Backup liquid cooling table and liquid transfer equipment

Abstract

The invention provides a standby liquid cooling table and liquid transferring equipment, and relates to the field of medical equipment. The standby liquid cooling table comprises a bearing part and a cooling table assembly, the bearing part is used for placing a plurality of culture dishes, and the bearing part is of a heat conduction structure with heat conduction capability; the cold table assembly is provided with a containing table top which is detachably connected with the bearing piece. The cooling table assembly can adjust the temperature of the bearing piece located on the object placing table top. According to the liquid preparation cooling table provided by the invention, the culture solution and the culture oil can be automatically dripped into a plurality of culture dishes under a set temperature condition, and the stability of the osmotic pressure of the culture solution is effectively maintained in the liquid dripping process.

Description

Technical Field

[0001] The invention relates to the field of medical equipment, and in particular to a liquid preparation cold stage and liquid transfer equipment. Background Art

[0002] In the early preparation of assisted reproductive technology in reproductive medicine laboratories, manual pipetting and oil dripping are required. Most of the time, the culture dishes are pipetted and dripped one by one and then immediately transferred to the incubator. Obviously, this method is inefficient, time-consuming and labor-intensive, and is not suitable for pipetting and dripping a large number of culture dishes. In addition, during the preparation process, the temperature of the culture dishes is not effectively controlled, resulting in the risk of changes in the osmotic pressure of the culture solution, which in turn poses a potential risk to the later embryo culture. Summary of the invention

[0003] In view of this, the purpose of the present invention is to overcome the deficiencies in the prior art and to provide a liquid preparation cold stage and a pipetting device that can automatically drip culture solution and culture oil into multiple culture dishes under set temperature conditions and effectively maintain the stability of the osmotic pressure of the culture solution during the dripping process.

[0004] The present invention provides the following technical solutions:

[0005] The embodiment of the present application provides a liquid standby cooling stage, the liquid standby cooling stage comprising:

[0006] A carrier, the carrier is used to place a plurality of culture dishes, and the carrier is a heat-conducting structure with heat-conducting capability;

[0007] A cold stage assembly, wherein the cold stage assembly has a storage table, and the storage table can be detachably connected to the carrier; wherein the cold stage assembly can adjust the temperature of the carrier located on the storage table.

[0008] In some embodiments of the first aspect, the cold stage assembly comprises:

[0009] A temperature regulating member is detachably connected to the supporting member, and the temperature regulating member can regulate the temperature of the supporting member.

[0010] In some embodiments of the first aspect, the temperature regulating member comprises:

[0011] A semiconductor refrigerator, wherein the semiconductor refrigerator has a cooling end, the cooling end abuts against the supporting member, and the cooling end is used to place the supporting member.

[0012] In some embodiments of the first aspect, the carrier comprises:

[0013] The dish tray has a plurality of limiting grooves, and the limiting grooves can accommodate the culture dish.

[0014] In some embodiments of the first aspect, the cold stage assembly further comprises:

[0015] A refrigerating unit, wherein the refrigerating unit has an inner cavity, wherein the inner cavity has a loading position and a buffer position, wherein the loading position is used to store opened solution bottles, and the buffer position is used to store unopened solution bottles; wherein the refrigerating unit has a liquid collection port, wherein the liquid collection port is connected to the solution bottle located at the loading position.

[0016] In some embodiments of the first aspect, the refrigeration unit comprises:

[0017] A box body, the box body having an opening; wherein the refrigeration end portion is located in the box body, and the liquid taking port is arranged in the box body, so that the loading position and the buffer position are formed in the box body;

[0018] A storage rack, the storage rack is slidably connected to the box body, the storage rack can enter and exit the box body from the opening, and the loading position and the buffer position are both arranged on the moving track of the storage rack; wherein the storage rack is used to place the unopened solution bottles and the opened solution bottles.

[0019] In some embodiments of the first aspect, the refrigeration unit further comprises:

[0020] An identification unit, the identification unit is installed on the box body, the identification unit is electrically connected to the pipetting assembly, and the identification unit can obtain the bottle body information of the solution bottle located at the loading position, and control the pipetting assembly to send a control instruction according to the bottle body information of the solution bottle;

[0021] The bottle body information includes one or more of the following: liquid level information in the solution bottle, position information of the solution bottle, bottle mouth sealing status information of the solution bottle, and label information of the solution bottle.

[0022] In a second aspect, the present application further provides a liquid transfer device, the liquid transfer device comprising:

[0023] N pipetting components, each of which can drip a corresponding target liquid medium into a culture dish located in a preset area; wherein a plurality of culture dishes can be placed in the preset area, and N is a positive integer, and N≥2;

[0024] A cold stage assembly, wherein the cold stage assembly is a liquid preparation cold stage as described in any one of the above embodiments, and the culture dish located on the carrier is located in the preset area.

[0025] In some embodiments of the second aspect, the pipetting assembly comprises:

[0026] A pipetting element, which is capable of sucking and discharging the target liquid medium;

[0027] A first driving member, the first driving member is connected to the liquid transfer member, and the first driving member can drive the liquid transfer member to move in a first direction;

[0028] A second driving member, the second driving member is connected to the first driving member, and the second driving member is used to drive the first driving member to move in a first reference plane; wherein the first direction and the first reference plane are arranged perpendicularly, and the first reference plane and the storage table are parallel.

