Amino acid repairing composition and application thereof
By using amino acid repair compositions, the strength reduction and combing difficulties caused by hair damage are solved, and the effects of enhancing the tensile strength of hair, reducing combing work and increasing the denaturation temperature are achieved.
Patent Information
- Application Number
- CN202411894568.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-20
- Publication Date
- 2025-04-11
AI Technical Summary
After sun exposure, unreasonable combing and perming, hair is easily damaged, which is manifested as a decrease in tensile strength, weakening of elasticity and toughness, loss of color and gloss, and roughening of the surface texture.
An amino acid repair composition is provided, comprising an amino acid complex and a rock acacia leaf extract, with a mass ratio of (1 to 2): (1 to 3). The amino acid complex consists of histidine and phenylalanine, and the mass ratio is (60-80): (20-40). The composition is prepared by heating dissolution, concentration and drying.
The composition can enhance the tensile strength of damaged hair strands, reduce combing work, increase denaturation temperature, and have a synergistic antioxidant effect, significantly improve the strength and combability of hair.
Smart Images
Figure CN119792112A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of cosmetics, and particularly relates to an amino acid repair composition and application thereof. Background Art
[0002] Hair is a polymer fiber formed by completely keratinized keratinocytes; specifically, it is composed of keratin, pigments, water, lipids, non-keratin proteins, polysaccharides and trace elements, among which keratin has the largest content. Keratinocytes are filled with keratin composed of various amino acids, the amino acid with the highest content is cystine, which contains disulfide bonds. These amino acids are cross-linked through a spiral and spring-like structure to form the toughness and strength of keratin, giving hair resistance to chemicals, mechanical forces and the environment.
[0003] Hair grows and falls out over a period of several years. External factors such as the environment, pollution, and hair treatments can affect the nature of hair. Exposure to direct sunlight and ultraviolet rays can damage hair, as can improper combing. The most serious damage generally occurs when hair is dyed, permed, or styled. Hair damage is mainly manifested in decreased tensile strength, decreased elasticity and toughness, loss of color and luster, and roughened surface texture.
[0004] In view of these problems, developing a hair repair composition that can effectively improve the strength of hair and reduce the work of combing hair is of great significance to the development of hair products. Summary of the invention
[0005] The purpose of this section is to summarize some aspects of embodiments of the present invention and to briefly introduce some preferred embodiments.
[0006] As one aspect of the present invention, the present invention provides an amino acid repair composition, wherein: the amino acid repair composition is composed of an amino acid complex and a TOFI ELDIA JAPONICA leaf extract, and the mass ratio of the amino acid complex to the TOFI ELDIA JAPONICA leaf extract is (1-2): (1-3).
[0007] As a preferred embodiment of the amino acid repair composition of the present invention: the amino acid complex is composed of histidine and phenylalanine, and the mass ratio of histidine to phenylalanine is (60-80): (20-40).
[0008] As a preferred embodiment of the amino acid repair composition of the present invention, the mass ratio of histidine to phenylalanine is (65-70): (30-35).
[0009] As a preferred embodiment of the amino acid repair composition of the present invention: the preparation method of the amino acid complex comprises adding histidine and phenylalanine into water, heating to dissolve the amino acids, mixing evenly and concentrating to obtain the amino acid complex.
[0010] As a preferred embodiment of the amino acid repair composition of the present invention: histidine and phenylalanine are added into water with a solid-liquid ratio of 1:10-30.
[0011] As a preferred embodiment of the amino acid repair composition of the present invention: the preparation method of the Acorus calamus leaf extract comprises extracting the Acorus calamus leaves with 70-90wt% ethanol solution, taking the supernatant, and drying to obtain the Acorus calamus leaf extract.
[0012] As a preferred embodiment of the amino acid repair composition of the present invention: crush the Acorus calamus leaves and add them into a 70-90wt% ethanol solution, extract at 40-70°C for 1-6h, centrifuge to obtain the supernatant, concentrate and dry to obtain the Acorus calamus leaf extract.
[0013] As a preferred embodiment of the amino acid repair composition of the present invention: the Acorus calamus leaves are crushed and added into a 70-90 wt % ethanol solution, and the solid-liquid ratio is 1:10-60.
