Application of composition containing alpha-pinene and nano SiO2 in panax notoginseng planting

Abstract

The invention provides application of a composition containing alpha-pinene and nano SiO2 in promoting panax notoginseng growth, inducing resistance, preventing and treating panax notoginseng diseases and / or increasing the notoginsenoside content, and further provides a method for promoting panax notoginseng growth, inducing resistance, preventing and treating panax notoginseng diseases and / or increasing the notoginsenoside content. Research finds that in the composition, alpha-pinene and nano SiO2 in a specific dosage ratio are combined for use, the growth of panax notoginseng can be remarkably promoted, resistance can be induced, panax notoginseng diseases can be prevented and treated, and / or the notoginsenoside content can be increased, and the effect is remarkably superior to that of independent use of alpha-pinene and nano SiO2, which shows that after the alpha-pinene and nano SiO2 are combined for use, the yield of panax notoginseng is increased. The composition has a remarkable synergistic effect and has the advantages of low toxicity, no pollution, environmental friendliness and the like. And a green and environment-friendly combination is provided for the development of the pseudo-ginseng industry.

Description

Technical Field

[0001] The invention belongs to the field of agriculture and relates to a method comprising α-pinene and nano-SiO 2 The present invention relates to an application of a composition in Panax notoginseng planting, and specifically relates to an application of the composition in promoting Panax notoginseng growth, inducing resistance, preventing and controlling Panax notoginseng diseases and / or increasing the content of Panax notoginseng saponins; the present invention also relates to a method for promoting Panax notoginseng growth, inducing resistance, preventing and controlling Panax notoginseng diseases and / or increasing the content of Panax notoginseng saponins. Background Art

[0002] Panax notoginseng (Burk.) FHChen is a perennial herbaceous plant of the genus Panax in the Araliaceae family. It is a precious Chinese medicinal material unique to China and one of the earliest plants with medicinal and edible properties in my country. It is named Panax notoginseng because it is dug three to seven years after sowing and each plant has three petioles, each with seven leaves. Panax notoginseng has the effects of dispersing blood stasis and stopping bleeding, reducing swelling and relieving pain, and has significant therapeutic effects on the treatment of cardiovascular and cerebrovascular diseases. Yunnan is the main production area and origin of Panax notoginseng, but due to its growth habits, it is prone to serious diseases such as Panax notoginseng root rot and black spot disease. In addition, in recent years, the pursuit of high-yield cultivation mode of large fertilizers, large water and large yields in farmland has led to problems such as reduced efficacy, excessive pesticide and heavy metal residues, and continuous cropping obstacles in the Panax notoginseng industry, which seriously affects the yield and quality of Panax notoginseng and restricts the sustainable development of the Panax notoginseng industry.

[0003] The ecological planting model of Panax notoginseng under pine forests takes advantage of the rich biodiversity of Yunnan to build an environment conducive to the growth of Panax notoginseng. In this model, the various allelopathic substances of pine trees not only promote the healthy growth of Panax notoginseng, but also stabilize the ecological environment under the forest. The volatile α-pinene is the most abundant allelopathic substance in pine trees. α-pinene is a terpene organic compound that can be obtained from pine trees and other plants. Its chemical formula is C 10 H 16 , molecular weight is 136.234, it is a colorless transparent liquid, volatile, and low in water solubility. α-pinene is an important raw material for synthetic fragrances, and can also be used to flavor daily chemicals and other industrial products, and can also be used as a synthetic lubricant and plasticizer.

