Composition with anti-inflammatory and anti-aging effects and its application

Abstract

The present invention relates to a composition with anti-inflammatory and anti-aging effects and its application. The composition comprises a cornus officinalis extract, a quinquefolia extract, and a guava extract. The composition can effectively scavenge free radicals, inhibit collagenase activity, inhibit the production of fluorescent AGEs, and prevent skin sagging and dullness caused by skin aging and glycation reactions. At the same time, the composition can alleviate skin inflammatory reactions and improve skin microcirculation by inhibiting the secretion of NO. The synergistic effect of the various ingredients significantly enhances the antioxidant, anti-inflammatory, anti-aging, and anti-glycation effects, providing a comprehensive solution to skin problems. This process does not use any organic solvents, conforms to the concept of green environmental protection, ensures the safety and reliability of the extract, and provides a gentle and efficient solution for anti-aging.

Description

Technical Field

[0001] The invention belongs to the technical field of plant extraction, and relates to a composition with anti-inflammatory and anti-aging effects and application thereof. Background Art

[0002] Skin aging is a complex and gradual physiological process, influenced by the interaction of multiple internal and external factors. External factors such as human metabolism and ultraviolet radiation lead to the massive production of free radicals. These free radicals are highly oxidative and can attack various biomolecules in skin cells, affecting skin cell renewal and repair, and ultimately accelerating the aging process. When the body's balance is disturbed, collagenase activity becomes unbalanced, leading to excessive breakdown of collagen, which reduces the collagen content in the skin, loosens the skin's fiber structure, and loses elasticity. At the same time, the accumulation of AGEs (advanced glycation end products) in the skin not only makes the skin stiff and loses elasticity, but also exacerbates dullness and yellowing. Excessive AGEs induce oxidative stress, further increasing the production of free radicals, stimulating the release of inflammatory factors, and activating the expression of collagenase, forming a vicious cycle that accelerates skin aging and damage.

[0003] Based on this, the present invention aims to provide a composition with anti-inflammatory and anti-aging effects and its application, aiming to improve the aging state of the skin through multi-faceted regulation, inhibit the generation of free radicals and the accumulation of AGEs, thereby effectively preventing and alleviating skin aging-related problems. Summary of the Invention

[0004] To address the shortcomings of the existing technology, the present invention aims to provide a composition with anti-inflammatory and anti-aging effects and its use. By scientifically selecting natural plant extracts with significant antioxidant, anti-aging, and anti-inflammatory effects and combining their synergistic effects, the present invention can improve skin health in a multi-dimensional and comprehensive manner, preventing and delaying the skin aging process.

[0005] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a composition with anti-inflammatory and anti-aging effects, comprising a Cornus officinalis extract, a Prunus mume extract, and a Psidium guajava extract.

[0007] Cornus officinalis (Cornus officinalis Sieb.et Zucc.) is a plant of the genus Cornus in the family Cornaceae. Studies have found that the pulp of Cornus officinalis is rich in iridoid ether glycosides, triterpenes, and polysaccharides. These ingredients have beneficial effects on the human body, such as lowering blood sugar, improving immunity, and having anti-tumor, anti-inflammatory, antibacterial and antioxidant effects.

[0008] Five-fingered ginseng (Ficus hirta Vahl.), a member of the Moraceae family, is a traditional medicinal and edible plant in South China. Its main chemical components, including coumarins, polysaccharides, steroids, phenols, and flavonoids, possess various pharmacological effects, including anti-inflammatory, analgesic, immune-enhancing, hepatoprotective, antibacterial, and antioxidant properties.

[0009] Guava (Psidium guajava L.) is a tree plant of the genus Psidium in the Myrtaceae family. It is rich in various nutrients, including polyphenols, flavonoids, polysaccharides, vitamins, trace elements, etc. These substances play an important role in physical health and beauty. In addition, guava can also effectively promote the balance of blood sugar in the human body and have a positive impact on the prevention and management of chronic diseases.

[0010] Cornus officinalis, ginseng, and guava are all raw materials that can be used as both medicine and food. These natural ingredients can comprehensively improve skin health through scientific proportions and synergistic effects. They not only improve skin condition through direct antioxidant and anti-inflammatory effects, but also fundamentally prevent and delay the skin aging process by regulating the body's internal physiological functions.

[0011] Preferably, the composition comprises, by mass, 10-50 parts of Cornus officinalis extract, 20-60 parts of Prunus mume extract and 10-50 parts of Psidium guajava extract.

[0012] The mass fraction of the Cornus officinalis extract can be selected from 10 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, etc., the mass fraction of the Prunus armeniaca extract can be selected from 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, 55 parts, 60 parts, etc., and the mass fraction of the guava extract can be selected from 10 parts, 15 parts, 20 parts, 25 parts, 30 parts, 35 parts, 40 parts, 45 parts, 50 parts, etc. Other specific point values within the above numerical range can be selected, and they will not be repeated here.

[0013] Preferably, the Cornus officinalis extract is prepared by a method comprising the following steps:

[0014] (1) Mixing Cornus officinalis with water and performing ultrahigh pressure microfluidization pretreatment;

[0015] (2) mixing the pretreated Cornus officinalis with an enzyme for enzymolysis to obtain an enzymolysis solution;

[0016] (3) The enzymatic hydrolysate is filtered, concentrated, and dried to obtain the product.

[0017] Enzymatic hydrolysis can help release the active ingredients in the cells. By controlling the enzymatic hydrolysis conditions, the enzymatic hydrolysis efficiency can be maximized to avoid destroying the active ingredients.

