Construction method of somatic cell library

Abstract

The invention discloses a construction method of a somatic cell bank, which belongs to the technical field of somatic cell banks, and comprises the following steps: collection and identification of cell strains, culture and passage of the cell strains, cryopreservation and preservation of the cell strains, cell quality control and detection, updating and recovery of the cell bank, and safety management of the cell bank. The construction efficiency of the cell bank is improved by optimizing the steps of cell culture, passage, cryopreservation and the like, the stability and the activity of cells in the long-term storage process are ensured by adopting an advanced freezing technology and a preservation system, a perfect cell bank information management system is established, detailed information of each cell strain is recorded and managed, and the cell bank management efficiency is improved. The traceability reliability of the cells is ensured, a cell bank safety management system is established, and a plurality of measures are adopted to protect cell resources in the cell bank from being lost and damaged.

Description

Technical Field

[0001] The present invention relates to the technical field of somatic cell banks, and in particular to a method for constructing a somatic cell bank. Background Art

[0002] With the in-depth development of biomedical research, cell banks, as important biological resources, play an important role in drug screening, gene therapy, disease model construction, etc. However, traditional cell bank construction methods have problems such as low purity, poor activity, and insufficient stability, which limit their application in the field of biomedicine. Therefore, it is of great practical significance to develop a method for constructing a somatic cell bank with high purity, high activity, and high stability. Summary of the invention

[0003] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a method for constructing a somatic cell bank.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] Step 1: Collection and identification of cell lines;

[0006] Step 2: Cultivation and subculturing of cell lines;

[0007] Step 3: Cryopreservation and storage of cell lines;

[0008] Step 4: Cell quality control and testing;

[0009] Step 5: Renewal and recovery of cell bank;

[0010] Step 6: Safe management of cell banks.

[0011] As a further description of the above technical solution:

[0012] The collection and identification in step 1 should select cell lines with clear sources and good growth for collection, and identify them through molecular biology techniques to ensure the purity and availability of the cells.

[0013] As a further description of the above technical solution:

[0014] The culture and subculture in step 2 should be carried out under sterile, constant temperature and constant humidity conditions, and the cell line should be cultured and subcultured regularly to maintain the activity and stability of the cells.

[0015] As a further description of the above technical solution:

[0016] The freezing and preservation in step 3 should be carried out by freezing the cell line in combination with a suitable cryoprotectant.

[0017] As a further description of the above technical solution:

[0018] The quality control and testing in step 4 should be to test the cell activity, purity, genetic stability, etc. of each batch of cell lines.

[0019] As a further description of the above technical solution:

[0020] The updating and recovery in step five should be to establish a complete cell bank information management system to record and manage the detailed information of each cell line.

[0021] As a further description of the above technical solution:

[0022] The safety management in step five should be to take multiple measures to protect the cell resources in the cell bank from loss and damage.

[0023] The present invention has the following beneficial effects:

[0024] In the present invention, the efficiency of cell bank construction is improved by optimizing the steps of cell culture, passage and cryopreservation, and advanced freezing technology and preservation system are adopted to ensure the stability and activity of cells during long-term storage. A complete cell bank information management system is established to record and manage the detailed information of each cell line to ensure the reliable traceability of cells, establish a cell bank safety management system, and take various measures to protect the cell resources in the cell bank from loss and damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The present invention is a flowchart of a method for constructing a somatic cell bank. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Reference Figure 1 , an embodiment provided by the present invention:

[0028] Step 1: Collection and identification of cell lines;

[0029] Step 2: Cultivation and subculture of cell lines;

[0030] Step 3: Cryopreservation and storage of cell lines;

[0031] Step 4: Cell quality control and testing;

[0032] Step 5: Renewal and recovery of cell bank;

[0033] Step 6: Safe management of cell banks.

[0034] The collection and identification in step 1 should select cell lines with clear sources and good growth for collection, and identify them through molecular biology techniques to ensure the purity and availability of the cells.

[0035] The culture and subculture in step 2 should be carried out under sterile, constant temperature and constant humidity conditions, and the cell lines should be cultured and subcultured regularly to maintain the activity and stability of the cells.

[0036] The freezing and preservation in step three should be combined with appropriate cryoprotectants to freeze the cell lines.

[0037] The quality control and testing in step 4 should be to test the cell activity, purity, genetic stability, etc. of each batch of cell lines.

[0038] The update and recovery in step five should be to establish a complete cell bank information management system to record and manage the detailed information of each cell line.

[0039] The safety management in step five should be to take multiple measures to protect the cell resources in the cell bank from loss and damage.

[0040] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for constructing a somatic cell bank, characterized in that: The following steps are involved: Step 1: Collection and identification of cell lines; Step 2: Cultivation and subculture of cell lines; Step 3: Cryopreservation and storage of cell lines; Step 4: Cell quality control and testing; Step 5: Renewal and recovery of cell bank; Step 6: Safe management of cell banks.

2. The method for constructing a somatic cell bank according to claim 1, characterized in that: The collection and identification in step 1 should select cell lines with clear sources and good growth for collection, and identify them through molecular biology techniques to ensure the purity and availability of the cells.

3. The method for constructing a somatic cell bank according to claim 1, characterized in that: The culture and subculture in step 2 should be carried out under sterile, constant temperature and constant humidity conditions, and the cell line should be cultured and subcultured regularly to maintain the activity and stability of the cells.

4. The method for constructing a somatic cell bank according to claim 1, characterized in that: The freezing and preservation in step 3 should be combined with a suitable cryoprotectant to freeze the cell line.

5. The method for constructing a somatic cell bank according to claim 1, characterized in that: The quality control and testing in step 4 should be to test the cell activity, purity, genetic stability, etc. of each batch of cell lines.

6. The method for constructing a somatic cell bank according to claim 1, characterized in that: The updating and recovery in step five should be to establish a complete cell bank information management system to record and manage the detailed information of each cell line.

7. The method for constructing a somatic cell bank according to claim 1, characterized in that: The safety management in step five should be to take multiple measures to protect the cell resources in the cell bank from loss and damage.

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