Traditional Chinese medicine composition for treating liver injury as well as preparation method and application thereof

Abstract

The invention discloses a traditional Chinese medicine composition for treating liver injury and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition comprises astragalus membranaceus, angelica sinensis, radix ophiopogonis, vinegar schisandra chinensis, fructus lycii, chrysanthemum and codonopsis pilosula. The traditional Chinese medicine composition disclosed by the invention can be used for treating liver injury caused by various reasons through the synergistic effect of all the components. Animal experiments prove that the traditional Chinese medicine composition can remarkably improve liver function indexes and liver histopathologic injury of animals with liver injury caused by medicines such as cyclophosphamide and the like, can regulate blood indexes and related factor levels, is high in safety and has a wide application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and in particular to a traditional Chinese medicine composition for treating liver damage and application thereof. Background Art

[0002] The liver is an extremely important organ in the human body and plays a key role in many physiological processes such as metabolism, detoxification, and synthesis. Liver injury, especially drug-induced liver injury (DILI), is the most common cause of acute liver failure in Western countries. A large number of commonly used drugs, especially chemotherapy drugs, can cause liver injury. DILI is usually divided into three types: direct dose-dependent liver injury, specific liver injury, and indirect liver injury. Direct dose-dependent liver injury is caused by drugs or substances that are toxic to the liver.

[0003] Cyclophosphamide (CTX) is a nitrogen mustard derivative that is hydrolyzed by excessive phosphamidase or phosphatase in the liver or tumors after entering the human body, and becomes activated phosphoramide nitrogen mustard. It has a broad anti-tumor spectrum and is the first so-called "latent" broad-spectrum anti-tumor drug. It is effective against leukemia and solid tumors. However, cyclophosphamide has certain adverse reactions such as bone marrow suppression and liver and kidney damage in clinical practice.

[0004] Therefore, it is urgent to develop a safe, effective and widely applicable drug for the treatment of liver damage such as cyclophosphamide. Traditional Chinese medicine has a long history and rich experience in the treatment of liver diseases. It is of great significance to explore effective drugs for the treatment of liver damage from traditional Chinese medicine. Summary of the invention

[0005] Purpose of the invention: The purpose of the present invention is to provide a safe, effective and widely applicable Chinese medicine composition for treating liver damage. Another purpose of the present invention is to provide a preparation method and application of the above Chinese medicine composition.

[0006] Technical solution: In order to achieve the above purpose, the technical solution adopted by the present invention is:

[0007] The invention provides a traditional Chinese medicine composition for treating liver damage. The composition comprises astragalus, angelica, ophiopogon, schisandra chinensis, wolfberry, chrysanthemum and codonopsis pilosula.

[0008] As a preferred embodiment, the above-mentioned traditional Chinese medicine composition for treating liver damage comprises the following traditional Chinese medicine raw materials in parts by weight: 10-30 parts of astragalus, 3-6 parts of angelica, 5-15 parts of ophiopogon, 2-8 parts of schisandra chinensis, 5-15 parts of wolfberry, 5-15 parts of chrysanthemum, and 5-15 parts of codonopsis.

[0009] As a preferred embodiment, the above-mentioned traditional Chinese medicine composition for treating liver damage comprises the following traditional Chinese medicine raw materials in parts by weight: 15-25 parts of astragalus, 4-6 parts of angelica, 8-12 parts of ophiopogon, 4-7 parts of schisandra chinensis, 8-12 parts of wolfberry, 8-12 parts of chrysanthemum, and 8-12 parts of codonopsis.

[0010] As a further preferred embodiment, the weight ratio of the above ingredients is: 20 parts of Astragalus, 4 parts of Angelica, 10 parts of Ophiopogon, 5 parts of Schisandrae Chinensis, 10 parts of Lycium barbarum, 10 parts of Chrysanthemum, and 10 parts of Codonopsis.

[0011] A method for preparing a Chinese medicine composition for treating liver damage comprises the following steps:

[0012] Step 1: Soak astragalus, angelica, ophiopogon, vinegar schisandra, wolfberry, chrysanthemum and codonopsis in water, reflux extraction, filter, combine the extracts, and obtain liquid A;

[0013] Step 2: Add water to the residue after filtering the above medicinal liquid, decoct and extract, filter, and combine the filtrate to obtain medicinal liquid B;

[0014] Step 3: Mix the medicinal solution A and the medicinal solution B, and concentrate them to obtain a decoction.