[0018] Preferably, the Prunus mume extract is prepared by a method comprising the following steps:

[0019] (1) mixing the five-fingered ginseng with water, and extracting under ultrasound to obtain an extract;

[0020] (2) The extract is filtered, concentrated, and dried to obtain the product.

[0021] Ultrasonic waves generate strong vibration and cavitation effects, which help to destroy cell structures and accelerate the release and extraction of active ingredients. By controlling the ultrasonic conditions, the extraction efficiency and product purity can be maximized.

[0022] Preferably, the guava extract is prepared by a method comprising the following steps:

[0023] (1) mixing guava with water and performing ultrahigh pressure microfluidization pretreatment;

[0024] (2) subjecting the pretreated guava to reflux extraction to obtain an extract;

[0025] (3) The extract is centrifuged to obtain the supernatant, which is filtered, concentrated, and dried to obtain the product.

[0026] Preferably, the material-liquid ratio of Cornus officinalis to water is 1g:(10-30)mL, wherein 10, 15, 20, 25, 30, etc. in (10-30) and other specific values within the above numerical range can be selected, which will not be repeated here.

[0027] Preferably, the ultra-high pressure microfluidic pretreatment is performed at a pressure of 100-300 MPa, a temperature of 60-80° C., and a number of times of 2-6 times.

[0028] The pressure can be selected from 100MPa, 120MPa, 140MPa, 160MPa, 180MPa, 200MPa, 220MPa, 240MPa, 260MPa, 280MPa, 300MPa, etc., the temperature is 60℃, 62℃, 64℃, 66℃, 68℃, 70℃, 72℃, 74℃, 76℃, 78℃, 80℃, etc., the number of times can be selected from 2 times, 3 times, 4 times, 5 times, 6 times, etc. Other specific point values within the above numerical range can be selected, and they will not be repeated here.

[0029] Preferably, the enzyme is a combination of cellulase and pectinase, and the amount of enzyme added is 2-5% of the mass of Cornus officinalis, for example, 2%, 2.2%, 2.5%, 2.8%, 3%, 3.2%, 3.5%, 3.8%, 4%, 4.2%, 4.5%, 4.8%, 5%, etc. Other specific point values within the above numerical range can be selected, and they will not be repeated here.

[0030] Preferably, the enzyme activity ratio of cellulase and pectinase is 1:(1-2), for example 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2, etc. Other specific point values within the above numerical range can be selected, and they will not be repeated here.

[0031] Preferably, the enzymatic hydrolysis temperature is 35-55° C., the pH is 3-5.5, and the time is 20-30 min.

[0032] The temperature can be selected from 35°C, 37°C, 40°C, 42°C, 44°C, 46°C, 48°C, 50°C, 52°C, 55°C, etc., the pH can be selected from 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, 4.8, 5, 5.2, 5.5, etc., and the time can be selected from 20min, 21min, 22min, 23min, 24min, 25min, 26min, 27min, 28min, 29min, 30min, etc. Other specific point values within the above numerical range can be selected, so they will not be listed here one by one.

[0033] Preferably, the enzymatic hydrolysis further comprises keeping the mixture at 78-85° C. for 30-50 minutes.

[0034] The temperature can be selected from 78°C, 79°C, 80°C, 81°C, 82°C, 83°C, 84°C, 85°C, etc., and the time can be selected from 30min, 32min, 34min, 36min, 38min, 40min, 42min, 44min, 46min, 48min, 50min, etc. Other specific point values within the above numerical range can be selected, so they will not be listed here one by one.

[0035] Preferably, the filtration comprises filtering through a screen, a ceramic membrane and an ultrafiltration membrane in sequence.

[0036] Preferably, the aperture of the sieve is 400-600 mesh, for example, 400 mesh, 420 mesh, 440 mesh, 460 mesh, 480 mesh, 500 mesh, 520 mesh, 540 mesh, 560 mesh, 580 mesh, 600 mesh, etc. Other specific point values within the above numerical range can be selected, and they will not be repeated here.

[0037] Preferably, the pore size of the ceramic membrane is 100-200nm, for example, 100nm, 110nm, 120nm, 130nm, 140nm, 150nm, 160nm, 170nm, 180nm, 190nm, 200nm, etc. Other specific point values within the above numerical range can be selected, and they will not be described here one by one.

[0038] Preferably, the pore size of the ultrafiltration membrane is 1000-5000Da, for example, 1000Da, 1500Da, 2000Da, 2500Da, 3000Da, 3500Da, 4000Da, 4500Da, 5000Da, etc. Other specific point values within the above numerical range can be selected, and they will not be repeated here.

[0039] Preferably, the vacuum degree of the concentration is 60-110 mbar and the temperature is 50-60°C.

[0040] The vacuum degree can be selected as 60mbar, 65mbar, 70mbar, 75mbar, 80mbar, 85mbar, 90mbar, 95mbar, 100mbar, 105mbar, 110mbar, etc., and the temperature can be selected as 50℃, 51℃, 52℃, 53℃, 54℃, 55℃, 56℃, 57℃, 58℃, 59℃, 60℃, etc. Other specific point values within the above numerical range can be selected, so they will not be listed here.

[0041] Preferably, the material-liquid ratio of five-fingered peach to water is 1g:(10-30)mL, where 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, etc. in (10-30), and other specific point values within the above numerical range can be selected, so they will not be repeated here.

