Composition for relieving sub-health and dredging channels and collaterals as well as preparation method and application of composition

Abstract

The invention provides a composition for relieving sub-health and dredging channels and collaterals and a preparation method and application thereof, and belongs to the field of biological agents. According to the composition disclosed by the invention, main components such as squalane, a maguey extract, a polygonum cuspidatum root extract, an angelica root extract, a dandelion extract, a curcuma zedoary extract, a kudzu vine root extract, a caulis spatholobi extract, a golden cypress extract, a liquorice extract, a pepper extract, tocopheryl acetate, isononyl isononanoate, sunflower seed oil and tocopherol are subjected to compatibility; through reasonable compatibility with components for inhibiting inflammation and strengthening skin barrier, the traditional Chinese medicine composition can enhance body microcirculation, promote information exchange and substance exchange between cell tissues, promote meridian cell activation, reduce inflammatory factor level, recover and improve leukocyte quantity, and achieve the purposes of clearing and activating the channels and collaterals and improving human sub-health.

Description

Technical Field

[0001] The present invention belongs to the technical field of biological preparations, and in particular relates to a composition for alleviating sub-health and dredging meridians, and a preparation method and application thereof. Background Art

[0002] Sub-health refers to a state of the human body between health and disease. People in sub-health state often show symptoms of reduced vitality, decreased body function and adaptability for a certain period of time, such as poor mental state, fatigue, depression, irritability, hair loss, sleep disorders, memory loss, palpitations, sweating, soreness of waist and knees, numbness and pain in limbs, menstrual disorders in women, allergies, poor resistance, etc., but they do not meet the clinical or subclinical diagnostic standards of modern medicine for related diseases. In contemporary society, with the accelerated pace of life and increased work pressure, sub-health has become an important health problem that plagues the general public.

[0003] At the same time, with the continuous development of modern science and technology, modern medicine has made breakthrough progress in the research of microscopic fields, and has also provided considerable technical means for the disease research of TCM meridian theory. The introduction of works such as "Introduction to Meridian Medicine", "Vulnerability" and "Collection Diseases" has also made people further aware that meridians are closely related to human health. In this context, dredging meridians, as an important concept in TCM theory, has received increasing attention for its role in improving sub-health status. TCM theory believes that meridians are channels for the circulation of qi and blood in the human body. When meridians are unblocked, qi and blood are harmonized, and the organs of the human body can function normally, thus maintaining a healthy state. On the contrary, blocked meridians will lead to poor qi and blood circulation, causing various diseases or sub-health symptoms. Therefore, by dredging meridians, it is possible to effectively promote the circulation of qi and blood, enhance physical functions, improve immunity, relieve fatigue, and improve mental state, thereby achieving the purpose of improving sub-health status.

[0004] It is generally believed that when the meridians are blocked, the body is prone to the formation of "nodules" (or "knots") due to the blockage of qi and blood. The appearance and touch are different. You can feel small bumps, bubbles, beads, or small hard nodules on the superficial layer of the skin. They can be single or multiple, and of varying sizes. In combination with modern medicine, the causes and types of these "nodules" can be roughly divided into the following categories: (1) Lactic acid nodules, which are caused by the accumulation of lactic acid due to long-term fatigue or excessive exercise. They usually appear in the shoulder and neck area. There is no obvious swelling, but pressing the local area will be accompanied by a sore feeling. Lactic acid nodules can be single or multiple, of varying sizes, with no local redness and swelling, only obvious swelling, similar to soft tissue cysts. (2) Fatty nodules, which are caused by fat metabolism disorders, local microcirculation obstruction, fat accumulation, etc. (3) Fibrous nodules are cross-linked adhesion proteins and synaptic proteins between cells and between cells and the extracellular matrix. They may be scars left after inflammation or skin epidermal treatment. They may also be related to hormone secretion disorders, decreased lymphatic metabolism of body fluids, high-sugar and high-fat diets, and local metabolic waste accumulation. Common examples include lung or breast fibrous nodules and thyroid nodules. (4) Inflammatory nodules are hyperplasia of local tissues caused by long-term inflammatory stimulation. They are usually formed by inflammatory cell infiltration and accumulation of inflammatory mediators. They manifest as nodular hyperplasia and inflammation, including pain, swelling, increased secretion, etc. Nodules can be eliminated through reasonable antibiotic anti-inflammatory treatment, making them smaller or even disappearing. (5) Calcified nodules can be divided into malnutrition calcified nodules and metastatic calcified nodules. The former is usually caused by various reasons that cause pathological or physiological changes in certain organs or tissues of the body, leading to calcium salt deposition, while the latter is often related to systemic calcium metabolism abnormalities, such as hyperparathyroidism leading to abnormal calcium deposition in the body.

[0005] In recent years, with the advancement of science and technology and the increase in people's demand for health, various methods and technologies for dredging meridians have emerged, covering oral, external and physical therapy. Oral products mainly exist in the form of Chinese patent medicines, Chinese herbal medicine slices and various health products, while external products include active oil, essential oils, plasters and patches, etc. At the same time, with the rapid development of science and technology, emerging products such as physical therapy instruments such as infrared therapy instruments, electric pulse massage instruments and acupuncture instruments have also been gradually applied to the treatment field of dredging and dissolving knots, injecting new vitality into traditional therapies.

[0006] However, these treatments all have certain limitations. Although oral medications are convenient, long-term use may increase the metabolic burden on the body; active oils and plasters are mostly limited to local use and have a pungent smell; although essential oil products are good, the active ingredient content is limited, and the skin feels greasy and is prone to allergies; as for physical therapy equipment, it usually requires professional operation and is not convenient for daily home use.

