systems are usually produced abroad, which are not only expensive, but also have extremely high operating requirements.

[0003] Domestic electric microscopes, such as the applicant's prior application, publication number: CN 114428393 B, disclose a mechanical transmission structure of a fully automatic biological microscope, including a base and a stage, the stage includes an upper support plate, a middle support plate and a lower support plate, the base is provided with a first transmission mechanism that can reciprocate up and down, the lower support plate is connected to the output end of the first transmission mechanism, the lower support plate is provided with a second transmission mechanism that can reciprocate forward and backward, the middle support plate is connected to the output end of the second transmission mechanism, the middle support plate is provided with a third transmission mechanism that can reciprocate left and right, the upper support plate is connected to the output end of the third transmission mechanism; the middle part between the upper support plate, the middle support plate and the lower support plate is provided with mutually penetrating light holes. The mechanical transmission structure of the fully automatic biological microscope of the present invention can realize the adjustment of the stage in the front and back, up and down, left and right directions, and is more convenient to use.

[0004] The electric control of the existing electric microscope has the risk of breaking the specimen slide or damaging the mechanical structure, and there is shaking during the lifting and lowering of the stage, which is not conducive to the use of software algorithms.

[0005] Therefore, it is urgent to develop and design an electric microscope and a control method thereof to solve the above-mentioned technical problems. Summary of the invention

[0006] The technical problem to be solved by the present invention is to address the above-mentioned shortcomings of the prior art. The first purpose of the present invention is to provide an electric microscope to increase the forward and backward and left and right movement range of the specimen slide, reduce the risk of electric control breaking the specimen slide or damaging the mechanical structure, and reduce the impact of shaking during the lifting and lowering of the stage.

[0007] The second purpose of the present invention is to provide a method for controlling an electric microscope to achieve rapid detection of specimens.

[0008] In order to achieve the above-mentioned purpose one, the present invention provides an electric microscope, including a base and a supporting plate, the supporting plate is vertically fixed on the surface of the base, a guide rail I is fixed on one side of the supporting plate, four sliders are slidably installed on the guide rail I, and the four sliders are distributed from bottom to top and are respectively fixed with a light source assembly, a condenser assembly, a stage and an objective lens eyepiece assembly; the other side of the supporting plate is provided with an electric mechanism for controlling the lifting of the condenser assembly and the stage, the electric mechanism includes a main lifting motor, a guide rail II, a ball screw I and a slider assembly I, the main lifting motor and guide rail II are both fixed on the supporting plate, and the output end of the main lifting motor is connected to the ball screw I, the slider assembly I is slidably installed on the guide rail II, and the slider assembly I is threadedly connected with the ball screw I, the slider assembly I is threadedly connected with a pressure adjustment screw, the pressure adjustment screw is sleeved with a spring I, the pressure adjustment screw on one side of the spring I is sleeved with a slider assembly II that is slidably connected to the guide rail II, a condenser lifting motor is installed on the slider assembly II, the output end of the condenser lifting motor is connected with the ball screw II, the ball screw II is threadedly connected with a slider assembly III that is slidably connected to the guide rail II, the slider assembly II is connected to the stage through the stage connecting rod, the slider assembly III is connected to the condenser assembly through the condenser connecting rod, and flexible connecting parts are provided between the slider assembly II and the stage connecting rod, and between the slider assembly III and the condenser connecting rod.

[0009] As a further improvement, the output end of the main lifting motor is connected to the ball screw I through a coupling, and a screw fixing seat is also provided on the other side of the supporting vertical plate. The end of the ball screw I away from the main lifting motor is rotatably mounted on the screw fixing seat through a bearing.

[0010] Furthermore, sliding holes are provided on the supporting upright plates corresponding to the stage connecting rod and the condenser connecting rod, and a limiting assembly is provided above the sliding holes corresponding to the stage connecting rod.

