Phospholipid private care gel and preparation method thereof

Abstract

The invention discloses a phospholipid private care gel and a preparation method thereof, and the phospholipid private care gel is prepared from the following components in parts by weight: 5 to 30 parts of phospholipid, 0.5 to 3 parts of hydroxyethyl cellulose, 0.2 to 1.5 parts of raspberry ketone, 0.1 to 0.5 part of ethylhexylglycerin, 0.1 to 0.5 part of cortex magnoliae officinalis extract, 2 to 6 parts of propylene glycol and 50 to 90 parts of deionized water. The phospholipid private care gel prepared by the invention is safe, healthy and mild, has a strong permeation effect, can quickly repair private mucosa and effectively inhibit bacteria at female private parts, and has the effects of inhibiting bacteria and diminishing inflammation. According to the method, the soybean is used as the raw material, the mixed enzyme is adopted for primary dissociation to form the micromolecular plant protein, then the inoculated strain is utilized for fermentation, and then the precipitation liquid is utilized for precipitation to obtain the phospholipid, so that the production cost is relatively low, and the yield is very high.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technology, and in particular to a phospholipid intimate care gel and a preparation method thereof. Background Art

[0002] Gynecological diseases, also known as gynecological diseases, are a general term for common diseases of the female reproductive system. With the development of the economy and the improvement of living standards, people's work and life pressures are increasing, their daily routines are extremely irregular, their biological clocks are disrupted, their body resistance decreases, their private immunity continues to decrease, and harmful bacteria take advantage of the situation to cause various gynecological inflammations. An ideal female private sterilization and care product should have a broad spectrum of anti-vaginal bacteria activity, good solubility and dispersibility, safety, harmlessness to the human body, and ease of use. However, there are many types of existing sterilization products, which cause certain damage to the female vaginal mucosa. In the prior art, some varieties of gynecological vaginal diseases are basically antibiotic products that are mainly disinfected and killed. While curing inflammation, they also kill the beneficial bacteria that protect the human body. Once there is no protection of the beneficial bacteria, the harmful bacteria will spread more wantonly, and more gynecological diseases will follow. Summary of the invention

[0003] In order to overcome the above-mentioned shortcomings, the purpose of the present invention is to provide a phospholipid private care gel and a preparation method thereof. The phospholipid private care gel is mild and non-irritating and has a high antibacterial rate.

[0004] In order to achieve the above purpose, the technical solution adopted by the present invention is: a phospholipid private care gel, which includes, by weight: 5 to 30 parts of phospholipids, 0.5 to 3 parts of hydroxyethyl cellulose, 0.2 to 1.5 parts of raspberry ketone, 0.1 to 0.5 parts of ethylhexylglycerin, 0.1 to 0.5 parts of magnolia bark extract, 2 to 6 parts of propylene glycol, and 50 to 90 parts of deionized water.

[0005] Exemplarily, the phospholipids are in the range of 5 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts or any two of them; the hydroxyethyl cellulose is in the range of 0.5 parts, 1 parts, 2 parts, 3 parts or any two of them; the raspberry ketone is in the range of 0.2 parts, 0.5 parts, 1.0 parts, 1.5 parts or any two of them; the ethylhexylglycerin is in the range of 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts or any two of them; the Magnolia officinalis bark extract is in the range of 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts or any two of them; the propylene glycol is in the range of 2 parts, 3 parts, 4 parts, 5 parts, 6 parts or any two of them; and the deionized water is in the range of 50 parts, 60 parts, 70 parts, 80 parts, 90 parts or any two of them.

[0006] Compared with the prior art, the phospholipid private care gel prepared by the present invention has a strong repair ability for the private mucosa, avoids the dryness and irritation caused by bactericides, has the characteristics of safety, health, and mildness, has a strong penetration effect, can effectively inhibit bacteria in the female private parts, and has the effect of antibacterial and anti-inflammatory.