[0007] Therefore, the market urgently needs a meridian dredging product that is both convenient and easy to use and has significant effects, so as to effectively alleviate the sub-health problems commonly faced by people in today's society. Summary of the invention

[0008] In view of this, the purpose of the present invention is to provide a composition for alleviating sub-health and dredging meridians, and its preparation method and application. The composition can significantly improve the sub-health state, enhance the body's immunity, and achieve the purpose of dredging and resolving meridians and improving human sub-health.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] The present invention provides a composition for alleviating sub-health and dredging meridians, comprising the following raw material components in parts by weight:

[0011] 1-20 parts of squalane, 2-10 parts of agave extract, 1-5 parts of knotweed root extract, 1-10 parts of angelica root extract, 2-15 parts of dandelion extract, 7-16 parts of zedoary extract, 4-18 parts of kudzu root extract, 5-15 parts of millettia extract, 3-10 parts of phellodendron extract, 0.01-10 parts of licorice extract, 0.5-15 parts of pepper extract, 0.5-10 parts of tocopheryl acetate, 1-15 parts of isononyl isononanoate, 0.1-5 parts of sunflower seed oil, and 0.1-5 parts of tocopherol.

[0012] Preferably, the composition further comprises one or more of ginseng root extract, ginger root extract, sage oil, bisabolol, motherwort extract, siloxanetriol alginate and caffeine.

[0013] Preferably, the mass fraction of the ginseng root extract is 3 to 5 parts, the mass fraction of the ginger root extract is 1 to 8 parts, the mass fraction of the sage oil is 0.5 to 15 parts, the mass fraction of the bisabolol is 0.5 to 20 parts, the mass fraction of the motherwort extract is 1 to 15 parts, the mass fraction of the siloxane triol alginate is 0.001 to 10 parts, and the mass fraction of the caffeine is 1 to 20 parts.

[0014] The present invention also provides the use of the composition for alleviating sub-health and dredging meridians in the preparation of a medicine for alleviating sub-health.

[0015] The present invention also provides the use of the composition for alleviating sub-health and dredging meridians in the preparation of a medicine for dredging meridians.

[0016] The present invention also provides a preparation comprising the composition for alleviating sub-health and dredging meridians, wherein the dosage form of the preparation includes emulsion, essential oil and cream.

[0017] Preferably, the composition, emulsifier, moisturizer, skin penetration enhancer and water are included, and the mass ratio of the composition, emulsifier, moisturizer, skin penetration enhancer and water is 1-15:1-10:5-20:3-8:30-50.

[0018] Preferably, the emulsifier is one or more of polyoxyethylene castor oil, polyoxyethylene hydrogenated castor oil, glyceryl monostearate, glyceryl oleate, lecithin and Tween; the moisturizer is one or more of glycerol, hyaluronic acid and propylene glycol; the transdermal enhancer is one or more of laurocapram, dimethyl sulfoxide and dimethylacetamide.

[0019] The present invention also provides a method for preparing the preparation for alleviating sub-health and dredging meridians, comprising the following steps:

[0020] (1) mixing agave extract, Polygonum cuspidatum root extract, Angelica sinensis root extract, dandelion extract, Phellodendron amurense extract, Ginseng root extract, Ginger root extract, Leonurus japonicus extract, caffeine, Curcuma zedoaria extract, Pueraria root extract, Millettia spatholobi extract, Piper nigrum extract, bisabolol and water at 40-50° C. to prepare an aqueous phase;

[0021] (2) mixing squalane, tocopheryl acetate, licorice extract, siloxane triol alginate, isononyl isononanoate, sage oil, sunflower seed oil, tocopherol and an emulsifier at 60 to 70° C. to obtain an oil phase;

[0022] (3) The aqueous phase, the oil phase, the moisturizer, and the transdermal enhancer are mixed and stirred, and then homogenized at a high speed of 8000 to 12000 rpm for 3 to 5 minutes to obtain a crude emulsion, and then homogenized at a high pressure for 2 to 3 times to obtain the preparation for alleviating sub-health and unblocking meridians.

[0023] Preferably, the pressure of the high-pressure homogenization is 40 to 100 MPa, the rotation speed of the high-pressure homogenization is 6000 to 10000 rpm, and the time of the high-pressure homogenization is 10 to 20 min.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] Improve body microcirculation: Using angelica root extract, ginseng root extract, ginger root extract, sage oil, motherwort extract, Millettia reticulata extract, kudzu root extract, bisabolol and pepper extract (such as tetrahydropiperine) to promote blood circulation, promote information exchange and material replacement between cell tissues, and enhance the body's metabolic capacity; at the same time, it can increase the fluidity of the lipid bilayer, increase the affinity of drug molecules, nutrients and cell membranes, and help active substances to be absorbed and utilized by cells;

[0026] Improve local fat metabolism disorders: Agave extract and Polygonum cuspidatum root extract are used as the main active ingredients to regulate fat synthesis, increase lipid metabolism levels, and improve the nutritional supply of local tissues and the discharge of metabolic waste;

[0027] It is beneficial to inhibit inflammation and improve resistance: with ginseng root extract, agave extract, licorice extract (such as stearyl glycyrrhizinate), knotweed root extract, tocopherol and tocopherol acetate as the main active ingredients, it can reduce the body's inflammatory response and inhibit the infection and reproduction of infectious pathogens; it can also improve the body's anti-inflammatory and antioxidant capabilities, eliminate free radicals, enhance immunity, and reduce the formation of nodules and swelling;

[0028] Promote the activation of meridian cells: Polygonum cuspidatum root extract and Phellodendron amurense extract are rich in berberine and rhein, which can activate the meridian parenchyma cells - Telocytes, enhance their communication function with surrounding cells, stimulate cell vitality, and thus play a role;

[0029] Unblock lymphatic circulation and improve fibrosis: With dandelion extract, Curcuma zedoaria extract, siloxanetriol alginate and caffeine as the main active ingredients, it promotes lymph circulation, dredges and rebuilds lymphatic channels, prevents collagen fiber hardening, thereby reducing local edema and congestion, and assists in the dissipation of nodules.

