Pharmaceutical composition containing dendrobium nobile stem, caulis spatholobi, saffron crocus and anoectochilus roxburghii medicinal materials

Abstract

The invention discloses a traditional Chinese medicine composition with an anti-gastritis effect. The traditional Chinese medicine composition is prepared from four traditional Chinese medicinal materials including dendrobium nobile, caulis sinomenii, saffron crocus and anoectochilus formosanus. The traditional Chinese medicine composition has good effects of resisting inflammation and improving the gastric emptying rate.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition with anti-gastritis effect. The composition is composed of four traditional Chinese medicinal materials: dendrobium nobile, clematis sylvestris, saffron and roxburghii, and has good anti-inflammatory and gastric emptying rate improving effects. Background Art

[0002] Gastritis, which is inflammation of the gastric mucosa, is divided into two categories: acute gastritis and chronic gastritis. Most gastritis is not contagious, and its clinical symptoms mainly include upper abdominal discomfort, such as bloating and burning sensation, accompanied by nausea, vomiting, loss of appetite and other symptoms. Some patients do not have obvious discomfort. For gastritis caused by Helicobacter pylori infection, a combination of antibiotics is usually used to eradicate the bacteria; non-infectious gastritis focuses on protecting the gastric mucosa and improving eating habits and lifestyle. Most gastritis has a good prognosis, but if chronic gastritis is not properly managed for a long time, it may progress to gastric ulcers or even gastric cancer.

[0003] Gastritis can generally be treated by taking gastric mucosal protectants, drugs that promote gastric motility, and antibiotics. Common gastric mucosal protectants include potassium citrate bismuth tablets, aluminum carbonate magnesium chewable tablets, colloidal bismuth tartrate capsules, etc. These drugs can cover the surface of the gastric mucosa to prevent irritants from irritating the gastric mucosa; drugs that promote gastric motility include domperidone tablets, mosapride citrate tablets, itopride hydrochloride tablets, etc., which help empty the stomach and are mainly used to treat symptoms such as nausea, vomiting, bloating, and hiccups caused by gastritis; some patients may experience gastritis due to Helicobacter pylori infection, and need to take clarithromycin tablets, erythromycin tablets, and cephalexin capsules as prescribed by the doctor, and cooperate with proton pump inhibitors, such as omeprazole enteric-coated capsules and lansoprazole enteric-coated tablets. Long-term use of these drugs may have side effects such as decreased appetite, abdominal pain, diarrhea, allergic reactions, and impaired liver function. Traditional Chinese medicine has a good therapeutic effect in improving spleen and stomach function, so the development of a Chinese medicine composition with anti-gastritis effect has very important clinical value.

[0004] Dendrobium nobile Lindl is the stem of Dendrobium nobile Lindl, a plant of the genus Dendrobium in the Orchidaceae family. It has the effects of clearing away heat and nourishing yin, moistening the lungs and producing body fluid, benefiting the kidneys and nourishing the stomach, improving eyesight and strengthening the waist. It is mainly used to treat heat diseases that damage body fluids, dry mouth and thirst, dry coughs due to lung heat, insufficient stomach yin, calming the nerves and relieving convulsions, stomach pain and dry retching, etc.

[0005] Cocculus orbiculatus is the dried stem or root of Cocculus orbiculatus (L.) DC. of the Menispermaceae family. It tastes bitter, pungent and cold. It enters the lung, spleen, kidney and bladder meridians. It is used to treat rheumatic joint pain, intercostal neuralgia, acute nephritis, urinary tract infection, hypertension, rheumatic heart disease, edema, and can also treat snake bites.

[0006] Saffron (Crocus sativus L.), also known as saffron and saffron, is a perennial herb of the genus Crocus in the family Iridaceae. According to the Pharmacopoeia of the People's Republic of China, its medicinal part is the dried style. As a traditional Chinese medicine, saffron has the following effects: promoting blood circulation and removing blood stasis, cooling blood and detoxifying, relieving depression and calming the mind. It is used for amenorrhea, postpartum blood stasis, fever and rashes, depression, palpitations and madness, etc.

[0007] Anoectochilus roxburghii (Wall.) Lindl. and Anoectochilus formosanus Hayata. It has the effects of clearing heat, cooling blood, removing dampness and detoxifying. It is mainly used to treat lung heat cough, hemoptysis due to pulmonary tuberculosis, hematuria, infantile convulsions, tetanus, nephritic edema, rheumatic pain, traumatic injury, and snake bites.

[0008] Based on the above reasons, the present invention provides a traditional Chinese medicine composition with anti-gastritis effect. The traditional Chinese medicine composition is composed of Dendrobium nobile, Caulis dahuricae, Saffron and Anoectochilus roxburghii. The traditional Chinese medicine composition has good anti-inflammatory and gastric emptying rate improvement effects, and can be used in health products for improving spleen and stomach function. Summary of the invention

[0009] The main purpose of the present invention is to provide a traditional Chinese medicine composition with definite efficacy, few side effects and natural safety for anti-gastritis, so as to solve the problems of poor effect or easy side effects of common gastritis treatment products in the prior art.