[0007] Furthermore, the preparation method of phospholipids comprises the following steps: S1. Grind soybeans into 300-500 mesh soybean powder; S2. Put soybean powder into a fermentation tank, add purified water, adjust the pH value to 4.0-6.0, stir and heat; S3, adding the first group of mixed enzymes into the fermentation tank, stirring and enzymatically cutting; S4, adding a carbon source and a nitrogen source into the fermentation tank, stirring and heating; S5, placing the strain in a fermentation tank for fermentation; S6. Add precipitating liquid into the fermentation tank for stirring and precipitation, filter out the precipitate, and obtain phospholipids.

[0008] Further, in S2, the pH value is adjusted to 4.0-6.0 with citric acid and sodium hydroxide, and the mixture is heated to 85-95°C for sterilization for 30-50 minutes with stirring, and then cooled to 30-40°C with stirring.

[0009] Furthermore, in S3, the first group of mixed enzymes includes protease, lipase, pectinase, cellulase and amylase, and their mass ratios are 1:1.5:0.5:0.5:1.5, respectively, and their activities are 1.2 million u / g, 1.5 million u / g, 800,000 u / g, 2 million u / g and 1.3 million u / g, respectively. The total mass of the first group of mixed enzymes accounts for 0.1-0.8% of the mass of soybean flour.

[0010] Furthermore, in S4, the carbon sources are sucrose and glucose, wherein the mass ratio of sucrose to glucose is 2:3, and the total mass of the carbon sources accounts for 0.5-1.2% of the mass of the soybean powder; the nitrogen sources include peptone, casein hydrolysate, yeast extract, beef extract, and urea, wherein the mass ratios are 2:0.5:1.5:0.5:0.5, respectively, and the total mass of the nitrogen sources accounts for 1.3-2.0% of the mass of the soybean powder.

[0011] Further, in S4, after adding the carbon source and the nitrogen source, stirring is continued for 40 to 60 minutes, the stirring speed is 100 to 300 r / min and the mixture is stirred evenly, and the mixture is heated to 80 to 85° C. and kept warm for 30 to 40 minutes for thorough sterilization, and then stirred and cooled to 30 to 40° C.

[0012] Furthermore, in step S5, the bacterial species are bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus, and the mass ratio of bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus is 1:2:2; wherein the total mass of the bacterial species is 0.02% to 0.05% of the total mass of soybean powder, purified water, carbon source and nitrogen source.

[0013] Furthermore, in step S5, the fermentation time is 12 to 24 hours, and the ventilation volume is maintained at 0.4 to 1.2 vvm; the sterilization treatment is stirring and heating to 80 to 85° C., and keeping warm for 30 to 40 minutes; during the fermentation process, the pH value is controlled to 4.0 to 6.0 with a 2% sodium hydroxide solution.

[0014] Further, in S6, the precipitating liquid is 95% ethanol.

[0015] Furthermore, step S6 also includes the following steps: washing the precipitate with a cleaning solution and then putting it into a clean fermentation tank, adding purified water and stirring evenly, wherein the cleaning solution is 95% ethanol, and the mass ratio of the precipitate to the purified water is 1: (40-60).

[0016] The preparation method of phospholipid private care gel comprises the following steps: Step 1: Add deionized water into the main pot and heat it to 80℃~85℃; Step 2: Mix hydroxyethyl cellulose and raspberry ketone in another measuring container and mix them completely to dissolve, then add them to the main pot in step 1 and mix; Step 3, add ethylhexylglycerin and stir until completely dissolved, then add the phospholipid phase and homogenize for 5 minutes, keep warm at 80℃~85℃ for 20 minutes, and then add Magnolia officinalis bark extract; Step 4: When the temperature of the main pot drops to 40℃~45℃, add propylene glycol, stir evenly, take samples for testing (semi-finished product 1), test the material for qualified, discharge (semi-finished product 2), transfer the material. Sterilize the packaging material, fill and package, and obtain the intimate care gel.

[0017] The beneficial effects of the present invention are: 1) The phospholipid intimate care gel prepared by the present invention is safe, healthy, mild, has a strong penetration effect, can quickly repair the intimate mucosa and effectively inhibit bacteria in the female intimate parts, and has the effect of inhibiting bacteria and reducing inflammation.