[0030] Strengthens the skin barrier: With squalane, isononyl isononanoate, sunflower seed oil and tocopherol as the main ingredients, it nourishes the skin and strengthens the skin's barrier function;

[0031] The preparation for alleviating sub-health and unblocking meridians of the present invention is based on modern Chinese medicine theories such as "Pulse Science" and "Network Diseases", combined with the substantial research of modern medicine on meridians and nodules, and various medicines are used together to play a role in promoting the circulation of qi and blood, activating blood circulation and removing blood stasis, unblocking meridians, unblocking and dissolving nodules, and improving sub-health through reasonable compatibility, enhancing body microcirculation, promoting information exchange and material replacement between cell tissues, improving local fat metabolism disorders, promoting activation of telocytes, inhibiting inflammation and strengthening skin barriers. The composition of the present invention is applied to the affected area, combined with simple Chinese medicine meridian manipulation, so that the drug penetrates into the skin, is absorbed in a targeted manner, and the effect of improving sub-health is achieved by unblocking the meridians. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The neck dredging effect of the preparation of the present invention on Case 1 in Experimental Example 3;

[0033] Figure 2 The effect of the preparation of the present invention on the neck dredging of case 2 in experimental example 3;

[0034] Figure 3The effect of the preparation of the present invention on the shoulder and neck dredging of Case 3 in Experimental Example 3;

[0035] Figure 4 The effect of the preparation of the present invention on the neck and shoulder dredging of case 4 in experimental example 3;

[0036] Figure 5 The effect of the preparation of the present invention on the neck and shoulder dredging of case 5 in experimental example 3;

[0037] Figure 6 The effect of the preparation of the present invention on the underarm dredging of Case 6 in Experimental Example 3 is shown. DETAILED DESCRIPTION

[0038] The present invention provides a composition for alleviating sub-health and dredging meridians, comprising the following raw material components in parts by weight:

[0039] 1-20 parts of squalane, more preferably 5-15 parts, more preferably 10 parts; 2-10 parts of agave extract, more preferably 4-8 parts, more preferably 6 parts; 1-5 parts of Polygonum cuspidatum root extract, more preferably 2-4 parts, more preferably 3 parts; 1-10 parts of Angelica dahurica root extract, more preferably 3-8 parts, more preferably 5 parts; 2-15 parts of dandelion extract, more preferably 5-12 parts, more preferably 8 parts; 7-16 parts of Curcuma zedoaria extract, more preferably 10-14 parts, more preferably 12 parts; 4-18 parts of Pueraria extract, more preferably 8-15 parts, more preferably 10 parts; 5-15 parts of Millettia spatholobi extract, more preferably 7 to 11 parts, more preferably 9 parts; 3 to 10 parts of Phellodendron chinense extract, more preferably 5 to 8 parts, more preferably 7 parts; 0.01 to 10 parts of licorice extract, more preferably 3 to 8 parts, more preferably 5 parts; 0.5 to 15 parts of pepper extract, more preferably 5 to 12 parts, more preferably 9 parts; 0.5 to 10 parts of tocopherol acetate, more preferably 3 to 7 parts, more preferably 5 parts; 1 to 15 parts of isononyl isononanoate, more preferably 4 to 12 parts, more preferably 8 parts; 0.1 to 5 parts of sunflower seed oil, more preferably 2 to 4 parts, more preferably 3 parts; 0.1 to 5 parts of tocopherol, more preferably 2 to 4 parts, more preferably 3 parts.

[0040] In the present invention, one or more of ginseng root extract, ginger root extract, sage oil, bisabolol, motherwort extract, siloxanetriol alginate and caffeine are also included.

[0041] In the present invention, the mass fraction of the ginseng root extract is 3 to 5 parts, more preferably 3.5 to 4.5 parts, and more preferably 4 parts; the mass fraction of the ginger root extract is 1 to 8 parts, more preferably 3 to 6 parts, and more preferably 5 parts; the mass fraction of the sage oil is 0.5 to 15 parts, more preferably 5 to 10 parts, and more preferably 7 parts; the mass fraction of the bisabolol is 0.5 to 20 parts, more preferably 5 to 15 parts, and more preferably 10 parts; the mass fraction of the motherwort extract is 1 to 15 parts, more preferably 5 to 10 parts, and more preferably 8 parts; the mass fraction of the siloxane triol alginate is 0.001 to 10 parts, more preferably 2 to 7 parts, and more preferably 5 parts; the mass fraction of the caffeine is 1 to 20 parts, more preferably 5 to 15 parts, and more preferably 10 parts.

[0042] Squalane is a hydrocarbon oil extracted from shark liver or synthesized through plant oils. It is widely involved in physiological activities such as cholesterol biosynthesis, biological oxidation and metabolism. It has the functions of strengthening the repair of the epidermis, participating in the construction of skin barrier, delaying skin aging, promoting blood circulation, and assisting in repairing damaged cells.

[0043] Agave extract is a natural antioxidant that can remove free radicals in the body, reduce the damage to cells caused by oxidative stress, effectively inhibit bacterial growth, has antibacterial and anti-inflammatory effects, helps protect the integrity of cell structure, promotes normal cell metabolism, and thus indirectly promotes the smooth operation of various systems in the body.

[0044] The root extract of Polygonum cuspidatum contains active ingredients such as resveratrol and rhamnolide, which have anti-inflammatory, antibacterial, antioxidant and blood circulation promoting effects. Resveratrol can induce antioxidant gene expression, reduce the production of cellular active oxygen free radicals and scavenge oxygen free radicals, thereby alleviating inflammatory response and promoting tissue repair and regeneration; rhamnolide and its derivatives can destroy the integrity of bacterial cell membranes, inhibit the release of inflammatory factors, reduce tissue inflammatory response, and inhibit the growth and metastasis of tumor cells. It has a certain effect of clearing and dissolving lumps and tumors caused by inflammation or tumors.

[0045] Angelica root extract can improve blood circulation, promote blood flow, reduce inflammatory response, reduce tissue edema and exudation, accelerate cell metabolism, thereby eliminating pathological changes such as agglomerations and lumps caused by blood stasis, and promote the repair and regeneration of damaged tissues. It has the effects of promoting blood circulation, removing blood stasis, promoting metabolism and anti-inflammatory.

[0046] Millettia spatholobi extract can stimulate bone marrow hematopoiesis and increase hemoglobin content, thereby improving blood stasis symptoms and promoting blood circulation, making blood flow unimpeded in the meridians, and has good inflammation inhibition and lipid-lowering effects. "Collection Diseases" records that Millettia spatholobi has significant effects on hemiplegia or limb numbness caused by stroke, rheumatic arthralgia, limb weakness or numbness and swelling.