[0029] In some embodiments of the second aspect, the pipetting device further includes M cover-opening components, at least one of the N pipetting components is connected to two cover-opening components, M is a positive integer, and M≥2; wherein the cover-opening component includes:

[0030] A cover opening member, wherein the cover opening member is capable of grabbing the lid of the culture dish;

[0031] A third driving member, the third driving member is connected to the cover opening member, and the third driving member is connected to the second driving member, the second driving member is used to drive the second driving member to move within the first reference plane, and the third driving member is used to drive the cover opening member to move in the first direction.

[0032] In some embodiments of the second aspect, the liquid preparation cold stage further includes an identification component, wherein the identification component is electrically connected to the pipetting component and the lid opening component, respectively, and the second driving member is connected to the identification component, wherein the second driving member is used to drive the identification component to move within the first reference plane, and the identification component is capable of acquiring the current dish body information of the culture dish, and controlling the pipetting component to switch between performing a dripping action and controlling the lid opening component to perform an action of taking and placing the dish cover according to the dish body information; wherein the dish body information includes first information of the dish cover of the culture dish and second information of the dish bottom of the culture dish.

[0033] The embodiments of the present invention have the following advantages:

[0034] By adopting the liquid preparation cold stage provided by the present invention, a plurality of culture dishes are carried by the cold stage assembly, and the temperature of the culture dishes is adjusted, so that the culture dishes can be kept in an environment of 2-8°C, and the influence of temperature change on the culture success rate can be reduced. The culture solution and culture oil can be automatically dripped into the plurality of culture dishes under the set temperature condition, and the osmotic pressure of the culture solution can be effectively maintained during the dripping process, so as to serve as a basis for a pair of pipetting assemblies to batch drip the culture solution and culture oil into the plurality of culture dishes, thereby reducing the environmental requirements for the culture dishes, and all the culture dishes on the storage table of the cold stage assembly can be uniformly transferred to the incubator after the dripping is completed, so the working efficiency is high, and batch pipetting and oil dripping can be performed on a large number of culture dishes.

[0035] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and understandable, preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0037] Figure 1 A structural schematic diagram of a liquid preparation component provided by an embodiment of the present invention from one perspective is shown;

[0038] Figure 2 A schematic structural diagram showing another perspective of a liquid preparation component provided by an embodiment of the present invention;

[0039] Figure 3 A structural schematic diagram of a second liquid transfer assembly provided by an embodiment of the present invention from one viewing angle is shown;

[0040] Figure 4 A schematic structural diagram of a second liquid transfer assembly provided by an embodiment of the present invention from another perspective is shown;

[0041] Figure 5 A schematic structural diagram of a temperature regulating component of a cold stage assembly provided by an embodiment of the present invention is shown;

[0042] Figure 6 A schematic structural diagram of a bearing member of a cold stage assembly provided by an embodiment of the present invention is shown;

[0043] Figure 7 A schematic structural diagram of a refrigeration component of a cold stage assembly provided by an embodiment of the present invention is shown.

[0044] Description of main component symbols:

[0045] 100-carrying part; 110-handle; 200-refrigerating part; 210-liquid extraction port; 220-identification part; 230-box; 240-loading position; 250-solution bottle; 260-cache position; 270-shelf; 271-end cover; 300-culture dish; 400-temperature regulating part; 410-refrigeration end; 420-semiconductor refrigerator; 430-radiator; 500-second pipetting component; 510-second driving part; 520-first driving part; 530-pipetting part; 600-first pipetting component; 700-identification component; 800-opening component; 810-third driving part; 820-opening part; 900-first rack. DETAILED DESCRIPTION

[0046] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0047] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. In contrast, when an element is referred to as being "directly on" another element, there is no intermediate element. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0048] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0049] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0050] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of the template are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more of the related listed items.

[0051] In assisted reproductive technology, sperm, eggs, and embryos need to be provided with the nutrients required for in vitro development to support the embryo's development process from the fertilized egg under strictly controlled environmental conditions until it reaches the predetermined developmental stage or is transplanted back into the mother.

[0052] However, in the process of pipetting and dripping oil, most of the culture dishes 300 are manually pipetted and dripped oil one by one and then immediately transferred to the incubator. Obviously, this method is inefficient and time-consuming. In addition, during the preparation of the dish, the temperature of the culture dish 300 is not effectively controlled, resulting in the risk of changes in the osmotic pressure of the culture solution, which in turn poses a potential risk to the later embryo culture.

[0053] like Figure 1 and Figure 2 As shown, in order to solve the above technical problems, the embodiment of the present application provides a liquid preparation cold stage including a carrier 100 and a cold stage assembly, wherein the carrier 100 is used to place a plurality of culture dishes 300, and the carrier 100 is a heat-conducting structure with heat-conducting capability; the cold stage assembly has a storage table, and the storage table can be detachably connected to the carrier 100; wherein the cold stage assembly can adjust the temperature of the carrier 100 located on the storage table.

[0054] The present embodiment is to drip culture fluid and culture oil. Of course, in other embodiments, it can also be used to drip multiple culture oils and multiple culture fluids, which are not specifically limited here.

[0055] In this embodiment, the storage table of the cold stage assembly is placed in a preset area, so that the carrier 100 loaded with multiple culture dishes 300 can be placed on the storage table. For example, the storage table is set as a horizontal plane, and the culture dishes 300 in the carrier 100 are placed horizontally. It should be noted that the temperature of the culture dishes 300 can be adjusted by the cold stage assembly, so that the culture dishes 300 are basically maintained at an environment of 2-8°C during the batch instillation process.