[0018] 2) The present invention uses soybean as raw material, uses a mixed enzyme to initially dissociate into small molecule plant protein, then uses the accession strain for fermentation, and then uses the precipitation liquid for precipitation to obtain phospholipids. The production cost is relatively low and the output rate is very high. DETAILED DESCRIPTION

[0019] In order to make the above-mentioned purpose, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0020] Except as shown in the operating examples or otherwise indicated, all numbers used in the specification and claims to indicate the amount of ingredients, physicochemical properties, etc. are understood to be adjusted by the term "about" in all cases. Therefore, unless otherwise indicated, the numerical parameters listed in the above specification and the attached claims are approximate values, and those skilled in the art can use the teachings disclosed herein to seek to obtain the desired properties and appropriately change these approximate values. The use of numerical ranges expressed as endpoints includes all numbers within the range and any range within the range, for example, 1 to 5 includes 1, 1.1, 1.3, 1.5, 2, 2.75, 3, 3.80, 4 and 5, etc.

[0021] A phospholipid intimate care gel in this embodiment includes, by weight: 5 to 30 parts of phospholipids, 0.5 to 3 parts of hydroxyethyl cellulose, 0.2 to 1.5 parts of raspberry ketone, 0.1 to 0.5 parts of ethylhexylglycerin, 0.1 to 0.5 parts of magnolia bark extract, 2 to 6 parts of propylene glycol, and 50 to 90 parts of deionized water.

[0022] The preparation method of phospholipids comprises the following steps: S1. Grind soybeans into 300-500 mesh soybean powder.

[0023] Select high-quality soybeans, wash them with clean water, and use a spectrum cleaner to wash them twice to obtain clean soybeans; put the clean soybeans into an air-drying room to dry them naturally for 24 hours, grind the clean soybeans with a grinder, and sieve them to obtain soybean powder with a mesh size of 300 to 500.

[0024] S2. Put soybean powder into a fermentation tank, add purified water, adjust the pH value to 4.0-6.0 with citric acid and sodium hydroxide, stir and heat to 85-95℃ for sterilization for 30-50min, and stir and cool to 30-40℃.

[0025] S3. Add the first set of mixed enzymes into the fermentation tank and stir for enzyme cutting.

[0026] In some embodiments, the first group of mixed enzymes includes protease, lipase, pectinase, cellulase, and amylase, and their mass ratios are 1:1.5:0.5:0.5:1.5, respectively, and their activities are 1.2 million u / g, 1.5 million u / g, 800,000 u / g, 2 million u / g, and 1.3 million u / g, respectively. The total mass of the first group of mixed enzymes accounts for 0.1-0.8% of the mass of soybean flour.

[0027] S4. Add the carbon source and nitrogen source to the fermentation tank, continue stirring for 40 to 60 minutes at a stirring speed of 100 to 300 r / min, stir evenly, heat to 80 to 85°C, keep warm for 30 to 40 minutes for thorough sterilization, and then stir and cool to 30 to 40°C.

[0028] In some embodiments, the carbon source is sucrose and glucose, wherein the mass ratio of sucrose to glucose is 2:3, and the total mass of the carbon source accounts for 0.5-1.2% of the mass of the soybean flour.

[0029] In some embodiments, the nitrogen source includes peptone, casein hydrolysate, yeast extract, beef extract, and urea in a mass ratio of 2:0.5:1.5:0.5:0.5, respectively, and the total mass of the nitrogen source accounts for 1.3-2.0% of the mass of the soybean flour.

[0030] S5. Place the strain into a fermentation tank for fermentation and sterilization.

[0031] In some embodiments, the bacterial strains are bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus, and the mass ratio of bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus is 1:2:2; wherein the total mass of the bacterial strains is 0.02% to 0.05% of the total mass of soybean powder, purified water, carbon source and nitrogen source.