[0047] Pueraria extract is rich in puerarin and daidzein. Puerarin can act on vascular smooth muscle cells to dilate them, thereby dilating blood vessels and increasing blood flow, while reducing blood viscosity and platelet aggregation, thereby improving blood fluidity and promoting blood circulation; daidzein can bind to acetylcholine receptors, thereby blocking the contraction of acetylcholine on smooth muscle, helping to relieve spasms and relieve muscle tension and pain, and improve muscle discomfort. In "Treatise on Febrile Diseases", kudzu root soup is also often used to dispel wind and cold, sweat and relieve muscles, relieve pain and activate collaterals.

[0048] Dandelion extract can inhibit inflammatory responses in the body, reduce swelling and the formation of nodules, and has the effects of clearing away heat and detoxifying, promoting diuresis and relieving stranguria, and enhancing the body's immunity.

[0049] Phellodendron chinense extract has the effects of clearing away heat and dampness, detoxifying and treating sores, inhibiting bacteria and inflammation, improving endocrine, promoting blood circulation and tissue repair.

[0050] Ginseng root extract contains active ingredients such as ginsenosides and ginseng polysaccharides, which can enhance the body's immune function, promote blood circulation, inhibit the release of inflammatory factors, reduce tissue swelling and edema caused by inflammation, eliminate free radicals in the body, and reduce oxidative damage. It has anti-inflammatory, antioxidant, anti-aging, improves blood circulation, and enhances immune function.

[0051] Ginger root extract contains active ingredients such as 6-gingerol, which can effectively relieve pain and swelling caused by inflammatory diseases such as arthritis and muscle pain, reduce the damage of oxidative stress to the body, and promote local blood circulation, thereby improving blood circulation and nutrient supply to local tissues, and preventing and treating diseases caused by oxidative stress.

[0052] Motherwort extract can promote blood production and circulation, helping the body recover and regulate its function.

[0053] Stearyl glycyrrhizinate is the active ingredient in licorice extract. It can promote the excretion of heat evil in the body, increase the metabolic rate, reduce tissue inflammation, promote the repair and regeneration of damaged tissues, and has anti-inflammatory, detoxifying, and dredging effects.

[0054] Pepper extract is rich in active ingredients such as piperine, which can disturb the phospholipid bilayer of the cell membrane, promote the transdermal absorption and diffusion of active ingredients, and enhance the affinity between active ingredients and cell membranes.

[0055] Tocopherol acetate can protect cells from oxidative damage by stabilizing cell membrane structure and reducing free radical production, inhibit the release of inflammatory mediators and reduce tissue edema, accelerate epithelial cell proliferation and granulation tissue formation, inhibit the fibrosis process to a certain extent, and has anti-inflammatory, antioxidant and tissue healing effects.

[0056] Isononyl isononanoate can enhance skin barrier function and antioxidant capacity, promoting skin health.

[0057] The unsaturated fatty acids and vitamin E contained in sunflower seed oil can form a protective film on the skin surface, effectively preventing moisture evaporation and keeping the skin permanently hydrated. At the same time, it can relieve skin inflammation and redness, reduce skin discomfort caused by external stimuli, promote the repair of damaged skin, and has certain anti-inflammatory properties.

[0058] Tocopherol can neutralize free radicals in the body, reduce cell damage, slow down the aging process, promote cell regeneration and repair, and protect cardiovascular health by reducing the oxidation of low-density lipoprotein cholesterol. It has antioxidant, cardiovascular protection, and immunity-enhancing effects.

[0059] Medicinal sage oil can promote blood circulation and lymph flow, relieve symptoms such as swelling, pain and nodules caused by inflammation, eliminate local swelling and pain caused by infection, promote tissue repair and regeneration, and has the effects of promoting blood circulation and removing blood stasis, anti-inflammatory and antibacterial, and reducing swelling and relieving pain.

[0060] Bisabolol is a sesquiterpene alcohol compound extracted from pure natural plants. It has good skin compatibility and stability, can relieve pain, swelling and nodules caused by blood stasis, reduce inflammatory response, relieve muscle and bone pain, improve limited joint movement, scavenge free radicals, delay cell aging, and has blood circulation, anti-inflammatory and swelling, antibacterial and antioxidant effects. It has a certain auxiliary therapeutic effect on symptoms such as rheumatoid arthritis, skin inflammation, and muscle soreness.

[0061] Siloxane triol alginate is a silicone derivative produced by hydrogen bonding of monomer silicon and active groups. It is soluble in water and can be better absorbed by the skin. It can effectively supplement the lack of silicon in the body, activate hydroxylase and collagen synthesis, help the formation of extracellular matrix structure, increase the elasticity and strength of connective tissue, and prevent collagen fiber hardening.

[0062] Caffeine is a methylxanthine compound that can relieve fatigue, promote metabolism, increase basal metabolic rate and relieve pain.

[0063] The extract of Curcuma is rich in a variety of olefins. It is recorded in the Compendium of Materia Medica that "the function of Curcuma is to promote qi, break blood and disperse stagnation". Modern medical research shows that Curcuma has an anti-fibrosis effect, and can also change blood rheology parameters such as whole blood viscosity, hematocrit, erythrocyte sedimentation rate, and reduced viscosity, prevent platelet aggregation, inhibit in vitro thrombosis, and promote blood flow.

[0064] The present invention also provides the use of the composition for alleviating sub-health and dredging meridians in the preparation of a medicine for alleviating sub-health.

[0065] The present invention also provides the use of the composition for alleviating sub-health and dredging meridians in the preparation of a medicine for dredging meridians.

[0066] The present invention also provides a preparation comprising the composition, wherein the dosage forms of the preparation include emulsion, essential oil and cream.

[0067] In the present invention, the composition, emulsifier, moisturizer, transdermal enhancer and water are included, and the mass ratio of the composition, emulsifier, moisturizer, transdermal enhancer and water is 1-15:1-10:5-20:3-8:30-50.

[0068] In the present invention, the mass ratio of the composition, emulsifier, moisturizer, transdermal enhancer and water is preferably 1-15:1-10:5-20:3-8:30-50, more preferably 5-10:3-8:8-17:4-7:35-45, and even more preferably 8:5:13:5:40.