[0056] Exemplarily, the cold table assembly is a compressor and an evaporating dish, and the evaporating dish is arranged on the placement table to realize refrigeration of the placement table, and the temperature is lowered by heat exchange between the placement table and the culture dish 300. The placement table forms a storage table. Alternatively, in other embodiments, a heat exchange channel is arranged in the heat exchange block, and cooling water is passed through the heat exchange channel, and the heat exchange block forms a storage table, thereby being able to cool the culture dish 300.

[0057] In this embodiment, the culture dish 300 takes a circular culture dish 300 as an example. Of course, in other implementations, culture dishes 300 of other shapes may also be used, such as square culture dishes 300, rectangular culture dishes 300, U-shaped culture dishes 300, V-shaped culture dishes 300, etc., which are not specifically limited here.

[0058] When the culture dish 300 is placed on the carrier 100, if the temperature regulating member 400 increases the temperature of the carrier 100, the temperature of the culture dish 300 will also increase; if the temperature regulating member 400 lowers the temperature of the carrier 100, the temperature of the culture dish 300 will also lower, thereby achieving the control of the temperature of the culture dish 300 during the dripping liquid and oil process.

[0059] It should be noted that the carrier 100 can place multiple culture dishes 300 at the same time. For example, the carrier 100 places 2 culture dishes 300; of course, in other embodiments, the number of culture dishes 300 that can be placed on the carrier 100 is 3, 4, 5, 6, 7, 8, etc., which are not specifically limited here. The number of culture dishes 300 actually placed is set according to the use of the carrying area of ​​the carrier 100 to sum. That is to say, when the number of culture dishes 300 actually required to be set is 1, only one culture dish 300 can be placed on the carrier 100; when the number of culture dishes 300 actually required to be set is 16, but the carrier 100 can only place 8 culture dishes 300 when fully loaded, then the carrier 100 can only place 8 culture dishes 300.

[0060] Among them, since the culture dish 300 is placed on the carrier 100, there is a contact portion between the two, and the contact portion can conduct heat. For those skilled in the art, the contact area between the culture dish 300 and the carrier 100 can be adjusted according to actual conditions, so as to achieve the purpose of adjusting the heat conduction efficiency, and the temperature control is also more accurate. For example, the entire bottom of the culture dish 300 is set to abut against the carrier 100. Of course, when the heat conduction efficiency meets the requirements, the bottom of the culture dish 300 can also be set to partially abut against the carrier 100.

[0061] Similarly, the thermal conductivity of the carrier 100 will also affect the efficiency of heat conduction. For example, if the carrier 100 is made of a material with poor thermal conductivity, the thermal conduction efficiency of the carrier 100 and the culture dish 300 will also be low; if the carrier 100 is made of a material with good thermal conductivity, the thermal conduction efficiency of the carrier 100 and the culture dish 300 will also be high. Based on this, the carrier 100 can be made of a metal material with good thermal conductivity, such as silver (Ag), copper (Cu), aluminum (Al), etc. Of course, in other embodiments, it can also be made of non-metallic materials with good thermal conductivity, such as graphite, carbon nanotubes, carbon fibers, silicon, boron nitride, etc., which are not specifically limited here.

[0062] By using the liquid preparation cold stage provided by the present invention, multiple culture dishes 300 are carried by the cold stage assembly, and the temperature of the culture dishes 300 is adjusted, so that the culture dishes 300 can be kept in an environment of 2-8°C, and the influence of temperature change on the culture success rate can be reduced. The culture solution and culture oil can be automatically dripped into the multiple culture dishes 300 under the set temperature condition, and the osmotic pressure of the culture solution can be effectively maintained during the dripping process, so as to serve as a basis for a pair of pipetting assemblies to batch drip the culture solution and culture oil into the multiple culture dishes 300, thereby reducing the environmental requirements for the culture dishes 300. After the dripping is completed, all the culture dishes 300 on the storage table of the cold stage assembly can be uniformly transferred to the incubator, with high working efficiency, and batch pipetting and dripping of oil can be performed on a large number of culture dishes 300.

[0063] In some embodiments, the cold stage assembly includes a temperature regulating member 400 . The temperature regulating member 400 and the carrier 100 are detachably connected. The temperature regulating member 400 can regulate the temperature of the carrier 100 .

[0064] In this embodiment, the temperature regulating member 400 is used to lower the temperature of the carrier 100 to be lower than the ambient temperature, so that it is at a target temperature of 2-8°C, that is, the culture dish 300 is basically in a temperature environment of 2-8°C.

[0065] Of course, if the required temperature of the culture dish 300 is higher than the ambient temperature, the temperature adjustment member 400 is used to increase the temperature of the carrier 100. Obviously, whether the temperature adjustment member 400 is used for heating or cooling is set according to the actual scene.

[0066] By using the cooperation of the carrier 100 and the temperature regulating member 400, a plurality of culture dishes 300 to be dripped can be placed on the carrier 100, and the temperature of the carrier 100 can be adjusted in real time by the temperature regulating member 400, so that the temperature of the culture dishes 300 located on the carrier 100 can be controlled at intervals so that they are basically in an environment that meets the set temperature requirements, and then after the dripping of batch culture dishes 300 is completed, the culture dishes 300 that have been dripped can be transferred in batches, thereby avoiding frequent opening of the incubator and improving work efficiency. In addition, by controlling the temperature of the culture dishes 300 during the dripping stage, the success rate of the experimental culture can be improved.