[0032] In some embodiments, the fermentation time is 12 to 24 hours, and the ventilation volume is maintained at 0.4 to 1.2 vvm; the sterilization treatment is stirred and heated to 80 to 85°C, and kept warm for 30 to 40 minutes; during the fermentation process, a 2% sodium hydroxide solution is used to control the pH value to 4.0 to 6.0.

[0033] S6. Add precipitating liquid into the fermentation tank for stirring and precipitation, filter out the precipitate, and obtain phospholipids.

[0034] In some embodiments, the following steps are also included: the precipitate is washed with a cleaning solution and then put into a clean fermentation tank, and purified water is added and stirred evenly, wherein the cleaning solution is 95% ethanol, and the mass ratio of the precipitate to purified water is 1: (40-60).

[0035] A method for preparing a private care gel comprises the following steps: Step 1: Add 50-90 parts of deionized water into the main pot and heat to 80℃~85℃; Step 2: Mix 0.5-3 parts of hydroxyethyl cellulose and 0.2-1.5 parts of raspberry ketone, and then add them into the deionized water in step 1 and mix; Step 3, add 0.1-0.5 parts of ethylhexylglycerin and stir until completely dissolved, then add 5-30 parts of phospholipids, keep warm at 80℃-85℃ for 20 minutes, then add 0.1-0.5 parts of Magnolia officinalis bark extract; Step 4: When the temperature drops to 40°C to 45°C, add 2 to 6 parts of propylene glycol, stir evenly, take samples for testing, and obtain an intimate care gel.

[0036] The following examples describe the disclosure of the present invention in more detail, and these examples are intended for illustrative purposes only, as various modifications and variations within the scope of the disclosure of the present invention will be apparent to those skilled in the art. Unless otherwise stated, all parts, percentages, and ratios reported in the following examples are by weight, and all reagents used in the examples are commercially available or synthesized according to conventional methods and can be used directly without further processing, and the instruments used in the examples are commercially available.

[0037] Preparation Example 1 A method for preparing phospholipids in this embodiment comprises the following steps: S1. Grind soybeans into 300-500 mesh soybean powder.

[0038] Select high-quality soybeans, perform preliminary cleaning with clean water, and perform secondary cleaning with a spectrum cleaning machine to obtain clean soybeans; put the clean soybeans into an air-drying room for natural air drying for 24 hours, grind the clean soybeans with a grinder, and sieve to obtain soybean powder with a mesh size of 300 to 500.

[0039] S2. Put soybean powder into a fermentation tank, add purified water, adjust the pH value to 5 with citric acid and sodium hydroxide, stir and heat to 85℃ for sterilization for 50 minutes, and stir and cool to 30℃.

[0040] S3. Add the first set of mixed enzymes into the fermentation tank and stir for enzyme cutting.

[0041] The first group of mixed enzymes includes protease, lipase, pectinase, cellulase and amylase, and their mass ratios are 1:1.5:0.5:0.5:1.5, respectively, and their activities are 1.2 million u / g, 1.5 million u / g, 800,000 u / g, 2 million u / g and 1.3 million u / g, respectively. The total mass of the first group of mixed enzymes accounts for 0.5% of the mass of soybean flour.

[0042] S4. Add the carbon source and nitrogen source into the fermentation tank, continue stirring for 50 minutes, stir evenly at a speed of 200 r / min, heat to 80°C, keep warm for 40 minutes for thorough sterilization, and then cool to 40°C with stirring.

[0043] The carbon sources are sucrose and glucose, wherein the mass ratio of sucrose to glucose is 2:3, and the total mass of the carbon sources accounts for 1.0% of the mass of the soybean powder.

[0044] The nitrogen sources include peptone, casein hydrolyzate, yeast extract, beef extract and urea in a mass ratio of 2:0.5:1.5:0.5:0.5, respectively. The total mass of the nitrogen sources accounts for 1.5% of the mass of the soybean powder.

[0045] S5. The bacteria were inoculated into a fermentation tank for fermentation for 20 h, and the ventilation volume was maintained at 0.8 vvm. Sterilization treatment was performed by stirring and heating to 80°C and keeping warm for 40 min. During the fermentation process, the pH value was controlled to 5.0 with 2% sodium hydroxide solution.