[0069] In the present invention, the emulsifier includes one or more of polyoxyethylene castor oil, polyoxyethylene hydrogenated castor oil, glyceryl monostearate, glyceryl oleate, lecithin and Tween; the moisturizer includes one or more of glycerol, hyaluronic acid and propylene glycol; the transdermal enhancer is preferably one or more of laurocapram, dimethyl sulfoxide and dimethylacetamide.

[0070] The present invention also provides a method for preparing the preparation for alleviating sub-health and dredging meridians, comprising the following steps:

[0071] (1) mixing agave extract, Polygonum cuspidatum root extract, Angelica sinensis root extract, dandelion extract, Phellodendron amurense extract, Ginseng root extract, Ginger root extract, Leonurus japonicus extract, caffeine, Curcuma zedoaria extract, Pueraria root extract, Millettia spatholobi extract, Piper nigrum extract, bisabolol and water at 40-50° C. to prepare an aqueous phase;

[0072] (2) mixing squalane, tocopheryl acetate, licorice extract, siloxane triol alginate, isononyl isononanoate, sage oil, sunflower seed oil, tocopherol and an emulsifier at 60 to 70° C. to obtain an oil phase;

[0073] (3) The aqueous phase, the oil phase, the moisturizer, and the transdermal enhancer are mixed and stirred, and then homogenized at 8000-12000 rpm for 3-5 minutes to obtain a crude emulsion, and then high-pressure homogenized for 2-3 times to obtain the preparation for alleviating sub-health and unblocking meridians.

[0074] In the present invention, agave extract, polygonum cuspidatum root extract, angelica root extract, dandelion extract, phellodendron chinense extract, ginseng root extract, ginger root extract, motherwort extract, caffeine, zedoary turmeric extract, kudzu root extract, millettia spatholobi extract, piperine extract, bisabolol and water are mixed and stirred to prepare an aqueous phase, and the mixing temperature is 60-70°C, more preferably 62-68°C, and more preferably 65°C.

[0075] In the present invention, squalane, tocopherol acetate, licorice extract, siloxane triol alginate, isononyl isononanoate, sage oil, sunflower seed oil, tocopherol and an emulsifier are uniformly mixed to obtain an oil phase; the mixing temperature is 60-70°C, more preferably 62-68°C, and more preferably 65°C.

[0076] In the present invention, the aqueous phase and the oil phase are mixed and stirred, and then high-speed homogenized at 8000-12000 rpm for 3-5 minutes to obtain a crude emulsion, and then high-pressure homogenized 2-3 times to obtain the preparation for alleviating sub-health and dredging meridians. The speed of the high-speed homogenization is 8000-12000 rpm, preferably 9000-11000 rpm, and more preferably 10000 rpm; the time of high-speed homogenization is 3-5 minutes, preferably 4 minutes;

[0077] The temperature of the high-pressure homogenization is 60-70°C, preferably 62-68°C, and more preferably 65°C; the pressure of the high-pressure homogenization is 40-100MPa, preferably 60-80MPa, and more preferably 70MPa; the rotation speed of the high-pressure homogenization is preferably 6000-10000rpm, more preferably 7000-9000rpm, and more preferably 8000rpm; the time of the high-pressure homogenization is 10-20min, preferably 12-18min, and more preferably 15min; the number of high-pressure homogenization is 2-3 times, and preferably 3 times; the high-pressure homogenization is preferably carried out in a high-pressure homogenizer.

[0078] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the scope of protection of the present invention. The raw materials of the composition and preparation for alleviating sub-health and dredging meridians of the present invention can be purchased through commercial channels unless otherwise specified.

[0079] The test mice of the present invention were 8-week-old SD male rats purchased from Beijing Weitonglihua Experimental Animal Technology Co., Ltd.

[0080] The test strain of the present invention is Propionibacterium acnes ATCC6919 strain, which is from the Institute of Microbiology of Guangdong Academy of Sciences;

[0081] In the kit of the present invention, the rat interleukin 10 (IL-10) ELISA kit and the rat transforming growth factor β (TGF-β) ELISA kit were purchased from Wuhan Gilead Biotechnology Co., Ltd.

[0082] Example 1

[0083] A composition for alleviating sub-health and dredging meridians, comprising the following raw material components:

[0084] Squalane 10g, agave extract 6g, knotweed root extract 3g, angelica root extract 5g, dandelion extract 8g, zedoary extract 12g, kudzu root extract 10g, millettia extract 9g, phellodendron extract 7g, licorice extract 5g, pepper extract 9g, tocopheryl acetate 5g, isononyl isononanoate 8g, sunflower seed oil 3g, tocopherol 3g, ginseng root extract 4g, ginger root extract 5g, sage oil 7g, bisabolol 10g, motherwort extract 8g, siloxanetriol alginate 5g, caffeine 10g.

[0085] The preparation method of the preparation for alleviating sub-health and dredging meridians in this embodiment comprises the following steps:

[0086] (1) thoroughly mixing agave extract, Polygonum cuspidatum root extract, Angelica sinensis root extract, dandelion extract, Phellodendron amurense extract, Ginseng root extract, Ginger root extract, Leonurus japonicus extract, caffeine, Curcuma zedoaria extract, Pueraria root extract, Millettia spatholobi extract, Piper nigrum extract, bisabolol and 760 g of water at 60° C. to prepare an aqueous phase;

[0087] (2) mixing squalane, tocopheryl acetate, licorice extract, siloxane triol alginate, isononyl isononanoate, sage oil, sunflower seed oil, tocopherol and 95 g of polyoxyethylene hydrogenated castor oil at 60° C. to obtain an oil phase;

[0088] (3) The aqueous phase, the oil phase, 247 g of hyaluronic acid, and 95 g of laurocapram are mixed and stirred, and then homogenized at high speed at 10,000 rpm for 4 min, and then homogenized at high pressure at 65° C., 70 MPa, and 8,000 rpm for 10 min. The high-pressure homogenization is repeated three times to obtain the preparation for alleviating sub-health and unblocking meridians.

[0089] Example 2

[0090] A composition for alleviating sub-health and dredging meridians, comprising the following raw material components in parts by weight:

[0091] Squalane 10g, agave extract 6g, knotweed root extract 3g, angelica root extract 5g, dandelion extract 8g, zedoary extract 12g, kudzu root extract 10g, millettia extract 9g, phellodendron extract 7g, licorice extract 5g, pepper extract 9g, tocopheryl acetate 5g, isononyl isononanoate 8g, sunflower seed oil 3g, tocopherol 3g, ginseng root extract 4g, ginger root extract 5g, bisabolol 10g, siloxanetriol alginate 5g, caffeine 10g.

