Automatic control feeding system for polypeptide production

Abstract

The invention relates to the technical field of polypeptide production, and discloses an automatic control feeding system for polypeptide production, which comprises a raw material management module, a mechanical arm feeding module, an activation reaction module, a data recording and monitoring module and a result analysis module. The packaging bag made of polyvinyl alcohol is directly dissolved in the organic solvent in the activation tank, the air quality, including the concentration # imgabs0 # of dust particles, in the production environment does not need to be independently disassembled and monitored in real time through a data recording and monitoring module, and if the concentration of the dust particles in the air exceeds the threshold concentration # imgabs1 # of safe operation, the packaging bag is directly dissolved in the organic solvent in the activation tank. When the amino acid packaging bag is opened, the system gives an alarm to prompt an operator to take measures, the system evaluates whether the concentration of dust in the current air affects the human body or not by calculating the Floating Index of the amino acid powder, and the beneficial effect that the human body is not affected when the amino acid packaging bag is opened is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of polypeptide production, in particular to an automatic control feeding system for polypeptide production. Background Art

[0002] The main raw material of polypeptides is protected amino acids. During production, the protected amino acids react in an organic solution to eventually synthesize polypeptides. When feeding the polypeptides, the packaging bags of the protected amino acids need to be opened manually and then put into the activation tank of the polypeptide synthesizer.

[0003] In the operation of unpacking bags with the existing technology, dust can cause harm to the human body. Even if workers wear protective clothing, dust will still adhere to the protective clothing and be inhaled when workers change their work clothes. At the same time, according to the existing factory GMP management situation, GB50073-2001 or ISO14644-1 stipulates that the operation during the period of disassembling the amino acid packaging bags and putting them into the activation tank will affect the cleanliness of the clean room. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides an automatic controlled feeding system for polypeptide production, which has the advantages of not causing any harm to the human body when opening the amino acid packaging bag, and not floating out and affecting the clean room after the amino acid packaging bag is opened, thereby solving the problems of the above-mentioned technology.

[0005] To achieve the above object, the present invention provides the following technical solutions: an automatic control feeding system for polypeptide production, the feeding system comprising: a raw material management module, a robotic arm feeding module, an activation reaction module, a data recording and monitoring module and a result analysis module; The raw material management module is used to store and manage packaging bags, and the packaging bags are made of polyvinyl alcohol; The robotic arm feeding module is used to automatically grab the amino acid packaging bag and put the entire bag into the activation tank; The activation reaction module performs a polypeptide synthesis reaction; The data recording and monitoring module is used to record relevant data, including weighing time, feeding time, synthesis time, environmental cleanliness, TOC and dust data, and calculate whether the amino acid powder has floating phenomenon; The result analysis module is used to analyze the purity and yield of the final product.

[0006] Preferably, the raw material management module includes inventory turnover rate calculation: ; in: The inventory turnover rate is an indicator to measure the efficiency of an enterprise's inventory use; Cost of sales refers to the cost of goods sold during a specific period of time; Average inventory refers to the average level of inventory during a specific period of time.

[0007] The raw material management module also includes average inventory calculation: ; in, Beginning inventory refers to the inventory at the beginning of the time period; Ending inventory refers to the inventory at the end of the time period; is the average inventory.

[0008] Preferably, the raw material management module further includes inventory demand fluctuation, and the inventory demand fluctuation is calculated as: ; in: Safety stock represents the amount of extra inventory kept to cope with fluctuations in demand; is the service level coefficient, indicating the probability of meeting demand without out-of-stock, usually obtained from the standard normal distribution table; The standard deviation of demand measures the volatility of demand data and improves inventory security; is the replenishment cycle, which means the time required from order placement to receipt; Representing the square root of the replenishment cycle, it helps adjust the impact of demand fluctuations on safety stock.