[0046] The bacterial strains are bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus, and the mass ratio of bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus is 1:2:2; the total mass of the bacterial strains is 0.04% of the total mass of soybean powder, purified water, carbon source and nitrogen source.

[0047] S6. Add precipitating liquid into the fermentation tank for stirring and precipitation, filter out the precipitate, wash the precipitate with cleaning liquid, put it into a clean fermentation tank, add purified water and stir evenly, wherein the cleaning liquid is 95% ethanol, and the mass ratio of precipitate to purified water is 1:50. Filter out the precipitate to obtain phospholipids.

[0048] Preparation Example 2 A method for preparing phospholipids in this embodiment comprises the following steps: S1. Grind soybeans into 300-500 mesh soybean powder.

[0049] Select high-quality soybeans, perform preliminary cleaning with clean water, and perform secondary cleaning with a spectrum cleaning machine to obtain clean soybeans; put the clean soybeans into an air-drying room for natural air drying for 24 hours, grind the clean soybeans with a grinder, and sieve to obtain soybean powder with a mesh size of 300 to 500.

[0050] S2. Put soybean powder into a fermentation tank, add purified water, adjust the pH value to 4 with citric acid and sodium hydroxide, stir and heat to 95℃ for sterilization for 30 minutes, and stir and cool to 30℃.

[0051] S3. Add the first set of mixed enzymes into the fermentation tank and stir for enzyme cutting.

[0052] The first group of mixed enzymes includes protease, lipase, pectinase, cellulase and amylase, and their mass ratios are 1:1.5:0.5:0.5:1.5, respectively, and their activities are 1.2 million u / g, 1.5 million u / g, 800,000 u / g, 2 million u / g and 1.3 million u / g, respectively. The total mass of the first group of mixed enzymes accounts for 0.5% of the mass of soybean flour.

[0053] S4. Add the carbon source and nitrogen source into the fermentation tank, continue stirring for 40 minutes, stir evenly at a speed of 300 r / min, heat to 85°C, keep warm for 30 minutes for thorough sterilization, and then cool to 40°C with stirring.

[0054] The carbon sources are sucrose and glucose, wherein the mass ratio of sucrose to glucose is 2:3, and the total mass of the carbon sources accounts for 0.5% of the mass of the soybean powder.

[0055] The nitrogen sources include peptone, casein hydrolyzate, yeast extract, beef extract and urea in a mass ratio of 2:0.5:1.5:0.5:0.5, respectively. The total mass of the nitrogen sources accounts for 1.3% of the mass of the soybean powder.

[0056] S5. The bacteria were inoculated into a fermentation tank for fermentation for 12 hours, and the ventilation volume was maintained at 1.2 vvm. Sterilization treatment was performed by stirring and heating to 85°C and keeping warm for 30 minutes. During the fermentation process, the pH value was controlled to 4.0 with 2% sodium hydroxide solution.

[0057] The bacterial strains are bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus, and the mass ratio of bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus is 1:2:2; the total mass of the bacterial strains is 0.02% of the total mass of soybean powder, purified water, carbon source and nitrogen source.

[0058] S6. Add precipitating liquid into the fermentation tank for stirring and precipitation, filter out the precipitate, wash the precipitate with cleaning liquid, put it into a clean fermentation tank, add purified water and stir evenly, wherein the cleaning liquid is 95% ethanol, and the mass ratio of precipitate to purified water is 1:40. Filter out the precipitate to obtain phospholipids.

[0059] Preparation Example 3 A method for preparing phospholipids in this embodiment comprises the following steps: S1. Grind soybeans into 300-500 mesh soybean powder.

[0060] Select high-quality soybeans, perform preliminary cleaning with clean water, and perform secondary cleaning with a spectrum cleaning machine to obtain clean soybeans; put the clean soybeans into an air-drying room for natural air drying for 24 hours, grind the clean soybeans with a grinder, and sieve to obtain soybean powder with a mesh size of 300 to 500.