[0092] The preparation method of the preparation for alleviating sub-health and dredging meridians in this embodiment comprises the following steps:

[0093] (1) thoroughly mixing agave extract, Polygonum cuspidatum root extract, Angelica sinensis root extract, Taraxacum officinale extract, Phellodendron amurense extract, Ginseng root extract, Ginger root extract, caffeine, Curcuma zedoaria extract, Pueraria root extract, Millettia spatholobi extract, Piper nigrum extract, bisabolol and 685 g of water at 65° C. to prepare an aqueous phase;

[0094] (2) squalane, tocopheryl acetate, licorice extract, siloxanetriol alginate, isononyl isononanoate, sunflower seed oil, tocopherol and 85.625 g of glyceryl oleate were thoroughly mixed at 65° C. to obtain an oil phase;

[0095] (3) The aqueous phase, the oil phase, 222.625 g of propylene glycol, and 85.625 g of dimethyl sulfoxide were mixed and stirred, and then homogenized at high speed at 11000 rpm for 3 min, and then homogenized at high pressure at 68° C., 90 MPa, and 9000 rpm for 15 min. The high pressure homogenization was repeated 3 times to obtain the preparation for alleviating sub-health and unblocking meridians.

[0096] Example 3

[0097] A composition for alleviating sub-health and dredging meridians, comprising the following raw material components in parts by weight:

[0098] Squalane 10g, agave extract 6g, knotweed root extract 3g, angelica root extract 5g, dandelion extract 8g, zedoary extract 12g, kudzu root extract 10g, millettia extract 9g, phellodendron extract 7g, licorice extract 5g, pepper extract 9g, tocopheryl acetate 5g, isononyl isononanoate 8g, sunflower seed oil 3g, tocopherol 3g, sage oil 7g, motherwort extract 8g, siloxanetriol alginate 5g, caffeine 10g.

[0099] The preparation method of the preparation for alleviating sub-health and dredging meridians in this embodiment comprises the following steps:

[0100] (1) thoroughly mixing agave extract, Polygonum cuspidatum root extract, Angelica sinensis root extract, dandelion extract, Phellodendron amurense extract, Leonurus japonicus extract, caffeine, Curcuma zedoaria extract, Pueraria root extract, Millettia spatholobi extract, Piper nigrum extract and 665 g of water at 70° C. to prepare an aqueous phase;

[0101] (2) Squalane, tocopheryl acetate, licorice extract, siloxane triol alginate, isononyl isononanoate, sage oil, sunflower seed oil, tocopherol and 83.125 g of lecithin were fully mixed at 70° C. to obtain an oil phase;

[0102] (3) The aqueous phase, the oil phase, 216.125 g of glycerol and 83.125 g of dimethylacetamide were mixed and stirred, and then homogenized at 9000 rpm for 3 min, and then homogenized at high pressure for 20 min at 60° C., 100 MPa and 10000 rpm. The high pressure homogenization was repeated 3 times to obtain the preparation for alleviating sub-health and unblocking meridians.

[0103] Comparative Example 1

[0104] The preparation was prepared by the method of Example 1, except that in this comparative example, angelica root extract, ginseng root extract, ginger root extract, sage oil, bisabolol and motherwort extract were not added.

[0105] Comparative Example 2

[0106] The preparation was prepared by the method of Example 1, except that in this comparative example, dandelion extract, siloxane triol alginate and caffeine were not added.

[0107] Comparative Example 3

[0108] The preparation was prepared by the method of Example 1, except that, in this comparative example, no Polygonum cuspidatum root extract and Phellodendron amurense extract were added.

[0109] Comparative Example 4

[0110] The preparation was prepared by the method of Example 1, except that in this comparative example, no Curcuma zedoaria extract, Pueraria root extract and Millettia spatholobi extract were added.

[0111] Experimental Example 1: Analysis of inflammatory factors in rats with skin inflammatory nodules model

[0112] 90 SD male rats were depilated on their back skin (depilation area was 2 cm×2 cm) and divided into 9 groups, namely, control group, model group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group, with 10 rats in each group; referring to the method disclosed in the literature by Hyun et al. (Jang YH, Lee KC, Lee SJ, et al. HR-1 mice: A new inflammatory acne mouse model [J]. Ann Dermatol, 2015, 27 (3): 257-264), the depilated back of the mice in the model group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group were subcutaneously inoculated with Propionibacterium acnes ATCC6919 strain to establish a rat skin inflammatory nodule model; the rats in the control group were not inoculated with the strain. One week after successful modeling, the preparations prepared in Examples 1 to 3 and Comparative Examples 1 to 4 were applied to the back skin of rats in Example 1 to 3 groups and Comparative Examples 1 to 4 groups, respectively, once a day, 200 mg each time, and applied continuously for 3 weeks. The IL-10 and TGF-β contents of rats in each group were detected using a rat interleukin 10 (IL-10) ELISA kit and a rat transforming growth factor β (TGF-β) ELISA kit. The results are shown in Table 1.

[0113] Table 1 The content of inflammatory factors in the back skin tissue of rats in each group

[0114] Group IL-10 content (ng / mL) TGF-β content (pg / mL) Control group 13.25 213.96 Model Group 58.44 560.22 Example 1 Group 16.35 307.81 Example 2 Group 18.22 273.11 Example 3 Group 16.94 286.3 Comparative Example 1 24.36 353.88 Comparative Example 2 30.71 376.04 Comparative Example 3 34.59 368.57 Comparative Example 4 38.63 392.89

[0115] The results showed that compared with the model group rats, after administration of the preparations prepared in Examples 1 to 3, the IL-10 and TGF-β levels in the skin inflammatory nodule model rats were significantly reduced, indicating that the preparations of the present invention can effectively improve the inflammation level in the skin inflammatory nodules; while the preparations prepared in Comparative Examples 1 to 4 had poor therapeutic effects on inflammation in the skin inflammatory nodules of rats.

