Traditional Chinese medicine preparation for nourishing hair and preparation method thereof

By preparing a traditional Chinese medicine preparation containing a variety of traditional Chinese herbal medicines, the problems of harmful ingredients and temporary effects of existing hair dyes have been solved, and the effect of fundamentally promoting hair care and continuous black hair care has been achieved.

CN119818635APending Publication Date: 2025-04-15BEIJING XIANGYUN INTERNATIONAL HEALTH TECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411724225.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-28
Publication Date
2025-04-15

AI Technical Summary

Technical Problem

Existing hair dyes contain a large number of harmful ingredients. Long-term use is not good for human health, and can only achieve temporary and superficial improvements, and cannot fundamentally solve the problem of gray hair.

Method used

By preparing a traditional Chinese medicine preparation containing ginger extract, wolfberry, astragalus, Codonopsis pilosula, hair follicle activator, Ligustrum, Rehmannia ulmoide, Eucommia ulmoide, red dates, Polygonatum, Polygonatum, Polygonatum, donkey-hide gelatin, carrageenan and black fungus, these traditional Chinese herbal medicines have the effects of replenishing blood and nourishing kidney yang, and promote hair follicles and achieve hair nourishment.

Benefits of technology

This traditional Chinese medicine preparation promotes hair care, uses pure Chinese herbal medicines to regulate kidney function, realizes continuous maintenance of black hair, significantly improves hair care efficiency, and is harmless and safe and reliable.

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Abstract

The invention discloses a traditional Chinese medicine preparation for nourishing hair and a preparation method of the traditional Chinese medicine preparation. The traditional Chinese medicine preparation is prepared from a ginger extracting solution, fructus lycii, radix astragali, radix codonopsis, a hair follicle activating agent, fructus ligustri lucidi, radix rehmanniae preparata, cortex eucommiae, red dates, rhizoma polygonati, radix polygoni multiflori, colla corii asini, carrageenan and black The invention relates to the technical field of traditional Chinese medicine preparations. According to the traditional Chinese medicine preparation for nourishing hair and the preparation method thereof, the fresh ginger extracting solution and the hair follicle activating agent are used for promoting hair nourishing, the fructus lycii, the prepared rehmannia root and the cortex eucommiae are used for nourishing the kidney and reinforcing essence and blood output, the red dates, the radix polygoni multiflori, the colla corii asini and the black sesame powder are used for replenishing blood, and the hair nourishing efficiency can be fundamentally accelerated; the pure traditional Chinese medicine preparation is adopted, the kidney function is effectively conditioned, stable guarantee is continuously provided for black hair after hair nourishing, and the hair nourishing efficiency is more remarkable through internal and external matched use of the soaking preparation and the granules.
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Description

Technical Field

[0001] The invention relates to the technical field of Chinese medicine preparations, in particular to a Chinese medicine preparation for hair care and a preparation method thereof. Background Art

[0002] Traditional Chinese medicine believes that the reason for black hair to turn white is kidney deficiency, blood deficiency, spleen deficiency, lung deficiency, heart deficiency, blood stasis, blood heat, wind evil, liver depression, emotional disorder, etc.; Western medicine believes that the formation of white hair is caused by heredity, or due to lack of tyrosinase, which reduces the synthesis of melanin, or due to skin and hair follicle diseases, or due to lack of protein, trace elements and amino acids. Obviously, white hair reflects a person's health status, aging degree and aesthetic deficiencies to a certain extent.

[0003] The appearance of gray hair seriously affects people's appearance. The existing methods for turning gray hair into black hair are mostly to achieve the effect of hair care by directly dyeing the hair. However, many hair dyes contain a large amount of harmful ingredients, and long-term use is not good for human health. Some even cause cancer. In addition, hair care by dyeing can only achieve temporary and superficial changes. Therefore, a Chinese medicine preparation for hair care and a preparation method thereof are proposed, which can achieve hair care by promoting hair follicle activity and replenishing blood and qi. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a hair-nourishing Chinese medicinal preparation and a preparation method thereof.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0006] On the one hand, a traditional Chinese medicine preparation for hair care comprises the following raw materials by weight: 10 parts of ginger extract, 20 parts of wolfberry, 40 parts of astragalus, 10 parts of codonopsis, 100 parts of hair follicle activator, 12 parts of Ligustrum lucidum, 18 parts of rehmannia root, 10 parts of eucommia bark, 20 parts of red dates, 20 parts of polygonatum, 15 parts of polygonum multiflorum, 30 parts of donkey-hide gelatin, 30 parts of carrageenan, and 10 parts of black fungus.