[0009] Preferably, the detailed grabbing steps of the robotic arm feeding module are: S1.1, get instructions: the robot arm receives feeding instructions from the data recording module, the instructions include feeding type, quantity and time; S1.2, sensor feedback: the sensor confirms the number and location of the amino acid packaging bags in the raw material management module, providing necessary information for subsequent operations; S1.3, grabbing action: the robot arm locates the packaging bag, starts the grabbing device, and grabs the amino acid packaging bag with the suction cup. During this process, the grabbing force and grabbing success rate are monitored in real time; S1.4, Move to the activation tank: The robotic arm moves the grabbed packaging bag to the delivery port of the activation tank, releases the grabbing device, and ensures that the packaging bag is correctly delivered to the activation tank; S1.5, Return to initial state: After completing the feeding, the robot arm returns to the initial position and prepares to process the next order.

[0010] Preferably, the specific steps of the activation reaction module are: S2.1, mixing raw materials: adding amino acids and activation reagents into the reactor and mixing them thoroughly with a mechanical stirrer; S2.2, monitoring reaction conditions: using sensors to monitor the temperature and pressure of the reactor in real time to ensure that the reaction conditions meet the set parameters; S2.3, reaction time control: start timing, and control the reaction time to 1 hour to 4 hours according to the desired synthesis type, depending on the type of amino acid and the length of the polypeptide chain; S2.4, Reaction monitoring: Use spectroscopy technology: UV-Vis spectroscopy to monitor the progress of the reaction, and confirm the progress of the reaction based on the characteristic absorption peaks of the reaction intermediates or products; S2.5. Sample collection: Regular sampling is performed to analyze the quality and purity of the reaction intermediates and final products using chromatographic techniques: liquid chromatography (HPLC).

[0011] Preferably, the amino acid powder floating calculation in the data recording and detection module is: ; in: To calculate the index of amino acid powder floating phenomenon; is the concentration of dust particles in the air, in mg / m³; The threshold concentration for safe operation, in mg / m³, is calculated by comparing the concentration of dust particles in the air. Threshold concentration for safe operation The algorithm provides a percentage value indicating the current dust concentration relative to the safe level. If this index exceeds 100%, it indicates that the amino acid powder has begun to float.

[0012] Preferably, the amino acid powder floating calculation in the data recording and detection module is: ; in: It is the concentration of dust particles in the air, measured in milligrams per cubic meter (mg / m³). This value is measured in real time through an online monitoring system and reflects the cleanliness of the production environment. By defining the actual concentration of dust particles in the air, measured in milligrams per cubic meter (mg / m³), the dust concentration in the air is monitored to evaluate the cleanliness and safety of the production environment.

[0013] Preferably, the amino acid powder floating calculation in the data recording and detection module is: ; in: The threshold concentration for safe operation, expressed in milligrams per cubic meter (mg / m³), is determined based on industry standards or experience and indicates an acceptable dust concentration level.

[0014] Preferably, the yield in the result analysis module is calculated as: ; in: The actual mass of product obtained, in grams; is the theoretically obtained product mass.

[0015] Preferably, the estimation calculation in the result analysis module is: ; in: is the mass of the final product obtained after purification, g; is the mass of all products obtained after the reaction, g.

[0016] Compared with the prior art, the present invention provides an automatic control feeding system for polypeptide production, which has the following beneficial effects: 1. The present invention uses packaging bags made of polyvinyl alcohol, which are directly dissolved in the organic solvent in the activation tank, and no longer need to be disassembled separately. At the same time, the robotic arm is responsible for automatically grabbing the amino acid packaging bag. The entire process is operated by the robotic arm without direct manual contact. During the grabbing process, the system monitors the grabbing force in real time to ensure that the packaging bag will not be damaged by excessive squeezing, thereby avoiding the leakage of amino acid powder. The system monitors the grabbing success rate in real time to ensure that each grab is successful and reduce accidents caused by improper operation. The data recording and monitoring module monitors the air quality in the production environment in real time, including the concentration of dust particles. If the concentration of dust particles in the air exceeds the threshold concentration for safe operation , the system will sound an alarm and prompt the operator to take measures. By calculating the floating index of the amino acid powder, the system evaluates whether the current dust concentration in the air has an impact on the human body. If the floating index exceeds 100%, the system will automatically take measures, such as increasing ventilation or suspending operation, to ensure the safety of the operating environment, achieving the beneficial effect of opening the amino acid packaging bag without causing any impact on the human body.