[0042] Preferably, before extraction, the method further comprises treating the mixture of the five-fingered ginseng and water at 110-130° C. and 0.1-0.2 MPa for 15-30 minutes.

[0043] The temperature can be selected as 110℃, 112℃, 114℃, 116℃, 118℃, 120℃, 122℃, 124℃, 126℃, 128℃, 130℃, etc. The pressure can be selected as 0.1MPa, 0.11MPa, 0.12MPa, 0.13MPa, 0.14MPa, 0.15MPa0.16MPa, 0.17MPa, 0.18MPa, 0.19MPa, 0.2MPa, etc. The time can be selected as 15min, 16min, 17min, 18min, 19min, 20min, 21min, 22min, 23min, 24min, 25min, 26min, 27min, 28min, 29min, 30min, etc. Other specific point values within the above numerical range can be selected, so they will not be listed here one by one.

[0044] Preferably, the temperature of the ultrasonic extraction is 60-80°C, the frequency is 30-40kHz, and the time is 15-30min.

[0045] The temperature can be selected as 60℃, 62℃, 64℃, 66℃, 68℃, 70℃, 72℃, 74℃, 76℃, 78℃, 80℃, etc. The frequency can be selected as 30kHz, 31kHz, 32kHz, 33kHz, 34kHz, 35kHz, 36kHz, 37kHz, 38kHz, 39kHz, 40kHz, etc. The time can be selected as 15min, 16min, 17min, 18min, 19min, 20min, 21min, 22min, 23min, 24min, 25min, 26min, 27min, 28min, 29min, 30min, etc. Other specific point values within the above numerical range can be selected, so they will not be listed here.

[0046] Preferably, the number of ultrasounds is 1-3 times, for example, 1 time, 2 times, 3 times, etc.

[0047] Preferably, the vacuum degree of the concentration is 60-110 mbar and the temperature is 50-60°C.

[0048] The vacuum degree can be selected as 60mbar, 65mbar, 70mbar, 75mbar, 80mbar, 85mbar, 90mbar, 95mbar, 100mbar, 105mbar, 110mbar, etc., and the temperature can be selected as 50℃, 51℃, 52℃, 53℃, 54℃, 55℃, 56℃, 57℃, 58℃, 59℃, 60℃, etc. Other specific point values within the above numerical range can be selected, so they will not be listed here.

[0049] Preferably, the material-liquid ratio of guava to water is 1:(10-30) mL, wherein the specific point values in (10-30) can be selected from 10, 15, 20, 25, 30, etc. Other specific point values within the above numerical range can be selected, and they will not be repeated here.

[0050] Preferably, the ultra-high pressure microfluidic pretreatment is performed at a pressure of 100-300 MPa, a temperature of 60-80° C., and a number of times of 3-6 times.

[0051] The pressure can be selected from 100MPa, 120MPa, 140MPa, 160MPa, 180MPa, 200MPa, 220MPa, 240MPa, 260MPa, 280MPa, 300MPa, etc., the temperature can be selected from 60℃, 62℃, 64℃, 66℃, 68℃, 70℃, 72℃, 74℃, 76℃, 78℃, 80℃, etc., the number of times can be selected from 3 times, 4 times, 5 times, 6 times, etc. Other specific point values within the above numerical range can be selected, so they will not be listed here.

[0052] Preferably, the reflux extraction time is 20-40 min.

[0053] The time can be selected as 20 minutes, 25 minutes, 30 minutes, 35 minutes, 40 minutes, etc. Other specific point values within the above numerical range can be selected, and they will not be described here one by one.

[0054] Preferably, the centrifugal speed is 5000-7000 rpm and the time is 5-10 min.

[0055] The rotation speed can be selected as 5000rpm, 5500rpm, 6000rpm, 6500rpm, 7000rpm, etc., and the time can be selected as 5min, 6min, 7min, 8min, 9min, 10min, etc. Other specific point values within the above numerical range can be selected, and they will not be repeated here.

[0056] Preferably, the filtering comprises filtering through a 100-200 nm ceramic membrane.

[0057] The ceramic membrane can be selected at 100nm, 110nm, 120nm, 130nm, 140nm, 150nm, 160nm, 170nm, 180nm, 190nm, 200nm, etc. Other specific point values within the above numerical range can be selected, and they will not be described one by one here.

[0058] Preferably, the vacuum degree of the concentration is 60-110 mbar and the temperature is 50-60°C.

[0059] The vacuum degree can be selected as 60mbar, 65mbar, 70mbar, 75mbar, 80mbar, 85mbar, 90mbar, 95mbar, 100mbar, 105mbar, 110mbar, etc., and the temperature can be selected as 50℃, 51℃, 52℃, 53℃, 54℃, 55℃, 56℃, 57℃, 58℃, 59℃, 60℃, etc. Other specific point values within the above numerical range can be selected, so they will not be listed here.

[0060] Compared with the prior art, the present invention has the following beneficial effects:

[0061] (1) The present invention effectively extracts the active ingredients from Cornus officinalis, Prunus mume and Psidium guajava through ultrahigh pressure microfluidic extraction, complex enzyme hydrolysis, high pressure extraction, ultrasound-assisted extraction and other steps, and successfully obtains a composition with anti-inflammatory, anti-aging, anti-glycation and microcirculation improving effects.

[0062] (2) The composition obtained by the preparation method of the present invention can significantly scavenge DPPH free radicals and inhibit collagenase activity.