[0007] The present invention is further configured as follows: the method for obtaining the ginger extract comprises:

[0008] Take young ginger planted for four months, peel and crush it, add twice the amount of deionized water, add three times the amount of ethanol, stir well and let it stand for 24-48 hours, and obtain the extract and solid raw ginger after centrifugation;

[0009] Drying the solid raw material ginger and grinding it into powder to obtain ginger powder;

[0010] The extract is placed in an ethanol recovery device for heating to recover ethanol, and then the remaining ethanol in the extract is heated to volatilize, ginger powder is added, and the mixture is stirred evenly to obtain ginger extract.

[0011] The present invention is further configured as follows: the hair follicle activator comprises a cleaning solution and a semi-solid activator, and the preparation method of the cleaning solution and the semi-solid activator comprises:

[0012] Weigh equal weights of black rice flour, black bean flour, mulberry, black sesame flour, walnut flour and olive oil, place in a heating kettle with a stirring mechanism, add five times the total weight of water and boil over high heat for 1-2 hours, then simmer over low heat until the total volume is reduced to half of the initial volume, let stand for 3-5 hours, vacuum the upper clear liquid to obtain the cleaning liquid, vacuum the remaining liquid to obtain the semi-solid activator.

[0013] The present invention is further configured as follows: the Chinese medicinal preparation for hair care is a granule preparation and an immersion preparation.

[0014] On the other hand, a method for preparing a hair-nourishing Chinese medicine preparation specifically comprises the following steps:

[0015] Step 1, according to the weight ratio of 10:20:40:10:100:12:18:10:20:20:15:30:30:10, ginger extract, wolfberry, astragalus, codonopsis, hair follicle activator, Ligustrum lucidum, Rehmannia root, Eucommia bark, red dates, Polygonatum sibiricum, Polygonum multiflorum, donkey-hide gelatin, carrageenan and black fungus are weighed in sequence, and the hair follicle activator is removed to obtain the boiling raw materials;

[0016] Step 2: Add three times the amount of deionized water to the boiled raw materials and boil them over high heat for 2-3 hours, filter out the boiled raw materials to obtain liquid A, add two times the amount of deionized water to the filtered boiled raw materials, boil them over low heat for 1-2 hours, filter out impurities, and obtain liquid B;

[0017] Step 3: Mix half of liquid B with liquid A, record the volume as C, add semi-solid activator, boil over high heat for 30-45 minutes, simmer the liquid to a volume of D, where the volume D is one third of the volume C, and spray dry to obtain granules;

[0018] Step 4: Mix the remaining half of liquid B with the cleaning solution, boil over high heat, and then simmer over low heat for 0.5 h to obtain the soaking preparation.

[0019] Among them, red dates, Polygonum multiflorum, donkey-hide gelatin and black sesame powder all have the characteristics of nourishing blood. Under the theory that hair is the rest of blood, they nourish the hair and effectively promote hair growth.

[0020] Among them, wolfberry, rehmannia root and eucommia all have nourishing properties for the kidneys. According to the theory that the kidneys store essence and essence produces blood, they have the advantages of strengthening the foundation and replenishing the vital energy.

[0021] Fructus Ligustri Lucidi can not only make hair black, but also has the advantage of nourishing essence and yin.

[0022] The present invention provides a hair-nourishing Chinese medicine preparation and a preparation method thereof. It has the following beneficial effects:

[0023] The present invention promotes hair care through ginger extract and hair follicle activator, and uses wolfberry, rehmannia root and eucommia bark to nourish the kidneys, while reinforcing the output of essence and blood, and uses red dates, polygonum multiflorum, donkey-hide gelatin and black sesame powder to replenish blood, which can fundamentally accelerate the efficiency of hair care, and uses pure Chinese medicine preparations to effectively regulate kidney function, and continuously provide stable guarantee for black hair after hair care, and through the internal and external coordinated use of soaking preparations and granules, the hair care efficiency is more significant. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention will be described clearly and completely below.

