Traditional Chinese medicine composition for treating viral pneumonia
By using Chinese medicine compositions prepared by four raw materials such as lampstick leaves, Dendrobium, Mulberry and Poria, the problems of complex and difficult quality control of existing Chinese medicine compositions have been solved, and the prescriptions have been simplified and significant effects of antiviral pneumonia have been achieved.
Patent Information
- Application Number
- CN202411966167.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-30
- Publication Date
- 2025-04-11
AI Technical Summary
The existing traditional Chinese medicine compositions used to treat viral pneumonia have problems such as complex composition, many raw materials, inconvenient materials and cumbersome preparation methods, which leads to great difficulty in quality control.
Using four raw materials: lampstick leaves, dendrobium, mulberry bark and poria, a Chinese medicine composition with simple formula and easy to obtain is prepared by decocting, concentrated thickening extract, crushing and mixing, and adding sucrose and magnesium stearate.
The Chinese medicine composition is simplified in composition, has a wide range of raw materials, is convenient for quality control, and has shown significant antiviral pneumonia effects through antiviral pneumonia zebrafish test.
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Abstract
Description
Technical Field
[0001] The present application relates to the technical field of traditional Chinese medicine, and in particular to a traditional Chinese medicine composition for treating viral pneumonia. Background Art
[0002] Viral infectious diseases account for 90% of clinical acute respiratory infections, with the upper respiratory tract being the main route. Viral pneumonia is a lung disease caused by an upper respiratory tract viral infection that spreads downward, leading to an inflammatory outbreak. The main clinical manifestations include headache, dry cough, fever, limb fatigue, body aches, and lung infiltration.
[0003] At present, Western medicine mainly adopts symptomatic treatment such as antiviral, antibacterial and antifungal treatment for viral pneumonia. Western medicine is mainly used for antipyretic, analgesic and antiviral. Although it cannot kill the virus, it can relieve symptoms such as fever and muscle aches, but the side effects are large. Some Western medicines for viral pneumonia can shorten the improvement time of clinical symptoms of pneumonia patients and effectively promote the recovery of adult patients hospitalized with viral pneumonia accompanied by lower respiratory tract infection. The clinical performance is good, but its most common clinical adverse reactions are also more serious adverse events such as elevated liver enzymes, diarrhea, rash, renal function damage and hypotension, multiple organ dysfunction syndrome, septic shock, acute kidney injury, etc. In addition, Paxlovid (namatevir / ritonavir combination tablets) that can block viral replication has side effects such as impaired taste, diarrhea, hypertension and muscle aches.
[0004] Traditional Chinese medicine is a system with unique theories and technical methods. Traditional Chinese medicine treats viral pneumonia not only by targeting the virus itself, but also by regulating the body as a whole, such as enhancing the body's immune response.
[0005] The prior art discloses a large number of traditional Chinese medicine compositions for the treatment of viral pneumonia, which have certain preventive and therapeutic effects on viral pneumonia. However, their disadvantages are that they involve a large number of raw materials, have complex formulations, are inconvenient to obtain materials, and have cumbersome preparation methods, making them inconvenient for quality control. Summary of the invention
[0006] In order to solve or partially solve the problems existing in the related art, the present application provides a traditional Chinese medicine composition for treating viral pneumonia, which has a simple formula and is easy to obtain materials, and the traditional Chinese medicine composition has a good therapeutic effect on viral pneumonia.
[0007] A traditional Chinese medicine composition for treating viral pneumonia in the present application, the effective ingredients of the traditional Chinese medicine composition are made of the following raw medicinal materials in the following weight ratio: 1000g of Alpinia scabra leaves, 500g of Dendrobium officinale, 300g of Morus alba bark, and 200g of Poria cocos.
[0008] Furthermore, pharmaceutically acceptable excipients are also included, such as sucrose, magnesium stearate and the like.
[0009] The present application also provides a method for preparing a Chinese medicine composition for treating viral pneumonia, which is prepared according to the following preparation steps:
[0010] (1) Take the whole amount of Lampleaf, Dendrobium and Morus alba, decoct them twice in water, and combine the filtrates;
[0011] (2) concentrating the filtrate into a thick extract;
[0012] (3) Take all the Poria cocos, grind them, mix them with the thick extract, add 50-80 g of sucrose, mix them and make granules, add 1 g of magnesium stearate, mix them and press them to make sugar coating, so as to obtain the traditional Chinese medicine composition.
