Separated noise reduction and shock absorption optical platform for patch clamp experiment

Abstract

The invention discloses a separated noise reduction and shock absorption optical platform for patch clamp experiments. The separated noise reduction and shock absorption optical platform comprises a buoyancy tank body, a substrate platform and an operation platform, a movable bottom plate is arranged at the inner bottom of the buoyancy tank body, the annular side of the movable bottom plate abuts against the inner wall of the buoyancy tank body to form sealing, and a damping spring piece is arranged on the base plate platform and penetrates through the bottom of the buoyancy tank body. A bent rod is fixedly arranged on the bottom side of the movable bottom plate, a vertical rod is hinged to one end of the bent rod, a swing arm is hinged to one end of the vertical rod, a driving tooth rotating along with the swing arm is arranged at the end of the swing arm, a driven tooth is arranged on the second supporting rod, a linkage rod is arranged on one side of the driven tooth, one end of the linkage rod is fixedly connected with the movable bottom plate, and the driving tooth and the rack are meshed with the driven tooth. The movable bottom plate and the sealing plate are in a linkage state, fluid in the branch cavity directly enters / flows out of the second hole, the volume of the fluid reserved in the first hole is not affected, the height of the position of the floating plate in the first hole is stable, and interference of signal noise on patch clamp experiments in the experiment process is avoided.

Description

Technical Field

[0001] The invention relates to the technical field of operating platforms for patch clamp experiments, and in particular to a separated noise reduction and vibration reduction optical platform for patch clamp experiments. Background Art

[0002] Patch clamp technology is an electrophysiological research tool that can reveal the electrophysiological characteristics of cells by recording and analyzing ion channels on cell membranes, providing important support for research in multiple fields such as neuroscience, pharmacology, and cell biology.

[0003] The patch clamp optical experimental platform is an experimental system that combines patch clamp technology and optical imaging technology. It is used to study the physiological functions of cells and ion channels, and can simultaneously measure the electrophysiological and optical properties of cell membranes. By performing patch clamp experiments, the current passing through ion channels or the potential changes of cell membranes can be measured; through optical imaging technology, the structure and function of cells can be observed. However, the patch clamp experimental operation platform has the problem of signal noise during use. Mechanical vibrations, traffic vibrations, and building vibrations inside and outside the laboratory will be transmitted to the patch clamp experimental device through the experimental platform, causing signal noise. The mechanical noise of the equipment itself, such as mechanical vibrations generated during the operation of microscopes, micromanipulators, pumps, etc., may also become a source of signal noise.

[0004] The microcurrent measurement in the patch clamp experiment is usually very sensitive, so it is necessary to provide a reliable patch clamp experimental platform to ensure the accuracy and reliability of the experiment. Summary of the invention

[0005] The purpose of the present invention is to provide a separate noise reduction and vibration reduction optical platform for patch clamp experiments to solve the technical problem that during the patch clamp experiment, the vibration in the environment is transmitted to the experimental platform, causing signal noise to interfere with the accuracy and reliability of the experimental results.

[0006] In order to solve the above technical problems, the present invention specifically provides the following technical solutions:

[0007] The present invention provides a separated noise reduction and vibration reduction optical platform for patch clamp experiments, comprising: a buoyancy tank body, a partition is fixedly arranged inside the buoyancy tank body, the partition is arranged along the cross section of the buoyancy tank body, the partition divides the inner cavity of the buoyancy tank body into a sub-cavity located below the partition, and the sub-cavity is used to fill with fluid; a first hole and a second hole are opened through the partition, a movable sealing plate is arranged in the second hole, and the fluid in the sub-cavity can be pressed into the first hole and the second hole by external pressure; a movable bottom plate is arranged at the inner bottom of the buoyancy tank body, and a seal is formed between the ring side of the movable bottom plate and the inner wall of the buoyancy tank body; a substrate platform, the outer bottom of the buoyancy tank body is fixed on the substrate platform by a shock absorbing column support, a damping spring member is arranged on the substrate platform and passes through the bottom of the buoyancy tank body, and the damping spring member supports the movable bottom plate to contact with the inner bottom of the buoyancy tank body Separation, the vibration received by the substrate platform is weakened by the damping column and then transmitted to the buoyancy tank body and absorbed by the fluid in the sub-cavity; an operating platform, the operating platform is arranged above the buoyancy tank body and separated from it, a floating base column is fixedly connected to the bottom of the operating platform, a floating plate is fixedly connected to the bottom of the floating base column, and the floating plate closes the first hole and is movably arranged therein; a bent rod is fixedly provided on the bottom side of the movable bottom plate, one end of the bent rod is hinged with a vertical rod, and one end of the vertical rod is hinged with a swing arm, a support rod 1 is provided on the outer wall of the buoyancy tank body, the support rod 1 is axially connected to one end of the swing arm, and an active tooth rotating therewith is provided at the end of the swing arm, a support rod 2 is fixedly provided on the support rod 1, a driven tooth is provided on the support rod 2, a linkage rod is provided on one side of the driven tooth, one end of the linkage rod is fixedly connected to the movable bottom plate, and the other end of the linkage rod is provided with a rack, and the active tooth and the rack are respectively meshed with the driven tooth.

[0008] Furthermore, the first hole is arranged in the middle of the partition, and the second hole is arranged on an annular path around the first hole.

[0009] Furthermore, the area of ​​the movable bottom plate is S, the area of ​​the first hole is S1, the area of ​​the second hole is S2, and the transmission ratio of the driving tooth and the driven tooth is S2:S, so that the volume change of the fluid in the sub-cavity is consistent with the volume change of the fluid in the second hole.

[0010] Furthermore, the end of the swing arm is key-connected to the driving tooth.

[0011] Furthermore, a limit stop is provided on the inner wall of the second hole, and the limit stop is arranged close to the lower port of the second hole; wherein a limit slot is opened on the sealing plate, the limit slot is located on the lower surface of the sealing plate, and the limit stop corresponds to the position of the limit slot so as to be embedded therein; the limit slot is opened at equal intervals in the circumferential direction at the edge of the sealing plate, the number of the limit stops and the limit slots is at least 2, and the number of the limit slots is not less than the limit stops.

[0012] Furthermore, a telescopic rod is provided on the upper surface of the sealing plate, and the telescopic rod is arranged close to the edge of the sealing plate. The guide rod is used to keep the sealing plate in a floating path in the vertical direction; one end of the telescopic rod is fixedly connected to the sealing plate, and the other end of the telescopic rod is arranged on the limit stop block.

[0013] Furthermore, a connecting hole is provided on the limit stopper, in which a slidable top block and a limiting body arranged above the top block are provided, and the top block is used to connect the telescopic rod; as the telescopic rod is subjected to force, the top block gradually slides upward and stops at the limiting body, and the base of the telescopic rod retracts into the connecting hole.

[0014] Furthermore, the top surface of the floating base column is higher than the top edge of the buoyancy tank body, so that the operating platform and the buoyancy tank body are in a separated state.

[0015] Furthermore, the partition is arranged at a middle height position of the inner cavity of the buoyancy tank.

[0016] Furthermore, a shock absorbing ring is sleeved on the shock absorbing column. The shock absorbing ring is arranged close to the bottom of the shock absorbing column. The shock absorbing ring is connected to the upper surface of the substrate platform to reduce the vibration transmitted from the substrate platform to the shock absorbing column.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] 1. The present invention can separate the experimental operation platform from the substrate platform for placing the equipment by setting the buoyancy tank, and utilize the buoyancy generated by the fluid material filled in the buoyancy tank to provide floating support for the operation platform, thereby avoiding direct or indirect contact between the operation platform and the substrate platform, and also greatly reducing the energy transmitted from the vibration on the substrate platform to the operation platform, thereby avoiding the signal noise caused during the experiment from interfering with the patch clamp experiment, thereby ensuring the smooth progress of the patch clamp experiment to the greatest extent, and solving the problem that the patch clamp experiment operation platform has signal noise during use and affects the microcurrent;