[0015] As a preferred embodiment, the method for preparing the above-mentioned Chinese medicine composition for treating liver injury comprises the following steps:

[0016] Step 1: add 6 to 12 times the amount of water according to the mass volume ratio to Astragalus, Angelica, Ophiopogon, Schisandrae Chrysanthemum, Lycium barbarum, Chrysanthemum and Codonopsis, soak for 0.5 to 3 hours, reflux extraction for 1 to 2 hours, extract continuously for 2 to 3 times, filter the extract, separate the supernatant from the residual drug residue, and obtain drug solution A;

[0017] Step 2: Add 8 to 10 times the amount of water to the residue after filtering the medicinal liquid according to the mass volume ratio, and boil and extract for 1 to 2 hours to obtain medicinal liquid B;

[0018] Step 3: Mix the drug solution A and the drug solution B, and concentrate them to obtain an extract.

[0019] A Chinese medicine preparation of a Chinese medicine composition for treating liver damage is prepared by mixing a Chinese medicine extract with a pharmaceutically acceptable carrier into tablets, granules, capsules, pills, powders, pastes or oral liquids.

[0020] The decoction preparation method is:

[0021] Step 1: Soak Astragalus, Angelica, Ophiopogon, Schisandrae Chrysanthemum, Lycium barbarum, Chrysanthemum, and Codonopsis in water (1:10, w / v) for 3 hours, reflux extraction for 1.5 hours, and extract twice continuously. Filter the extract with 3 layers of gauze, separate the supernatant from the residual drug residue, and obtain drug solution A.

[0022] Step 2: Add water (1:8, w / v) to the residue after filtering the above medicinal liquid and boil for 1.5 hours to obtain medicinal liquid B.

[0023] Step 3: Mix the medicinal solution A and the medicinal solution B, and continue to boil and concentrate to obtain a decoction.

[0024] The granule preparation method is:

[0025] Step 1:

[0026] First, prepare the Chinese medicine liquid according to the decoction preparation method, dry the drug extract at 80°C under reduced pressure to obtain dry powder, then add appropriate amount of microcrystalline cellulose, dextrin and other auxiliary materials according to the following ratio (composition extract dry powder: dextrin: microcrystalline cellulose = 10:7:3) and mix evenly, add a certain amount of wetting agent (distilled water) to make a soft material, pass through a No. 2 sieve (16 mesh) to granulate, and dry at 60°C to constant weight, that is, the moisture content is 3%-5%. Select the particles that pass through the No. 1 sieve and cannot pass through the No. 5 sieve, use a suitable granule filling machine, and fill the dried particles into packaging bags, with a filling amount of 5-10 grams per bag.

[0027] The preparation method of capsules is as follows:

[0028] Step 1:

[0029] First, prepare the Chinese medicine liquid according to the decoction preparation method, dry the drug extract at 80°C under reduced pressure to obtain dry powder, then add appropriate amount of microcrystalline cellulose, dextrin and other auxiliary materials according to the following ratio (composition extract dry powder: dextrin: microcrystalline cellulose = 10:7:3) and mix evenly, add a certain amount of wetting agent (distilled water) to make a soft material, pass through a No. 2 sieve (16 mesh) to granulate, and dry at 60°C to constant weight, that is, the moisture content meets the requirements, usually 3%-5%. Select the particles that pass through the No. 1 sieve and cannot pass through the No. 5 sieve, use a suitable capsule filling machine, and fill the dried particles into capsules, with each capsule filling amount of 0.3-0.5g.

[0030] The prescription of the Chinese medicine composition of the present invention is based on:

[0031] Angelica: It has the effects of nourishing blood, activating blood circulation, regulating menstruation and relieving pain, and moistening the intestines and relieving constipation. In this composition, Angelica can improve the blood circulation in the liver and promote the nutrient supply of liver cells, thereby helping to repair and regenerate damaged liver cells. The various active ingredients it contains, such as ferulic acid, have antioxidant and anti-inflammatory effects, can reduce liver inflammatory reactions, and protect liver cells from free radical damage.