[0067] It should be noted that using the carrier 100 to keep the culture dish 300 warm can reduce human error in evaluating the temperature and improve the standardization of the experimental results. For example, the temperature of the liquid can be accurately and efficiently controlled during the dripping and transfer process, reducing the impact of human error in dripping and transfer.

[0068] like Figure 5As shown, in some embodiments, the temperature adjustment element 400 includes a semiconductor refrigerator 420 and a power commutator, the semiconductor refrigerator 420 has a cooling end 410 and a heating end, and the heating end is connected to a heat sink 430; the power commutator and the semiconductor refrigerator 420 are electrically connected, and the power commutator is configured to at least be able to adjust the direction of the current connected to the semiconductor refrigerator 420.

[0069] In this embodiment, by energizing the semiconductor refrigerator 420, the electric energy is used for cooling or heating, thereby adjusting the temperature of the carrier 100 in contact with the cooling end 410 of the semiconductor refrigerator 420 for heat conduction. In order to ensure the heat conduction efficiency between the carrier 100 and the semiconductor refrigerator 420, the carrier 100 and the semiconductor refrigerator 420 can be set to be in surface contact to improve the heat conduction efficiency.

[0070] In addition, since the carrier 100 needs to be frequently taken and placed, in order to reduce the wear on the cooling end 410, a heat conducting plate is provided at the cooling end 410, and the cooling end 410 contacts the carrier 100 through the heat conducting plate. In order to ensure the fit between the cooling end 410 and the heat conducting plate, a thermal grease layer can be provided between the two to increase the contact area and further improve the thermal conductivity. The thermal grease can avoid hard contact between the semiconductor cooler 420 and the heat conducting plate, thereby protecting the semiconductor cooler 420.

[0071] Of course, the heat conducting plate may also be made of heat conducting metal, or may also be made of non-metallic material with good heat conducting properties.

[0072] By changing the direction of the direct current of the semiconductor cooler 420, the end of the semiconductor cooler 420 contacting the carrier 100 can be cooled or heated, thereby heating or cooling. It can be understood that if the power supply is reversed, the temperature of the end of the semiconductor cooler 420 contacting the carrier 100 changes in the opposite direction.

[0073] For example, during cooling, one end of the semiconductor refrigerator 420 in contact with the carrier 100 cools, and the other end of the semiconductor refrigerator 420 away from the carrier 100 heats, and then the heat sink 430 cools the heating end of the semiconductor refrigerator 420 .

[0074] For example, the DC power supply is used to provide a DC current; the DC power supply is electrically connected to the semiconductor refrigerator 420 through a power commutator. The DC transmission direction of the DC power supply flowing into the semiconductor refrigerator 420 is changed by the power commutator. For example, when the DC current flows in the forward direction, the semiconductor refrigerator 420 contacts the end of the carrier 100 for cooling; when the DC current flows in the reverse direction, the semiconductor refrigerator 420 contacts the end of the carrier 100 for heating. The DC power supply can be configured to be formed by converting AC power into DC power, which will not be described in detail.

[0075] like Figure 6 As shown, in some embodiments, the carrier 100 includes a dish tray having a plurality of limiting grooves, and the limiting grooves can accommodate the culture dish 300; wherein the limiting grooves form limiting portions.

[0076] That is to say, the culture dish 300 can be directly placed in the limiting groove, and the culture dish 300 can be limited by the limiting groove, thereby reducing the risk of the culture dish 300 being displaced or dropped.

[0077] For example, the limiting groove may be formed by a recessed area on the dish. Of course, it may also be formed by a raised area on the dish, which is not specifically limited here. Optionally, for example, the dish is a metal plate, and the limiting groove is formed by a sheet metal extrusion process.

[0078] It should be noted that the limiting groove has a cavity, and the cavity can form a low-temperature zone. When the culture dish 300 is placed in the cavity, the low-temperature zone can more accurately control the temperature of the culture dish 300.

[0079] Exemplarily, the culture dish 300 is at least partially located in the limiting groove. Of course, in other embodiments, the upper end of the culture dish 300 can be higher than the upper end of the limiting groove, thereby facilitating the placement of the culture dish 300.

[0080] Exemplarily, in order to facilitate taking and placing the dish tray, the dish tray is provided with a handle 110 .

[0081] like Figure 1 and Figure 2 As shown, in some embodiments, the cold stage assembly further includes a refrigerating unit 200, the refrigerating unit 200 has an inner cavity, the inner cavity has a loading position 240, and the loading position 240 is used to store opened solution bottles 250; wherein, the refrigerating unit 200 has a liquid extraction port 210, and the liquid extraction port 210 is connected to the solution bottle 250 located at the loading position 240.

[0082] That is to say, the solution bottle 250 loaded with culture oil or culture liquid can be opened and placed at the loading position 240 in the inner cavity of the refrigerator 200, so that by configuring the position of the liquid collection port 210 and the loading position 240, the staff can absorb the culture oil or culture liquid through a pipetting tool such as a pipette.

[0083] Obviously, by providing the refrigeration unit 200 and placing the solution bottle 250 in the inner cavity, the temperature of the culture solution or culture oil can be controlled.

[0084] For example, the number of the refrigerating parts 200 is a pair, which are respectively arranged at two opposite ends of the refrigerating end 410, and the pair of refrigerating parts 200 supports form a limiting area, and the dish plate is placed in the limiting area, and the limiting position of the pair of refrigerating parts 200 can prevent the dish plate from falling. Optionally, the pair of refrigerating parts 200 can limit the dish plate to achieve accurate positioning and installation of the dish plate, so as to cooperate with the automatic dripping device to identify the position of the culture dish 300 on the dish plate.