[0010] In order to achieve the above object, according to one aspect of the present invention, a traditional Chinese medicine composition with anti-gastritis effect is provided, characterized in that the traditional Chinese medicine composition is 20 parts of Dendrobium nobile, 10 parts of Caulis dahuricae, 30 parts of Saffron and 10 parts of Anoectochilus roxburghii.

[0011] Furthermore, the Chinese medicine composition can be prepared by the following method: weigh each medicinal material according to the prescription, crush it into coarse powder, and mix it evenly. According to the total amount of medicinal materials in each group, add 16 times the amount of water, decoct for 1 hour, filter, and collect the filtrate. Add 12 times the amount of water of the original medicinal material to the residue, continue to decoct for 1 hour, filter, combine the two filtrates, and concentrate to 1.2 times the volume of the original medicinal material (volume / mass ratio), place it to about 50°C, add 95% ethanol while hot to an alcohol concentration of about 80%, let it stand overnight, take the supernatant, reduce pressure to recover ethanol and concentrate, and dry the concentrate at 60°C under reduced pressure.

[0012] According to another aspect of the present invention, there is provided a use of the above-mentioned Chinese medicine composition in a drug or health product for preventing or treating gastritis.

[0013] Beneficial effects of the present invention:

[0014] The technical scheme of the present invention is applied, and the Chinese medicine composition of the present invention is composed of 20 parts of Dendrobium nobile, 10 parts of Caulis dahuricae, 30 parts of Crocus saffron and 10 parts of Anoectochilus roxburghii. The Chinese medicine composition has good anti-inflammatory and gastric emptying rate improving effects, and well solves the problems of poor effects and easy side effects of common anti-gastritis products. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solution in this embodiment, the drawings required for use in the description of the embodiment will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 It is a schematic diagram showing the effect of a composition of this example on the gastric emptying rate of rats. DETAILED DESCRIPTION

[0017] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0018] Example 1 Preparation of Chinese medicine composition 1-7

[0019] The Chinese medicine compositions 1-7 of the present invention adopt a uniform design experimental scheme: referring to the literature (Liang Maoxin, Li Dongan. Improved application of uniform design in the selection of small Chinese medicine compound prescriptions [J]. World Science and Technology - Modernization of Traditional Chinese Medicine, 2006, 8 (02): 53-56.), the uniform design scheme U7 (7 4 )(As shown in Table 1), four Chinese medicinal materials, namely, Dendrobium nobile, Thunbergia pyrifera, Saffron and Anoectochilus roxburghii, were used as investigation factors in the prescription. Each factor had 7 levels (i.e., 7 different doses with increasing gradient). The investigation dose was between 0 and 30 g. Factors and levels were arranged according to the uniform design table, and prescription design was carried out to construct a total of 7 groups of Chinese medicine compositions.

[0020] Table 1 Chinese medicine composition U7(7 4 )Uniform Design Table

[0021]

[0022] Preparation of Chinese medicine composition 1-7 According to the amount of medicinal materials of composition 1-7 in Table 1, each medicinal material is weighed according to the prescription of each group, crushed into coarse powder, and mixed evenly. According to the total amount of medicinal materials in each group, 16 times the amount of water is added, decocted for 1 hour, filtered, and the filtrate is collected. Add 12 times the amount of water of the original medicinal material to the dregs, continue to decoct for 1 hour, filter, combine the filtrates twice, and concentrate to 1.2 times the volume of the original medicinal material (volume / mass ratio), place to about 50°C, add 95% ethanol while hot to an alcohol concentration of about 80%, stand overnight, take the supernatant, reduce pressure to recover ethanol and concentrate, and reduce pressure and dry the concentrate at 60°C to obtain the extract of Chinese medicine composition 1-7.

[0023] Example 2 Evaluation of anti-inflammatory activity of Chinese medicine compositions 1-7

[0024] Experimental Drugs Test drug compositions 1 to 7 were prepared according to the method of Example 1.

[0025] Experimental method: Raw264.7 cells in the logarithmic growth phase were taken and prepared into a single cell suspension using complete medium containing 10% FBS and 1% streptomycin. The concentration was adjusted to 10 5 / mL, inoculated in a 96-well plate, cultured for 18 hours, and after the cells adhered to the wall, 50 mg / mL of the extracts of the Chinese medicine composition 1-7 were added, and after pretreatment for 5 hours, 1.0 μg / mL of lipopolysaccharide (LPS) was added. After incubation for 24 hours, the 96-well plate was placed on a centrifuge and centrifuged at 1000×g for 10 minutes. The supernatant was taken and the NO content was determined according to the instructions of the nitric oxide kit (Shanghai ELISA Biotechnology Co., Ltd.). The supernatant was taken separately and the contents of inflammatory factors IL-6, IL-1β, and TNF-α were determined according to the instructions of the enzyme-linked immunosorbent assay kit (Shanghai ELISA Biotechnology Co., Ltd.). The results are shown in Table 2.