[0013] The beneficial effects of this application are:
[0014] 1. The Chinese medicine composition for treating viral pneumonia in the present application has effective ingredients in its formula only from four raw materials: leaves of Alpinia dahurica, dendrobium, white mulberry bark and Poria cocos, which greatly simplifies the formula. The above four raw materials are widely available and easy to obtain. In addition, when preparing the Chinese medicine composition for treating viral pneumonia based on the above four raw materials, the preparation method is also relatively simple, so the quality of the entire pharmaceutical process is easier to control.
[0015] 2. The Chinese medicine composition for treating viral pneumonia in the present application was used to perform an anti-viral pneumonia zebrafish test, which showed that it has anti-viral pneumonia efficacy. DETAILED DESCRIPTION
[0016] The embodiments of the present application will be described in more detail below with reference to examples. Although the embodiments of the present application are shown in the examples, it should be understood that the present application can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to make the present application more thorough and complete, and the scope of the present application can be fully communicated to those skilled in the art.
[0017] Example 1
[0018] A traditional Chinese medicine composition for treating viral pneumonia, wherein the effective ingredients are prepared from the following raw medicinal materials in the following weight ratio: 1000 g of calendula leaves, 500 g of dendrobium, 300 g of mulberry bark, and 200 g of tuckahoe.
[0019] Furthermore, the Chinese medicine composition is prepared according to the following preparation steps:
[0020] (1) Take the whole amount of Lampleaf, Dendrobium and Morus alba, decoct them twice in water, and combine the filtrates;
[0021] (2) concentrating the filtrate into a thick extract;
[0022] (3) Take all the Poria cocos, grind them, mix them with the thick extract, add 50-80 g of sucrose, mix them and make granules, add 1 g of magnesium stearate, mix them and press them to make sugar coating, so as to obtain the traditional Chinese medicine composition.
[0023] In order to verify the efficacy of the prepared Chinese medicine composition, an antiviral pneumonia zebrafish experiment was conducted.
[0024] The specific experiments are as follows:
[0025] Experimental sample configuration information
[0026] In the Chinese medicine composition prepared in Example 1, the solvent is ultrapure water;
[0027] Positive control: Indomethacin, white powder, batch number D1929098, Shanghai Aladdin Biochemical Technology Co., Ltd., solvent is DMSO.
[0028] Experimental animals
[0029] Zebrafish were raised in fish farming water at 28°C (water quality: 200 mg of instant sea salt was added to every 1 L of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / L CaCO3).
[0030] Experimental methods
[0031] 1. Maximum detectable concentration (MTC) determination
[0032] Transgenic neutrophil green fluorescent MPX strain zebrafish at 5 days post-fertilization (dpf) were randomly selected in 6-well plates, and 30 zebrafish were treated in each well (experimental group). The samples were dissolved in water (concentrations are shown in Table 1-1), and a normal control group and a model control group were set up at the same time, with a capacity of 3 mL per well. Except for the normal control group, poly(I:C) was injected into the swim bladder of the other experimental groups to establish a zebrafish viral pneumonia model. After treatment at 28°C for 3 hours, the MTC of the samples on the model zebrafish was determined.
[0033] Table 1-1 Sample antiviral pneumonia efficacy concentration exploration test results (n = 30)
[0034]
[0035] 2. Evaluation of efficacy against viral pneumonia
[0036] 5dpf transgenic neutrophil green fluorescence MPX strain zebrafish were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well (experimental group). The samples were given water-soluble (concentrations are shown in Table 1-2), the positive control indomethacin concentration was 21.5μg / mL, and the normal control group and model control group were set up at the same time, with a capacity of 3mL per well. Except for the normal control group, the rest of the experimental groups were injected with poly(I:C) into the swim bladder to establish a zebrafish viral pneumonia model. After 3h of treatment at 28℃, 10 zebrafish were randomly selected from each experimental group and photographed under a fluorescence microscope. The data were analyzed and collected using NIS-Elements D 3.20 advanced image processing software, and the number of neutrophils in the swim bladder was analyzed. The statistical analysis results of this indicator were used to evaluate the anti-viral pneumonia efficacy of the sample. The statistical analysis results are expressed as mean±SE. SPSS26.0 software was used for statistical analysis, and p<0.05 indicated that the difference was statistically significant.
[0037] Table 1-2. Sample antiviral pneumonia efficacy evaluation test results (n = 10)
[0038]
[0039] Studies have shown that Poly(I:C) can be used to simulate novel coronavirus infection to study the antiviral efficacy of samples, thereby providing a scientific basis for the treatment of novel coronavirus. Under the experimental conditions, the experimental results show that the traditional Chinese medicine composition prepared in Example 1 has an antiviral pneumonia effect, and it is speculated that it may have an anti-novel coronavirus-induced pneumonia effect.
[0040] Example 2
[0041] The antitussive, expectorant and antiasthmatic effects of the traditional Chinese medicine composition obtained in Example 1 were investigated.