[0085] It should be noted that, by providing a pair of refrigerating parts 200, for respectively placing a solution bottle 250 loaded with culture oil and a solution bottle 250 loaded with culture liquid, confusion and mistakes in taking liquids can be avoided.

[0086] For example, for easy identification, a pair of refrigerating parts 200 can be defined as a first refrigerating part 200 and a second refrigerating part 200, respectively. The first refrigerating part 200 is used to store a culture fluid solution bottle 250, and the second refrigerating part 200 is used to store a culture oil solution bottle 250. The first refrigerating part 200 and the first pipetting component 600 are located on the same side, and the second refrigerating part 200 and the second pipetting component 500 are located on the same side.

[0087] like Figure 7 As shown, in some embodiments, the refrigerator 200 further has a cache location 260 therein, and the cache location 260 is used to store unopened solution bottles 250 .

[0088] That is, by providing a buffer position 260 in the refrigerating part 200 to store the spare solution bottle 250, the solution bottle 250 can be quickly replaced. In other words, after the solution in the solution bottle 250 at the loading position 240 is used up, the solution bottle 250 at the buffer position 260 can be opened and placed at the loading position 240 to ensure normal liquid extraction.

[0089] In some embodiments, the refrigeration unit 200 includes a box body 230 and a rack 270, and the box body 230 has an opening; wherein the refrigeration end 410 is partially located in the box body 230, and the liquid extraction port 210 is arranged in the box body 230, and a loading position 240 and a buffer position 260 are formed in the box body 230; the rack 270 is slidably connected to the box body 230, and the rack 270 can enter and exit the box body 230 from the opening, and the loading position 240 and the buffer position 260 are both arranged on the moving track of the rack 270; wherein the rack 270 is used to place unopened solution bottles 250 and opened solution bottles 250.

[0090] Exemplarily, an opening is provided on the side of the box body 230, and the liquid collection port 210 is provided at the upper end of the box body 230, and the liquid collection port 210 corresponds to the loading area to ensure that the pipette can be inserted from the liquid collection port 210 into the solution bottle 250 located in the loading area to collect liquid.

[0091] Among them, the rack 270 is arranged to be able to slide in and out of the box body 230, so that the rack 270 constitutes a drawer structure, and then by placing the opened solution bottle 250 and the unopened solution bottle 250 on the rack 270, it is possible to control the solution bottle 250 to enter and exit the box body 230 for replacement, and it is possible to control the opened solution bottle 250 to move to the loading position 240, and the unopened solution bottle 250 to move to the cache position 260.

[0092] For example, the opened solution bottle 250 and the unopened solution bottle 250 are manually placed in the buffer position 260 and the loading position 240 of the refrigerator 200, respectively, and the solution bottle 250 is pushed to the corresponding position in the box 230 through the rack 270 to keep the box 230 sealed to a certain extent. Optionally, an end cap 271 is provided at the end of the rack 270, and when the solution bottle 250 is moved into place, the end cap 271 closes the opening.

[0093] like Figure 7 As shown, in some embodiments, the refrigeration unit 200 also includes an identification unit 220, which is installed on the box body 230. The identification unit 220 can obtain bottle body information of the solution bottle 250 located at the upper material position 240; wherein the bottle body information includes one or more of the following: liquid level height information in the solution bottle 250, position information of the solution bottle 250, bottle mouth sealing status information of the solution bottle 250 and label information of the solution bottle 250.

[0094] That is to say, the identification unit 220 can obtain one or more of the liquid level height information in the solution bottle 250, the position information of the solution bottle 250, the bottle mouth sealing status information of the solution bottle 250 and the label information of the label of the solution bottle 250; specifically: when the identification unit 220 identifies the liquid level height information in the solution bottle 250, it can prompt the staff to replace the solution bottle 250 in time; when the identification unit 220 identifies the bottle mouth sealing status of the solution bottle 250, it can check whether the solution bottle 250 is unsealed, so as to avoid the staff from mistakenly placing the unopened solution bottle 250 at the loading position 240; when the identification unit 220 identifies the position information of the solution bottle 250, it can correct the deviation, so as to avoid the problem that the shelf 270 does not move to a certain position, resulting in poor sealing of the box 230 and inconvenience in taking liquid; when the identification unit 220 identifies the label information, for example, it identifies the model and batch of the culture solution or culture oil, so as to prevent the occurrence of mixed liquids, wrong placement or expired solutions.

[0095] Exemplarily, when identifying one or more of the liquid level height information in the solution bottle 250, the position information of the solution bottle 250, the bottle mouth sealing status information of the solution bottle 250, and the label information of the label of the solution bottle 250, the identification unit 220 is a camera; when identifying the liquid level height information in the solution bottle 250, the identification unit 220 is a liquid level sensor, such as a reflected laser sensor.

[0096] In some embodiments, the present application further provides a liquid transfer device, the liquid transfer device comprising:

[0097] N pipetting components, each of which can drip a corresponding target liquid medium into a culture dish 300 located in a preset area; wherein a plurality of culture dishes 300 can be placed in the preset area, and N is a positive integer, and N≥2;

[0098] The cold stage assembly is a liquid preparation cold stage as described in any one of the above embodiments, and the culture dish 300 located on the carrier 100 is located in the preset area.