[0116] Experimental Example 2: Analysis of inflammatory cell infiltration in rats with skin inflammatory nodules model

[0117] After the rats in each group in Experimental Example 1 were killed, 20 μL of peripheral whole blood was taken respectively, 380 μL of leukocyte diluent was added and mixed thoroughly, and then filled into a cell counting plate to count the leukocytes and calculate the ratio. The results are shown in Table 2.

[0118] Table 2 Peripheral blood leukocyte counts of rats in each group

[0119] Group Neutrophil ratio (%) Lymphocyte ratio (%) Monocyte ratio (%) Control group 12.33 86.25 4.01 Model Group 21.35 64.66 4.93 Example 1 Group 13.15 83.15 4.09 Example 2 Group 13.26 82.36 4.11 Example 3 Group 13.71 80.48 4.12 Comparative Example 1 18.64 68.71 4.57 Comparative Example 2 18.22 67.35 4.64 Comparative Example 3 19.32 68.9 4.69 Comparative Example 4 18.61 66.11 4.55

[0120] The results showed that compared with the model group rats, after administration of the preparations prepared in Examples 1 to 3, the number of neutrophils and monocytes in the skin inflammatory nodule model rats was significantly reduced, while the number of lymphocytes was significantly increased, indicating that the preparation of the present invention can restore and improve the number of white blood cells in rats, thereby effectively inhibiting inflammation, promoting lymphatic circulation, and thus improving the body resistance of rats.

[0121] Experimental Example 3: Clinical Effect Case

[0122] Case 1

[0123] Ms. Gao from Fujian, 40 years old, has a thick neck, loose skin, obvious neck lines, unclear facial contours, and deep nasolabial folds. Care area: Neck. The dosage of the preparation (prepared according to the method of Example 1) is 100g each time, and the care frequency is once every 3 to 5 days for 3 months. After using the preparation of the present invention for care, the neck becomes thinner, the facial contour is obvious, the skin becomes firm and delicate, and the nasolabial folds become shallow. The comparison before and after use is as follows Figure 1 shown.

[0124] Case 2

[0125] Mr. Li from Baotou, Inner Mongolia, aged 46, had a swollen and tight neck, slow metabolism, and a more obvious granular feel to the touch, and his neck was stiff. Part of the neck to be cared for: Neck. The dosage of the preparation (prepared according to the method of Example 2) was 50 g each time, and the frequency of care was once every 5 days for 3 consecutive months. After care with the preparation of the present invention, the tension in the neck was significantly improved, and he felt a lot more relaxed. The patient persisted in doing this for 3 months, and his neck became significantly smaller. When he touched his neck with his hand, no obvious granular feeling was found, and the entire neck became soft and flexible. The comparison before and after use is shown in the figure below. Figure 2 shown.

[0126] Case 3

[0127] Ms. Wang from Yunnan, aged 53, had a fat hyperplastic mass at the Dazhui point of her neck. She often felt numbness, swelling and pain, and had tried many treatments but could not improve the condition. Nursing area: shoulder and neck. The dosage of each preparation (prepared according to the method of Example 3) is 20g, and the nursing frequency is once a week. She said that she could feel numbness after each massage, and the swelling and pain disappeared after the massage, and her shoulder and neck felt very comfortable. After 4 months of continuous nursing, the mass became significantly smaller, the pile of fat on the neck became significantly smaller, and the swelling and pain did not reappear. The comparison before and after use is as follows Figure 3 shown.

[0128] Case 4

[0129] Ms. Zhong from Shandong, aged 48, suffered from severe neck and shoulder congestion, frequent dizziness, insufficient blood supply and poor sleep quality. Each dose of the preparation (prepared according to the method of Example 1) was 20g, and the nursing frequency was once a week for 3 consecutive months. After 3 months, she obviously felt relaxed, her sleep improved, and her neck contour improved and her neck lines were relaxed. The comparison before and after use is shown in the figure below. Figure 4 shown.

[0130] Case 5

[0131] Ms. Shao from Zhejiang, aged 27, had horizontal lines on her neck and a fat hyperplastic mass on her neck. The dosage of each preparation (prepared according to the method of Example 2) was 100g, and the treatment frequency was 1 to 2 times a week for 6 consecutive months. After 6 months, after the first treatment, the horizontal lines on the neck were significantly faded, the mass softened, and at the same time, the tension in the entire shoulder and neck was relieved, the shoulder and neck turned more flexibly, the skin became firm and elastic, and the warmth was more lasting. After 6 months, when viewed from the side, the horizontal lines on the back of the neck could not be seen, and the mass on the neck became flat. The comparison before and after use is shown in the figure below. Figure 5 shown.

[0132] Case 6

[0133] Ms. Liu from Jiangsu, 50 years old, had a lump in her armpit and distended pain 4-5 days before her period. Each dose of the preparation (prepared according to the method of Example 3) was 100g, and the treatment frequency was once a week for 3 months. After 3 months, the lump in her armpit was significantly reduced, and the distended pain before her period disappeared. The comparison before and after use is shown in the figure below. Figure 6 shown.

[0134] Case 7

[0135] Ms. Han from Guangdong, 31 years old, has been sitting for a long time, has obesity in the waist and abdomen, and has obvious edema in the lower limbs. She often feels heavy and weak in the lower limbs, and the leg muscles are hard and accompanied by soreness. When she touches the leg muscles, she can feel that they are not smooth and have granular substances. Use the preparation of the present invention (prepared according to the method of Example 1) to massage the abdomen and legs, 100g each time, once a week, and continuously care for 4 times. The abdominal skin becomes firm, and the waist circumference is reduced from 73.5cm to 64.3cm; the edema and heaviness of the legs disappear significantly, the muscles become soft and elastic, the granularity of the legs disappears, and the whole person is much lighter.

[0136] Case 8

[0137] Ms. Sun, 37 years old, went to the hospital for examination because of neck swelling and discomfort, foreign body sensation when swallowing, and frequent hoarseness. Physical examination revealed a 7mm×6mm nodule in the right lobe of the thyroid gland, TI-RADS category 3. The preparation of the present invention (prepared according to the method of Example 2) was used to care for the thyroid gland and shoulder and neck, with a dosage of 30g each time, twice a week. After 3 months of care, ultrasound was performed again, and the nodule became 2mm×1mm, the foreign body sensation and swelling in the neck disappeared, and the voice returned to normal.