[0004] At present, α-pinene has been proven to promote the growth of Panax notoginseng by directly inducing the basic metabolism, photosynthesis and plant hormone conduction of Panax notoginseng, and to induce the upregulation of Panax notoginseng resistance proteins and key genes of resistance pathways to improve its disease resistance. Patent CN113892484A discloses an application of α-pinene in promoting the growth of Panax notoginseng and inducing resistance. By fumigating Panax notoginseng seeds or plants with α-pinene in a confined space, the effect of α-pinene on the germination of Panax notoginseng seeds and inducing Panax notoginseng to produce disease resistance was evaluated. However, due to the characteristics of α-pinene being volatile and insoluble in water, the application of α-pinene in the field will greatly reduce the effective utilization rate of α-pinene due to the inability to provide confined conditions. Therefore, the methods of the prior art cannot ensure the feasibility of α-pinene in production applications.

[0005] Nano-SiO 2 Commonly known as white carbon black, it is an amorphous white powder with the advantages of small particle size (1-100nm), large specific surface area, and strong adsorption capacity. However, so far, no α-pinene and nano-SiO 2 There are reports on combined use in promoting Panax notoginseng growth, inducing resistance, preventing and controlling Panax notoginseng diseases and / or increasing Panax notoginseng saponin content. Summary of the invention

[0006] In order to solve the shortcomings of the prior art, the present invention provides a method comprising α-pinene and nano-SiO 2 The invention relates to the use of the composition in promoting the growth of Panax notoginseng, inducing resistance, preventing and controlling Panax notoginseng diseases and / or increasing the content of Panax notoginseng saponins, and related methods.

[0007] The present invention finds that α-pinene and nano-SiO 2 The combined use can significantly promote the growth of Panax notoginseng, induce resistance, prevent and control Panax notoginseng diseases and / or increase the content of Panax notoginseng saponins, solving the problem of limited use of α-pinene, an active substance, due to its low effective utilization rate in practical applications, and provides a green and environmentally friendly combined combination for the development of the Panax notoginseng industry.

[0008] Specifically, the present invention is realized through the following technical solutions:

[0009] In a first aspect, the present invention provides a composition for promoting growth of Panax notoginseng, inducing resistance, preventing and controlling Panax notoginseng diseases and / or increasing the content of Panax notoginseng saponins, wherein the composition comprises α-pinene and nano-SiO 2 .

[0010] In a preferred embodiment, the composition is composed of α-pinene and nano-SiO 2 composition.

[0011] In a preferred embodiment, the application comprises treating Panax notoginseng with the composition, including foliar spraying of Panax notoginseng plants with the composition.

[0012] In a preferred embodiment, the α-pinene and the nano-SiO 2 The volume-to-weight ratio (μL / mg) is 1:1-15, preferably 1:1-10.

[0013] In a preferred embodiment, the promoting the growth of Panax notoginseng includes promoting the biomass accumulation and root growth of Panax notoginseng, improving the quality of Panax notoginseng, and increasing the yield of Panax notoginseng.

[0014] In a preferred embodiment, the induced resistance includes inducing Panax notoginseng to produce disease resistance, and the disease resistance includes resistance to Panax notoginseng pathogens.

[0015] Preferably, the disease resistance includes resistance to Panax notoginseng black spot pathogen.

[0016] In a preferred embodiment, the control of Panax notoginseng diseases includes control of Panax notoginseng root rot and black spot disease.

[0017] In a preferred embodiment, increasing the notoginsenoside content includes increasing the notoginsenoside total content.

[0018] In the present invention, notoginseng total saponins are the main active ingredients of notoginseng, which have the effects of reducing swelling and pain, promoting blood circulation and removing blood stasis, etc., mainly including notoginseng saponins R 1 , Ginsenoside R g1 , Ginsenoside Re, Ginsenoside R b1 , Ginsenoside R d .

[0019] In a preferred embodiment, the composition can be prepared into an agriculturally acceptable formulation with auxiliary materials when used, and the formulation includes a spray, an aqueous solvent, and the like.

[0020] In a second aspect, the present invention provides a method for promoting the growth of Panax notoginseng, inducing resistance, preventing and controlling Panax notoginseng diseases and / or increasing the content of Panax notoginseng saponins, the method comprising treating Panax notoginseng with the composition described in the first aspect above.