[0032] Lycium barbarum: nourishes the liver and kidneys, improves the quality of life and improves eyesight. Lycium barbarum is rich in vitamins, minerals and Lycium barbarum polysaccharides. Lycium barbarum polysaccharides have the functions of regulating immune function, anti-oxidation, lowering blood lipids, etc., can enhance the body's immunity, inhibit hepatocyte lipid peroxidation, protect against liver damage caused by cyclophosphamide, and help improve the liver's metabolic function.

[0033] Chrysanthemum: dispel wind and clear heat, soothe the liver and improve eyesight, clear heat and detoxify. The flavonoids in chrysanthemum have antioxidant, anti-inflammatory, antibacterial and other activities. In the present invention, chrysanthemum may reduce liver inflammation through its anti-inflammatory effect, while its antioxidant properties help to remove free radicals in the liver, reduce the damage of oxidative stress to the liver, and maintain the normal structure and function of liver cells.

[0034] Schisandra chinensis: astringent, invigorating Qi and promoting the production of body fluid, nourishing the kidney and calming the mind. Schisandra chinensis has a protective effect on the liver, promoting the synthesis of liver cell protein and glycogen, reducing liver cell damage, and enhancing the liver's detoxification function. Its ingredients such as schisandrin B have antioxidant and anti-liver fibrosis effects, can inhibit the activation of liver stellate cells, reduce the synthesis of collagen, and thus delay the process of liver fibrosis.

[0035] Codonopsis: Strengthens the spleen and lungs, nourishes blood and produces body fluids. Codonopsis can regulate the body's immune function and enhance the body's resistance. In liver diseases, immune dysfunction is often closely related to the occurrence and development of liver damage. Codonopsis may help reduce liver immune damage and promote liver self-repair by regulating immune balance. At the same time, Codonopsis may also provide sufficient energy support for the repair of liver cells by improving liver energy metabolism.

[0036] Astragalus: replenishes qi and raises yang, consolidates the exterior and stops sweating, promotes diuresis and reduces swelling, promotes the production of body fluids and nourishes blood, relieves stagnation and relieves arthritis, removes toxins and discharges pus, and heals sores and promotes tissue regeneration. Astragalus has multiple pharmacological effects, such as enhancing the body's immune function, anti-oxidation, anti-inflammatory, and anti-liver fibrosis. Astragalus polysaccharides and other ingredients in Astragalus can promote liver cell proliferation, inhibit liver cell apoptosis, improve the liver's antioxidant capacity, reduce inflammatory reactions, and have a significant protective effect on liver damage. In addition, Astragalus can also regulate liver microcirculation, improve liver blood perfusion, and facilitate liver cell regeneration and repair.

[0037] Ophiopogon japonicus: nourishes yin and produces body fluid, moistens the lungs and clears the mind. Ophiopogon japonicus can nourish stomach yin and promote the absorption of nutrients by the gastrointestinal tract, thereby providing sufficient nutrients for the liver and facilitating liver repair. The ingredients contained in it, such as ophiopogon saponins, have antioxidant and anti-inflammatory effects, can reduce liver inflammatory reactions, protect liver cells from damage, and may have a certain inhibitory effect on the process of liver fibrosis.

[0038] The components of the Chinese medicine composition of the present invention are compatible with each other and work synergistically. The main drugs of angelica, codonopsis and astragalus are combined with lycium barbarum and ophiopogon japonicus as auxiliary drugs, which are yin-nourishing drugs, to replenish qi and blood, nourish yin and nourish the liver, so that the liver is fully nourished; the combination of chrysanthemum, schisandra chinensis and other heat-clearing and liver-calming, astringent and astringent drugs with the above drugs can not only clear the heat evil in the liver, but also astringe liver qi and prevent liver yang from rising, and play a role in protecting the liver, promoting liver repair and regulating liver function, thereby effectively treating liver damage.

[0039] Beneficial effects: The present invention adopts the theory of dialectical treatment based on traditional Chinese medicine theory, and optimizes the composition of traditional Chinese medicine raw materials. The traditional Chinese medicine composition obtained by the present invention has a significant effect of treating liver damage, and can be used to treat liver damage caused by various reasons, including but not limited to drug-induced liver damage, chemical liver damage, alcoholic liver damage, viral hepatitis, etc. In particular, it has a significant effect of preventing and treating liver damage caused by cyclophosphamide. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 Effect of the composition on body weight of mice with liver injury model.