[0061] S2. Put soybean powder into a fermentation tank, add purified water, adjust the pH value to 6 with citric acid and sodium hydroxide, stir and heat to 85℃ for sterilization for 50 minutes, and stir and cool to 40℃.

[0062] S3. Add the first set of mixed enzymes into the fermentation tank and stir for enzyme cutting.

[0063] The first group of mixed enzymes includes protease, lipase, pectinase, cellulase and amylase, and their mass ratios are 1:1.5:0.5:0.5:1.5, respectively, and their activities are 1.2 million u / g, 1.5 million u / g, 800,000 u / g, 2 million u / g and 1.3 million u / g, respectively. The total mass of the first group of mixed enzymes accounts for 0.8% of the mass of soybean flour.

[0064] S4. Add the carbon source and nitrogen source into the fermentation tank, continue stirring for 60 minutes, stir evenly at a speed of 100 r / min, heat to 80°C, keep warm for 40 minutes for thorough sterilization, and then cool to 30°C with stirring.

[0065] The carbon sources are sucrose and glucose, wherein the mass ratio of sucrose to glucose is 2:3, and the total mass of the carbon sources accounts for 1.2% of the mass of soybean powder.

[0066] The nitrogen sources include peptone, casein hydrolyzate, yeast extract, beef extract and urea in a mass ratio of 2:0.5:1.5:0.5:0.5, respectively. The total mass of the nitrogen sources accounts for 2.0% of the mass of the soybean powder.

[0067] S5. The bacteria were inoculated into a fermentation tank for fermentation for 24 hours, and the ventilation volume was maintained at 0.4 vvm. Sterilization treatment was performed by stirring and heating to 80°C and keeping warm for 40 minutes. During the fermentation process, the pH value was controlled to 6.0 with 2% sodium hydroxide solution.

[0068] The bacterial strains are bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus, and the mass ratio of bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus is 1:2:2; the total mass of the bacterial strains is 0.05% of the total mass of soybean powder, purified water, carbon source and nitrogen source.

[0069] S6. Add precipitating liquid into the fermentation tank for stirring and precipitation, filter out the precipitate, wash the precipitate with cleaning liquid, put it into a clean fermentation tank, add purified water and stir evenly, wherein the cleaning liquid is 95% ethanol, and the mass ratio of precipitate to purified water is 1:60. Filter out the precipitate to obtain phospholipids.

[0070] Example 1 A method for preparing a private care gel comprises the following steps: Step 1: Add 70 parts of deionized water into the main pot and heat to 80°C; Step 2: Mix 1.5 parts of hydroxyethyl cellulose and 1.0 parts of raspberry ketone, and add them to the deionized water in step 1 and mix; Step 3, add 0.3 parts of ethylhexylglycerol and stir until completely dissolved, then add 20 parts of the phospholipid prepared in Preparation Example 1, keep warm at 80°C for 20 minutes, then add 0.3 parts of Magnolia officinalis bark extract; Step 4: When the temperature drops to 40°C, add 4 parts of propylene glycol, stir evenly, take samples for testing, and obtain the intimate care gel.

[0071] Example 2 A method for preparing a private care gel comprises the following steps: Step 1: Add 50 parts of deionized water into the main pot and heat to 85°C; Step 2: Mix 0.5 parts of hydroxyethyl cellulose and 0.2 parts of raspberry ketone, and add them to the deionized water in step 1 and mix; Step 3, add 0.1 parts of ethylhexylglycerol and stir until completely dissolved, then add 20 parts of the phospholipid prepared in Preparation Example 1, keep warm at 85°C for 20 minutes, then add 0.5 parts of Magnolia officinalis bark extract; Step 4: When the temperature drops to 45°C, add 2 parts of propylene glycol, stir evenly, take samples for testing, and obtain the intimate care gel.