[0019] 2. The present invention uses meshing transmission between active teeth and driven teeth to put the movable bottom plate and the sealing plate in a linkage state, so that the movable bottom plate can drive the sealing plate to move during vibration, so that the fluid in the sub-cavity directly enters / flows out of the second hole, while the volume of the fluid retained in the first hole is not affected, so that the position of the floating plate in the first hole is highly stable, thereby improving the stability of the experimental platform. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0021] Figure 1 A three-dimensional structural diagram of a separate noise reduction and vibration reduction optical platform for patch clamp experiments provided by the present invention;

[0022] Figure 2 for Figure 1 A cross-sectional view of

[0023] Figure 3 for Figure 2 Enlarged view of middle Ⅰ;

[0024] Figure 4 for Figure 2 Enlarged view of middle Ⅱ;

[0025] Figure 5 for Figure 2 Enlarged view of middle III;

[0026] Figure 6 for Figure 5 A structural schematic diagram and a partial view thereof in another embodiment of the present invention.

[0027] The numbers in the figure represent the following:

[0028] 1. Buoyancy tank; 11. partition; 12. sub-cavity; 13. first hole; 14. second hole; 141. limit block; 15. sealing plate; 151. limit slot; 16. movable bottom plate; 161. bent rod; 162. vertical rod; 163. swing arm; 164. support rod one; 165. active gear; 166. support rod two; 167. driven gear; 168. linkage rod; 169. rack; 17. connecting hole; 171. top block; 172. limit body; 18. limit ring; 2. base plate platform; 21. shock absorbing column; 22. damping spring member; 23. shock absorbing ring; 3. operating platform; 31. floating base column; 32. floating plate; 33. telescopic rod member. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] like Figure 1-3 As shown, the present invention provides a separate noise reduction and vibration reduction optical platform for patch clamp experiments, comprising: a buoyancy tank 1, a partition 11 is fixedly arranged inside the buoyancy tank 1, the partition 11 is arranged along the cross section of the buoyancy tank 1, the partition 11 divides the inner cavity of the buoyancy tank 1 into a sub-cavity 12 located below the partition 11, and the sub-cavity 12 is used to fill a fluid; a first hole 13 and a second hole 14 are formed through the partition 11, a movable sealing plate 15 is arranged in the second hole 14, and the fluid in the sub-cavity 12 can be pressed into the first hole 13 and the second hole 14 by external pressure; a movable bottom plate 16 is arranged at the inner bottom of the buoyancy tank 1, and a seal is formed between the ring side of the movable bottom plate 16 and the inner wall of the buoyancy tank 1;

[0031] The outer bottom of the buoyancy tank 1 is supported and fixed on the substrate platform 2 by a damping column 21. A damping spring member 22 is provided on the substrate platform 2 and penetrates the bottom of the buoyancy tank 1. The damping spring member 22 supports the movable bottom plate 16 to be separated from the inner bottom of the buoyancy tank 1. The vibration received by the substrate platform 2 is weakened by the damping column 21 and then transmitted to the buoyancy tank 1 and absorbed by the fluid in the sub-cavity 12.

[0032] The operating platform 3 is arranged above the buoyancy tank 1 and is separated therefrom. A floating base column 31 is fixedly connected to the bottom of the operating platform 3. A floating plate 32 is fixedly connected to the bottom of the floating base column 31. The floating plate 32 closes the first hole 13 and is movably arranged therein.

[0033] More specifically, a bent rod 161 is fixedly provided on the bottom side of the movable bottom plate 16, one end of the bent rod 161 is hingedly connected to a vertical rod 162, one end of the vertical rod 162 is hingedly connected to a swing arm 163, a support rod 164 is provided on the outer wall of the buoyancy tank 1, the support rod 164 is axially connected to one end of the swing arm 163, the end of the swing arm 163 is provided with a driving tooth 165 that rotates with it, a support rod 2 166 is fixedly provided on the support rod 164, a driven tooth 167 is provided on the support rod 2 166, a linkage rod 168 is provided on one side of the driven tooth 167, one end of the linkage rod 168 is fixedly connected to the movable bottom plate 16, the other end of the linkage rod 168 is provided with a rack 169, and the driving tooth 165 and the rack 169 are respectively meshed with the driven tooth 167. The end of the swing arm 163 and the driving tooth 165 are key-connected.