[0011] Furthermore, the limit assembly includes a limit block, an adjustment seat and an adjustment screw. The support vertical plate is provided with a limit rod, the limit block sliding sleeve is provided on the limit rod, the adjustment seat is fixed on the support vertical plate, the adjustment screw is threadedly connected to the adjustment seat, and the adjustment screw is rotatably connected to the limit block through a bearing, and a flexible connecting piece is provided on the side of the limit block close to the stage connecting rod.

[0012] Furthermore, the light source assemblies and the objective lens eyepiece assemblies on both sides of the guide rail I are threadedly connected with reverse screws.

[0013] Furthermore, the worktable includes an upper support plate, a lower support plate and a pressure plate assembly, the lower support plate is connected to the worktable connecting rod and to the slider on the guide rail I, the upper support plate is mounted on the lower support plate for sliding back and forth, the pressure plate assembly is mounted on the upper support plate for sliding left and right, the lower support plate is provided with a first screw rod and nut pair, the output end of the first screw rod and nut pair is connected to the upper support plate, the pressure plate assembly is provided with a second screw rod and nut pair, the output end of the second screw rod and nut pair is connected to the upper support plate.

[0014] Furthermore, a spring II is provided between the output end of the first screw nut pair and the lower support plate, and between the pressing plate assembly and the upper support plate.

[0015] In order to achieve the above-mentioned second objective, the present invention provides a method for controlling an electric microscope, including the above-mentioned electric microscope, the method including resetting the microscope system and observing the specimen:

[0016] The microscope system resetting comprises the following steps:

[0017] S1: Control the condenser lifting motor to raise the condenser assembly to a distance of 1-2 mm from the stage surface;

[0018] S2: Control the main lifting motor to lower the stage to 2-4mm from its lower limit;

[0019] S3: Control the first screw nut pair and the second screw nut pair to work, and drive the pressing assembly to move to the zero position of the X and Y axes;

[0020] The steps for observing the specimen include:

[0021] After the microscope system reset step is completed, place the specimen slide on the stage and fix it with the slide pressing assembly. By controlling the first screw nut pair, the second screw nut pair, the main lifting motor, and the condenser lifting motor, the specimen slide is placed near the microscope field of view to make the observation target clear.

[0022] Beneficial Effects

[0023] Compared with the prior art, the present invention has the following advantages:

[0024] The electric microscope of the present invention drives the stage and the condenser assembly to rise and fall through an electric mechanism, and adjusts the position of the specimen slide through a screw nut pair, which can increase the forward and backward and left and right movement range of the specimen slide. At the same time, through the provision of springs and flexible connectors, the risk of electric control breaking the specimen slide or damaging the mechanical structure is reduced, and the impact of shaking during the stage lifting process is reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a structural schematic diagram of the front side of the present invention;

[0026] Figure 2 It is a structural schematic diagram of the back side of the present invention;

[0027] Figure 3 for Figure 2 A schematic diagram of the structure enlargement at the center A;

[0028] Figure 4 for Figure 2 A schematic diagram of the structure enlarged at B in the middle;

[0029] Figure 5 It is a schematic diagram of the enlarged structure of the object carrier in the present invention.

[0030] Among them: 1-base, 2-support plate, 3-guide rail I, 4-light source assembly, 5-condenser assembly, 6-stage, 7-main lifting motor, 8-guide rail II, 9-ball screw I, 10-slider assembly I, 11-pressure adjustment screw, 12-spring I, 13-slider assembly II, 14-condenser lifting motor, 15-ball screw II, 16-slider assembly III, 17-stage connecting rod, 18-condenser Connecting rod, 19-flexible connector, 20-coupling, 21-screw fixing seat, 22-slide hole, 23-limit block, 24-adjusting seat, 25-adjusting screw, 26-limit rod, 27-objective lens eyepiece assembly, 28-reverse top screw, 61-upper support plate, 62-lower support plate, 63-pressing plate assembly, 64-first screw nut pair, 65-second screw nut pair, 66-spring II, 631-positioning plate, 632-pressing plate. DETAILED DESCRIPTION

[0031] The present invention will be further described below with reference to the specific embodiments in the accompanying drawings.