[0017] 2. The packaging bag made of polyvinyl alcohol in the present invention has good sealing performance, ensuring that the amino acid powder will not leak during transportation and storage, and the packaging bag can also be dissolved in the organic solvent in the activation tank. By calculating the inventory turnover rate and safety inventory, the utilization efficiency and freshness of the raw materials are ensured, and the risk of packaging bag aging and powder leakage caused by long-term storage is reduced. The robotic arm puts the entire amino acid packaging bag into the activation tank, avoiding the flying of powder during the bag unpacking process, and monitoring the concentration of dust particles in the air. And with the safe threshold concentration By comparison, the floating index is calculated. If the floating index exceeds 100%, it indicates that the amino acid powder may have begun to float. The system will trigger an alarm and take corresponding measures, such as starting the air purification system or stopping the operation to prevent further diffusion of the powder, thereby achieving the beneficial effect of preventing the amino acid powder from floating out and affecting the clean room after the amino acid packaging bag is opened. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the system of the present invention; DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] See also Figure 1 , a polypeptide production automatic control feeding system, the feeding system consists of: raw material management module, robotic arm feeding module, activation reaction module, data recording and monitoring module and result analysis module; The raw material management module is used to store and manage packaging bags, which are made of polyvinyl alcohol (PVA); The robotic arm feeding module is used to automatically grab the amino acid packaging bag and put the whole bag into the activation tank; The activation reaction module performs a peptide synthesis reaction; The data recording and monitoring module is used to record relevant data, including weighing time, feeding time, synthesis time, environmental cleanliness, TOC and dust data, and to calculate whether the amino acid powder has floating phenomenon; The result analysis module is used to analyze the purity and yield of the final product.

[0021] By using packaging bags made of PVA materials, they are directly dissolved in the organic solvent in the activation tank and no longer need to be disassembled separately. The robotic arm feeding module automatically grabs the amino acid packaging bag and puts the whole bag into the activation tank, avoiding the errors and risks caused by manual operation and improving operational efficiency and consistency.

[0022] In the present application, the organic solvent in the activation tank is dimethyl sulfoxide (DMSO).

[0023] Experiment 1: Use different solvents to conduct dissolution tests on the above PVA packaging bags. In six test tubes, add 20 ml of dimethylformamide (DMF), dimethyl sulfoxide (DMSO), dichloromethane (DCM), ethyl acetate (EtOAc), water and N-methylpyrrolidone (NMP) solvents, and then add about 1 gram of PVA packaging bags to each solvent. Observe the dissolution at 1 hour and 2 hours respectively. The dissolution is summarized in Table 1 below: Table 1 As shown in Table 1, the PVA packaging bag was completely dissolved in DMSO, but not dissolved in DMF, DCM, and EtOAc. The PVA packaging bag could be partially dissolved in NMP solvent and water.