[0014] Beneficial effects of the invention: The invention studies a preparation method of an amino acid repair composition and the repair effect of the composition on damaged hair. Experimental results show that the amino acid repair composition can synergistically enhance the tensile strength of damaged hair bundles, reduce the combing work of damaged hair, increase the denaturation temperature of damaged hair bundles, and has a synergistic antioxidant effect. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] In order to more clearly illustrate the technical solution of the embodiment of the present invention, the following briefly introduces the drawings required for describing the embodiment, wherein:
[0016] Figure 1 The results of hair tensile strength test after using Acorus calamus leaf extracts using different extraction methods.
[0017] Figure 2 The figures are the combing power change results after using various embodiments and comparative examples.
[0018] Figure 3 The following are the test results of hair degeneration temperature after using various embodiments and comparative examples. DETAILED DESCRIPTION
[0019] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with specific embodiments.
[0020] Embodiment 1:
[0021] In the following examples and comparative examples, histidine and phenylalanine were purchased from the market.
[0022] (1) Preparation method of amino acid complex: Preparation method of compound raw material of histidine and phenylalanine: put histidine and phenylalanine in a mass ratio of 65:35 into a water bath, add water 15 times the mass of amino acids, heat at 80°C until the amino acids are completely dissolved, concentrate under reduced pressure until there is no liquid, spin out and set aside.
[0023] (2) Preparation method of Acorus calamus leaf extract:
[0024] Method 1: The Acorus calamus leaves dried to no weight change were crushed, passed through an 80-mesh sieve, and placed in a dryer for later use. Weigh 10 g of dried Acorus calamus leaf powder sample, add 70 wt% ethanol at a solid-liquid ratio of 1:30 (g / ml) at 50°C for 2 h, centrifuge at 5000 rpm for 10 min, take the supernatant, repeat the extraction 4 times, combine the extracts, concentrate under reduced pressure until there is no liquid, and freeze-dry the concentrated product to obtain the Acorus calamus leaf extract.
[0025] Method 2: The Acorus calamus leaves dried to no weight change were crushed, passed through an 80-mesh sieve, and placed in a dryer for later use. Weigh 10 g of dried Acorus calamus leaf powder sample, add 90 wt% ethanol at a solid-liquid ratio of 1:30 (g / ml) at 50°C for 2 h, centrifuge at 5000 rpm for 10 min, take the supernatant, repeat the extraction 4 times, combine the extracts, concentrate under reduced pressure until there is no liquid, and freeze-dry the concentrated product to obtain the Acorus calamus leaf extract.
[0026] Method 3: The Acorus calamus leaves dried to no weight change were crushed, passed through an 80-mesh sieve, and placed in a dryer for later use. Weigh 10 g of dried Acorus calamus leaf powder sample, add 50 wt% ethanol at a solid-liquid ratio of 1:30 (g / ml) at 50°C for 2 h, centrifuge at 5000 rpm for 10 min, take the supernatant, repeat the extraction 4 times, combine the extracts, concentrate under reduced pressure until there is no liquid, and freeze-dry the concentrated product to obtain Acorus calamus leaf extract.
[0027] Method 4: The Acorus calamus leaves dried to no weight change were crushed, passed through an 80-mesh sieve, and placed in a dryer for later use. Weigh 10 g of dried Acorus calamus leaf powder sample, add 30 wt% ethanol at a solid-liquid ratio of 1:30 (g / ml) at 50°C for 2 h, centrifuge at 5000 rpm for 10 min, take the supernatant, repeat the extraction 4 times, combine the extracts, concentrate under reduced pressure until there is no liquid, and freeze-dry the concentrated product to obtain Acorus calamus leaf extract.
[0028] The amino acid repair compositions of Example 1 and Comparative Examples 1-4 were prepared according to Table 1.
[0029] Table 1
[0030]
[0031] Testing the hair strengthening effects under different extraction conditions:
[0032] Hair strength test method:
[0033] Severely damaged hair strands were selected and stored under constant temperature and humidity conditions of (25±2)°C and (50±5)%.