[0032] See also Figure 1-5As shown, an electric microscope of the present invention comprises a base 1 and a supporting upright plate 2, wherein the supporting upright plate 2 is vertically fixed on the surface of the base 1, and a guide rail 13 is fixed on one side of the supporting upright plate 2, wherein the guide rail 13 is a track with grooves on both sides, and four sliders are slidably mounted on the guide rail 13, and the four sliders are all U-shaped sliders with protrusions on the inner side walls, and the protrusions are slidably inserted in the grooves to prevent the sliders from detaching from the guide rail 1, and the four sliders are distributed from bottom to top and are respectively fixed with a light source assembly 4, a condenser assembly 5, a stage 6 and an objective lens eyepiece assembly 27, and the light source assembly 4, the condenser assembly 5 and the objective lens eyepiece assembly 27 are all structures in the prior art and will not be described herein. On the other side of the supporting vertical plate 2, there is an electric mechanism for controlling the lifting and lowering of the condenser assembly 5 and the stage 6. Specifically, the electric mechanism includes a main lifting motor 7, a guide rail II8, a ball screw I9 and a slider assembly I10, wherein the main lifting motor 7 and the guide rail II8 are fixed on the supporting vertical plate 2, and the output end of the main lifting motor 7 is connected to the ball screw I9, the slider assembly I10 is slidably installed on the guide rail II8, and the slider assembly I10 is threadedly connected to the ball screw I9. The main lifting motor 7 drives the ball screw I9 to rotate when it works, and then drives the slider assembly I10 to rise and fall. A pressure adjustment screw 11 is threadedly connected to the slider assembly I10, and a spring I12 is sleeved on the pressure adjustment screw 11 on one side of the spring I12. A slider assembly II13 is provided which is slidably connected to the guide rail II8, and the two ends of the spring I12 are respectively connected to the slider assembly I10 and the slider assembly II13. A condenser lifting motor 14 is installed on the slider assembly II13. The output end of the condenser lifting motor 14 is connected to a ball screw II15, and a slider assembly III16 which is slidably connected to the guide rail II8 is threadedly connected to the ball screw II15. The condenser lifting motor 14 drives the ball screw II15 to rotate, thereby driving the slider assembly III16 to move up and down. The slider assembly II13 is connected to the stage 6 through the stage connecting rod 17 to drive the stage 6 to move up and down. The slider assembly III16 is connected to the condenser assembly 5 through the condenser connecting rod 18 to drive the condenser assembly 5 to move up and down. The main lifting motor 7 and the condenser lifting motor 14 are adopted to realize the condenser assembly 5 to move up and down independently driven by the condenser lifting motor 14, and the condenser assembly 5 and the stage 6 can also be driven to move simultaneously by the main lifting motor 7, which is more convenient for adjustment. A flexible connector 19 is provided between the slider assembly II15 and the stage connecting rod 17, and between the slider assembly III16 and the condenser connecting rod 18. The flexible connector 19 can be a rubber pad or a plastic pad to play a role of vibration reduction and buffering. The electric microscope also includes a controller, and the main lifting motor 7 and the condenser lifting motor 14 are electrically connected to the controller. Specifically, the main lifting motor 7, the condenser lifting motor 14 and the controller can be connected by wires for easy control.

[0033] The electric microscope of the present invention drives the stage and the condenser assembly to rise and fall through an electric mechanism, and adjusts the position of the specimen slide through a screw nut pair, which can increase the forward and backward and left and right movement range of the specimen slide. At the same time, through the provision of springs and flexible connectors, the risk of electric control breaking the specimen slide or damaging the mechanical structure is reduced, and the impact of shaking during the stage lifting process is reduced.

[0034] Preferably, the output end of the main lifting motor 7 is connected to the ball screw I9 through a coupling 20, and a screw fixing seat 21 is also provided on the other side of the supporting vertical plate 2. The end of the ball screw I9 away from the main lifting motor 7 is rotatably mounted on the screw fixing seat 21 through a bearing, thereby further improving the transmission stability.