[0034] The present invention provides a separate noise reduction and vibration reduction optical platform for patch clamp experiments. Through the setting of the buoyancy tank 1, the experimental operation platform 3 can be separated from the substrate platform 2 for placing equipment. The buoyancy generated by the fluid material filled in the buoyancy tank 1 is used to float and support the operation platform 3, avoiding direct or indirect contact between the operation platform 3 and the substrate platform 2, and greatly reducing the energy transmitted from the vibration on the substrate platform 2 to the operation platform 3. The fluid material that can be selected in the buoyancy tank 1 is hydraulic oil, silicone oil, etc. The device can block mechanical vibrations from inside and outside the laboratory, traffic vibrations, building vibrations, and mechanical vibrations generated during the operation of experimental equipment such as microscopes, micromanipulators, pumps, etc., to avoid transmission through the substrate platform 2 or other experimental platforms to the operation platform 3 or other operating devices of the patch clamp experiment, thereby avoiding interference of signal noise caused during the experiment on the patch clamp experiment, thereby ensuring the smooth progress of the patch clamp experiment to the greatest extent, and solving the problem that the patch clamp experiment operation platform has signal noise during use and affects the microcurrent.

[0035] Specifically, the outer shell of the buoyancy tank 1 is supported by the shock-absorbing column 21. First, the vibration transmitted to the substrate platform 2 is absorbed and weakened by the vibration-absorbing effect of the shock-absorbing column 21. Most of the weakened vibration energy is transmitted through the side wall of the buoyancy tank 1 to the fluid material in the sub-cavity 12 formed inside the buoyancy tank 1. The fluid material is used to absorb the vibration energy, which largely avoids the transmission of vibration energy to the operating platform 3, which is beneficial to improving the accuracy of the patch clamp experiment results.

[0036] On the other hand, a portion of the vibration energy will be transmitted from the damping spring member 22 to the movable bottom plate 16, causing the movable bottom plate 16 to vibrate. During the up / down vibration of the movable bottom plate 16, the swing arm 163 is first driven to swing through the bent rod 161 and the vertical rod 162. While the swing arm 163 swings, the active tooth 165 rotates accordingly, and then the rotation of the driven tooth 167 drives the rack 169 to rise / fall, and finally the sealing plate 15 is driven to rise / fall through the linkage rod 168, so as to suck / discharge the fluid in the sub-cavity 12, which helps to reduce the change in the liquid level in the first hole 13 and reduce the floating amplitude of the floating plate 32 and the floating base column 31 when they are vibrated.

[0037] Based on the above embodiment, more specifically, the first hole 13 is provided in the middle of the partition 11, and the second hole 14 is provided on the annular path around the first hole 13. The area of ​​the movable bottom plate 16 is S, the area of ​​the first hole 13 is S1, the area of ​​the second hole 14 is S2, and the transmission ratio of the active tooth 165 and the driven tooth 167 is S2:S, so that the volume change of the fluid in the sub-chamber 12 is consistent with the volume change of the fluid in the second hole 14.

[0038] In the present embodiment, the sub-cavity 12, the first hole 13, and the second hole 14 are all in a vacuum state. Under normal circumstances, the change in the volume of the fluid in the sub-cavity 12 will directly affect the positions of the floating plate 32 and the sealing plate 15. In order to avoid the change in the liquid level in the first hole 13 and make the floating plate 32 in a relatively stable state, the present embodiment adopts a linkage between the movable bottom plate 16 and the sealing plate 15, so that the movable bottom plate 16 can drive the sealing plate 15 to move during the vibration process, so that the fluid in the sub-cavity 12 directly enters / flows out of the second hole 14, while the volume of the fluid retained in the first hole 13 is not affected, so that the position of the floating plate 32 is stable.