[0021] In a preferred embodiment, the treatment comprises foliar spraying of Panax notoginseng plants with the composition.

[0022] In a preferred embodiment, the α-pinene and the nano-SiO 2 The volume-to-weight ratio (μL / mg) is 1:1-15, preferably 1:1-10.

[0023] In a preferred embodiment, the promoting the growth of Panax notoginseng includes promoting the biomass accumulation and root growth of Panax notoginseng, improving the quality of Panax notoginseng, and increasing the yield of Panax notoginseng.

[0024] In a preferred embodiment, the induced resistance includes inducing Panax notoginseng to produce disease resistance, and the disease resistance includes resistance to Panax notoginseng pathogens.

[0025] Preferably, the disease resistance includes resistance to Panax notoginseng black spot pathogen.

[0026] In a preferred embodiment, the control of Panax notoginseng diseases includes control of Panax notoginseng root rot and black spot disease.

[0027] In a preferred embodiment, increasing the notoginsenoside content includes increasing the notoginsenoside total content.

[0028] In a preferred embodiment, the composition can be prepared into an agriculturally acceptable formulation with auxiliary materials when used, and the formulation includes a spray, an aqueous solvent, and the like.

[0029] In a preferred embodiment, the present invention provides a Panax notoginseng growth promoter, a Panax notoginseng inducer, a Panax notoginseng disease control agent, and a Panax notoginseng saponin content enhancer, which contain the composition.

[0030] Preferably, the Panax notoginseng growth promoter, Panax notoginseng inducer, Panax notoginseng disease control agent and Panax notoginseng saponin content enhancer of the present invention also include auxiliary materials so as to be made into agriculturally acceptable dosage forms, and the dosage forms include sprays, aqueous solvents and the like.

[0031] Preferably, the composition is used in the form of an aqueous solution.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] The invention discloses a novel nanostructured carbon foam comprising α-pinene and nano-SiO 2 The application of the composition in promoting the growth of Panax notoginseng, inducing resistance, preventing and controlling Panax notoginseng diseases and / or increasing the content of Panax notoginseng saponins. Experiments have shown that the specific dosage ratio of α-pinene and nano-SiO 2 The combined use of these two ingredients can significantly promote the growth of Panax notoginseng, induce resistance, prevent and treat Panax notoginseng diseases and / or increase the content of Panax notoginseng saponins, and the effect is significantly better than that of α-pinene and nano-SiO 2 This indicates that the combination of α-pinene with nano-SiO 2 After combined use, it has significant synergistic effect, and has the advantages of low toxicity, no pollution, and environmental friendliness, etc. It provides a green and environmentally friendly combination for the development of the Panax notoginseng industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0035] Figure 1 α-pinene and nano-SiO 2 Diagram showing the determination of lesion area of ​​Panax notoginseng induced by combined use to resist black spot disease.

[0036] Figure 2 α-pinene and nano-SiO 2 Effect of combined use on chlorophyll content of Panax notoginseng.

[0037] Figure 3 α-pinene and nano-SiO 2 Effects of combined use on the growth of Panax notoginseng, (A) represents the fresh weight of the aboveground part, and (B) represents the dry weight of the underground part.

[0038] Figure 4 α-pinene and nano-SiO 2 Effect of combined use on the incidence of Panax notoginseng diseases, (A) α-pinene and nano-SiO 2 Effect of combined use on the incidence of Panax notoginseng root rot; (B) α-pinene and nano-SiO 2 The effect of combined use on the incidence of Panax notoginseng black spot disease.

[0039] Figure 5 α-pinene and nano-SiO 2 Effect of combined use on the content of total saponins in Panax notoginseng. DETAILED DESCRIPTION

[0040] The embodiments of the present invention are described in detail below. The embodiments are given to better illustrate the contents of the present invention and are only used to explain the present invention, but should not be understood as limiting the present invention.