[0138] Case 9

[0139] Mr. Pang went to the hospital for a checkup one month ago because of a nodule in the front of the neck accompanied by distension and pain, and discomfort in the throat. A cystic-solid nodule of 19mm×14mm was found on the left side of the thyroid gland, which was TI-RADS2. The preparation of the present invention (prepared according to the method of Example 3) was used to care for the thyroid gland and shoulder and neck, with a dosage of 30g each time, and care was given twice a week. After care for 3 months, ultrasound was performed again, and the nodule had become 19mm×15mm; after the patient continued care for 6 months, the nodule had become 19mm×9mm, and the distension and pain in the neck and discomfort in the throat had significantly disappeared.

[0140] From the specific cases in Experimental Example 3, it can be seen that the preparation of the present invention has the effect of promoting body circulation and unblocking knots. Specifically, it can effectively improve problems such as neck swelling, fat hyperplasia, shoulder and neck congestion, armpit lumps, and heaviness of lower limbs, and improve physical discomfort.

[0141] As can be seen from the above embodiments, the present invention provides a composition for alleviating sub-health and unblocking meridians, as well as a preparation method and application thereof. The composition prepared by the present invention and its preparation have the effects of promoting blood circulation and removing blood stasis, unblocking meridians and activating collaterals, and resisting fatigue. They can reduce the level of inflammatory factors in skin inflammatory nodules, restore and improve the number of white blood cells, promote body microcirculation, promote information exchange and material replacement between cell tissues, and enhance the body's immune function. In clinical studies, the biological preparations of the present invention can significantly relieve various sub-health problems caused by different meridians, such as neck swelling, fat hyperplasia, shoulder and neck congestion, axillary masses, and heaviness of the lower limbs; in addition, the biological preparations of the present invention can also effectively improve the size of nodules in clinical patients with thyroid nodules, and help prevent and alleviate thyroid nodule-related diseases.

[0142] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition for alleviating sub-health and dredging meridians, characterized in that: The composition includes the following components in parts by weight: 1-20 parts of squalane, 2-10 parts of agave extract, 1-5 parts of knotweed root extract, 1-10 parts of angelica root extract, 2-15 parts of dandelion extract, 7-16 parts of zedoary extract, 4-18 parts of kudzu root extract, 5-15 parts of millettia extract, 3-10 parts of phellodendron extract, 0.01-10 parts of licorice extract, 0.5-15 parts of pepper extract, 0.5-10 parts of tocopheryl acetate, 1-15 parts of isononyl isononanoate, 0.1-5 parts of sunflower seed oil, and 0.1-5 parts of tocopherol.

2. The composition for alleviating sub-health and dredging meridians according to claim 1, characterized in that: It also includes one or more of ginseng root extract, ginger root extract, sage oil, bisabolol, motherwort extract, siloxanetriol alginate and caffeine.

3. The composition for alleviating sub-health and dredging meridians according to claim 2, characterized in that: The mass fraction of the ginseng root extract is 3 to 5 parts, the mass fraction of the ginger root extract is 1 to 8 parts, the mass fraction of the sage oil is 0.5 to 15 parts, the mass fraction of the bisabolol is 0.5 to 20 parts, the mass fraction of the motherwort extract is 1 to 15 parts, the mass fraction of the siloxane triol alginate is 0.001 to 10 parts, and the mass fraction of the caffeine is 1 to 20 parts.

4. Use of the composition for alleviating sub-health and dredging meridians according to any one of claims 1 to 3 in the preparation of a drug for alleviating sub-health.

5. Use of the composition for alleviating sub-health and dredging meridians according to any one of claims 1 to 3 in the preparation of a medicament for dredging meridians.

6. A preparation comprising the composition for alleviating sub-health and dredging meridians according to any one of claims 1 to 3, characterized in that: The dosage forms of the preparation include emulsion, essential oil and cream.

7. The preparation for alleviating sub-health and dredging meridians according to claim 6, characterized in that: The invention comprises the composition according to any one of claims 1 to 3, an emulsifier, a moisturizer, a transdermal enhancer and water, wherein the mass ratio of the composition according to any one of claims 1 to 3, the emulsifier, the moisturizer, the transdermal enhancer and water is 1 to 15:1 to 10:5 to 20:3 to 8:30 to 50.

8. The preparation for alleviating sub-health and dredging meridians according to claim 7, characterized in that: The emulsifier is one or more of polyoxyethylene castor oil, polyoxyethylene hydrogenated castor oil, glyceryl monostearate, glyceryl oleate, lecithin and Tween; the moisturizer is one or more of glycerol, hyaluronic acid and propylene glycol; the transdermal enhancer is one or more of laurocapram, dimethyl sulfoxide and dimethylacetamide.

9. The method for preparing the preparation for alleviating sub-health and dredging meridians according to any one of claims 6 to 8, characterized in that: The steps include: (1) mixing agave extract, Polygonum cuspidatum root extract, Angelica sinensis root extract, dandelion extract, Phellodendron amurense extract, Ginseng root extract, Ginger root extract, Leonurus japonicus extract, caffeine, Curcuma zedoaria extract, Pueraria root extract, Millettia spatholobi extract, Piper nigrum extract, bisabolol and water at 40-50° C. to prepare an aqueous phase; (2) mixing squalane, tocopheryl acetate, licorice extract, siloxane triol alginate, isononyl isononanoate, sage oil, sunflower seed oil, tocopherol and an emulsifier at 60 to 70° C. to obtain an oil phase; (3) The aqueous phase, the oil phase, the moisturizer, and the transdermal enhancer are mixed and stirred, and then homogenized at a high speed of 8000 to 12000 rpm for 3 to 5 minutes to obtain a crude emulsion, and then homogenized at a high pressure for 2 to 3 times to obtain the preparation for alleviating sub-health and unblocking meridians.

10. The preparation method according to claim 9, characterized in that: The pressure of the high-pressure homogenization is 40-100 MPa, the rotation speed of the high-pressure homogenization is 6000-10000 rpm, and the time of the high-pressure homogenization is 10-20 min.

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