[0039] Specifically, if S:S1:S2=10:4:1, the vibration of the movable bottom plate 16 causes the liquid level in the sub-cavity 12 to rise by h, and the volume of the fluid entering the first hole 13 and the second hole 14 is 10h. According to the principle of communicating vessels, under normal circumstances, the liquid level rises in the first hole 13 and the second hole 14 by h1=10h / (S1+S2), so h1=2, and the sealing plate 15 and the floating plate 32 move together at this time; if the fluid with a volume of 10h only enters the second hole 14, the liquid level rises in the second hole 14 by h2=10h / S2, h2=10, and h:h2=S2:S=1:10, and the sealing plate 15 moves and the floating plate 32 does not move. That is, the movable height of the sealing plate 15 and the movable bottom plate 16 in the linkage state is inversely proportional to their areas. Since the movable bottom plate 16 is linked with the active tooth 165 and the sealing plate 15 is linked with the driven tooth 167, the sealing plate 15 moves 10 distances for every movement of the movable bottom plate 16, so the transmission ratio of the active tooth 165 and the driven tooth 167 should be 1:10. Therefore, in the reciprocating cycle of the up / down vibration of the movable bottom plate 16, the liquid level in the sub-cavity 12 can flow in / out only from the second hole 14 in a fluctuating state, while the liquid level in the first hole 13 remains basically unchanged.

[0040] In the above embodiment, since there is no necessary limiting structure in the second hole 14 below the sealing plate 15, the sealing plate 15 is easy to slip out of the second hole 14 when it moves downward. In order to solve the above problem, the present invention provides a limiting structure to limit the range of movement of the sealing plate 15 when it moves downward, such as Figure 5-6As shown, a limit stopper 141 is provided on the inner wall of the second hole 14, and the limit stopper 141 is arranged close to the lower end of the second hole 14; wherein, a limit slot 151 is provided on the sealing plate 15, and the limit slot 151 is located on the lower surface of the sealing plate 15, and the limit stopper 141 corresponds to the position of the limit slot 151 so as to be embedded therein; the limit slot 151 is provided at equal intervals in the circumferential direction at the edge of the sealing plate 15, and the number of the limit stopper 141 and the limit slot 151 is at least 2, and the number of the limit slot 151 is not less than the limit stopper 141. In this embodiment, by setting the limit structure, the sealing plate 15 is not easy to fall out of the second hole 14, and the stability of the sealing plate 15 during movement is improved.

[0041] Based on the above embodiments, more specifically, please continue to refer to Figure 5-6 As shown, a telescopic rod 33 is provided on the upper surface of the sealing plate 15. The telescopic rod 33 is arranged close to the edge of the sealing plate 15. The telescopic rod 33 is used to keep the sealing plate 15 in a floating path in the vertical direction; one end of the telescopic rod 33 is fixedly connected to the sealing plate 15, and the other end of the telescopic rod 33 is arranged on the limit stop block 141.

[0042] In the above embodiment, the telescopic rod 33 is arranged to guide the movement of the sealing plate 15, thereby preventing the sealing plate 15 from lateral deviation during movement and causing uneven force on the inner wall of the second hole 14, thereby affecting the sealing between the two. It can also prevent the limiting groove 151 on the sealing plate 15 and the limiting block 141 from having a matching deviation and failing to form an optimal interlocking state.

[0043] In the above embodiment, since the telescopic rod 33 is disposed between the limiting ring 18 and the sealing plate 15, the telescopic rod 33 limits the movable distance between the limiting ring 18 and the sealing plate 15. In order to solve the above problem, in the preferred embodiment provided by the present invention based on the above embodiment, as Figure 6 As shown, a connecting hole 17 is opened on the limiting ring 18, and a slidable top block 171 and a limiting body 172 arranged above the top block 171 are arranged in the connecting hole 17, and the top block 171 is used to connect the telescopic rod 33; as the telescopic rod 33 is subjected to force, the top block 171 gradually slides upward and stops at the limiting body 172, and the base of the telescopic rod 33 retracts into the connecting hole 17.