[0099] This embodiment takes the dripping of culture fluid and culture oil, and the number of pipetting components is 2 as an example. Of course, in other embodiments, it can also be used for dripping of multiple culture oils and multiple culture fluids. Accordingly, the number of pipetting components can be 3, 4, 5, 6, 7, 8, etc., which is not specifically limited here.

[0100] When dripping culture oil and culture solution into the culture dish 300, two pipetting components are allocated to set the first pipetting component 600 for dripping culture solution and the second pipetting component 500 for dripping culture oil. That is to say, the second pipetting component 500 is used to absorb the culture oil and drip it into the culture dish 300, and then the first pipetting component 600 absorbs the culture solution and drips it into the culture dish 300 that has been dripped with culture oil. It should be noted that the first pipetting component 600 and the second pipetting component 500 both have a moving range, and the moving range of the first pipetting component 600 and the moving range of the second pipetting component 500 have an overlapping area, which is equivalent to the first pipetting component 600 and the second pipetting component 500 can both move in the overlapping area and perform the dripping action. It can be seen that the preset area is set in the overlapping area, thereby ensuring that the first pipetting component 600 and the second pipetting component 500 can drip all culture dishes 300 placed in the preset area.

[0101] Obviously, by reasonably setting the area of ​​the preset area, it is possible to arrange and place multiple culture dishes 300 to be dripped at the same time, and then use a pair of pipetting components to replace manual dripping. For example, the pipetting component can use a mechanical arm in conjunction with an automatic pipette structure to drive the automatic pipette to move within the preset area, and perform aspiration or dripping when the automatic pipette moves to the target position. Of course, a lead screw linear mechanism can also be used in conjunction with an automatic pipette structure to arrange multiple culture dishes 300 along the moving path of the automatic pipette gas; or a belt drive mechanism can be used in conjunction with an automatic pipette, etc. Of course, the automatic pipette can be replaced with an electric syringe with a drive unit.

[0102] like Figure 3 and Figure 4 As shown, in some embodiments, the pipetting component includes a pipetting member 530, a first driving member 520 and a second driving member 510, and the pipetting member 530 can absorb and discharge the target liquid medium; the first driving member 520 is connected to the pipetting member 530, and the first driving member 520 can drive the pipetting member 530 to move in a first direction; the second driving member 510 is connected to the first driving member 520, and the second driving member 510 is used to drive the first driving member 520 to move in a first reference plane; wherein the first direction and the first reference plane are vertically arranged, and the first reference plane and the storage table are parallel.

[0103] Exemplarily, the pipette 530 is a pipette, which can be used to absorb and drip liquid media. In this embodiment, the pipette is located above the storage table, and the mouth of the pipette faces downward, so that the height of the pipette can be adjusted by the first driving member 520 to control the mouth of the pipette to enter and exit the culture dish 300.

[0104] Exemplarily, the first driving member 520 is a linear moving mechanism, such as an electric push rod, a screw moving mechanism, a pneumatic cylinder, a hydraulic cylinder, etc., which is not specifically limited here, as long as the height of the pipetting member 530 can be adjusted.

[0105] Exemplarily, the second driving member 510 may be a robotic arm, and the pipette is installed at the end of the robotic arm, wherein the robotic arm may be a four-axis robotic arm, a six-axis robotic arm, a seven-axis robotic arm, etc.

[0106] It is easy to understand that in this embodiment, the second driving member 510 is a four-axis robot, so that the first driving member 520 and the second driving member 510 constitute a five-axis robot, which can enable the pipette to move in a three-dimensional space. Specifically, the second driving member 510 can drive the pipette to move in a horizontal plane, and the first driving member 520 can drive the pipette to move in a vertical direction.

[0107] like Figure 3 and Figure 4As shown, in some embodiments, the pipetting device also includes M cover opening components 800, at least one pipetting component among the N pipetting components is connected to two cover opening components 800, M is a positive integer, and M≥2; wherein, the cover opening component 800 includes a cover opening member 820 and a third driving member 810, and the cover opening member 820 can grab the dish lid of the culture dish 300; the third driving member 810 is connected to the cover opening member 820, and the third driving member 810 is connected to the second driving member 510, the second driving member 510 is used to drive the second driving member 510 to move in the first reference plane, and the third driving member 810 is used to drive the cover opening member 820 to move in the first direction.

[0108] In this embodiment, the number of the pipetting components is two, and the two pipetting components are respectively provided with a first pipetting component 600 for dripping the culture solution and a second pipetting component 500 for dripping the culture oil, and two cover opening components 800 are provided on the second pipetting component 500. That is, the cover of the culture dish 300 can be taken and placed by using the cover opening component 800, so that the culture dish 300 can be opened for dripping before dripping, and the cover can be closed after the dripping is completed.