[0041] Figure 2 Effect of the composition on the liver organ coefficient of liver injury model mice.

[0042] Figure 3 Effect of the composition on blood routine test in mice with liver injury model.

[0043] Figure 4 Effect of the composition on liver pathology in liver injury model mice.

[0044] Figure 5 Effects of the composition on blood biochemistry of liver injury model mice (K represents blank group, M represents model group, Y represents positive control group, G represents high-dose group, Z represents medium-dose group, and D represents low-dose group). DETAILED DESCRIPTION

[0045] The present invention is further explained below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, various equivalent modifications of the present invention by those skilled in the art all fall within the scope defined by the claims attached to this application.

[0046] Example 1: A method for preparing a Chinese medicine composition for treating liver damage, comprising the following steps:

[0047] Step 1: Take 150g of Astragalus, 30g of Angelica, 75g of Ophiopogon, 37.5g of Schisandrae Chinensis, 75g of Lycium barbarum, 75g of Chrysanthemum, and 75g of Codonopsis, a total of 517.5g, soak in water (1:10, w / v) for 3 hours, reflux extraction for 1.5h, and extract twice continuously. Filter the extract with 3 layers of gauze, separate the supernatant and the residue, and combine the filtrate;

[0048] Step 2: Add water (1:8, w / v) to the residue after filtering the above medicinal liquid and boil for 1.5 hours to obtain medicinal liquid B.

[0049] Step 3: Mix the medicinal solution A and the medicinal solution B, and concentrate them to obtain a Chinese herbal medicine extract.

[0050] Example 2: Granule Preparation

[0051] Same as the steps in Example 1, 60 g of Astragalus, 12 g of Angelica, 30 g of Ophiopogon, 15 g of Schisandrae Chinensis, 30 g of Lycium barbarum, 30 g of Chrysanthemum, and 30 g of Codonopsis were extracted with water, and the extract was concentrated to 1 g·mL -1 Density. Dry the drug extract powder under reduced pressure at 80°C, then weigh 10g of the combined extract powder, 7g of dextrin, and 3g of microcrystalline cellulose according to the following ratio (combination extract powder: dextrin: microcrystalline cellulose = 10:7:3), add a certain amount of wetting agent (distilled water) to make a soft material, granulate through a No. 2 sieve (16 mesh), and dry at 60°C to constant weight. Select the granules that pass through the No. 1 sieve and cannot pass through the No. 5 sieve, use a suitable granule filling machine, and fill the dried granules into packaging bags, with a filling amount of 5-10g per bag.

[0052] Example 3: Capsule Preparation

[0053] Same as the steps in Example 1, 60 g of Astragalus, 12 g of Angelica, 30 g of Ophiopogon, 15 g of Schisandrae Chinensis, 30 g of Lycium barbarum, 30 g of Chrysanthemum, and 30 g of Codonopsis were extracted with water, and the extract was concentrated to 1 g·mL -1 Density. Dry the drug extract powder under reduced pressure at 80°C, then weigh 10g of the combined extract powder, 7g of dextrin, and 3g of microcrystalline cellulose according to the following ratio (combination extract powder: dextrin: microcrystalline cellulose = 10:7:3), add a certain amount of wetting agent (distilled water) to make a soft material, pass through a No. 2 sieve (16 mesh) to granulate, and dry at 60°C to constant weight. Select the particles that pass through the No. 1 sieve and cannot pass through the No. 5 sieve, use a capsule filling machine, and fill the dried particles into the capsule shell, with each capsule filling amount of 0.3-0.5 grams.

[0054] Example 4: Experimental study on improving liver damage

[0055] Take 36 healthy BALB / C mice and randomly divide them into 6 groups. Give the Chinese medicine composition extract prepared in Example 1. After 3 days, high, medium and low dose groups (9g / kg, 4.5g / kg, 2.25g / kg) were modeled (continued to be administered on the same day). The model and each administration group were injected with CTX (cyclophosphamide) by tail vein, 40mg / kg CTX for 3 days, and the blank group was injected with an equal amount of normal saline. On the first day of modeling, the positive group was gavaged with 2.0g / kg of Qizao granules, and after 12 days of oral gavage, various indicators were measured.