[0034] Experimental group: Apply a certain amount of sample to the hair for 3 minutes and rinse with warm water. Each time the hair is rinsed with warm water, use your hands to brush the hair ten times with similar force. Then dry it under constant temperature and humidity conditions. This is one operation. Each group of hair is treated with the product five times.
[0035] The blank control group consisted of hair bundles treated with clean water, and other experimental operations were the same as those of the experimental group.
[0036] After 12 hours of storage under constant temperature and humidity conditions, the tensile test was performed. The diameter of the hair was measured using an SN-1200W high-definition camera. The specific method was to take the average of the diameters at three locations in the middle section. Thirty hair strands with a diameter difference of less than 10 μm were selected from the hair bundle for a single fiber strength test using a fiber strength tester. The tensile strength of the hair in the control group and each sample group was calculated and compared using the following formula:
[0037] σ=Fb / So
[0038] Where σ is the tensile strength, Fb is the maximum force the specimen withstands when it is broken, and So is the original cross-sectional area of the specimen.
[0039] The hair tensile strength test is shown in Table 2.
[0040] Table 2
[0041] Tensile strength / MPa P-value Example 1 225.43±9.89 Comparative Example 1 200.93±6.49 ＊ Comparative Example 2 186.90±6.39 ＊＊ Comparative Example 3 175.58±8.15 ＊＊
[0042] Note: The proportions in the table are compared with those in Example 1. ＊ indicates p<0.05, ＊＊ Indicates p < 0.01, ＊＊＊ Indicates p<0.001.
[0043] As shown in Table 2 and Figure 1 As shown, compared with the comparative example, the amino acid repairing composition of Acorus calamus leaf extract prepared by method 1 has a better effect on strengthening hair and has a significant difference.
[0044] The amino acid repair compositions of Examples 2-3 and Comparative Examples 4-5 were prepared according to Table 3, and the hair tensile strength, combing work and denaturation temperature were tested.
[0045] Table 3 Component types and proportions of the composition
[0046]
[0047]
[0048] The hair tensile strength was tested in the same manner as above. The experimental results are shown in Table 4.
[0049] Table 4
[0050] Tensile strength / MPa P-value Blank control group 170.03±13.15 ＊＊ Example 1 225.43±9.89 Example 2 224.60±7.90 Example 3 227.13±6.23 Comparative Example 4 184.63±7.06 ＊＊ Comparative Example 5 180.83±7.45 ＊＊
[0051] Note: The proportions in the table are compared with those in Example 1. ＊ indicates p<0.05, ＊＊ Indicates p < 0.01, ＊＊＊ Indicates p<0.001.
[0052] As shown in Table 4, the tensile strength of Examples 1-3 is higher than that of the control group, and there is a significant difference, indicating that the amino acid repair composition is beneficial to improving the strength of hair. The tensile strength of Examples 1-3 is higher than that of Comparative Examples 4-5, indicating that the amino acid complex and the Acorus calamus leaf extract synergistically improve the strength of hair.
[0053] Hair combing performance test method:
[0054] Severely damaged hair strands were selected and stored under constant temperature and humidity conditions of (25±2)°C and (50±5)%.
[0055] Use a comb to comb the hair repeatedly 10 times to separate the hair roots and remove the knots.
[0056] The combability of each hair bundle was measured by a hair combing instrument. Each hair bundle was tested three times, and the average value was taken as the initial value of the hair bundle. The hair bundles with large deviations in the initial value were eliminated, and at least 5 valid initial values (A 用样前 ).
[0057] Experimental group: Wet the hair with clean water, apply a certain amount of the sample to be tested for 1 minute, and rinse it with warm water. Each time you rinse with warm water, use your hands to brush your hair ten times with similar force. This is one operation. Each group of hair is treated with the product five times.
[0058] Use a comb to gently comb the wet hair bundle, and hang the hair bundle in a constant temperature and humidity chamber with a temperature of (20-25)°C and a relative humidity of 40%-60% for 12 hours.
[0059] The blank control group consisted of hair bundles treated with clean water, and other experimental operations were the same as those of the experimental group.