[0035] Preferably, sliding holes 22 are provided on the supporting uprights 2 corresponding to the stage connecting rod 17 and the condenser connecting rod 18. The stage connecting rod 17 and the condenser connecting rod 18 pass through the sliding holes 22 to avoid interference between the stage connecting rod 17 and the condenser connecting rod 18 and the supporting uprights 2. A limit assembly is provided above the sliding hole 22 corresponding to the stage connecting rod 17 to limit the rising stroke of the stage 6. Specifically, the limit assembly includes a limit block 23, an adjustment seat 24 and an adjustment screw 25. The support plate 2 is provided with a limit rod 26. The limit block 23 is slidably sleeved on the limit rod 26. The adjustment seat 24 is fixed on the support plate 2. The adjustment screw 25 is threadedly connected to the adjustment seat 24, and the adjustment screw 25 is rotatably connected to the limit block 23 through a bearing. The limit block 23 is provided with a flexible connector 19 on the side close to the stage connecting rod 17. The flexible connector 19 can be a rubber pad or a plastic pad, which plays a role in vibration reduction and buffering to avoid rigid collision. During use, the limit block 23 can be driven to move by rotating the adjustment screw 25, thereby adjusting the size of the ascending stroke of the stage 6.

[0036] Preferably, the light source assembly 4 and the objective lens eyepiece assembly 27 on both sides of the guide rail 13 are threadedly connected with a reverse top screw 28. In actual use, the reverse top screw 28 can be loosened, and then the position of the light source assembly 4 and the objective lens eyepiece assembly 27 can be adjusted, and finally the reverse top screw 28 can be tightened to adjust the position of the light source assembly 4 and the objective lens eyepiece assembly 27 to adapt to the specifications of different objective lenses, which is more convenient to use.

[0037] Preferably, the stage 6 includes an upper support plate 61, a lower support plate 62 and a tablet pressing assembly 63, wherein the lower support plate 61 is connected to the stage connecting rod 17 and is connected to the slider on the guide rail 13, the upper support plate 61 is mounted on the lower support plate 62 to slide forward and backward, and the tablet pressing assembly 63 is mounted on the upper support plate 61 to slide left and right to achieve forward and backward, left and right movement and adjustment of the specimen slide, a first screw nut pair 64 is provided on the lower support plate 62, and the output end of the first screw nut pair 64 is connected to the upper support plate 61, and a second screw nut pair 65 is provided on the tablet pressing assembly 63, and the output end of the second screw nut pair 65 is connected to the upper support plate 61 to achieve electric control of the movement and adjustment of the specimen slide. Specifically, the tablet pressing assembly 63 includes a positioning plate 631 and a clamping plate 632, and the clamping plate 632 is rotatably mounted on the positioning plate 631. The first screw nut pair 64 and the second screw nut pair 65 are both electrically connected to the controller for easy control.

[0038] Preferably, a spring II66 is provided between the output end of the first screw nut pair 64 and the lower support plate 62, and between the pressing plate assembly 63 and the upper support plate 61, which also plays a buffering role.

[0039] A method for controlling an electric microscope, comprising the electric microscope described above, the method comprising resetting the microscope system and observing a specimen:

[0040] The microscope system resetting comprises the following steps:

[0041] S1: Control the condenser lifting motor 14 to work, and raise the condenser assembly 5 to a distance of 1-2 mm from the surface of the stage 6; specifically, when the condenser lifting motor 14 is working, the condenser assembly 5 is raised to the detection position of the photoelectric gate I, and this position is set as the zero point of the condenser assembly 5. The detection position of the photoelectric gate I is preferably at a distance of 1-2 mm from the top of the condenser assembly 5 to the surface of the stage 6. The photoelectric gate is disconnected when the condenser assembly 5 is above the zero position; when the condenser assembly 5 is below the zero position, the photoelectric gate is turned on, and the number of steps of the stepper motor at the upper and lower limits is set according to different condensers through the software. Whenever the condenser assembly 5 rises and passes through the photoelectric gate, the motor step count is calibrated and cleared to eliminate the accumulated error. This reduces the risk of the condenser damaging the glass slide, and can adapt to condensers of different specifications;