[0025] The embodiment of the present invention provides the following technical solutions:

[0026] Embodiment 1: A Chinese medicine preparation for hair care, comprising: ginger extract, wolfberry, astragalus, codonopsis, hair follicle activator, Ligustrum lucidum, rehmannia root, eucommia bark, red dates, polygonatum, polygonum multiflorum, donkey-hide gelatin, carrageenan and black fungus, and the ginger extract, wolfberry, astragalus, codonopsis, hair follicle activator, Ligustrum lucidum, rehmannia root, eucommia bark, red dates, polygonatum, polygonum multiflorum, donkey-hide gelatin, carrageenan and black fungus are in the following weight ratios: 5:10:50:15:50:7:26:15:10:30:10:20:40:5.

[0027] Among them, the methods of obtaining ginger extract include:

[0028] Take young ginger planted for four months, peel and crush it, add twice the amount of deionized water, add three times the amount of ethanol, stir well and let it stand for 24-48 hours, and obtain the extract and solid raw ginger after centrifugation;

[0029] Drying the solid raw material ginger and grinding it into powder to obtain ginger powder;

[0030] The extract is placed in an ethanol recovery device for heating to recover ethanol, and then the remaining ethanol in the extract is heated to volatilize, ginger powder is added, and the mixture is stirred evenly to obtain ginger extract.

[0031] The hair follicle activator includes a cleaning solution and a semi-solid activator, and the preparation methods of the cleaning solution and the semi-solid activator include:

[0032] Weigh equal weights of black rice flour, black bean flour, mulberry, black sesame flour, walnut flour and olive oil, place in a heating kettle with a stirring mechanism, add five times the total weight of water and boil over high heat for 1-2 hours, then simmer over low heat until the total volume is reduced to half of the initial volume, let stand for 3-5 hours, vacuum the upper clear liquid to obtain the cleaning liquid, vacuum the remaining liquid to obtain the semi-solid activator.

[0033] The preparation method of the above-mentioned Chinese medicine preparation is characterized in that it specifically comprises the following steps:

[0034] Step 1, according to the weight ratio of 5:10:50:15:50:7:26:15:10:30:10:20:40:5, ginger extract, wolfberry, astragalus, codonopsis, hair follicle activator, Ligustrum lucidum, Rehmannia root, Eucommia bark, red dates, Polygonatum sibiricum, Polygonum multiflorum, donkey-hide gelatin, carrageenan and black fungus, after screening out the hair follicle activator, obtain the boiling raw materials;

[0035] Step 2: Add three times the amount of deionized water to the boiled raw materials and boil them over high heat for 2-3 hours, filter out the boiled raw materials to obtain liquid A, add two times the amount of deionized water to the filtered boiled raw materials, boil them over low heat for 1-2 hours, filter out impurities, and obtain liquid B;

[0036] Step 3: Mix half of liquid B with liquid A, record the volume as C, add semi-solid activator, boil over high heat for 30-45 minutes, simmer the liquid to a volume of D, where the volume D is one third of the volume C, and spray dry to obtain granules;

[0037] Step 4: Mix the remaining half of liquid B with the cleaning solution, boil over high heat, and then simmer over low heat for 0.5 h to obtain the soaking preparation.

[0038] Embodiment 2: A Chinese medicine preparation for hair care, comprising: ginger extract, wolfberry, astragalus, codonopsis, hair follicle activator, Ligustrum lucidum, rehmannia root, eucommia bark, red dates, polygonatum, polygonum multiflorum, donkey-hide gelatin, carrageenan and black fungus, and the ginger extract, wolfberry, astragalus, codonopsis, hair follicle activator, Ligustrum lucidum, rehmannia root, eucommia bark, red dates, polygonatum, polygonum multiflorum, donkey-hide gelatin, carrageenan and black fungus are in the following weight ratios: 10:20:40:10:100:12:18:10:20:20:15:30:30:10.