[0060] The combability measurement value (A 用样后 ), the number of tests is kept consistent with the number of initial value tests before using the sample.
[0061] The change rate K value of the combing work before and after the sample was less than 0, and the combing work showed a statistically significant difference, indicating that the sample improved the combing property of the hair.
[0062] K value = (A 用样后 -A 用样前 ) / A 用样前
[0063] The hair combing force test is shown in Table 5.
[0064] Table 5
[0065] K Value P-value Control group -0.16 Example 1 -0.64 ＊＊＊ Example 2 -0.62 ＊＊＊ Example 3 -0.63 ＊＊＊ Comparative Example 4 -0.20 Comparative Example 5 -0.21
[0066] Note: The proportion in Table 5 is A 用样后 With A 用样前 compared to, ＊ indicates p<0.05, ＊＊ Indicates p < 0.01, ＊＊＊ Indicates p<0.001.
[0067] As shown in Table 5 and Figure 2 As shown, the K values of Examples 1-3 are less than 0 and lower than that of the control group, indicating that the amino acid repair composition can significantly reduce the combing work of the hair bundle and improve the combability of damaged hair.
[0068] The combing work of the hair bundles in Examples 1-3 before and after the samples were significantly different, indicating the necessity of compounding the amino acid complex and the Acorus calamus leaf extract.
[0069] Hair denaturation temperature test method:
[0070] Severely damaged hair strands were selected and stored under constant temperature and humidity conditions of (25±2)°C and (50±5)%.
[0071] Experimental group: The hair was smeared with a fixed amount of product for 3 minutes and then rinsed with warm water. Each time the hair was rinsed with warm water, it was brushed ten times with similar force by hand. After that, it was dried under constant temperature and humidity conditions. This was considered one operation. Each group of hair was treated with the product five times.
[0072] The control group consisted of hair bundles treated with clean water.
[0073] The denaturation temperature of hair strands was measured using a DSC instrument.
[0074] The hair was cut into 5-10 mg pieces and quickly transferred to the DSC instrument. The lid of the sample pan was perforated to allow the evaporated water to escape. All experiments were performed under a constant flow of N2. The samples were heated at 10 °C min -1 The hair strands were heated from 30°C to 300°C at a rate of 1 200 ℃ and the heat flow was recorded. The hair strands were kept in a humidity controlled cabinet (22°C, 60% RH) for 48 h before all characterizations.
[0075] The hair denaturation temperature experiment is shown in Table 6.
[0076] Table 6
[0077] Denaturation temperature / ℃ P-value Control group 128.67±2.1 <![CDATA[ ＊＊＊ ]]> Example 1 148.00±1.6 Example 2 146.67±3.4 Example 3 147.67±1.2 Comparative Example 4 131.00±2.2 <![CDATA[ ＊＊＊ ]]> Comparative Example 5 130.33±3.1 <![CDATA[ ＊＊ ]]>
[0078] Note: The proportions in Table 6 are compared with those in Example 1. ＊ indicates p<0.05, ＊＊ Indicates p < 0.01, ＊＊＊ Indicates p<0.001.
[0079] As shown in Table 6 and Figure 3 As shown, the denaturation temperatures of Examples 1-3 are significantly higher than that of the control group, indicating that the amino acid repair composition can increase the denaturation temperature of hair and strengthen the internal structure of hair.
[0080] The denaturation temperatures of Examples 1-3 are significantly higher than those of Comparative Examples 4-5, and the difference is significant, indicating that the amino acid complex and the Acorus calamus leaf extract have a synergistic effect.
[0081] Verification of the synergistic antioxidant effect of the composition:
[0082] DPPH free radical scavenging test:
[0083] DPPH is a stable free radical that is purple in organic solvents and has a large absorption at a wavelength of 517nm. When antioxidants are added, some free radicals will be removed, causing the absorption at this wavelength to weaken, which can be used to evaluate the antioxidant properties of the sample to be tested.
[0084] (1) Take 50 mg of the sample to be tested, dissolve it in anhydrous ethanol and make up to 50 mL; prepare a solution of each sample to be tested with a mass concentration of 1 mg / mL, and then use this mass concentration to prepare the required mass concentration.