[0024] Experiment 2: Add about 1 gram of PVA packaging bag and 5 grams of protected amino acid to the above six test tubes, and add 20 ml of dimethylformamide (DMF), dimethyl sulfoxide (DMSO), dichloromethane (DCM), ethyl acetate (EtOAc), water and N-methylpyrrolidone (NMP) solvents. Observe the dissolution at 1 hour and 2 hours respectively. The dissolution is summarized in Table 2 below: Table 2 As shown in Table 2, the PVA bag completely dissolved in DMSO, but not in DCM and EtOAc. It was mixed in water. In DMF and NMP solvents, the amino acid was soluble but the bag was not. These results show that the solubility of the bag in the studied solvent will also be different after the sample is filled. Experiment 3: Add about 2 grams of PVA packaging bag and 10 grams of protected amino acid to the above six test tubes, and then add 20 ml of dimethylformamide (DMF) and 20 ml of dimethyl sulfoxide (DMSO) solvent. Observe the dissolution at 1 hour and 2 hours respectively. The dissolution is summarized in Table 3 below: Table 3 Experiment 4: Add about 1 gram of PVA packaging bag and 5 grams of protected amino acid to the above six test tubes respectively, add dimethyl sulfoxide (DMSO) solvent first and observe the dissolution after 30 minutes, then add dimethylformamide (DMF) and mix to a total solvent of 20 ml, and observe the dissolution after 1 hour. The solvent ratios of DMSO and DMF are DMF / DMSO=80 / 20, DMF / DMSO=75 / 25, and DMF / DMSO=70 / 30, respectively. The dissolution is summarized in Table 4 below: Table 4 The results showed that PVA packaging bags could not be dissolved in the above proportions of solvents.

[0025] Experiment 5: Add about 1 gram of PVA packaging bag and 1 gram of protected amino acid to the above six test tubes respectively, add dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) mixed solution, the mixing ratio of DMF / DMSO=50 / 50, DMF / DMSO=70 / 30, DMF / DMSO=60 / 40, the initial amount is 20 ml, and then add 10 ml each time, and finally add to 40 ml, record the dissolution time and dissolution state, and summarize them in the following Table 5: Table 5 The results showed that the PVA packaging bag could be dissolved when the proportion of DMSO reached 50%, but was only partially dissolved at other proportions.

[0026] Experiment 6: About 1 gram of PVA packaging bag and 0.4 mmol of protected amino acid were added to the above six test tubes respectively, and a mixed solution of dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) was added, with a mixing ratio of DMF / DMSO = 70 / 30 and DMF / DMSO = 60 / 40. The initial amount was 20 ml, and then 10 ml was added each time until the final amount was 40 ml. The dissolution time and dissolution state were recorded, and the summary is shown in Table 6 below: Table 6 The results showed that after reducing the weight of amino acids, the solvent in the above ratio could dissolve the amino acids but could not dissolve the bag.

[0027] Experiment 7: About 2 mg of PVA packaging bag and 0.4 mmol of protected amino acid were added to the above six test tubes respectively, and a mixed solution of dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) was added, with a mixing ratio of DMF / DMSO = 70 / 30 and DMF / DMSO = 60 / 40. The initial amount was 20 ml, and then 10 ml was added each time until it reached 40 ml. The dissolution time and dissolution state were recorded, and the results are summarized in Table 7 below: Table 7 The results showed that after the bag was reduced to the minimum weight that could package 0.4 mmol of amino acids, both the amino acids and the bag could be dissolved at 40% DMSO, but the bag could not be dissolved when DMSO was reduced to 30%.

[0028] Experiment 8: According to the weighing ratio of the above experiment, scale up to 0.1g bag and 5g amino acid, add dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) mixed solution, the mixing ratio DMF / DMSO=60 / 40, DMF / DMSO=50 / 50, the starting amount is 60ml, then add 10ml each time, and finally add to 100ml, record the dissolution time and dissolution state, and summarize as follows: Table 8 The results showed that after the weight of the bag and amino acids were scaled up, the amino acids and the bag were not completely dissolved at 40% DMSO, and the bag dissolved when DMSO was increased to 50%, but the amino acids were still not completely dissolved, indicating that the bag had a higher solubility at a 50% DMSO ratio.

[0029] Experiment 9: Add about 60 mg of PVA packaging bags to each of the six test tubes, add 10 ml of dimethyl sulfoxide (DMSO), and observe the dissolution status and time. If the bag dissolves, add 5 g of protected amino acids and 30 ml of dimethylformamide (DMF), and observe the dissolution time and dissolution status. The results are summarized as follows: Table 9 The results showed that under the above experimental conditions, both the amino acids and the bags could be completely dissolved.