[0072] Example 3 A method for preparing a private care gel comprises the following steps: Step 1: Add 90 parts of deionized water into the main pot and heat to 80°C; Step 2: Mix 3 parts of hydroxyethyl cellulose and 1.5 parts of raspberry ketone, and add them to the deionized water in step 1 and mix; Step 3, add 0.5 parts of ethylhexylglycerol and stir until completely dissolved, then add 30 parts of the phospholipid prepared in Preparation Example 1, keep warm at 80°C for 20 minutes, then add 0.1 parts of Magnolia officinalis bark extract; Step 4: When the temperature drops to 40°C, add 6 parts of propylene glycol, stir evenly, take samples for testing, and obtain the intimate care gel.

[0073] Example 4 Example 4 is basically the same as Example 1, the main difference being that the phospholipid used is prepared in Preparation Example 2.

[0074] Example 5 Example 5 is basically the same as Example 2, the main difference being that the phospholipid used is prepared in Preparation Example 2.

[0075] Example 6 Example 6 is basically the same as Example 3, the main difference being that the phospholipid used is prepared in Preparation Example 2.

[0076] Example 7 Example 7 is basically the same as Example 1, the main difference being that the phospholipid used is prepared in Preparation Example 3.

[0077] Example 8 Example 8 is basically the same as Example 2, the main difference being that the phospholipid used is prepared in Preparation Example 3.

[0078] Example 9 Example 9 is basically the same as Example 3, the main difference being that the phospholipid used is prepared in Preparation Example 3.

[0079] Comparative Example 1 Example 7 is substantially the same as Example 1, the major difference being that no phospholipid is added.

[0080] Test Example 1 Cytotoxicity test According to GB / T16886.12-2005 Biological Evaluation of Medical Devices Part 12: Sample Preparation, the extraction medium is a serum-containing culture medium, the water absorption capacity of the sample is 2ml / 0.1g (i.e., 0.1g sample absorbs 2ml of water), the extraction ratio is 3ml / 0.1g (i.e., 0.1g sample uses 3ml of extraction medium), and the extract is extracted at 37±1°C for 72±2 hours to obtain the extract. According to the prescribed method in GB / T16886.5-2003 Biological Evaluation of Medical Devices Part 5: In vitro cytotoxicity test, the results show that the cytotoxicity reaction of the phospholipid intimate care gel prepared in Examples 1-9 is less than Grade 1.

[0081] Test Example 2 Irritation test According to the method specified in GB / T16886.10-2005 Biological Evaluation of Medical Devices Part 10: Irritation and Delayed Hypersensitivity Reaction Test, the paraffin-embedded materials were stained with hematoxylin-eosin and observed under an optical microscope. The results showed that the intimate care gels prepared in Examples 1 to 9 had no visible discharge, erythema, and edema at the vaginal opening and perineum, and no inflammatory cell infiltration and no vaginal irritation reaction were observed under an optical microscope in tissue sections.

[0082] Test Example 3 Antibacterial experiment This test example conducted a 24-hour antibacterial test on the phospholipid private care gels prepared in Examples 1-9 and Comparative Example 1 to characterize the inhibitory effect of each product on private pathogens. The test method specifically refers to the "Disinfection Technical Specifications" (2002 Edition). The results are shown in Table 1.

[0083] Table 1

[0084] As shown in Table 1, the phospholipid intimate care gels obtained in Examples 1 to 9 have relatively high inhibition rates against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans.

[0085] The present invention illustrates the detailed process equipment and process flow of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned detailed process equipment and process flow, that is, it does not mean that the present invention must rely on the above-mentioned detailed process equipment and process flow to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, change of structural morphology, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0086] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0087] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

[0088] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

[0089] The above implementation modes are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with this technology to understand the content of the present invention and implement it, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A phospholipid private care gel, characterized in that: By weight, the invention comprises: 5 to 30 parts of phospholipids, 0.5 to 3 parts of hydroxyethyl cellulose, 0.2 to 1.5 parts of raspberry ketone, 0.1 to 0.5 parts of ethylhexylglycerol, 0.1 to 0.5 parts of magnolia officinalis bark extract, 2 to 6 parts of propylene glycol, and 50 to 90 parts of deionized water.