[0063] (3) The composition obtained by the preparation method of the present invention can significantly inhibit the formation of fluorescent AGEs and reduce the relative expression level of NO.

[0064] (4) The composition obtained by the preparation method of the present invention can significantly promote blood circulation and improve microcirculation in zebrafish.

[0065] (5) The composition obtained by the preparation method of the present invention has synergistic effects in anti-oxidation, anti-aging, anti-inflammatory, anti-glycation and microcirculation improvement.

[0066] (6) The present invention uses RO water as the extraction solvent, which not only effectively extracts bioactive components from plants but also further expands its application in the field of health. No organic solvents are used throughout the process, eliminating the need for complex recycling and processing steps, significantly reducing energy consumption and pollutant emissions, effectively alleviating environmental pressure, and better meeting the needs of industrial production. DETAILED DESCRIPTION

[0067] The technical solution of the present invention is further described below by way of specific embodiments. It should be understood by those skilled in the art that the embodiments are merely to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0068] The sources of the functional ingredients contained in the products involved in the following examples and comparative examples are as follows (only the functional ingredients are reflected, and the necessary auxiliary ingredients contained in other commercially available raw materials are not repeated):

[0069] Cellulase was purchased from Wuxi Xuemei Enzyme Preparation Technology Co., Ltd. under the trade name of Cellulase.

[0070] Pectinase was purchased from Tianjin Lihua Enzyme Preparation Technology Co., Ltd. under the trade name of Pectinase.

[0071] Preparation Example 1-1

[0072] This preparation example provides a method for preparing a Cornus officinalis extract, the preparation method comprising:

[0073] (1) 10 g of Cornus officinalis was crushed and mixed with water at a material-liquid ratio of 1 g:20 mL. Ultrahigh pressure microfluidization pretreatment was performed at a pressure of 200 MPa and a temperature of 70°C for 4 times.

[0074] (2) The cornus officinalis obtained in step (1) was mixed with 0.4 g of the complex enzyme and enzymatically hydrolyzed at 45° C. and pH 4 for 25 min. After enzymatic hydrolysis, the mixture was kept at 80° C. for 40 min.

[0075] (3) The enzymatic hydrolysis product was filtered through a 400-mesh sieve, a 5 μm filter bag, a 100 nm ceramic membrane, and a 1000 Da ultrafiltration membrane in sequence. The retentate was concentrated to 15 wt% at 55° C. and a vacuum of 80 mbar, sterilized, and freeze-dried to obtain the product.

[0076] Preparation Example 1-2

[0077] This preparation example provides a method for preparing a Cornus officinalis extract, the preparation method comprising:

[0078] (1) 10 g of Cornus officinalis was crushed and mixed with water at a material-liquid ratio of 1 g:10 mL. Ultrahigh pressure microfluidization pretreatment was performed at a pressure of 100 MPa and a temperature of 60°C for 2 times.

[0079] (2) The cornus officinalis obtained in step (1) was mixed with 0.2 g of the complex enzyme and enzymatically hydrolyzed at 35° C. and pH 3 for 20 min. After enzymatic hydrolysis, the mixture was kept at 80° C. for 30 min.

[0080] (3) The enzymatic hydrolysis product was filtered through a 400-mesh sieve, a 5 μm filter bag, a 200 nm ceramic membrane, and a 3000 Da ultrafiltration membrane in sequence. The retentate was concentrated to 20 wt% at 50° C. and a vacuum degree of 60 mbar, sterilized, and freeze-dried to obtain the product.

[0081] Preparation Examples 1-3

[0082] This preparation example provides a method for preparing a Cornus officinalis extract, the preparation method comprising:

[0083] (1) 10 g of Cornus officinalis was crushed and mixed with water at a material-liquid ratio of 1 g:20 mL. Ultrahigh pressure microfluidization pretreatment was performed at a pressure of 300 MPa and a temperature of 80°C for 6 times.

[0084] (2) The cornus officinalis obtained in step (1) was mixed with 0.5 g of the complex enzyme and enzymatically hydrolyzed at 55° C. and pH 5.5 for 30 min. After enzymatic hydrolysis, the mixture was kept at 80° C. for 50 min.

[0085] (3) The enzymatic hydrolysis product was filtered through a 400-mesh sieve, a 5 μm filter bag, a 150 nm ceramic membrane, and a 4000 Da ultrafiltration membrane in sequence. The retentate was concentrated to 10 wt % at 60° C. and a vacuum degree of 110 mbar, sterilized, and freeze-dried to obtain the product.

[0086] Preparation Examples 1-4

[0087] This preparation example provides a preparation method of Cornus officinalis extract, which differs from Preparation Example 1-1 only in that step (1) is "crushing 10 g of Cornus officinalis and mixing it with water, with the material-liquid ratio of Cornus officinalis to water being 1 g:20 mL", and other operations remain unchanged.

[0088] Preparation Examples 1-5

[0089] This preparation example provides a preparation method of Cornus officinalis extract, which differs from Preparation Example 1-1 only in that step (2) is "treating the Cornus officinalis obtained in step (1) at 45°C and pH 4 for 25 minutes, and then keeping the mixture at 80°C for 40 minutes", and other operations remain unchanged.

[0090] Preparation Examples 1-6

[0091] This preparation example provides a method for preparing a Cornus officinalis extract, which differs from Preparation Example 1-1 only in that step (3) is "filtering the enzymatic hydrolyzate obtained in step (2) through a 400-mesh sieve and a 5-μm filter bag, concentrating to 15 wt% at a temperature of 55° C. and a vacuum degree of 80 mbar, sterilizing, and freeze-drying to obtain the product." Other operations remain unchanged.