[0109] It is easy to understand that, by way of example, a specific working process includes the following steps:

[0110] Step 1: manually placing the dish tray filled with culture dishes 300, the cold table assembly and the refrigerating part 200 on the table, and placing the dish tray on the cold table assembly, and placing two opened culture liquid solution bottles 250 and culture oil solution bottles 250 into the loading positions 240 of the refrigerating part 200 storing culture liquid and the refrigerating part 200 storing culture oil, respectively, and placing the other two unopened culture liquid solution bottles 250 and culture oil solution bottles 250 into the buffer positions 260 of the refrigerating part 200 storing culture liquid and the refrigerating part 200 storing culture oil, respectively;

[0111] Step 2: The first driving member 520 of the first pipetting component 600 cooperates with the second driving member 510 to drive the recognition component 700 to move, and the recognition component 700 is used to obtain the image information of the dish cover of the first culture dish 300;

[0112] Step 3: The first driving member 520 of the first pipetting assembly 600 cooperates with the second driving member 510 to drive the pipetting member 530 to move to the refrigerating member 200 storing the culture solution to absorb the culture solution; at the same time, the second pipetting assembly 500 moves, and the pipetting member 530 of the second pipetting assembly 500 is controlled to absorb the culture oil in the culture oil solution bottle 250 in the upper material position 240;

[0113] Step 4: Using the cover opening member 820 of the first cover opening assembly 800 in cooperation with the third driving member 810 to grab the cover of the first culture dish 300, and using the recognition assembly 700 to take a photo of the bottom of the first culture dish 300 to determine whether the culture oil is dripped into the second culture dish 300;

[0114] Step 5: The second pipetting component 500 drips the culture oil into the first culture dish 300 according to the photographing result; at the same time, the first pipetting component 600 moves with the cover opening component 800 to the top of the second culture dish 300, the recognition component 700 takes a picture of the dish cover of the second culture dish 300, and the cover opening member 820 of the second cover opening component 800 cooperates with the third driving member 810 to grab the dish cover of the second culture dish 300, and the recognition component 700 takes a picture of the dish bottom of the second culture dish 300 to determine whether the culture oil is dripped into the second culture dish 300;

[0115] Step 6: The first pipetting component 600 exchanges positions with the second pipetting component 500;

[0116] Step 7: The second pipetting component 500 drips culture oil into the second culture dish 300 according to the photographing result; at the same time, the first pipetting component 600 drips culture fluid into the first culture dish 300, and the first cover opening component 800 covers the lid of the first culture dish 300 on the first culture dish 300, the second pipetting component 500 drives the first cover opening component 800 to the third culture dish 300 to take pictures and grab the lid of the third culture dish 300, the first pipetting component 600 moves to the culture fluid solution bottle 250 to take liquid and drips culture fluid into the second culture dish 300, the second pipetting component 500 moves to the culture oil solution bottle 250 to take liquid and drips culture oil into the third culture dish 300; the operation is repeated in sequence until all culture dishes 300 are dripped.

[0117] It should be noted that the first pipetting component 600 and the second pipetting component 500 are respectively located at two opposite ends of the cold stage component, which can avoid position interference between the first pipetting component 600 and the second pipetting component 500.

[0118] In summary, the efficiency of the single-channel pipetting solution can be improved to the extreme without causing mutual interference. The single-channel pipetting solution refers to dripping the culture solution and the culture oil by the first pipetting component 600 cooperating with the second pipetting component 500.

[0119] Exemplarily, the third driving member 810 is a linear motion mechanism, such as an electric push rod, a pneumatic cylinder, a hydraulic cylinder, and the like.

[0120] For example, the cover opening member 820 is a negative pressure suction cup. Of course, in other embodiments, it can also be set as an electric clamp or the like.

[0121] like Figure 3 and Figure 4 As shown, in some embodiments, the pipetting device also includes an identification component 700, which is electrically connected to the pipetting component and the lid opening component 800 respectively, the second driving member 510 is connected to the identification component 700, and the third driving member 810 is used to drive the identification component 700 to move within the first reference plane, and the identification component 700 can obtain the dish body information of the current culture dish 300, and control the pipetting component to switch between executing the dripping action and controlling the lid opening component 800 to execute the dish cover picking and placing action according to the dish body information; wherein the dish body information includes the first information of the dish cover of the culture dish 300 and the second information of the dish bottom of the culture dish 300.

[0122] Exemplarily, the recognition component 700 includes a first camera and a controller, and the first camera is electrically connected to the pipetting component and the lid opening component 800 through the controller. The first camera is used to obtain images of the dish cover and the dish bottom, and can obtain first information of the dish cover and second information of the dish bottom through the controller, so that the controller can perform the actions of taking and placing the dish cover and dripping according to the first information and the second information.

[0123] For example, the bottom and cover of each culture dish 300 have corresponding marks to ensure that the cover can be covered on the corresponding bottom of the dish later. For example, the mark can be a digital number. Of course, in other embodiments, the mark can also be a QR code, an icon, etc.

[0124] In addition, by acquiring the second information from the bottom of the dish, the second information can determine whether the culture solution is dripped. If the culture solution is dripped, the controller controls the second pipetting component 500 to drip the culture oil; if the culture solution is not dripped, that is, it is identified that there are no droplets at the bottom of the dish, the controller controls the first pipetting component 600 to drip the culture solution.

[0125] Exemplarily, the controller performs basic logic control functions, which are not specifically limited herein, such as a PLC programmable controller.

[0126] Exemplarily, the first camera and the controller are installed on the first frame 900, and the first camera faces downward to be able to align with the culture dish 300 below. Among them, the first frame 900 is provided with a fill light to ensure the quality of the captured picture.

[0127] For example, the fill light is arranged in a ring shape, and the first camera can obtain the image of the culture dish 300 below through the inner hole of the ring fill light.

[0128] In some embodiments, the pipetting assembly, cold stage assembly, and identification assembly 700 are all installed on a workbench, and the workbench is also provided with a sealing cover. The pipetting assembly, cold stage assembly, and identification assembly 700 are all located in the sealing cover, and the sealing cover is equipped with an air filter to purify the air and avoid contaminating the culture dish 300.