[0085] (2) Prepare 0.25 μmol / L DPPH solution with anhydrous ethanol and place it in a brown volumetric flask.
[0086] (3) Take 2 mL of the sample solution to be tested and add 2 mL of DPPH solution. Heat in a 25°C water bath for 60 min. Measure the sample absorbance (A) at a wavelength of 517 nm. i );
[0087] (4) Take 2 mL of distilled water instead of the sample to measure the blank absorbance (A0);
[0088] (5) Add 2 mL of distilled water to 2 mL of sample and measure the sample background absorbance (A j ),
[0089] (6) Three parallel experiments were performed for each sample concentration and the average value was taken. The clearance rate was calculated according to the following formula.
[0090]
[0091] Table 7
[0092]
[0093]
[0094]
[0095]
[0096] The compound joint action index analysis is calculated using the median effect principle. The formula for the joint action index is:
[0097]
[0098] (D)1——the concentration of compound 1 required to produce effect X when used in combination with other compounds;
[0099] (D)2——the concentration of compound 2 required to produce effect X when used in combination with other compounds;
[0100] (D x )1——the concentration of compound 1 required to produce effect X when used alone;
[0101] (D x )2——the concentration of compound 2 required to produce effect X when used alone;
[0102] CI value interval of combined effect and its interaction evaluation:
[0103]
[0104]
[0105] The CI index of the formulas of Group I and Group II in Table 7 is calculated according to the calculation formula, and the result is: CI I =0.478, CI II =0.463, CI III=0.586, indicating that the amino acid complex and Acorus calamus leaf extract used in a certain proportion have a synergistic effect of scavenging DPPH free radicals.
[0106] The amino acid complex and Acorus calamus leaf extract had a synergistic antioxidant effect in the mass ratio range of 10-20:10-30. It is speculated that this may be because the Acorus calamus leaf extract contains a variety of active ingredients, such as phenylpropanoid compounds and isocoumarin compounds, which may form a complex or non-covalent binding after compounding with amino acids, thereby synergistically improving the antioxidant activity.
[0107] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.
Claims
1. An amino acid repair composition, characterized in that: The amino acid repair composition consists of an amino acid complex and a rock calamus leaf extract, wherein the mass ratio of the amino acid complex to the rock calamus leaf extract is (1-2):(1-3).
2. The amino acid repair composition according to claim 1, characterized in that: The amino acid complex consists of histidine and phenylalanine, and the mass ratio of histidine to phenylalanine is (60-80):(20-40).
3. The amino acid repair composition according to claim 2, characterized in that: The mass ratio of histidine to phenylalanine is (65-70):(30-35).
4. The amino acid repair composition according to claim 2 or 3, characterized in that: The preparation method of the amino acid complex comprises adding histidine and phenylalanine into water, heating to dissolve the amino acids, mixing evenly and concentrating to obtain the amino acid complex.
5. The amino acid repair composition according to claim 4, characterized in that: The histidine and phenylalanine are added into water, and the solid-liquid ratio is 1:10-30.
6. The amino acid repair composition according to any one of claims 1 to 3, characterized in that: The preparation method of the Acorus calamus leaf extract comprises the following steps: extracting the Acorus calamus leaves with 70-90 wt % ethanol solution, taking the supernatant, and drying to obtain the Acorus calamus leaf extract.
7. The amino acid repair composition according to claim 6, characterized in that: The Acorus calamus leaves are crushed and added into a 70-90 wt% ethanol solution, extracted at 40-70° C. for 1-6 hours, centrifuged to obtain the supernatant, concentrated and dried to obtain the Acorus calamus leaf extract.
8. The amino acid repair composition according to claim 7, characterized in that: The rock calamus leaves are crushed and added into a 70-90wt% ethanol solution, and the solid-liquid ratio is 1:10-60.
9. Use of the amino acid repair composition according to claim 1 in the preparation of cosmetics.
10. The use according to claim 9, characterized in that: The cosmetics include shampoo products, scalp care products, and hair care products.