Method for promoting postpartum reproductive performance and lactation performance

Abstract

The invention provides a method for improving the postpartum reproductive performance and / or lactation performance of a mammal. The method comprises the step of applying alpha-ketoglutaric acid to the mammal in the perinatal period. According to the present invention, the alpha-ketoglutaric acid is added to the perinatal mammal feed so as to improve the energy metabolism, the immune function and the lactation expression, such that the innovative solution is provided for solving the perinatal metabolism challenge of the alpha-ketoglutaric acid.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for promoting postpartum reproductive performance and / or lactation performance. Background Art

[0002] The peripartum period of dairy cows refers to the critical physiological stage from 21 days before to 21 days after parturition. During this stage, dairy cows not only need to support the rapid growth of the fetus, but also need to start the secretion of colostrum, resulting in a sharp increase in nutritional needs. However, due to the fetus's occupation of abdominal space and endocrine changes, the dry matter intake of cows is significantly reduced, thereby reducing the energy intake level. This puts dairy cows into a state of negative energy balance (NEB). In order to compensate for this state, dairy cows rely on body fat mobilization. However, excessive body fat mobilization may bring many negative effects, such as increasing the metabolic burden on the liver, causing oxidative stress, reducing immune function, and increasing the risk of metabolic diseases. Therefore, nutritional management during the peripartum period of dairy cows is particularly important. Summary of the invention

[0003] The present disclosure provides a method for improving the postpartum reproductive performance and / or lactation performance of mammals. The present disclosure provides an innovative solution to the metabolic challenge of α-ketoglutarate during the peripartum period by adding α-ketoglutarate to the feed of peripartum mammals to improve their energy metabolism, immune function and lactation performance.

[0004] According to one aspect of the present disclosure, there is provided use of α-ketoglutarate in improving postpartum reproductive performance and / or lactation performance of mammals.

[0005] In some embodiments, the alpha-ketoglutarate is used to enhance anti-inflammatory properties in a mammal.

[0006] In some embodiments, the α-ketoglutarate is used to enhance antioxidant capacity in a mammal.

[0007] In some embodiments, the alpha-ketoglutarate is used to reduce the incidence of postpartum diseases in a mammal.

[0008] In some embodiments, the α-ketoglutarate is used to improve the breeding pregnancy rate and shorten the postpartum mating days of mammals.

[0009] In some embodiments, the α-ketoglutarate is used to increase the milk fat and / or milk protein content of postpartum colostrum of a mammal.

[0010] In some embodiments, the α-ketoglutarate is used to reduce the lactose and / or urea nitrogen content of postpartum colostrum of a mammal.

[0011] In some embodiments, the α-ketoglutarate is used to increase the daily milk production of a mammal in the early lactation period.

[0012] In some embodiments, the α-ketoglutarate is used to reduce somatic cell count in mammalian milk.

[0013] In some embodiments, the alpha-ketoglutarate is used to increase the birth weight of a newborn mammal.

[0014] In some embodiments, the disease includes, but is not limited to, one or more of retained placenta, metritis, mastitis, postpartum paralysis, ketosis, and diarrhea.

[0015] In some embodiments, the mammal comprises livestock.

[0016] In some embodiments, the mammal comprises a pig, a cow, a sheep, a horse, a donkey, or a rabbit.

[0017] In some embodiments, the mammal is a cow.

[0018] According to a second aspect of the present disclosure, a method for improving postpartum reproductive performance and / or lactation performance of a mammal is provided, the method comprising the step of administering α-ketoglutarate to a peripartum mammal.

[0019] In some embodiments, the alpha-ketoglutarate is administered by feeding.

[0020] In some embodiments, the amount of α-ketoglutarate is 0.5-20 g / day.

[0021] In some embodiments, the amount of α-ketoglutarate is 1-10 g / day.

[0022] In some embodiments, the amount of α-ketoglutarate is 5-10 g / day.

[0023] In some embodiments, the method comprises feeding alpha-ketoglutarate to the mammal during the peripartum period and stopping feeding after the mammal has given birth.

[0024] In some embodiments, alpha-ketoglutarate is fed to the mammal for 10-16 days prepartum, and feeding is stopped after the mammal gives birth.

[0025] In some embodiments, the mammal comprises livestock.

[0026] In some embodiments, the mammal comprises a pig, a cow, a sheep, a horse, a donkey, or a rabbit.

[0027] In some embodiments, the mammal is a cow.