[0041] If no specific techniques or conditions are specified in the examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0042] Example 1: α-pinene and nano-SiO 2 Experiment on inducing disease resistance of Panax notoginseng by combined use

[0043] First, select the Panax notoginseng potted plants that have grown evenly after transplanting and remove the weeds on the soil surface. After watering thoroughly, leave them for 1 day. Then, use a fresh-keeping bag to cover the Panax notoginseng potted plants from the bottom so that the above-ground part of the Panax notoginseng is exposed outside. Use tape to seal the gaps in the fresh-keeping bag to prevent the sprayed substances from affecting the soil. Put the 6 treated Panax notoginseng potted plants into a transparent plastic incubator and incubate them with water, 10 mg / L SiO2 Aqueous solution, 100mg / L SiO 2 Aqueous solution, 10μL / L α-pinene aqueous solution, 10mg / LSiO 2 +10μL / L α-pinene aqueous solution, 100mg / LSiO 2 The surface of Panax notoginseng stems and leaves was sprayed with 10 μL / L α-pinene aqueous solution. After closed induction for 1 day, the leaf cake of Panax notoginseng black spot pathogen SL17 was inoculated on the leaf surface. The leaves were moisturized and cultured in a transparent plastic incubator for about 5 days. After the disease occurred, the diseased leaves were collected and the lesions were scanned. The relative area of ​​the lesions was used to evaluate the interaction between α-pinene and nano-SiO 2 The combined use of Panax notoginseng to induce disease resistance, the results are as follows Figure 1 As shown (*P<0.05, **P<0.01, ***P<0.001).

[0044] from Figure 1 It can be seen that α-pinene and nano-SiO 2 The combined use can significantly reduce the incidence area of ​​Panax notoginseng black spot disease, indicating that α-pinene and nano-SiO 2 The combined use significantly improved the ability of inducing Panax notoginseng to resist black spot disease.

[0045] The results showed that 10 and 100 mg / L nano-SiO 2 When used in combination with α-pinene, the ability of α-pinene to induce Panax notoginseng to resist black spot disease was enhanced.

[0046] Example 2: α-pinene and nano-SiO 2 Experiment on improving the chlorophyll content of Panax notoginseng by combined use

[0047] A Panax notoginseng experimental plot (under forest) was set up in Lancang County, Pu'er City for the experiment (experimental plot: area 1.46m 2 , plant density is about 82 plants / m 2 , 3 rows of plants were set as isolation belts), and the experimental plots were randomly distributed. 2 Aqueous solution, 10μL / L α-pinene aqueous solution, 10mg / L SiO 2 +10μL / L α-pinene aqueous solution was sprayed on the surface of Panax notoginseng stems and leaves, once every 15 days, for a total of 8 times (July 15, 2022 to October 31, 2022), and each treatment was repeated 5 times. 15 days after the last spraying (November 15, 2022), a handheld chlorophyll measuring instrument was used to directly measure the chlorophyll content of Panax notoginseng at 20 points in each test plot. The results are as follows Figure 2 As shown (*P<0.05, **P<0.01).

[0048] from Figure 2 It can be seen that α-pinene and nano-SiO2 The combined use significantly increased the chlorophyll content of Panax notoginseng, which was comparable to the use of nano-SiO 2 Compared with α-pinene and α-pinene, the chlorophyll contents increased by 8.7% and 5.84% respectively.

[0049] Example 3: α-pinene and nano-SiO 2 Experiment on promoting the growth of Panax notoginseng by combined use

[0050] A Panax notoginseng experimental plot (under forest) was set up in Lancang County, Pu'er City for the experiment (experimental plot: area 1.46m 2 , plant density is about 82 plants / m 2 , set 3 rows of plants as isolation belts), and the experimental plots were randomly distributed. 2 Aqueous solution, 10μL / L α-pinene aqueous solution, 100mg / L SiO 2 +10μL / L α-pinene aqueous solution was sprayed on the surface of Panax notoginseng stems and leaves, once every 15 days, for a total of 8 times (July 15, 2022 to October 31, 2022), and each treatment was repeated 5 times. 15 days after the last spraying (November 15, 2022), 20 Panax notoginseng plants were randomly selected in each test plot using the five-point sampling method to determine the weight of fresh matter and dry matter in the aboveground and underground parts. The results are as follows Figure 3 As shown (*P<0.05, **P<0.01, ***P<0.001).