[0042] S2: Control the main lifting motor 7 to work, and lower the stage 6 to 2-4 mm from its lower limit position; specifically, when the main lifting motor 7 is working, the stage 6 is lowered to the photoelectric gate II detection position, and this position is set as the zero point of the stage 6. The photoelectric gate II detection position is preferably 2-4 mm away from the lower limit position of the stage 6, which is convenient for installing and replacing the objective lens, and at the same time, the lower limit space is reserved, and there is no need to accurately adjust the sensor position;

[0043] S3: Control the first screw nut pair 64 and the second screw nut pair 65 to work, and drive the pressing plate assembly 63 to move to the zero position of the X and Y axes; specifically, calibrate the positions of the X and Y axes while executing the above steps, set the detection position of the X-axis photoelectric gate III as the zero point of the X axis, and set the detection position of the Y-axis photoelectric gate IIII as the zero point of the Y axis. The detection position of the photoelectric gate III is preferably near the position where the X axis comes out, and a reflective photoelectric gate is used for easy installation. The detection position of the photoelectric gate IIII is preferably near the position closest to the inside of the Y axis, and a reflective photoelectric gate is used for easy installation.

[0044] The steps for observing the specimen include:

[0045] After the microscope system reset step is completed, it can be used normally. Place the specimen slide on the stage 6 and fix it with the tablet pressing assembly 63. Specifically, place the slide on the upper support plate 61 of the stage 6 so that the two adjacent sides of the slide are respectively attached to the inner side of the positioning plate 631 corresponding to the tablet pressing assembly 63. Turn the pressing plate 632 corresponding to the tablet pressing assembly 63 to move to one side of the positioning plate 631 until the pressing plate 632 is attached to the other side of the slide to complete the fixation of the slide. The software calls out the preset observation position parameters to control the first screw nut pair 64, the second screw nut pair 65, the main lifting motor 7, and the condenser lifting motor 14 to make the specimen slide near the microscope field of view. Then, fine-tune the positions of each motor and other components according to the observation situation to make the observation target clear. The purpose of rapid observation can be achieved through the preset parameter setting. If combined with the machine vision system, the entire adjustment process can be automated.

[0046] The electric microscope control method can realize rapid detection of specimens.

[0047] The above are only preferred embodiments of the present invention. It should be pointed out that for those skilled in the art, several modifications and improvements can be made without departing from the structure of the present invention, which will not affect the effect of the implementation of the present invention and the practicality of the patent.

Claims

1. An electric microscope, characterized in that: The invention comprises a base (1) and a supporting plate (2), wherein the supporting plate (2) is vertically fixed on the surface of the base (1), a guide rail I (3) is fixed on one side of the supporting plate (2), four sliders are slidably mounted on the guide rail I (3), and the four sliders are distributed from bottom to top and are respectively fixed with a light source assembly (4), a condenser assembly (5), a stage (6) and an objective lens and eyepiece assembly (27); the other side of the supporting plate (2) is provided with a control mechanism for controlling the lifting of the condenser assembly (5) and the stage (6); The electric mechanism comprises a main lifting motor (7), a guide rail II (8), a ball screw I (9) and a slider assembly I (10), wherein the main lifting motor (7) and the guide rail II (8) are both fixed on the supporting vertical plate (2), and the output end of the main lifting motor (7) is connected to the ball screw I (9), the slider assembly I (10) is slidably mounted on the guide rail II (8), and the slider assembly I (10) is threadedly connected to the ball screw I (9), and the slider assembly A pressure adjustment screw (11) is threadedly connected to the guide rail I (10), and a spring I (12) is sleeved on the pressure adjustment screw (11). A slider assembly II (13) slidably connected to the guide rail II (8) is sleeved on the pressure adjustment screw (11) on one side of the spring I (12). A condenser lifting motor (14) is installed on the slider assembly II (13). The output end of the condenser lifting motor (14) is connected to a ball screw II (15). The ball screw II (15) is connected to the output end of the ball screw II (15). 5) is threadedly connected to a slider assembly III (16) which is slidably connected to the guide rail II (8), the slider assembly II (13) is connected to the stage (6) through a stage connecting rod (17), the slider assembly III (16) is connected to the condenser assembly (5) through a condenser connecting rod (18), and flexible connecting parts (19) are provided between the slider assembly II (15) and the stage connecting rod (17), and between the slider assembly III (16) and the condenser connecting rod (18).