[0129] For example, the sealing cover may be a transparent structure, so that the outside world can observe the working conditions of the pipetting component, the cold stage component, and the identification component 700 in the sealing cover.

[0130] For example, a waste bin and a gun tip box are provided in the sealing cover. The waste bin can collect the gun tips used by the automatic pipette for centralized processing. The gun tip box can be used to replace the gun tip of the pipette. The pipette uses a tension sleeve, and the size of the gun tip adapter is controlled by a pump to control the opening and closing of the tension sleeve. When the tension sleeve retracts, the diameter of the gun tip adapter is smaller than the inner diameter of the gun tip. Relying on the action of gravity, the gun tip automatically falls to complete the replacement of the gun tip.

[0131] For example, casters are installed at the bottom of the workbench to facilitate movement.

[0132] In all examples shown and described herein, any specific values ​​should be interpreted as merely exemplary and not as limiting, and thus other examples of the exemplary embodiments may have different values.

[0133] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0134] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A liquid-ready cooling table, characterized in that: The liquid standby cooling station comprises: A carrier, the carrier is used to place a plurality of culture dishes, and the carrier is a heat-conducting structure with heat-conducting capability; A cold stage assembly, wherein the cold stage assembly has a storage table, and the storage table is detachably connected to the carrier; wherein the cold stage assembly can adjust the temperature of the carrier located on the storage table.

2. The liquid standby cooling stage according to claim 1, characterized in that: The cold stage assembly comprises: A temperature regulating member is detachably connected to the supporting member, and the temperature regulating member can regulate the temperature of the supporting member.

3. The liquid standby cooling stage according to claim 2, characterized in that: The temperature regulating member comprises: A semiconductor refrigerator, wherein the semiconductor refrigerator has a cooling end, the cooling end abuts against the supporting member, and the cooling end is used to place the supporting member.

4. The liquid standby cooling stage according to claim 3, characterized in that: The carrier comprises: The dish tray has a plurality of limiting grooves, and the limiting grooves can accommodate the culture dish.

5. The liquid standby cooling stage according to claim 4, characterized in that: The cold stage assembly further comprises: A refrigerating unit, wherein the refrigerating unit has an inner cavity, wherein the inner cavity has a loading position and a buffer position, wherein the loading position is used to store opened solution bottles, and the buffer position is used to store unopened solution bottles; wherein the refrigerating unit has a liquid collection port, wherein the liquid collection port is connected to the solution bottle located at the loading position.

6. The liquid standby cooling stage according to claim 5, characterized in that: The refrigeration unit comprises: A box body, the box body having an opening; wherein the refrigeration end portion is located in the box body, and the liquid taking port is arranged in the box body, so that the loading position and the buffer position are formed in the box body; A storage rack, the storage rack is slidably connected to the box body, the storage rack can enter and exit the box body from the opening, and the loading position and the buffer position are both arranged on the moving track of the storage rack; wherein the storage rack is used to place the unopened solution bottles and the opened solution bottles.

7. The liquid standby cooling stage according to claim 6, characterized in that: The refrigeration unit also includes: An identification unit, the identification unit is installed on the box body, the identification unit is electrically connected to the liquid transfer component, and the identification unit can obtain the bottle body information of the solution bottle located at the loading position, and control the liquid transfer component to send a control instruction according to the bottle body information of the solution bottle; The bottle body information includes one or more of the following: liquid level information in the solution bottle, position information of the solution bottle, bottle mouth sealing status information of the solution bottle, and label information of the solution bottle.

8. A liquid transfer device, characterized in that: The liquid transfer device comprises: N pipetting components, each of which can drip a corresponding target liquid medium into a culture dish located in a preset area; wherein a plurality of culture dishes can be placed in the preset area, and N is a positive integer, and N≥2; A cold stage assembly, wherein the cold stage assembly is a liquid preparation cold stage as claimed in any one of claims 1 to 7, and the culture dish located on the carrier is located in the preset area.

9. The liquid transfer device according to claim 8, characterized in that: The pipetting assembly comprises: A pipetting element, which is capable of sucking and discharging the target liquid medium; A first driving member, the first driving member is connected to the liquid transfer member, and the first driving member can drive the liquid transfer member to move in a first direction; A second driving member, the second driving member is connected to the first driving member, and the second driving member is used to drive the first driving member to move in a first reference plane; wherein the first direction and the first reference plane are arranged perpendicularly, and the first reference plane and the storage table are parallel.

10. The liquid transfer device according to claim 9, characterized in that: The pipetting device further includes M cover opening components, at least one of the N pipetting components is connected to two cover opening components, M is a positive integer, and M≥2; wherein the cover opening component includes: A cover opening member, wherein the cover opening member is capable of grabbing the lid of the culture dish; A third driving member, the third driving member is connected to the cover opening member, and the third driving member is connected to the second driving member, the second driving member is used to drive the second driving member to move within the first reference plane, and the third driving member is used to drive the cover opening member to move in the first direction.

11. The liquid transfer device according to claim 10, characterized in that: The liquid preparation cold stage also includes an identification component, which is electrically connected to the pipetting component and the lid opening component respectively, and the second driving member is connected to the identification component, and the second driving member is used to drive the identification component to move within the first reference plane, and the identification component can obtain the current dish body information of the culture dish, and control the pipetting component to switch between executing the dripping action and controlling the lid opening component to execute the action of taking and placing the dish cover according to the dish body information; wherein the dish body information includes the first information of the dish cover of the culture dish and the second information of the dish bottom of the culture dish.

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