[0026] Table 2 Effects of different compositions on NO and inflammatory factors in RAW264.7 cells induced by lipopolysaccharide (n=5)

[0027]

[0028]

[0029] Note: Compared with the model group, *p<0.05;

[0030] Experimental results From the data in Table 2, it can be seen that Chinese medicine composition 5 and Chinese medicine composition 7 can effectively inhibit the release of cellular NO and inflammatory factors, and show good anti-inflammatory activity. In particular, Chinese medicine composition 5 (composed of 20 parts of Dendrobium nobile, 10 parts of Rhizoma Cyperi, 30 parts of Saffron and 10 parts of Anoectochilus roxburghii) has the strongest anti-inflammatory activity.

[0031] Example 2 Effect of Chinese medicine composition 5 on gastrointestinal function

[0032] Thirty SD rats were randomly divided into 6 groups, 5 rats in each group, including: normal group, model group, positive control group, high, medium and low dose groups of composition 5. The normal group was given 1 mL / 100 g of normal saline by gavage for 2 consecutive weeks, and the other groups were given equal volume of lard by gavage for 2 consecutive weeks to create an animal model of food accumulation. After 2 weeks, the positive control group was given 3.125 mg / kg of domperidone solution by gavage, and the high, medium and low dose groups of composition 5 were given 0.2 g / kg, 1 g / kg and 5 g / kg by gavage, respectively. The normal group and the model group continued to be given equal volume of normal saline by gavage, once a day. After 2 consecutive weeks of administration, the rats were fasted for 12 hours and then given 1 mL / 100 g of carbon ink by gavage. After 30 minutes, the rats were killed, dissected and the stomachs were taken. After weighing the total weight of the stomach, cut the stomach open, wash out the stomach contents, wipe dry and weigh the net weight of the stomach. Calculate the gastric emptying rate according to the formula: gastric emptying rate (%) = [1-(total weight of stomach-net weight of stomach) / gastric weight] × 100%. Figure 1 shown. Figure 1 The effect of the composition on gastric emptying rate of rats, compared with the normal group, *p<0.05; compared with the model group, #p<0.05;

[0033] Depend on Figure 1 The data show that Chinese medicine composition 5 (composed of 20 parts of Dendrobium nobile, 10 parts of Caulis dahurica, 30 parts of Saffron and 10 parts of Anoectochilus roxburghii) has a good effect in improving the gastric emptying rate of rats.

[0034] Example 3 Preparation of granules

[0035] 20 parts of Dendrobium nobile, 10 parts of Caulis dahuricae, 30 parts of Saffron and 10 parts of Anoectochilus roxburghii in weight ratio were crushed into coarse powder and mixed evenly. According to the total amount of medicinal materials in each group, 16 times of water were added respectively, decocted for 1 hour, filtered, and the filtrate was collected. 12 times of water were added to the medicinal residues, and the decocted for 1 hour, filtered, and the filtrate was combined twice, and concentrated to 1.2 times the volume (volume / mass ratio) of the amount of the original medicinal materials, placed to about 50°C, 95% ethanol was added while hot to an alcohol concentration of about 80%, and stood overnight, the supernatant was taken to recover ethanol under reduced pressure and concentrated, and after the concentrated solution was dried under reduced pressure at 60°C, one or more of the auxiliary materials such as starch, microcrystalline cellulose, lactose, etc. were added, mixed, granulated, dried, and granulated to form the granules of the present invention.

[0036] The above is only an embodiment of the present invention, and the common knowledge such as the known specific structure and characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several modifications and improvements can be made without departing from the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

[0037] The embodiments of the present invention are described in detail above. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. At the same time, changes or deformations made by those skilled in the art based on the ideas of the present invention, the specific implementation methods and application scope of the present invention, all belong to the scope of protection of the present invention. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A Chinese medicine composition having an anti-gastritis effect, characterized in that: The traditional Chinese medicine composition consists of 20 parts of dendrobium nobile, 10 parts of clematis dactylis, 30 parts of saffron and 10 parts of roxburghii.

2. The Chinese medicine composition according to claim 1, characterized in that: The preparation method of the Chinese medicine composition comprises: weighing each medicinal material according to the prescription, crushing it into coarse powder, and mixing it evenly. According to the total amount of medicinal materials in each group, add 16 times the amount of water, decoct for 1 hour, filter, and collect the filtrate. Add 12 times the amount of water of the original medicinal material to the medicinal residue, continue to decoct for 1 hour, filter, combine the two filtrates, and concentrate to 1.2 times the volume of the original medicinal material (volume / mass ratio), place it to about 50°C, add 95% ethanol while hot to an alcohol concentration of about 80%, stand it overnight, take the supernatant, reduce pressure to recover ethanol and concentrate it, and reduce pressure and dry the concentrated solution at 60°C to obtain it.

3. Use of the Chinese medicine composition according to claim 1 or 2 in drugs or health products for preventing or treating gastritis.

4. An anti-gastritis pharmaceutical composition comprising the pharmaceutical composition according to claims 1 to 3 and pharmaceutically acceptable excipients.

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