[0056] Animal weight changes: The animals were weighed every day. On the 12th day, the mice were weighed and killed by cervical dislocation. The livers were taken out and the fascia was removed. The blood on the surface of the organs was blotted with filter paper and weighed separately to calculate the organ coefficients.

[0057] Blood index detection: 24 hours after the last administration, blood was collected from the mouse orbital area, EDTA-2K anticoagulated blood was collected, and blood cell count was performed using a blood analyzer to detect white blood cells (WBC) and red blood cells (RBC), platelets (PLT), lymphocyte percentage (LY%) and neutrophil ratio (NE%), etc.

[0058] Liver histopathological examination: Histopathological observation was performed. The weighed liver was fixed in 4% formaldehyde solution, embedded in paraffin, sliced ​​and stained with hematoxylin-eosin (HE). The histomorphological changes of spleen and thymus were observed under a microscope.

[0059] Mouse blood biochemical detection: EDTA-2K anticoagulated blood was collected and blood biochemical (IFN-γ, IL-6, TNF-α) detection was performed using a blood biochemical detector.

[0060] Experimental results:

[0061] Depend on Figure 1 It can be seen that the body weight of the blank group was stable after modeling, and the body weight of the model group and each group treated with the Chinese medicine composition decreased over time, with the model group and the low-dose group decreasing the most, while the high-dose group of the Chinese medicine composition decreased the least.

[0062] Depend on Figure 2 It can be seen that compared with the sham operation group, the liver organ coefficient of the model group increased significantly (##p<0.01), and the model was successfully prepared; compared with the model group, the high dose of the Chinese medicine composition can significantly reduce the liver organ coefficient (*p<0.05).

[0063] Depend on Figure 3It can be seen that compared with the sham operation group, RET-He, WBC, NEUT#, and MONO% in the model group increased significantly (##p<0.01), and MFR in the model group decreased significantly (##p<0.01), and the model was successfully prepared; compared with the model group, the high dose of the Chinese medicine composition can significantly reduce RET-He, WBC, NEUT#, and MONO%, and increase MFR (*p<0.05).

[0064] Figure 4 The liver tissue damage of cyclophosphamide model mice was observed by HE staining. The results showed that the liver tissue sections of the control group mice showed normal liver morphology, hepatocytes were arranged in an orderly manner, the central vein was clearly visible, and no signs of necrosis or inflammation were observed. The cyclophosphamide-treated group showed obvious liver damage morphology, hepatocyte necrosis, inflammatory cell infiltration and liver structural destruction. The high-dose group of the Chinese medicine composition can significantly reduce the number of vacuolated hepatocytes. Compared with the model group, the integrity of the liver lobule structure in the high-dose group of the composition was improved to a certain extent. At the same time, hepatocyte necrosis was reduced, inflammatory infiltration was reduced, and the hepatocyte structure was significantly improved.

[0065] Depend on Figure 5 It can be seen that compared with the sham operation group, the ALT, AST, IFN-γ, TNF-α, and IL-6 levels in the model group increased significantly (##p<0.01), and the model was successfully prepared; compared with the model group, each dose of the composition can significantly reduce the serum ALT, AST, IFN-γ, TNF-α, and IL-6 levels (**p<0.01).

[0066] By comparing the data of the treatment group, the model group and the control group, the therapeutic effect of the Chinese medicine composition on liver injury was analyzed. The results showed that the weight loss of the animals in the high-dose group was better than that in the model group, the organ coefficients in the high-dose group were significantly lower than those in the model group, the liver function indexes were significantly improved, the liver tissue pathological damage was alleviated, and the hematological indexes tended to be normal, indicating that the Chinese medicine composition of the present invention has a good therapeutic effect on liver injury caused by cyclophosphamide.

Claims

1. A Chinese medicine composition for treating liver damage, characterized in that: It includes astragalus, angelica, ophiopogon, schisandra chinensis, wolfberry, chrysanthemum and codonopsis.

2. A Chinese medicine composition for treating liver damage according to claim 1, characterized in that: The invention comprises the following Chinese medicinal raw materials in parts by weight: 10-30 parts of astragalus, 3-6 parts of angelica, 5-15 parts of ophiopogon, 2-8 parts of schisandra chinensis, 5-15 parts of wolfberry, 5-15 parts of chrysanthemum and 5-15 parts of codonopsis pilosula.