[0028] In some embodiments, the improving the postpartum reproductive performance and / or lactation performance of a mammal comprises at least one of the following:

[0029] (1) Improve the anti-inflammatory properties of mammals;

[0030] (2) Improve the antioxidant capacity of mammals;

[0031] (3) Improve protein metabolism and energy metabolism in periparturient dairy cows;

[0032] (4) reduce the incidence of postpartum diseases in mammals;

[0033] (5) Improve the postpartum pregnancy rate of mammals and shorten the number of days after mating;

[0034] (6) Improve the ability of mammals to initiate lactation after childbirth;

[0035] (7) increasing the milk fat and / or milk protein content of postpartum colostrum of mammals;

[0036] (8) reducing the lactose and / or urea nitrogen content of postpartum colostrum of mammals;

[0037] (9) Increase the daily milk production of mammals in the early lactation period;

[0038] (10) Reducing the number of somatic cells in mammalian milk;

[0039] (11) Increasing the birth weight of newborn mammals;

[0040] (12) Reduce the incidence of diseases in newborn mammals;

[0041] (13) Improve the survival rate of newborn mammals.

[0042] In some embodiments, the disease includes, but is not limited to, one or more of retained placenta, metritis, mastitis, postpartum paralysis, ketosis, and diarrhea.

[0043] The method disclosed in the present invention is mainly applicable to the feeding and management of peripartum mammals, especially peripartum dairy cows, and provides an effective solution to the problem of negative energy balance in dairy cows during the peripartum period, helping dairy cows to smoothly pass this critical period. In actual production, supplementation of α-ketoglutaric acid (AKG) can significantly improve the health level and reproductive performance of peripartum dairy cows, reduce the incidence of diseases, and reduce economic losses. At the same time, it improves the lactation performance of dairy cows, increases milk production, and thus improves the economic benefits of farms and promotes the sustainable development of animal husbandry.

[0044] The present disclosure unexpectedly found that feeding α-ketoglutarate in the early peripartum period not only significantly increased the daily milk production of dairy cows, but also improved the quality of milk components. In particular, in the colostrum stage, the addition of α-ketoglutarate has an important regulatory effect on key indicators such as milk fat, milk protein, lactose, urea nitrogen and somatic cell count. This is specifically reflected in the following aspects:

[0045] (1) Improving colostrum quality: Colostrum is a key source of immunity and nutrients for newborn calves in their early life. The increase in the content of milk fat and milk protein in colostrum means that the colostrum is of better quality, which can provide calves with more energy and essential nutrients, enhance the immunity and growth and development of newborn calves, help reduce the incidence of calf diseases, and increase the survival rate and birth weight of calves.

[0046] (2) Enhance the smoothness of the start of lactation in dairy cows: High milk fat and milk protein content indicates that the mammary gland function of dairy cows is good at the start of lactation and has a good foundation for lactation performance. Although the milk fat rate and milk protein rate tend to stabilize in the later stage, good colostrum performance may lay the foundation for a smooth transition in the early stage of lactation and future milk production.

[0047] (3) Improved economic benefits: For farms, improved colostrum quality can help reduce early calf mortality and disease treatment costs, thereby improving overall economic benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 The dynamic changes of blood metabolism and liver function related indicators of dairy cows are shown. (A) Non-esterified fatty acids (NEFA), (B) β-hydroxybutyric acid (BHBA), (C) alanine aminotransferase (ALT), (D) aspartate aminotransferase (AST).

[0049] Figure 2 Dynamic changes of serum biochemical indices in dairy cows. (A) Serum total protein (TP), (B) albumin (ALB), (C) glucose (GLU), (D) triglyceride (TG), (E) total cholesterol (TC), (F) urea nitrogen (BUN).

[0050] Figure 3 Dynamic changes of serum immune and antioxidant levels. (A) Interleukin 1β (IL-1β), (B) Interleukin 6 (IL-6), (C) Interleukin 10 (IL-10), (D) Tumor necrosis factor α (TNF-α), (E) Immunoglobulin A (IgA). (F) Total antioxidant capacity (T-AOC), (G) Superoxide dismutase (SOD), (H) Malondialdehyde (MDA), (I) Glutathione peroxidase (GSH-PX).

[0051] Figure 4Dynamic changes of serum reproductive hormones. (A) estradiol (E2), (B) progesterone (P4), (C) prolactin (PRL), (D) cortisol (COR).