[0092] Preparation Examples 1-7

[0093] This preparation example provides a method for preparing a Cornus officinalis extract, which differs from Preparation Example 1-1 only in that step (3) is "filtering the enzymatic hydrolyzate obtained in step (2) through a 400-mesh sieve, a 5-μm filter bag, and a 100-nm ceramic membrane, taking the filtrate, concentrating it to 15 wt% at a temperature of 55° C. and a vacuum degree of 80 mbar, sterilizing it, and freeze-drying it to obtain the product." Other operations remain unchanged.

[0094] Preparation Examples 1-8

[0095] This preparation example provides a preparation method of Cornus officinalis extract, which differs from Preparation Example 1-1 only in that step (2) is "reflux extraction of the product obtained in step (1) for 25 minutes, twice", and other operations remain unchanged.

[0096] Preparation Example 2-1

[0097] This preparation example provides a preparation method of a Prunus mume extract, which comprises:

[0098] (1) Crush the five-fingered ginseng and mix it with water at a material-liquid ratio of 1 g:20 mL. Treat at 0.15 MPa and 120 °C for 20 min.

[0099] (2) The product obtained in step (1) was subjected to ultrasonic extraction at 70° C. for 20 min, with an ultrasonic frequency of 35 kHz and two times.

[0100] (3) The product obtained in step (2) was passed through a 400-mesh sieve and a 5 μm filter bag, concentrated to 10 wt % at 80 mbar and 55° C., sterilized, and dried.

[0101] Preparation Example 2-2

[0102] This preparation example provides a preparation method of a Prunus mume extract, which comprises:

[0103] (1) Crush the five-fingered ginseng and mix it with water at a material-liquid ratio of 1 g:10 mL. Treat at 0.1 MPa and 110 °C for 30 min.

[0104] (2) The product obtained in step (1) was subjected to ultrasonic extraction at 80° C. for 15 min, with an ultrasonic frequency of 40 kHz and three times.

[0105] (3) The product obtained in step (2) was passed through a 400-mesh sieve and a 5 μm filter bag, concentrated to 15 wt% at 60 mbar and 50° C., sterilized, and dried to obtain the product.

[0106] Preparation Example 2-3

[0107] This preparation example provides a preparation method of a Prunus mume extract, which comprises:

[0108] (1) Crush the five-fingered ginseng and mix it with water at a material-liquid ratio of 1 g:30 mL. Treat at 0.2 MPa and 130 °C for 15 min.

[0109] (2) The product obtained in step (1) was subjected to ultrasonic extraction at 60° C. for 30 min, with an ultrasonic frequency of 30 kHz and a number of times of 1.

[0110] (3) The product obtained in step (2) was passed through a 400-mesh sieve and a 5 μm filter bag, concentrated to 5 wt% at 110 mbar and 60° C., sterilized, and dried to obtain the product.

[0111] Preparation Example 2-4

[0112] This preparation example provides a preparation method of a five-fingered ginseng extract, which differs from Preparation Example 2-1 only in that step (1) is "crushing the five-fingered ginseng extract and mixing it with water, with the material-liquid ratio of the five-fingered ginseng extract to water being 1 g:20 mL", and other operations remain unchanged.

[0113] Preparation Example 2-5

[0114] This preparation example provides a preparation method of a Prunus mume extract, which differs from Preparation Example 2-1 only in that step (2) is "extracting the product obtained in step (1) at 70°C for 20 minutes, twice", and other operations remain unchanged.

[0115] Preparation Example 2-6

[0116] This preparation example provides a preparation method of a Prunus mume extract, which differs from Preparation Example 2-1 only in that step (2) is "reflux extraction of the product obtained in step (1) for 20 minutes, twice", and other operations remain unchanged.

[0117] Preparation Example 2-7

[0118] This preparation example provides a preparation method of a Prunus mume extract, which differs from Preparation Example 2-1 only in that step (2) is "subjecting the product obtained in step (1) to vacuum cavitation extraction for 1 h at a temperature of 40°C and a vacuum intensity of 90 mbar", and other operations remain unchanged.

[0119] Preparation Example 3-1

[0120] This preparation example provides a preparation method of guava extract, which comprises:

[0121] (1) Guava was crushed and mixed with water at a ratio of 1 g:15 mL. The guava was pretreated with ultrahigh pressure microfluidization at a temperature of 70° C. and a pressure of 200 MPa for 5 times.

[0122] (2) reflux extraction of the guava obtained in step (1) for 30 minutes;

[0123] (3) The product obtained by reflux extraction was centrifuged at 6000 rpm for 8 min, the supernatant was filtered through a 150 nm filter membrane, concentrated to 10 wt% at 55° C. and 80 mbar, sterilized, and dried to obtain the product.

[0124] Preparation Example 3-2

[0125] This preparation example provides a preparation method of guava extract, which comprises:

[0126] (1) Guava was crushed and mixed with water at a ratio of 1 g:10 mL. The guava was pretreated with ultrahigh pressure microfluidization at a temperature of 60° C. and a pressure of 100 MPa for 6 times.

[0127] (2) reflux extraction of the guava obtained in step (1) for 40 minutes;

[0128] (3) The product obtained by reflux extraction was centrifuged at 7000 rpm for 5 min, the supernatant was filtered through a 100 nm filter membrane, concentrated to 15 wt% at 50° C. and 60 mbar, sterilized, and dried to obtain the product.