[0030] Raw material management module: automatically manage raw material inventory, reduce manual intervention, and ensure the accuracy of raw material storage and use.

[0031] Activation reaction module: automatically performs peptide synthesis reactions, accurately controls reaction conditions, and reduces errors introduced by human operation.

[0032] Improve efficiency: Automated operation reduces the time for feeding and reaction, and improves production efficiency.

[0033] Consistency: Automation ensures that the feed amount and reaction conditions are consistent for each batch of production, ensuring the stability of product quality.

[0034] Inventory turnover rate calculation: By calculating inventory turnover rate and safety stock, the efficiency of raw material use can be optimized to avoid raw material backlog or shortage.

[0035] High quality packaging bag material: The packaging bag is made of polyethylene glycol dimethacrylate (PEG2000DMA), which has good sealing performance and reduces the leakage of raw materials.

[0036] Production costs are reduced by optimizing inventory management and avoiding raw material waste.

[0037] Reduce raw material contamination: High-quality packaging bag materials reduce the risk of raw materials being contaminated during storage and transportation.

[0038] Precise grabbing: The robot arm confirms the position of the packaging bag through sensors and uses suction cups to grab, ensuring the grabbing success rate and feeding accuracy.

[0039] Put the whole bag into the activation tank to avoid powder floating during bag unpacking and reduce the impact on the clean room environment.

[0040] Reduce dust pollution: Putting whole bags into the container avoids the flying of powder and ensures the cleanliness of the clean room environment.

[0041] Improve feeding accuracy: The precise operation of the robotic arm ensures the accuracy of each feeding.

[0042] Real-time monitoring of environmental parameters: recording weighing time, feeding time, synthesis time, environmental cleanliness, TOC and dust data.

[0043] Floating phenomenon calculation: The floating index is used to calculate whether the amino acid powder has floating phenomenon. If it exceeds the safety threshold (100%), the system will take measures.

[0044] Specifically, the raw material management module includes inventory turnover calculation: ; in: The inventory turnover rate is an indicator to measure the efficiency of an enterprise's inventory use; Cost of sales refers to the cost of goods sold during a specific period of time; Average inventory refers to the average level of inventory during a specific period of time.

[0045] The Material Management module also includes average inventory calculations: ; in, Beginning inventory refers to the inventory at the beginning of the time period; Ending inventory refers to the inventory at the end of the time period; is the average inventory.

[0046] Specifically, the raw material management module also includes inventory demand fluctuations, which are calculated as follows: ; in: Safety stock represents the amount of extra inventory kept to cope with fluctuations in demand; is the service level coefficient, indicating the probability of meeting demand without out-of-stock, usually obtained from the standard normal distribution table; The standard deviation of demand measures the volatility of demand data and improves inventory security; is the replenishment cycle, which means the time required from order placement to receipt; Representing the square root of the replenishment cycle, it helps adjust the impact of demand fluctuations on safety stock.

[0047] Specifically, the detailed grasping steps of the robotic arm feeding module are as follows: S1.1, get instructions: the robot arm receives feeding instructions from the data recording module, the instructions include feeding type, quantity and time; S1.2, sensor feedback: the sensor confirms the number and location of the amino acid packaging bags in the raw material management module, providing necessary information for subsequent operations; S1.3, grabbing action: the robot arm locates the packaging bag, starts the grabbing device, and grabs the amino acid packaging bag with the suction cup. During this process, the grabbing force and grabbing success rate are monitored in real time; S1.4, Move to the activation tank: The robotic arm moves the grabbed packaging bag to the delivery port of the activation tank, releases the grabbing device, and ensures that the packaging bag is correctly delivered to the activation tank; S1.5, Return to initial state: After completing the feeding, the robot arm returns to the initial position and prepares to process the next order.