[0052] Figure 5 Effects of calf birth weight and postpartum registration days. (A) calf birth weight, (B) postpartum registration days.

[0053] Figure 6 Changes in milk composition and daily milk production. (A) Milk fat percentage, (B) Milk protein percentage, (C) Lactose percentage, (D) Urea nitrogen, (E) Somatic cell count, (F) Daily milk production.

[0054] Figure 1-6 In the table, “*”, “**”, “***” and “****” indicate significant differences among the groups and “P<0.05”, “P<0.01”, “P<0.001” and “P<0.0001”, respectively. DETAILED DESCRIPTION

[0055] α-Ketoglutarate (AKG) is an important intermediate in the tricarboxylic acid cycle, widely involved in energy metabolism, and plays a core role in regulating immune function and anti-oxidation. AKG can not only serve as a key regulatory factor in energy metabolism, but also improve the energy utilization efficiency of dairy cows, reduce oxidative stress, and enhance the immune system function, thereby reducing the risk of metabolic diseases in dairy cows during the peripartum period.

[0056] The present invention improves the postpartum reproductive performance and lactation performance of dairy cows by feeding α-ketoglutarate to dairy cows in the peripartum period, specifically, adding α-ketoglutarate to the TMR diet of dairy cows in the early peripartum period. The inventor unexpectedly found that supplementing α-ketoglutarate in the early peripartum period can improve the anti-inflammatory and antioxidant properties of dairy cows, reduce the incidence of postpartum diseases in dairy cows, increase the breeding pregnancy rate of dairy cows after delivery and shorten the number of postpartum mating days, increase the milk fat and milk protein content of colostrum after delivery of dairy cows, increase the daily milk production in the early lactation period and reduce the number of somatic cells in milk.

[0057] In order to make the purpose, technical scheme and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments and drawings. The specific embodiments described herein are only used to explain the present invention and are not intended to constitute any limitation to the present invention. In addition, in the following description, the description of known structures and technologies is omitted to avoid unnecessary confusion of the concepts of the present disclosure. Such structures and technologies are also described in many publications.

[0058] The reagents and materials used in the following examples are all commercially available products. Among them, the total mixed ration (TMR) for dairy cows includes 3.5wt% corn steam flakes, 2.2wt% corn husk spray, 2.2wt% low-fat DDGS, 4.4wt% cereal straw, 13.2wt% oats, 57wt% corn silage, and 17.5wt% Shounong dry 20210410.

[0059] Example 1

[0060] A total of 180 healthy multiparous dairy cows about to enter the peripartum period (21 days before delivery to 21 days after delivery) with basically the same body condition were selected and randomly divided into 4 groups, with 45 cows in each group. Three of the groups added α-ketoglutaric acid (AKG) to the total mixed ration (TMR) mixer of dairy cows at noon every day, and the amount of addition was such that each dairy cow took in an average of 1g, 5g and 10g α-ketoglutaric acid per day, namely 1g AKG, 5g AKG and 10g AKG in the experimental group. α-Ketoglutaric acid was added from the 14th day before delivery to the dairy cows and stopped after delivery. At the same time, the control group (con) was fed only with the total mixed ration. In the pre-perinatal period, blood samples were collected once 14 days before delivery and 7 days before delivery for subsequent testing; in the late peripartum period, milk and blood samples were collected once within 2 hours of delivery and 4, 7 and 14 days after delivery for subsequent testing.

[0061] The results are as follows:

[0062] 1. Effects of feeding α-ketoglutarate during the peripartum period on serum metabolism and liver function-related indicators in dairy cows

[0063] Serum non-esterified fatty acids (NEFA, Figure 1 A), beta-hydroxybutyric acid (BHBA, Figure 1 B), alanine aminotransferase (ALT, Figure 1 C) and aspartate aminotransferase (AST, Figure 1 The results showed that when dairy cows were fed α-ketoglutarate in the early peripartum period, although the indicators related to metabolism and liver function showed certain fluctuations in the peripartum period, no significant differences were observed among the groups (P>0.05).

[0064] The above results show that feeding α-ketoglutarate in the early peripartum period has no obvious negative impact on the metabolic and liver function indicators of dairy cows, indicating that it is safe and will not interfere with the normal metabolic function of dairy cows.

[0065] 2. Effects of feeding α-ketoglutarate on serum biochemical parameters of dairy cows during the pre-peripartum period

[0066] On the day of delivery, the serum total protein (TP) levels of the 5g AKG group and the 10g AKG group were significantly higher than those of the control group (P<0.05, Figure 2 A).