[0129] Preparation Example 3-3

[0130] This preparation example provides a preparation method of guava extract, which comprises:

[0131] (1) Guava was crushed and mixed with water at a ratio of 1 g:20 mL. The guava was pretreated with ultrahigh pressure microfluidization at a temperature of 80° C. and a pressure of 300 MPa for three times.

[0132] (2) reflux extraction of the guava obtained in step (1) for 20 minutes;

[0133] (3) The product obtained by reflux extraction was centrifuged at 5000 rpm for 10 min, the supernatant was filtered through a 200 nm filter membrane, concentrated to 5 wt% at 60° C. and 110 mbar, sterilized, and dried to obtain the product.

[0134] Example 1

[0135] This embodiment provides a composition with anti-inflammatory and anti-aging effects, which comprises, by mass, 50 parts of the Cornus officinalis extract obtained in Preparation Example 1-1, 30 parts of the Prunus mume extract obtained in Preparation Example 2-1, and 10 parts of the Psidium guajava extract obtained in Preparation Example 3-1.

[0136] The preparation method thereof is as follows: physically mixing the preparation raw materials to obtain the product.

[0137] Example 2

[0138] This embodiment provides a composition with anti-inflammatory and anti-aging effects, which comprises, by mass, 10 parts of the Cornus officinalis extract obtained in Preparation Example 1-2, 20 parts of the Prunus mume extract obtained in Preparation Example 2-2, and 50 parts of the Psidium guajava extract obtained in Preparation Example 3-2.

[0139] The preparation method is as in Example 1.

[0140] Example 3

[0141] This embodiment provides a composition with anti-inflammatory and anti-aging effects, which comprises, by mass, 30 parts of the Cornus officinalis extract obtained in Preparation Example 1-3, 50 parts of the Prunus mume extract obtained in Preparation Example 2-3, and 30 parts of the Psidium guajava extract obtained in Preparation Example 3-3.

[0142] The preparation method is as in Example 1.

[0143] Examples 4-8

[0144] This example provides five compositions with anti-inflammatory and anti-aging effects, which differ from Example 1 only in that the Cornus officinalis extract obtained in Preparation Example 1-1 is replaced in equal amounts by the Cornus officinalis extracts obtained in Preparation Example 1-4, Preparation Example 1-5, Preparation Example 1-6, Preparation Example 1-7, and Preparation Example 1-8, while the other ingredients and contents remain unchanged.

[0145] The preparation method is as in Example 1.

[0146] Examples 9-12

[0147] This example provides four compositions with anti-inflammatory and anti-aging effects, which differ from Example 1 only in that the five-fingered ginseng extract obtained in Preparation Example 2-1 is replaced in equal amounts by the five-fingered ginseng extracts obtained in Preparation Example 2-4, Preparation Example 2-5, Preparation Example 2-6, and Preparation Example 2-7, while the other ingredients and contents remain unchanged.

[0148] The preparation method is as in Example 1.

[0149] Comparative Example 1

[0150] This comparative example provides a composition with anti-inflammatory and anti-aging effects, which differs from Example 1 only in that it does not contain Cornus officinalis extract, and its reduced mass is proportionally distributed to the mass of the Prunus mume extract and the Psidium guajava extract.

[0151] The preparation method is as in Example 1.

[0152] Comparative Example 2

[0153] This comparative example provides a composition with anti-inflammatory and anti-aging effects, which differs from Example 1 only in that it does not contain the Prunus mume extract, and its reduced mass is proportionally distributed to the mass of the Cornus officinalis extract and the Psidium guajava extract.

[0154] The preparation method is as in Example 1.

[0155] Comparative Example 3

[0156] This comparative example provides a composition with anti-inflammatory and anti-aging effects, which differs from Example 1 only in that it does not contain guava extract, and its reduced mass is proportionally distributed to the mass of the quinquefolia extract and the cornus officinalis extract.

[0157] The preparation method is as in Example 1.

[0158] Test Example 1

[0159] DPPH free radical scavenging experiment

[0160] Experimental methods:

[0161] The DPPH solution was dissolved in anhydrous ethanol to a concentration of 0.1 mg / mL. A 0.5 mg / mL vitamin C standard (positive control) and a sample solution to be tested were simultaneously prepared. 150 μL of the DPPH alcohol solution and 150 μL of the sample solution were mixed evenly, reacted in the dark at room temperature for 30 minutes, and shaken to mix well. The absorbance was measured at 517 nm. Each group was measured three times on average, and the average was taken. The DPPH radical scavenging rate of the composite sample was calculated according to the following formula. The results are shown in Table 1.

[0162] Sample group: sample solution 150μL + DPPH alcohol solution 150μL

[0163] Sample blank group: DPPH solution 150μL + sample solvent 150μL

[0164] Control group: 150 μL sample solution + 150 μL anhydrous ethanol

[0165] Blank control group: 150 μL of anhydrous ethanol + 150 μL of sample solvent

[0166]

[0167] Table 1

[0168] Group Clearance rate (%) Example 1 98.71 Example 2 96.15 Example 3 95.61 Example 4 85.63 Example 5 89.37 Example 6 88.58 Example 7 89.08 Example 8 88.64 Example 9 87.45 Example 10 90.64 Example 11 82.18 Example 12 81.64 Comparative Example 1 58.25 Comparative Example 2 80.67 Comparative Example 3 68.72 Preparation Example 1-1 81.13 Preparation Example 2-1 38.48 Preparation Example 3-1 71.85 Positive control 98.74

[0169] It can be seen from the data results in Table 1 that the composition described in the present application has a good DPPH free radical scavenging effect. The preparation method of Cornus officinalis extract and Eleutherodactyl extract will also affect the effect of the composition. In addition, Cornus officinalis extract, Eleutherodactyl extract and Psidium guajava extract have a certain synergistic effect in antioxidant efficacy, and none of them can be missing.