[0039] Among them, the methods of obtaining ginger extract include:

[0040] Take young ginger planted for four months, peel and crush it, add twice the amount of deionized water, add three times the amount of ethanol, stir well and let it stand for 24-48 hours, and obtain the extract and solid raw ginger after centrifugation;

[0041] Drying the solid raw material ginger and grinding it into powder to obtain ginger powder;

[0042] The extract is placed in an ethanol recovery device for heating to recover ethanol, and then the remaining ethanol in the extract is heated to volatilize, ginger powder is added, and the mixture is stirred evenly to obtain ginger extract.

[0043] The hair follicle activator includes a cleaning solution and a semi-solid activator, and the preparation methods of the cleaning solution and the semi-solid activator include:

[0044] Weigh equal weights of black rice flour, black bean flour, mulberry, black sesame flour, walnut flour and olive oil, place in a heating kettle with a stirring mechanism, add five times the total weight of water and boil over high heat for 1-2 hours, then simmer over low heat until the total volume is reduced to half of the initial volume, let stand for 3-5 hours, vacuum the upper clear liquid to obtain the cleaning liquid, vacuum the remaining liquid to obtain the semi-solid activator.

[0045] The preparation method of the above-mentioned Chinese medicine preparation is characterized in that it specifically comprises the following steps:

[0046] Step 1, according to the weight ratio of 10:20:40:10:100:12:18:10:20:20:15:30:30:10, ginger extract, wolfberry, astragalus, codonopsis, hair follicle activator, Ligustrum lucidum, Rehmannia root, Eucommia bark, red dates, Polygonatum sibiricum, Polygonum multiflorum, donkey-hide gelatin, carrageenan and black fungus are weighed in sequence, and the hair follicle activator is removed to obtain the boiling raw materials;

[0047] Step 2: Add three times the amount of deionized water to the boiled raw materials and boil them over high heat for 2-3 hours, filter out the boiled raw materials to obtain liquid A, add two times the amount of deionized water to the filtered boiled raw materials, boil them over low heat for 1-2 hours, filter out impurities, and obtain liquid B;

[0048] Step 3: Mix half of liquid B with liquid A, record the volume as C, add semi-solid activator, boil over high heat for 30-45 minutes, simmer the liquid to a volume of D, where the volume D is one third of the volume C, and spray dry to obtain granules;

[0049] Step 4: Mix the remaining half of liquid B with the cleaning solution, boil over high heat, and then simmer over low heat for 0.5 h to obtain the soaking preparation.

[0050] Embodiment 3, a Chinese medicine preparation for hair care, comprising: ginger extract, wolfberry, astragalus, codonopsis, hair follicle activator, Ligustrum lucidum, rehmannia root, eucommia bark, red dates, polygonatum, polygonum multiflorum, donkey-hide gelatin, carrageenan and black fungus, and the ginger extract, wolfberry, astragalus, codonopsis, hair follicle activator, Ligustrum lucidum, rehmannia root, eucommia bark, red dates, polygonatum, polygonum multiflorum, donkey-hide gelatin, carrageenan and black fungus include the following weight ratios: 15:30:20:5:150:18:12:5:30:10:20:40:15:15.

[0051] Among them, the methods of obtaining ginger extract include:

[0052] Take young ginger planted for four months, peel and crush it, add twice the amount of deionized water, add three times the amount of ethanol, stir well and let it stand for 24-48 hours, and obtain the extract and solid raw ginger after centrifugation;

[0053] Drying the solid raw material ginger and grinding it into powder to obtain ginger powder;

[0054] The extract is placed in an ethanol recovery device for heating to recover ethanol, and then the remaining ethanol in the extract is heated to volatilize, ginger powder is added, and the mixture is stirred evenly to obtain ginger extract.