[0067] The change trend of albumin (ALB) was consistent between the experimental groups and the control group, but the difference was not significant (P>0.05, Figure 2 B).

[0068] The trends of serum glucose (GLU) in each group were basically the same. The GLU levels in the 5g AKG group and the 10g AKG group were significantly higher than those in the control group on the day of delivery (P<0.05, Figure 2 C), and the 10g AKG group was higher than the control group at other time points but not significantly higher.

[0069] The levels of serum triglyceride (TG) in dairy cows in the experimental groups and the control group tended to be consistent, reaching the highest level 7 days before delivery and then decreasing. In addition, 7 days before delivery, the TG in the 5g AKG group and the 10g AKG group was significantly lower than that in the 1g AKG group (P<0.05, Figure 2 D).

[0070] There was no significant difference in the changes of serum total cholesterol (TC) among the groups (P>0.05, Figure 2 E).

[0071] In terms of serum urea nitrogen (BUN) levels, 7 days before delivery, the BUN level of the 1g AKG group was significantly higher than that of the 5g AKG group (P<0.05); 7 days after delivery, the BUN levels of the 5g AKG group and the 10g AKG group were significantly lower than those of the control group (P<0.05, Figure 2 F).

[0072] The above results show that feeding higher doses of α-ketoglutarate can significantly improve the protein metabolism and energy metabolism of periparturient dairy cows, help maintain higher levels of total protein and glucose in their serum, and help dairy cows better cope with the metabolic challenges of the periparturient period.

[0073] 3. Effects of feeding α-ketoglutarate on serum immunity and antioxidant properties of dairy cows during the peripartum period

[0074] After feeding different doses of AKG, the serum immune indexes of dairy cows in each experimental group showed certain changes.

[0075] Interleukin 1β (IL-1β, Figure 3 A) and interleukin-6 (IL-6, Figure 3 B) The overall level in the experimental group was lower than that in the control group.

[0076] The levels of interleukin 10 (IL-10) in the 5g AKG group and the 10g AKG group were significantly higher than those in the control group on day 4 after delivery (P<0.05, Figure 3 C), and 7 days after delivery, the IL-10 level in the 10g AKG group was still significantly higher than that in the control group (P<0.05).

[0077] Tumor necrosis factor α (TNF-α, Figure 3 D) In ​​each experimental group, the TNF-α level was significantly lower than that in the control group, among which the TNF-α level in the 5g AKG group and the 10g AKG group was significantly lower than that in the control group 4 days after delivery (P<0.05).

[0078] Immunoglobulin A (IgA, Figure 3 E) The changing trends were consistent among the groups, and the IgA level in the 10g AKG group was generally higher than that in the other groups.

[0079] Serum total antioxidant capacity (T-AOC, Figure 3 F), superoxide dismutase (SOD, Figure 3 G) and malondialdehyde (MDA, Figure 3 H) had a similar trend and was higher than that in the control group.

[0080] However, glutathione peroxidase (GSH-PX, Figure 3 The level of GSH-PX in the experimental group was higher than that in the control group, especially at 7 days after delivery, the level of GSH-PX in the 10g AKG group was significantly higher than that in the control group (P<0.05).

[0081] The above results indicate that α-ketoglutarate can improve the immune function of periparturient dairy cows and reduce their risk of inflammatory diseases by enhancing their antioxidant capacity and reducing inflammatory response.

[0082] 4. Effect of feeding α-ketoglutarate during the peripartum period on reproductive performance of dairy cows

[0083] 4.1 Reproductive hormones

[0084] Serum estradiol (E2, Figure 4 A) There were significant differences in the levels. At 7 days before delivery, 7 days after delivery, and 14 days after delivery, the E2 levels in the 5g AKG group and the 10g AKG group were significantly higher than those in the control group (P<0.05).

[0085] Serum progesterone (P4, Figure 4 The change trend of P4 in the experimental group and the control group was consistent. The P4 level was maintained at a high value before delivery, and then dropped rapidly and remained stable after delivery, which was in line with the physiological law of dairy cows. There was no significant difference in the P4 level between the experimental group and the control group (P>0.05).

[0086] Prolactin (PRL, Figure 4 C) At 7 days before delivery, the PRL level in the 1g AKG group was significantly higher than that in the 5g AKG group (P<0.05), and was also higher than that in the control group.