[0048] Specifically, the specific steps of activating the reaction module are: S2.1, mixing raw materials: adding amino acids and activation reagents into the reactor and mixing them thoroughly with a mechanical stirrer; S2.2, monitoring reaction conditions: using sensors to monitor the temperature and pressure of the reactor in real time to ensure that the reaction conditions meet the set parameters; S2.3, reaction time control: start timing, and control the reaction time to 1 hour to 4 hours according to the desired synthesis type, depending on the type of amino acid and the length of the polypeptide chain; S2.4, Reaction monitoring: Use spectroscopy technology: UV-Vis spectroscopy to monitor the progress of the reaction, and confirm the progress of the reaction based on the characteristic absorption peaks of the reaction intermediates or products; S2.5. Sample collection: Regular sampling is performed to analyze the quality and purity of the reaction intermediates and final products using chromatographic techniques: liquid chromatography (HPLC).

[0049] Specifically, the amino acid powder floating calculation in the data recording and detection module is: ; in: To calculate the index of amino acid powder floating phenomenon; is the concentration of dust particles in the air, in mg / m³; The threshold concentration for safe operation, in mg / m³, is calculated by comparing the concentration of dust particles in the air. Threshold concentration for safe operation The algorithm provides a percentage value indicating the current dust concentration relative to the safe level. If this index exceeds 100%, it indicates that the amino acid powder has begun to float.

[0050] Specifically, the amino acid powder floating calculation in the data recording and detection module is: ; in: It is the concentration of dust particles in the air, measured in milligrams per cubic meter (mg / m³). This value is measured in real time through an online monitoring system and reflects the cleanliness of the production environment. By defining the actual concentration of dust particles in the air, measured in milligrams per cubic meter (mg / m³), the dust concentration in the air is monitored to evaluate the cleanliness and safety of the production environment.

[0051] Specifically, the amino acid powder floating calculation in the data recording and detection module is: ; in: The threshold concentration for safe operation, expressed in milligrams per cubic meter (mg / m³), is determined based on industry standards or experience and indicates an acceptable dust concentration level.

[0052] Specifically, the yield calculation in the result analysis module is: ; in: The actual mass of product obtained, in grams; is the theoretically obtained product mass.

[0053] Specifically, the estimation calculation in the result analysis module is: ; in: is the mass of the final product obtained after purification, g; is the mass of all products obtained after the reaction, g.

[0054] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A polypeptide production automatic control feeding system, characterized in that: The feeding system is composed of: a raw material management module, a robotic arm feeding module, an activation reaction module, a data recording and monitoring module and a result analysis module; The raw material management module is used to store and manage packaging bags, and the packaging bags are made of polyvinyl alcohol; The robotic arm feeding module is used to automatically grab the amino acid packaging bag and put the entire bag into the activation tank; The activation reaction module performs a polypeptide synthesis reaction; The data recording and monitoring module is used to record relevant data, including weighing time, feeding time, synthesis time, environmental cleanliness, TOC and dust data, and calculate whether the amino acid powder has floating phenomenon; The result analysis module is used to analyze the purity and yield of the final product.

2. The polypeptide production automatic control feeding system according to claim 1, characterized in that: The raw material management module is also used to calculate the inventory turnover rate, and the calculation formula is as follows: ； in: The inventory turnover rate is an indicator to measure the efficiency of an enterprise's inventory use; Cost of sales refers to the cost of goods sold during a specific period of time; The average inventory refers to the average inventory level within a specific time period; wherein the raw material management module is used to calculate the average inventory, and the calculation formula is as follows: ； in, Beginning inventory refers to the inventory at the beginning of the time period; Ending inventory refers to the inventory at the end of the time period; is the average inventory.