[0170] Test Example 2

[0171] Collagenase activity inhibition assay

[0172] Experimental methods:

[0173] Use Tris-HCl buffer (pH = 7.5 ± 0.2, containing 5 mmol / mL CaCl2) to prepare 1 mg / mL tetracycline hydrochloride (positive control) and the sample solution to be tested; prepare 0.5 mg / mL substrate FALGPA buffer solution; prepare 0.2 mg / mL type I collagenase Tris-HCl solution.

[0174] Blank, control, sample, and sample-blank groups were set up. 140 μL of 0.2 mg / mL enzyme solution was added to the control and sample groups, and an equal volume of buffer was added to the blank and sample-blank groups. After incubation at 37°C for 20 minutes, 40 μL of the substrate FALGPA solution was added to each group. After incubation at 37°C for 20 minutes, the absorbance of each component at 330 nm was measured. Three measurements were performed for each group, and the average value was calculated. The collagenase inhibition rate was calculated according to the following formula. The results are shown in Table 2.

[0175] Sample blank group: sample solution 60μL + buffer salt 140μL + substrate 40μL

[0176] Sample group: sample solution 60 μL + collagenase 140 μL + substrate 40 μL

[0177] Control group: 60 μL buffer salt + 140 μL collagenase + 40 μL substrate

[0178] Blank group: 200 μL buffer + 40 μL substrate

[0179]

[0180] Table 2

[0181]

[0182]

[0183] It can be seen from the data results in Table 2 that the composition described in the present application has a good collagenase inhibitory effect. The preparation method of Cornus officinalis extract and Eleutherodactyl extract will also affect the effect of the composition, and Cornus officinalis extract, Eleutherodactyl extract and Psidium guajava extract have a certain synergistic effect in this effect, and none of them can be missing.

[0184] Test Example 3

[0185] AGEs inhibition experiment

[0186] Experimental methods:

[0187] A 1 mg / mL solution of aminoguanidine hydrochloride (positive control) and the test sample were prepared in phosphate buffer (50 mmol / L, pH 7.4). 1 mL each of the test sample, 0.8 mg / mL bovine serum albumin (BSA) solution, 200 mM glucose solution (GLU), and PBS buffer were added, and the mixture was heated at 60°C for 24 hours. Fluorescent AGEs were measured using a fluorescence microplate reader with an excitation wavelength of 370 nm and an emission wavelength of 420 nm. The fluorescent AGE content was expressed as fluorescence intensity (AU). The fluorescence intensity of each group was measured before and after the reaction. Each group was measured three times, and the average value was taken. The sample's inhibition rate on fluorescent AGE formation was calculated using the following formula, and the results are shown in Table 3.

[0188] Sample set: sample solution 1mL + buffer 1mL + BSA 1mL + GLU 1mL

[0189] Blank control group: PBS buffer 2 mL + BSA 1 mL + GLU 1 mL

[0190]

[0191] Where:

[0192] A0 is the initial fluorescence intensity of the blank control group;

[0193] A1 is the initial fluorescence intensity of the experimental group;

[0194] B0 Fluorescence intensity of blank control group after reaction;

[0195] B1 Fluorescence intensity of experimental group after reaction.

[0196] Table 3

[0197] Group IC50 value (μg / mL) Example 1 167.72 Example 2 178.64 Example 3 169.72 Example 4 203.93 Example 5 212.35 Example 6 226.80 Example 7 279.75 Example 8 208.32 Example 9 370.68 Example 10 324.09 Example 11 305.47 Example 12 315.09 Comparative Example 1 394.56 Comparative Example 2 716.29 Comparative Example 3 535.2 Preparation Example 1-1 723.14 Preparation Example 2-1 285.49 Preparation Example 3-1 450.28 Positive control 488.45

[0198] As can be seen from the data results in Table 3, the composition described in the present application has a good inhibitory effect on the formation of fluorescent AGEs. The preparation methods of Cornus officinalis extract and Eleutherodactyl extract will also affect the effect of the composition. In addition, Cornus officinalis extract, Eleutherodactyl extract and Psidium guajava extract have a certain synergistic effect in anti-glycation effect, and none of them can be missing.

[0199] Test Example 4

[0200] LPS-induced anti-inflammatory experiment in macrophage RAW264.7 cells

[0201] Test method:

[0202] According to the results of the CCK-8 assay, the test concentration of each sample group was selected as 125 μg / mL to determine the anti-inflammatory efficacy of macrophage RAW264.7. A blank control group, a model group, a positive control group, and a sample group were set up. Complete culture medium was added to each well of the blank control group, 5 μM dexamethasone sodium acetate diluted with complete culture medium was added to each well of the positive control group, and samples diluted with complete culture medium were added to each well of the sample group. Three replicates were set up for each group and incubated in a 37°C, 5% CO2 incubator for 2 hours. Except for the blank control group, LPS was added to each well at a final concentration of 1 μg / mL and incubated in a 37°C, 5% CO2 incubator for 24 hours. The cell supernatant was collected by centrifugation in a low-temperature centrifuge and tested according to the instructions of the NO detection kit. The NO content in the collected cell supernatant was detected. The experimental data are shown in Table 4.