[0087] Serum cortisol (COR, Figure 4 D) The level changes showed that the COR level in the 10 g AKG group was generally lower than that in the other groups.

[0088] The above results show that prolactin is an important regulatory hormone for lactation. Its increase can promote the development of mammary glands and the synthesis of milk protein and milk fat, thereby increasing milk production and milk quality. Serum cortisol is an important marker of stress response. Reducing COR indicates that the stress level of dairy cows is reduced, which helps to maintain normal metabolism and production performance. It can be seen that feeding α-ketoglutaric acid in the early peripartum period can improve the reproductive performance of dairy cows after delivery, significantly shorten the number of days after delivery, and increase the pregnancy rate of dairy cows in the first and second matings.

[0089] 4.2 Postpartum breeding pregnancy rate and disease incidence

[0090] The addition of AKG had a positive effect on the first mating pregnancy rate and the cumulative pregnancy rate of two matings in dairy cows after calving. In particular, the first mating pregnancy rate and the cumulative pregnancy rate of two matings in the 5g AKG group and the 10g AKG group were significantly higher than those in the control group (P<0.05, Table 1).

[0091] Table 1 Postpartum pregnancy rate of dairy cows fed with α-ketoglutarate

[0092]

[0093]

[0094] Note: Different lowercase letters in the same column indicate significant differences (P<0.05), while the same letters indicate insignificant differences (P>0.05).

[0095] In addition, the incidence of postpartum diseases in the experimental group was lower than that in the control group, especially in the 5g AKG and 10g AKG groups, with an incidence of 26.78%, which was significantly lower than that in the control group (P<0.05, Table 2).

[0096] Table 2 Occurrence of postpartum diseases in dairy cows after feeding α-ketoglutarate

[0097]

[0098] Note: Different lowercase letters in the same column indicate significant differences (P<0.05), while the same letters indicate insignificant differences (P>0.05).

[0099] 4.3 Calf birth weight and dairy cow postpartum registration days

[0100] The birth weight of calves in the experimental group fed with AKG was higher than that in the control group, especially the birth weight of calves in the group fed with 10g AKG was significantly higher than that in the control group ( Figure 5 A).

[0101] The statistical results of the mating days (the mating days refer to the number of days from the cow's calving to the last successful mating) showed that the mating days of the cows in the experimental group were less than those in the control group, especially the mating days of the cows in the 5g AKG group and the 10g AKG group were significantly less than those in the control group ( Figure 5 B).

[0102] 5. Effects of feeding α-ketoglutarate during the peripartum period on daily milk production and milk composition of dairy cows

[0103] Milk fat percentage ( Figure 6 A). Within 2 hours after delivery, the milk fat rate of the experimental group was higher than that of the control group, especially the milk fat rate of colostrum of the 5g AKG group and the 10g AKG group was significantly higher than that of the control group. At 4, 7 and 14 days after delivery, the milk fat rate of each group decreased and stabilized compared with 2 hours after delivery, and there was no significant difference.

[0104] Milk protein rate ( Figure 6 B) Within 2 hours after delivery, the milk protein rate of the experimental group was higher than that of the control group, especially the milk protein rate of the colostrum of the group fed 5g AKG was significantly higher than that of the control group. At 4, 7 and 14 days after delivery, the milk protein rate of each group decreased and stabilized compared with 2 hours after delivery, and there was no significant difference.

[0105] Lactose rate ( Figure 6 C) Within 2 hours after delivery, the lactose rate of the experimental group was lower than that of the control group, especially the lactose rate of colostrum of the 5g AKG group and the 10g AKG group was significantly lower than that of the control group 2 hours after delivery. On days 4, 7, and 14 after delivery, the lactose rate of each group increased and stabilized without significant difference.

[0106] Urea nitrogen ( Figure 6 D) Within 2 hours after delivery, the lactose rate of the experimental group was lower than that of the control group, especially the urea nitrogen of the colostrum of the group fed 5g AKG was significantly lower than that of the control group. At 4 days after delivery, the urea nitrogen level of each group increased and stabilized.

[0107] Somatic cell count ( Figure 6 E). Within 2 hours after delivery, the number of somatic cells in colostrum of the experimental group was significantly lower than that of the control group. At 4 days after delivery, the number of somatic cells in colostrum of the experimental group was lower than that of the control group, especially the number of somatic cells in colostrum of the group fed 10g AKG was significantly lower than that of the control group.