[0055] The hair follicle activator includes a cleaning solution and a semi-solid activator, and the preparation methods of the cleaning solution and the semi-solid activator include:

[0056] Weigh equal weights of black rice flour, black bean flour, mulberry, black sesame flour, walnut flour and olive oil, place in a heating kettle with a stirring mechanism, add five times the total weight of water and boil over high heat for 1-2 hours, then simmer over low heat until the total volume is reduced to half of the initial volume, let stand for 3-5 hours, vacuum the upper clear liquid to obtain the cleaning liquid, vacuum the remaining liquid to obtain the semi-solid activator.

[0057] The preparation method of the above-mentioned Chinese medicine preparation is characterized in that it specifically comprises the following steps:

[0058] Step 1, according to the weight ratio of 15:30:20:5:150:18:12:5:30:10:20:40:15:15, ginger extract, wolfberry, astragalus, codonopsis, hair follicle activator, Ligustrum lucidum, Rehmannia root, Eucommia bark, red dates, Polygonatum sibiricum, Polygonum multiflorum, donkey-hide gelatin, carrageenan and black fungus are weighed in sequence, and the hair follicle activator is removed to obtain the boiling raw materials;

[0059] Step 2: Add three times the amount of deionized water to the boiled raw materials and boil them over high heat for 2-3 hours, filter out the boiled raw materials to obtain liquid A, add two times the amount of deionized water to the filtered boiled raw materials, boil them over low heat for 1-2 hours, filter out impurities, and obtain liquid B;

[0060] Step 3: Mix half of liquid B with liquid A, record the volume as C, add semi-solid activator, boil over high heat for 30-45 minutes, simmer the liquid to a volume of D, where the volume D is one third of the volume C, and spray dry to obtain granules;

[0061] Step 4: Mix the remaining half of liquid B with the cleaning solution, boil over high heat, and then simmer over low heat for 0.5 h to obtain the soaking preparation.

[0062] For the Chinese medicine preparations prepared by the methods of Example 1, Example 2 and Example 3, the soaking preparations therein are all used for soaking and hot compressing the roots of white hair to activate the hair root follicles, accelerate the division of hair follicle mother cells, and promote the growth of new hair.

[0063] Comparative test:

[0064] Chimpanzees with gray hair were selected for hair care test, wherein the granules prepared in Example 1 were mixed with warm water and administered to one chimpanzee twice a day, and the soaked preparation was sprayed on the roots of the gray hair of the chimpanzee three times a day, as control group A;

[0065] Two other gorillas were selected to use the granules and soaked preparations prepared in Example 2 and Example 3, respectively, as control group B and control group C;

[0066] After the control group A, control group B and control group C were continuously treated with the drug until the hair turned black, the color of the hair was observed after the drug was stopped. The results are shown in the following table:

[0067]

[0068] Based on the comparison of the above table, it is found that the Chinese medicine preparation prepared in Example 1 is suitable for users with abundant hair and who need to maintain their hair;

[0069] The Chinese medicine preparation prepared in Example 2 is suitable for users who have insufficient hair and need to nourish their hair;

[0070] The Chinese medicine preparation prepared in Example 3 is suitable for users who have hair loss and need to maintain their hair.

[0071] In summary, it can be seen that the Chinese medicine preparations prepared according to the methods of Example 1, Example 2 and Example 3 can not only achieve rapid hair nourishment, but also activate hair follicles to nourish hair roots and further promote hair growth.

[0072] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0073] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Chinese medicine preparation for hair care, characterized in that: The raw materials include, by weight: 5-15 parts of ginger extract, 10-30 parts of wolfberry, 20-50 parts of astragalus, 5-15 parts of codonopsis, 50-150 parts of hair follicle activator, 7-18 parts of Ligustrum lucidum, 12-26 parts of rehmannia root, 5-15 parts of eucommia, 10-30 parts of red dates, 10-30 parts of polygonatum, 10-20 parts of polygonum multiflorum, 20-40 parts of donkey-hide gelatin, 15-40 parts of carrageenan and 5-15 parts of black fungus.

2. A Chinese medicine preparation for hair care according to claim 1, characterized in that: The raw materials include, by weight: 5 parts of ginger extract, 10 parts of wolfberry, 50 parts of astragalus, 15 parts of codonopsis, 50 parts of hair follicle activator, 7 parts of ligustrum lucidum, 26 parts of rehmannia root, 15 parts of eucommia bark, 10 parts of red dates, 30 parts of polygonatum, 10 parts of polygonum multiflorum, 20 parts of donkey-hide gelatin, 40 parts of carrageenan and 5 parts of black fungus.