[0044] In the above embodiment, the top surface of the floating base column 31 is higher than the top edge of the buoyancy tank 1, so that when the operating platform 3 is fixedly connected to the floating base column 31, the operating platform 3 and the buoyancy tank 1 are in a separated state, thereby preventing the buoyancy tank 1 from transmitting vibration to the operating platform 3.

[0045] In the above embodiment, in order to ensure the volume of the fluid material contained in the sub-cavity 12 , the partition 11 is preferably located at the middle height of the inner cavity of the buoyancy tank 1 .

[0046] In the above embodiment, the rigid connection between the shock absorbing column 21 and the buoyancy tank 1 can easily transmit vibration to the bottom of the buoyancy tank 1, thereby increasing the vibration absorption of the fluid material therein. In order to solve the above problem, Figure 3-4 As shown, in a preferred embodiment provided by the present invention based on the above embodiment, a shock absorbing ring 23 is sleeved on the shock absorbing column 21, and the shock absorbing ring 23 is arranged near the bottom of the shock absorbing column 21. The shock absorbing ring 23 is connected to the upper surface of the substrate platform 2 to reduce the vibration transmitted from the substrate platform 2 to the shock absorbing column 21. In this embodiment, when the shock absorbing column 21 is made of a rigid material to ensure the load-bearing capacity, the shock absorbing ring 23 preferably uses a shock-absorbing material, such as rubber, foam, rubber and metal composite materials, etc., so as to reduce the vibration frequency and intensity on the shock absorbing column 21.

[0047] The above embodiments are only exemplary embodiments of the present application and are not intended to limit the present application. The protection scope of the present application is defined by the claims. Those skilled in the art may make various modifications or equivalent substitutions to the present application within the essence and protection scope of the present application, and such modifications or equivalent substitutions shall also be deemed to fall within the protection scope of the present application.

Claims

1. A separate noise reduction and vibration reduction optical platform for patch clamp experiments, comprising: A buoyancy tank (1) is provided with a partition (11) inside thereof. The partition (11) is arranged along the cross section of the buoyancy tank (1). The partition (11) divides the inner cavity of the buoyancy tank (1) into a sub-cavity (12) located below the partition (11). The sub-cavity (12) is used to be filled with fluid; a first hole (13) and a second hole (14) are formed through the partition (11). A movable sealing plate (15) is provided in the second hole (14). The fluid in the sub-cavity (12) can be pressed into the first hole (13) and the second hole (14) by external pressure; a movable bottom plate (16) is provided at the inner bottom of the buoyancy tank (1), and a seal is formed between the ring side of the movable bottom plate (16) and the inner wall of the buoyancy tank (1); A base plate platform (2), the outer bottom of the buoyancy tank (1) is supported and fixed on the base plate platform (2) by a shock absorbing column (21), a damping spring component (22) is provided on the base plate platform (2) and passes through the bottom of the buoyancy tank (1), the damping spring component (22) supports the movable bottom plate (16) to be separated from the inner bottom of the buoyancy tank (1), and the vibration received by the base plate platform (2) is weakened by the shock absorbing column (21) and then transmitted to the buoyancy tank (1) and absorbed by the fluid in the sub-cavity (12); An operating platform (3), the operating platform (3) being arranged above the buoyancy tank (1) and separated therefrom, a floating base column (31) being fixedly connected below the operating platform (3), a floating plate (32) being fixedly connected below the floating base column (31), the floating plate (32) closing the first hole (13) and being movably arranged therein; The invention is characterized in that a bent rod (161) is fixedly provided on the bottom side of the movable bottom plate (16), one end of the bent rod (161) is hingedly connected to a vertical rod (162), one end of the vertical rod (162) is hingedly connected to a swing arm (163), a support rod (164) is provided on the outer wall of the buoyancy tank (1), the support rod (164) is axially connected to one end of the swing arm (163), and the end of the swing arm (163) is provided with an active tooth (165) that rotates with the swing arm A second support rod (166) is fixedly arranged on the first support rod (164), a driven tooth (167) is arranged on the second support rod (166), a linkage rod (168) is arranged on one side of the driven tooth (167), one end of the linkage rod (168) is fixedly connected to the movable bottom plate (16), and a rack (169) is arranged on the other end of the linkage rod (168), and the active tooth (165) and the rack (169) are respectively meshed with the driven tooth (167).