[0042] The phenol red method was used to establish a mouse expectorant model, an ammonia-induced mouse cough model, and a guinea pig asthma model was established by spraying a mixture of acetylcholine chloride and histamine phosphate. Since the pharmacodynamics of this study mainly conducted a preliminary investigation on symptoms such as cough and sputum, the positive control selected the Western medicine pentoxyverine ammonium chloride and the Chinese medicine snake gall, Fritillaria thunbergii and loquat paste, which are suitable for symptoms such as cough and sputum caused by acute and chronic bronchitis. The Dengtai leaf granules were used as the reference drug. The results showed that the Chinese medicine composition obtained in Example 1 had significant expectorant and antitussive effects, and showed a certain antiasthmatic effect; the high-dose group was equivalent to or better than the positive drug, and better than the reference drug. The specific results are as follows:
[0043] (1) Expectorant effect: After administration of the positive drug pentoxyverine ammonium chloride (0.2 g / kg) and the snake gall bladder loquat paste (11 g / kg), the mouse tracheal secretion was significantly promoted; after administration of the reference drug dangtaiye granules (5 g / kg), the mouse tracheal phenol red secretion was significantly increased; after administration of the test substance Chinese medicine composition at 4, 2 and 1 g / kg, the mouse tracheal phenol red secretion was significantly increased, and showed a good dose-dependency. It is worth noting that the high dose of the Chinese medicine composition has the same expectorant effect as the positive drug pentoxyverine ammonium chloride and the snake gall bladder loquat paste, and is better than the reference drug dangtaiye granules.
[0044] (2) Antitussive effect: After administration of the positive drug pentoxyverine ammonium chloride (0.2 g / kg) and snake gall chuanbei loquat paste (11 g / kg), the cough latency of mice was significantly prolonged and the total number of coughs was significantly reduced; after administration of the reference drug dung beetle leaf granules (5 g / kg), the cough latency of mice was significantly prolonged and the total number of coughs was significantly reduced; after administration of the test substance Chinese medicine composition at high doses (4 g / kg) and medium doses (2 g / kg), the cough latency of mice was significantly prolonged and the total number of coughs was significantly reduced. The high dose of the Chinese medicine composition was comparable to the positive drug pentoxyverine ammonium chloride in prolonging the cough latency and was superior to snake gall chuanbei loquat paste, while it was superior to the positive drug pentoxyverine ammonium chloride in reducing the total number of coughs, and its effect was comparable to that of snake gall chuanbei loquat paste. It is worth noting that the high dose of the Chinese medicine composition was superior to the reference drug dung beetle leaf granules in prolonging the cough latency and reducing the total number of coughs.
[0045] (3) Antiasthmatic effect: Administration of the positive drug pentoxyverine ammonium chloride (0.1 g / kg) can prolong the asthma incubation period of guinea pigs, while administration of the positive drug snake gall bladder, Chuanbei loquat paste (5.5 g / kg) has no effect on prolonging the asthma incubation period of guinea pigs; after administration of the reference drug Lampsariae chinensis leaf granules (2.5 g / kg), the asthma incubation period of guinea pigs did not change; while administration of the test substance Chinese medicine composition at a high dose (2 g / kg) can prolong the asthma incubation period of guinea pigs, and is equivalent to that of the positive drug pentoxyverine ammonium chloride.
[0046] The embodiments of the present application have been described above, and the above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments. Many modifications and changes will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments. The selection of terms used herein is intended to best explain the principles of the embodiments, practical applications, or improvements to the technology in the market, or to enable other persons of ordinary skill in the art to understand the embodiments disclosed herein.
Claims
1. A Chinese medicine composition for treating viral pneumonia, characterized in that: The effective ingredients of the traditional Chinese medicine composition are prepared from the following raw medicinal materials in the following weight ratio: 1000g of Lamppost leaf, 500g of Dendrobium officinale, 300g of Morus alba bark and 200g of Poria cocos.
2. A Chinese medicine composition for treating viral pneumonia according to claim 1, characterized in that: Pharmaceutically acceptable excipients are also included.
3. A method for preparing a Chinese medicine composition for treating viral pneumonia according to claim 2, characterized in that: Prepare according to the following preparation steps: (1) Take the whole amount of Lampleaf, Dendrobium and Morus alba, decoct them twice in water, and combine the filtrates; (2) concentrating the filtrate into a thick extract; (3) Take all the Poria cocos, grind them, mix them with the thick extract, add 50-80 g of sucrose, mix them and make granules, add 1 g of magnesium stearate, mix them and press them to make sugar coating, so as to obtain the traditional Chinese medicine composition.