2. An electric microscope according to claim 1, characterized in that: The output end of the main lifting motor (7) is connected to the ball screw I (9) via a coupling (20), and a screw fixing seat (21) is also provided on the other side of the supporting vertical plate (2), and the end of the ball screw I (9) away from the main lifting motor (7) is rotatably mounted on the screw fixing seat (21) via a bearing.

3. An electric microscope according to claim 1, characterized in that: The supporting upright plates (2) corresponding to the stage connecting rod (17) and the condenser connecting rod (18) are both provided with sliding holes (22), and a limiting component is provided above the sliding hole (22) corresponding to the stage connecting rod (17).

4. An electric microscope according to claim 3, characterized in that: The limit assembly comprises a limit block (23), an adjustment seat (24) and an adjustment screw (25); a limit rod (26) is provided on the support vertical plate (2); the limit block (23) is slidably sleeved on the limit rod (26); the adjustment seat (24) is fixed on the support vertical plate (2); the adjustment screw (25) is threadedly connected to the adjustment seat (24); and the adjustment screw (25) is rotatably connected to the limit block (23) via a bearing; and a flexible connector (19) is provided on the side of the limit block (23) close to the stage connecting rod (17).

5. The electric microscope according to claim 1, characterized in that: The light source assembly (4) and the objective lens eyepiece assembly (27) on both sides of the guide rail 1 (3) are both threadedly connected with reverse screws (28).

6. An electric microscope according to any one of claims 1 to 5, characterized in that: The loading platform (6) comprises an upper support plate (61), a lower support plate (62) and a pressing plate assembly (63); the lower support plate (61) is connected to the loading platform connecting rod (17) and to a slider on the guide rail I (3); the upper support plate (61) is mounted on the lower support plate (62) so as to slide forward and backward; the pressing plate assembly (63) is mounted on the upper support plate (61) so as to slide left and right; a first screw nut pair (64) is provided on the lower support plate (62); an output end of the first screw nut pair (64) is connected to the upper support plate (61); a second screw nut pair (65) is provided on the pressing plate assembly (63); an output end of the second screw nut pair (65) is connected to the upper support plate (61).

7. An electric microscope according to claim 6, characterized in that: A spring II (66) is provided between the output end of the first screw nut pair (64) and the lower support plate (62), and between the pressing plate assembly (63) and the upper support plate (61).

8. A method for controlling an electric microscope, characterized in that: The method comprises resetting the microscope system and observing the specimen, comprising: The microscope system resetting comprises the following steps: S1: Control the condenser lifting motor (14) to work, and raise the condenser assembly (5) to a distance of 1-2 mm from the surface of the stage (6); S2: Control the main lifting motor (7) to work, and lower the loading platform (6) to 2-4 mm from its lower limit position; S3: Control the first screw nut pair (64) and the second screw nut pair (65) to work, driving the pressing assembly (63) to move to the zero position of the X and Y axes; The steps for observing the specimen include: After the microscope system reset step is completed, the specimen slide is placed on the stage (6) and fixed with a pressing assembly (63). By controlling the first screw nut pair (64), the second screw nut pair (65), the main lifting motor (7), and the condenser lifting motor (14), the specimen slide is placed near the microscope field of view, so that the observation target is clear.

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