2. A separate noise reduction and vibration reduction optical platform for patch clamp experiments according to claim 1, characterized in that: The first hole (13) is arranged in the middle of the partition plate (11), and the second hole (14) is arranged on an annular path around the first hole (13).

3. A separate noise reduction and vibration reduction optical platform for patch clamp experiments according to claim 2, characterized in that: The area of ​​the movable bottom plate (16) is S, the area of ​​the first hole (13) is S1, the area of ​​the second hole (14) is S2, and the transmission ratio of the driving tooth (165) and the driven tooth (167) is S2:S, so that the volume change of the fluid in the sub-chamber (12) is consistent with the volume change of the fluid in the second hole (14).

4. A separate noise reduction and vibration reduction optical platform for patch clamp experiments according to claim 3, characterized in that: The end of the swing arm (163) and the driving tooth (165) are key-connected.

5. A separate noise reduction and vibration reduction optical platform for patch clamp experiments according to claim 4, characterized in that: A limit stopper (141) is provided on the inner wall of the second hole (14), and the limit stopper (141) is arranged close to the lower end of the second hole (14); The sealing plate (15) is provided with a limiting notch (151), the limiting notch (151) is located on the lower surface of the sealing plate (15), and the limiting stopper (141) corresponds to the position of the limiting notch (151) so as to be embedded therein; The limiting notches (151) are equidistantly arranged in the circumferential direction at the edge of the sealing plate (15); the number of the limiting blocks (141) and the limiting notches (151) is at least 2, and the number of the limiting notches (151) is not less than the number of the limiting blocks (141).

6. A separate noise reduction and vibration reduction optical platform for patch clamp experiments according to claim 5, characterized in that: A telescopic rod (33) is provided on the upper surface of the sealing plate (15), and the telescopic rod (33) is arranged close to the edge of the sealing plate (15). The guide rod (33) is used to keep the sealing plate (15) in a floating path in the vertical direction; one end of the telescopic rod (33) is fixedly connected to the sealing plate (15), and the other end of the telescopic rod (33) is arranged on the limit stopper (141).

7. A separate noise reduction and vibration reduction optical platform for patch clamp experiments according to claim 6, characterized in that: The limit stopper (141) is provided with a connection hole (17), and a slidable top block (171) and a limit body (172) provided above the top block (171) are provided in the connection hole (17), and the top block (171) is used to connect the telescopic rod (33); as the telescopic rod (33) is subjected to force, the top block (171) gradually slides upward and stops at the limit body (172), and the base of the telescopic rod (33) retracts into the connection hole (17).

8. A separate noise reduction and vibration reduction optical platform for patch clamp experiments according to claim 7, characterized in that: The top surface of the floating base column (31) is higher than the top edge of the buoyancy tank (1), so that the operating platform (3) and the buoyancy tank (1) are in a separated state.

9. A separate noise reduction and vibration reduction optical platform for patch clamp experiments according to claim 8, characterized in that: The partition plate (11) is arranged at a middle height position of the inner cavity of the buoyancy tank (1).

10. A separate noise reduction and vibration reduction optical platform for patch clamp experiments according to claim 9, characterized in that: The shock absorbing column (21) is sleeved with a shock absorbing ring (23), the shock absorbing ring (23) being arranged close to the bottom of the shock absorbing column (21), and the shock absorbing ring (23) being connected to the upper surface of the substrate platform (2) to reduce the vibration transmitted from the substrate platform (2) to the shock absorbing column (21).

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