[0203] Sample group: samples diluted in complete medium and LPS;

[0204] Model group: complete culture medium and LPS;

[0205] Positive control group: 5 μM dexamethasone sodium acetate and LPS diluted in complete culture medium;

[0206] Blank control group: complete culture medium only.

[0207] Table 4

[0208]

[0209]

[0210] It can be seen from the data results in Table 4 that the composition described in the present application has a good inhibitory effect on the secretion of NO. The preparation method of Cornus officinalis extract and Eleutherodactyl extract will also affect the effect of the composition. In addition, Cornus officinalis extract, Eleutherodactyl extract and Psidium guajava extract have a certain synergistic effect in anti-inflammatory effect, and none of them can be missing.

[0211] Test Example 5

[0212] Zebrafish microcirculation improvement experiment

[0213] Zebrafish strain: wild-type AB strain, 30 zebrafish per experimental group, cultured in a 28°C incubator. Experimental methods:

[0214] Based on the MTC assay results, a 100 μg / mL concentration was selected for zebrafish microcirculatory improvement experiments. 48 hpf zebrafish of consistent developmental status were randomly assigned to 6-well plates, 30 per well. A single concentration group (100 μg / mL) was established within the sample group, with aspirin as the active agent. A normal control group and a model control group were also established. Except for the normal control group, all other experimental groups were treated with 0.9% sodium chloride in water to establish a zebrafish microcirculatory impairment model. 3 mL of the test substance solution was then added to each well. After incubation at 28°C for 24 hours, 10 zebrafish were randomly selected from each experimental group and placed under a blood flow analyzer to record blood flow videos. The blood flow analyzer was used to analyze the mid-abdomen, where the zebrafish blood vessels were most clearly visible and blood flow was highest. Blood flow velocity (V) was measured for each zebrafish. Blood flow velocity was measured using the blood flow analyzer. The formula for improving sample blood flow velocity is as follows, and the results are shown in Table 5.

[0215] Normal control group: containing zebrafish and standard dilution aqueous solution;

[0216] Model group: containing zebrafish prepared with 0.9% NaCl solution;

[0217] Sample group: Contains the test substance prepared with 0.9% NaCl solution and zebrafish.

[0218]

[0219] Table 5

[0220]

[0221] Note: Compared with the blank group, ### p<0.001, compared with the model group, * p<0.05, *** p<0.001

[0222] It can be seen from the data results in Table 5 that the composition described in the present application has a significant promoting effect on the blood flow rate of zebrafish. The preparation method of Cornus officinalis extract and Eleutherodactyl extract will also affect the effect of the composition. Cornus officinalis extract, Eleutherodactyl extract and Psidium guajava extract have a certain synergistic effect in improving microcirculation, and none of them can be missing.

[0223] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a composition having anti-inflammatory and anti-aging efficacy and its application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent replacements for various raw materials in the product of the present invention, addition of auxiliary ingredients, and selection of specific methods, etc., are all within the scope of protection and disclosure of the present invention.

[0224] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0225] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A composition with anti-inflammatory and anti-aging effects, characterized in that: The composition comprises, by weight, 10-50 parts of Cornus officinalis extract, 20-60 parts of Prunus mume extract, and 10-50 parts of Psidium guajava extract; The cornus officinalis extract is prepared by a method comprising the following steps: (1) Mixing Cornus officinalis with water and pre-treating with ultrahigh pressure microfluidization; (2) mixing the pretreated Cornus officinalis with an enzyme for enzymolysis to obtain an enzymatic hydrolyzate; (3) The enzymatic hydrolysate is filtered through a sieve, a ceramic membrane, and an ultrafiltration membrane in sequence, and then concentrated and dried to obtain the product; The five-fingered ginseng extract is prepared by a method comprising the following steps: (1) Mix the five-fingered peach with water, treat at 110-130 °C and 0.1-0.2 MPa for 15-30 min, and extract under ultrasound to obtain an extract; (2) Filter the extract, concentrate it, and dry it to obtain the product; The guava extract is prepared by a method comprising the following steps: (1) Mixing guava with water and pre-treating with ultrahigh pressure microfluidization; (2) subjecting the pretreated guava to reflux extraction to obtain an extract; (3) Centrifuge the extract to obtain the supernatant, filter the supernatant, concentrate it, and dry it to obtain the product.

2. The composition with anti-inflammatory and anti-aging effects according to claim 1, characterized in that The ultra-high pressure microfluidic pretreatment is performed at a pressure of 100-300 MPa, a temperature of 60-80° C., and a number of times of 2-6 times.

3. The composition with anti-inflammatory and anti-aging effects according to claim 1, characterized in that The enzyme is a combination of cellulase and pectinase, and the amount of enzyme added is 2-5% of the weight of Cornus officinalis; The enzymatic hydrolysis temperature is 35-55° C., the pH is 3-5.5, and the time is 20-30 min.

4. The composition with anti-inflammatory and anti-aging effects according to claim 1, characterized in that The ultrasonic temperature is 60-80°C, the frequency is 30-40 kHz, the time is 15-30 minutes, and the number of times is 1-3 times.

5. Use of the composition with anti-inflammatory and anti-aging effects according to any one of claims 1 to 4 in the preparation of a product with anti-inflammatory, anti-aging, antioxidant or anti-glycation effects.

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