2. The phospholipid intimate care gel according to claim 1, wherein the preparation method of the phospholipid comprises the following steps: S1. Grind soybeans into 300-500 mesh soybean powder; S2. Put soybean powder into a fermentation tank, add purified water, adjust the pH value to 4.0-6.0, stir and heat; S3, adding the first group of mixed enzymes into the fermentation tank, stirring and enzymatically cutting; S4, adding a carbon source and a nitrogen source into the fermentation tank, stirring and heating; S5, placing the strain in a fermentation tank for fermentation; S6. Add precipitating liquid into the fermentation tank for stirring and precipitation, filter out the precipitate, and obtain phospholipids.

3. The phospholipid intimate care gel according to claim 2, characterized in that: In S2, the pH value is adjusted to 4.0-6.0 with citric acid and sodium hydroxide, and the mixture is heated to 85-95° C. for sterilization for 30-50 min with stirring, and then cooled to 30-40° C. with stirring; in step S6, the precipitating liquid is 95% ethanol.

4. The phospholipid intimate care gel according to claim 2, characterized in that: In S3, the first group of mixed enzymes includes protease, lipase, pectinase, cellulase, and amylase in a mass ratio of 1:1.5:0.5:0.5:1.5, and their activities are 1.2 million u / g, 1.5 million u / g, 800,000 u / g, 2 million u / g, and 1.3 million u / g, respectively. The total mass of the first group of mixed enzymes accounts for 0.1 to 0.8% of the mass of soybean flour.

5. The phospholipid intimate care gel according to claim 2, characterized in that: In S4, the carbon sources are sucrose and glucose, wherein the mass ratio of sucrose to glucose is 2:3, and the total mass of the carbon sources accounts for 0.5-1.2% of the mass of the soybean powder; the nitrogen sources include peptone, casein hydrolysate, yeast extract, beef extract, and urea, wherein the mass ratios are 2:0.5:1.5:0.5:0.5, respectively, and the total mass of the nitrogen sources accounts for 1.3-2.0% of the mass of the soybean powder.

6. The phospholipid intimate care gel according to claim 2, characterized in that: In S4, after adding the carbon source and the nitrogen source, stirring is continued for 40 to 60 minutes at a stirring speed of 100 to 300 r / min, and the mixture is heated to 80 to 85°C while stirring. After being kept warm for 30 to 40 minutes for sterilization, the mixture is cooled to 30 to 40°C while stirring.

7. The phospholipid intimate care gel according to claim 2, characterized in that: In step S5, the bacterial strains are bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus, and the mass ratio of bifidobacterium, Lactobacillus bulgaricus and Streptococcus thermophilus is 1:2:2; wherein the total mass of the bacterial strains is 0.02% to 0.05% of the total mass of soybean powder, purified water, carbon source and nitrogen source.

8. The phospholipid intimate care gel according to claim 2, characterized in that: In step S5, the fermentation time is 12 to 24 hours, and the ventilation volume is maintained at 0.4 to 1.2 vvm; the sterilization treatment is stirred and heated to 80 to 85° C., and kept warm for 30 to 40 minutes; during the fermentation process, the pH value is controlled to 4.0 to 6.0 with a 2% sodium hydroxide solution.

9. The phospholipid intimate care gel according to claim 2, characterized in that: Step S6 also includes the following steps: washing the precipitate with a cleaning solution and then putting it into a clean fermentation tank, adding purified water and stirring evenly, wherein the cleaning solution is 95% ethanol, and the mass ratio of the precipitate to the purified water is 1: (40-60).

10. The method for preparing the phospholipid intimate care gel according to any one of claims 1 to 9, characterized in that: The following steps are involved: Step 1: Add deionized water into the main pot and heat it to 80℃~85℃; Step 2, mixing hydroxyethyl cellulose and raspberry ketone, and then adding them into the deionized water in step 1 and mixing; Step 3, add ethylhexylglycerin and stir until completely dissolved, then add phospholipids, keep warm at 80℃~85℃ for 20 minutes, then add Magnolia officinalis bark extract; Step 4: When the temperature drops to 40°C to 45°C, add propylene glycol and stir evenly to obtain an intimate care gel.

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