[0051] Fresh weight: Wash the dust on the surface of the Panax notoginseng plant with clean water and wipe it dry with absorbent paper, weigh the weight of the leaves and stems of the aboveground part of the Panax notoginseng plant, and wash the soil on the root system of the Panax notoginseng plant with clean water and wipe it dry with absorbent paper, and weigh the weight of the taproot and fibrous roots of the underground part of the Panax notoginseng plant, and the fresh weight of the underground part of the Panax notoginseng plant.

[0052] Dry weight: Put the cleaned Panax notoginseng tissue into an envelope and mark it. Put it into an oven and first kill it at 105℃ for 5 minutes, then adjust the temperature to 50℃ and dry it to constant weight before weighing. The weight of the above-ground part of the Panax notoginseng plant after drying is the above-ground dry weight, and the weight of the underground part after drying is the underground dry weight.

[0053] from Figure 3 It can be seen that α-pinene and nano-SiO 2 The combined use significantly promoted the accumulation of fresh matter in the aboveground part of Panax notoginseng and the dry weight of the underground part of Panax notoginseng, indicating that α-pinene and nano-SiO 2 Combined use can significantly promote the growth of Panax notoginseng.

[0054] Example 4: α-pinene and nano-SiO 2 Experiment on combined use to control Panax notoginseng diseases

[0055] A Panax notoginseng experimental plot (under forest) was set up in Lancang County, Pu'er City for the experiment (experimental plot: area 1.46m 2 , plant density is about 82 plants / m 2 , 3 rows of plants were set as isolation belts), and the experimental plots were randomly distributed. 2 Aqueous solution, 100mg / L SiO 2 Aqueous solution, 10μL / L α-pinene aqueous solution, 50mg / L SiO 2 +10μL / L α-pinene aqueous solution, 100mg / L SiO 2 +10μL / L α-pinene aqueous solution was sprayed on the surface of Panax notoginseng stems and leaves, once every 15 days, for a total of 8 times (July 15, 2022 to October 31, 2022), and each treatment was repeated 5 times. 15 days after the last spraying (November 15, 2022), the disease status of Panax notoginseng plants in the test plot was counted. The results are as follows Figure 4 As shown (*P<0.05, **P<0.01, ***P<0.001).

[0056] Incidence rate = number of diseased plants / total number of plants*100%

[0057] from Figure 4 It can be seen that α-pinene and nano-SiO 2 The combined use significantly reduced the incidence of Panax notoginseng root rot and black spot, indicating that α-pinene and nano-SiO 2 Combined use can significantly improve the prevention and control effect of Panax notoginseng root rot and black spot disease.

[0058] Example 5: α-pinene and nano-SiO 2 Experiment on increasing the content of notoginseng saponin by combined use

[0059] A Panax notoginseng experimental plot (under forest) was set up in Lancang County, Pu'er City for the experiment (experimental plot: area 1.46m 2 , plant density is about 82 plants / m 2 , set 3 rows of plants as isolation belts), and the experimental plots were randomly distributed. 2 Aqueous solution, 10μL / L α-pinene aqueous solution, 100mg / L SiO 2 +10μL / Lα-pinene aqueous solution was sprayed on the surface of Panax notoginseng stems and leaves, once every 15 days, for a total of 8 times (July 15, 2022 to October 31, 2022), and each treatment was repeated 5 times. 15 days after the last spraying (November 15, 2022), 20 plants were randomly selected from each test plot using the five-point sampling method to test the quality of Panax notoginseng saponins and compare the total saponins in the underground part (R1 +R e +R g1 +R d +R b1 ) content.