3. A Chinese medicine composition for treating liver damage according to claim 2, characterized in that: The invention comprises the following Chinese medicinal raw materials in parts by weight: 15-25 parts of astragalus, 4-6 parts of angelica, 8-12 parts of ophiopogon, 4-7 parts of schisandra chinensis, 8-12 parts of wolfberry, 8-12 parts of chrysanthemum and 8-12 parts of codonopsis pilosula.

4. A Chinese medicine composition for treating liver damage, characterized in that: The invention is prepared from the following Chinese medicinal raw materials in parts by weight: 20 parts of astragalus, 4 parts of angelica, 10 parts of ophiopogon, 5 parts of vinegar schisandra, 10 parts of wolfberry, 10 parts of chrysanthemum and 10 parts of codonopsis.

5. The method for preparing the Chinese medicine composition for treating liver damage according to any one of claims 1 to 4, characterized in that: The following steps are involved: Step 1: Soak astragalus, angelica, ophiopogon, vinegar schisandra, wolfberry, chrysanthemum and codonopsis in water, reflux extraction, filter, combine the extracts, and obtain liquid A; Step 2: Add water to the residue after filtering the above medicinal liquid, decoct and extract, filter, and combine the filtrate to obtain medicinal liquid B; Step 3: Mix the medicinal solution A and the medicinal solution B, and concentrate them to obtain a decoction.

6. The method for preparing the Chinese medicine composition for treating liver injury according to claim 5, characterized in that: The following steps are involved: Step 1: add 6 to 12 times the amount of water according to the mass volume ratio to Astragalus, Angelica, Ophiopogon, Schisandrae Chrysanthemum, Lycium barbarum, Chrysanthemum and Codonopsis, soak for 0.5 to 3 hours, reflux extraction for 1 to 2 hours, extract continuously for 2 to 3 times, filter the extract, separate the supernatant from the residual drug residue, and obtain drug solution A; Step 2: Add 8 to 10 times the amount of water to the residue after filtering the medicinal liquid according to the mass volume ratio, and boil and extract for 1 to 2 hours to obtain medicinal liquid B; Step 3: Mix the drug solution A and the drug solution B, and concentrate them to obtain an extract.

7. A Chinese medicine preparation of a Chinese medicine composition for treating liver damage, characterized in that: The Chinese medicine extract and a pharmaceutically acceptable carrier are prepared into tablets, granules, capsules, pills, powders, pastes or oral liquids.

8. The Chinese medicine preparation of the Chinese medicine composition for treating liver damage according to claim 7, characterized in that: The preparation method of the granules is: Step 1: Take the Chinese medicine extract obtained in claim 5 or 6, dry it under reduced pressure at 80°C to obtain a dry powder of the drug extract, then add appropriate amounts of microcrystalline cellulose, dextrin and other auxiliary materials according to the ratio of the composition of dry powder of extract: dextrin: microcrystalline cellulose = 10:7:3, mix evenly, add a certain amount of wetting agent to make a soft material, sieve through No. 2 to granulate, and dry at 60°C to constant weight; Step 2: Use a suitable granule filling machine to fill the dried granules into packaging bags.

9. The Chinese medicine preparation of the Chinese medicine composition for treating liver damage according to claim 7, characterized in that: The preparation method of the capsule is as follows: Step 1: Take the Chinese medicine extract obtained in claim 5 or 6, dry it under reduced pressure at 80°C to obtain a dry powder of the drug extract, then add appropriate amounts of microcrystalline cellulose, dextrin and other auxiliary materials according to the ratio of the composition of dry powder of extract: dextrin: microcrystalline cellulose = 10:7:3, mix evenly, add a certain amount of wetting agent to make a soft material, pass through a No. 2 sieve, granulate, and dry at 60°C to constant weight; Step 2: Use a suitable capsule filling machine to fill the dried granules into capsules.

10. Use of the Chinese medicine composition according to any one of claims 1 to 4 in the preparation of a product for treating liver injury, wherein the liver injury includes drug-induced liver injury, chemical liver injury, alcoholic liver injury, and viral hepatitis.

11. The use according to claim 10, characterized in that: The drug-induced liver injury includes drug-induced liver injury caused by cyclophosphamide.

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