3. A Chinese medicine preparation for hair care according to claim 1, characterized in that: The raw materials include, by weight: 10 parts of ginger extract, 20 parts of wolfberry, 40 parts of astragalus, 10 parts of codonopsis, 100 parts of hair follicle activator, 12 parts of Ligustrum lucidum, 18 parts of rehmannia root, 10 parts of eucommia bark, 20 parts of red dates, 20 parts of polygonatum, 15 parts of polygonum multiflorum, 30 parts of donkey-hide gelatin, 30 parts of carrageenan and 10 parts of black fungus.

4. A Chinese medicine preparation for hair care according to claim 1, characterized in that: The raw materials include, by weight: 15 parts of ginger extract, 30 parts of wolfberry, 20 parts of astragalus, 5 parts of codonopsis, 150 parts of hair follicle activator, 18 parts of Ligustrum lucidum, 12 parts of rehmannia root, 5 parts of eucommia, 30 parts of red dates, 10 parts of polygonatum, 20 parts of polygonum multiflorum, 40 parts of donkey-hide gelatin, 15 parts of carrageenan, and 15 parts of black fungus.

5. A Chinese medicinal preparation for hair care according to any one of claims 1 to 4, characterized in that: The method for obtaining the ginger extract comprises: Take young ginger planted for four months, peel and crush it, add twice the amount of deionized water, add three times the amount of ethanol, stir well and let it stand for 24-48 hours, and obtain the extract and solid raw ginger after centrifugation; Drying the solid raw material ginger and grinding it into powder to obtain ginger powder; The extract is placed in an ethanol recovery device for heating to recover ethanol, and then the remaining ethanol in the extract is heated to volatilize, ginger powder is added, and the mixture is stirred evenly to obtain ginger extract.

6. A Chinese medicinal preparation for hair care according to any one of claims 1 to 4, characterized in that: The hair follicle activator comprises a cleaning solution and a semi-solid activator, and the preparation method of the cleaning solution and the semi-solid activator comprises: Weigh equal weights of black rice flour, black bean flour, mulberry, black sesame flour, walnut flour and olive oil, place in a heating kettle with a stirring mechanism, add five times the total weight of water and boil over high heat for 1-2 hours, then simmer over low heat until the total volume is reduced to half of the initial volume, let stand for 3-5 hours, vacuum the upper clear liquid to obtain the cleaning liquid, vacuum the remaining liquid to obtain the semi-solid activator.

7. A Chinese medicinal preparation for hair care according to any one of claims 1 to 4, characterized in that: The hair-nourishing Chinese medicinal preparation is a granule preparation and an immersion preparation.

8. A method for preparing a Chinese medicine preparation for hair care, characterized in that: The specific steps include: Step 1, weigh ginger extract, wolfberry, astragalus, codonopsis, hair follicle activator, Ligustrum lucidum, rehmannia root, eucommia bark, red dates, polygonatum, polygonum multiflorum, donkey-hide gelatin, carrageenan and black fungus, the weight ratio is: 5-15:10-30:20-50:5-15:50-150:7-18:12-26:5-15:10-30:10-30:10-20:20-40:15-40:5-15, after screening out the hair follicle activator, obtain the boiling raw materials; Step 2: Add three times the amount of deionized water to the boiled raw materials and boil them over high heat for 2-3 hours, filter out the boiled raw materials to obtain liquid A, add two times the amount of deionized water to the filtered boiled raw materials, boil them over low heat for 1-2 hours, filter out impurities, and obtain liquid B; Step 3: Mix half of liquid B with liquid A, record the volume as C, add semi-solid activator, boil over high heat for 30-45 minutes, simmer the liquid to a volume of D, where the volume D is one third of the volume C, and spray dry to obtain granules; Step 4: Mix the remaining half of liquid B with the cleaning solution, boil over high heat, and then simmer over low heat for 0.5 h to obtain the soaking preparation.