[0060] Weigh 0.2 g of dried, ground, and sieved (100 mesh) sample and put it into a 50 mL centrifuge tube. Add 15 mL of 75% methanol solution (75% methanol: 25% distilled water) into the centrifuge tube. Oscillate and ultrasonicate for 30 min. After the ultrasonication, let it stand for about 30 min. Centrifuge at 14000 rpm for 15 min. Take a small amount of supernatant to rinse the GC injection bottle. Finally, take 1 mL of supernatant and add it to the injection bottle for use.

[0061] The content of saponin was determined by Waters ACQUITY UPLC (column: ACQUITY UPLC @ BEH-C18 (1.7um × 2.1 × 100mm), mobile phase A and B were acetonitrile and water, flow rate was 0.25mL / min, injection volume was 2μL, gradient elution (0-3min, 19.5% A; 3-10min, 19.5% A-49.5% A; 10-11min, 49.5% A-95% A; 11-13min, 95% A; column temperature 30°C, collection wavelength range 190-400nm, resolution 1.2nm, sampling rate 10pt / s). The results are shown in Figure 2. Figure 5 As shown (*P<0.05, **P<0.01, ***P<0.001).

[0062] from Figure 5 It can be seen that α-pinene and nano-SiO 2 The combined use significantly increased the total saponin content in the underground part of Panax notoginseng.

[0063] In summary, in the cultivation of Panax notoginseng, α-pinene and nano-SiO 2 The combined use of α-pinene and nano-SiO2 can significantly promote the growth of Panax notoginseng, induce disease resistance, prevent and treat Panax notoginseng diseases, and increase the content of Panax notoginseng saponins, and the effect is significantly better than that of α-pinene and nano-SiO2. 2 This indicates that the combination of α-pinene with nano-SiO 2 When used in combination, they have a significant synergistic effect.

[0064] Obviously, the above embodiments are only examples for clearly explaining the present invention, and are not intended to limit the implementation methods of the present invention. Those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A composition for use in promoting growth of Panax notoginseng, inducing resistance, preventing and treating Panax notoginseng diseases and / or increasing the content of Panax notoginseng saponins, characterized in that: The composition comprises α-pinene and nano-SiO2.

2. A method for promoting the growth of Panax notoginseng, inducing resistance, preventing and controlling Panax notoginseng diseases and / or increasing the content of Panax notoginseng saponins, characterized in that: The method comprises treating Panax notoginseng with the composition described in claim 1.

3. The use or method according to claim 1 or 2, characterized in that: The volume weight ratio (μL / mg) of the α-pinene to the nano-SiO2 is 1:1-15, preferably 1:1-10.

4. The use or method according to claim 1 or 2, characterized in that: The promoting of Panax notoginseng growth includes promoting the biomass accumulation and root growth of Panax notoginseng, improving the quality of Panax notoginseng, and increasing the yield of Panax notoginseng.

5. The use or method according to claim 1 or 2, characterized in that: The induced resistance includes inducing Panax notoginseng to produce disease resistance, and the disease resistance includes resistance to Panax notoginseng pathogens; preferably, the disease resistance includes resistance to Panax notoginseng black spot pathogen.

6. The use or method according to claim 1 or 2, characterized in that: The Panax notoginseng disease prevention and control includes the prevention and control of Panax notoginseng root rot and black spot disease.

7. The use or method according to claim 1 or 2, characterized in that: The increasing of notoginsenoside content includes increasing the notoginsenoside total content.

8. The use or method according to claim 1 or 2, characterized in that: The application or method comprises spraying α-pinene and nano-SiO2 on the leaves of Panax notoginseng.

9. The use or method according to claim 1 or 2, characterized in that: When used, the composition is prepared into an agriculturally acceptable dosage form with auxiliary materials, and the dosage form includes a spray and a water solvent.

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