[0108] Daily milk production ( Figure 6F). On the 15th and 30th days after delivery, the daily milk production of the experimental group was higher than that of the control group, especially the daily milk production of the group fed with 10g AKG was significantly higher than that of the control group.

[0109] The above results show that feeding α-ketoglutarate in the early peripartum period not only significantly increased the daily milk production of dairy cows, but also improved the quality of milk components. Especially in the colostrum stage, the addition of α-ketoglutarate has an important regulatory effect on key indicators such as milk fat, milk protein, lactose, urea nitrogen and somatic cell count. This effect provides a reliable guarantee for improving the lactation performance of dairy cows and improving the health of calves, and has significant production and economic benefits.

[0110] The above description is only a general description of the present invention and a description of a specific embodiment, and does not limit the present invention in other forms. Any person skilled in the art can modify or modify the present invention based on the technical content disclosed in the present invention to an equivalent embodiment. Without departing from the concept and spirit of the present invention, any modification or modification of the present invention belongs to the protection scope of the technical solution of the present invention.

Claims

1. Use of α-ketoglutarate in improving postpartum reproductive performance and / or lactation performance of mammals.

2. The use according to claim 1, characterized in that The α-ketoglutaric acid has at least one of the following uses: (1) Improve the anti-inflammatory properties of mammals; (2) Improve the antioxidant capacity of mammals; (3) Improve protein metabolism and energy metabolism in periparturient dairy cows; (4) reduce the incidence of postpartum diseases in mammals; (5) Improve the postpartum pregnancy rate of mammals and shorten the number of days after mating; (6) Improve the ability of mammals to initiate lactation after childbirth; (7) increasing the milk fat and / or milk protein content of postpartum colostrum of mammals; (8) reducing the lactose and / or urea nitrogen content of postpartum colostrum of mammals; (9) Increase the daily milk production of mammals in the early lactation period; (10) Reducing the number of somatic cells in mammalian milk; (11) Increasing the birth weight of newborn mammals; (12) Reduce the incidence of diseases in newborn mammals; (13) Improve the survival rate of newborn mammals.

3. The use according to claim 2, characterized in that: The diseases include one or more of retained placenta, metritis, mastitis, postpartum paralysis, ketosis and diarrhea.

4. The use according to any one of claims 1 to 3, characterized in that The mammals include livestock, Preferably, the mammal comprises a pig, a cow, a sheep, a horse, a donkey or a rabbit, preferably a cow.

5. A method for improving the postpartum reproductive performance and / or lactation performance of a mammal, comprising the step of administering α-ketoglutarate to a peripartum mammal.

6. The method according to claim 5, characterized in that The α-ketoglutarate is administered by feeding, Preferably, the dosage of α-ketoglutaric acid is 0.5-20 g / day, preferably 1-10 g / day, more preferably 5-10 g / day.

7. The method according to claim 5 or 6, characterized in that: The method comprises feeding alpha-ketoglutarate to a mammal for 10-16 days prior to parturition and stopping feeding after the mammal has parturition.

8. The method according to any one of claims 5 to 7, characterized in that: The mammals include livestock, Preferably, the mammal comprises a pig, a cow, a sheep, a horse, a donkey or a rabbit, preferably a cow.

9. The method according to any one of claims 5 to 8, characterized in that: The improving of the postpartum reproductive performance and / or lactation performance of mammals comprises at least one of the following: (1) Improve the anti-inflammatory properties of mammals; (2) Improve the antioxidant capacity of mammals; (3) Improve protein metabolism and energy metabolism in periparturient dairy cows; (4) reduce the incidence of postpartum diseases in mammals; (5) Improve the postpartum pregnancy rate of mammals and shorten the number of days after mating; (6) Improve the ability of mammals to initiate lactation after childbirth; (7) increasing the milk fat and / or milk protein content of postpartum colostrum of mammals; (8) reducing the lactose and / or urea nitrogen content of postpartum colostrum of mammals; (9) Increase the daily milk production of mammals in the early lactation period; (10) Reducing the number of somatic cells in mammalian milk; (11) Increasing the birth weight of newborn mammals; (12) Reduce the incidence of diseases in newborn mammals; (13) Improve the survival rate of newborn mammals.

10. The method according to claim 9, characterized in that The diseases include one or more of retained placenta, metritis, mastitis, postpartum paralysis, ketosis and diarrhea.

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