3. The polypeptide production automatic control feeding system according to claim 2, characterized in that: The raw material management module is also used to calculate inventory demand fluctuations, and the calculation formula is as follows: ； in: Safety stock represents the amount of extra inventory kept to cope with fluctuations in demand; is the service level coefficient, indicating the probability of meeting demand without out-of-stock, usually obtained from the standard normal distribution table; The standard deviation of demand measures the volatility of demand data and improves inventory security; is the replenishment cycle, which means the time from order placement to receipt; Representing the square root of the replenishment cycle, it helps adjust the impact of demand fluctuations on safety stock.

4. The polypeptide production automatic control feeding system according to claim 1, characterized in that: The detailed grasping steps of the robotic arm feeding module are as follows: S1.1, get instructions: the robot arm receives feeding instructions from the data recording module, the instructions include feeding type, quantity and time; S1.2, sensor feedback: the sensor confirms the number and location of the amino acid packaging bags in the raw material management module, providing necessary information for subsequent operations; S1.3, grabbing action: the robot arm locates the packaging bag, starts the grabbing device, and grabs the amino acid packaging bag with the suction cup. During this process, the grabbing force and grabbing success rate are monitored in real time; S1.4, Move to the activation tank: The robotic arm moves the grabbed packaging bag to the delivery port of the activation tank, releases the grabbing device, and ensures that the packaging bag is correctly delivered to the activation tank; S1.5, Return to initial state: After completing the feeding, the robot arm returns to the initial position and prepares to process the next order.

5. The polypeptide production automatic control feeding system according to claim 1, characterized in that: The specific steps of the activation reaction module are: S2.1, mixing raw materials: adding amino acids and activation reagents into the reactor and mixing them thoroughly with a mechanical stirrer; S2.2, monitoring reaction conditions: using sensors to monitor the temperature and pressure of the reactor in real time to ensure that the reaction conditions meet the set parameters; S2.3, reaction time control: start timing, and control the reaction time to 1 hour to 4 hours according to the desired synthesis type, depending on the type of amino acid and the length of the polypeptide chain; S2.4, Reaction monitoring: Use spectroscopy technology: UV-Vis spectroscopy to monitor the progress of the reaction, and confirm the progress of the reaction based on the characteristic absorption peaks of the reaction intermediates or products; S2.

5. Sample collection: Regular sampling is performed to analyze the quality and purity of the reaction intermediates and final products using chromatographic techniques: liquid chromatography (HPLC).

6. The polypeptide production automatic control feeding system according to claim 1, characterized in that: The amino acid powder floating calculation in the data recording and detection module is: ； in: To calculate the index of amino acid powder floating phenomenon; is the concentration of dust particles in the air, in mg / m³; The threshold concentration for safe operation, in mg / m³, is calculated by comparing the concentration of dust particles in the air. Threshold concentration for safe operation The algorithm provides a percentage value indicating the current dust concentration relative to the safe level. If this index exceeds 100%, it indicates that the amino acid powder has begun to float.

7. The polypeptide production automatic control feeding system according to claim 1, characterized in that: The amino acid powder floating calculation in the data recording and detection module is: ； in: It is the concentration of dust particles in the air, measured in milligrams per cubic meter (mg / m³). This value is measured in real time through an online monitoring system and reflects the cleanliness of the production environment. By defining the actual concentration of dust particles in the air, measured in milligrams per cubic meter (mg / m³), the dust concentration in the air is monitored to evaluate the cleanliness and safety of the production environment.

8. The polypeptide production automatic control feeding system according to claim 1, characterized in that: The amino acid powder floating calculation in the data recording and detection module is: ； in: The threshold concentration for safe operation, expressed in milligrams per cubic meter (mg / m³), is determined based on industry standards or experience and indicates an acceptable dust concentration level.

9. The polypeptide production automatic control feeding system according to claim 1, characterized in that: The yield in the result analysis module is calculated as: ； in: The actual mass of product obtained, in grams; is the theoretically obtained product mass.

10. The polypeptide production automatic control feeding system according to claim 1, characterized in that: The scale calculation formula in the result analysis module is: ； in: is the mass of the final product obtained after purification, g; is the mass of all products obtained after the reaction, g.

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